研究者詳細 - 石川　健治

論文 - 石川　健治

分割表示 424 件中 241 - 260 件目／全件表示 >>

Cytotoxicity of cancer HeLa cells sensitivity to normal MCF10A cells in cultivations with cell culture medium treated by microwave-excited atmospheric pressure plasmas 査読有り

Takahashi, Y; Taki, Y; Takeda, K; Hashizume, H; Tanaka, H; Ishikawa, K; Hori, M

JOURNAL OF PHYSICS D-APPLIED PHYSICS 51 巻 ( 11 ) 2018年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of Physics D Applied Physics

Cytotoxic effects of human epithelial carcinoma HeLa cells sensitivity to human mammary epithelial MCF10A cells appeared in incubation with the plasma-activated medium (PAM), where the cell culture media were irradiated with the hollow-shaped contact of a continuously discharged plasma that was sustained by application of a microwave power under Ar gas flow at atmospheric pressure. The discharged plasma had an electron density of 7

DOI： 10.1088/1361-6463/aaab09

Free radical generation by non-equilibrium atmospheric pressure plasma in alcohol-water mixtures: an EPR-spin trapping study 査読有り

Uchiyama, H; Ishikawa, K; Zhao, QL; Andocs, G; Nojima, N; Takeda, K; Krishna, MC; Ishijima, T; Matsuya, Y; Hori, M; Noguchi, K; Kondo, T

JOURNAL OF PHYSICS D-APPLIED PHYSICS 51 巻 ( 9 ) 2018年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of Physics D Applied Physics

Free radical species in aqueous solution - various alcohol-water reaction mixtures - by exposure to non-equilibrium cold atmospheric pressure Ar plasma (CAP), were monitored using electron paramagnetic resonance spin-trapping techniques with 3, 5-dibromo-4-nitrosobenzene sulfonate as a water soluble nitroso spin trap. The major radical species were formed by H-abstraction from alcohol molecules due to •OH radicals. In the ethanol-water mixture •CH<inf>2</inf>CH<inf>2</inf>OH produced by H abstraction from CH<inf>3</inf> group of the ethanol and •CH<inf>3</inf> radicals were detected. The latter was due to the decomposition of unstable CH<inf>3</inf>•CHOH to form the •CH<inf>3</inf> radicals and the stable formaldehyde by C-C bond fission. These intermediates are similar to those observed by reaction with •OH radicals generation in the H<inf>2</inf>O<inf>2</inf>-UV photolysis of the reaction mixtures. The evidence of •CH<inf>3</inf> radical formation in the pyrolytic decomposition of the reaction mixtures by exposure to ultrasound or in methane irradiated with microwave plasma have been reported previously. However, the pyrolytic •CH<inf>3</inf> radicals were not found in both plasma and H<inf>2</inf>O<inf>2</inf>-UV photolysis condition. These results suggests that free radicals produced by Ar-CAP are most likely due to the reaction between abundant •OH radicals and alcohol molecules.

DOI： 10.1088/1361-6463/aaa885

Facile fabrication of a poly(ethylene terephthalate) membrane filter with precise arrangement of through-holes 査読有り

Kihara, N; Odaka, H; Kuboyama, D; Onoshima, D; Ishikawa, K; Baba, Y; Hori, M

JAPANESE JOURNAL OF APPLIED PHYSICS 57 巻 ( 3 ) 2018年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Japanese Journal of Applied Physics

Although membrane filters are indispensable in biochemical analysis fields, most methods for through-hole fabrication are complex and inefficient. We developed a simple method of fabricating poly(ethylene terephthalate) (PET) membrane filters with a precise arrangement of through-holes for the isolation of circulating tumor cells (CTCs) based on their size. By photolithography and dry etching, highly packed 380,000 through-holes with a diameter of 7 μm were able to cover a whole area with a diameter of 13 mm. Device fabrication for the size-based capture of rare cells in blood such as CTCs is realized in this study.

DOI： 10.7567/JJAP.57.037001

Reduced HeLa cell viability in methionine-containing cell culture medium irradiated with microwave-excited atmospheric-pressure plasma 査読有り

Takahashi, Y; Taki, Y; Takeda, K; Hashizume, H; Tanaka, H; Ishikawa, K; Hori, M

PLASMA PROCESSES AND POLYMERS 15 巻 ( 3 ) 2018年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Plasma Processes and Polymers

The mechanism underlying the death of cancer cells incubated in plasma-activated medium (PAM) prepared by irradiation of culture medium with atmospheric-pressure plasma is revealed by analyzing changes in the culture medium composition using liquid chromatography-tandem mass spectrometry and nuclear magnetic resonance spectroscopy. The concentration of the essential amino acid methionine declined in microwave-excited plasma-irradiated medium, whereas, that of methionine sulfoxide increased due to oxidation of methionine. The decrease in methionine concentration in the cell culture medium rather than the increase in methionine sulfoxide leads to the decline in the survival rate of HeLa cells incubated in PAM. These results provide useful insights into the mechanism of selective cancer cell death in PAM.

DOI： 10.1002/ppap.201700200

Nanographene synthesis employing in-liquid plasmas with alcohols or hydrocarbons 査読有り

Ando, A; Ishikawa, K; Kondo, H; Tsutsumi, T; Takeda, K; Ohta, T; Ito, M; Hiramatsu, M; Sekine, M; Hori, M

JAPANESE JOURNAL OF APPLIED PHYSICS 57 巻 ( 2 ) 2018年2月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Japanese Journal of Applied Physics

Graphenes of nanometer-scale grain size (nanographenes) were synthesized using in-liquid plasmas with alcohols or hydrocarbons. This method of nanographene synthesis showed a trade-off relationship between crystallinity and synthesis rate. The high crystallinity of nanographenes synthesized with alcohols was evaluated from the small full width at half maxima (FWHM) of the G band in Raman scattering spectra. On the other hand, in the case of using hydrocarbons such as n-hexane and benzene, a significantly high synthesis rate was obtained but the crystallinity of nanographenes was low. It was found that hydroxyl groups and oxygen atoms of liquid sources play important roles in determining the crystallinity of synthesized nanographenes.

DOI： 10.7567/JJAP.57.026201

Advanced Plasma Science and Its Applications for Nitrides and Nanomaterials FOREWORD 査読有り Open Access

Naritsuka, S; Miyazaki, S; Fujiwara, Y; Hiramatsu, M; Inoue, Y; Ishikawa, K; Ito, M; Itoh, T; Kasu, M; Miyake, H; Sasaki, M; Shirafuji, T; Suda, Y

JAPANESE JOURNAL OF APPLIED PHYSICS 57 巻 ( 1 ) 2018年1月

Electron impact ionization of perfluoro-methyl-vinyl-ether C<sub>3</sub>F<sub>6</sub>O 査読有り

Kondo, Y; Ishikawa, K; Hayashi, T; Sekine, M; Hori, M

PLASMA SOURCES SCIENCE & TECHNOLOGY 27 巻 ( 1 ) 2018年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Plasma Sources Science and Technology

A mechanism for the electron impact ionization of perfluoro-methyl-vinyl-ether (CF<inf>3</inf>OCF=CF<inf>2</inf>) molecules was studied theoretically using quantum chemical calculations. Positive and negative C<inf>2</inf>F<inf>3</inf>O<sup>-</sup> ions were abundantly generated, as supported by quadrupole mass spectrometry observations. The vinyl ethers easily dissociated at the ether bond due to polarization of the charge on the bridging oxygen atom. Consequently, perfluoro-vinyl-ethers form abundant ions via ether-specific pathways.

DOI： 10.1088/1361-6595/aaa22e

Plasma-activated medium (PAM) kills human cancer-initiating cells 査読有り Open Access

Ikeda, J; Tanaka, H; Ishikawa, K; Sakakita, H; Ikehara, Y; Hori, M

PATHOLOGY INTERNATIONAL 68 巻 ( 1 ) 頁： 23 - 30 2018年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Pathology International

Medical non-thermal plasma (NTP) treatments for various types of cancers have been reported. Cells with tumorigenic potential (cancer-initiating cells; CICs) are few in number in many types of tumors. CICs efficiently eliminate anti-cancer chemicals and exhibit high-level aldehyde dehydrogenase (ALDH) activity. We previously examined the effects of direct irradiation via NTP on cancer cells; even though we targeted CICs expressing high levels of ALDH, such treatment affected both non-CICs and CICs. Recent studies have shown that plasma-activated medium (PAM) (culture medium irradiated by NTP) selectively induces apoptotic death of cancer but not normal cells. Therefore, we explored the anti-cancer effects of PAM on CICs among endometrioid carcinoma and gastric cancer cells. PAM reduced the viability of cells expressing both low and high levels of ALDH. Combined PAM/cisplatin appeared to kill cancer cells more efficiently than did PAM or cisplatin alone. In a mouse tumor xenograft model, PAM exerted an anti-cancer effect on CICs. Thus, our results suggest that PAM effectively kills both non-CICs and CICs, as does NTP. Therefore, PAM may be a useful new anti-cancer therapy, targeting various cancer cells including CICs.

DOI： 10.1111/pin.12617

Selective production of reactive oxygen and nitrogen species in the plasma-treated water by using a nonthermal high-frequency plasma jet 査読有り

Uchida, G; Takenaka, K; Takeda, K; Ishikawa, K; Hori, M; Setsuhara, Y

JAPANESE JOURNAL OF APPLIED PHYSICS 57 巻 ( 1 ) 2018年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Japanese Journal of Applied Physics

We present the control of H<inf>2</inf>O<inf>2</inf> and NO<inf>2</inf> <sup>-</sup> productions in deionized water by using a high-frequency plasma jet driven by a 60 MHz voltage. In the gas phase, the high-frequency plasma jet has a high O (<sup>3</sup>P) atom density of 8 × 10<sup>14</sup> cm<sup>-3</sup>, which is two orders of magnitude higher than that of the low-frequency plasma jet driven by a 5 kHz voltage. Concerning the production of reactive oxygen and nitrogen species in the liquid phase, with the direct contact of the plasma jet to the liquid surface, the H<inf>2</inf>O<inf>2</inf> concentration is higher than the NO<inf>2</inf> <sup>-</sup> concentration. On the other hand, without the observable contact of the high-frequency plasm jet with high plasma density to the liquid surface, the NO<inf>2</inf> <sup>-</sup> concentration increases with the flow rate of N<inf>2</inf>(20%)O<inf>2</inf>(80%) gas added to the Ar discharge gas and becomes more dominant compared with H<inf>2</inf>O<inf>2</inf> in the plasma-treated water. H<inf>2</inf>O<inf>2</inf> and NO<inf>2</inf> <sup>-</sup> could be selectively produced in the plasma-treated water by using a nonthermal high-frequency plasma jet, which is a promising tool for biomedical applications.

DOI： 10.7567/JJAP.57.0102B4

Rapid growth of micron-sized graphene flakes using in-liquid plasma employing iron phthalocyanine-added ethanol 査読有り Open Access

Amano, T; Kondo, H; Ishikawa, K; Tsutsumi, T; Takeda, K; Hiramatsu, M; Sekine, M; Hori, M

APPLIED PHYSICS EXPRESS 11 巻 ( 1 ) 2018年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Applied Physics Express

Giant graphene flakes on the micron scale were synthesized and grown in plasmas in liquid-phase pure ethanol with added iron phthalocyanine (FePc) in a solvent. At atmospheric pressure, plasmas were generated in the gas phase filled with Ar and in the liquid phases comprising bubbles and liquid solutions. In the mixture of FePc in ethanol, nanographene sheets aggregated to form giant graphene flakes, as confirmed by the D, G, and 2D bands in the corresponding Raman spectra. Therefore, a bottom-up approach of graphite synthesis from pure ethanol with additives and a catalyst was realized by in-liquid plasma processing.

DOI： 10.7567/APEX.11.015102

Cell Deposition Microchip with Micropipette Control over Liquid Interface Motion. 査読有り

Onoshima D, Hattori Y, Yukawa H, Ishikawa K, Hori M, Baba Y

Cell medicine 10 巻頁： 2155179017733152 2018年

Isotropic atomic level etching of tungsten using formation and desorption of tungsten fluoride 査読有り

Shinoda, K; Miyoshi, N; Kobayashi, H; Hanaoka, Y; Kawamura, K; Izawa, M; Ishikawa, K; Hori, M

ADVANCED ETCH TECHNOLOGY FOR NANOPATTERNING VII 10589 巻 2018年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Proceedings of SPIE the International Society for Optical Engineering

A selective, rapid thermal-cyclic atomic-level etching (ALE) of tungsten is developed. The first step of this process is exposing the surface of tungsten with hydrofluorocarbon plasma at -22°C to form a tungsten fluoride-based surface modified layer on the tungsten surface. The second step is rapid thermal annealing with infrared (IR) irradiation to remove the surface modified layer. Tungsten 4f peaks and a fluorine 1s peak, which were assigned to tungsten fluoride, were observed by in-situ x-ray photoelectron spectroscopy immediately after plasma exposure. The peaks that originated from tungsten fluoride disappeared after the samples were annealed. Cyclic etching tests were carried out by repeating plasma exposure and IR irradiation with a 300-mm ALE tool. Films of tungsten, TiN, and SiO<inf>2</inf> were used as sample materials. The amount of etched tungsten increased as the number of cycle repetitions increased. The etched amount per cycle for tungsten was 0.8 nm. In comparison, etching of TiN and SiO<inf>2</inf> was not detected. Conformal etching profiles of patterned samples after 60 cycles were obtained. Furthermore, the etched amount per cycle showed saturation behavior with regard to plasma exposure time. Selective, rapid thermal cyclic ALE of tungsten was thus successfully demonstrated.

DOI： 10.1117/12.2297241

Glioblastoma Cell Lines Display Different Sensitivities to Plasma-Activated Medium 査読有り

Tanaka H., Mizuno M., Ishikawa K., Takeda K., Hashizume H., Nakamura K., Utsumi F., Kajiyama H., Okazaki Y., Toyokuni S., Akiyama S., Maruyama S., Kikkawa F., Hori M.

IEEE Transactions on Radiation and Plasma Medical Sciences 2 巻 ( 2 ) 頁： 99 - 102 2018年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：IEEE Transactions on Radiation and Plasma Medical Sciences

Plasma-activated medium (PAM) is a novel chemotherapy that induces reactive oxygen species (ROS) and cell death in a wide range of cancer cell types, suggesting that PAM may be a promising therapeutic option for cancer treatment. However, dose response experiments suggest that PAM sensitivity is cell line specific. We examined the sensitivities of three glioblastoma cell lines to PAM, and found a wide variation in cell killing that was linked to differences in PAM induced ROS and apoptosis. These results indicate that the PAM sensitivity of glioblastoma cells, and potentially cancer cells more generally, is heterogeneous and likely to be dependent on the regulation of apoptosis and antioxidant pathways in target cells.

DOI： 10.1109/trpms.2017.2721973

Oncogene mutation analysis of circulating tumor cells using single-cell membrane separation and DNA amplification 査読有り

Onoshima D., Kuboyama D., Kihara N., Tanaka H., Hase T., Yukawa H., Ishikawa K., Odaka H., Hasegawa Y., Hori M., Baba Y.

22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences Microtas 2018 3 巻頁： 1291 - 1293 2018年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences Microtas 2018

We present a study of genomic profiling for circulating tumor cells (CTCs) using a blood microfiltration system. DNA amplification of CTCs was performed with a microfilter for single cancer cells in a clinical setting. This strategy was successfully used to detect the driver mutations in epidermal growth factor (EGF) receptor oncogenes by quantitative PCR and characterize the dosing effect of molecularly targeted drug for lung cancer.

Exosome isolation toward cancer diagnosis using glass filter with nanoporous structure 査読有り Open Access

Aoki K., Yukawa H., Onoshima D., Yamazaki S., Kihara N., Koguchi R., Takahashi K., Odaka H., Ishikawa K., Hori M., Baba Y.

22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences Microtas 2018 3 巻頁： 1409 - 1410 2018年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences Microtas 2018

We present a study of centrifugal filtration device toward easy-to-use approach of non-invasive liquid biopsy for cancer diagnosis by cell-derived nanoscale vesicles (exosomes). A phase-dispersed glass filter having nanoporous structure was embedded into a spin column to trap exosomes with small centrifuge. This device enabled over 90% exosome isolation from biological samples within 10 minutes.

DOI： 10.1088/1361-6595/ac12bf

Dynamic analysis of reactive oxygen nitrogen species in plasma-activated culture medium by UV absorption spectroscopy 査読有り国際共著

Brubaker, TR; Ishikawa, K; Takeda, K; Oh, JS; Kondo, H; Hashizume, H; Tanaka, H; Knecht, SD; Bilén, SG; Hori, M

JOURNAL OF APPLIED PHYSICS 122 巻 ( 21 ) 2017年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of Applied Physics

The liquid-phase chemical kinetics of a cell culture basal medium during treatment by an argon-fed, non-equilibrium atmospheric-pressure plasma source were investigated using real-time ultraviolet absorption spectroscopy and colorimetric assays. Depth- and time-resolved NO2- and NO3- concentrations were strongly inhomogeneous and primarily driven by convection during and after plasma-liquid interactions. H<inf>2</inf>O<inf>2</inf> concentrations determined from deconvolved optical depth spectra were found to compensate for the optical depth spectra of excluded reactive species and changes in dissolved gas content. Plasma-activated media remained weakly basic due to NaHCO<inf>3</inf> buffering, preventing the H<sup>+</sup>-catalyzed decomposition of NO2- seen in acidic plasma-activated water. An initial increase in pH may indicate CO<inf>2</inf> sparging. Furthermore, the pH-dependency of UV optical depth spectra illustrated the need for pH compensation in the fitting of optical depth data.

DOI： 10.1063/1.4999256

Dependence of absolute photon flux on infrared absorbance alteration and surface roughness on photoresist polymers irradiated with vacuum ultraviolet photons emitted from HBr plasma 査読有り

Zhang, Y; Takeuchi, T; Ishikawa, K; Hayashi, T; Takeda, K; Sekine, M; Hori, M

JAPANESE JOURNAL OF APPLIED PHYSICS 56 巻 ( 12 ) 2017年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Japanese Journal of Applied Physics

The absolute fluxes of vacuum ultraviolet (VUV) photons emitted from HBr plasma were analyzed and the effects of VUV photons on a photoresist polymer in ArF-excimer-laser (193 nm) lithography were quantitatively investigated on the basis of the infrared spectra attributed to the C=O region. The spectral peak intensity assigned to the methacrylic acid (MAA) in the photoresist drastically decreased owing to the loss of this monomer caused by the irradiation of VUV photons at dosages below 16 - 10<sup>16</sup> photons/cm<sup>2</sup>. X-ray photoelectron spectroscopy observation showed that the removed monomer moved to the surface and generated volatile products that induced a decrease in film thickness. As a consequence, the surface became rough during the early-stage irradiation at dosages lower than 16 - 10<sup>16</sup> photons/cm<sup>2</sup> owing to the monomer loss of MAA with volatile product formation and subsequent cross-linking reactions.

DOI： 10.7567/JJAP.56.126503

State of the art in medical applications using non-thermal atmospheric pressure plasma 査読有り国際共著

Tanaka H., Ishikawa K., Mizuno M., Toyokuni S., Kajiyama H., Kikkawa F., Metelmann H.R., Hori M.

Reviews of Modern Plasma Physics 1 巻 ( 1 ) 2017年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Reviews of Modern Plasma Physics

Plasma medical science is a novel interdisciplinary field that combines studies on plasma science and medical science, with the anticipation that understanding the scientific principles governing plasma medical science will lead to innovations in the field. Non-thermal atmospheric pressure plasma has been used for medical treatments, such as for cancer, blood coagulation, and wound healing. The interactions that occur between plasma and cells/tissues have been analyzed extensively. Direct and indirect treatment of cells with plasma has broadened the applications of non-thermal atmospheric pressure plasma in medicine. Examples of indirect treatment include plasma-assisted immune-therapy and plasma-activated medium. Controlling intracellular redox balance may be key in plasma cancer treatment. Animal studies are required to test the effectiveness and safety of these treatments for future clinical applications.

DOI： 10.1007/s41614-017-0004-3

Intracellular responses to reactive oxygen and nitrogen species, and lipid peroxidation in apoptotic cells cultivated in plasma-activated medium 査読有り

Furuta, R; Kurake, N; Ishikawa, K; Takeda, K; Hashizume, H; Tanaka, H; Kondo, H; Sekine, M; Hori, M

PLASMA PROCESSES AND POLYMERS 14 巻 ( 11 ) 2017年11月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Plasma Processes and Polymers

A mechanism of the cytotoxicity of plasma-activated medium (PAM) is revealed by examining the intracellular effects of reactive oxygen nitrogen species (RONS) and lipid oxidation. PAM is cell culture medium activated by irradiation using non-equilibrium atmospheric-pressure plasma using pure Ar gas in ambient air. Extracellular RONS in PAM induced the apoptotic death of HeLa cells. Temporal changes in intracellular RONS, such as ONOO<sup>−</sup>, NO, and (Formula presented.), were analyzed. Intracellular RONS generation in HeLa cells following incubation in PAM triggered activation of the caspase cascade pathway and lipid peroxidation of intracellular membranes to induce apoptosis.

DOI： 10.1002/ppap.201700123

Surface roughening of photoresist after change of the photon/radical and ion treatment sequence 査読有り

Zhang, Y; Takeuchi, T; Ishikawa, K; Takeda, K; Kondo, H; Sekine, M; Hori, M

JOURNAL OF VACUUM SCIENCE & TECHNOLOGY A 35 巻 ( 6 ) 2017年11月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of Vacuum Science and Technology A Vacuum Surfaces and Films

The formation of surface roughness during ion irradiation was suppressed by photon/radical pretreatment of a photoresist with HBr or H<inf>2</inf> plasmas. The opposite sequence of treatment after ion irradiation enhanced the surface roughness. The enhancement of roughness after post-treatment was not correlated with a decrease in the film thickness, reactions of Br radicals at the surface, or with the presence of the amorphous carbon-like layer generated by ion irradiation because similar modification was observed for the pretreatment. Fourier transform infrared spectroscopy analysis revealed that the intensities of peaks originated from the C=O absorption of methacrylic acid and methacrylate groups were decreased by irradiation with vacuum ultraviolet light at wavelengths in the range between 115 and 170 nm emitted from HBr or H<inf>2</inf> plasmas. The removal of the C=O groups possibly generated dangling bonds and induced crosslinking reactions of the photoresist polymers. The nonuniformity of crosslinking reactions on the roughened photoresist intensively increased the surface roughness.

DOI： 10.1116/1.4994218

　前のページ - 次のページ　

1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22