研究者詳細 - 豊國　伸哉

論文 - 豊國　伸哉

分割表示 1,236 件中 141 - 160 件目／全件表示 >>

Preclinical Verification of the Efficacy and Safety of Aqueous Plasma for Ovarian Cancer Therapy. 国際誌 Open Access

Nakamura K, Yoshikawa N, Mizuno Y, Ito M, Tanaka H, Mizuno M, Toyokuni S, Hori M, Kikkawa F, Kajiyama H

Cancers 13 巻 ( 5 ) 頁： 1 - 15 2021年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Cancers

Epithelial ovarian cancer (EOC) is the most lethal gynecologic malignancy. The major cause of EOC’s lethality is that intraperitoneal recurrence occurs with high frequency due to occult metastasis. We had demonstrated that plasma-activated medium (PAM) exerts a metastasis-inhibitory effect on ovarian cancer in vitro and in vivo. Here we investigated how PAM inhibits intraperitoneal metastasis. We studied PAM’s inhibition of micro-dissemination onto the omentum by performing in vivo imaging in combination with a sequential histological analysis. The results revealed that PAM induced macrophage infiltration into the disseminated lesion. The iNOS-positive signal was co-localized at the macrophages in the existing lesion, indicating that PAM might induce M1-type macrophages. This may be another mechanism of the antitumor effect through a PAM-evoked immune response. Intraperitoneal lavage with plasma-activated lactate Ringer’s solution (PAL) significantly improved the overall survival rate in an ovarian cancer mouse model. Our results demonstrated the efficiency and practicality of aqueous plasma for clinical applications.

DOI： 10.3390/cancers13051141

Role of ferroptosis in carcinogenesis and tumor biology

Toyokuni Shinya

FREE RADICAL BIOLOGY AND MEDICINE 165 巻 2021年3月

Mechanism of asbestos-induced carcinogenesis via dysregulation of redox-active iron 国際誌

Ito Fumiya, Toyokuni Shinya

CANCER SCIENCE 112 巻頁： 998 - 998 2021年2月

Mechanism of asbestos-induced carcinogenesis via dysregulation of redox-active iron 国際誌

Ito Fumiya, Toyokuni Shinya

CANCER SCIENCE 112 巻頁： 998 - 998 2021年2月

Signature analysis of genomic mutations in asbestos-induced rat mesothelioma

Akatsuka Shinya, Jiang Li, Elzawahry Asmaa, Kato Mamoru, Totsuka Yukari, Shibata Tatsuhiro, Toyokuni Shinya

CANCER SCIENCE 112 巻頁： 610 - 610 2021年2月

Asbestos and talc contribute to ovarian carcinogenesis via iron overload

Motooka Yashiro, Ito Fumiya, Tashiro Hironori, Katabuchi Hidetaka, Toyokuni Shinya

CANCER SCIENCE 112 巻頁： 245 - 245 2021年2月

Role of redox-active metals for the prevention and treatment of cancer in the era of precision medicine

Toyokuni Shinya, Richardson Des R.

CANCER SCIENCE 112 巻頁： 996 - 996 2021年2月

Defective biosynthesis of ascorbic acid in Sod1-deficient mice results in lethal damage to lung tissue 国際誌

Homma T.

Free Radical Biology and Medicine 162 巻頁： 255 - 265 2021年1月

Prognostic significance of the MDM2/HMGA2 ratio and histological tumor grade in dedifferentiated liposarcoma 国際誌

Yamashita K.

Genes Chromosomes and Cancer 60 巻 ( 1 ) 頁： 26 - 37 2021年1月

Cancer Treatments Using Low-Temperature Plasma. 査読有り国際誌

Tanaka H, Mizuno M, Ishikawa K, Toyokuni S, Kajiyama H, Kikkawa F, Hori M

Current medicinal chemistry 28 巻 ( 41 ) 頁： 8549 - 8558 2021年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Current Medicinal Chemistry

Low-temperature plasma (LTP) is a partially ionized gas that contains elec-trons, ions, radicals, light, etc. Recently, the bio-medical application of LTP has become a hot topic in plasma science and biological science. Cancer treatment with plasma is the most challenging topic in plasma bio-medical applications. Many in vitro and in vivo ex-periments have been conducted to investigate the anti-tumor effects of LTP. Extracellular reactive oxygen and nitrogen species (RONS) in plasma-activated solutions are key factors for the anti-tumor effects, and amino acid modifications by LTP may affect cellular responses. Intracellular RONS are also key factors for the anti-tumor effects. Various signaling pathways, such as p53 signaling pathways, survival and proliferation signaling pathways, and oxidative stress-dependent signaling pathways are activated by LTP.

DOI： 10.2174/0929867328666210629121731

山口慎一朗, 守田匡伸, 伊藤文哉, 謝祺琳, 豊國伸哉, 中山勝文

日本毒性学会学術年会 48.1 巻 ( 0 ) 頁： O-4 - 4 2021年

記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：日本毒性学会

カーボンナノチューブ（CNTs）は次世代ナノ材料として期待されているものの、その毒性の懸念から国際化学物質事務局ChemSecがCNTsをSIN (Substitute It Now)リストに加えたため、今後のCNTsの使用継続について国際的に大きく議論されている。多層CNTs（MWCNTs）は生体内に入ると主にマクロファージに取り込まれ、そのマクロファージ細胞死およびNLRP3インフラマソーム活性化によるIL-1β分泌が毒性発現に関与すると考えられているが、マクロファージがどのように細胞表面上でMWCNTsを認識するのかはよく分かっていない。本研究で我々は貪食受容体スクリーニングによりT cell immunoglobulin mucin 4（Tim4）およびTim1がMWCNTsを認識することを見出した。CRISPR/Cas9システムによって作製したTim4-/- Tim1-/- マウスやTim4-/- マウス由来腹腔マクロファージを用いた解析から、Tim4はマクロファージによるMWCNTsの貪食、およびその結果起きるIL-1β分泌に関与することが判明した。さらにTim4-/- マウス、あるいは抗Tim4モノクローナル抗体を全投与した野生型マウスにおいて、MWCNTs腹腔投与による横隔膜中皮細胞層の肉芽種形成が有意に抑制されていたことから、Tim4はMWCNTs曝露によるin vivo炎症応答に関与することが示唆された。一方、Tim1はヒト肺細胞株によるMWCNTsの認識に関与することが判明した。以上の結果は、Tim4およびTim1はMWCNTs受容体として機能することを示す。

DOI： 10.14869/toxpt.48.1.0_o-4

CiNii Research

The new era for research on polyphenols and food factors 国際誌

Oteiza P.I.

Archives of Biochemistry and Biophysics 696 巻 2020年12月

The new era for redox research. 国際誌 Open Access

Naito Y, Uchida K, Toyokuni S

Free radical research 54 巻 ( 11-12 ) 頁： 787 - 789 2020年12月

Neural stem cell-specific ITPA deficiency causes neural depolarization and epilepsy. 国際誌 Open Access

Koga Y, Tsuchimoto D, Hayashi Y, Abolhassani N, Yoneshima Y, Sakumi K, Nakanishi H, Toyokuni S, Nakabeppu Y

JCI insight 5 巻 ( 22 ) 2020年11月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：JCI Insight

Inosine triphosphate pyrophosphatase (ITPA) hydrolyzes inosine triphosphate (ITP) and other deaminated purine nucleotides to the corresponding nucleoside monophosphates. In humans, ITPA deficiency causes severe encephalopathy with epileptic seizure, microcephaly, and developmental retardation. In this study, we established neural stem cell–specific Itpa–conditional KO mice (ItpacKO mice) to clarify the effects of ITPA deficiency on the neural system. The Itpa-cKO mice showed growth retardation and died within 3 weeks of birth. We did not observe any microcephaly in the Itpa-cKO mice, although the female Itpa-cKO mice did show adrenal hypoplasia. The Itpa-cKO mice showed limb-clasping upon tail suspension and spontaneous and/or audiogenic seizure. Whole-cell patch-clamp recordings from entorhinal cortex neurons in brain slices revealed a depolarized resting membrane potential, increased firing, and frequent spontaneous miniature excitatory postsynaptic current and miniature inhibitory postsynaptic current in the Itpa-cKO mice compared with ITPA-proficient controls. Accumulated ITP or its metabolites, such as cyclic inosine monophosphates, or RNA containing inosines may cause membrane depolarization and hyperexcitability in neurons and induce the phenotype of ITPA-deficient mice, including seizure.

DOI： 10.1172/jci.insight.140229

Carcinogenesis as Side Effects of Iron and Oxygen Utilization: From the Unveiled Truth toward Ultimate Bioengineering. 国際誌 Open Access

Toyokuni S, Kong Y, Cheng Z, Sato K, Hayashi S, Ito F, Jiang L, Yanatori I, Okazaki Y, Akatsuka S

Cancers 12 巻 ( 11 ) 頁： 1 - 20 2020年11月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Cancers

Evolution from the first life on earth to humans took ~3.8 billion years. During the time there have been countless struggles among the species. Mycobacterium tuberculosis was the last major uncontrollable species against the human public health worldwide. After the victory with antibiotics, cancer has become the leading cause of death since 1981 in Japan. Considering that life inevitably depends on ceaseless electron transfers through iron and oxygen, we believe that carcinogenesis is intrinsically unavoidable side effects of using iron and oxygen. Many animal models unequivocally revealed that excess iron is a risk for carcinogenesis. This is supported by a variety of human epidemiological data on cancer risk and prognosis. Cancer is basically a disease of the genome with persistently activated oncogenes and inactivated tumor suppressor genes through which iron addiction with ferroptosis-resistance is maintained. Engineering has made a great advance in the past 50 years. In particular, nanotechnology is distinct in that the size of the engineered molecules is similar to that of our biomolecules. While some nano-molecules are found carcinogenic, there are principles to avoid such carcinogenicity with a smart possibility to use nano-molecules to specifically kill cancer cells. Non-thermal plasma is another modality to fight against cancer.

DOI： 10.3390/cancers12113320

Induction of cancer cell-specific ferroptosis by non-thermal plasma exposure

Okazaki Yasumasa, Toyokuni Shinya

JAPANESE JOURNAL OF APPLIED PHYSICS 59 巻 ( 11 ) 2020年11月