研究者詳細 - 豊國　伸哉

論文 - 豊國　伸哉

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Production of knockout mice by gene targeting in multipotent germline stem cells. 査読有り

Takehashi M, Kanatsu-Shinohara M, Miki H, Lee J, Kazuki Y, Inoue K, Ogonuki N, Toyokuni S, Oshimura M, Ogura A and Shinohara T.

Dev. Biol. 312 巻頁： 344-352 2007年

Visualization and characterization of UVB-induced reactive oxygen species in a human skin equivalent model. 査読有り

Hakozaki T, Date A, Yoshii T, Toyokuni S, Yasui H and Sakurai H.

J. Dermatol. Res. 300 (Supplement 1) 巻頁： S51-56 2007年

Analysis of genes associated with karyo-megalic cells in rat kidney after ferric nitilotriacetate treatment 国際誌

Lee, WH; Toyokuni, S

FREE RADICAL BIOLOGY AND MEDICINE 43 巻頁： S24 - S24 2007年

ARMET is a soluble ER protein induced by the unfolded protein response via ERSE-II element.

Mizobuchi N, Hoseki J, Kubota H, Toyokuni S, Nozaki J, Naitoh M, Koizumi A, Nagata K

Cell structure and function 32 巻 ( 1 ) 頁： 41 - 50 2007年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Cell Structure and Function

Arginine rich, mutated in early stage of tumors (ARMET) was first identified as a human gene highly mutated in a variety of cancers. However, little is known about the characteristics of the ARMET protein and its expression. We identified ARMET as a gene upregulated by endoplasmic reticulum (ER) stress. Here, we show that the mouse homologue of ARMET is an 18-kDa soluble ER protein that is mature after cleavage of a signal sequence and has four intramolecular disulfide bonds, including two in CXXC sequences. ER stress stimulated ARMET expression, and the expression patterns of ARMET mRNA and protein in mouse tissues were similar to those of Grp78, an Hsp70-family protein required for quality control of proteins in the ER. A reporter gene assay using a mouse ARMET promoter revealed that the unfolded protein response of the ARMET gene is regulated by an ERSE-II element whose sequence is identical to that of the HERP gene. ARMET is the second fully characterized ERSE-II-dependent gene and likely contributes to quality control of proteins in the ER. © 2007 by Japan Society for Cell Biology.

DOI： 10.1247/csf.07001

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Chronic oxidative stress causes amplification and overexpresson of ptprzl protein tyrosine phosphatase to activate beta-catenin pathway

Liu, Y; Toyokuni, S

FREE RADICAL BIOLOGY AND MEDICINE 43 巻頁： S154 - S155 2007年

Visualization and characterization of UVB-induced reactive oxygen species in a human skin equivalent model. 査読有り

Hakozaki T, Date A, Yoshii T, Toyokuni S, Yasui H, Sakurai H

J. Dermatol. Res. 300 (Supplement 1) 巻頁： S51-56 2007年

Oxidative stress is related to formation of Antoni B pattern and eosinophilic hyaline droplets in schwannoma. 査読有り

Yokoo H, Oishi T, Isoda, K, Nakazato Y, Toyokuni S

Neuropathology 27 巻頁： 237-244 2007年

ARMET is a soluble ER protein induced by the unfolded protein response via ERSE-II element 査読有り

Naomi Mizobuchi, Jun Hoseki, Hiroshi Kubota, Shinya Toyokuni, Jun-ichi Nozaki, Motoko Naitoh, Akio Koizumi, Kazuhiro Nagata

CELL STRUCTURE AND FUNCTION 32 巻 ( 1 ) 頁： 41 - 50 2007年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：JAPAN SOC CELL BIOLOGY

Arginine rich, mutated in early stage of tumors ( ARMET) was first identified as a human gene highly mutated in a variety of cancers. However, little is known about the characteristics of the ARMET protein and its expression. We identified ARMET as a gene upregulated by endoplasmic reticulum ( ER) stress. Here, we show that the mouse homologue of ARMET is an 18- kDa soluble ER protein that is mature after cleavage of a signal sequence and has four intramolecular disulfide bonds, including two in CXXC sequences. ER stress stimulated ARMET expression, and the expression patterns of ARMET mRNA and protein in mouse tissues were similar to those of Grp78, an Hsp70- family protein required for quality control of proteins in the ER. A reporter gene assay using a mouse ARMET promoter revealed that the unfolded protein response of the ARMET gene is regulated by an ERSE- II element whose sequence is identical to that of the HERP gene. ARMET is the second fully characterized ERSE- II- dependent gene and likely contributes to quality control of proteins in the ER.

DOI： 10.1247/csf.07001

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Angiotensin II, oxidative stress, and extracellular matrix degradation during transition to LV failure in rats with hypertension. 査読有り

Takenaka H, Kihara Y, Iwanaga Y, Onozawa Y, Toyokuni S, Kita T

J. Mol. Cell. Cardiol. 41 巻頁： 989-997 - 997 2007年

ARMET is a soluble ER protein induced by the unfolded protein response via ERSE-II element

Mizobuchi Naomi, Hoseki Jun, Kubota Hiroshi, Toyokuni Shinya, Nozaki Jun-ichi, Naitoh Motoko, Koizumi Akio, Nagata Kazuhiro

CELL STRUCTURE AND FUNCTION 32 巻 ( 1 ) 頁： 41 - 50 2007年

Novel screening method for ultraviolet protection : Combination of a human skin-equivalent model and 8-hydroxy-2'-deoxyguanosine

TOYOKUNI Shinya, YASUI Hiroyuki, DATE Akira, HAKOZAKI Tomohiro, AKATSUKA Shinya, KOHDA Haruyasu, YOSHII Takashi, SAKURAI Hiromu

Pathology international 56 巻 ( 12 ) 頁： 760 - 762 2006年12月

Angiotensin II, oxidative stress, and extracellular matrix degradation during transition to LV failure in rats with hypertension. 査読有り国際誌

Takenaka H, Kihara Y, Iwanaga Y, Onozawa Y, Toyokuni S, Kita T

Journal of molecular and cellular cardiology 41 巻 ( 6 ) 頁： 989 - 97 2006年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of Molecular and Cellular Cardiology

Angiotensin II (Ang-II) plays pivotal roles in the progression of left ventricular (LV) remodeling in diseased hearts; it remains to be elucidated how Ang-II links to degradation of the extracellular matrix (ECM). Using hypertensive Dahl salt-sensitive rats that show the distinctive transition from concentric LV hypertrophy to LV remodeling, we chronically treated them with an angiotensin type-1 receptor blocker (telmisartan 5 mg/kg/day, ARB group) or vehicle (0.5% CMC, CHF group). During the process of LV remodeling, we assessed, (1) in-vivo LV shape and function; (2) animal survival; (3) amounts of ECM in LV using a scanning electron microscope (SEM); (4) mRNA (by real time RT-PCR) and protein (by immunoblotting) levels in LV of NADPH oxidase, glutathione peroxidase-1 (GPX-1), and matrix metalloproteinase (MMP)-2, -9, and -13; (5) immunohistochemical staining of myocardial 4-hydroxy-2-nonenal and 8-hydroxy-2′-deoxyguanosine; (6) nuclear factor kappa-B (NFκB) protein levels in the nuclear extract; and (7) endogenous activities of MMP-2 and -9 by an antibody capture method. Compared with CHF, ARB group showed an improvement of survival and preserved LV shape and function, and ECM density in SEM that was accompanied by decreases in oxidative stress-mediated protein degenerations, activities of GPX-1, NADPH oxidase, NFκB, and MMP-2, -9, and -13. Local activation of Ang-II in hypertrophic LV triggers MMP-mediated ECM degradation, namely LV remodeling, at least in part, through NADPH oxidase-induced oxidative stress and the subsequent NFκB activation. © 2006 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.yjmcc.2006.07.019

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Novel screening method for ultraviolet protection:: Combination of a human skin-equivalent model and 8-hydroxy-2′-deoxyguanosine 査読有り国際誌

Toyokuni, S; Yasui, H; Date, A; Hakozaki, T; Akatsuka, S; Kohda, H; Yoshii, T; Sakurai, H

PATHOLOGY INTERNATIONAL 56 巻 ( 12 ) 頁： 760 - 762 2006年12月

Deletion and single nucleotide substitution at G:C in the kidney of gpt delta transgenic mice after ferric nitrilotriacetate treatment

JIANG Li, ZHONG Yi, AKATSUKA Shinya, LIU Yu-Ting, DUTTA Khokon Kumar, LEE Wen-Hua, ONUKI Janice, MASUMURA Ken-ichi, NOHMI Takehiko, TOYOKUNI Shinya

Cancer science 97 巻 ( 11 ) 頁： 1159 - 1167 2006年11月

Deletion and single nucleotide substitution at G:C in the kidney of gpt delta transgenic mice after ferric nitrilotriacetate treatment. 査読有り国際誌 Open Access

Jiang L, Zhong Y, Akatsuka S, Liu YT, Dutta KK, Lee WH, Onuki J, Masumura K, Nohmi T, Toyokuni S

Cancer science 97 巻 ( 11 ) 頁： 1159 - 67 2006年11月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Cancer Science

An iron chelate, ferric nitrilotriacetate (Fe-NTA), induces oxidative renal proximal tubular damage that subsequently leads to a high incidence of renal cell carcinoma in rodents, presenting an intriguing model of free radical-induced carcinogenesis. In the present study, we used gpt delta transgenic mice, which allow efficient detection of point mutations and deletions in vivo, to evaluate the mutation spectra, in association with the formation of 8-oxoguanine and acrolein-modified adenine during the first 3 weeks of carcinogenesis. Immunohistochemical analysis revealed the highest levels of 8-oxoguanine and acrolein-modifed adenine in the renal proximal tubules after 1 week of repeated administration. DNA immunoprecipitation and quantitative polymerase chain reaction analysis showed that the relative abundance of 8-oxoguanine and acrolein-modified adenine at the gpt reporter gene were increased at the first week in the kidney. Similarly, in both 6-thioguanine and Spi- selections performed on the renal specimens after Fe-NTA administration, the mutant frequencies were increased in the Fe-NTA-treated mice at the first week. Further analyzes of 79 mutant clones and 93 positive plaques showed a high frequency of G:C pairs as preferred targets for point mutation, notably G:C to C:G transversion-type mutation followed by deletion, and of large-size (>1 kilobase) deletions with short homologous sequences in proximity to repeated sequences at the junctions. The results demonstrate that the iron-based Fenton reaction is mutagenic in vivo in the renal tubular cells and induces characteristic mutations. © 2006 Japanese Cancer Association.

DOI： 10.1111/j.1349-7006.2006.00301.x

Contrasting genome-wide distribution of 8-hydroxyguanine and acrolein-modified adenine during oxidative stress-induced renal carcinogenesis. 査読有り国際誌 Open Access

Akatsuka S, Aung TT, Dutta KK, Jiang L, Lee WH, Liu YT, Onuki J, Shirase T, Yamasaki K, Ochi H, Naito Y, Yoshikawa T, Kasai H, Tominaga Y, Sakumi K, Nakabeppu Y, Kawai Y, Uchida K, Yamasaki A, Tsuruyama T, Yamada Y, Toyokuni S

The American journal of pathology 169 巻 ( 4 ) 頁： 1328 - 42 2006年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：American Journal of Pathology

Oxidative stress is a persistent threat to the genome and is associated with major causes of human mortality, including cancer, atherosclerosis, and aging. Here we established a method to generate libraries of genomic DNA fragments containing oxidatively modified bases by using specific monoclonal antibodies to immunoprecipitate enzyme-digested genome DNA. We applied this technique to two different base modifications, 8-hydroxyguanine and 1,N 6-propanoadenine (acrotein-Ade), in a ferric nitrilotriacetate- induced murine renal carcinogenesis model. Renal cortical genomic DNA derived from 10- to 12-week-old male C57BL/6 mice, of untreated control or 6 hours after intraperitoneal injection of 3 mg iron/kg ferric nitrilotriacetate, was enzyme digested, immunoprecipitated, cloned, and mapped to each chromosome. The results revealed that distribution of the two modified bases was not random but differed in terms of chromosomes, gene size, and expression, which could be partially explained by chromosomal territory. In the wild-type mice, low GC content areas were more likely to harbor the two modified bases. Knockout of OGG1, a repair enzyme for genomic 8-hydroxyguanine, increased the amounts of acrolein-Ade as determined by quantitative polymerase chain reaction analyses. This versatile technique would introduce a novel research area as a high-throughput screening method for critical genomic loci under oxidative stress. Copyright © American Society for Investigative Pathology.

DOI： 10.2353/ajpath.2006.051280

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PubMed

Alpha-tocopherol induces calnexin in renal tubular cells: another protective mechanism against free radical-induced cellular damage. 査読有り国際誌 Open Access

Lee WH, Akatsuka S, Shirase T, Dutta KK, Jiang L, Liu YT, Onuki J, Yamada Y, Okawa K, Wada Y, Watanabe A, Kohro T, Noguchi N, Toyokuni S

Archives of biochemistry and biophysics 453 巻 ( 2 ) 頁： 168 - 78 2006年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Archives of Biochemistry and Biophysics

Pre-administration of α-tocopherol is protective against oxidative renal tubular damage and subsequent carcinogenesis by ferric nitrilotriacetate (Fe-NTA) in rats. We searched for mechanisms other than the scavenging effect of α-tocopherol with microarray analyses, which implicated calnexin, a chaperone for glycoproteins. Renal mRNA levels of calnexin significantly increased 3 h after an injection of Fe-NTA in rats fed a standard diet whereas those fed an α-tocopherol-supplemented diet showed an increase prior to injection, but after injection showed a decrease in renal calnexin mRNA levels, with unaltered protein levels. In experiments using LLC-PK1 cells, addition of α-tocopherol was protective against oxidative stress by H2O2, concomitant with calnexin induction. Knockdown of calnexin by siRNA significantly reduced this protection. Furthermore, COS-7 cells transfected with the calnexin gene were more resistant to H2O2. Together with the fact that α-tocopherol induced N-acetylglucosaminyltransferase 3, our data suggest that α-tocopherol modifies glycoprotein metabolism partially by conferring mild ER stress. This adds another molecular mechanism of α-tocopherol toward cancer prevention. © 2006 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.abb.2006.07.002

Overexpression of LYL1 gene induces T- and B-cell leukemia in mice

Zhong, Y; Jiang, L; Toyokuni, S; Hiai, H; Yamada, Y

EXPERIMENTAL HEMATOLOGY 34 巻 ( 9 ) 頁： 47 - 47 2006年9月

Overexpression of LYL1 gene induces T- and B-cell leukemia in mice 国際誌

Zhong, Y, Jiang, L, Toyokuni, S, Hiai, H, Yamada, Y

EXPERIMENTAL HEMATOLOGY 34 巻 ( 9 ) 頁： 47 - 47 2006年9月

Historical aspects of free radical chemistry and biology: What's new in oxidative stress-induced carcinogenesis and its prevention? 査読有り国際誌 Open Access

Toyokuni Shinya

JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION 39 巻 ( 1 ) 頁： 1 - 2 2006年7月

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