研究者詳細 - 豊國　伸哉

論文 - 豊國　伸哉

分割表示 1,236 件中 701 - 720 件目／全件表示 >>

An N-glycan structure correlates with pulmonary metastatic ability of cancer cells 査読有り国際誌 Open Access

Sakuma, K; Fujimoto, I; Hitoshi, S; Tanaka, F; Ikeda, T; Tanabe, K; Toyokuni, S; Wada, H; Mio, T; Mishima, M; Ikenaka, K

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 340 巻 ( 3 ) 頁： 829 - 835 2006年2月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Biochemical and Biophysical Research Communications

N-Glycan structures on the surface of cancer cells have diverse structures and play significant roles in metastatic process. However, little is known about their roles in organ-selective metastasis. Our study revealed that an α1,6-fucosylated biantennary N-glycan structure designated A2G2F is characteristic of lungs, with far more abundant expression in normal human and murine lungs than in other organs. In this study, we further examined the role of A2G2F in pulmonary metastasis. We stained metastatic cancers by α1,6-fucose-specific Lens culinaris agglutinin lectin and revealed that pulmonary metastatic nodules more abundantly expressed α1,6-fucosylated N-glycans than hepatic metastatic nodules from common primary cancers. The most specific α1,6-fucosylated N-glycan structure in pulmonary metastatic cancer was identified to be A2G2F. Using a B16 melanoma cell metastasis model, we showed that A2G2F-rich B16 cells formed more pulmonary metastatic nodules than A2G2F-poor cells. Our results suggest that A2G2F plays a critical role in pulmonary metastasis. © 2005 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.bbrc.2005.12.072

Differences in oxidative stress dependence between gastric adenocarcinoma subtypes 査読有り

Brigitte Bancel, Jacques Esteve, Jean-Christophe Souquet, Shinya Toyokuni, Hiroshi Ohshima, Brigitte Pignatelli

WORLD JOURNAL OF GASTROENTEROLOGY 12 巻 ( 7 ) 頁： 1005 - 1012 2006年2月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：W J G PRESS

AIM: To investigate the extent of oxidative stress in pre-neoplastic and neoplastic gastric mucosa in relation to their pathological criteria and histological subtypes.
METHODS: A total of 104 gastric adenocarcinomas from 98 patients (88 infiltrative and 16 intraepithelial tumors) were assessed immunohistochemically for expression of iNOS and occurrence of nitrotyrosine (NTYR)-containing proteins and 8-hydroxy-2'-deoxyguanosine (8-OH-dG)-containing DNA, as markers of NO production and damages to protein and DNA.
RESULTS: Tumor cells staining for NOS, NTYR and 8-OH-dG were detected in 41%, 62% and 50% of infiltrative carcinoma, respectively. The three markers were shown for the first time in intraepithelial carcinoma. The expression of iNOS was significantly more frequent in tubular carcinoma (TC) compared to diffuse carcinoma (DC) (54% vs 18%; P = 0.008) or in polymorphous carcinoma (PolyC) (54% vs 21%; P = 0.04). NTYR staining was obviously more often found in TC than that in PolyC (72% vs 30%; P = 0.03). There was a tendency towards a higher rate of iNOS staining when distant metastasis (pM) was present. In infiltrative TC, the presence of oxidative stress markers was not significantly correlated with histological grade, density of inflammation, the depth of infiltration (pT), lymph nodes dissemination (pN) and pathological stages (pTNM).
CONCLUSION: The iNOS-oxidative pathway may play an important role in TC, but moderately in PolyC and DC. DNA oxidation and protein nitration occur in the three subtypes. Based on the significant differences of NTYR levels, TC and PolyC appear as two distinct subtypes. (C) 2006 The WJG Press. All rights reserved.

Web of Science

researchmap

A novel screening method for ultraviolet protection: combination of a human skin-equivalent model and 8-hydroxy-2'-deoxyguanosine. 査読有り

Toyokuni S, Yasui H, Date A, Hakozaki T, Akatsuka S, Kohda H, Yoshii T and Sakurai H.

Pathol. Int. 56 巻頁： 760-762 2006年

Anchorage-independent growth of mouse male germline stem cells in vitro. 査読有り

Shinohara M, Inoue K, Lee J, Miki H, Ogonuki N, Toyokuni S, Ogura A and Shinohara T.

Biol. Reprod. 74 巻頁： 522-529 2006年

An N-glycan structure correlates with pulmonary metastatic ability of cancer cells. 査読有り

Sakuma K, Fujimoto I, Hitoshi S, Tanaka F, Ikeda T, Tanabe K, Toyokuni S, Wada H, Mio T, Mishima M, Ikenaka K.

Biochem. Biophys. Res. Commun. 349 巻頁： 829-835 2006年

Novel aspects of oxidative stress-associated carcinogenesis. 査読有り

Toyokuni S.

Antiox. Redox Signal. 8 巻頁： 1373-1377 2006年

Differences in oxidative stress dependence between gastric adenocarcinoma subtypes. 査読有り

Bancel B, Esteve J, Souquet JC, Toyokuni S, Ohshima H, Pignatelli B.

World J. Gastroenterol. 12 巻頁： 1005-1012 2006年

What has been learned from the studies of oxidative stress-induced carcinogenesis: proposal of the concept of oxygenomics. 査読有り

Toyokuni S, Akatsuka S.

J. Clin. Biochem. Nutr. 39 巻頁： 3-10 2006年

Production of knockout mice by random or targeted mutagenesis in spermatogonial stem cells. 査読有り

Kanatsu-Shinohara M, Ikawa M, Takehashi M, Ogonuki N, Miki H, Inoue K, Kazuki Y, Lee J, Toyokuni S, Oshimura M, Ogura A, Shinohara T.

Proc. Natl. Acad. Sci. USA 103 巻頁： 8018-8023 2006年

alfa-Tocopherol induces calnexin in renal tubular cells: Another protective mechanism against free radical-induced cellular damage. 査読有り

Lee W-H, Akatsuka S, Shirase T, Dutta KK, Jiang L, Liu Y-T, Onuki J, Yamada Y, Okawa K, Wada Y, Watanabe A, Kohro T, Noguchi N and Toyokuni S.

Arch. Biochem. Biophys. 453 巻頁： 168-178 2006年

*Contrasting Genome-wide Distribution of 8-Hydroxyguanine and Acrolein-modified Adenine during Oxidative Stress-induced Renal Carcinogenesis. 査読有り

Akatsuka S, Aung TT, Dutta KK, Jiang L, Lee W-H, Liu Y-T, Onuki J, Shirase T, Yamasaki K, Ochi H, Naito Y, Yoshikawa T, Kasai H, Tominaga Y, Sakumi K, Nakabeppu Y, Kawai Y, Uchida K, Yamasaki A, Tsuruyama T, Yamada Y and Toyokuni S.

Am. J. Pathol. 169 巻頁： 1328-1342 2006年

Deletion and single nucleotide substitution at G:C are major mutations in the kidney of gpt delta transgenic mice after the Fenton reaction. 査読有り

Jiang L, Zhong Y, Akatsuka S, Liu Y-T, Dutta KK, Lee W-H, Onuki J, Masumura K, Nohmi T and Toyokuni S.

Cancer Sci. 97 巻頁： 1159-1167 2006年

Involvement of aminoacylase 1 in ferric nitrilotriacetate (Fe-NTA)-induced rat renal cell carcinoma

Onuki, J; Liu, Y; Lee, W; Jiang, L; Dutta, KK; Akatsuka, S; Toyokuni, S

FREE RADICAL RESEARCH 40 巻頁： S128 - S128 2006年

Iron-Induced Carcinogenesis 査読有り国際誌

Toyokuni, S

OXIDATIVE STRESS, DISEASE AND CANCER 頁： 789 - 805 2006年

Novel aspects of oxidative stress-associated carcinogenesis

Toyokuni Shinya

ANTIOXIDANTS & REDOX SIGNALING 8 巻 ( 7-8 ) 頁： 1373 - 1377 2006年

酸化ストレスと癌国際誌

豊國伸哉

日本トキシコロジー学会学術年会 33 巻 ( 0 ) 頁： 15 - 15 2006年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：日本毒性学会

「フリーラジカル・活性酸素による負荷から、抗酸化分子・抗酸化酵素・修復酵素などを介した消去・防御・修復能を差し引いたもの」が酸化ストレスの概念である。ここでは、発がんおよびがん領域における酸化ストレス研究の現状ならびにどのようなアプローチが可能かに関して新旧併せて紹介する。私たちは、鉄キレート剤投与によりFenton反応を尿細管にひき起こす腎傷害・発がんモデルを詳細に検討してきた。通常のパラフィン包埋標本上で酸化ストレスの可視化が可能となったのは90年代になってからであった。フリーラジカル反応で特異的に発生しかつ標本内に固定される epitopeに対する抗体を作製がなされた。脂質過酸化最終産物の一つである4-hydroxy-2-nonenalがMichael付加反応で蛋白と反応したhemiacetal構造、DNA修飾塩基である8-hydroxy-2'-deoxyguanosineやacrolein-deoxyadenosineが代表的 epitope である。これらの方法は既に定法として酸化ストレス病態の解析に広く使用されている。フリーラジカルはその反応性の高さより近傍にある分子と反応するため、反応選択性は低いと考えられてきた。私たちは、上記腎がんモデルにおいてF1動物を使用して遺伝学的解析を行い、主な標的遺伝子のひとつがp16INK4A がん抑制遺伝子であることを見いだした。しかもその変化は初期に発生する。発がんにおいては選択という要素があるが、フリーラジカルの反応は生体内において、特異的な表現型となりうることが判明してきた。最後に、がん発生の起点であるゲノム情報の変化に関して私たちがoxygenomicsと呼ぶ新しいアプローチを紹介する。 参考文献：1. Toyokuni S. Pathol Int 49: 91, 1999. 2. Tanaka T et al. Oncogene 18, 3793, 1999. 3. Hiroyasu et al. 160: 419, 2002. 4. Dutta KK et al. Lab Invest 85: 768, 2005.

DOI： 10.14869/toxp.33.0.15.0

CiNii Research

researchmap

Molecular characteristics of ferric nitrilotriacetate-induced deletions and base substitutions in the kidney of gpt delta transgenic mice

Jiang, L; Zhong, Y; Akatsuka, S; Onuki, J; Masumura, K; Nohmi, T; Toyokuni, S

FREE RADICAL RESEARCH 40 巻頁： S128 - S128 2006年

Iron-Induced Carcinogenesis

Toyokuni Shinya

OXIDATIVE STRESS, DISEASE AND CANCER 頁： 789 - 805 2006年

Deletion and single nucleotide substitution at G:C are major mutations in the kidney of gpt delta transgenic mice after the Fenton reaction. 査読有り

Jiang L, Zhong Y, Akatsuka S, Liu Y-T, Dutta KK, Lee W-H, Onuki J, Masumura K, Nohmi T, Toyokuni S

Cancer Sci. 97 巻頁： 1159-1167 2006年

Contrasting genome-wide distribution of 8-hydroxyguanine and acrolein-modified adenine during oxidative stress-induced renal carcinogenesis

Akatsuka S., Aung T. T., Dutta K. K., Jiang L., Liu Y., Lee W., Onuki J., Shirase T., Naito Y., Yoshikawa T., Uchida K., Toyokuni S.

FREE RADICAL RESEARCH 40 巻頁： S129 - S129 2006年

　前のページ - 次のページ　

<豊國 伸哉>

論文 - 豊國 伸哉

<豊國　伸哉>

論文 - 豊國　伸哉