研究者詳細 - 佐藤　ちひろ

論文 - 佐藤　ちひろ

分割表示 376 件中 161 - 180 件目／全件表示 >>

Diverse subcellular localizations of the insect CMP-sialic acid synthetases. 査読有り

Di W, Fujita A, Hamaguchi K, Delannoy P, Sato C, Kitajima K.

Glycobiology. 2016年12月

Effects of intronic single nucleotide polymorphisms (iSNPs) of a polysialyltransferase, ST8SIA2 gene found in psychiatric disorders on its gene products. 査読有り

Hane M, Kitajima K, Sato C

Biochemical and biophysical research communications 478 巻 ( 3 ) 頁： 1123 - 9 2016年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Biochemical and Biophysical Research Communications

Polysialic acid (polySia) is a linear homopolymer of sialic acid and mainly modifies neural cell adhesion molecule. PolySia plays important roles in synapse formation, learning and memory, social behavior and is associated with several diseases. Gene analyses of one of the biosynthetic enzymes for polySia, ST8SIA2, have revealed that several SNPs and genetic variations in the ST8SIA2 gene are associated with several psychiatric disorders; however, the mechanisms underlying these associations remain unknown. Here, we analyzed the effects of two iSNPs of ST8SIA2, rs2168351 and rs3784730, which are associated with bipolar disorder and autism spectrum disorder, respectively, on the expression of mRNA, ST8SIA2 and its final product, polySia in mouse neuroblastoma and human adenocarcinoma cell lines. We found that both iSNPs affected the expression of pre-mRNA and mRNA of ST8SIA2, and altered the cellular levels of ST8SIA2 and polySia. Taken together, these results indicate that impairment of the regulated expression of ST8SIA2 and the resulting downstream effects on gene products by these two iSNPs contribute to the development of these psychiatric disorders.

DOI： 10.1016/j.bbrc.2016.08.079

Web of Science

Scopus

PubMed

Effects of intronic single nucleotide polymorphisms (iSNPs) of a polysialyltransferase, ST8SIA2 gene found in psychiatric disorders on its gene products. 査読有り

Hane M, Kitajima K, Sato C.

Biochem Biophys Res Commun. 478 巻 ( 3 ) 頁： 1123-9. 2016年9月

Relationship between ST8SIA2, polysialic acid and its binding molecules, and psychiatric disorders

Sato C.

Biochimica et Biophysica Acta - General Subjects 1860 巻 ( 8 ) 頁： 1739 - 1752 2016年8月

Relationship between ST8SIA2, polysialic acid and its binding molecules, and psychiatric disorders. 招待有り査読有り

Sato C, Hane M, Kitajima K.

Biochim Biophys Acta. 1860 巻 ( 8 ) 頁： 1739-52 2016年8月

Relationship between ST8SIA2, polysialic acid and its binding molecules, and psychiatric disorders.

Sato C, Hane M, Kitajima K

Biochimica et biophysica acta 1860 巻 ( 8 ) 頁： 1739 - 52 2016年8月

Relationship between ST8SIA2, polysialic acid and its binding molecules, and psychiatric disorders. 査読有り

Sato C, Hane M, Kitajima K

Biochimica et biophysica acta 1860 巻 ( 8 ) 頁： 1739 - 1752 2016年8月

Relationship between ST8SIA2, polysialic acid and its binding molecules, and psychiatric disorders 査読有り

Chihiro Sato, Masaya Hane, Ken Kitajima

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1860 巻 ( 8 ) 頁： 1739 - 1752 2016年8月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCIENCE BV

Polysialic acid (polySia, PSA) is a unique and functionally important glycan, particularly in vertebrate brains. It is involved in higher brain functions such as learning, memory, and social behaviors. Recently, an association between several genetic variations and single nucleotide polymorphisms (SNPs) of ST8SIA2/STX, one of two polysialyltransferase genes in vertebrates, and psychiatric disorders, such as schizophrenia (SZ), bipolar disorder (BD), and autism spectrum disorder (ASD), was reported based on candidate gene approaches and genome-wide studies among normal and mental disorder patients. It is of critical importance to determine if the reported mutations and SNPs in ST8SIA2 lead to impairments of the structure and function of polySia, which is the final product of ST8SIA2. To date, however, only a few such forward-directed studies have been conducted. In addition, the molecular mechanisms underlying polySia-involved brain functions remain unknown, although polySia was shown to have an anti-adhesive effect. In this report, we review the relationships between psychiatric disorders and polySia and/or ST8SIA2, and describe a new function of polySia as a regulator of neurologically active molecules, such as brain-derived neurotrophic factor (BDNF) and dopamine, which are deeply involved in psychiatric disorders. This article is part of a Special Issue entitled "Glycans in personalised medicine" Guest Editor: Professor Gordan Lauc. (C) 2016 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.bbagen.2016.04.015

Web of Science

Relationship between ST8SIA2, polysialic acid and its binding molecules, and psychiatric disorders

Sato Chihiro, Hane Masaya, Kitajima Ken

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1860 巻 ( 8 ) 頁： 1739 - 1752 2016年8月

The AMOR Arabinogalactan Sugar Chain Induces Pollen-Tube Competency to Respond to Ovular Guidance. 査読有り国際誌 Open Access

Mizukami AG, Inatsugi R, Jiao J, Kotake T, Kuwata K, Ootani K, Okuda S, Sankaranarayanan S, Sato Y, Maruyama D, Iwai H, Garénaux E, Sato C, Kitajima K, Tsumuraya Y, Mori H, Yamaguchi J, Itami K, Sasaki N, Higashiyama T

Current biology : CB 26 巻 ( 8 ) 頁： 1091 - 7 2016年4月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Current Biology

Precise directional control of pollen-tube growth by pistil tissue is critical for successful fertilization of flowering plants [1-3]. Ovular attractant peptides, which are secreted from two synergid cells on the side of the egg cell, have been identified [4-6]. Emerging evidence suggests that the ovular directional cue is not sufficient for successful guidance but that competency control by the pistil is critical for the response of pollen tubes to the attraction signal [1, 3, 7]. However, the female molecule for this competency induction has not been reported. Here we report that ovular methyl-glucuronosyl arabinogalactan (AMOR) induces competency of the pollen tube to respond to ovular attractant LURE peptides in Torenia fournieri. We developed a method for assaying the response capability of a pollen tube by micromanipulating an ovule. Using this method, we showed that pollen tubes growing through a cut style acquired a response capability in the medium by receiving a sufficient amount of a factor derived from mature ovules of Torenia. This factor, named AMOR, was identified as an arabinogalactan polysaccharide, the terminal 4-O-methyl-glucuronosyl residue of which was necessary for its activity. Moreover, a chemically synthesized disaccharide, the β isomer of methyl-glucuronosyl galactose (4-Me-GlcA-β-(1→6)-Gal), showed AMOR activity. No specific sugar-chain structure of plant extracellular matrix has been identified as a bioactive molecule involved in intercellular communication. We suggest that the AMOR sugar chain in the ovary renders the pollen tube competent to the chemotropic response prior to final guidance by LURE peptides.

DOI： 10.1016/j.cub.2016.02.040

Web of Science

Scopus

PubMed

.The AMOR Arabinogalactan Sugar Chain Induces Pollen-Tube Competency to Respond to Ovular Guidance. 査読有り

Curr Biol. 26 巻 ( 8 ) 頁： 1091-7 2016年4月

2,11-Dibromo-13,14-dimesityl-5,8-dioxapentaphene: A Stable and Twisted Polycyclic System Containing the o-Quinodimethane Skeleton.

Sato C, Suzuki S, Kozaki M, Okada K

Organic letters 18 巻 ( 5 ) 頁： 1052 - 5 2016年3月

Flagellasialin: Highly Glycosylated Gpi-Anchored Protein Involved in Intracellular Ca<sup>2+</sup> Regulation in Sea Urchin Sperm 査読有り

Kanazawa T., Suzuki E., Miyata S., Sato C., Kitajima K.

Glycoscience: Biology and Medicine 頁： 883 - 890 2015年1月

記述言語：英語掲載種別：論文集(書籍)内論文出版者・発行元：Glycoscience: Biology and Medicine

Very little knowledge has been available on structure and function of heavily glycosylated proteins, whose glycan parts amount to more than 90 % of the whole molecules, mainly because of technical difficulties in detection and determination of structure. In 2004 flagellasialin was discovered as a major cell surface component in the lipid rafts of sea urchin sperm. The extremely high content of glycan chains completely prevented detecting this glycoprotein by conventional protein staining methods, such as Coomassie Brilliant Blue and silver staining. To make this glycoprotein visible, monoclonal antibodies 4F7 and 3G9 were developed as specific probes for detecting glycan chains of flagellasialin. The frequent occurrence of GPI-anchored proteins in lipid rafts is well recognized. Flagellasialin was actually demonstrated to contain a GPI-anchor, based on three lines of experiments. In addition to the computational predictions from the cDNA sequence and the phosphatidylinositolspecific phospholipase C treatment of the sperm membrane fraction, nitrous acid deamination of a flagellasialin-blotted membrane was newly introduced. The immunostaining of flagellasialin on the membrane disappeared after the nitrous acid treatment of the blotted membrane. This method was easy and effective for identifying flagellasialin as a GPI-anchored protein.

DOI： 10.1007/978-4-431-54841-6_167

Scopus

Polysialyltransferase assay 査読有り

Hane M., Kitajima K., Sato C.

Glycoscience: Biology and Medicine 頁： 529 - 534 2015年1月

記述言語：英語掲載種別：論文集(書籍)内論文出版者・発行元：Glycoscience: Biology and Medicine

It has been shown that polysialic acid (polySia) is an indispensable component for viability of mouse during development. Especially, it is deeply involved in normal brain function in mouse and human, affecting cell adhesion, cell migration, cell growth, neurogenesis, and synaptogenesis. PolySia is synthesized by two polysialyltransferases, ST8SIA2 and ST8SIA4. Mice deficient in both enzymes show lethality soon after birth. Mice deficient in either ST8SIA2 or ST8SIA4 show significant impairments in learning, memory, circadian rhythm, and social behavior. Interestingly, different phenotypes are often observed between ST8SIA2- and ST8SIA4-deficient mice. Therefore, characterization of these enzymes in vitro and in vivo and precise understanding of the structurefunction relationship of polySia become more and more important. In this chapter, assay procedures for in vitro activity of the polysialyltransferases are focused on to obtain clear results of their in vitro activities that are often said to be very weak compared with other sialyltransferases.

DOI： 10.1007/978-4-431-54841-6_118

Scopus

Polysialic acid 査読有り

Sato C., Kitajima K.

Glycoscience: Biology and Medicine 頁： 519 - 528 2015年1月

記述言語：英語掲載種別：論文集(書籍)内論文出版者・発行元：Glycoscience: Biology and Medicine

Polysialic acid (polySia), particularly α2,8-linked polyNeu5Ac (DP ¼ 8 ~ 400), is a unique polymer of sialic acid (Sia) and modifies neural cell adhesion molecule (NCAM) spatiotemporally in embryonic brains. PolySia is involved in cell migration, neural outgrowth, axonal guidance, synaptic plasticity, and the development of normal neural circuits and neurogenesis due to its anti- adhesive property and spatiotemporal expression. Recent improvements of polySia-detection methods have told us that polySia expression persists in certain areas of the adult brain, such as the olfactory bulb, hippocampus, subventricular zone, thalamus, prefrontal cortex, and amygdala, where neural plasticity, remodeling of neural connections, or neural generation is ongoing. Importantly, several lines of evidence have raised a new concept of polySia function that polySia works as an attractive field that associates with particular ion channels and neurologically active molecules, such as neurotrophic factor (BDNF), growth factor (FGF2), and neurotransmitter (dopamine), to regulate their involved signaling. It has been shown that polySia is involved in learning, memory, circadian rhythm, and social behaviors using polySia-impaired mice. In addition, SNPs and some deletions in genes encoding the polysialyltransferase and NCAM have been reported from patients of schizophrenia, mood disorder and autism. Such impairments of polySia structure derived from polysialyltransferase gene alterations might influence the polySia function not only as the repulsive field but also as the attractive field.

DOI： 10.1007/978-4-431-54841-6_117

Scopus

Protective effects of polysialic acid on proteolytic cleavage of FGF2 and proBDNF/BDNF. 査読有り

Hane M, Matsuoka S, Ono S, Miyata S, Kitajima K, Sato C.

Glycobiology 25 巻頁： 1112-24 2015年

Sulfatide-Hsp70 interaction promotes Hsp70 clustering and stabilizes binding to unfolded protein. 査読有り

Harada Y, Sato C, Kitajima K.

Biomolecules. 5 巻頁： 958-73 2015年

Ganglioside contained in the neuronal tissue-enriched acidic protein of 22 kDa (NAP-22) fraction prepared from the detergent-resistant membrane microdomain of rat brain inhibits the phosphatase activity of calcineurin. 査読有り

Kobayashi Y, da Silva R, Kumanogoh H, Miyata S, Sato C, Kitajima K, Nakamura S, Morita M, Hayashi F, Maekawa S.

J Neurosci Res. 93 巻頁： 1462-70 2015年

Rapid Trimming of Cell Surface Polysialic Acid (PolySia) by Exovesicular Sialidase Triggers Release of Preexisting Surface Neurotrophin. 査読有り

Sumida M, Hane M, Yabe U, Shimoda Y, Pearce OM, Kiso M, Miyagi T, Sawada M, Varki A, Kitajima K, Sato C.

J Biol Chem. 290 巻頁： 13202-14 2015年

Discovery, primary, and crystal structures and capacitation-related properties of a prostate-derived heparin-binding protein WGA16 from boar sperm. 査読有り

Garénaux E, Kanagawa M, Tsuchiyama T, Hori K, Kanazawa T, Goshima A, Chiba M, Yasue H, Ikeda A, Yamaguchi Y, Sato C, Kitajima K.

J Biol Chem. 290 巻頁： 5484-501 2015年

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