研究者詳細 - 佐藤　ちひろ

論文 - 佐藤　ちひろ

分割表示 401 件中 1 - 20 件目／全件表示 >>

Recent advances in biology of modified sialic acids (Sias), sulfated Sia and deaminoneuraminic acid (Kdn)

Kitajima, K; Nakagawa, T; Yamashita, S; Omoto, T; Wu, D; Hane, M; Sato, C

GLYCOBIOLOGY 35 巻 ( 11 ) 頁： 10 - 10 2025年11月

Association between SIGLEC16 gene polymorphisms and the risk of neurodegenerative diseases

Inokuchi, J; Tadaka, S; Inamori, KI; Nitta, T; Yamaguchi, Y; Nishihara, S; Aoki-Kinoshita, KF; Togayachi, A; Angata, K; Sato, C; Hane, M; Furukawa, JI; Yamamoto, M; Kinoshita, K

GLYCOBIOLOGY 35 巻 ( 11 ) 頁： 23 - 23 2025年11月

A highly sensitive quantitative method of polysialic acid reveals its unique changes in brain aging and neuropsychiatric disorders. Open Access

Hane M, Naramura A, Hayakawa K, Abe C, Nakagawa T, Kushima I, Furukawa S, Fukami Y, Ikegami K, Saigoh K, Kusunoki S, Katsuno M, Ozaki N, Kitajima K, Sato C

Scientific reports 15 巻 ( 1 ) 頁： 19033 2025年5月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Scientific Reports

Polysialic acid (polySia), a glycoepitope critical for neural development and plasticity, remains difficult to quantify owing to its structural complexity. Here, we present a highly sensitive sandwich enzyme-linked immunosorbent assay (ELISA) utilizing novel probes to measure polySia expression. Using this method, we quantified polySia levels in mouse brain samples across various developmental and aging stages. Notable age-related changes were observed, particularly in neuroplastic regions such as the hippocampus and olfactory bulb, where polySia levels increased at 12 months, potentially reflecting resilience mechanisms against brain aging. Elevated polySia levels in blood samples were also detected in both a schizophrenia mouse model and human patients, with a notable male preponderance. In contrast, no significant changes were observed in patients with chronic inflammatory demyelinating polyneuropathy. These findings, enabled by the novel probes, highlight a potential role for polySia in brain aging and neuropsychiatric disorders, offering new insights into developmental and disease mechanisms and supporting its utility as a diagnostic biomarker for brain impairments.

DOI： 10.1038/s41598-025-02583-x

Anemonefish use sialic acid metabolism as Trojan horse to avoid giant sea anemone stinging. Open Access

Roux N, Delannoy C, Yu SY, Miura S, Carlu L, Besseau L, Nakagawa T, Sato C, Kitajima K, Guerardel Y, Laudet V

BMC biology 23 巻 ( 1 ) 頁： 39 2025年2月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：BMC Biology

Background: Anemonefish association with sea anemones is a prime example of mutualistic symbiosis. These fish live inside the sea anemone, benefitting from the protection of its toxic nematocysts, and in return, protect the anemone from its own predators. How anemonefish manage to avoid their host toxic stings remains unclear. One hypothesis suggests that low levels of sialic acids in anemonefish mucus prevent nematocyst discharge. Results: This study verified four predictions: (i) anemonefish mucus has lower sialic acid levels than non-symbiotic damselfish; (ii) this reduction is specific to mucus; (iii) during development, sialic acid levels inversely correlate with protection; (iv) sea anemone mucus has minimal sialic acids. Conclusions: We conclude that anemonefish regulates the level of sialic acids in their mucus to avoid nematocyst discharge. We also highlight several genes implicated in sialic acid removal that could explain the protection mechanisms in place. This mechanism, potentially used by Dascyllus trimaculatus juveniles, suggests a convergent strategy for mutualistic associations with sea anemones.

DOI： 10.1186/s12915-025-02144-8

Serum glycobiomarkers for chronic inflammatory demyelinating polyneuropathy. Open Access

Furukawa S, Fukami Y, Hanamatsu H, Yokota I, Furukawa JI, Hane M, Kitajima K, Sato C, Hiraga K, Satake Y, Yagi S, Koike H, Katsuno M

European journal of neurology 32 巻 ( 1 ) 頁： e70023 2025年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：European Journal of Neurology

Background: This study conducted a comprehensive glycan analysis of serum to determine how glycan biomarkers are associated with the pathophysiology of chronic inflammatory demyelinating polyneuropathy (CIDP) and the effects of its treatment. Methods: We comparatively analyzed N- and O-glycans in the pretreatment serum of 27 treatment-naïve patients with typical CIDP and 20 age- and sex-matched healthy controls (HC) using mass spectrometry. We determined the association between clinical parameters and glycans. The serum glycan and neurofilament light-chain (NfL) levels were assessed at the baseline, and treatment response was defined according to the degree of improvement in the modified Rankin scale 12 weeks after the first dose of intravenous immunoglobulin (IVIg). Results: Compared with the HC, the CIDP group demonstrated significantly lower levels of serum total N-glycans (CIDP, median 973.3 [IQR 836.2–1131.3] pmol/μL; HC, 1125.0 [1005.0–1236.2] pmol/μL; p < 0.05), especially sialylated N-glycans (CIDP, 898.0 [752.2–1037.2] pmol/μL; HC, 1064.4 [942.7–1189.8] pmol/μL; p < 0.01). In contrast, the O-glycan levels did not differ significantly between the two groups. The treatment response was associated with low N-glycan levels, but not with the serum NfL levels. Low levels of sialylated N-glycans were associated with resistance to treatment over 12 weeks, with an area under the curve of 0.822 (p < 0.01). Conclusions: Low levels of sialylated N-glycans could potentially serve as a novel biomarker, reflecting pathophysiology and therapeutic resistance in typical CIDP.

DOI： 10.1111/ene.70023

New sialic acid-binding site of SIGLEC-7 査読有り

羽根正弥, 佐藤ちひろ

Advances in Experimental Medicine and Biology 2025年

An enhanced dual detection of DMB-labeled sialic acids using high-resolution accurate mass spectrometry and fluorescence detection. Open Access

Omoto T, Yamakawa N, Wu D, Mori H, Takeda U, Hane M, Kitajima K, Sato C

BBA advances 7 巻頁： 100152 2025年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Bba Advances

Sialic acids are important glycan components not only in the conformation of the structure but also in functions of many biological activities. Detection methods for sialic acid have been developed, and fluorescent labeling of sialic acids with 1,2-diamino-4,5-methylenedioxybenzene (DMB) is the best method for its analysis. However, identifying and quantifying sialic acids using mass spectrometry (MS) remains difficult because of the diversity of sialic acids, including O-acetylation. In this study, we initially focused on enhancing the liquid chromatography (LC) separation of DMB-labeled sialic acids in bovine submaxillary mucin (BSM). We successfully achieved highly resolutive separation of 14 types of sialic acids, including multiply O-acetylated species, using a CAPCELL CORE C18 column. These structures were subsequently confirmed through collision-induced dissociation (CID) fragmentation. We then assessed MS and fluorescence detection (FLD) sensitivity using Neu5Ac, Neu5Gc, and Kdn standards, determining detection limits of 32 fmol for MS and 320 amol for FLD, with 320 fmol needed for CID-based structural analysis. These findings highlight the complementary nature of fluorescence and mass spectrometry techniques for DMB-labeled sialic acids identification and quantification. We also examined both CID and electron-activated dissociation (EAD) on the ZenoTOF 7600. CID fragment analysis revealed C5-specific diagnostic fragment ions for Neu5Ac, Neu5Gc, and Kdn. EAD spectra predominantly induced fragmentation of the fluorescent DMB core, regardless of applied variable kinetic energies. This study also led to the discovery of a new structure, Neu5Gc7,8,9Ac<inf>3</inf>, alongside the previously reported Neu5,7,8,9Ac<inf>4</inf> in BSM.

DOI： 10.1016/j.bbadva.2025.100152

Development of a permethylation-based detection method for oligo/polysialic acid structures via tandem MALDI-TOF mass spectrometry. Open Access

Suzuki K, Wu D, Kitajima K, Yamakawa N, Omoto T, Hane M, Sato C

BBA advances 7 巻頁： 100155 2025年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Bba Advances

Oligo/polysialic acid (oligo/polySia) refers to a class of linear polymers composed of Sias, found extensively at the non-reducing ends of glycans across various organisms, from bacteria to vertebrates. Different types of oligo/polySia have been characterized, varying in Sia composition, α-ketosidic linkages to penultimate Sia residues, and degree of polymerization. Detection of oligo/polySia-capped glycans is typically achieved through western blotting or ELISA using specific antibodies or endo-N-acylneuraminidase. Additionally, a sensitive chemical approach involving the direct labeling of oligo/polySia with 1,2-diamino-4,5-methylenedioxybenzene, followed by separation and quantification via anion-exchange high-performance liquid chromatography, has been developed. However, detection through mass spectrometry (MS) has remained challenging due to the multiple negative charges and structural instability of these glycans. In this study, we applied a permethylation technique to convert carboxyl group of Sias into methyl esters, thereby neutralizing their negative charges, which allowed for improved MS analysis of sialylated glycans. We optimized both the permethylation and glycan purification processes. Consequently, we achieved the first successful detection of polySia structures using MALDI-TOF MS. There are more in-source decay ions in the MS profiling of permethylated α2,9-linked polySias compared to α2,8-linked polySias. Furthermore, we present the first examples of oligo/polySia sequencing by collision-induced dissociation on a TOF/TOF instrument, enabling us to differentiate between α2,8- and α2,9-linked polySias. We extended this approach to analyze oligo/polySia structures with O-linked glycans in polysialoglycoprotein from salmonid eggs. For the first time, the analysis of intact oligo/polySia structures with O-linked glycans were successfully detected using MALDI-TOF/TOF MS/MS with this optimized permethylation method.

DOI： 10.1016/j.bbadva.2025.100155

The Human Glycome Atlas project for cataloging all glycan-related omics data in human. Open Access

Aoki-Kinoshita KF, Akune-Taylor Y, Ando H, Angata K, Fujita M, Furukawa JI, Kaji H, Kato K, Kitajima K, Kizuka Y, Matsui Y, Nakajima K, Nishihara S, Okajima T, Sakamoto K, Sato C, Thaysen-Andersen M, Togayachi A, Yagi H, Zappa A, Kadomatsu K

Glycobiology 34 巻 ( 11 ) 2024年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Glycobiology

The Human Glycome Atlas (HGA) Project was launched in April 2023, spearheaded by three Japanese institutes: the Tokai National Higher Education and Research System, the National Institutes of Natural Sciences, and Soka University. This was the first time that a field in the life sciences was adopted by the Ministry of Education, Culture, Sports, Science and Technology (MEXT) for a Large-scale Academic Frontiers Promotion Project. This project aims to construct a knowledgebase of human glycans and glycoproteins as a standard for the human glycome. A high-throughput pipeline for comprehensively analyzing 20,000 blood samples in its first five years is planned, at which time an access-controlled version of a human glycomics knowledgebase, called TOHSA, will be released. By the end of the final tenth year, TOHSA will provide a central resource linking human glycan data with other omics data including disease-related information.

DOI： 10.1093/glycob/cwae052

SERUM GLYCOBIOMARKERS DEFINING THERAPEUTIC RESPONSE TO INTRAVENOUS IMMUNOGLOBULIN IN CHRONIC INFLAMMATORY DEMYELINATING POLYNEUROPATHY

Furukawa, S; Fukami, Y; Yokota, I; Hanamatsu, H; Furukawa, JI; Hane, M; Kitajima, K; Sato, C; Hiraga, K; Satake, Y; Yagi, S; Koike, H; Katsuno, M

JOURNAL OF THE PERIPHERAL NERVOUS SYSTEM 29 巻頁： S92 - S92 2024年8月

Neuraminidase inhibition promotes the collective migration of neurons and recovery of brain function. Open Access

Matsumoto M, Matsushita K, Hane M, Wen C, Kurematsu C, Ota H, Bang Nguyen H, Quynh Thai T, Herranz-Pérez V, Sawada M, Fujimoto K, García-Verdugo JM, Kimura KD, Seki T, Sato C, Ohno N, Sawamoto K

EMBO molecular medicine 16 巻 ( 6 ) 頁： 1228 - 1253 2024年6月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：EMBO Molecular Medicine

In the injured brain, new neurons produced from endogenous neural stem cells form chains and migrate to injured areas and contribute to the regeneration of lost neurons. However, this endogenous regenerative capacity of the brain has not yet been leveraged for the treatment of brain injury. Here, we show that in healthy brain chains of migrating new neurons maintain unexpectedly large non-adherent areas between neighboring cells, allowing for efficient migration. In instances of brain injury, neuraminidase reduces polysialic acid levels, which negatively regulates adhesion, leading to increased cell–cell adhesion and reduced migration efficiency. The administration of zanamivir, a neuraminidase inhibitor used for influenza treatment, promotes neuronal migration toward damaged regions, fosters neuronal regeneration, and facilitates functional recovery. Together, these findings shed light on a new mechanism governing efficient neuronal migration in the adult brain under physiological conditions, pinpoint the disruption of this mechanism during brain injury, and propose a promising therapeutic avenue for brain injury through drug repositioning.

DOI： 10.1038/s44321-024-00073-7

Assembly of neuron- and radial glial-cell-derived extracellular matrix molecules promotes radial migration of developing cortical neurons. Open Access

Mubuchi A, Takechi M, Nishio S, Matsuda T, Itoh Y, Sato C, Kitajima K, Kitagawa H, Miyata S

eLife 12 巻 2024年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：eLife Sciences Publications, Ltd

<jats:p>Radial neuronal migration is a key neurodevelopmental event for proper cortical laminar organization. The multipolar-to-bipolar transition, a critical step in establishing neuronal polarity during radial migration, occurs in the subplate/intermediate zone (SP/IZ), a distinct region of the embryonic cerebral cortex. It has been known that the extracellular matrix (ECM) molecules are enriched in the SP/IZ. However, the molecular constitution and functions of the ECM formed in this region remain poorly understood. Here, we identified neurocan (NCAN) as a major chondroitin sulfate proteoglycan in the mouse SP/IZ. NCAN binds to both radial glial-cell-derived tenascin-C (TNC) and hyaluronan (HA), a large linear polysaccharide, forming a ternary complex of NCAN, TNC, and HA in the SP/IZ. Developing cortical neurons make contact with the ternary complex during migration. The enzymatic or genetic disruption of the ternary complex impairs radial migration by suppressing the multipolar-to-bipolar transition. Furthermore, both TNC and NCAN promoted the morphological maturation of cortical neurons in vitro. The present results provide evidence for the cooperative role of neuron- and radial glial-cell-derived ECM molecules in cortical development.</jats:p>

DOI： 10.7554/eLife.92342

Rat hepatocytes secrete free oligosaccharides. 査読有り国際共著 Open Access

Huang C, Seino J, Honda A, Fujihira H, Wu D, Okahara K, Kitazume S, Nakaya S, Kitajima K, Sato C, Suzuki T

The Journal of biological chemistry 300 巻 ( 3 ) 頁： 105712 2024年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of Biological Chemistry

We recently established a method for the isolation of serum-free oligosaccharides, and characterized various features of their structures. However, the precise mechanism for how these glycans are formed still remains unclarified. To further investigate the mechanism responsible for these serum glycans, here, we utilized rat primary hepatocytes to examine whether they are able to secrete free glycans. Our findings indicated that a diverse array of free oligosaccharides such as sialyl/neutral free N-glycans (FNGs), as well as sialyl lactose/LacNAc-type glycans, were secreted into the culture medium by primary hepatocytes. The structural features of these free glycans in the medium were similar to those isolated from the sera of the same rat. Further evidence suggested that an oligosaccharyltransferase is involved in the release of the serum-free N-glycans. Our results indicate that the liver is indeed secreting various types of free glycans directly into the serum.

DOI： 10.1016/j.jbc.2024.105712

Is the linkage mode of sialic acid residues significant in normal heart development in medaka?

Kitajima, K; Omoto, T; Wu, D; Maruyama, E; Tajima, K; Hane, M; Sato, C

GLYCOBIOLOGY 33 巻 ( 11 ) 頁： 995 - 995 2023年12月

Developmental and organ-specific expression profiles of sulfated sialic acids in medaka fish as revealed by the chemical method

Yamashita, S; Wu, D; Hane, M; Komura, N; Ando, H; Sato, C; Kitajima, K

GLYCOBIOLOGY 33 巻 ( 11 ) 頁： 994 - 994 2023年12月

Forced expression of α2,3-sialyltransferase IV rescues impaired heart development in α2,6-sialyltransferase I-deficient medaka. 査読有り国際共著 Open Access

Omoto T, Wu D, Maruyama E, Tajima K, Hane M, Sato C, Kitajima K

Biochemical and biophysical research communications 649 巻頁： 62 - 70 2023年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Biochemical and Biophysical Research Communications

Sialic acids (Sias) are often linked to galactose (Gal) residues by α2,6- and α2,3-linkages in glycans of glycoproteins. Sias are indispensable for vertebrate development, because organisms deficient in some enzymes in the Sia synthetic pathway are lethal during the development. However, it remains unknown if the difference of Siaα2,6Gal or α2,3Gal linkage has a critical meaning. To find a clue to understand significance of the linkage difference at the organism level, medaka was used as a vertebrate model. In embryos, Siaα2,6Gal epitopes recognized by Sambucus nigra lectin (SNA) and Siaα2,3Gal epitopes recognized by Maackia amurensis lectin (MAA) were enriched in the blastodisc and the yolk sphere, respectively. When these lectins were injected in the perivitelline space, SNA, but not MAA, impaired embryo body formation at 1 day post-fertilization (dpf). Most Siaα2,6Gal epitopes occurred on N-glycans owing to their sensitivity to peptide:N-glycanase. Of knockout-medaka (KO) for either of two β-galactoside:α2,6-sialyltransferase genes, ST6Gal I and ST6Gal II, only ST6Gal I–KO showed severe cardiac abnormalities at 7–16 dpf, leading to lethality at 14–18 dpf. Interestingly, however, these cardiac abnormalities of ST6Gal I–KO were rescued not only by forced expression of ST6Gal I, but also by that of ST6Gal II and the β-galactoside:α2,3-sialyltransferase IV gene (ST3Gal IV). Taken together, the Siaα2,6Gal linkage synthesized by ST6Gal I are critical in heart development; however, it can be replaced by the linkages synthesized by ST6Gal II and ST3Gal IV. These data suggest that sialylation itself is more important than its particular linkage for the heart development.

DOI： 10.1016/j.bbrc.2023.01.010

Critical Role of the Cortical Alveolus Protease Alveolin in Chorion Hardening In Vivo at Medaka Fertilization. 国際共著 Open Access

Fu B, Wu D, Yasumasu S, Hane M, Sato C, Kitajima K

Biomolecules 13 巻 ( 1 ) 頁： 146 2023年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Biomolecules

Alveolin is a cortical alveolus proteinase that is secreted in the perivitelline space (PVS) at fertilization to act on the chorion. Purified alveolin is known to induce chorion hardening in vitro by processing zona pellucida B (ZPB), a major chorion component. However, in vivo function of alveolin remains unclear; thus, in this study, the effects of alveolin efficiency (Alv−/−) at the organism level were investigated using the medaka, Oryzias latipes. The Alv−/− fertilized eggs were mechanically fragile; however, they developed normally and left offspring as long as they were carefully handled before hatching. A mechanical press test showed that the Alv−/− fertilized eggs were six times more fragile than the wild-type eggs. They were 35% larger owing to the enlarged PVS, 34% thinner, and permeable to even 10 kDa FITC-dextran. These results are consistent with the transmission electron microscopy observation that the periphery of the inner layers was highly porous in the Alv−/− chorion. In chorion hardening, the alveolin-mediated processing of ZPB and the transglutaminase (TGase)-mediated crosslinking of chorion components are the key steps. This study was the first to show that alveolin also processed TGase concomitantly with ZPB, which greatly facilitated the crosslinking. Thus, alveolin was concluded to be the primary trigger for chorion hardening in vivo. Furthermore, fertilization in a balanced salt solution could partially improve the impaired chorion hardening of the Alv−/− eggs fertilized in water, probably through an alveolin-independent mechanism.

DOI： 10.3390/biom13010146

Identification and characterization of a deaminoneuraminic acid (Kdn)-specific aldolase from Sphingobacterium species.

Nakagawa T, Iwaki Y, Wu D, Hane M, Sato C, Kitajima K

Glycobiology 33 巻 ( 1 ) 頁： 47 - 56 2023年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Glycobiology

Sialic acid (Sia) is a group of acidic sugars with a 9-carbon backbone, and classified into 3 species based on the substituent group at C5 position: N-acetylneuraminic acid (Neu5Ac), N-glycolylneuraminic acid (Neu5Gc), and deaminoneuraminic acid (Kdn). In Escherichia coli, the sialate aldolase or N-acetylneuraminate aldolase (NanA) is known to catabolize these Sia species into pyruvate and the corresponding 6-carbon mannose derivatives. However, in bacteria, very little is known about the catabolism of Kdn, compared with Neu5Ac. In this study, we found a novel Kdn-specific aldolase (Kdn-aldolase), which can exclusively degrade Kdn, but not Neu5Ac or Neu5Gc, from Sphingobacterium sp., which was previously isolated from a Kdn-assimilating bacterium. Kdn-aldolase had the optimal pH and temperature at 7.0–8.0 and 50 ◦C, respectively. It also had the synthetic activity of Kdn from pyruvate and mannose. Site-specific mutagenesis revealed that N50 residue was important for the Kdn-specific reaction. Existence of the Kdn-aldolase suggests that Kdn-specific metabolism may play a specialized role in some bacteria.

DOI： 10.1093/glycob/cwac053

A novel C-domain-dependent inhibition of the rainbow trout CMP-sialic acid synthetase activity by CMP-deaminoneuraminic acid. Open Access

Wu D, Gilormini PA, Toda S, Biot C, Lion C, Guérardel Y, Sato C, Kitajima K

Biochemical and biophysical research communications 617 巻 ( Pt 1 ) 頁： 16 - 21 2022年8月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Biochemical and Biophysical Research Communications

The CMP-sialic acid synthetase (CSS) activates free sialic acid (Sia) to CMP-Sia using CTP, and is prerequisite for the sialylation of cell surface glycoconjugates. The vertebrate CSS consists of two domains, a catalytic N-domain and a non-catalytic C-domain. Although the C-domain is not required for the CSS enzyme to synthesize CMP-Sia, its involvement in the catalytic activity remains unknown. First, the real-time monitoring of CSS-catalyzed reaction was performed by 31P NMR using the rainbow trout CSS (rtCSS). While a rtCSS lacking the C-domain (rtCSS-N) similarly activated both deaminoneuraminic acid (Kdn) and N-acetylneuraminic acid (Neu5Ac), the full-length rtCSS (rtCSS-FL) did not activate Kdn as efficiently as Neu5Ac. These results suggest that the C-domain of rtCSS affects the enzymatic activity, when Kdn was used as a substrate. Second, the enzymatic activity of rtCSS-FL and rtCSS-N was measured under various concentrations of CMP-Kdn. Inhibition by CMP-Kdn was observed only for rtCSS-FL, but not for rtCSS-N, suggesting that the inhibition was C-domain-dependent. Third, the inhibitory effect of CMP-Kdn was also investigated using the mouse CSS (mCSS). However, no inhibition was observed with mCSS even at high concentrations of CMP-Kdn. Taken together, the data demonstrated that the C-domain is involved in the CMP-Kdn-dependent inhibition of rtCSS, which is a novel regulation of the Sia metabolism in rainbow trout.

DOI： 10.1016/j.bbrc.2022.05.031

Sulfation of sialic acid is ubiquitous and essential for vertebrate development. Open Access

Ertunc N, Phitak T, Wu D, Fujita H, Hane M, Sato C, Kitajima K

Scientific reports 12 巻 ( 1 ) 頁： 12496 2022年7月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Scientific Reports

Glycosylation of proteins and lipids occurs in vertebrates, usually terminating with sialylation, which regulates the physicochemical and biological properties of these glycoconjugates. Although less commonly known, sialic acid residues also undergo various modifications, such as acetylation, methylation, and sulfation. However, except for acetylation, the enzymes or functions of the other modification processes are unknown. To the best of our knowledge, this study is the first to demonstrate the ubiquitous occurrence of sulfated sialic acids and two genes encoding the sialate: O-sulfotransferases 1 and 2 in vertebrates. These two enzymes showed about 50% amino acid sequence identity, and appeared to be complementary to each other in acceptor substrate preferences. Gene targeting experiments showed that the deficiency of these genes was lethal for medaka fish during young fry development and accompanied by different phenotypes. Thus, the sulfation of sialic acids is essential for the vertebrate development.

DOI： 10.1038/s41598-022-15143-4

　前のページ - 次のページ　

<佐藤 ちひろ>

論文 - 佐藤 ちひろ

<佐藤　ちひろ>

論文 - 佐藤　ちひろ