研究者詳細 - 佐藤　ちひろ

論文 - 佐藤　ちひろ

分割表示 401 件中 261 - 280 件目／全件表示 >>

Mechanism regulating Ca2+-dependent mechanosensory behaviour in sea urchin spermatozoa. 査読有り Open Access

Kambara Y, Shiba K, Yoshida M, Sato C, Kitajima K, Shingyoji C

Cell structure and function 36 巻 ( 1 ) 頁： 69 - 82 2011年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Cell Structure and Function

Flagellar movement of the sea urchin sperm is regulated by intracellular Ca2+. Flagellasialin, a polysialic acid-containing glycoprotein, as well as other membrane proteins seems responsible for the Ca2+ control. To elucidate the mechanism of Ca2+ dynamics underlying flagellar movement, we analysed the sperm's mechanosensory behavioural responses by using microtechniques. In sea water containing 10 mM Ca2+, the sperm swim in circular paths. When a mechanical stimulus was applied to the sperm head with a glass microstylus, the sperm showed a series of flagellar responses, consisting of a stoppage of beating (quiescence) and a recovery of swimming in a straight path, followed by swimming in a circular path again; as the result the sperm avoided the obstacle. Ca2+-imaging with Fluo-4 showed that the intracellular Ca2+ was high in the quiescence and gradually decreased after that. The effects of blockers and antibodies against candidate components revealed that the Ca2+ influx was induced by Ca2+ channels and the Ca2+ efflux was induced by a flagellasialin-related Ca2+- efflux system, plasma membrane Ca2+-ATPases and the K+-dependent Na+/Ca2+ exchanger. The results show that the Ca2+-dependent mechanosensory behaviour of the sea urchin sperm is regulated by organized functioning of the membrane environment including the plasma membrane proteins and flagellasialin. © 2011 by Japan Society for Cell Biology.

DOI： 10.1247/csf.10020

Mechanism Regulating Ca2+-dependent Mechanosensory Behaviour in Sea Urchin Spermatozoa Open Access

Kambara Yuka, Shiba Kogiku, Yoshida Manabu, Sato Chihiro, Kitajima Ken, Shingyoji Chikako

CELL STRUCTURE AND FUNCTION 36 巻 ( 1 ) 頁： 69-82 2011年

Structural and functional impairments of polysialic acid by a mutated polysialyltransferase found in schizophrenia 査読有り Open Access

Isomura, R, Kitajima, K, Sato, C

J. Biol. Chem. 286 巻頁： 21535-21545 2011年

New functions of polysialic acid and its relationship to schizophrenia 査読有り Open Access

Sato C, Kitajima K

TIGG 23 巻頁： 221-238 2011年

Developmental stage-dependent expression of an alpha2,8-trisialic acid unit on glycoproteins in mouse brain. 査読有り Open Access

Inoko E, Nishiura Y, Tanaka H, Takahashi T, Furukawa K, Kitajima K, Sato C

Glycobiology 20 巻 ( 7 ) 頁： 916 - 28 2010年7月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Glycobiology

The monoclonal antibody mAb.A2B5 is a marker for the detection of oligodendrocyte progenitor cells that differentiate into type-2 astrocytes and oligodendrocytes. It is also a useful antibody for separating these cells from other lineage populations. The epitope of this antibody is considered to be the gangliosides GT3 and GQ1c. In this study, we sought to define more precisely the structure of the epitope. Accordingly, we chemically synthesized defined oligosialic acid structures linked to phosphatidylethanolamine and bovine serum albumin and used these to determine the antigenic specificity. mAb.A2B5 recognized the Neu5Acα2→8-Neu5Acα2→8Neu5Acα → structure on both glycolipids and glycoproteins. We then examined whether the mAb. A2B5 epitope exists on glycoproteins in developing mouse brains. Western blot analyses revealed the expression of four glycoproteins reactive with the mAb.A2B5, and their expression was dependent on the stage of neural development. All the immunoreactivity in these glycoproteins with mAb.A2B5 disappeared after sialidase treatment and were resistant to chloroform/methanol extraction. These epitopes were also detected in brain homogenates from both GD3 synthetase-null and GD3/GD2 synthetase double null mice. These findings show that the α 2,8- trisialic acid (triSia) unit recognized by mAb.A2B5 resides not only on gangliosides but also on glycoproteins in developing mouse brain. We postulate that the triSia structure on glycoproteins may be involved in oligodendrocyte differentiation, similar to the case with the α 2,8-triSia structure on gangliosides. Real time polymerase chain reaction analysis of the developmental expression of all known ST8Sia genes, which are responsible for the biosynthesis of α 2,8- linked Sia residues, showed that ST8Sia III gene expression correlated with expression of the triSia epitope. We suggest that ST8Sia III is the principal sialyltransferase responsible for synthesis of the α 2,8-triSia units on glycoproteins. © The Author 2010. Published by Oxford University Press. All rights reserved.

DOI： 10.1093/glycob/cwq049

Web of Science

Scopus

PubMed

糖鎖と統合失調症招待有り

佐藤ちひろ

生体の科学 61 巻頁： 160-166 2010年

IgG2 dominancy and carbohydrate recognition specificity of C3H/He mouse antibodies directed to cross-reactive carbohydrate determinants (CCDs) bearing beta-(1,2)-xylose and alpha-(1,3)-fucose. 査読有り

Hino S, Umeda F, Inumaru S, Aoki N, Sato, C., Okajima T, Nadano D, Matsuda T.

Immunol Lett 133 巻頁： 28-34 2010年

Survival signals of hepatic stellate cells in liver regeneration are regulated by glycosylation changes in rat vitronectin, especially decreased sialylation. 査読有り Open Access

Sano K, Miyamoto Y, Kawasaki N, Hashii N, Itoh S, Murase M, Date K, Yokoyama M, Sato, C., Kitajima K, Ogawa H.

J Biol Chem 285 巻頁： 17301-17309 2010年

Extensive enrichment of N-glycolylneuraminic acid in extracellular sialoglycoproteins abundantly synthesized and secreted by human cancer cells. 査読有り

Inoue, S., Sato, C. and Kitajima, K

Glycobiology 20 巻頁： 752-762 2010年

Developmental stage-dependent expression of an α2,8-trisialic acid unit on glycoproteins in mouse brain. 査読有り Open Access

Inoko, E., Nishiura, Y., Tanaka, H., Takahashi, T., Furukawa, K., Kitajima, K., and Sato, C.

Glycobiology 20 巻頁： 916-928 2010年

Survival signals of hepatic stellate cells in liver regeneration are regulated by glycosylation changes in rat vitronectin, especially decreased sialylation. 査読有り Open Access

Sano K, Miyamoto Y, Kawasaki N, Hashii N, Itoh S, Murase M, Date K, Yokoyama M, Sato, C, Kitajima K, Ogawa H

J Biol Chem 285 巻頁： 17301-17309 2010年

Extensive enrichment of N-glycolylneuraminic acid in extracellular sialoglycoproteins abundantly synthesized and secreted by human cancer cells. 査読有り

Inoue, S, Sato, C, Kitajima, K

Glycobiology 20 巻頁： 752-762 2010年

糖鎖と統合失調症招待有り

佐藤ちひろ

生体の科学 61 巻頁： 160-166 2010年

Hino S, Umeda F, Inumaru S, Aoki N, Sato, C, Okajima T, Nadano D, Matsuda T

Immunol Lett 133 巻頁： 28-34 2010年

Periodate-resistant carbohydrate epitopes recognized by IgG and IgE antibodies from some of the immunized mice and patients with allergy. 査読有り

Hino S, Matsubara T, Urisu A, Aoki N, Sato C, Okajima T, Nadano D, Matsuda T

Biochemical and biophysical research communications 380 巻 ( 3 ) 頁： 632 - 7 2009年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Biochemical and Biophysical Research Communications

The β(1,2)-xylose- and/or α(1,3)-fucose-containing cross-reactive carbohydrate determinants (CCDs) are present in various plant and insect N-glycans, and have been attracted as potential antigens in IgE-mediated allergies and immunologically undesired post-translational products on some recombinant therapeutic proteins. By using ELISA and immunoblotting, CCDs-specific IgG and IgE antibodies from some, but not all, of mice and humans were found to fully retain their binding activity after a typical periodate-treatment to CCDs, which did cause the CCDs' antigenic activity to those from the other mice and rabbits to disappear almost completely. Furthermore, the mouse IgE recognizing the periodate-resistant CCDs induced the CCDs/IgE-dependent degranulation of rat basophilic RBL-2H3 cells. These findings indicate that in some cases CCDs include those dependent of the core trisaccharide more strongly than the terminal xylose and fucose, which might have been screened out in the CCDs analyses based on the loss of antibody-binding by the periodate-treatment. © 2009 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.bbrc.2009.01.138

Web of Science

Scopus

PubMed

Membrane microdomains from early gastrula embryos of medaka, Oryzias latipes are a plat form of E-cadherin and carbohydrate-mediated cell-cell interactions during epiboly 査読有り

Adachi T., Sato, C., Kishi, Y., Totani, K., Murata, T., Usui, T., and Kitajima, K

Glycoconjugate J. 26 巻頁： 285-299 2009年

Complex formation of a brain-derived neurotrophic factor and glycosaminoglycans. 査読有り Open Access

Kanato, Y., Kitajima, K. and Sato, C.

Biosci. Biotech. Biosci. 12 巻頁： 1044-1053 2009年

Developmental regulation of oligosialylation in zebrafish. 査読有り

Chang LY, Harduin-Lepers A, Kitajima K, Sato C, Huang CJ, Khoo KH, Guérardel Y.

Glycoconjugate J. 26 巻頁： 247-61 2009年

Complex formation of a brain-derived neurotrophic factor and glycosaminoglycans. 査読有り Open Access

Kanato, Y, Kitajima, K, Sato, C

Biosci. Biotech. Biosci. 12 巻頁： 1044-1053 2009年

Developmental regulation of oligosialylation in zebrafish. 査読有り

Chang LY, Harduin-Lepers A, Kitajima K, Sato C, Huang CJ, Khoo KH, Guérardel Y

Glycoconjugate J. 26 巻頁： 247-61 2009年

　前のページ - 次のページ　

<佐藤 ちひろ>

論文 - 佐藤 ちひろ

<佐藤　ちひろ>

論文 - 佐藤　ちひろ