研究者詳細 - 佐藤　ちひろ

論文 - 佐藤　ちひろ

分割表示 401 件中 301 - 320 件目／全件表示 >>

Complex formation of 70-kDa heat shock protein with acidic glycolipids and phospholipids. 査読有り

Harada Y, Sato C, Kitajima K

Biochem Biophys Res Commun. 353 巻頁： 655-660 2007年

シアル酸の構造多様性とその生物学的意義. 招待有り

北島健, 郷慎司, 藤田明子, 佐藤ちひろ

細胞工学 26 巻頁： 629-634 2007年

Glycobiology of polysialic acids on sea urchin gametes. 招待有り査読有り Open Access

Miyata, S, Sato, C, Kitajima, K

Trends Glycosci. Glycotech. 19 巻頁： 83-95 2007年

Membrane microdomain formation is crucial in epiboly during gastrulation of medaka. 査読有り

Adachi, T, Sato, C, Kitajima, K

Biochem. Biophys. Res. Commun. 355 巻頁： 174-180 2007年

Identification of the nuclear export signals that regulate the intracellular localization of the mouse CMP-sialic acid synthetase. 査読有り

Fujita, A, Sato C, Kitajima, K

Biochem. Biophys. Res. Commun. 355 巻頁： 174-180 2007年

Hypoxia-enhanced expression of free deaminoneuraminic acid in human cancer cells. 査読有り

Go, S, Sato, C, Yin, J, Kannagi, R, Kitajima, K

Biochem. Biophys. Res. Commun. 357 巻頁： 537-542. 2007年

AMPAR removal underlies Abeta-induced synaptic depression and dendritic spine loss. Open Access

Hsieh H, Boehm J, Sato C, Iwatsubo T, Tomita T, Sisodia S, Malinow R

Neuron 52 巻 ( 5 ) 頁： 831-43 2006年12月

Flagellasialin: a novel sulfated alpha2,9-linked polysialic acid glycoprotein of sea urchin sperm flagella. 査読有り Open Access

Miyata S, Sato C, Kumita H, Toriyama M, Vacquier VD, Kitajima K

Glycobiology 16 巻 ( 12 ) 頁： 1229 - 41 2006年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Glycobiology

A novel α2,9-linked polysialic acid (polySia)-containing glycoprotein of sea urchin sperm flagella was identified and named "flagellasialin." Flagellasialin from Hemicentrotus pulcherrimus shows a diverse relative molecular mass on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of 40-80 kDa. Flagellasialin is a 96-amino acid, threonine-rich, heavily O-glycosylated (80-90% by weight) glycoprotein with a single transmembrane segment at its C-terminus and no apparent cytosolic domain. Of 12 extracellular Thr residues, eight are O-glycosylated and three are nonglycosylated. Flagellasialin is highly expressed in the testis but cannot be detected in the ovary. The amino acid sequences of flagellasialin from three sea urchin species (H. pulcherrimus, Strongylocentrotus purpuratus, and Strongylocentrotus franciscanus) are identical, but some species differences exist in the three core glycan structures to which the sulfated α2,9-linked polyNeu5Ac chain is linked. Finally, the treatment of sperm with a specific antibody against the α2,9-linked polyNeu5Ac structure results in the elevation of intracellular Ca<sup>2+</sup> and inhibition of sperm motility and fertilization, implicating flagellasialin as a regulator of these critical processes. © 2006 Oxford University Press.

DOI： 10.1093/glycob/cwl036

Web of Science

Scopus

PubMed

Identification of disialic acid-containing glycoproteins in mouse serum: a novel modification of immunoglobulin light chains, vitronectin, and plasminogen. 査読有り Open Access

Yasukawa Z, Sato C, Sano K, Ogawa H, Kitajima K

Glycobiology 16 巻 ( 7 ) 頁： 651 - 65 2006年7月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Glycobiology

Serum glycoproteins are involved in various biologic activities, such as the removal of exogenous antigens, fibrinolysis, and metal transport. Some of them are also useful markers of inflammation and disease. Although the amount of sialic acid increases following inflammation, little attention has been paid to the presence of linkage-specific epitopes in serum, especially the α2,8-linkage. In a previous study, we demonstrated that four components in mouse serum contain α2,8-linked disialic acid (diSia), based on immunoreactivity with monoclonal antibody 2-4B, which is specific to N-glycolylneuraminic acid (Neu5Gc)α2→(8Neu5Gcα2→)<inf>n-1</inf>, n ≥ 2 [Yasukawa et al., (2005) Glycobiology, 15, 827-837]. In this study, we purified three components, 30-, 70-, and 120-kDa gp, and identified them as an immunoglobulin (Ig) light chain, vitronectin, and plasminogen, respectively, using matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy analyses. Modifications of these proteins with α2,8-linked diSia were chemically confirmed by fluorometric C<inf>7</inf>/C<inf>9</inf> analyses and mild acid hydrolysates-fluorometric anion-exchange chromatography analyses. We also demonstrated that the IgG, IgM, and IgE light chains are commonly modified with α2,8-linked diSia. In addition, both mouse and rat vitronectin contained diSia, and the amount of disialylation in vitronectin dramatically decreased after hepatectomy. These results indicate that a novel diSia modification of serum glycoproteins is biologically important for immunologic events and fibrinolysis. © 2006 Oxford University Press.

DOI： 10.1093/glycob/cwj112

Web of Science

Scopus

PubMed

Hypoxic culture induces expression of Sialin, a sialic acid transporter, and cancer-associated gangliosides containing non-human sialic acid on human cancer cells. , 査読有り Open Access

Yin, J., Hashimoto, A., Izawa, M., Miyazaki, K., Chen G-Y., Takematsu, H., Kozutsumi, Y., Suzuki, A., Furuhata, K., Cheng, F.-L. Lin, C.-H., Sato, C., Kitajima, K., and Kannagi, R.

Cancer Res. 66 巻頁： 2937-2945 2006年

Flagellasialin: a novel sulfated alpha2,9-linked polysialic acid glycoprotein of sea urchin sperm flagella. 査読有り Open Access

Miyata S, Sato C, Kumita H, Toriyama M, Vacquier VD, Kitajima K.

Glycobiology 16 巻頁： 1229-12241 2006年

記述言語：英語掲載種別：研究論文（学術雑誌）

A novel α2,9-linked polysialic acid-containing glycoprotein of sea urchin sperm flagella was previously identified and named “flagellasialin". Flagellasialin from Hemicentrotus pulcherrimus shows a diverse relative molecular mass on SDS-PAGE of 40-80 kDa. Its glycan structures were determined as HSO3→8Neu5Acα2→9(Neu5Acα2→9)n-2Neu5Acα2→6GalNAcα1→Thr (n, averaging 15) [Miyata et al. (2004) Glycobiology, 14, 827-840]. In this study, we purified flagellasialin, and cloned its cDNA. Flagellasialin is a 96-amino acid, threonine-rich, heavily O-glycosylated (80-90% by weight) glycoprotein with a single transmembrane segment at its C-terminus and no apparent cytosolic domain. Of 12 extracellular Thr residues, eight are O-glycosylated and three are non-glycosylated. Flagellasialin is highly expressed in testis, but cannot be detected in ovary. The amino acid sequences of flagellasialin from three sea urchin species (H. pulcherrimus, Strongylocentrotus purpuratus and S. franciscanus) are identical, but some species differences exist in the three core glycan structures to which the sulfated α2,9-linked polyNeu5Ac chain is linked. Finally, a specific antibody against the α2,9-linked polyNeu5Ac structure inhibits sperm motility and reduces fertilization, implicating flagellasialin as a regulator of these critical processes.

DOI： 10.1093/glycob/cwl036

*Identification of disialic acid-containing glycoproteins in mouse serum. 査読有り

Yasukawa, Z., Sato, C., Sano, K., Ogawa, H. and Kitajima, K.

Glycobiology 15 巻頁： 827-837 2006年

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

Serum glycoproteins are involved in various biological activities such as removal of exogenous antigen, fibrinolysis, transport of metal. They are also useful markers of inflammation and diseases. Although it is known that the amount of sialic acid increases after inflammation, little attention has been paid to the presence of linkage specific epitope in serum,especially, the α2,8-linkage. In the previous study, we demonstrated that four components in mouse serum contain the α2,8-linked disialic acid, based on the immunoreactivity with mAb.2-4B that is specific to Neu5Gcα2→(8Neu5Gcα2→)n-1, n≥2 (Yasukawa, Z. et al. (2005) Glycobiology 15, 827-837). In this study, we purified three components, 30kDa-gp, 70kDa-gp, and 120kDa-gp and identified as the light chain of immunoglobulins, vitronectin, and plasminogen, respectively, using MALDI-TOF MS analyses. The modifications of these proteins with the α2,8-linked disialic acid was chemically confirmed by fluorometric C7/C9 analyses and acid hydrolysates-DMB methods. We also showed that the light chains of IgG, IgM and IgE are commonly modified with the α2,8-linked disialic acid. In addition, not only mouse vitronectin but also rat vitronectin was also modified with disialic acid and the amount of disialylation on vitronectin decreased after hepatectomy. These results indicate that disialic acid-modification on serum glycoprotein has some biological meanings in immunological events and fibrinolysis.

Involvement of the alpha2,8-polysialyltransferases II/STX and IV/PST in the biosynthesis of polysialic acid chains on the O-linked glycoproteins in rainbow trout ovary. 査読有り

Asahina S, Sato C, Matsuno M, Matsuda T, Colley K, Kitajima K. Asahina S, Sato C, Matsuno M, Matsuda T, Colley K, Kitajima K.

J Biochem. 140 巻頁： 687-701 2006年

Oral ingestion of mannose alters the expression level of deaminoneuraminic acid (KDN) in mouse organs. 査読有り

Go, S., Sato, C., and Kitajima, K.

Glycoconjugate J. 23 巻頁： 411-421 2006年

Weaning-induced expression of a milk-fat globule protein, MFG-E8, in mouse mammary glands, as demonstrated by the analyses of its mRNA, protein and phosphatidylserine-binding activity. 査読有り Open Access

Nakatani H, Aoki N, Nakagawa Y, Jin-No S, Aoyama K, Oshima K, Ohira S, Sato C, Nadano D, Matsuda T.

Biochem J. 395 巻頁： 21-30 2006年

Nakatani H, Aoki N, Nakagawa Y, Jin-No S, Aoyama K, Oshima K, Ohira S, Sato C, Nadano D, Matsuda T

Biochem J. 395 巻頁： 21-30 2006年

Hypoxic culture induces expression of Sialin, a sialic acid transporter, and cancer-associated gangliosides containing non-human sialic acid on human cancer cells. , 査読有り Open Access

Yin, J, Hashimoto, A, Izawa, M, Miyazaki, K, Chen G-Y, Takematsu, H, Kozutsumi, Y, Suzuki, A, Furuhata, K, Cheng, F.-L. Lin, C.-H, Sato, C, Kitajima, K, Kannagi, R

Cancer Res. 66 巻頁： 2937-2945 2006年

Oral ingestion of mannose alters the expression level of deaminoneuraminic acid (KDN) in mouse organs. 査読有り

Go, S, Sato, C, Kitajima, K

Glycoconjugate J. 23 巻頁： 411-421 2006年

Involvement of the alpha2,8-polysialyltransferases II/STX and IV/PST in the biosynthesis of polysialic acid chains on the O-linked glycoproteins in rainbow trout ovary. 査読有り

Asahina S, Sato C, Matsuno M, Matsuda T, Colley K, Kitajima, K, Asahina S, Sato C, Matsuno M, Matsuda T, Colley K, Kitajima K

J Biochem. 140 巻頁： 687-701 2006年

Inflammation-dependent changes in alpha2,3-, alpha2,6-, and alpha2,8-sialic acid glycotopes on serum glycoproteins in mice. 査読有り Open Access

Yasukawa Z, Sato C, Kitajima K

Glycobiology 15 巻 ( 9 ) 頁： 827 - 37 2005年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Glycobiology

The expression of acute-phase serum proteins increases in response to inflammatory stimuli. Most of these proteins are glycoproteins that often contain sialic acids (Sia). It is unknown, however, how the expression of Sia in these glycoproteins changes during inflammation. This study demonstrates changes in the α2,3-, α2,6-, and α2,8-Sia glycotopes on serum glycoproteins in response to turpentine oil-induced inflammation, based on lectin- and immunoblot analyses by using sialyl linkage-specific lectins, Maackia amurensis for the α2,3-Sia glycotope and Sambucus sieboldiana for the α2,6-Sia glycotopes, and monoclonal antibody 2-4B (mAb.2-4B) recognizing the di- and oligomers of the α2,8-Neu5Gc residue. There was an increase in a limited number of sialoglycoproteins containing the α2,3-, α2,6-, or α2,8-Sia glycotopes. Reverse transcription-polymerase chain reaction (RT-PCR) analysis of the expression profiles of mRNAs for the known sialyltransferases in mouse liver during inflammation indicated the up-regulated expression of β-galactoside α2,3-sialyltransferases (ST3Gal I and ST3Gal III) and β-N-acetylgalactosaminide α2,6-sialyltransferase (ST6GalNAc VI) as well as β-galactoside α2,6-sialyltransferase (ST6Gal I) mRNAs. Notably, ST3Gal I and III and ST6GalNAc VI are involved in the synthesis of the α2,3- and α2,6-Sia glycotopes on O-glycan chains and possibly on gangliosides, whereas ST6Gal I is specific for N-glycan chains. These results provide evidence for the inflammation-induced expression of sialyl glycotopes in serum glycoproteins. We demonstrated that inflammation significantly increased the expression of an unknown 32-kDa glycoprotein containing the α2,8-Sia glycotope. The mechanism for the increase in glycoprotein in inflamed mouse serum remains to be examined, as mRNA expression for all of the α2,8-sialyltransferases (ST8Sia I-VI) was unchanged during inflammation. © The Author 2005. Published by Oxford University Press. All right reserved.

DOI： 10.1093/glycob/cwi068

<佐藤 ちひろ>

論文 - 佐藤 ちひろ

<佐藤　ちひろ>

論文 - 佐藤　ちひろ