研究者詳細 - 佐藤　ちひろ

論文 - 佐藤　ちひろ

分割表示 401 件中 201 - 220 件目／全件表示 >>

.The AMOR Arabinogalactan Sugar Chain Induces Pollen-Tube Competency to Respond to Ovular Guidance. 査読有り Open Access

Mizukami AG, Inatsugi R, Jiao J, Kotake T, Kuwata K, Ootani K, Okuda S, Sankaranarayanan S, Sato Y, Maruyama D, Iwai H, Garénaux E, Sato C, Kitajima K, Tsumuraya Y, Mori H, Yamaguchi J, Itami K, Sasaki N, Higashiyama T

Curr Biol. 26 巻 ( 8 ) 頁： 1091-7 2016年4月

2,11-Dibromo-13,14-dimesityl-5,8-dioxapentaphene: A Stable and Twisted Polycyclic System Containing the o-Quinodimethane Skeleton.

Sato C, Suzuki S, Kozaki M, Okada K

Organic letters 18 巻 ( 5 ) 頁： 1052 - 5 2016年3月

Flagellasialin: Highly Glycosylated Gpi-Anchored Protein Involved in Intracellular Ca2+ Regulation in Sea Urchin Sperm 査読有り

Kanazawa T., Suzuki E., Miyata S., Sato C., Kitajima K.

Glycoscience Biology and Medicine 頁： 883 - 890 2015年1月

記述言語：英語掲載種別：論文集(書籍)内論文出版者・発行元：Glycoscience Biology and Medicine

Very little knowledge has been available on structure and function of heavily glycosylated proteins, whose glycan parts amount to more than 90 % of the whole molecules, mainly because of technical difficulties in detection and determination of structure. In 2004 flagellasialin was discovered as a major cell surface component in the lipid rafts of sea urchin sperm. The extremely high content of glycan chains completely prevented detecting this glycoprotein by conventional protein staining methods, such as Coomassie Brilliant Blue and silver staining. To make this glycoprotein visible, monoclonal antibodies 4F7 and 3G9 were developed as specific probes for detecting glycan chains of flagellasialin. The frequent occurrence of GPI-anchored proteins in lipid rafts is well recognized. Flagellasialin was actually demonstrated to contain a GPI-anchor, based on three lines of experiments. In addition to the computational predictions from the cDNA sequence and the phosphatidylinositolspecific phospholipase C treatment of the sperm membrane fraction, nitrous acid deamination of a flagellasialin-blotted membrane was newly introduced. The immunostaining of flagellasialin on the membrane disappeared after the nitrous acid treatment of the blotted membrane. This method was easy and effective for identifying flagellasialin as a GPI-anchored protein.

DOI： 10.1007/978-4-431-54841-6_167

Scopus

Polysialyltransferase assay 査読有り

Hane M., Kitajima K., Sato C.

Glycoscience Biology and Medicine 頁： 529 - 534 2015年1月

記述言語：英語掲載種別：論文集(書籍)内論文出版者・発行元：Glycoscience Biology and Medicine

It has been shown that polysialic acid (polySia) is an indispensable component for viability of mouse during development. Especially, it is deeply involved in normal brain function in mouse and human, affecting cell adhesion, cell migration, cell growth, neurogenesis, and synaptogenesis. PolySia is synthesized by two polysialyltransferases, ST8SIA2 and ST8SIA4. Mice deficient in both enzymes show lethality soon after birth. Mice deficient in either ST8SIA2 or ST8SIA4 show significant impairments in learning, memory, circadian rhythm, and social behavior. Interestingly, different phenotypes are often observed between ST8SIA2- and ST8SIA4-deficient mice. Therefore, characterization of these enzymes in vitro and in vivo and precise understanding of the structurefunction relationship of polySia become more and more important. In this chapter, assay procedures for in vitro activity of the polysialyltransferases are focused on to obtain clear results of their in vitro activities that are often said to be very weak compared with other sialyltransferases.

DOI： 10.1007/978-4-431-54841-6_118

Scopus

Polysialic acid 査読有り

Sato C., Kitajima K.

Glycoscience Biology and Medicine 頁： 519 - 528 2015年1月

記述言語：英語掲載種別：論文集(書籍)内論文出版者・発行元：Glycoscience Biology and Medicine

Polysialic acid (polySia), particularly α2,8-linked polyNeu5Ac (DP ¼ 8 ~ 400), is a unique polymer of sialic acid (Sia) and modifies neural cell adhesion molecule (NCAM) spatiotemporally in embryonic brains. PolySia is involved in cell migration, neural outgrowth, axonal guidance, synaptic plasticity, and the development of normal neural circuits and neurogenesis due to its anti- adhesive property and spatiotemporal expression. Recent improvements of polySia-detection methods have told us that polySia expression persists in certain areas of the adult brain, such as the olfactory bulb, hippocampus, subventricular zone, thalamus, prefrontal cortex, and amygdala, where neural plasticity, remodeling of neural connections, or neural generation is ongoing. Importantly, several lines of evidence have raised a new concept of polySia function that polySia works as an attractive field that associates with particular ion channels and neurologically active molecules, such as neurotrophic factor (BDNF), growth factor (FGF2), and neurotransmitter (dopamine), to regulate their involved signaling. It has been shown that polySia is involved in learning, memory, circadian rhythm, and social behaviors using polySia-impaired mice. In addition, SNPs and some deletions in genes encoding the polysialyltransferase and NCAM have been reported from patients of schizophrenia, mood disorder and autism. Such impairments of polySia structure derived from polysialyltransferase gene alterations might influence the polySia function not only as the repulsive field but also as the attractive field.

DOI： 10.1007/978-4-431-54841-6_117

Scopus

Protective effects of polysialic acid on proteolytic cleavage of FGF2 and proBDNF/BDNF. 査読有り Open Access

Hane M, Matsuoka S, Ono S, Miyata S, Kitajima K, Sato C.

Glycobiology 25 巻頁： 1112-24 2015年

Sulfatide-Hsp70 interaction promotes Hsp70 clustering and stabilizes binding to unfolded protein. 査読有り

Harada Y, Sato C, Kitajima K.

Biomolecules. 5 巻頁： 958-73 2015年

Ganglioside contained in the neuronal tissue-enriched acidic protein of 22 kDa (NAP-22) fraction prepared from the detergent-resistant membrane microdomain of rat brain inhibits the phosphatase activity of calcineurin. 査読有り

Kobayashi Y, da Silva R, Kumanogoh H, Miyata S, Sato C, Kitajima K, Nakamura S, Morita M, Hayashi F, Maekawa S.

J Neurosci Res. 93 巻頁： 1462-70 2015年

Rapid Trimming of Cell Surface Polysialic Acid (PolySia) by Exovesicular Sialidase Triggers Release of Preexisting Surface Neurotrophin. 査読有り Open Access

Sumida M, Hane M, Yabe U, Shimoda Y, Pearce OM, Kiso M, Miyagi T, Sawada M, Varki A, Kitajima K, Sato C.

J Biol Chem. 290 巻頁： 13202-14 2015年

Discovery, primary, and crystal structures and capacitation-related properties of a prostate-derived heparin-binding protein WGA16 from boar sperm. 査読有り Open Access

Garénaux E, Kanagawa M, Tsuchiyama T, Hori K, Kanazawa T, Goshima A, Chiba M, Yasue H, Ikeda A, Yamaguchi Y, Sato C, Kitajima K.

J Biol Chem. 290 巻頁： 5484-501 2015年

Sulfatide-Hsp70 interaction promotes Hsp70 clustering and stabilizes binding to unfolded protein. 査読有り

Harada Y, Sato C, Kitajima K

Biomolecules. 5 巻頁： 958-73 2015年

Discovery, primary, and crystal structures and capacitation-related properties of a prostate-derived heparin-binding protein WGA16 from boar sperm. 査読有り Open Access

Garénaux E, Kanagawa M, Tsuchiyama T, Hori K, Kanazawa T, Goshima A, Chiba M, Yasue H, Ikeda A, Yamaguchi Y, Sato C, Kitajima K

J Biol Chem. 290 巻頁： 5484-501 2015年

Advanced Technologies in Sialic Acid and Sialoglycoconjugate Analysis. 査読有り Open Access

Kitajima K, Varki N, Sato C

Topics in current chemistry 367 巻頁： 75 - 103 2015年

記述言語：英語掲載種別：論文集(書籍)内論文出版者・発行元：Topics in Current Chemistry

Although the structural diversity of sialic acid (Sia) is rapidly expanding, understanding of its biological significance has lagged behind. Advanced technologies to detect and probe diverse structures of Sia are absolutely necessary not only to understand further biological significance but also to pursue medicinal and industrial applications. Here we describe analytical methods for detection of Sia that have recently been developed or improved, with a special focus on 9-O-acetylated N-acetylneuraminic acid (Neu5,9Ac), N-glycolylneuraminic acid (Neu5Gc), deaminoneuraminic acid (Kdn),O-sulfated Sia (SiaS), and di-, oligo-, and polysialic acid (diSia/oligoSia/polySia) in glycoproteins and glycolipids. Much more attention has been paid to these Sia and sialoglycoconjugates during the last decade, in terms of regulation of the immune system, neural development and function, tumorigenesis, and aging.

DOI： 10.1007/128_2013_458

Web of Science

Scopus

PubMed

Kobayashi Y, da Silva R, Kumanogoh H, Miyata S, Sato C, Kitajima K, Nakamura S, Morita M, Hayashi F, Maekawa S

J Neurosci Res. 93 巻頁： 1462-70 2015年

Rapid Trimming of Cell Surface Polysialic Acid (PolySia) by Exovesicular Sialidase Triggers Release of Preexisting Surface Neurotrophin. 査読有り Open Access

Sumida M, Hane M, Yabe U, Shimoda Y, Pearce OM, Kiso M, Miyagi T, Sawada M, Varki A, Kitajima K, Sato C

J Biol Chem. 290 巻頁： 13202-14 2015年

Protective effects of polysialic acid on proteolytic cleavage of FGF2 and proBDNF/BDNF. 査読有り Open Access

Hane M, Matsuoka S, Ono S, Miyata S, Kitajima K, Sato C

Glycobiology 25 巻頁： 1112-24 2015年

Tissue-specific posttranslational modification allows functional targeting of thyrotropin. 査読有り Open Access

Ikegami K, Liao XH, Hoshino Y, Ono H, Ota W, Ito Y, Nishiwaki-Ohkawa T, Sato C, Kitajima K, Iigo M, Shigeyoshi Y, Yamada M, Murata Y, Refetoff S, Yoshimura T

Cell reports 9 巻 ( 3 ) 頁： 801 - 10 2014年11月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Cell Reports

Thyroid-stimulating hormone (TSH; thyrotropin) is a glycoprotein secreted from the pituitary gland. Pars distalis-derived TSH (PD-TSH) stimulates the thyroid gland to produce thyroid hormones (THs), whereas pars tuberalis-derivedTSH(PT-TSH) actsonthe hypothalamus to regulate seasonal physiology and behavior. However, it had not been clear how these two TSHs avoid functional crosstalk. Here, we show that this regulation ismediated by tissue-specific glycosylation. AlthoughPT-TSHis releasedinto the circulation, it does not stimulate the thyroid gland. PD-TSH is known to have sulfated biantennary N-glycans, and sulfated TSH is rapidly metabolized in the liver. In contrast, PT-TSH has sialylated multibranched N-glycans; in the circulation, it forms the macro-TSH complex with immunoglobulin or albumin, resulting in the loss of its bioactivity. Glycosylation is fundamental to awide range of biological processes. This report demonstrates its involvement in preventing functional crosstalk of signaling molecules in the body.

DOI： 10.1016/j.celrep.2014.10.006

Interaction of 70-kDa heat shock protein with glycosaminoglycans and acidic glycopolymers. 査読有り Open Access

Harada Y, Garenáux E, Nagatsuka T, Uzawa H, Nishida Y, Sato C, Kitajima K

Biochemical and biophysical research communications 453 巻 ( 2 ) 頁： 229 - 34 2014年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Biochemical and Biophysical Research Communications

Interaction of Hsp70 with natural and artificial acidic glycans is demonstrated based on the native PAGE analysis. Hsp70 interacts with acidic glycopolymers that contain clustered sulfated and di-sialylated glycan moieties on a polyacrylamide backbone, but not with neutral or mono-sialylated glycopolymers. Hsp70 also interacts and forms a large complex with heparin, heparan sulfate, and dermatan sulfate that commonly contain 2-O-sulfated iduronic acid residues, but not with other types of glycosaminoglycans (GAGs). Hsp70 consists of the N-terminal ATPase domain and the C-terminal peptide-binding domain. The interaction analyses using the recombinant N- and C-terminal half domains show that the ATPase domain mediates the direct interaction with acidic glycans, while the peptide-binding domain stabilizes the large complexes with particular GAGs. To our knowledge, this is the first demonstration of direct binding of Hsp70 to the particular GAGs. This property may be involved in the physiological functions of Hsp70 at the plasma membrane and extracellular environments.

DOI： 10.1016/j.bbrc.2014.05.137

Web of Science

Scopus

PubMed

ST8 alpha-N-acetyl-neuraminide alpha- 2,8-sialyltransferase 2 (ST8SIA2)

Sato C.

Handbook of Glycosyltransferases and Related Genes, Second Edition 2 巻頁： 781-795 2014年1月

Polysialic acid: Biosynthesis, novel functions and applications. 査読有り

Colley K., Kitajima K, Sato C.

Critical Reviews in Biochemistry and Molecular Biology 頁： 1-35 2014年

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