研究者詳細 - 佐藤　ちひろ

論文 - 佐藤　ちひろ

分割表示 401 件中 101 - 120 件目／全件表示 >>

Oral ingestion of mannose alters the expression level of deaminoneuraminic acid (KDN) in mouse organs.

Go S, Sato C, Furuhata K, Kitajima K

Glycoconjugate journal 23 巻 ( 5-6 ) 頁： 411 - 21 2006年7月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Glycoconjugate Journal

Deaminoneuraminic acid (KDN) is a unique member of the sialic acid family. We previously demonstrated that free KDN is synthesized de novo from mannose as its precursor sugar in trout testis, and that the amount of intracellular KDN increases in mouse B16 melanoma cells cultured in mannose-rich media [Angata et al. (1999) J. Biol. Chem. 274, 22949-56; Angata et al. (1999) Biochem. Biophys. Res. Commun. 261, 326-31]. In the present study, we first demonstrated a mannose-induced increase in intracellular KDN in various cultured mouse and human cell lines. These results led us to examine whether KDN expression in mouse organs is altered by exogenously administered mannose. Under normal feeding conditions, intracellular free KDN was present at very low levels (19-48 pmol/mg protein) in liver, spleen, and lung, and was not detected in kidney or brain. Oral ingestion of mannose, both short-term (90 min) and long-term (2 wk), resulted in an increase of intracellular KDN up to 60-81 pmol/mg protein in spleen and lung and 6.9-18 pmol/mg protein in kidney and brain; however, no change was observed in liver. The level of KDN in organs appears not to be determined only by the KDN 9-phosphate synthase activity, but might also be affected by other enzymes that utilize mannose 6-phosphate as a substrate as well as the enzymes that breakdown KDN, like KDN-pyruvate lyase. In blood, the detectable amount of free KDN did not change on oral ingestion of mannose. These findings indicate that mannose in the diet affects KDN metabolism in various organs, and provide clues to the mechanism of altered KDN expression in some tumor cells and aged organs. © Springer Science + Business Media, LLC 2006.

DOI： 10.1007/s10719-006-6734-z

Web of Science

Scopus

PubMed

Weaning-induced expression of a milk-fat globule protein, MFG-E8, in mouse mammary glands, as demonstrated by the analyses of its mRNA, protein and phosphatidylserine-binding activity. Open Access

Nakatani H, Aoki N, Nakagawa Y, Jin-No S, Aoyama K, Oshima K, Ohira S, Sato C, Nadano D, Matsuda T

The Biochemical journal 395 巻 ( 1 ) 頁： 21 - 30 2006年4月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Biochemical Journal

A milk membrane glycoprotein, MFG-E8 [milk fat globule-EGF (epidermal growth factor) factor 8], is expressed abundantly in lactating mammary glands in stage- and tissue-specific manners, and has been believed to be secreted in association with milk fat globules. In the present paper, we describe further up-regulation of MFG-E8 in involuting mammary glands, where the glands undergo a substantial increase in the rate of epithelial cell apoptosis, and a possible role of MFG-E8 in mediating recognition and engulfment of apoptotic cells through its specific binding to PS (phosphatidylserine). Immunoblotting and RNA blotting analyses revealed that both MFG-E8 protein and MFG-E8 mRNA were markedly increased in mammary tissue within 3 days of either natural or forced weaning (pup withdrawal) of lactating mice. Using immunohistochemical analysis of the mammary tissue cryosections, the MFG-E8 signal was detected around the epithelium of such involuting mammary glands, but was almost undetectable at early- and mid-lactation stages, although strong signals were obtained for milk fat globules stored in the alveolar lumen. Some signals double positive to a macrophage differentiation marker, CD68, and MFG-E8 were detected in the post-weaning mammary tissue, although such double-positive signals were much smaller in number than the MFG-E8 single-positive ones. Total MFG-E8 i n milk was also increased in the post-weaning mammary glands and, furthermore, the free MFG-E8 content in the post-weaning milk, as measured by in vitro PS-binding and apoptotic HC11 cell-binding activities, was much higher than that of lactation. In addition, the post-weaning milk enhanced the binding of apoptotic HC11 cells to J774 macrophages. Sucrose density-gradient ultracentrifugation analyses revealed that such enhanced PS-binding activity of MFG-E8 was present in membrane vesicle fractions (density 1.05-1.13 g/ml), rather than milk fat globule fractions. The weaning-induced MFG-E8 might play an important role in the recognition and engulfment of apoptotic epithelial cells by the neighbouring phagocytic epithelial cells in involuting mammary glands. © 2006 Biochemical Society.

DOI： 10.1042/BJ20051459

Hypoxic culture induces expression of sialin, a sialic acid transporter, and cancer-associated gangliosides containing non-human sialic acid on human cancer cells Open Access

Yin, J; Hashimoto, A; Izawa, M; Miyazaki, K; Chen, GY; Takematsu, H; Kozutsumi, Y; Suzuki, A; Furuhata, K; Cheng, FL; Lin, CH; Sato, C; Kitajima, K; Kannagi, R

CANCER RESEARCH 66 巻 ( 6 ) 頁： 2937 - 2945 2006年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Cancer Research

Tumor hypoxia figures heavily in malignant progression by altering the intracellular glucose metabolism and inducing angiogenic factor production, thus, selecting and expanding more aggressive cancer cell clones. Little is known, however, regarding hypoxia-induced antigenic changes in cancers. We investigated the expression of N-glycolyl sialic acid (NeuGc)-G<inf>M2</inf>, a cancer-associated ganglioside containing non-human sialic acid, NeuGc, in human cancers. Cancer tissues prepared from patients with colon cancers frequently expressed NeuGc-G<inf>M2</inf>, whereas it was virtually absent in nonmalignant colonic epithelia. Studies on cultured cancer cells indicated that the non-human sialic acid was incorporated from culture medium. Hypoxic culture markedly induced mRNA for a sialic acid transporter, sialin, and this accompanied enhanced incorporation of NeuGc as well as N-acetyl sialic acid. Transfection of cells with sialin gene conferred accelerated sialic acid transport and induced cell surface expression of NeuGc-G<inf>M2</inf>. We propose that the preferential expression of NeuGc-G<inf>M2</inf> in cancers is closely associated with tumor hypoxia. Hypoxic culture of tumor cells induces expression of the sialic acid transporter, and enhances the incorporation of non-human sialic acid from the external milieu. A consequence of this is the acquisition of cancer-associated cell surface gangliosides, typically G<inf>M2</inf>, containing non-human sialic acid (NeuGc), which is not endogenously synthesized through CMP-N-acetyl sialic acid hydroxylase because humans lack the gene for the synthetic enzyme. As hypoxia is associated with diminished response to radiotherapy and chemotherapy, NeuGc-G<inf>M2</inf> is a potential therapeutic target for hypoxic cancer cells. ©2006 American Association for Cancer Research.

DOI： 10.1158/0008-5472.CAN-05-2615

AN N-TERMINAL 130 KDA FRAGMENT OF A FOURTH SEA URCHIN SPERM RECEPTOR FOR EGG JELLY MODULE PROTEIN, REJ4, IS SECRETED DURING ACROSOME REACTION(Developmental Biology,Abstracts of papers presented at the 76^<th> Annual Meeting of the Zoological Society of Japan) : Open Access

MiyataShinji, SatoChihiro, ToriyamaMasaru, KitajamaKen

Zoological science 22 巻 ( 12 ) 頁： 1456 2005年12月

CAPACITATION-INDUCED CHANGES OF THE SURFACE DISTRIBUTION OF SIALOGLYCOTOPES ON PIG SPERM(Developmental Biology,Abstracts of papers presented at the 76^<th> Annual Meeting of the Zoological Society of Japan) : Open Access

XueLian, MiyataShinji, SatoChihiro, KitajimaKen

Zoological science 22 巻 ( 12 ) 頁： 1456 2005年12月

An N-terminal 130 kDa fragment of a fourth sea urchin sperm receptor for egg jelly module protein, REJ4, is secreted during acrosome reaction

Miyata Shinji, Sato Chihiro, Toriyama Masaru, Kitajama Ken

ZOOLOGICAL SCIENCE 22 巻 ( 12 ) 頁： 1456 - 1456 2005年12月

Capacitation-induced changes of the surface distribution of sialoglycotopes on pig sperm

Xue Lian, Miyata Shinji, Sato Chihiro, Kitajima Ken

ZOOLOGICAL SCIENCE 22 巻 ( 12 ) 頁： 1456 - 1456 2005年12月

5.糖鎖と発生、4)ポリシアル酸

佐藤ちひろ

遺伝子医学(糖鎖と病気)MOOK 3 頁： 186 - 191 2005年

A MAJOR GANGLIOSIDE FROM HEMICENTROTUS PULCHERRIMUS SPERM SPECIFICALLY INTERACTS WITH PHOSPHOLIPIDS(Developmental Biology,Abstracts of papers presented at the 75^<th> Annual Meeting of the Zoological Society of Japan) : Open Access

YamakawaNao, SatoChihiro, KitajimaKen

Zoological science 21 巻 ( 12 ) 頁： 1292 2004年12月

CELL GROWTH STIMULATING ACTIVITY OF THE PEPTIDES DERIVED FROM CORTICAL ALVEOLUS GLYCOPROTEINS OF FISH EGG (HYOSOPHORIN)(Developmental Biology,Abstracts of papers presented at the 75^<th> Annual Meeting of the Zoological Society of Japan) : Open Access

MaedaEri, SatoChihiro, KitajimaKen

Zoological science 21 巻 ( 12 ) 頁： 1292 2004年12月

THE 130 KDA GLYCOPROTEIN IS SECRETED FROM SPERM DURING ACROSOME REACTION OF SEA URCHIN, HEMICENTROTUS PULCHERRIMUS(Developmental Biology,Abstracts of papers presented at the 75^<th> Annual Meeting of the Zoological Society of Japan) : Open Access

MiyataShinji, SatoChihiro, ToriyamaMasaru, HirohashiNoritaka, VacquierVictor, KitajimaKen

Zoological science 21 巻 ( 12 ) 頁： 1292 2004年12月

A major ganglioside from Hemicentrotus pulcherrimus sperm specifically interacts with phospholipids

Yamakawa Nao, Sato Chihiro, Kitajima Ken

ZOOLOGICAL SCIENCE 21 巻 ( 12 ) 頁： 1292 - 1292 2004年12月

Cell growth stimulating activity of the peptides derived from cortical alveolus glycoproteins of fish egg (hyosophorin)

Maeda, E; Sato, C; Kitajima, K

ZOOLOGICAL SCIENCE 21 巻 ( 12 ) 頁： 1292 - 1292 2004年12月

The 130 kDa glycoprotein is secreted from sperm during acrosome reaction of sea urchin, Hemicentrotus pulcherrimus

Miyata Shinji, Sato Chihiro, Toriyama Masaru, Hirohashi Noritaka, Vacquier Victor, Kitajima Ken

ZOOLOGICAL SCIENCE 21 巻 ( 12 ) 頁： 1292 - 1292 2004年12月

A newly identified zona pellucida glycoprotein, ZPD, and dimeric ZP1 of chicken egg envelope are involved in sperm activation on sperm-egg interaction. Open Access

Okumura H, Kohno Y, Iwata Y, Mori H, Aoki N, Sato C, Kitajima K, Nadano D, Matsuda T

The Biochemical journal 384 巻 ( Pt 1 ) 頁： 191 - 9 2004年11月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Biochemical Journal

Fertilization begins with interaction between the sperm and the egg. The surface of the vertebrate oocyte is covered with the egg envelope, which is composed of ZP (zona pellucida) glycoproteins. We have identified two glycoproteins, ZP1/gp97 and ZPC/gp42, as the major components of the chicken egg envelope. In the present study, another 42 kDa protein, designated ZPD, has been found as a new major component of the chicken egg envelope. ZPD was specifically released from the egg envelope by ultrasonication treatment without urea. ZPD cDNA was cloned using a chicken granulosa cell cDNA pool. The deduced amino acid sequence showed that preproprotein of ZPD is composed of 418 amino acid residues with four potential N-glycosylation sites and includes a ZP domain, common in vertebrate ZP glycoproteins, and a transmembrane domain. ZPD belongs phylogenetically to a distinct group from known ZP glycoprotein subfamilies, ZPA, ZPB, and ZPC. In two-dimensional gel electrophoresis ZPD proteins were identified to be several isoforms with different pi values between 5 and 7. ZP1, ZPC and the newly identified ZPD were confirmed to be the major components of chicken egg envelope by MS of proteolytic digests of whole egg envelope. The in vitro incubation of chicken sperm with calcium ionophore A23187 induced sperm activation, resulting in the fragmentation and release of a 41 kDa PNA (peanut agglutinin)-positive glyco-protein and the decrease or loss of sperm PNA-stainability. The incubation with ZPD and dimeric ZP1, but not ZPC and monomeric ZPl, also induced the decrease or loss of sperm PNA-stainability, suggesting the in vitro sperm activation by these ZP components. Collectively, ZPD might bind loosely to egg envelope matrix and play a key role in the sperm activation on avian sperm-egg interaction.

DOI： 10.1042/BJ20040299

Development of sensitive chemical and immunochemical methods for detecting sulfated sialic acids and their application to localization and quantitation studies of sea urchin sperm and eggs

Yamakawa N, Maehashi E, Miyata S, Sato C, Furuhata K, Kitajima K

GLYCOBIOLOGY 14 巻 ( 11 ) 頁： 1146 - 1146 2004年11月

Effects of exogenously administered mannose and deaminoneuraminic acid (KDN) to mice or murine cultured cells on the metabolism of KDN

Go S, Sato C, Kitajima K

GLYCOBIOLOGY 14 巻 ( 11 ) 頁： 1166 - 1167 2004年11月

Mouse heat shock protein 70 recognizes negatively charged glycolipids and phospholipids

Harada Y, Sato C, Kitajima K

GLYCOBIOLOGY 14 巻 ( 11 ) 頁： 1101 - 1102 2004年11月

Occurrence and characterization of a novel sulfated alpha 2,9-linked polysialic acid-containing glycoprotein in sea urchin sperm flagellum

Miyata S, Sato C, Kitajima K

GLYCOBIOLOGY 14 巻 ( 11 ) 頁： 1146 - 1146 2004年11月

Mouse T cell CD166 is a disialic acid-containing glycoprotein and involved in T cell activation

Sato C, Nohara K, Matsuda T, Kitajima K

GLYCOBIOLOGY 14 巻 ( 11 ) 頁： 1136 - 1136 2004年11月

　前のページ - 次のページ　

<佐藤 ちひろ>

論文 - 佐藤 ちひろ

<佐藤　ちひろ>

論文 - 佐藤　ちひろ