研究者詳細 - 佐藤　ちひろ

論文 - 佐藤　ちひろ

分割表示 376 件中 181 - 200 件目／全件表示 >>

Sulfatide-Hsp70 interaction promotes Hsp70 clustering and stabilizes binding to unfolded protein. 査読有り

Harada Y, Sato C, Kitajima K

Biomolecules. 5 巻頁： 958-73 2015年

Advanced Technologies in Sialic Acid and Sialoglycoconjugate Analysis. 査読有り

Kitajima K, Varki N, Sato C

Topics in current chemistry 367 巻頁： 75 - 103 2015年

記述言語：英語掲載種別：論文集(書籍)内論文出版者・発行元：Topics in Current Chemistry

Although the structural diversity of sialic acid (Sia) is rapidly expanding, understanding of its biological significance has lagged behind. Advanced technologies to detect and probe diverse structures of Sia are absolutely necessary not only to understand further biological significance but also to pursue medicinal and industrial applications. Here we describe analytical methods for detection of Sia that have recently been developed or improved, with a special focus on 9-O-acetylated N-acetylneuraminic acid (Neu5,9Ac), N-glycolylneuraminic acid (Neu5Gc), deaminoneuraminic acid (Kdn),O-sulfated Sia (SiaS), and di-, oligo-, and polysialic acid (diSia/oligoSia/polySia) in glycoproteins and glycolipids. Much more attention has been paid to these Sia and sialoglycoconjugates during the last decade, in terms of regulation of the immune system, neural development and function, tumorigenesis, and aging.

DOI： 10.1007/128_2013_458

Web of Science

Scopus

PubMed

Discovery, primary, and crystal structures and capacitation-related properties of a prostate-derived heparin-binding protein WGA16 from boar sperm. 査読有り

Garénaux E, Kanagawa M, Tsuchiyama T, Hori K, Kanazawa T, Goshima A, Chiba M, Yasue H, Ikeda A, Yamaguchi Y, Sato C, Kitajima K

J Biol Chem. 290 巻頁： 5484-501 2015年

Rapid Trimming of Cell Surface Polysialic Acid (PolySia) by Exovesicular Sialidase Triggers Release of Preexisting Surface Neurotrophin. 査読有り

Sumida M, Hane M, Yabe U, Shimoda Y, Pearce OM, Kiso M, Miyagi T, Sawada M, Varki A, Kitajima K, Sato C

J Biol Chem. 290 巻頁： 13202-14 2015年

Protective effects of polysialic acid on proteolytic cleavage of FGF2 and proBDNF/BDNF. 査読有り

Hane M, Matsuoka S, Ono S, Miyata S, Kitajima K, Sato C

Glycobiology 25 巻頁： 1112-24 2015年

Ganglioside contained in the neuronal tissue-enriched acidic protein of 22 kDa (NAP-22) fraction prepared from the detergent-resistant membrane microdomain of rat brain inhibits the phosphatase activity of calcineurin. 査読有り

Kobayashi Y, da Silva R, Kumanogoh H, Miyata S, Sato C, Kitajima K, Nakamura S, Morita M, Hayashi F, Maekawa S

J Neurosci Res. 93 巻頁： 1462-70 2015年

Tissue-specific posttranslational modification allows functional targeting of thyrotropin. 査読有り Open Access

Ikegami K, Liao XH, Hoshino Y, Ono H, Ota W, Ito Y, Nishiwaki-Ohkawa T, Sato C, Kitajima K, Iigo M, Shigeyoshi Y, Yamada M, Murata Y, Refetoff S, Yoshimura T

Cell reports 9 巻 ( 3 ) 頁： 801 - 10 2014年11月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Cell Reports

Thyroid-stimulating hormone (TSH; thyrotropin) is a glycoprotein secreted from the pituitary gland. Pars distalis-derived TSH (PD-TSH) stimulates the thyroid gland to produce thyroid hormones (THs), whereas pars tuberalis-derivedTSH(PT-TSH) actsonthe hypothalamus to regulate seasonal physiology and behavior. However, it had not been clear how these two TSHs avoid functional crosstalk. Here, we show that this regulation ismediated by tissue-specific glycosylation. AlthoughPT-TSHis releasedinto the circulation, it does not stimulate the thyroid gland. PD-TSH is known to have sulfated biantennary N-glycans, and sulfated TSH is rapidly metabolized in the liver. In contrast, PT-TSH has sialylated multibranched N-glycans; in the circulation, it forms the macro-TSH complex with immunoglobulin or albumin, resulting in the loss of its bioactivity. Glycosylation is fundamental to awide range of biological processes. This report demonstrates its involvement in preventing functional crosstalk of signaling molecules in the body.

DOI： 10.1016/j.celrep.2014.10.006

Critical importance of the surface expression of alpha 2,6-sialosides during early development of medaka fish

Kitajima Ken, Wu Di, Tajima Katsue, Maruyama Emi, Yasukawa Yuko, Chang Lan-Yi, Taniguchi Yoshihito, Kamei Yasuhiro, Adachi Tomoko, Hashimoto Hisashi, Hibi Masahiko, Fujita Akiko, Sato Chihiro

GLYCOBIOLOGY 24 巻 ( 11 ) 頁： 1110-1110 2014年11月

Interaction of 70-kDa heat shock protein with glycosaminoglycans and acidic glycopolymers. 査読有り

Harada Y, Garenáux E, Nagatsuka T, Uzawa H, Nishida Y, Sato C, Kitajima K

Biochemical and biophysical research communications 453 巻 ( 2 ) 頁： 229 - 34 2014年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Biochemical and Biophysical Research Communications

Interaction of Hsp70 with natural and artificial acidic glycans is demonstrated based on the native PAGE analysis. Hsp70 interacts with acidic glycopolymers that contain clustered sulfated and di-sialylated glycan moieties on a polyacrylamide backbone, but not with neutral or mono-sialylated glycopolymers. Hsp70 also interacts and forms a large complex with heparin, heparan sulfate, and dermatan sulfate that commonly contain 2-O-sulfated iduronic acid residues, but not with other types of glycosaminoglycans (GAGs). Hsp70 consists of the N-terminal ATPase domain and the C-terminal peptide-binding domain. The interaction analyses using the recombinant N- and C-terminal half domains show that the ATPase domain mediates the direct interaction with acidic glycans, while the peptide-binding domain stabilizes the large complexes with particular GAGs. To our knowledge, this is the first demonstration of direct binding of Hsp70 to the particular GAGs. This property may be involved in the physiological functions of Hsp70 at the plasma membrane and extracellular environments.

DOI： 10.1016/j.bbrc.2014.05.137

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PubMed

ST8 alpha-N-acetyl-neuraminide alpha- 2,8-sialyltransferase 2 (ST8SIA2)

Sato C.

Handbook of Glycosyltransferases and Related Genes, Second Edition 2 巻頁： 781-795 2014年1月

Polysialic acid: Biosynthesis, novel functions and applications. 査読有り

Colley K., Kitajima K, Sato C.

Critical Reviews in Biochemistry and Molecular Biology 頁： 1-35 2014年

Tissue-specific post-translational modification allows functional targeting of thyrotropin. 査読有り

Ikegami K, Liao X-H, Hoshino Y, Ono H, Ota W, Ito Y, Nishiwaki-Ohkawa T, Sato C, Kitajima K, Iigo M, Shigeyoshi Y, Yamada M, Murata Y, Refetoff S, Yoshimura T.

Cell Reports 9 巻頁： 1-9 2014年

Comparison of analytical methods to detect polysialic acid. 査読有り

Nishimura S, Hane M, Niimi Y, Miyata S, Kitajima K, and Sato C.

Journal of Glycomics and Lipidomics 4 巻頁： 113 2014年

Interaction of 70-kDa heat shock protein with glycosaminoglycans and acidc glycopolymers. 査読有り

Harada Y, Garenaux E, Nagatsuka T, Uzawa H, Nishida Y, Sato C, and Kitajima K.

Biochem Biophys Res Commun. 453 巻頁： 229-234 2014年

Frontal affinity chromatography: practice of weak interaction analysis between lectins and fluorescently labeled oligosaccharides. 査読有り

Sato C

Methods in molecular biology (Clifton, N.J.) 1200 巻頁： 257 - 64 2014年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Methods in Molecular Biology

Frontal affinity chromatography (FAC) is a simple and effective method that is applicable to the analysis of interactions between glycans and glycan-recognition proteins, including lectins, with weak affinity ranging from 10<sup>-4</sup> to 10<sup>-6</sup> (M) in terms of dissociation constant (K <inf>d</inf>). Using conventional instruments, such as a high-performance liquid chromatography (HPLC) system equipped with pump, injector, (fluorescent) detector, and data recorder, the dissociation constants for weak glycan-based interactions can be easily determined with high throughput and accuracy. Notably, if the glycans are labeled with fluorescent dyes, only a small amount of glycans is required for the analysis. Fluorescent labeling of glycans is a common technique, and an increasing number of fluorescent-labeled glycans are commercially available. In this chapter, an advanced FAC method using fluorescent-labeled glycans is described. © 2014 Springer Science+Business Media New York.

DOI： 10.1007/978-1-4939-1292-6_22

Web of Science

Scopus

PubMed

Comparison of analytical methods to detect polysialic acid. 査読有り

Nishimura S, Hane M, Niimi Y, Miyata S, Kitajima K, Sato C

Journal of Glycomics and Lipidomics 4 巻頁： 113 2014年

Polysialic acid: Biosynthesis, novel functions and applications. 査読有り

Colley K, Kitajima K, Sato C

Critical Reviews in Biochemistry and Molecular Biology 頁： 1-35 2014年

NMR study into the mechanism of recognition of the degree of polymerization by oligo/polysialic acid antibodies. 査読有り

Hanashima S, Sato C, Tanaka H, Takahashi T, Kitajima K, Yamaguchi Y.

Bioorg. Med. Chem. 21 巻頁： 6069-76 2013年10月

NMR study into the mechanism of recognition of the degree of polymerization by oligo/polysialic acid antibodies 査読有り

Hanashima Shinya, Sato Chihiro, Tanaka Hiroshi, Takahashi Takashi, Kitajima Ken, Yamaguchi Yoshiki

BIOORGANIC & MEDICINAL CHEMISTRY 21 巻 ( 19 ) 頁： 6069 - 6076 2013年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Bioorganic and Medicinal Chemistry

Oligo/polysialic acids consisting of consecutive α(2,8)-linkages on gangliosides and glycoproteins play a role in cell adhesion and differentiation events in a manner that is dependent on the degree of polymerization (DP). Anti-oligo/polysialic acid antibodies often have DP-dependent antigenic specificity, and such unique antibodies are often used in biological studies for the detection and differentiation of oligo/polysialic acids. However, molecular mechanisms remain unclear. We here use NMR techniques to analyze the binding epitopes of the anti-oligo/polysialic acid monoclonal antibodies (mAb) A2B5 and 12E3. The mAb A2B5, which has a preference for trisialic acid, recognizes sialic acid residues at the non-reducing terminus and those in nascent units. On the other hand, mAb 12E3, which prefers oligo/polysialic acids of more than six sugar units, recognizes inner sialic acid residues. In both structural complexes, the interresidue transferred NOE correlations are significantly different from those arising from analogs of the free states, indicating that the bound and free sugar conformations are distinct. The ability of the two mAbs to distinguish the chain lengths comes from different binding epitopes and possibly from the conformational differences in the oligo/polysialic acids. Information on the recognition modes is needed for the structural design of immunoreactive antigens for the development of high-affinity anti-polysialic acid antibodies and of related vaccines against pathogenic, polysialic acid-coated bacteria. © 2013 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.bmc.2013.07.023

Sato C.

Sialobiology: Structure, Biosynthesis and Function. Sialic Acid Glycoconjugates in Health and Disease 頁： 33-75 2013年8月

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