研究者詳細 - 佐藤　ちひろ

論文 - 佐藤　ちひろ

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Studies on chemical properties of cyclic sialic acid using their synthetic S- and O-glycosides as model compounds

Iwata, S; Sato, C; Ando, H; Kiso, M; Kannagi, R; Kitajima, K

GLYCOBIOLOGY 14 巻 ( 11 ) 頁： 1163 - 1163 2004年11月

Specificity of human trans-sialidase as probed with gangliosides

Nikonova, EY; Tertov, VV; Sato, C; Kitajima, K; Bovin, NV

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 14 巻 ( 20 ) 頁： 5161 - 5164 2004年10月

「シアル酸研究の新たな潮流」の特集にあたって Open Access

SATO Chihiro

Trends in glycoscience and glycotechnology 16 巻 ( 91 ) 頁： 291 - 292 2004年9月

Preface for the special issue entitled "new waves in sialobioscience" Open Access

Sato C.

Trends in Glycoscience and Glycotechnology 16 巻 ( 91 ) 頁： 291 - 292 2004年9月

Chain length diversity of sialic acids and its biological significance Open Access

Sato C.

Trends in Glycoscience and Glycotechnology 16 巻 ( 91 ) 頁： 331 - 344 2004年9月

記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：Trends in Glycoscience and Glycotechnology

Sialic acids are commonly present as monosialyl residues at the non-reducing terminal end of glycoconjugates. In some cases, αc2→8 linked di/oligosialic acid chains with DP 2 or 3 sialic acid residues are common structural units of gangliosides, and involved in various biological processes, such as cell adhesion, cell differentiation, signal transduction, and surface expression of stage specific antigen. In contrast, little attention has been paid to the occurrence and functions of such short sialyl di/oligomers on glycoproteins, while polysialic acid (DP≥8) in embryonic NCAM has been extensively studied as a regulator of cell adhesion in neurogenesis. As analytical methods to detect di/oligosialic acid structures have been improved, several glycoproteins containing di/oligo/polysialic acid chains have been identified. It is thus hypothesized that these di/oligosialic acid residues on glycoproteins may have similar important functions in common with those proposed for the gangliosides or may have new functions. Currently, several studies showing the importance of di/oligosialic acid-containing glycoproteins have emerged. In this review, recent advances in such studies of di/ oligosialic acid residues on glycoproteins, including analytical methods, occurrence, biosynthetic pathways, and functions, are described.

DOI： 10.4052/tigg.16.331

Open Access

Scopus

CiNii Research

Developmental Expression of a Sialyltransferase Responsible for Sialylation of Cortical Alveolus Glycoprotein during Oogenesis in Rainbow Trout (Oncorhynchus mykiss) Open Access

ASAHINA Shinji, SATO Chihiro, KITAJIMA Ken

Journal of Biochemistry 136 巻 ( 2 ) 頁： 189 - 198 2004年8月

Developmental expression of a sialyltransferase responsible for sialylation of cortical alveolus glycoprotein during oogenesis in rainbow trout (Oncorhynchus mykiss).

Asahina S, Sato C, Kitajima K

Journal of biochemistry 136 巻 ( 2 ) 頁： 189 - 98 2004年8月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of Biochemistry

Polysialoglycoprotein (PSGP) is a major cortical alveolus glycoprotein of rainbow trout eggs that is characterized by the attachment of a polysialic acid structure to its O-glycan chains. It has been demonstrated that the polysialic acid structure is synthesized by at least three sialyltransferases mostly localized in cortical alveoli. Here we have cloned a cDNA encoding the sialyltransferase, designated rtST6GalNAc, responsible for the transfer of the first sialic acid residue onto the O-glycan chain of PSGP. This enzyme belongs to the vertebrate ST6GalNAc II family, and is strongly expressed in ovaries. Of those O-glycoproteins tested as substrates, asialo-PSGP is the best substrate. These results indicate that rtST6GalNAc is the enzyme responsible for the sialylation of PSGP during oogenesis. Furthermore, the rtST6GalNAc mRNA is expressed throughout oogenesis, is down-regulated at the late yolk vesicle stage (May), and then up-regulated during vitellogenesis (until August). This developmental profile is highly similar to that of STL2, a cortical alveolus lectin, while it is quite different from that of PSGP, which is extensively expressed at the yolk vesicle stage and down-regulated at later stages. Thus, not all cortical alveolus components are transcribed concomitantly. This is the first description of a developmental change in the transcription of a glycosyltransferase during oogenesis.

DOI： 10.1093/jb/mvh106

Alpha-C-mannosyltryptophan is not recognized by conventional mannose-binding lectins.

Nishikawa T, Kajii S, Sato C, Yasukawa Z, Kitajima K, Isobe M

Bioorganic & medicinal chemistry 12 巻 ( 9 ) 頁： 2343 - 8 2004年5月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Bioorganic and Medicinal Chemistry

α-C-Mannosyltryptophan (C-Man-Trp) is a novel, naturally occurring C-linked carbohydrate-protein linkage first found in 1994 from human ribonuclease 2. Since then, a number of C-Man-Trp residue have been found from several important proteins such as interleukin 12β, components of complement system, thrombospondin-1, and erythropoietin receptor, however, the biological functions have remained unknown even though its biosynthetic pathway has been revealed. In order to find a clue as to the biological functions, we examined the affinity of C-Man-Trp with conventional mannose lectin such as concanavarin A (Con A) and mannose-binding lectin (MBL). The affinity of C-Man-Trp with Con A, a typical mannose-binding lectin from plant was examined using a Con A-Sepharose column. Unlike p-nitrophenyl-α-O-Man, C-Man-Trp was not retained on the column. MBL-C, a major mannose-binding lectin purified from mouse serum, did not bind with N-biotinylated C-Man-Trp, judging from ELISA based assay. These results imply that C-Man-Trp may be recognized with the other specific proteins associated with its unknown biological functions. © 2004 Elsevier Ltd.

DOI： 10.1016/j.bmc.2004.02.009

Web of Science

Scopus

PubMed

ジシアル酸・オリゴシアル酸・ポリシアル酸の生物学的機能

佐藤ちひろ

Functional Glycomics 3 頁： 40 - 47 2004年

FORMATION OF MEMBRANE MICRODOMAINS IS SIGNIFICANT IN DEVELOPING EMBRYOS OF MEDAKA(Developmental Biology,Abstracts of papers presented at the 74^<th> Annual Meeting of the Zoological Society of Japan) : Open Access

AdachiTomoko, SatoChihiro, KitajimaKen

Zoological science 20 巻 ( 12 ) 頁： 1570 2003年12月

[Frequent occurrence and biological significance of degree of polymerization in sialic acid]. 査読有り

Sato C

Seikagaku. The Journal of Japanese Biochemical Society 75 巻 ( 12 ) 頁： 1526 - 30 2003年12月

Frequent occurrence and biological significance of degree of polymerization in sialic acid

Sato C.

Seikagaku the Journal of Japanese Biochemical Society 75 巻 ( 12 ) 頁： 1526 - 1530 2003年12月

Discovery of two new polysialoglycoproteins in human and sea urchin. Linkage and length diversities of polysialic acid in animal glycoproteins

Kitajima, K; Yabe, U; Miyata, S; Sato, CH

GLYCOBIOLOGY 13 巻 ( 11 ) 頁： 844 - 844 2003年11月

FUNCTIONS OF THE COMMON GLYCOTOPES OF GLYCOLIPIDS AND GLYCOPROTEINS IN THE MEMBRANE MICRODOMAIN OF SEA URCHIN SPERM(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan) : Open Access

MiyataShinji, MaehashiEri, OhtaKaoru, SatoChihiro, MatsudaTsukasa, KitajimaKen

Zoological science 19 巻 ( 12 ) 頁： 1443 2002年12月

Neuronal differentiation-dependent expression of the disialic acid epitope on CD166 and its involvement in neurite formation in Neuro2A cells. Open Access

Sato C, Matsuda T, Kitajima K

The Journal of biological chemistry 277 巻 ( 47 ) 頁： 45299 - 305 2002年11月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of Biological Chemistry

We previously demonstrated that α2,8-linked disialic acid (diSia) residues occur in several glycoproteins of mammalian brains (Sato, C., Fukuoka, H., Ohta, K., Matsuda, T., Koshino, R., Kobayashi, K., Troy, F. A., II, and Kitajima, K. (2000) J. Biol. Chem. 275, 15422-15431). The role of the diSia epitope on these glycoproteins is not known, whereas the importance of the diSia epitope on glycolipids is well documented in neurite formation. In this study, we demonstrated that the diSia epitope (Neu5Acα2 → 8Neu5Acα2 → 3Gal) on glycoproteins, but not on glycolipids, is involved in neurite formation in a mouse neuroblastoma cell line, Neuro2A, based on the following lines of evidence. First, the amount of diSia epitope on glycoproteins increased during retinoic acid-induced neurite formation. Second, retinoic acid treatment primarily increased the diSia epitope on a 100-kDa glycoprotein. We identified this protein as CD166 (SC1), an immunoglobulin superfamily cell adhesion molecule involved in neurite extension. Third, a monoclonal antibody against the diSia epitope specifically inhibited neurite formation. We also demonstrated that α2,8-sialyltransferase III mRNA expression increased 1.7-fold after the induction of neurite formation, suggesting that α2,8-sialyltransferase III is responsible for formation of the diSia epitope on CD166.

DOI： 10.1074/jbc.M206046200

佐藤ちひろ, 北島健

6 巻頁： A4J 2002年6月

Function of Di/oligosialic Acid Structure

Sato Chihiro, Kitajima Ken

Glycoforum 6 巻頁： A4 2002年6月

Occurrence of Oligosialic Acids on Integrin α<inf>5</inf> Subunit and Their Involvement in Cell Adhesion to Fibronectin Open Access

Nadanaka S., Sato C., Kitajima K., Katagiri K., Irie S., Yamagata T.

Journal of Biological Chemistry 276 巻 ( 36 ) 頁： 33657 - 33664 2001年9月

記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of Biological Chemistry

Integrin α5β1, a major fibronectin receptor, functions in a wide variety of biological phenomena. We have found that α2-8-linked oligosialic acids with 5 ≤ degree of polymerization (DP) ≤ 7 occur on integrin α5 subunit of the human melanoma cell line G361. The integrin α5 subunit immunoprecipitated with anti-integrin α5 antibody reacted with the monoclonal antibody 12E3, which recognizes oligo/polysialic acid with DP ≥ 5 but not with the polyclonal antibody H.46 recognizing oligo/polysialic acid with DP ≥ 8. The occurrence of oligosialic acids was further demonstrated by fluorometric C7/C9 analysis on the immunopurified integrin α5 subunit. Oligosialic acids were also found in the α5 subunit of several other human cells such as foreskin fibroblast and chronic erythroleukemia K562 cells. These results suggest the ubiquitous modification with unique oligosialic acids occurs on the α 5 subunit of integrin α5β1. The adhesion of human melanoma G361 cells to fibronectin was mainly mediated by integrin α5β1. Treatment of cells with sialidase from Arthrobacter ureafaciens cleaving α2-3-, α2-6-, and α2-8-linked sialic acids inhibited adhesion to fibronectin. On the other hand, N-acetylneuraminidase II, which cleaves α2-3 and α2-6 but not α2-8 linkages, showed no inhibitory activity. After the loss of oligosialic acids, integrin α5β1 failed to bind to fibronectin-conjugated Sepharose, indicating that the oligosialic acid on the α5 subunit of integrin α5β 1 plays important roles in cell adhesion to fibronectin.

DOI： 10.1074/jbc.M011100200

Open Access

Scopus

Identification and Adipocyte Differentiation-dependent Expression of the Unique Disialic Acid Residue in an Adipose Tissue-specific Glycoprotein, Adipo Q Open Access

Sato C., Yasukawa Z., Honda N., Matsuda T., Kitajima K.

Journal of Biological Chemistry 276 巻 ( 31 ) 頁： 28849 - 28856 2001年8月

記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of Biological Chemistry

Recently, we have shown that α2,8-linked disialic acid (diSia) residue occurs in glycoproteins more frequently than ever recognized (Sato, C., Fukuoka, H., Ohta, K., Matsuda, T., Koshino, R., Kobayashi K., Troy, F. A., II, and Kitajima, K. (2000) J. Biol. Chem. 275, 15422-15431). In the course of identification of the diSia-containing glycoproteins in mammals, the 30-kDa glycoprotein was found in bovine serum. The 30-kDa glycoprotein was shown to be the bovine adipo Q, an adipocyte-specific protein, based on the partial amino acid sequences and the immuno-cross-reactivity with the recombinant mouse adipo Q. The bovine adipo Q was shown to have no N-linked but O-linked glycan(s) containing the diSia epitope, Neu5Acα2→8Neu5Acα2→3Gal. Furthermore, the diSia epitope was also found in the mouse adipo Q in serum as well as in the 3T3-L1 cells that are fully differentiated into adipocytes. Notably, among the known α2,8-sialyltransferases, only the α2,8-sialyltransferase III mRNA was detected in the 3T3-L1 cells at any stages of differentiation, and the recombinant α2,8-sialyltransferase III could sialylate the purified bovine adipo Q. Thus, this study clearly provides the new findings that adipo Q is the diSia-containing glycoprotein and a physiological substrate of α2,8-sialyltransferase III, whose substrates have not been identified so far.

DOI： 10.1074/jbc.M104148200

Open Access

Scopus

昆虫におけるシアル酸，その存在と意義 Open Access

佐藤ちひろ

Ｔｒｅｎｄｓ　ｉｎ　Ｇｌｙｃｏｓｃｉｅｎｃｅ　ａｎｄ　Ｇｌｙｃｏｔｅｃｈｎｏｌｏｇｙ 13 巻 ( 73 ) 頁： 507 - 517 2001年

記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：FCCA(Forum: Carbohydrates Coming of Age)

数種の昆虫の発生途上にシアル酸、主に、N-アセチルノイラミン酸が見出されてきている。我々はレクチンや抗体、単糖の様々な精製および分析法を用いることによって、ショウジョウバエ (Drosophila melanogaster) の神経発達領域およびセミ(Philaenus spumarius) のマルピーギ管にポリシアル酸を検出した。これらの昆虫の成体にシアル酸は検出されなかった。シアル酸の発現は in vivo では明らかであるが、多くの昆虫培養細胞系ではその存在が明らかにされていない。また、バキュロウイルスの発現系を用いて哺乳動物型の組換え糖タンパク質 (複合型シアリル化N-型糖鎖をもつもの)を生産することは利点が多くあるため、培養細胞におけるシアル酸の生合成機構の解明が生物工学的な観点から重要視されてきている。それらの研究によって明らかになったことは、糖鎖の改変は昆虫細胞に糖転移酵素群を発現させることによってのみ可能であることである。すなわちシアル酸の生合成に関わる酵素をコードするいくつかの外来遺伝子を導入しなくてはならない。さらに、昆虫細胞に関する研究によって、シアル酸は動物界の新口動物に限られているのではなく、昆虫のような原口動物にも存在することが明らかになった。このことはシアル酸の進化に対して新しい見解を投ずるものである。

DOI： 10.4052/tigg.13.507

CiNii Research

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