研究者詳細 - 佐藤　ちひろ

論文 - 佐藤　ちひろ

分割表示 401 件中 221 - 240 件目／全件表示 >>

Tissue-specific post-translational modification allows functional targeting of thyrotropin. 査読有り

Ikegami K, Liao X-H, Hoshino Y, Ono H, Ota W, Ito Y, Nishiwaki-Ohkawa T, Sato C, Kitajima K, Iigo M, Shigeyoshi Y, Yamada M, Murata Y, Refetoff S, Yoshimura T.

Cell Reports 9 巻頁： 1-9 2014年

Comparison of analytical methods to detect polysialic acid. 査読有り

Nishimura S, Hane M, Niimi Y, Miyata S, Kitajima K, and Sato C.

Journal of Glycomics and Lipidomics 4 巻頁： 113 2014年

Interaction of 70-kDa heat shock protein with glycosaminoglycans and acidc glycopolymers. 査読有り

Harada Y, Garenaux E, Nagatsuka T, Uzawa H, Nishida Y, Sato C, and Kitajima K.

Biochem Biophys Res Commun. 453 巻頁： 229-234 2014年

Frontal affinity chromatography: practice of weak interaction analysis between lectins and fluorescently labeled oligosaccharides. 査読有り

Sato C

Methods in molecular biology (Clifton, N.J.) 1200 巻頁： 257 - 64 2014年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Methods in Molecular Biology

Frontal affinity chromatography (FAC) is a simple and effective method that is applicable to the analysis of interactions between glycans and glycan-recognition proteins, including lectins, with weak affinity ranging from 10-4 to 10-6 (M) in terms of dissociation constant (K <inf>d</inf>). Using conventional instruments, such as a high-performance liquid chromatography (HPLC) system equipped with pump, injector, (fluorescent) detector, and data recorder, the dissociation constants for weak glycan-based interactions can be easily determined with high throughput and accuracy. Notably, if the glycans are labeled with fluorescent dyes, only a small amount of glycans is required for the analysis. Fluorescent labeling of glycans is a common technique, and an increasing number of fluorescent-labeled glycans are commercially available. In this chapter, an advanced FAC method using fluorescent-labeled glycans is described. © 2014 Springer Science+Business Media New York.

DOI： 10.1007/978-1-4939-1292-6_22

Web of Science

Scopus

PubMed

Comparison of analytical methods to detect polysialic acid. 査読有り

Nishimura S, Hane M, Niimi Y, Miyata S, Kitajima K, Sato C

Journal of Glycomics and Lipidomics 4 巻頁： 113 2014年

Polysialic acid: Biosynthesis, novel functions and applications. 査読有り

Colley K, Kitajima K, Sato C

Critical Reviews in Biochemistry and Molecular Biology 頁： 1-35 2014年

NMR study into the mechanism of recognition of the degree of polymerization by oligo/polysialic acid antibodies. 査読有り

Hanashima S, Sato C, Tanaka H, Takahashi T, Kitajima K, Yamaguchi Y.

Bioorg. Med. Chem. 21 巻頁： 6069-76 2013年10月

NMR study into the mechanism of recognition of the degree of polymerization by oligo/polysialic acid antibodies 査読有り

Hanashima, S; Sato, C; Tanaka, H; Takahashi, T; Kitajima, K; Yamaguchi, Y

BIOORGANIC & MEDICINAL CHEMISTRY 21 巻 ( 19 ) 頁： 6069 - 6076 2013年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Bioorganic and Medicinal Chemistry

Oligo/polysialic acids consisting of consecutive α(2,8)-linkages on gangliosides and glycoproteins play a role in cell adhesion and differentiation events in a manner that is dependent on the degree of polymerization (DP). Anti-oligo/polysialic acid antibodies often have DP-dependent antigenic specificity, and such unique antibodies are often used in biological studies for the detection and differentiation of oligo/polysialic acids. However, molecular mechanisms remain unclear. We here use NMR techniques to analyze the binding epitopes of the anti-oligo/polysialic acid monoclonal antibodies (mAb) A2B5 and 12E3. The mAb A2B5, which has a preference for trisialic acid, recognizes sialic acid residues at the non-reducing terminus and those in nascent units. On the other hand, mAb 12E3, which prefers oligo/polysialic acids of more than six sugar units, recognizes inner sialic acid residues. In both structural complexes, the interresidue transferred NOE correlations are significantly different from those arising from analogs of the free states, indicating that the bound and free sugar conformations are distinct. The ability of the two mAbs to distinguish the chain lengths comes from different binding epitopes and possibly from the conformational differences in the oligo/polysialic acids. Information on the recognition modes is needed for the structural design of immunoreactive antigens for the development of high-affinity anti-polysialic acid antibodies and of related vaccines against pathogenic, polysialic acid-coated bacteria. © 2013 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.bmc.2013.07.023

Sato C.

Sialobiology: Structure, Biosynthesis and Function. Sialic Acid Glycoconjugates in Health and Disease 頁： 33-75 2013年8月

Disialic, oligosialic and polysialic acids: distribution, functions and related disease. 査読有り

Sato C, Kitajima K

Journal of biochemistry 154 巻 ( 2 ) 頁： 115 - 36 2013年8月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of Biochemistry

Sialic acids (Sia) are involved in many biological activities and frequently exist as monosialyl residues at the non-reducing terminal end of glycoconjugates. Occasionally, polymerized structures in the form of disialic acid (diSia), oligosialic acid (oligoSia) and polysialic acid (polySia) are also found in glycoconjugates. In particular, polySia, which is an evolutionarily conserved epitope from sea urchin to humans, is one of the most biologically important glycotopes in vertebrates. The biological functions of polySia, especially on neural cell adhesion molecules, have been well studied and an in-depth body of knowledge concerning polySia has been accumulated. However, considerably less attention has been paid to glycoproteins containing di-and oligoSia groups. However, advances in analytical methods for detecting oligo/polymerized structures have allowed the identification and characterization of an increasing number of glycoproteins containing di/oligo/polySia chains in nature. In addition, sophisticated genetic techniques have also helped to elucidate the underlying mechanisms of polySia-mediated activities. In this review, recent advances in the study of the chemical properties, distribution and functions of di-, oligo-and polySia residues on glycoproteins are described. © 2013 The Authors.

DOI： 10.1093/jb/mvt057

Web of Science

Scopus

PubMed

Occurrence of free deaminoneuraminic acid (KDN)-containing complex-type N-glycans in human prostate cancers. 査読有り

Yabu M, Korekane H, Takahashi H, Ohigashi H, Ishikawa O, Hatano K, Nonomura N, Sato C, Kitajima K, Miyamoto Y.

Glycobiology 23 巻頁： 634-642 2013年

ポリシアル酸による神経機能の調節. 招待有り査読有り

佐藤ちひろ、北島健

実験医学 31 巻頁： 26--33 2013年

Disialic, oligosialic, and polysialic acids: Distribution, biological functions, and related disease. 招待有り査読有り

Sato C., Kitajima K.

J Biochem (Tokyo). 154 巻頁： 115-136 2013年

Impact of structural aberrancy of polysialic acid and its synthetic enzyme ST8SIA2 in schizophrenia. 査読有り Open Access

Sato C, Kitajima K.

Front. Cell. Neurosci. 7 巻 ( 61 ) 頁： 1-11 2013年

Crystal structure of anti-polysialic acid antibody single chain Fv fragment complexed with octasialic acid: insight into the binding preference for polysialic acid. 査読有り Open Access

Nagae M, Ikeda A, Hane M, Hanashima S, Kitajima K, Sato C, Yamaguchi Y.

J Biol Chem. 288 巻頁： 33784-96 2013年

ポリシアル酸による神経機能の調節. 招待有り査読有り

佐藤ちひろ, 北島健

実験医学 31 巻頁： 26--33 2013年

Crystal structure of anti-polysialic acid antibody single chain Fv fragment complexed with octasialic acid: insight into the binding preference for polysialic acid. 査読有り Open Access

Nagae M, Ikeda A, Hane M, Hanashima S, Kitajima K, Sato C, Yamaguchi Y

J Biol Chem. 288 巻頁： 33784-96 2013年

Impact of structural aberrancy of polysialic acid and its synthetic enzyme ST8SIA2 in schizophrenia. 査読有り Open Access

Sato C, Kitajima K

Front. Cell. Neurosci. 7 巻 ( 61 ) 頁： 1-11 2013年

Occurrence of free deaminoneuraminic acid (KDN)-containing complex-type N-glycans in human prostate cancers. 査読有り

Yabu M, Korekane H, Takahashi H, Ohigashi H, Ishikawa O, Hatano K, Nonomura N, Sato C, Kitajima K, Miyamoto Y

Glycobiology 23 巻頁： 634-642 2013年

Pig sperm membrane microdomains contain a highly glycosylated 15-25-kDa wheat germ agglutinin-binding protein. 査読有り

Kasekarn W, Kanazawa T, Hori K, Tsuchiyama T, Lian X, Garénaux E, Kongmanas K, Tanphaichitr N, Yasue H, Sato C, Kitajima K

Biochemical and biophysical research communications 426 巻 ( 3 ) 頁： 356 - 62 2012年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Biochemical and Biophysical Research Communications

A highly glycosylated protein, which has unique, novel features in localization, structure, and potential function, is found in pig sperm, and named WGA-gp due to its high binding property with wheat germ agglutinin (WGA). WGA-gp is localized mainly in flagella and enriched in membrane microdomains or lipid rafts. It is not detected by ordinary protein staining methods due to a high content of both N- and O-glycans consisting of neutral monosaccharides. Interestingly, WGA-gp may be involved in intracellular Ca2+ regulation. Treatment of sperm with anti-WGA-gp antibody enhances the amplitude of Ca2+ oscillation without changing the basal intracellular Ca2+ concentrations. All these features of WGA-gp, except for different carbohydrate structures occupying most part of the molecules, are similar to those of flagellasialin in sea urchin sperm, which regulates the intracellular Ca2+ concentration. Presence of carbohydrate-enriched flagellar proteins involved in intracellular Ca2+ regulation may be a common feature among animal sperm. © 2012 Elsevier Inc.

DOI： 10.1016/j.bbrc.2012.08.090

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論文 - 佐藤 ちひろ

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論文 - 佐藤　ちひろ