研究者詳細 - 佐藤　ちひろ

論文 - 佐藤　ちひろ

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Crystallization and preliminary X-ray analysis of mitochondrial presequence receptor Tom20 in complexes with a presequence from aldehyde dehydrogenase Open Access

Igura M, Ose T, Obita T, Sato C, Maenaka K, Endo T, Kohda D

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 61 巻 ( 5 ) 頁： 514-517 2005年5月

Distal myopathy with rimmed vacuoles - Impaired O-glycan formation in muscular glycoproteins Open Access

Tajima Y, Uyama E, Go S, Sato C, Tao N, Kotani M, Hino H, Suzuki A, Sanai Y, Kitajima K, Sakuraba H

AMERICAN JOURNAL OF PATHOLOGY 166 巻 ( 4 ) 頁： 1121-1130 2005年4月

Distal myopathy with rimmed vacuoles: impaired O-glycan formation in muscular glycoproteins. 査読有り Open Access

Tajima Y, Uyama E, Go S, Sato C, Tao N, Kotani M, Hino H, Suzuki A, Sanai Y, Kitajima K, Sakuraba H

The American journal of pathology 166 巻 ( 4 ) 頁： 1121 - 30 2005年4月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：American Journal of Pathology

Distal myopathy with rimmed vacuoles (DMRV), is an autosomal recessive disorder with early adult onset, displays distal dominant muscular involvement and is characterized by the presence of numerous rimmed vacuoles in the affected muscle fibers. The pathophysiology of DMRV has not been clarified yet, although the responsible gene was identified as that encoding UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase involved hi the biosynthesis of sialic acids. To identify defective carbohydrate moieties of muscular glycoproteins from DMRV patients, frozen skeletal muscle sections from seven patients with DMRV, as well as normal and pathological controls, were treated with or without sialidase or N-glycosidase F followed by lectin staining and lectin blotting analysis. The sialic acid contents of the O-glycans in the skeletal muscle specimens from the DMRV patients were also measured. We found that Arachis hypogaea agglutinin (PNA) lectin reacted strongly with sarcolemmal glycoproteins in the DMRV patients but not with those in control subjects. α-Dystroglycan from the DMRV patients strongly associated with PNA lectin, although that from controls did not. The sialic acid level of the O-glycans in the DMRV muscular glycoproteins with molecular weights of 30 to 200 kd was reduced to 60 to 80% of the control level. The results show that impaired sialyl O-glycan formation in muscular glycoproteins, including α-dystroglycan, occurs in DMRV.

DOI： 10.1016/S0002-9440(10)62332-2

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Differentiation of noncancerous tissue and cancer lesions by apparent diffusion coefficient values in transition and peripheral zones of the prostate Open Access

Sato C, Naganawa S, Nakamura T, Kumada H, Miura S, Takizawa O, Ishigaki T

JOURNAL OF MAGNETIC RESONANCE IMAGING 21 巻 ( 3 ) 頁： 258-262 2005年3月

Development of a simple and efficient method for assaying cytidine monophosphate sialic acid synthetase activity using an enzymatic reduced nicotinamide adenine dinucleotide/oxidized nicotinamide adenine dinucleotide converting system. 査読有り

Fujita A, Sato C, Münster-Kühnel AK, Gerardy-Schahn R, Kitajima K

Analytical biochemistry 337 巻 ( 1 ) 頁： 12 - 21 2005年2月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Analytical Biochemistry

A new reliable method to assay the activity of cytidine monophosphate sialic acid (CMP-Sia) synthetase (CSS) has been developed. The activation of sialic acids (Sia) to CMP-Sia is a prerequisite for the de novo synthesis of sialoglycoconjugates. In vertebrates, CSS has been cloned from human, mouse, and rainbow trout, and the crystal structure has been resolved for the mouse enzyme. The mouse and rainbow trout enzyme have been compared with respect to substrate specificity, demonstrating that the mouse enzyme exhibits a pronounced specificity for N-acetylneuraminic acid (Neu5Ac), while the rainbow trout CSS is equally active with either of three Sia species, Neu5Ac, N-glycolylneuraminic acid (Neu5Gc), and deaminoneuraminic acid (KDN). However, molecular details that explain the pronounced substrate specificities are unknown. Understanding the catalytic mechanisms of these enzymes is of major importance, since CSSs play crucial roles in cellular sialylation patterns and thus are potential drug targets in a number of pathophysiological situations. The availability of the cDNAs and the obtained structural data enable rational approaches; however, these efforts are limited by the lack of a reliable high-throughput assay system. Here we describe a new assay system that allows product quantification in a reduced nicotinamide adenine dinucleotide (NADH)-dependent color reaction. The activation reaction catalyzed by CSS, CTP + Sia → CMP-Sia + pyrophosphate, was evaluated by a consumption of Sia, which corresponds to that of NADH on the following two successive reactions: (i) Sia → pyruvate + ManNAc (or Man), catalyzed by a sialic acid lyase (SAL), and (ii) pyruvate + NADH → lactate + oxidized nicotinamide adenine dinucleotide (NAD <sup>+</sup>), catalyzed by a lactate dehydrogenase (LDH). Consumption of NADH can be photometrically monitored on a microtiter plate reader for a number of test samples at the same time. Furthermore, based on the quantification of CSS used in the SAL/LDH assay, relative activities toward Sia derivatives have been obtained. The preference of mouse CSS toward Neu5Ac and the ability of the rainbow trout enzyme to activate both KDN and Neu5Ac were confirmed. Thus, this simple and time-saving method is suitable for a systematic comparison of enzyme activity of structurally mutated enzymes based on the relative specific activity. © 2004 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.ab.2004.10.033

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Distal Myopathy with Rimmed Vaculoes. 査読有り Open Access

Tajima, Y., Uyama, E., Go, S., Sato, C., Tao, N., Kotani, M., Hino, H., Suzuki, A.,Sanai, Y., Kitajima, K., and Sakuraba, H.

Am. J. Pathol. 166 巻頁： 1121-1128 2005年

Inflammation-dependent changes in α2,3-, α2,6-, and α2,8-sialic acid glycotopes on serum glycoproteins in mouse. 査読有り Open Access

Yasukawa, Z., Sato, C., and Kitajima, K.

Glycobiology 15 巻頁： 827-837 2005年

A major flagellum sialoglycoprotein in sea urchin sperm contains a novel polysialic acid, an alpha2,9-linked poly-N-acetylneuraminic acid chain, capped by an 8-O-sulfated sialic acid residue. 査読有り Open Access

Miyata S, Sato C, Kitamura S, Toriyama M, Kitajima K

Glycobiology 14 巻 ( 9 ) 頁： 827 - 40 2004年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Glycobiology

A new type of polysialic acid (polySia) structure was demonstrated to occur in a major unknown sialoglycoprotein with a diverse molecular mass of 40-80 kDa in sea urchin sperm. The polySia-containing glycan structure was determined to be HSO<inf>3</inf>→8Neu5Acα2→9(Neu5Acα2→9 <inf>n-2</inf> Neu5Acα2→6GalNAcα1→Ser/Thr (n, on average 15), based on carbohydrate analysis of the sialoglycopeptide obtained by an exhaustive protease digestion of whole sperm, fluorometric anion-exchange high-performance liquid chromatography, and methylation analysis. The sulfate group was predominantly localized to the nonreducing terminus of the polySia chain. This is the first example of an α2,9-linked polySia structure in animal sperm. The polySia-containing sialoglycoprotein was present in sperm flagellum but not in the head. Furthermore, this sialoglycoprotein localized in the sperm lipid raft, which contains an enriched ganglioside (Neu5Acα 2→8Neu5Acα2→6GlcCer), a receptor for sperm-activating peptide (speract), and its associated guanylate cyclase. © Oxford University Press 2004; all rights reserved.

DOI： 10.1093/glycob/cwh100

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PubMed

Chain length diversity of sialic acids and its biological significance Open Access

Sato C

TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY 16 巻 ( 91 ) 頁： 331-344 2004年9月

母乳中に発見された新規ポリシアロ糖タンパク質 : CD36がユニークな糖鎖修飾を受けていることが明らかに

佐藤ちひろ

化学と生物 42 巻 ( 1 ) 頁： 12-14 2004年1月

alpha-C-Mannosyltryptophan is not recognized by conventional mannose-binding lectins 査読有り

T.Nishikawa, S. Kajii, C. Sato, Z. Yasukawa, K. Kitajima, and M. Isobe

Bioorg Med Chem. 頁： 2343-2348 2004年

A major flagellum sialoglycoprotein in sea urchin sperm contains a novel polysialic acid, an α2,9-linked poly-N-acetylneuraminic acid chain, capped by an 8-O-sulfated sialic acid residue 査読有り Open Access

S. Miyata, C. Sato, S. Kitamura, S. Toriyama, and K. Kitajima,

Glycobiology 14 巻頁： 827-840 2004年

記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1093/glycob/cwh100

Specificity of human trans-sialidase as probed with gangliosides. 査読有り

Nikonova, E. Y., Tertov, V. V., Sato, C., Kitajima, K., and Bovin, N. V.

Bioorg. Med. Chem. Lett. 14 巻頁： 5161-5164 2004年

A newly identified zona pellucida glycoprotein, ZPD and dimeric ZP1 of chicken egg envelope are involved in sperm activation on sperm-egg interaction 査読有り

H. Okumura, Y. Kohno, Y. Iwata, H. Mori, N. Aoki, C. Sato, K. Kitajima, D. Nadano, and T. Matsuda

Biochem. J. 383 巻頁： 1-9 2004年

Development of a simple and efficient method for assaying the CMP-sialic acid synthetase activity using an enzymatic NADH/NAD+ converting system 査読有り

A. Fujita, C. Sato, A.-K. Münster-Kühnel, R. Gerardy-Schahn, and K. Kitajima

Anal. Biochem. 337 巻頁： 12-21 2004年

*Chain length diversity of sialic acids and its biological significance (Review) 招待有り査読有り Open Access

C. Sato

Trends Glycosci. Glycotechnol 16 巻頁： 331-344 2004年

Developmental Expression of a Sialyltransferase Responsible for Sialylation of Cortical Alveolus Glycoprotein During Oogenesis in Rainbow Trout (Oncorhynchus mykiss) 査読有り

S. Asahina, C.Sato, and K. Kitajima

J. Biochem. 136 巻頁： 189-198 2004年

ジシアル酸・オリゴシアル酸・ポリシアル酸の生物学的機能招待有り

佐藤ちひろ

3 巻頁： 40-47 2004年

Specificity of human trans-sialidase as probed with gangliosides. 査読有り

Nikonova, E. Y, Tertov, V. V, Sato, C, Kitajima, K, Bovin, N. V

Bioorg. Med. Chem. Lett. 14 巻頁： 5161-5164 2004年

ジシアル酸・オリゴシアル酸・ポリシアル酸の生物学的機能招待有り

佐藤ちひろ

Functional Glycomics 3 巻頁： 40-47 2004年

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