研究者詳細 - 佐藤　ちひろ

論文 - 佐藤　ちひろ

分割表示 376 件中 321 - 340 件目／全件表示 >>

Discovery of two new polysialoglycoproteins in human and sea urchin. Linkage and length diversities of polysialic acid in animal glycoproteins

Kitajima K, Yabe U, Miyata S, Sato CH

GLYCOBIOLOGY 13 巻 ( 11 ) 頁： 844-844 2003年11月

Identification of the sea urchin 350-kDa sperm-binding protein as a new sialic acid-binding lectin that belongs to the heat shock protein 110 family: implication of its binding to gangliosides in sperm lipid rafts in fertilization. 査読有り

Maehashi E, Sato C, Ohta K, Harada Y, Matsuda T, Hirohashi N, Lennarz WJ, Kitajima K

The Journal of biological chemistry 278 巻 ( 43 ) 頁： 42050 - 7 2003年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of Biological Chemistry

The 350-kDa sperm-binding protein (SBP), a species-specific sperm-binding protein, is localized in the vitelline layer of sea urchin eggs. In this study, we have shown for the first time that sperm gangliosides are ligands for the intact glycosylated SBP. Using recombinant fragments of the SBP, the N-terminal heat shock protein 110-like domain was shown to be responsible for the binding. The intact SBP could bind various gangliosides, and the binding was sialidase-sensitive and inhibited by sialyllactose, thus indicating that it is the sialic acid-binding protein. Calcium and magnesium ions were not required but they did enhance the binding activity of SBP. The observation that bacterially expressed recombinant SBP and the sialidase-treated intact glycosylated SBP lost divalent cation-dependent enhancement of binding activity suggests that the sialylated carbohydrate moieties of the SBP may be involved in this property. Furthermore, the SBP was shown to bind sperm lipid rafts, in which gangliosides are enriched, and this binding was lost upon sialidase treatment of the lipid rafts. Finally, liposomes containing the ganglioside specifically inhibited fertilization. Taken together, these results allow us to identify SBP as a member of a new class of sialic acid-binding lectin belonging to the Hsp110 family, and indicate that SBP may be involved in interaction of sperm with the vitelline layer of the egg.

DOI： 10.1074/jbc.M307493200

Web of Science

Scopus

PubMed

[Oligosialic acid: a common carbohydrate epitope in glycoproteins and glycolipids]. 査読有り

Sato C, Kitajima K

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme 48 巻 ( 12 ) 頁： 1730 - 1736 2003年9月

Polysialic acid in human milk. CD36 is a new member of mammalian polysialic acid-containing glycoprotein. 査読有り Open Access

Yabe U, Sato C, Matsuda T, Kitajima K

The Journal of biological chemistry 278 巻 ( 16 ) 頁： 13875 - 80 2003年4月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of Biological Chemistry

The neural cell adhesion molecule and the voltage-sensitive sodium channel α-subunit are the only two molecules in mammals known to be modified by α-2,8-linked polysialic acid (polySia). We found a new polySia-containing glycoprotein in human milk and identified it as CD36, a member of the B class of the scavenger receptor superfamily. The polysia-containing glycan chain(s) were removed by alkaline treatment but not by peptide: N-glycanase F digestion, indicating that milk CD36 contained polysia on O-linked glycan chain(s). Polysialylation of CD36 occurs not only in human milk but also in mouse milk. However, CD36 in human platelets is not polysialylated. PolySia CD36 is secreted in milk at any lactation stage and reaches peak level at 1 month after parturition. Thus, it is suggested that polySia of milk CD36 is significant for neonatal development in terms of protection and nutrition.

DOI： 10.1074/jbc.M300458200

Polysialic acid in human milk 査読有り

U. Yabe, C. Sato, T. Matsuda, and K. Kitajima

J. Biol. Chem. 278 巻頁： 13875-13880 2003年

ポリシアル酸の鎖長多様性とタンパク質機能招待有り査読有り

佐藤ちひろ

生化学 75 巻頁： 1526-1530 2003年

糖タンパク質と糖脂質の共通糖鎖オリゴシアル酸の普遍的存在とその意義招待有り

佐藤ちひろ、北島　健

蛋白質・核酸・酵素 48 巻頁： 1730-1736 2003年

Identification of the sea urchin 350 kDa sperm binding protein as a new sialic acid-binding lectin that belongs to the heat shock protein 110 family. 査読有り

E. Maehashi, C. Sato, K. Ohta, Y. Harada, T., Matsuda, N. Hirohashi, W. J. Lennarz, and K. Kitajima

J. Biol. Chem. 278 巻頁： 42050-42057 2003年

ポリシアル酸の鎖長多様性とタンパク質機能招待有り査読有り

佐藤ちひろ

生化学 75 巻頁： 1526-1530 2003年

糖タンパク質と糖脂質の共通糖鎖オリゴシアル酸の普遍的存在とその意義招待有り

佐藤ちひろ, 北島健

蛋白質・核酸・酵素 48 巻頁： 1730-1736 2003年

マウス炎症時における血清糖タンパク質上のシアル酸構造の変動

安川然太, 佐藤ちひろ, 松田幹, 北島健

生化学 74 巻 ( 11 ) 頁： 1389 - 1389 2002年11月

炎症誘導マウスにおけるジ及びオリゴシアル酸含有血清糖タンパク質の解析

安川然太, 佐藤ちひろ, 北島健

生化学 74 巻 ( 8 ) 頁： 726 - 726 2002年8月

*Neuronal differentiation-dependent expression of the disialic acid epitope on CD166 and its involvement in neurite formation in Neuro2A cells. 査読有り

C. Sato, T. Matsuda, and K. Kitajima

J. Biol. Chem. 277 巻頁： 45299-45303 2002年

*Neuronal differentiation-dependent expression of the disialic acid epitope on CD166 and its involvement in neurite formation in Neuro2A cells. 査読有り

C. Sato, T. Matsuda, K. Kitajima

J. Biol. Chem. 277 巻頁： 45299-45303 2002年

Occurrence of oligosialic acids on integrin alpha(5) subunit and their involvement in cell adhesion to fibronectin

Nadanaka S, Sato C, Kitajima K, Katagiri K, Irie S, Yamagata T

JOURNAL OF BIOLOGICAL CHEMISTRY 276 巻 ( 36 ) 頁： 33657-33664 2001年9月

Identification and adipocyte differentiation-dependent expression of the unique disialic acid residue in an adipose tissue-specific glycoprotein, adipo Q

Sato C, Yasukawa Z, Honda N, Matsuda T, Kitajima K

JOURNAL OF BIOLOGICAL CHEMISTRY 276 巻 ( 31 ) 頁： 28849-28856 2001年8月

脂肪細胞特異的血清タンパク質adipo Qのジ/オリゴシアル酸含有糖鎖の構造

安川然太, 佐藤ちひろ, 本田直輝, 松田幹, 北島健

生化学 73 巻 ( 8 ) 頁： 695 - 695 2001年8月

Identification and adipocyte differentiation-dependent expression of the unique disialic acid residues in an adipose tissue specific glycoprotein, adipo Q 査読有り

"C. Sato, Z. Yasukawa, N. Honda, T. Matsuda, and K. Kitajima"

J. Biol. Chem. 276 巻頁： 28849-28856 2001年1月

Occurrence of oligosialic acids on integrin a5 subunit and their involvement in cell adhesion to fibronectin. 査読有り

"S. Nadanaka, C. Sato, K. Kitajima, K. Katagiri, S. Irie, and T. Yamagata 276 (2001) in press"

J. Biol. Chem. 276 巻頁： 33657-33664 2001年1月

Identification and adipocyte differentiation-dependent expression of the unique disialic acid residues in an adipose tissue specific glycoprotein, adipo Q 査読有り

C. Sato, Z. Yasukawa, N. Honda, T. Matsuda, K. Kitajima

J. Biol. Chem. 276 巻頁： 28849-28856 2001年1月

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