Faculty Profiles - MIZUNO, Masashi

Low Serum Cultured Adipose Tissue-Derived Stromal Cells Ameliorate Acute Kidney Injury in Rats Reviewed International journal

Katsuno Takayuki, Ozaki Takenori, Saka Yosuke, Furuhashi Kazuhiro, Kim Hangsoo, Yasuda Kaoru, Yamamoto Tokunori, Sato Waichi, Tsuboi Naotake, Mizuno Masashi, Ito Yasuhiko, Imai Enyu, Matsuo Seiichi, Maruyama Shoichi

CELL TRANSPLANTATION Vol. 22 ( 2 ) page： 287 - 297 2013

Perforative Peritonitis caused by Appendicitis in a Patient on Peritoneal Dialysis Reviewed International journal

Mizuno Masashi, Suzuki Yasuhiro, Nonaka Keisuke, Sei Yumi, Maruyama Shoichi, Matsuo Seiichi, Ito Yasuhiko

INTERNAL MEDICINE Vol. 52 ( 11 ) page： 1177 - 1181 2013

Language：English Publishing type：Research paper (scientific journal) Publisher：一般社団法人日本内科学会

A 46-year-old man on peritoneal dialysis (PD) was hospitalized due to suspicious PD-related peritonitis. Because the patient's abdominal pain was unimproved by conventional antibiotics and multiple bacteria were identified in a smear-sample of PD fluid, endogenous peritonitis was suspected. Perforated appendicitis was finally diagnosed under exploratory laparotomy. In this patient, perforated appendicitis was difficult to diagnose due to the attenuated clinical symptoms and inconclusive results of abdominal computed tomography (CT), even though the positive predictive value of CT is >95% in non-PD patients. Quickly deciding to perform exploratory laparotomy in patients suspected of having endogenous peritonitis is thus important, even when the origin has not been clarified.

DOI： 10.2169/internalmedicine.52.9196

Rat adipose tissue-derived stromal cells in a low serum medium attenuate peritoneal injuries in rat zymosan-induced peritonitis Reviewed

Hangsoo Kim, Masashi Mizuno, Kazuhiro Furuhashi, Takayuki Katsuno, Takenori Ozaki, Kaoru Yasuda, Naotake Tsuboi, Enyu Imai, Seiichi Matsuo, Yasuhiko Ito, Shoichi Maruyama

IMMUNOBIOLOGY Vol. 217 ( 11 ) page： 1197 - 1197 2012.11

Short-term Administration of Diclofenac Sodium Affects Renal Function After Laparoscopic Radical Nephrectomy in Elderly Patients Reviewed International journal

Mizuno Tomohiro, Ito Kazuma, Miyagawa Yasuhiro, Ishikawa Kazuhiro, Suzuki Yasuhiro, Mizuno Masashi, Ito Yasuhiko, Funahashi Yasuhito, Hattori Ryohei, Gotoh Momokazu, Yamada Kiyofumi, Noda Yukihiro

JAPANESE JOURNAL OF CLINICAL ONCOLOGY Vol. 42 ( 11 ) page： 1073 - 1078 2012.11

C5a is a target to prevent peritoneal tissue damage in acute peritoneal injury in rats

Tomohiro Mizuno, Masashi Mizuno, Masaki Imai, Yasuhiro Suzuki, Mayu Kushida, Kiyofumi Yamada, Yukihiro Noda, Shoichi Maruyama, Hidechika Okada, Noriko Okada, Seiichi Matsuo, Yasuhiko Ito

IMMUNOBIOLOGY Vol. 217 ( 11 ) page： 1144 - 1144 2012.11

A rare case of acute kidney injury associated with autoimmune hemolytic anemia and thrombocytopenia after long-term usage of oxaliplatin Reviewed International journal

ITO Isao, ITO Yasuhiko, MIZUNO Masashi, SUZUKI Yasuhiro, YASUDA Kaoru, OZAKI Takenori, KOSUGI Tomoki, YASUDA Yoshinari, SATO Waichi, TSUBOI Naotake, MARUYAMA Shoichi, IMAI Enyu, MATSUO Seiichi

Vol. 16 ( 3 ) page： 490 - 494 2012.6

A rare case of acute kidney injury associated with autoimmune hemolytic anemia and thrombocytopenia after long-term usage of oxaliplatin Reviewed International journal

Isao Ito, Yasuhiko Ito, Masashi Mizuno, Yasuhiro Suzuki, Kaoru Yasuda, Takenori Ozaki, Tomoki Kosugi, Yoshinari Yasuda, Waichi Sato, Naotake Tsuboi, Shoichi Maruyama, Enyu Imai, Seiichi Matsuo

CLINICAL AND EXPERIMENTAL NEPHROLOGY Vol. 16 ( 3 ) page： 490 - 494 2012.6

Language：English Publishing type：Research paper (scientific journal) Publisher：SPRINGER

Oxaliplatin is effective in advanced colorectal cancer and is known to have relatively few side effects, such as hemolysis and renal toxicity. We report a case of acute kidney injury (AKI) after treatment with a combination of oxaliplatin, folinic acid and 5-fluorouracil or capecitabine. The patient developed acute renal failure, hemolytic anemia and thrombocytopenia after the 34th course of chemotherapy including oxaliplatin. A positive direct antiglobulin test and detection of immunoglobulin G and complement C3b and C3d on erythrocytes suggested the diagnosis of immune-related severe intravascular hemolytic anemia. She was successfully treated and recovered using plasma exchange, corticosteroids and hemodialysis therapy. Only seven other cases of AKI associated with oxaliplatin use have been reported to date. As in this case, acute hemolysis due to autoimmune mechanisms and subsequent AKI occurred suddenly after frequent use of oxaliplatin in four of those cases. We should be aware that oxaliplatin may cause sudden life-threatening hemolysis by drug-induced antibodies and subsequent AKI, even though oxaliplatin is frequently administered without side effects. This represents the first case report of AKI-related hemolysis due to oxaliplatin in Japan.

DOI： 10.1007/s10157-012-0620-8

Asymptomatic diverticulosis identified by computed tomography is not a risk factor for enteric peritonitis International journal

Susumu Toda, Yasuhiko Ito, Masashi Mizuno, Yasuhiro Suzuki, Isao Ito, Hideki Hiramatsu, Takenori Ozaki, Naotake Tsuboi, Waichi Sato, Shoichi Maruyama, Enyu Imai, Seiichi Matsuo

NEPHROLOGY DIALYSIS TRANSPLANTATION Vol. 27 ( 6 ) page： 2511 - 2516 2012.6

Language：English Publishing type：Research paper (scientific journal) Publisher：OXFORD UNIV PRESS

Background. Colonic diverticulitis is an important cause of polymicrobial peritonitis, which requires surgical treatment and cessation of peritoneal dialysis (PD). The aim of this study was to examine whether plain abdominal computed tomography (CT) is useful for evaluating colonic diverticulosis in chronic kidney disease (CKD) patients and to explore whether colonic diverticulosis is a risk factor for enteric peritonitis.
Methods. The subjects consisted of 137 consecutive CKD patients (Stage 4 or 5) who were candidates for PD from February 2005 to November 2009. Abdominal CT without contrast media was performed in all PD candidates.
Results. Diverticula of the colon were detected by plain CT in 57 cases (41.6%). The number of diverticula tended to increase with age. The most common site of involvement of diverticulosis was the ascending colon. In patients treated with PD, the incidence of peritonitis was higher in patients with diverticulosis than in those without diverticulosis (P = 0.004). However, only one episode of enteric peritonitis was observed among patients with diverticulosis. The presence of diverticulosis did not affect cumulative or technical survival. PD was not selected in four cases due to a high frequency of diverticula with episodes of abdominal pain. Two cases developed severe diverticulitis with peritonitis and underwent resection of the colon.
Conclusions. Our study suggests that plain CT examination is useful for detecting diverticulosis in CKD patients. Silent diverticulosis is not a risk factor for enteric diverticulosis-related peritonitis. PD may be contraindicated in cases having frequent diverticulosis with episodes of lower abdominal pain.

DOI： 10.1093/ndt/gfr685

Transforming growth factor-beta induces vascular endothelial growth factor-C expression leading to lymphangiogenesis in rat unilateral ureteral obstruction International journal

Suzuki Yasuhiro, Ito Yasuhiko, Mizuno Masashi, Kinashi Hiroshi, Sawai Akiho, Noda Yukihiro, Mizuno Tomohiro, Shimizu Hideaki, Fujita Yoshiro, Matsui Katsuyuki, Maruyama Shoichi, Imai Enyu, Matsuo Seiichi, Takei Yoshifumi

KIDNEY INTERNATIONAL Vol. 81 ( 9 ) page： 865 - 879 2012.5

Atrial natriuretic peptide ameliorates peritoneal fibrosis in rat peritonitis model Reviewed International journal

Hiroshi Kato, Tomohiro Mizuno, Masashi Mizuno, Akiho Sawai, Yasuhiro Suzuki, Hiroshi Kinashi, Fumiko Nagura, Shoichi Maruyama, Yukihiro Noda, Kiyofumi Yamada, Seiichi Matsuo, Yasuhiko Ito

NEPHROLOGY DIALYSIS TRANSPLANTATION Vol. 27 ( 2 ) page： 526 - 536 2012.2

Language：English Publishing type：Research paper (scientific journal) Publisher：OXFORD UNIV PRESS

Atrial natriuretic peptide (ANP) was recently reported to ameliorate fibrosis in the heart and experimental renal diseases and vascular thickening after balloon injury. Peritoneal fibrosis is an important complication of long-term peritoneal dialysis, and peritonitis is a factor in its onset. In the present study, we investigated the effects of ANP in a rat peritonitis-induced peritoneal fibrosis model.
As pretreatment, an osmotic pump containing vehicle (saline) or ANP (0.15 or 0.3 mu g/min) was inserted through the carotid vein in male Sprague-Dawley rats. ANP or saline was continuously infused using the osmotic pump. Three days after administration of ANP or saline, rats underwent peritoneal scraping in a blind manner and were sacrificed on Day 14. The effects of ANP were evaluated based on peritoneal thickness, immunohistochemistry and real-time polymerase chain reaction. In each experiment, we evaluated messenger RNA (mRNA) expression of the ANP receptor natriuretic peptide receptor A (NPR-A) in the peritoneum after scraping. The effects of ANP were also studied in cultured peritoneal fibroblasts and mesothelial cells.
We observed a significant increase in NPR-A mRNA in the peritoneum. Peritoneal thickness increased with time and peaked on Day 14, but ANP significantly reduced peritoneal thickness. Parameters such as number of macrophages and CD-31-positive vessels and expression of type III collagen/transforming growth factor-beta/plasminogen activator inhibitor-1 (PAI-1)/connective tissue growth factor (CTGF) were significantly suppressed by ANP. In cultured peritoneal fibroblasts and mesothelial cells, ANP suppressed angiotensin II-induced upregulation of CTGF and PAI-1.
Our results suggest that ANP is useful in preventing inflammation-induced peritoneal fibrosis.

DOI： 10.1093/ndt/gfr302