Faculty Profiles - MIZUNO, Masashi

Papers - MIZUNO, Masashi

Division display 101 - 120 of about 350 ／ All the affair displays >>

Mouse NC/Jic strain provides novel insights into host genetic factors for malaria research Reviewed International journal Open Access

Miyasaka Yuki, Ohno Tamio, Kuga Masako, Ushida Kaori, Matsushima Miyoko, Kawabe Tsutomu, Takahashi Masahide, Mizuno Masashi, Kikkawa Yoshiaki

Experimental Animals Vol. 68 ( 3 ) page： 243 - 255 2019.7

Language：English Publishing type：Research paper (scientific journal) Publisher：Japanese Association for Laboratory Animal Science

Malaria is caused by Plasmodium parasites and is one of the most life-threatening infectious diseases in humans. Infection can result in severe complications such as cerebral malaria, acute lung injury/acute respiratory distress syndrome, and acute renal injury. These complications are mainly caused by P. falciparum infection and are major causes of death associated with malaria. There are a few species of rodent-infective malaria parasites, and mice infected with such parasites are now widely used for screening candidate drugs and vaccines and for studying host immune responses and pathogenesis associated with disease-related complications. We found that mice of the NC/Jic strain infected with rodent malarial parasites exhibit distinctive disease-related complications such as cerebral malaria and nephrotic syndrome, in addition to a rapid increase in parasitemia. Here, we focus on the analysis of host genetic factors that affect malarial pathogenesis and describe the characteristic features, utility, and future prospects for exploitation of the NC/Jic strain as a novel mouse model for malaria research.

DOI： 10.1538/expanim.18-0185

Effects of long-term treatment with low-GDP, pH-neutral solutions on peritoneal membranes in peritoneal dialysis patients. Reviewed International journal

Tawada M, Hamada C, Suzuki Y, Sakata F, Sun T, Kinashi H, Katsuno T, Takei Y, Maruyama S, Honda K, Mizuno M, Ito Y

Clinical and experimental nephrology Vol. 23 ( 5 ) page： 689 - 699 2019.5

Differences in peritoneal solute transport rates in peritoneal dialysis. Reviewed

Marina Asano, Takako Ishii, Akiyoshi Hirayama, Masashi Mizuno, Yasuhiro Suzuki, Fumiko Sakata, Shin-Ichi Akiyama, Shoichi Maruyama, Tomoyoshi Soga, Hiroshi Kinashi, Takayuki Katsuno, Yasuhiko Ito

Clinical and experimental nephrology Vol. 23 ( 1 ) page： 122 - 134 2019.1

Language：English Publishing type：Research paper (scientific journal)

BACKGROUND: Ultrafiltration failure associated with peritoneal membrane dysfunction is one of the main complications for patients on long-term peritoneal dialysis (PD). The dialysate-to-plasma concentration ratio (D/P) of creatinine is widely used to assess peritoneal membrane function. However, other small-sized solutes have not been studied in detail as potential indicators of peritoneal permeability. METHODS: We studied the D/Ps of small, middle-sized and large molecules in peritoneal equilibration tests in 50 PD patients. We applied metabolomic analysis of comprehensive small molecular metabolites using capillary electrophoresis time-of-flight mass spectrometry. RESULTS: D/Ps of middle-sized and large molecules correlated positively with D/P creatinine. Most D/Ps of small molecules correlated positively with D/P creatinine. Among 38 small molecules contained in the dialysate, urea, citrulline and choline showed significantly lower ability to permeate than creatinine. In the relationship between D/Ps of creatinine and small molecules, regression coefficients of three molecules were less than 0.3, representing no correlation to D/P creatinine. Five molecules showed negative regression coefficients. Among these molecules, hippurate and 3-indoxyl sulfate showed relatively high teinpro binding rates, which may affect permeability. Serum concentrations of two molecules were higher in the Low Kt/V group, mainly due to high protein binding rates. CONCLUSIONS: D/Ps of some molecules did not correlate with D/P creatinine. Factors other than molecular weight, such as charge and protein binding rate, are involved in peritoneal transport rates. Metabolomic analysis appears useful to analyze small molecular uremic toxins, which could accumulate in PD patients, and the status of peritoneal membrane transport for each molecule.

DOI： 10.1007/s10157-018-1611-1

Web of Science

PubMed

researchmap

Safety and effectiveness of eculizumab for adult patients with atypical hemolytic-uremic syndrome in Japan: interim analysis of post-marketing surveillance. Reviewed International coauthorship International journal Open Access

Hideki Kato, Yoshitaka Miyakawa, Yoshihiko Hidaka, Norimitsu Inoue, Shuichi Ito, Shoji Kagami, Shinya Kaname, Masanori Matsumoto, Masashi Mizuno, Takahisa Matsuda, Akihiko Shimono, Shoichi Maruyama, Yoshihiro Fujimura, Masaomi Nangaku, Hirokazu Okada

Clinical and experimental nephrology Vol. 23 ( 1 ) page： 65 - 75 2019.1

Language：English Publishing type：Research paper (scientific journal)

BACKGROUND: Eculizumab has been available for the treatment of atypical hemolytic-uremic syndrome (aHUS) in Japan since 2013. To assess safety and effectiveness of eculizumab in adult aHUS patients in the real-life setting, we performed interim analysis of a post-marketing surveillance mandated by Japanese regulations. METHODS: This study enrolled any patient who was diagnosed with TMA excluding Shiga toxin-producing Escherichia coli-HUS or thrombotic thrombocytopenic purpura based on Japanese clinical guide published in 2013 as inclusion criteria and treated with eculizumab. Although the term aHUS was redefined to denote only complement-mediated HUS in the guide revised in 2016, the patients with TMA caused by other causes (secondary TMA) were included. Patient outcomes and safety were evaluated at 6 months, 12 months, and annually thereafter. RESULTS: Thirty-three patients with aHUS and 27 patients with secondary TMA were enrolled. Median treatment duration of aHUS was 24weeks. Complement genes variants were detected in 11 of 18 patients with aHUS (61.1%). Among the 29 aHUS patients with available baseline data, platelet count (PLT), lactic dehydrogenase and serum creatinine (SCr) improved within 1-month after eculizumab initiation. TMA event-free status, complete TMA response, PLT normalization, and SCr decrease were achieved in 67.9% (19/28), 27.8% (5/18), 56.5% (13/23), and 57.1% (16/28) of patients, respectively. Thirty-three and 11 adverse reactions were observed in patients with aHUS (13/33 patients) and secondary TMA (6/27 patients), respectively. CONCLUSIONS: This interim analysis confirmed the acceptable safety profile and effectiveness of eculizumab for Japanese adult aHUS patients in real-world settings.

DOI： 10.1007/s10157-018-1609-8

HIV-associated Immune Complex Kidney Disease with C3-dominant Deposition Induced by HIV Infection after Treatment of IgA Nephropathy Reviewed International journal Open Access

Otaka Nozomu, Kawakita Chieko, Sugiyama Hitoshi, Kusano Nobuchika, Kinomura Masaru, Mizuno Masashi, Wada Jun, Kitagawa Masashi

Internal Medicine Vol. 58 ( 20 ) page： 3001 - 3007 2019

Language：English Publishing type：Research paper (scientific journal) Publisher：The Japanese Society of Internal Medicine

A 57-year-old man was diagnosed with IgA nephropathy. Hematuria and proteinuria were improved by tonsillectomy plus methylprednisolone pulse therapy. Lymphadenopathy, hypocomplementemia and pancytopenia were observed six years later, and urinalysis abnormalities recurred. A biopsy revealed mesangial proliferative glomerulonephritis with C3-dominant deposition. Human immunodeficiency virus (HIV) antibody demonstrated positive conversion. He was diagnosed with HIV-associated immune complex kidney disease (HIVICK). The hematuria, proteinuria and hypocomplementemia were improved by reducing the HIV viral load through antiretroviral therapy. When C3-dominant deposition is observed on a renal biopsy, HIVICK must be differentiated.

DOI： 10.2169/internalmedicine.2439-18

Investigation on the benefits of mycophenolate mofetil and therapeutic drug monitoring in the treatment of Japanese patients with lupus nephritis Reviewed International journal Open Access

Takayuki Katsuno, Takenori Ozaki, Takaya Ozeki, Asaka Hachiya, Hangsoo Kim, Noritoshi Kato, Takuji Ishimoto, Sawako Kato, Tomoki Kosugi, Naotake Tsuboi, Masashi Mizuno, Yasuhiko Ito, Shoichi Maruyama

Clinical and Experimental Nephrology Vol. 22 ( 6 ) page： 1341 - 1350 2018.12

Language：English Publishing type：Research paper (scientific journal) Publisher：Springer Tokyo

Background: Mycophenolate mofetil (MMF) is recommended as a first-line immunosuppressant to treat lupus nephritis (LN). Prognosis and therapeutic response in LN are known to vary depending on race. We investigated the benefits of MMF and therapeutic drug monitoring (TDM) in the treatment of Japanese LN patients. Methods: In this retrospective cohort study, a total of 20 patients with LN who started MMF treatment were included. Clinical data were collected regularly after MMF administration. We evaluated complete remission (CR) rate as the primary outcome. Predictors of CR were identified using univariate and multivariate analyses. In the research of TDM, the correlation with the area under the curve (AUC) was analyzed at MMF dose, single-point value, treatment response, and adverse events. Results: Overall, 70% of cases showed CR
both flare-ups and refractory cases had favorable results. Cases of LN with nephrotic syndrome (NS) or class III/IV + V showed a significantly lower CR rate (p &lt
0.005). The ratio of maintaining CR after MMF therapy was as high as 85.7%. In multivariate analysis, NS was an independent negative predictor of CR (HR 0.09, 95% confidence interval 0.01–0.81
p = 0.03). The relationship between AUC and MMF dose was low, and AUC correlated with trough level (r = 0.73). AUC tended to be high in the treatment responder (p = 0.09), but did not correlate with adverse events of infection (p = 0.92). Conclusion: MMF is a beneficial treatment option for Japanese LN patients, and further investigation on TDM-based therapy is needed.

DOI： 10.1007/s10157-018-1590-2

Extracellular histones decrease the expression of membrane complement regulators Reviewed International journal

Mizuno Tomohiro, Nagano Fumihiko, Mizuno Masashi, Iwata Ayumi, Takahashi Kazuo, Tsuboi Naotake, Maruyama Shoichi, Nagamatsu Tadashi, Imai Masaki

MOLECULAR IMMUNOLOGY Vol. 102 page： 189-189 2018.10

Peritoneal expression of membrane complement regulators in peritoneal dialysis patients with fungal peritonitis

Fukui Sosuke, Suzuki Yasuhiro, Tawada Mitsuhiro, Matsukawa Yoshihisa, Imai Masaki, Maruyama Shoichi, Ito Yasuhiko, Mizuno Masashi

MOLECULAR IMMUNOLOGY Vol. 102 page： 152 - 152 2018.10

Comprehensive analysis of complement proteins and genes in thrombotic microangiopathy in Japan

Hidaka, Y; Inoue, N; Ohtani, K; Ohtsuka, Y; Sawai, T; Miyata, T; Ohsawa, I; Okada, H; Kinoshita, T; Sekine, H; Tsukamoto, H; Nakao, M; Mizuno, M; Murakami, Y; Horiuchi, T; Wakamiya, N

MOLECULAR IMMUNOLOGY Vol. 102 page： 163 - 163 2018.10

SODIUM RESTRICTED DIET DURING CONSERVATIVE THERAPY IN PATIENTS WITH END-STAGE RENAL DISEASES (ESRD) MAY RESCUE RESIDUAL RENAL FUNCTION (RRF) FOR THE FIRST YEAR AFTER STARTING PERITONEAL DIALYSIS (PD)

Hiramatsu Tetsuaki, Mizuno Masashi, Nomori Sumiyo, Sakata Fumiko, Suzuki Yasuhiro, Maruyama Shoichi, Yasuhiko Ito, Noguchi Satoshi

NEPHROLOGY DIALYSIS TRANSPLANTATION Vol. 33 page： . 2018.5

高齢腹膜透析患者の地域連携における現状と課題

伊藤恭彦, 水野正司, 鈴木康弘, 杉山斉, 深水圭, 猪阪善隆

医工学治療 Vol. 30 ( Suppl. ) page： 161 - 161 2018.3

TGF-beta 1-VEGF-A pathway induces neoangiogenesis with peritoneal fibrosis in patients undergoing peritoneal dialysis Reviewed International journal Open Access

Kariya Tetsuyoshi, Nishimura Hayato, Mizuno Masashi, Suzuki Yasuhiro, Matsukawa Yoshihisa, Sakata Fumiko, Maruyama Shoichi, Takei Yoshifumi, Ito Yasuhiko

AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY Vol. 314 ( 2 ) page： F167 - F180 2018.2

Current Status of Peritoneal Dialysis in Japan. Reviewed International coauthorship International journal

Ito Y, Tawada M, Tine S, Mizuno M, Suzuki Y, Katsuno T

Contributions to nephrology Vol. 196 page： 123 - 128 2018

Peritoneal eosinophils increase during induction of peritoneal dialysis and may be related to production of C3a in peritoneal dialysate

Mizuno Masashi, Shigemoto Emi, Kobayashi Kazuma, Iguchi Daiki, Sakata Fumiko, Suzuki Yasuhiro, Maruyama Shoichi, Ito Yasuhiko

MOLECULAR IMMUNOLOGY Vol. 89 page： 193-193 2017.9

Establishment of a complement examination system for complement-related diseases by the Japanese Association for Complement Research (JACR) International journal

Yoshihiko Hidaka, Norimitsu Inoue, Yasufumi Ohtsuka, Toshihiro Sawai, Toshiyuki Miyata, Isao Osawa, Katsuki Ohtani, Hidechika Okada, Taroh Kinoshita, Hideharu Sekine, Hiroshi Tsukamoto, Miki Nakao, Masashi Mizuno, Yoshiko Murakami, Takahiko Horiuchi, Nobutaka Wakamiya

MOLECULAR IMMUNOLOGY Vol. 89 page： 192 - 192 2017.9

Hexamerization-enhanced CD20 antibody mediates complement-dependent cytotoxicity in serum genetically deficient in C9 Reviewed International coauthorship International journal Open Access

Ronald P. Taylor, Margaret A. Lindorfer, Erika M. Cook, Frank J. Beurskens, Janine Schuurman, Paul W. H. I. Parren, Clive S. Zent, Karl R. VanDerMeid, Richard Burack, Masashi Mizuno, B. Paul Morgan

CLINICAL IMMUNOLOGY Vol. 181 page： 24 - 28 2017.8

Language：English Publishing type：Research paper (scientific journal) Publisher：ACADEMIC PRESS INC ELSEVIER SCIENCE

We examined complement-dependent cytotoxicity (CDC) by hexamer formation-enhanced CD20 mAb Hx-7D8 of patient-derived chronic lymphocytic leukemia (CLL) cells that are relatively resistant to CDC. CDC was analyzed in normal human serum (NHS) and serum from an individual genetically deficient for C9. Hx-7D8 was able to kill up to 80% of CLL cells in complete absence of C9. We conclude that the narrow C5b-8 pores formed without C9 are sufficient for CDC due to efficient antibody-mediated hexamer formation. In the absence of C9, we observed transient intracellular increases of Ca2+ during CDC (as assessed with FLUO-4) that were extended in time. This suggests that small C5b-8 pores allow Ca2+ to enter the cell, while dissipation of the fluorescent signal accompanying cell disintegration is delayed. The Ca2+ signal is retained concomitantly with TOPRO-3 (viability dye) staining, thereby confirming that Ca2+ influx represents the most proximate mediator of cell death by CDC. (C) 2017 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.clim.2017.05.016

Apoptosis inhibitor of macrophage ameliorates fungus-induced peritoneal injury model in mice Reviewed International journal Open Access

Takako Tomita, Satoko Arai, Kento Kitada, Masashi Mizuno, Yasuhiro Suzuki, Fumiko Sakata, Daisuke Nakano, Emiri Hiramoto, Yoshifumi Takei, Shoichi Maruyama, Akira Nishiyama, Seiichi Matsuo, Toru Miyazaki, Yasuhiko Ito

SCIENTIFIC REPORTS Vol. 7 ( 1 ) page： 6450 2017.7

Language：English Publishing type：Research paper (scientific journal) Publisher：NATURE PUBLISHING GROUP

Fungal peritonitis in a patient on peritoneal dialysis (PD) is a refractory injury accompanied by severe inflammation, predisposing patients to a poor prognosis. Defective clearance of necrotic tissue interferes with amelioration of tissue injury and induces abnormal tissue remodeling. In the recent reports, apoptosis inhibitor of macrophage (AIM, also called CD5L) prevents obesity, hepatocellular carcinoma and acute kidney injury. Here, we investigated potential roles of AIM in prevention of progression of fungal peritonitis models. AIM(-/)-mice subjected to zymosan-induced peritonitis exhibited progressive inflammation and sustained peritoneal necrosis tissue on day 28 after the disease induction, whereas there was an improvement in AIM(+/+) mice. This appeared to be caused by deposition of AIM at the necrotic peritoneum in AIM(+/+) mice. In vitro, AIM enhanced the engulfment of necrotic debris by macrophages derived from zymosan-induced peritonitis, M1-and M2a-like bone marrow derived macrophages, as well as by mesothelial cells. In addition, administration of recombinant AIM dramatically ameliorated severe inflammation associated with necrosis in zymosan-induced peritonitis of AIM(-/)-mice. Our observations suggest that AIM appears to be involved in the repair process of zymosan-induced peritonitis, and thus, could be the basis of development of new therapeutic strategies for PD-related fungal peritonitis.

DOI： 10.1038/s41598-017-06824-6

Sodium chloride promotes tissue inflammation via osmotic stimuli in subtotal-nephrectomized mice Reviewed International journal Open Access

Fumiko Sakata, Yasuhiko Ito, Masashi Mizuno, Akiho Sawai, Yasuhiro Suzuki, Takako Tomita, Mitsuhiro Tawada, Akio Tanaka, Akiyoshi Hirayama, Akihiro Sagara, Takashi Wada, Shoichi Maruyama, Tomoyoshi Soga, Seiichi Matsuo, Enyu Imai, Yoshifumi Takei

LABORATORY INVESTIGATION Vol. 97 ( 4 ) page： 432 - 446 2017.4

Language：English Publishing type：Research paper (scientific journal) Publisher：NATURE PUBLISHING GROUP

Chronic inflammation, which is often associated with high all-cause and cardiovascular mortality, is prevalent in patients with renal failure; however, the precise mechanisms remain unclear. High-salt intake was reported to induce lymphangiogenesis and autoimmune diseases via osmotic stimuli with accumulation of sodium or chloride. In addition, sodium was recently reported to be stored in the extremities of dialysis patients. We studied the effects and mechanisms of high salt loading on tissue and systemic inflammation in subtotal-nephrectomized mice (5/6Nx) and in cultured cells. Macrophage infiltration in the peritoneal wall (P<0.001), heart (P<0.05) and para-aortic tissues (P<0.001) was significantly higher in 5/6Nx with salt loading (5/6Nx/NaCl) than in 5/6Nx without salt loading (5/6Nx/Water); however, there were no significant differences in blood pressure and renal function between the groups. Tissue interleukin-6, monocyte chemotactic protein-1 (MCP-1), serum- and glucocorticoid-inducible kinase 1 (Sgk1) and tonicity-responsive enhancer binding protein (TonEBP) mRNA were significantly elevated in the peritoneal wall and heart with 5/6Nx/NaCl when compared with 5/6Nx/Water. Sodium was stored in the abdominal wall, exerting high-osmotic conditions. Reversal of salt loading reduced macrophage infiltration associated with decreased TonEBP in 5/6Nx/NaCl. Macrophage infiltration associated with fibrosis induced by salt loading was decreased in the 5/6Nx/NaCl/CC chemokine receptor 2 (CCR2, receptor of MCP-1)-deficient mice when compared with 5/6Nx/NaCl/Wild mice, suggesting that CCR2 is required for macrophage infiltration in 5/6Nx with NaCl loading. In cultured mesothelial cells and cardiomyocytes, culture media with high NaCl concentration induced MCP-1, Sgk1 and TonEBP mRNA, all of which were suppressed by TonEBP siRNA, indicating that both MCP-1 and Sgk1 are downstream of TonEBP. Our study indicates that high NaCl intake induces MCP-1 expression leading to macrophage infiltration via the TonEBP-MCP-1 pathway in 5/6Nx/NaCl mice, and that TonEBP has a central role in inflammation in patients with renal failure taking high salt.

DOI： 10.1038/labinvest.2017.4

Web of Science

PubMed

researchmap

Tetracycline Reduces Kidney Damage Induced by Loxosceles Spider Venom Reviewed International coauthorship International journal Open Access

Okamoto, CK; Van den Berg, CW; Masashi, M; Gonçalves-de-Andrade, RM; Tambourgi, DV

TOXINS Vol. 9 ( 3 ) 2017.3

Complement component 5 promotes lethal thrombosis International journal Open Access

Mizuno Tomohiro, Yoshioka Kengo, Mizuno Masashi, Shimizu Mie, Nagano Fumihiko, Okuda Tomoyuki, Tsuboi Naotake, Maruyama Shoichi, Nagamatsu Tadashi, Imai Masaki

SCIENTIFIC REPORTS Vol. 7 page： 42714 2017.2

　 PREV - NEXT 　

<MIZUNO, Masashi>

Papers - MIZUNO, Masashi