Faculty Profiles - UCHIDA Hiroo

Postoperative intestinal obstruction in patients with biliary atresia impedes biliary excretion and results in subsequent liver transplantation (vol 37, pg 229, 2021) Reviewed

Takimoto Aitaro, Sumida Wataru, Amano Hizuru, Shirota Chiyoe, Tainaka Takahisa, Yokota Kazuki, Makita Satoshi, Yasui Akihiro, Kanou Yoko, Hinoki Akinari, Uchida Hiroo

PEDIATRIC SURGERY INTERNATIONAL Vol. 37 ( 6 ) page： 835 - 835 2021.6

NOVEL BIOMARKER DISCOVERY FOR CHILDHOOD RHABDOMYOSARCOMA USING URINARY METABOLITES Reviewed

Amano Hizuru, Hinoki Akinari, Uchida Hiroo, Yokota Kazuki, Ishigaki Takashi, Sakairi Minoru, Abe Mayumi, Terui Yasushi, Oda Hirohisa, Tainaka Takahisa, Shirota Chiyoe, Sumida Wataru, Makita Satoshi, Takimoto Aitaro, Kano Yoko, Okamoto Masamune, Yasui Akihiro, Nakagawa Yoichi

PEDIATRIC BLOOD & CANCER Vol. 68 page： S46 - S46 2021.6

胆道閉鎖症におけるオキシステロール及び胆汁酸分析の有用性 a pilot study Reviewed

小西健一郎, 水落建輝, 橋詰直樹, 八木実, 松浦俊治, 田口智章, 眞田幸弘, 田井中貴久, 内田広夫, 佐々木英之, 仁尾正記, 藤代準, 武井一, 入戸野博, 木村昭彦

日本小児外科学会雑誌 Vol. 57 ( 4 ) page： 786 - 787 2021.6

Double-balloon endoscopic retrograde cholangiography can make a reliable diagnosis and good prognosis for postoperative complications of congenital biliary dilatation Reviewed

Shirota Chiyoe, Kawashima Hiroki, Tainaka Takahisa, Sumida Wataru, Yokota Kazuki, Makita Satoshi, Amano Hizuru, Takimoto Aitaro, Hinoki Akinari, Uchida Hiroo

SCIENTIFIC REPORTS Vol. 11 ( 1 ) page： 11052 2021.5

Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Scientific Reports

Bile duct and anastomotic strictures and intrahepatic stones are common postoperative complications of congenital biliary dilatation (CBD). We performed double-balloon endoscopic retrograde cholangiography (DBERC) for diagnostic and therapeutic purposes after radical surgery. We focused on the effectiveness of DBERC for the treatment of postoperative complications of CBD patients. Bile duct and anastomotic strictures and intrahepatic stones are common postoperative complications of congenital biliary dilatation (CBD). We performed double-balloon endoscopic retrograde cholangiography (DBERC) for diagnostic and therapeutic purposes after radical surgery. We focused on the effectiveness of DBERC for the treatment of postoperative complications of CBD patients. This retrospective study included 28 patients who underwent DBERC (44 procedures) after radical surgery for CBD between January 2011 and December 2019. Strictures were diagnosed as “bile duct strictures” if endoscopy confirmed the presence of bile duct mucosa between the stenotic and anastomotic regions, and as “anastomotic strictures” if the mucosa was absent. The median patient age was 4 (range 0–67) years at the time of primary surgery for CBD and 27.5 (range 8–76) years at the time of DBERC. All anastomotic strictures could be treated with only by 1–2 courses of balloon dilatation of DBERC, while many bile duct strictures (41.2%) needed ≥ 3 treatments, especially those who underwent operative bile duct plasty as the first treatment (83.3%). Although the study was limited by the short follow-up period after DBERC treatment, DBERC is recommended as the first-line treatment for hepatolithiasis associated with biliary and anastomotic strictures in CBD patients, and it can be safely performed multiple times.

DOI： 10.1038/s41598-021-90550-7

A Retrospective Study on the Effect of Surgical Experiences of Laparoscopic Kasai Portenterostomy Reviewed

特集シミュレーションとナビゲーション精索静脈瘤 Reviewed

田井中貴久, 田中裕次郎, 檜顕成, 城田千代栄, 住田亙, 横田一樹, 牧田智, 内田広夫

小児外科 Vol. 53 ( 5 ) page： 560 - 563 2021.5

Urinary biomarkers for monitoring treatment response in neuroblastoma patients Reviewed

Hiroo UCHIDA

Journal of Clinical Oncology Vol. 39 ( 15 ) 2021.5

Kurano Yui, Shirota Chiyoe, Hinoki Akinari, Tainaka Takahisa, Sumida Wataru, Yokota Kazuki, Makita Satoshi, Takimoto Aitaro, Uchida Hiroo

Journal of the Japanese Society of Pediatric Surgeons Vol. 57 ( 3 ) page： 596 - 599 2021.4

Language：Japanese Publishing type：Research paper (scientific journal) Publisher：The Japanese Society of Pediatric Surgeons

Purpose: The treatment outcomes of patients with laparoscopic surgery for biliary atresia (laparoscopic portoenterostomy) were retrospectively evaluated to determine whether to limit the laparoscopic surgery for biliary atresia to fully experienced surgeons.Methods: We included 37 patients with laparoscopic portoenterostomy for biliary atresia at our institution from 2014 to 2018. Twelve patients were treated by a surgeon fully experienced in laparoscopic portoenterostomy (Group A) and 23 patients were treated by other surgeons (Group B). Jaundice reduction and jaundice-free survival of patients with the native liver in the two groups were compared.Results: Differences in age at the time of surgery, body weight, perioperative blood loss, time of drain removal, and time that milk or formula feeding resumed in both groups were not significant. The duration of surgery was significantly shorter in Group A than in Group B. The jaundice-free survival rates of patients with the native liver were 58.3% in Group A and 60.9% in Group B at 6 months after surgery, 66.7% in Group A and 65.2% in Group B at 1 year after surgery, and 58.3% in Group A and 56.5% in Group B at 2 years after surgery. No significant difference was observed.Conclusions: In this study, no significant difference in the rate of jaundice-free survival of patients with the native liver depending on the amount of surgical experience was observed under the guidance of an operator who was skilled in laparoscopic portoenterostomy.

DOI： 10.11164/jjsps.57.3_596

CiNii Research

A Japanese prospective multicenter study of urinary oxysterols in biliary atresia Reviewed

Konishi Ken-ichiro, Mizuochi Tatsuki, Takei Hajime, Yasuda Ryosuke, Sakaguchi Hirotaka, Ishihara Jun, Takaki Yugo, Kinoshita Masahiro, Hashizume Naoki, Fukahori Suguru, Shoji Hiromichi, Miyano Go, Yoshimaru Koichiro, Matsuura Toshiharu, Sanada Yukihiro, Tainaka Takahisa, Uchida Hiroo, Kubo Yumiko, Tanaka Hiromu, Sasaki Hideyuki, Murai Tsuyoshi, Fujishiro Jun, Yamashita Yushiro, Nio Masaki, Nittono Hiroshi, Kimura Akihiko

SCIENTIFIC REPORTS Vol. 11 ( 1 ) page： 4986 2021.3

Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Scientific Reports

Diagnosis of biliary atresia (BA) can involve uncertainties. In the present prospective multicenter study, we considered whether urinary oxysterols represent a useful marker for diagnosis of BA in Japanese children. Subjects under 6 months old at 7 pediatric centers in Japan were prospectively enrolled, including patients with cholestasis and healthy controls (HC) without liver disease. Patients with cholestasis constituted 2 groups representing BA patients and others with cholestasis from other causes (non-BA). We quantitatively analyzed 7 oxysterols including 4β-, 20(S)-, 22(S)-, 22(R)-, 24(S)-, 25-, and 27-hydroxycholesterol by liquid chromatography/electrospray ionization-tandem mass spectrometry. Enrolled subjects included 14 with BA (median age 68 days; range 26–170) and 10 non-BA cholestatic controls (59; 14–162), as well as 10 HC (57; 25–120). Total urinary oxysterols were significantly greater in BA (median, 153.0 μmol/mol creatinine; range 24.1–486.7; P < 0.001) and non-BA (36.2; 5.8–411.3; P < 0.05) than in HC (2.7; 0.8–7.6). In patients with BA, urinary 27-hydroxycholesterol (3.61; 0.42–11.09; P < 0.01) was significantly greater than in non-BA (0.71; 0–5.62). In receiver operating characteristic (ROC) curve analysis for distinguishing BA from non-BA, the area under the ROC curve for urinary 27-hydroxycholesterol was 0.83. In conclusion, this first report of urinary oxysterol analysis in patients with BA indicated that 27-hydroxycholesterol may be a useful marker for distinguishing BA from other causes of neonatal cholestasis.

DOI： 10.1038/s41598-021-84445-w

Identification of novel neuroblastoma biomarkers in urine samples Reviewed

Yokota Kazuki, Uchida Hiroo, Sakairi Minoru, Abe Mayumi, Tanaka Yujiro, Tainaka Takahisa, Shirota Chiyoe, Sumida Wataru, Oshima Kazuo, Makita Satoshi, Amano Hizuru, Hinoki Akinari

SCIENTIFIC REPORTS Vol. 11 ( 1 ) page： 4055 2021.2

Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Scientific Reports

Urine is a complex liquid containing numerous small molecular metabolites. The ability to non-invasively test for cancer biomarkers in urine is especially beneficial for screening child patients. This study attempted to identify neuroblastoma biomarkers by comprehensively analysing urinary metabolite samples from children. A total of 87 urine samples were collected from 54 participants (15 children with neuroblastoma and 39 without cancer) and used to perform a comprehensive analysis. Urine metabolites were extracted using liquid chromatography/mass spectrometry and analysed by Metabolon, Inc. Biomarker candidates were extracted using the Wilcoxon rank sum test, random forest method (RF), and orthogonal partial least squares discriminant analysis (OPLS-DA). RF identified three important metabolic pathways in 15 samples from children with neuroblastoma. One metabolite was selected from each of the three identified pathways and combined to create a biomarker candidate (3-MTS, CTN, and COR) that represented each of the three pathways; using this candidate, all 15 cases were accurately distinguishable from the control group. Two cases in which known biomarkers were negative tested positive using this new biomarker. Furthermore, the predictive value did not decrease in cases with a low therapeutic effect. This approach could be effectively applied to identify biomarkers for other cancer types.

DOI： 10.1038/s41598-021-83619-w

Risk factors and outcomes of bile leak after laparoscopic surgery for congenital biliary dilatation Reviewed International journal

Tanaka Yujiro, Tainaka Takahisa, Hinoki Akinari, Shirota Chiyoe, Sumida Wataru, Yokota Kazuki, Oshima Kazuo, Makita Satoshi, Amano Hizuru, Takimoto Aitaro, Kano Yoko, Uchida Hiroo

PEDIATRIC SURGERY INTERNATIONAL Vol. 37 ( 2 ) page： 235 - 240 2021.2

Thoracoscopic surgery for congenital lung cysts: an attempt to limit pulmonary resection in cases of lesions involving multiple lobes Reviewed International journal

Shirota Chiyoe, Tainaka Takahisa, Sumida Wataru, Yokota Kazuki, Makita Satoshi, Takimoto Aitaro, Amano Hizuru, Hinoki Akinari, Ono Yasuyuki, Uchida Hiroo

PEDIATRIC SURGERY INTERNATIONAL Vol. 37 ( 2 ) page： 213 - 221 2021.2

Usefulness of web search queries for early detection of diseases in infants Reviewed

Yamaguchi Shuji, Hinoki Akinari, Tsubouchi Kota, Amandu Hizuru, Tajima Akira, Uchida Hiroo

NAGOYA JOURNAL OF MEDICAL SCIENCE Vol. 83 ( 1 ) page： 107 - 111 2021.2

Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Nagoya Journal of Medical Science

Early detection of diseases is critical in infants. This study evaluates the usefulness of web searches in predicting diseases in order to encourage guardians to consult a doctor promptly if their children are ill. We collected six months of search queries from Yahoo! JAPAN Search between October 2016 and March 2017. Using a machine learning model, we investigated the accuracy of the search query’s ability to predict the diagnosis of biliary atresia and hypertrophic pyloric stenosis. Both diseases were modeled with an accuracy of approximately 80&, and symptoms related to the disease were significant features in the model. These findings suggest the possibility of detecting diseases from web search queries performed by guardians. Through future research, we intend to propose a method that uses web search queries for early detection of these diseases by providing appropriate and timely information to support the guardians of patients.

DOI： 10.18999/nagjms.83.1.107