研究者詳細 - 阿部　洋

論文 - 阿部　洋

分割表示 248 件中 141 - 160 件目／全件表示 >>

In vitro selection of a photo-responsive peptide aptamer using ribosome display 査読有り

Liu Mingzhe, Tada Seiichi, Ito Mika, Abe Hiroshi, Ito Yoshihiro

CHEMICAL COMMUNICATIONS 48 巻 ( 97 ) 頁： 11871 - 11873 2012年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Chemical Communications

A photo-responsive peptide aptamer against microbeads immobilized streptavidin was isolated using in vitroselection combined with photo-manipulation. This is the first example of the introduction of a peptide aptamer in the photo-control of dynamic molecular recognition. © 2012 The Royal Society of Chemistry.

DOI： 10.1039/c2cc36618e

Tuning efficiency of the 4-exo-trig cyclization by the electronic effect: ring closure of 3,3-difluoro-4-pentenyl carbon radicals and synthesis of a gem-difluorocyclobutane nucleoside 査読有り

Kumamoto Hiroki, Kawahigashi Sachiko, Wakabayashi Hiromi, Nakano Tomohiko, Miyaike Tomoko, Kitagawa Yasuyuki, Abe Hiroshi, Ito Mika, Haraguchi Kazuhiro, Balzarini Jan, Baba Masanori, Tanaka Hiromichi

CHEMICAL COMMUNICATIONS 48 巻 ( 89 ) 頁： 10993 - 10995 2012年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Chemical Communications

4-exo-trig Cyclization reaction of a 4-pentenyl carbon radical containing the gem-difluoromethylene moiety adjacent to a radical accepting α,β-unsaturated ester was found to proceed efficiently to furnish a novel gem-difluorocyclobutane derivative. The cyclized product could be transformed into a gem-difluoromethylene analogue of oxetanocin T. © 2012 The Royal Society of Chemistry.

DOI： 10.1039/c2cc35876j

Synthesis of dumbbell-shaped cyclic RNAs for RNA interference. 査読有り

Abe N, Abe H, Ito Y

Current protocols in nucleic acid chemistry 1 巻 ( 48 ) 頁： 16.4.11 - 11 2012年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Current Protocols in Nucleic Acid Chemistry

RNA interference (RNAi) is a potent and highly specific gene-silencing phenomenon that was first reported for the nematode Caenorhabditis elegans. It has been discovered that genes could be silenced by introducing double-stranded RNAs (dsRNAs) complementary to the messenger RNA sequences. Since then, RNAi has been shown as an evolutionarily well-conserved process that plays an important role in host defense and in regulation of gene expression. Much effort has been dedicated to the application of the short dsRNA species (short interfering RNAs; siRNAs) as therapeutic agents, as they were shown to be effective in mammalian cells. Recently, we altered the structure of a siRNA molecule and produced dumbbell-shaped nanocircular RNAs. RNA dumbbells were shown to be stabilized in serum compared with its siRNA counterpart, despite their natural RNA strand. It has also been found that RNA dumbbells containing a 23-bp stem and two 9-nt loops exhibit a prolonged RNAi effect in cultured mammalian cells. In this unit, we describe the synthesis of RNA dumbbells from the design, its enzymatic synthesis, and to the purification. © 2012 by John Wiley & Sons, Inc.

DOI： 10.1002/0471142700.nc1604s48

Structure Formation and Catalytic Activity of DNA Dissolved in Organic Solvents 査読有り

Abe Hiroshi, Abe Naoko, Shibata Aya, Ito Keiji, Tanaka Yoshiyuki, Ito Mika, Saneyoshi Hisao, Shuto Satoshi, Ito Yoshihiro

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 51 巻 ( 26 ) 頁： 6475 - 6479 2012年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Angewandte Chemie - International Edition

Equal-opportunity dissolver: By attaching polyethylene glycol at its 5' end, DNA (PEG-DNA) can be solubilized in various organic solvents and was shown to form G-quadruplexes by CD spectroscopy. A complex containing iron(III) protoporphyrin IX (hemin) and G-quadruplex-forming PEG-DNA catalyzed an oxidative reaction in methanol (see scheme). Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

DOI： 10.1002/anie.201201111

Positively charged cholesterol-recombinant human gelatins foster the cellular uptake of proteins and murine immune reactions 査読有り

Kadengodlu Pallavi A., Hebishima Takehisa, Takeshima Shin-Nosuke, Ito Mika, Liu Mingzhe, Abe Hiroshi, Aida Yoko, Aigaki Toshiro, Ito Yoshihiro

INTERNATIONAL JOURNAL OF NANOMEDICINE 7 巻頁： 5437 - 5450 2012年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：International Journal of Nanomedicine

Purpose: Recombinant human gelatins with defined molecular weights were modified with cholesterol to make them amphiphilic in nature. We investigated the feasibility of these modified human gelatins acting as a carrier of antigenic proteins for inducing cellular immunity. The aim of this study was to synthesize novel and effective compounds for vaccine delivery in vivo. Methods: Two types of cholesterol-modified gelatin micelles, anionic cholesterol-modified gelatin (aCMG) and cationic-cholesterol modified gelatin (cCMG), were synthesized using different cholesterol derivatives such as the cholesterol-isocyanate (Ch-I) for aCMG and amino-modified cholesterol for cCMG. One was anionic and the other cationic, and therefore they differed in terms of their zeta potential. The aCMG and cCMG were characterized for their size, zeta potential, and in their ability to form micelles. Cytotoxicity was also evaluated. The modified human gelatins were then investigated as a carrier of antigenic proteins for inducing cellular immunity both in vitro in DC 2.4 cells, a murine dendritic cell line, as well as in vivo. The mechanism of entry of the polymeric micelles into the cells was also evaluated. Results: It was found that only cCMG successfully complexed with the model antigenic protein, fluorescein-isothiocyanate ovalbumin (OVA) and efficiently delivered and processed proteins in DC 2.4 cells. It was hypothesized that cCMG enter the cells predominantly by a caveolae-mediated pathway that required tyrosine kinase receptors on the cell surface. Animal testing using mice showed that the cationic cholesterol-modified gelatin complexed with OVA produced significantly high antibody titers against OVA: 2580-fold higher than in mice immunized with free OVA. Conclusion: Conclusively, cCMG has shown to be very effective in stimulating an immune response due to its high efficiency, stability, and negligible cytotoxicity. © 2012 Cárdenas et al, publisher and licensee Dove Medical Press Ltd.

DOI： 10.2147/IJN.S36350

Synthesis, structure, and biological activity of dumbbell-shaped nanocircular RNAs for RNA interference. 査読有り

Abe N, Abe H, Nagai C, Harada M, Hatakeyama H, Harashima H, Ohshiro T, Nishihara M, Furukawa K, Maeda M, Tsuneda S, Ito Y

Bioconjugate chemistry 22 巻 ( 10 ) 頁： 2082 - 92 2011年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Bioconjugate Chemistry

RNA interference (RNAi) is one of the most promising new approaches for disease therapy. The design of a dumbbell-shaped nanocircular RNA allows it to act as a short interfering RNA (siRNA) precursor. To optimize the design, we studied the relationship between the nanostructure and RNAi activity by synthesizing various RNA dumbbells. An RNA dumbbell with a 23-bp stem and 9-nt loops was the most potent. Sequence analysis by mass spectrometry showed that Dicer could edit RNA dumbbells to siRNA species. The reaction offered the slow release of siRNA species, which conferred prolonged RNAi activity. Introduction of DNA into the loop position significantly stabilized the dumbbell in biological fluid without any loss of RNAi activity. In-depth pharmacological evaluation was performed by introducing dumbbells into HeLa cells that stably express the target luciferase gene. The dumbbells provided a rapid silencing effect and retained this effect for a longer time even at a lower concentration than that at which standard siRNA completely lost RNAi activity. We conclude that an RNA dumbbell with DNA loops is the most promising design for in vivo applications for RNA medicine. © 2011 American Chemical Society.

DOI： 10.1021/bc2003154

Synthesis, Structure, and Biological Activity of Dumbbell-Shaped Nanocircular RNAs for RNA Interference 査読有り

Abe Naoko, Abe Hiroshi, Nagai Chisato, Harada Mitsuru, Hatakeyama Hiroto, Harashima Hideyoshi, Ohshiro Takahito, Nishihara Mizuki, Furukawa Kazuhiro, Maeda Mizuo, Tsuneda Satoshi, Ito Yoshihiro

BIOCONJUGATE CHEMISTRY 22 巻 ( 10 ) 頁： 2082 - 2092 2011年10月

Synthesis and Characterization of a Series of Highly Fluorogenic Substrates for Glutathione Transferases, a General Strategy 査読有り

Zhang Jie, Shibata Aya, Ito Mika, Shuto Satoshi, Ito Yoshihiro, Mannervik Bengt, Abe Hiroshi, Morgenstern Ralf

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 133 巻 ( 35 ) 頁： 14109 - 14119 2011年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of the American Chemical Society

Glutathione transferases (GSTs) are used in biotechnology applications as fusion partners for facile purification and are also overexpressed in certain tumors. Consequently, there is a need for sensitive detection of the enzymes. Here we describe a general strategy for the synthesis and characterization of novel fluorogenic substrates for GSTs. The substrates were synthesized by introducing an electrophilic sulfonamide linkage to fluorescent molecules containing an amino group [e.g., 2,4-dinitrobenzenesulfonamide (DNs) derivatives of coumarin, cresyl violet, and rhodamine]. The derivatives were essentially nonfluorescent, and upon GST catalyzed cleavage of the dinitrobenzenesulfonamide, free fluorophore is released (and 1-glutathionyl-2,4-dinitrobenzene + SO 2). All the coumarin-, cresyl violet- and rhodamine-based fluorogenic probes turned out to be good substrates for most GSTs, especially for GSTA 1-1, in terms of strong fluorescence increases (71-1200-fold), high k cat/K m values (10 4-10 7 M -1 s -1) and significant rate enhancements (10 6-10 9-fold). The substrates were successfully applied to quantitate very low levels of GST activity in cell extracts and DNs-cresyl violet was also successfully applied to the imaging of microsomal MGST 1 activity in living cells. The cresyl violet stained cells retained their fluorescence after fixation, which is a very useful property. In summary, we describe a general and versatile strategy to generate fluorogenic GST substrates, some of them providing the most sensitive assays so far described for GSTs. © 2011 American Chemical Society.

DOI： 10.1021/ja205500y

Synthesis of 4 '-Ethynyl-2 '-deoxy-4 '-thioribonucleosides and Discovery of a Highly Potent and Less Toxic NRTI 査読有り

Haraguchi Kazuhiro, Shimada Hisashi, Kimura Keigo, Akutsu Genta, Tanaka Hiromichi, Abe Hiroshi, Hamasaki Takayuki, Baba Masanori, Gullen Elizabeth A., Dutschman Ginger E., Cheng Yung-Chi, Balzarini Jan

ACS MEDICINAL CHEMISTRY LETTERS 2 巻 ( 9 ) 頁： 692 - 697 2011年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ACS Medicinal Chemistry Letters

The synthesis of 4′-ethynyl-2′-deoxy-4′- thioribonucleosides was carried out utilizing an electrophilic glycosidation in which 4-ethynyl-4-thiofuranoid glycal 16 served as a glycosyl donor. Electrophilic glycosidation between 16 and the silylated nucleobases (N 4-acetylcytosine, N 6-benzoyladenine, and N 2-acetyl-O 6-diphenylcarbamoylguanine) was carried out in the presence of N-iodosuccinimide (NIS), leading to the exclusive formation of the desired β-anomers 29, 33, and 36. Anti-HIV studies demonstrated that these 4′-thio nucleosides were less cytotoxic to T-lymphocyte (i.e., MT-4 cells) than the corresponding 4′-ethynyl derivatives of 2′-deoxycytidine (44), 2′-deoxyadenosine (45), and 2′-deoxyguanosine (46). Comparison of the selectivity indices (SI) was made between 4′-thionucleosides (32, 41, and 43) and the corresponding 4′-oxygen analogues 44-46 by using the reported CC 50 and EC 50 values. In the case of cytosine and adenine nucleosides, comparable SI values were obtained as follows: 32 (545) and 44 (458); 41 (>230) and 45 (1630). In contrast, 4′-ethynyl-2′-deoxy-4′- thioguanosine 43 was found to possess a SI value of >18200, which is 20 times better than that of 46 (933). © 2011 American Chemical Society.

DOI： 10.1021/ml2001054

PEGylated antibody in organic media 査読有り

Liu Mingzhe, Xu Muye, Loh Xian Jun, Abe Hiroshi, Tsumuraya Takeshi, Fujii Ikuo, Li Jun, Son Tae Il, Ito Yoshihiro

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 111 巻 ( 5 ) 頁： 564 - 568 2011年5月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of Bioscience and Bioengineering

Antibodies were covalently conjugated with poly(ethylene glycol) (PEG) and the properties of the PEGylated antibodies in organic media were investigated. Two types of monoclonal antibody were used in this study. One was a monoclonal antibody (abzyme) that was prepared against a hapten mimicking a transition state of hydrolysis. Another was a monoclonal antibody against estrogen, which is not soluble in water. By electrophoresis and mass spectral analysis, the covalent conjugation with PEG chains was confirmed. The PEGylated antibodies bound to antigens and the PEGylated abzyme catalyzed a hydrolysis reaction in an aqueous solution. The PEGylated antibodies were soluble in dichloromethane and acetone and interacted with antigen either in dichloromethane or in acetone. In conclusion, PEGylated antibodies can be employed as analytical tools for water-insoluble analytes. © 2011 The Society for Biotechnology, Japan.

DOI： 10.1016/j.jbiosc.2011.01.001

Indomethacin Analogues that Enhance Doxorubicin Cytotoxicity in Multidrug Resistant Cells without Cox Inhibitory Activity 査読有り

Arisawa Mitsuhiro, Kasaya Yayoi, Obata Tohru, Sasaki Takuma, Ito Mika, Abe Hiroshi, Ito Yoshihiro, Yamano Akihito, Shutot Satoshi

ACS MEDICINAL CHEMISTRY LETTERS 2 巻 ( 5 ) 頁： 353 - 357 2011年5月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ACS Medicinal Chemistry Letters

Conformationally restricted indomethacin analogues were designed and prepared from the corresponding 2-substituted indoles, which were synthesized by a one-pot isomerization/enamide-ene metathesis as the key reaction. Conformational analysis by calculations, NMR studies, and X-ray crystallography suggested that these analogues were conformationally restricted in the s-cis or the s-trans form due to the 2-substituent as expected. Their biological activities on cyclooxygenase-1 (COX-1) inhibition, cyclooxygenase-2 (COX-2) inhibition, and modulation of MRP-1-mediated multidrug resistance (MDR) are described. Some of these indomethacin analogues enhanced doxorubicin cytotoxicity, although they do not have any COX inhibitory activity, which suggests that the MDR-modulating effect of an NSAID can be unassociated with its COX-inhibitory activity. This may be an entry into the combination chemotherapy of doxorubicin with a MDR modulator. © 2011 American Chemical Society.

DOI： 10.1021/ml100292y

Creation of Polymer Catalysts by Molecular Evolutionary Engineering and Hybridization of Biocatalysts 査読有り

Mingzhe Liu, Hiroshi Abe, Yoshihiro Ito

KOBUNSHI RONBUNSHU 68 巻 ( 7 ) 頁： 405 - 416 2011年

記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：SOC POLYMER SCIENCE JAPAN

We developed a new type of oligonucleotide-based catalysts which can be called DNAzyme, ribozyme, or aptazyme. They were in vitro selected from a random sequence library of DNA or RNA for binding hemin. The selected oligonucleotides exhibited peroxidase activity by forming a complex with hemin. In addition, an azobenzene-containing oligonucleotide which bound to hemin was also selected. The binding and the catalytic activity was controlled by photo-irradiation. Subsequently, hybridized biocatalysts with synthetic polymers were synthesized. Polyethylene glycol-conjugated antibodies and oligonucleotides were synthesized and it was demonstrated that they were active in organic media. A photo-responsive polymer was also conjugated with an enzyme and its catalytic activity was controlled in organic media.

Fluorescence Detection of Intron Lariat RNA with Reduction-Triggered Fluorescent Probes 査読有り

Furukawa Kazuhiro, Abe Hiroshi, Tamura Yasutsugu, Yoshimoto Rei, Yoshida Minoru, Tsuneda Satoshi, Ito Yoshihiro

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 50 巻 ( 50 ) 頁： 12020 - 12023 2011年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Angewandte Chemie - International Edition

Fluorescence through proximity: Reduction-triggered fluorescent probes bearing either a fluorescein derivative or triphenylphosphine bind sequence-specifically at the branch site of lariat RNA. The chemical reaction between the two substrates leads to the formation of unquenched fluorescein and the emission of a fluorescence signal (see picture). Prelariat and lariat structures can be distinguished by using this method. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

DOI： 10.1002/anie.201104425

Synthesis and characterization of small circular double-stranded RNAs 査読有り

Abe Naoko, Abe Hiroshi, Ohshiro Takahito, Nakashima Yuko, Maeda Mizuo, Ito Yoshihiro

CHEMICAL COMMUNICATIONS 47 巻 ( 7 ) 頁： 2125 - 2127 2011年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Chemical Communications

Small circular double-stranded RNAs (dsRNAs) were synthesized. The structural analysis by atomic force microscopy gave direct images for the interpretation of the structural strain present in circular dsRNAs. Finally, we demonstrated that circular dsRNA caused RNAi effect in cells. © 2011 The Royal Society of Chemistry.

DOI： 10.1039/c0cc04551a

Branched RNA nanostructures for RNA interference 査読有り

Nakashima Yuko, Abe Hiroshi, Abe Naoko, Aikawa Kyoko, Ito Yoshihiro

CHEMICAL COMMUNICATIONS 47 巻 ( 29 ) 頁： 8367 - 8369 2011年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Chemical Communications

Branched RNAs with three- or four-way junctions were designed by assembling single-stranded RNA for RNA interference. Human Dicer transformed branched RNAs into about 20 base pairs of double-stranded RNA, which is a standard siRNA species. Our tetramer design provides a potent silencing effect over a period of 5 days. © 2011 The Royal Society of Chemistry.

DOI： 10.1039/c1cc11780g

Characterization of New Potential Anticancer Drugs Designed To Overcome Glutathione Transferase Mediated Resistance 査読有り

Johansson Katarina, Ito Mika, Schophuizen Carolien M. S., Thengumtharayil Sherin Mathew, Heuser Vanina D., Zhang Jie, Shimoji Miyuki, Vahter Marie, Wee Han Ang, Dyson Paul J., Shibata Aya, Shuto Satoshi, Yoshihiro Ito, Abe Hiroshi, Morgenstern Ralf

MOLECULAR PHARMACEUTICS 8 巻 ( 5 ) 頁： 1698 - 1708 2011年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Molecular Pharmaceutics

Resistance against anticancer drugs remains a serious obstacle in cancer treatment. Here we used novel strategies to target microsomal glutathione transferase 1 (MGST1) and glutathione transferase pi (GSTP) that are often overexpressed in tumors and confer resistance against a number of cytostatic drugs, including cisplatin and doxorubicin (DOX). By synthetically combining cisplatin with a GST inhibitor, ethacrynic acid, to form ethacraplatin, it was previously shown that cytosolic GST inhibition was improved and that cells became more sensitive to cisplatin. Here we show that ethacraplatin is easily taken up by the cells and can reverse cisplatin resistance in MGST1 overexpressing MCF7 cells. A second and novel strategy to overcome GST mediated resistance involves using GST releasable cytostatic drugs. Here we synthesized two derivatives of DOX, 2,4-dinitrobenzenesulfonyl doxorubicin (DNS-DOX) and 4-mononitrobenzenesulfonyl doxorubicin (MNS-DOX) and showed that they are substrates for MGST1 and GSTP (releasing DOX). MGST1 overexpressing cells are resistant to DOX. The resistance is partially reversed by DNS-DOX. Interestingly, the less reactive MNS-DOX was more cytotoxic to cells overexpressing MGST1 than control cells. It would appear that, by controlling the reactivity of the prodrug, and thereby the DOX release rate, selective toxicity to MGST1 overexpressing cells can be achieved. In the case of V79 cells, DOX resistance proportional to GSTP expression levels was noted. In this case, not only was drug resistance eliminated by DNS-DOX but a striking GSTP-dependent increase in toxicity was observed in the clonogenic assay. In summary, MGST1 and GSTP resistance to cytostatic drugs can be overcome and cytotoxicity can be enhanced in GST overexpressing cells. © 2011 American Chemical Society.

DOI： 10.1021/mp2000692

進化分子工学による高分子触媒合成と生体高分子のハイブリット化

劉明哲、阿部洋、伊藤嘉浩

高分子論文集 68 巻 ( 7 ) 頁： 405-416 2011年

Creation of Polymer Catalysts by Molecular Evolutionary Engineering and Hybridization of Biocatalysts

Liu Mingzhe, Abe Hiroshi, Ito Yoshihiro

KOBUNSHI RONBUNSHU 68 巻 ( 7 ) 頁： 405 - 416 2011年

出版者・発行元：Kobunshi Ronbunshu

We developed a new type of oligonucleotide-based catalysts which can be called DNAzyme, ribozyme, or aptazyme. They were in vitro selected from a random sequence library of DNA or RNA for binding hemin. The selected oligonucleotides exhibited peroxidase activity by forming a complex with hemin. In addition, an azobenzene-containing oligonucleotide which bound to hemin was also selected. The binding and the catalytic activity was controlled by photo-irradiation. Subsequently, hybridized biocatalysts with synthetic polymers were synthesized. Polyethylene glycol-conjugated antibodies and oligonucleotides were synthesized and it was demonstrated that they were active in organic media. A photo-responsive polymer was also conjugated with an enzyme and its catalytic activity was controlled in organic media. © 2011, The Society of Polymer Science Japan.

DOI： 10.1295/koron.68.405

Synthesis and properties of a novel nucleoside derivative possessing a 2,3,5,6-tetraazabenzo[cd]azulene skeleton 査読有り

Hirama Yasuyuki, Abe Hiroshi, Minakawa Noriaki, Matsuda Akira

TETRAHEDRON 66 巻 ( 43 ) 頁： 8402 - 8406 2010年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Tetrahedron

We describe herein the synthesis and properties of the novel nucleoside derivative, 4,7-diamino-2-(2-deoxy-β-d-erythro-pentofuranosyl)-2,6-dihydro- 7H-2,3,5,6-tetraazabenzo[cd]azulene (1). The palladium catalyzed cross-coupling reaction of 2,4-diamino-5-iodo-7-(2-deoxy-β-d-erythro-pentofuranosyl) pyrrolo[2,3-d]pyrimidine (9) with acrylonitrile afforded 2,4-diamino-5-[(E)-1- cyano-2-ethenyl]-7-(2-deoxy-β-d-erythro-pentofuranosyl)pyrrolo[2,3-d] pyrimidine (10) in 77% yield, which was treated with NaOMe in MeOH in the presence of NaSPh to give the desired 1 in 64% yield. Whereas 1 was stable in concentrated ammonia at room temperature, it was gradually hydrolyzed in water to give 4-amino-2-(2-deoxy-β-d-erythro-pentofuranosyl)-2,6-dihydro-7H-2,3, 5,6-tetraazabenzo[cd]azulen-7-one (12). Density functional calculations indicated that 12 was 20 kcal/mol more thermodynamically stable than 1 in a model study. © 2010 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.tet.2010.08.063

Hemin-binding aptamers and aptazymes

Liu M., Abe H., Ito Y.

ACS Symposium Series 1043 巻頁： 111 - 123 2010年8月

出版者・発行元：ACS Symposium Series

Aptamers are oligonucleotides that have been engineered through repeated rounds of in vitro selection or by the process of Systematic Evolution of Ligands by EXponential enrichment (SELEX) to bind to various molecular targets such as small molecules, proteins, nucleic acids, and even cells and tissues. Aptamers offer similar molecular recognition properties to the more commonly used antibodies. In addition to their ability to discriminate their target, aptamers offer some advantages over antibodies. For example, aptamers can be engineered completely in a test tube and are readily produced using chemical processes. On the other hand, some aptamers that have been developed exhibit enzyme activity after binding to their target molecule, and function as 'aptazymes'. Numerous aptamers and aptazymes have been used in biotechnology, diagnostics and therapy. In this study, we demonstrate that natural DNA/RNA aptamers produced by selection in vitro, not only bind to hemin, but also show peroxidase activity when in such complexes (i.e., they function as aptazymes). © 2010 American Chemical Society.

DOI： 10.1021/bk-2010-1043.ch009

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