研究者詳細 - 阿部　洋

論文 - 阿部　洋

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Structural optimization of pseudorotaxane-forming oligonucleotides for efficient and stable complex formation

Onizuka Kazumitsu, Miyashita Takuya, Chikuni Tomoko, Ozawa Mamiko, Abe Hiroshi, Nagatsugi Fumi

NUCLEIC ACIDS RESEARCH 46 巻 ( 17 ) 頁： 8710 - 8719 2018年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Nucleic Acids Research

Interlocked structures, such as rotaxane and catenane, combine both static and dynamic properties. To expand their unique properties into the chemical biology field, a spontaneous formation method of the interlocked structures with the target would be ideal. We have previously developed a pseudorotaxane-forming oligo DNA (prfODN) to spontaneously form topological DNA/RNA architectures. In this study, we report the structural optimization of prfODNs for the efficient and stable complex formation. The optimized prfODNs efficiently formed pseudorotaxane structures with a DNA or RNA target, and the yield for the RNA target reached 85% in 5 min. In addition, the optimized prfODNs could form the pseudorotaxane structure with a smaller ring size and the structure significantly increased the kinetic stability. Furthermore, the catenane structure was successfully formed with the optimized prfODNs to provide the conclusive evidence for the formation of the threaded structure. This information will be valuable for developing new chemical methods using functional nucleic acids for antisense oligo nucleotides and DNA/RNA nanotechnology.

DOI： 10.1093/nar/gky744

Web of Science

Scopus

PubMed

MGST1, a GSH transferase/peroxidase essential for development and hematopoietic stem cell differentiation

Brautigam Lars, Zhang Jie, Dreij Kristian, Spahiu Linda, Holmgren Arne, Abe Hiroshi, Tew Kenneth D., Townsend Danyelle M., Kelner Michael J., Morgenstern Ralf, Johansson Katarina

REDOX BIOLOGY 17 巻頁： 171 - 179 2018年7月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Redox Biology

We show for the first time that, in contrast to other glutathione transferases and peroxidases, deletion of microsomal glutathione transferase 1 (MGST1) in mice is embryonic lethal. To elucidate why, we used zebrafish development as a model system and found that knockdown of MGST1 produced impaired hematopoiesis. We show that MGST1 is expressed early during zebrafish development and plays an important role in hematopoiesis. High expression of MGST1 was detected in regions of active hematopoiesis and co-expressed with markers for hematopoietic stem cells. Further, morpholino-mediated knock-down of MGST1 led to a significant reduction of differentiated hematopoietic cells both from the myeloid and the lymphoid lineages. In fact, hemoglobin was virtually absent in the knock-down fish as revealed by diaminofluorene staining. The impact of MGST1 on hematopoiesis was also shown in hematopoietic stem/progenitor cells (HSPC) isolated from mice, where it was expressed at high levels. Upon promoting HSPC differentiation, lentiviral shRNA MGST1 knockdown significantly reduced differentiated, dedicated cells of the hematopoietic system. Further, MGST1 knockdown resulted in a significant lowering of mitochondrial metabolism and an induction of glycolytic enzymes, energetic states closely coupled to HSPC dynamics. Thus, the non-selenium, glutathione dependent redox regulatory enzyme MGST1 is crucial for embryonic development and for hematopoiesis in vertebrates.

DOI： 10.1016/j.redox.2018.04.013

Web of Science

Scopus

PubMed

Chemical ligation reaction for oligonucleotides based on electrophilic phosphorothioester 査読有り

Kimura Yasuaki, Maruyama Hideto, Oikawa Ryota, Hayakawa Mayu, Abe Naoko, Tsuji Genichiro, Matsuda Akira, Shuto Satoshi, Ito Yoshihiro, Abe Hiroshi

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 255 巻 2018年3月

Development of glutathione S-transferase (GST) covalent inhibitor

Shishido Yuko, Tomoike Fumiaki, Kimura Yasuaki, Kuwata Keiko, Yano Takato, Fukui Kenji, Fujikawa Haruka, Sekido Yoshitaka, Murakami-Tonami Yuko, Kameda Tomoshi, Shuto Satoshi, Abe Hiroshi

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 255 巻頁： . 2018年3月

Nano structure design for RNA interference

Shu Zhaoma, Abe Naoko, Tomoike Fumiaki, Kimura Yasuaki, Ito Yoshihiro, Abe Hiroshi

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 255 巻頁： . 2018年3月

Chemical ligation reaction for oligonucleotides based on electrophilic phosphorothioester

Kimura Yasuaki, Maruyama Hideto, Oikawa Ryota, Hayakawa Mayu, Abe Naoko, Tsuji Genichiro, Matsuda Akira, Shuto Satoshi, Ito Yoshihiro, Abe Hiroshi

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 255 巻頁： . 2018年3月

Chemical Ligation Reactions of Oligonucleotides for Biological and Medicinal Applications

Abe Hiroshi, Kimura Yasuaki

CHEMICAL & PHARMACEUTICAL BULLETIN 66 巻 ( 2 ) 頁： 117-122 2018年2月

Development of GST (Glutathione S-transferase) covalent inhibitor

Shishido Yuko, Sekido Yoshitaka, Murakami-Tonami Yuko, Abe Hiroshi

CANCER SCIENCE 109 巻頁： 374-374 2018年1月

MGST1, a GSH transferase/peroxidase essential for development and hematopoietic stem cell differentiation 査読有り

Lars Bräutigam, Jie Zhang, Kristian Dreij, Linda Spahiu, Arne Holmgren, Hiroshi Abe, Kenneth D. Tew, Danyelle M. Townsend, Michael J. Kelner, Ralf Morgenstern, Katarina Johansson

Redox Biology 17 巻頁： 171 - 179 2018年

Preparation of Circular RNA In Vitro 査読有り

Abe Naoko, Kodama Ayumi, Abe Hiroshi

CIRCULAR RNAS: METHODS AND PROTOCOLS 1724 巻頁： 181 - 192 2018年

記述言語：英語掲載種別：論文集(書籍)内論文出版者・発行元：Methods in Molecular Biology

This chapter describes a simple and straightforward way to obtain single-stranded circular RNA sequences in vitro. Linear RNA that is phosphorylated at the 5′ end is first prepared by a chemical or enzymatic method, then circularized using ligase. The function of the prepared circular RNA molecule, such as an ability to induce translation, can then be investigated.

DOI： 10.1007/978-1-4939-7562-4_15

Chemical Ligation Reactions of Oligonucleotides for Biological and Medicinal Applications.

Abe H, Kimura Y

Chemical & pharmaceutical bulletin 66 巻 ( 2 ) 頁： 117 - 122 2018年

Structural optimization of pseudorotaxane-forming oligonucleotides for efficient and stable complex formation 査読有り

Kazumitsu Onizuka, Takuya Miyashita, Tomoko Chikuni, Mamiko Ozawa, Hiroshi Abe, Fumi Nagatsugi

Nucleic Acids Research 46 巻頁： 8710 - 8719 2018年

グルタチオン-S-トランスフェラーゼを標的とした共有結合性阻害剤の開発

宍戸裕子, 藤川遥加, 友池史明, 木村康明, 桑田啓子, 村上優子, 福井健二, 関戸好孝, 矢野貴人, 周東智, 阿部洋

生命科学系学会合同年次大会 2017年度巻頁： [3P - 0205] 2017年12月

A covalent G-site inhibitor for glutathione S-transferase Pi (GSTP(1-1)) 査読有り

Shishido Yuko, Tomoike Fumiaki, Kimura Yasuaki, Kuwata Keiko, Yano Takato, Fukui Kenji, Fujikawa Haruka, Sekido Yoshitaka, Murakami-Tonami Yuko, Kameda Tomoshi, Shuto Satoshi, Abe Hiroshi

CHEMICAL COMMUNICATIONS 53 巻 ( 81 ) 頁： 11138 - 11141 2017年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Chemical Communications

We herein report the first covalent G-site-binding inhibitor for GST, GS-ESF (1), which irreversibly inhibited the GSTP1-1 function. LC-MS/MS and X-ray structure analyses of the covalently linked GST-inhibitor complex suggested that 1 reacted with Tyr108 of GSTP1-1. The mechanism of covalent bond formation was discussed based on MD simulation results.

DOI： 10.1039/c7cc05829b

Tethered 1,2-Si-Group Migrations in Radical-Mediated Ring Enlargements of Cyclic Alkoxysilanes: An EPR Spectroscopic and Computational Investigation

Walton John C., Kanada Ryutaro, Iwamoto Takeaki, Shuto Satoshi, Abe Hiroshi

JOURNAL OF ORGANIC CHEMISTRY 82 巻 ( 13 ) 頁： 6886-6894 2017年7月

Chemical ligation of oligonucleotides using an electrophilic phosphorothioester 査読有り

Hideto Maruyama, Ryota Oikawa, Mayu Hayakawa, Shono Takamori, Yasuaki Kimura, Naoko Abe, Genichiro Tsuji, Akira Matsuda, Satoshi Shuto, Yoshihiro Ito, Hiroshi Abe

Nucleic Acids Research 45 巻 ( 12 ) 頁： 7042-7048 2017年7月

Enhancement of synergistic gene silencing by RNA interference using branched "3-in-1" trimer siRNA

Nair Baiju G., Zhou Yue, Hagiwara Kyoji, Ueki Masashi, Isoshima Takashi, Abe Hiroshi, Ito Yoshihiro

JOURNAL OF MATERIALS CHEMISTRY B 5 巻 ( 22 ) 頁： 4044-4051 2017年6月

Pseudorotaxane formation via the slippage process with chemically cyclized oligonucleotides

Onizuka Kazumitsu, Chikuni Tomoko, Amemiya Takuya, Miyashita Takuya, Onizuka Kyoko, Abe Hiroshi, Nagatsugi Fumi

NUCLEIC ACIDS RESEARCH 45 巻 ( 9 ) 頁： 5036-5047 2017年5月

Enhancement of synergistic gene silencing by RNA interference using branched “3-in-1” trimer siRNA 査読有り

5 巻頁： 4044-4051 2017年5月

環状RNAを用いたローリングサークル型翻訳反応

阿部奈保子, 阿部洋

生物物理 57 巻 ( 1 ) 頁： 5 - 10 2017年

記述言語：日本語出版者・発行元：一般社団法人日本生物物理学会

<p>In an <i>E. coli</i> cell-free translation system, we found that a circular RNA containing an infinite open reading frame produced more translation product than its linear counterpart by two orders of magnitude, because a ribosome can work more effectively towards the elongation on circular RNA than it can on linear RNA. We then tested circular RNAs containing an infinite open reading frame could be translated in eukaryotic systems, in the absence of any particular element for internal ribosome entry, a poly-A tail, or a cap structure. We found that the circular RNAs also produced long peptides in eukaryotic translation systems, possibly owing to the rolling circle amplification mechanism.</p>

DOI： 10.2142/biophys.57.005

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