研究者詳細 - 阿部　洋

論文 - 阿部　洋

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Development of rapid DNA templated reaction based on nucleophilic aromatic substitution reaction 査読有り

Aya Shibata, Hiroshi Abe, Takanori Uzawa, Yukiko Nakano, Satoshi Shuto, Yoshihiro Ito

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 247 巻 2014年3月

Synthesis of active siRNA species via chemical ligation 査読有り

Hideto Maruyama, Akira Matsuda, Satoshi Shuto, Yoshihiro Ito, Hiroshi Abe

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 247 巻 2014年3月

Triphenylphosphinecarboxamide: an effective reagent for the reduction of azides and its application to nucleic acid detection. 査読有り

Saneyoshi H, Ochikubo T, Mashimo T, Hatano K, Ito Y, Abe H

Organic letters 16 巻 ( 1 ) 頁： 30-3 2014年1月

An intracellular buildup reaction of active siRNA species from short RNA fragments 査読有り

Maruyama Hideto, Nakashima Yuko, Shuto Satoshi, Matsuda Akira, Ito Yoshihiro, Abe Hiroshi

CHEMICAL COMMUNICATIONS 50 巻 ( 11 ) 頁： 1284 - 1287 2014年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Chemical Communications

Here we report a new strategy for the buildup reaction of active siRNA species from short RNA fragments in living cells using a chemical ligation reaction. This strategy could decrease undesired immune responses and provide more latitude for RNAi technology in the design and concentration of introduced RNA compared to traditional siRNA methods. © 2014 The Royal Society of Chemistry.

DOI： 10.1039/c3cc47529h

Transmembrane molecular transport through nanopores formed by protein nanotubes 査読有り

Nair Baiju G., Nakano Yukiko, Ito Yoshihiro, Abe Hiroshi

CHEMICAL COMMUNICATIONS 50 巻 ( 5 ) 頁： 602 - 604 2014年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Chemical Communications

DOI： 10.1039/c3cc45907a

非酵素的に高効率シグナル増幅を可能とする新規RNA検出法を開発

柴田綾、鵜澤尊規、伊藤嘉浩、周東智、阿部洋

化学と生物 52 巻 ( 11 ) 頁： 771-776 2014年

非酵素的に高効率シグナル増幅を可能とする新規RNA検出法を開発

柴田綾, 鵜澤尊規, 伊藤嘉浩, 周東智, 阿部洋

化学と生物 52 巻 ( 11 ) 頁： 771 - 776 2014年

Nanostructured RNAs for RNA interference

Yuko Nakashima, Naoko Abe, Yoshihiro Ito, Hiroshi Abe

Humana Press New York 頁：全 2014年

グルタチオントランスフェラーゼを標的とした分子プローブの開発

伊藤美香,周東智,伊藤嘉浩,阿部洋

有機合成化学教会誌頁： 822 2014年

Polycation-assisted DNA detection by reduction triggered fluorescence amplification probe 査読有り

Saneyoshi Hisao, Shimada Naohiko, Maruyama Atsushi, Ito Yoshihiro, Abe Hiroshi

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 23 巻 ( 24 ) 頁： 6851 - 6853 2013年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Bioorganic and Medicinal Chemistry Letters

We have developed a fluorescence detection system for DNA, assisted by a comb-type cationic polymer (PLL-g-DX), for accelerating the reaction turnover. The combination of fluorogenic DNA probes with a comb-type cationic polymer has been demonstrated to be an effective means of signal amplification during the detection process. The method described herein represents a simple and enzyme-free detection. © 2013 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.bmcl.2013.10.005

Synthesis of novel 4′-C-methyl-1′,3′-dioxolane pyrimidine nucleosides and evaluation of its anti-HIV-1 activity 査読有り

Yutaka Kubota, Yuri Kaneda, Kazuhiro Haraguchi, Mirei Mizuno, Hiroshi Abe, Satoshi Shuto, Takayuki Hamasaki, Masanori Baba, Hiromichi Tanaka

Tetrahedron 69 巻 ( 51 ) 頁： 10884 - 10892 2013年12月

An epidermal growth factor derivative with binding affinity for hydroxyapatite and titanium surfaces 査読有り

Kang Jeonghwa, Tada Seiichi, Sakuragi Makoto, Abe Hiroshi, Ito Reiko, Ishikawa Junko, Kurata Shino, Kitajima Takashi, Son Tae Il, Aigaki Toshiro, Ito Yoshihiro

BIOMATERIALS 34 巻 ( 38 ) 頁： 9747 - 9753 2013年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Biomaterials

An epidermal growth factor (EGF) derivative with affinity for apatite and titanium surfaces was designed using a peptide moiety derived from salivary statherin, a protein that adheres to hydroxyapatite. Since the active sequence has two phosphoserine residues, the EGF derivative was prepared by organic synthesis, and a 54 residue peptide was successfully prepared using this method. Circular dichroism spectra indicated that the conformation of EGF was not significantly altered by the addition of the affinity peptide sequence and the mitogenic activity was only slightly reduced when compared with the wild-type protein. However, the binding affinity of the modified EGF to hydroxyapatite and titanium was significantly higher than the unmodified EGF. The phosphate groups in the affinity sequence contributed to the affinity of modified EGF to both apatite and titanium. The modified EGF significantly enhanced the growth of cells on hydroxyapatite and titanium. It was also demonstrated that the bound EGF enhanced the signal transduction for longer periods than unbound EGF. In conclusion, the modified EGF had significantly higher binding affinity for apatite and titanium than soluble EGF, and the bound EGF significantly enhanced cell growth by long-lasting activation of intracellular signal transduction. © 2013 Elsevier Ltd.

DOI： 10.1016/j.biomaterials.2013.09.004

PEGylated antibodies and DNA in organic media and genetic PEGylation

Tada S., Abe H., Ito Y.

ACS Symposium Series 1144 巻頁： 223 - 233 2013年11月

出版者・発行元：ACS Symposium Series

Polyethylene glycol (PEG) is an amphiphilic polymer that is soluble in both water and organic media and can add many functions to biopolymers by conjugation. Here, PEG was coupled to antibodies or oligonucleotides for solubilization into organic media. The former interacted with antigens and the latter formed specific structures and had catalytic activity in organic media. In addition, we attempted to incorporate PEG genetically into a polypeptide. Although the PEG incorporation ratio decreased as the molecular weight of PEG was increased, PEG with a molecular weight of ≤1000 Da was incorporated successfully. The potential applications of PEGylated biopolymers and the methodology for gene PEGylation are discussed. © 2013 American Chemical Society.

DOI： 10.1021/bk-2013-1144.ch016

Scopus

Very Rapid DNA-Templated Reaction for Efficient Signal Amplification and Its Steady-State Kinetic Analysis of the Turnover Cycle 査読有り

Shibata Aya, Uzawa Takanori, Nakashima Yuko, Ito Mika, Nakano Yukiko, Shuto Satoshi, Ito Yoshihiro, Abe Hiroshi

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 135 巻 ( 38 ) 頁： 14172 - 14178 2013年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of the American Chemical Society

Oligonucleotide-templated reactions are powerful tools for the detection of nucleic acid sequences. One of the major scientific challenges associated with this technique is the rational design of non-enzyme-mediated catalytic templated reactions capable of multiple turnovers that provide high levels of signal amplification. Herein, we report the development of a nucleophilic aromatic substitution reaction-triggered fluorescent probe. The probe underwent a rapid templated reaction without any of the undesired background reactions. The fluorogenic reaction conducted in the presence of a template provided a 223-fold increase in fluorescence after 30 s compared with the nontemplated reaction. The probe provided an efficient level of signal amplification that ultimately enabled particularly sensitive levels of detection. Assuming a simple model for the templated reactions, it was possible to estimate the rate constants of the chemical reaction in the presence and in the absence of the template. From these kinetic analyses, it was possible to confirm that an efficient turnover cycle had been achieved, on the basis of the dramatic enhancement in the rate of the chemical reaction considered to be the rate-determining step. With maximized turnover efficiency, it was demonstrated that the probe could offer a high turnover number of 1500 times to enable sensitive levels of detection with a detection limit of 0.5 pM in the catalytic templated reactions. © 2013 American Chemical Society.

DOI： 10.1021/ja404743m

Long-Lived Luminogenic Probe for Detection of RNA in a Crude Solution of Living Bacterial Cells 査読有り

Saneyoshi Hisao, Ito Yoshihiro, Abe Hiroshi

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 135 巻 ( 37 ) 頁： 13632 - 13635 2013年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of the American Chemical Society

A pre-type sensitizer for a lanthanide complex on an oligonucleotide was successfully converted to a perfect final structure in a target DNA/RNA-templated reaction, without any chemical reagent or enzyme, under neutral conditions. The final form of the lanthanide-oligonucleotide provided a long-lived luminescence signal, appropriate for time-gated luminescence analysis and signal amplification. Target DNA/RNA-assisted time-gated luminescence analysis is a powerful tool for elimination of autofluorescence and detection of target RNA in living bacterial cells. © 2013 American Chemical Society.

DOI： 10.1021/ja406724k

Sustained delivery of siRNA from dopamine-coated stainless steel surfaces 査読有り

Joddar Binata, Albayrak Aydin, Kang Jeonghwa, Nishihara Mizuki, Abe Hiroshi, Ito Yoshihiro

ACTA BIOMATERIALIA 9 巻 ( 5 ) 頁： 6753 - 6761 2013年5月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Acta Biomaterialia

Dopamine, an adhesive protein can be covalently deposited onto biomaterials. In this study, we evaluated the ability of dopamine-coated surfaces for small interfering RNA (siRNA) immobilization and release. Dopamine was deposited onto 316L stainless steel discs either as a monolayer at acidic pH or as polydopamine at alkaline pH, following which siRNA was immobilized onto these discs. To investigate the RNA interference ability of immobilized siRNA, reduction of luciferase expression in HeLa, and reduction of Egr-1 expression and cell proliferation in human aortic smooth muscle cells (HAoSMCs) were determined. Dopamine treatment of 316L stainless steel discs under both the acidic and alkaline conditions resulted in the deposition of amino (NH 2) groups, which enabled electrostatic immobilization of siRNA. The immobilized siRNA was released from both types of coatings, and enhanced the percent suppression of firefly luciferase activity of HeLa significantly up to ∼96.5% compared to HeLa on non-dopamine controls (18%). Both the release of siRNA and the percent suppression of firefly luciferase activity were sustained for at least 7 days. In another set of experiments, siRNA sequences targeting to inhibit the activity of the transcription factor Egr-1 were eluted from dopamine-coated surfaces to HAoSMCs. Egr-1 siRNA eluted from dopamine-coated surfaces, significantly reduced the proliferation of HAoSMCs and their protein expression of Egr-1. Therefore, this method of surface immobilization of siRNA onto dopamine-coated surfaces might be effective for nucleic acid delivery from stents. © 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.actbio.2013.01.007

Nano-sized Molecule: New Approach for Diagnosis and Drug Discovery Foreword 査読有り

Abe Hiroshi, Mizukami Shin

YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN 133 巻 ( 3 ) 頁： 349 - 349 2013年3月

Nanostructured RNA for RNA Intereference 査読有り

Abe Hiroshi

YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN 133 巻 ( 3 ) 頁： 373 - 378 2013年3月

記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：Yakugaku Zasshi

RNA interference (RNAi) is a potent and highly specific gene-silencing phenomenon which is initiated or triggered by double-stranded RNAs (dsRNAs). Shortly after the development of RNAi, small interfering RNAs (siRNAs) that are 21 nucleotides in length with a 3′ nucleotide overhang were shown to be very effective in mammalian cells. Much effort has been dedicated to the application of siRNAs, both as biological tools and as therapeutic agents. Currently, synthetic siRNA would be the method of choice for clinical purposes. However, natural RNA strands are quickly degraded in biological fluids. Chemically synthesized unnatural nucleotides have been developed and introduced into the siRNA strand. For example, modification of the ribose moiety with a 2′-deoxy, 2′-O-methyl, or 2′-fluoro group, or modification of the phosphate backbone have been examined. Although these modifications improve the stability of siRNA in serum, they often cause a decrease in RNAi activity. There is also concern that unnatural RNA derivatives are toxic in the human body. A method to stabilize nontoxic natural RNA strands should be very useful for applications in RNAi technology. We came up with an idea that nano-structural design stabilizes natural RNA. We tested several new designs such as dumbbell RNA, double stranded circular RNA, or branched RNA in biological stability and RNA interference activity. Consequently, dumbbell or branched design offered prolonged RNAi effect due to high biological stability. © 2013 The Pharmaceutical Society of Japan.

DOI： 10.1248/yakushi.12-00239-4

Synthesis of a nucleoside phosphorodithioate analogue responsive to microenvironmental changes through chiral induction 査読有り

Saneyoshi Hisao, Mashimo Takushi, Hatano Ken, Ito Yoshihiro, Abe Hiroshi

TETRAHEDRON LETTERS 54 巻 ( 9 ) 頁： 1080 - 1083 2013年2月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Tetrahedron Letters

We have synthesized a 2′-aminomethyl branched-chain sugar nucleoside phosphorodithioate from 2,2′-anhydro uridine and subjected the material to a subsequent cyclization reaction under aqueous conditions using bi-functional linkers. The rate of the cyclization reaction was dependent on the leaving group on the bi-functional linkers. The generation of a chiral phosphorous peak from the achiral precursor, as indicated by 31P NMR, was identified as a good indicator for potentially probing the local and global features of the DNA structure. © 2012 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.tetlet.2012.12.028

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PEGylated Antibodies and DNA in Organic Media and Genetic PEGylation 査読有り

Seiichi Tada, Hiroshi Abe, Yoshihiro Ito

GREEN POLYMER CHEMISTRY: BIOCATALYSIS AND MATERIALS II 1144 巻頁： 223 - 233 2013年

記述言語：英語掲載種別：研究論文（国際会議プロシーディングス）出版者・発行元：AMER CHEMICAL SOC

Polyethylene glycol (PEG) is an amphiphilic polymer that is soluble in both water and organic media and can add many functions to biopolymers by conjugation. Here, PEG was coupled to antibodies or oligonucleotides for solubilization into organic media. The former interacted with antigens and the latter formed specific structures and had catalytic activity in organic media. In addition, we attempted to incorporate PEG genetically into a polypeptide. Although the PEG incorporation ratio decreased as the molecular weight of PEG was increased, PEG with a molecular weight of <= 1000 Da was incorporated successfully. The potential applications of PEGylated biopolymers and the methodology for gene PEGylation are discussed.

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