研究者詳細 - 阿部　洋

論文 - 阿部　洋

分割表示 248 件中 121 - 140 件目／全件表示 >>

Fluorogenic probes using 4-substituted-2-nitrobenzenesulfonyl derivatives as caging groups for the analysis of human glutathione transferase catalyzed reactions 査読有り

Shibata Aya, Nakano Yukiko, Ito Mika, Araki Mika, Zhang Jie, Yoshida Yasuhiko, Shuto Satoshi, Mannervik Bengt, Mogenstern Ralf, Ito Yoshihiro, Abe Hiroshi

ANALYST 138 巻 ( 24 ) 頁： 7326 - 7330 2013年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Analyst

We have synthesized a series of 4-substituted-2-nitrobenzene-sulfonyl compounds for caged fluorogenic probes and conducted a Hammett plot analysis using the steady-state kinetic parameters. The results revealed that the glutathione transferase (GST) alpha catalyzed reaction was dependent on the σ value in the same way as the non-enzymatic reaction, whereas the dependence of the σ value of the GST mu and pi was not as pronounced as that of GST alpha. © 2013 The Royal Society of Chemistry.

DOI： 10.1039/c3an01339a

RNA-templated molecule release induced protein expression in bacterial cells 査読有り

Shibata Aya, Ito Yoshihiro, Abe Hiroshi

CHEMICAL COMMUNICATIONS 49 巻 ( 3 ) 頁： 270 - 272 2013年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Chemical Communications

We have developed a system to release a biologically active molecule in response to the sequence of a target gene. The releasing system, which was triggered by the reduction of an azidomethyl group, was successfully applied to protein expression induced by the release of IPTG triggered by endogenous RNA in bacterial cells. © 2012 The Royal Society of Chemistry.

DOI： 10.1039/c2cc37826d

One-Pot Ring-Closing Metathesis/1,3-Dipolar Cycloaddition through Assisted Tandem Ruthenium Catalysis: Synthesis of a Dye with Isoindolo[2,1-a]quinoline Structure 査読有り

Arisawa Mitsuhiro, Fujii Yuki, Kato Hiroshige, Fukuda Hayato, Matsumoto Takashi, Ito Mika, Abe Hiroshi, Ito Yoshihiro, Shuto Satoshi

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 52 巻 ( 3 ) 頁： 1003 - 1007 2013年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Angewandte Chemie - International Edition

The one-pot tandem reaction of N-alkyl-N-allyl-2-vinylaniline derivatives with benzo- or naphthoquinones and a ruthenium-alkylidene catalyst leads to isoindolo[2,1-a]quinolines in a variety of colors, which can be altered by exchanging the substituent on the core heterocycle (see scheme). This reaction offers a new synthetic method for π-conjugated small molecules from simple aniline derivatives. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

DOI： 10.1002/anie.201206765

Cationic cholesterol-modified gelatin as an in vitro siRNA delivery vehicle 査読有り

Kadengodlu Pallavi Ananda, Aigaki Toshiro, Abe Hiroshi, Ito Yoshihiro

MOLECULAR BIOSYSTEMS 9 巻 ( 5 ) 頁： 965 - 968 2013年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Molecular BioSystems

Polymeric micelle prepared through the self-assembly of cationic cholesterol-modified gelatin was tested for siRNA delivery. It exerted the desired effect of gene knockdown in HeLa cells stably expressing the luciferase gene and achieved a two-fold increase in the knockdown ability when compared to Lipofectamine® 2000. It was found that the polymeric micelle exhibited excellent stability and increased the biological stability of the siRNA in serum. © 2013 The Royal Society of Chemistry.

DOI： 10.1039/c2mb25424g

Rolling Circle Amplification in a Prokaryotic Translation System Using Small Circular RNA 査読有り

Abe Naoko, Hiroshima Michio, Maruyama Hideto, Nakashima Yuko, Nakano Yukiko, Matsuda Akira, Sako Yasushi, Ito Yoshihiro, Abe Hiroshi

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 52 巻 ( 27 ) 頁： 7004 - 7008 2013年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Angewandte Chemie - International Edition

Getting the runaround: Small circular RNA molecules containing an infinite open reading frame were synthesized and tested in an E. coli cell-free translation system. A circular RNA 126 nucleotides in length was found to produce more product than its linear counterpart by two orders of magnitude, because a ribosome can work more effectively towards the elongation on circular RNA than it can on linear RNA in this continuous peptide synthesis. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

DOI： 10.1002/anie.201302044

ナノ構造化RNAを用いたRNA干渉法

阿部洋

薬学雑誌 133 巻 ( 3 ) 頁： 373-378 2013年

"PEGylated Antibodies and DNA in Organic Media and Genetic PEGylation" in "Green Polymer 2"

Seiichi Tada, Horoshi Abe, and Yoshihiro Ito

American Chemical Society 頁： 223-233 2013年

PEGylated Antibodies and DNA in Organic Media and Genetic PEGylation

Tada Seiichi, Abe Hiroshi, Ito Yoshihiro

GREEN POLYMER CHEMISTRY: BIOCATALYSIS AND MATERIALS II 1144 巻頁： 223 - 233 2013年

Peroxidase Activity of G-Quadruplex Hemin-Binding DNA Aptamers Determined by Electrochemical Measurement

Kubo I., Eguchi T., Hoshino Y., Liu M., Abe H., Ito T.

BIOENGINEERING BASED ON ELECTROCHEMISTRY 50 巻 ( 28 ) 頁： 1 - 7 2013年

出版者・発行元：ECS Transactions

Certain guanine-rich DNA oligomers that form a G-quadruplex structure and bind hemin inside are called hemin-binding DNA aptamers. A hemin-binding DNA aptamer with the 4c15 sequence forms a parallel G quadraplex and the PS2.M sequence folds and forms an anti-parallel structure. We propose a novel method to examine the peroxidase activity of hemin-binding DNA aptamers through electrochemical measurements. In this study, a polyA chain and thiol were introduced to the 5' terminal of each oligonucleotide. The peroxidase activity of hemin-binding DNA aptamers was determined after modifying a gold electrode with the aptamers. The distance to hemin from the surface of the electrode is most important to determine catalytic activity. Catalytic activity, shown as KM of the examined parallel DNA aptamers, was around 20 μM. 4c15 without a poly A linker showed the highest catalytic activity, and its KM was 19 μM. The KM of the anti-parallel aptamer was almost the same as that of the parallel aptamer. © The Electrochemical Society.

DOI： 10.1149/05028.0001ecst

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Immobilization of epidermal growth factor on titanium and stainless steel surfaces via dopamine treatment 査読有り

Kang Jeonghwa, Sakuragi Makoto, Shibata Aya, Abe Hiroshi, Kitajima Takashi, Tada Seiichi, Mizutani Masayoshi, Ohmori Hitoshi, Ayame Hirohito, Son Tae Il, Aigaki Toshiro, Ito Yoshihiro

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 32 巻 ( 8 ) 頁： 2552 - 2561 2012年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Materials Science and Engineering C

Titanium and stainless steel were modified with dopamine for the immobilization of biomolecules, epidermal growth factor (EGF). First, the treatment of metal surfaces with a dopamine solution under different pH conditions was investigated. At higher pH, the dopamine solution turned brown and formed precipitates. Treatment of the metals with dopamine at pH 8.5 also resulted in the development of brown color at the surface of the metals. The hydrophobicity of the surfaces increased after treatment with dopamine, independently of pH. X-ray photoelectron spectroscopy revealed the formation of a significant amount of an organic layer on both surfaces at pH 8.5. According to ellipsometry measurements, the organic layer formed at pH 8.5 was about 1000 times as thick as that formed at pH 4.5. The amount of amino groups in the layer formed at pH 8.5 was also higher than that observed in the layer formed at pH 4.5. EGF molecules were immobilized onto the dopamine-treated surfaces via a coupling reaction using carbodiimide. A greater amount of EGF was immobilized on surfaces treated at pH 8.5 compared with pH 4.5. Significantly higher growth of rat fibroblast cells was observed on the two EGF-immobilized surfaces compared with non-immobilized surfaces in the presence of EGF. The present study demonstrated that metals can become bioactive via the surface immobilization of a growth factor and that the effect of the immobilized growth factor on metals was greater than that of soluble growth factor. © 2012 Elsevier B.V.

DOI： 10.1016/j.msec.2012.07.039

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Detection of pre-mRNA splicing in vitro by an RNA-templated fluorogenic reaction 査読有り

Tamura Yasutsugu, Furukawa Kazuhiro, Yoshimoto Rei, Kawai Yuto, Yoshida Minoru, Tsuneda Satoshi, Ito Yoshihiro, Abe Hiroshi

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 22 巻 ( 23 ) 頁： 7248 - 7251 2012年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Bioorganic and Medicinal Chemistry Letters

RNA splicing is an important target for basic research of disease mechanisms and for drug discovery. Here, we report a new method for analysis of the in vitro RNA splicing process that produces fluorescence using a reduction-triggered fluorescence (RETF) probe. The fluorescence signal is produced only when the two probes bind side-by-side with a specific RNA target. Precursor messenger RNA and mature messenger RNA originating from the chicken δ-crystallin (CDC) gene were successfully discriminated in solution using an RETF probe with the assistance of helper oligonucleotide strands. Also, we successfully applied RETF probes to the detection of emerging mature mRNA in an in vitro splicing process. © 2012 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.bmcl.2012.09.033

ナノ構造を制御したRNA干渉法

阿部洋、阿部奈保子、柴田綾、伊藤嘉浩

アンチセンスDNA/RNA研究会誌 16 巻 ( 2 ) 頁： 3 2012年11月

Design and Synthesis of Indomethacin Analogues That Inhibit P-Glycoprotein and/or Multidrug Resistant Protein without Cox Inhibitory Activity 査読有り

Arisawa Mitsuhiro, Kasaya Yayoi, Obata Tohru, Sasaki Takuma, Nakamura Tomonori, Araki Takuya, Yamamoto Koujirou, Sasaki Akito, Yamano Akihito, Ito Mika, Abe Hiroshi, Ito Yoshihiro, Shuto Satoshi

JOURNAL OF MEDICINAL CHEMISTRY 55 巻 ( 18 ) 頁： 8152 - 8163 2012年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of Medicinal Chemistry

We designed and synthesized conformationally restricted analogues and regioisomers of the nonsteroidal anti-inflammatory drug indomethacin. Evaluation of the inhibitory effects of these compounds on COX, P-glycoprotein, and multidrug resistance indicated that NSAIDS modulation of multidrug-resistant P-glycoprotein and multidrug-resistant protein-1 is not associated with COX-1 and COX-2 inhibitory activities. © 2012 American Chemical Society.

DOI： 10.1021/jm301084z

Development of new luminogenic probes for detection of glutathione s-transferase activity in living cells

Ito Mika, Shibata Aya, Abe Hiroshi, Zhang Jie, Morgenstern Ralf, Shuto Satoshi, Ito Yoshihiro

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 244 巻 2012年8月

Development of new luminogenic probes for detection of glutathione s-transferase activity in living cells 査読有り

Mika Ito, Aya Shibata, Hiroshi Abe, Jie Zhang, Ralf Morgenstern, Satoshi Shuto, Yoshihiro Ito

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 244 巻 2012年8月

Universal Caging Group for the in-Cell Detection of Glutathione Transferase Applied to 19F NMR and Bioluminogenic Probes 査読有り

Ito Mika, Shibata Aya, Zhang Jie, Hiroshima Michio, Sako Yasushi, Nakano Yukiko, Kojima-Aikawa Kyoko, Mannervik Bengt, Shuto Satoshi, Ito Yoshihiro, Morgenstern Ralf, Abe Hiroshi

CHEMBIOCHEM 13 巻 ( 10 ) 頁： 1428 - 1432 2012年7月

還元反応を引き金とする off/on 型蛍光プローブを用いたRNA検出法の開発

阿部洋, 柴田綾, 古川和寛, 常田聡, 伊藤嘉浩

化学と生物 50 巻 ( 7 ) 頁： 540 - 544 2012年7月

環元反応を引き金とするoff/on 型蛍光プローブを用いたRNA検出法の開発

阿部洋、柴田綾、古川和寛、常田聡、伊藤嘉浩

化学と生物 50 巻 ( 7月 ) 頁： 540 2012年7月

Oligonucleotide-Templated Reactions for Sensing Nucleic Acids 査読有り

Shibata Aya, Abe Hiroshi, Ito Yoshihiro

MOLECULES 17 巻 ( 3 ) 頁： 2446 - 2463 2012年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Molecules

Oligonucleotide-templated reactions are useful for applying nucleic acid sensing. Various chemistries for oligonucleotide-templated reaction have been reported so far. Major scientific interests are focused on the development of signal amplification systems and signal generation systems. We introduce the recent advances of oligonucleotide-templated reaction in consideration of the above two points. © 2012 by the authors.

DOI： 10.3390/molecules17032446

Synthesis of Dumbbell-Shaped Cyclic RNAs for RNA Interference

Naoko Abe, Hiroshi Abe, Yoshihiro Ito

Current Protocol Nucleic Acid Chemistry 頁： 1-16 2012年

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