研究者詳細 - 吉村　崇

論文 - 吉村　崇

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Deep brain photoreceptors and a seasonal signal transduction cascade in birds

Nakane Yusuke, Yoshimura Takashi

CELL AND TISSUE RESEARCH 342 巻 ( 3 ) 頁： 341 - 344 2010年12月

A mammalian neural tissue opsin (Opsin 5) is a deep brain photoreceptor in birds

Nakane Yusuke, Ikegami Keisuke, Ono Hiroko, Yamamoto Naoyuki, Yoshida Shosei, Hirunagi Kanjun, Ebihara Shizufumi, Kubo Yoshihiro, Yoshimura Takashi

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 107 巻 ( 34 ) 頁： 15264 - 15268 2010年8月

Usp46, encoding a ubiquitin specific peptidase, is a quantitative trait gene underlying "behavioral despair" in mice 査読有り

Ebihara Shizufumi, Tomida Shigeru, Mamiya Takayoshi, Sakamaki Hirotake, Miura Masami, Aosaki Toshihiko, Masuda Masao, Niwa Minae, Kameyama Tsutomu, Kobayashi Junya, Iwaki Yuka, Imai Saki, Ishikawa Akira, Abe Kuniya, Yoshimura Takashi, Nabeshima Toshitaka

SLEEP AND BIOLOGICAL RHYTHMS 8 巻 ( 2 ) 頁： 114 - 119 2010年4月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Sleep and Biological Rhythms

CS mice exhibit several distinct phenotypes of circadian behavioral rhythms and sleep properties. Because many mental illnesses are associated with abnormalities in the circadian rhythms and sleep pattern, we characterized the behavioral phenotypes in CS mice with a battery of behavioral tests. Among these phenotypes, we found that CS mice exhibit an extremely low immobility time in both the tail suspension test (TST) and forced swimming test (FST). To uncover the genetic basis for lower immobility time, we first performed quantitative trait locus (QTL) mapping using CS and C57BL/6J mice, which revealed significant QTLs on chromosomes (Chrs) 4 (FST) and 5 (TST and FST). To identify the quantitative trait gene on Chr 5, we narrowed the QTL interval to 0.5 Mb using several congenic and subcongenic strains. Ubiquitin-specific peptidase 46 (Usp46) with a lysine codon deletion was located in this region. This deletion affected nest-building, muscimol-induced righting reflex, and anti-immobility effects of imipramine. The muscimol-induced current in the hippocampal CA1 pyramidal neurons and hippocampal expression of the 67-kDa isoform of glutamic acid decarboxylase significantly decreased in Usp46 mutant mice. All these phenotypes were rescued in transgenic mice with bacterial artificial chromosomes containing wild-type Usp46. Thus, Usp46 affects the immobility in the TST and FST, and it is implicated in the regulation of GABA action. © 2010 The Authors. Journal compilation © 2010 Japanese Society of Sleep Research.

DOI： 10.1111/j.1479-8425.2010.00435.x

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Influence of the estrous cycle on clock gene expression in reproductive tissues: Effects of fluctuating ovarian steroid hormone levels 査読有り

Nakamura Takahiro J., Sellix Michael T., Kudo Takashi, Nakao Nobuhiro, Yoshimura Takashi, Ebihara Shizufumi, Colwell Christopher S., Block Gene D.

STEROIDS 75 巻 ( 3 ) 頁： 203 - 212 2010年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Steroids

Circadian rhythms in physiology and behavior are known to be influenced by the estrous cycle in female rodents. The clock genes responsible for the generation of circadian oscillations are widely expressed both within the central nervous system and peripheral tissues, including those that comprise the reproductive system. To address whether the estrous cycle affects rhythms of clock gene expression in peripheral tissues, we first examined rhythms of clock gene expression (Per1, Per2, Bmal1) in reproductive (uterus, ovary) and non-reproductive (liver) tissues of cycling rats using quantitative real-time PCR (in vivo) and luminescent recording methods to measure circadian rhythms of PER2 expression in tissue explant cultures from cycling PER2::LUCIFERASE (PER2::LUC) knockin mice (ex vivo). We found significant estrous variations of clock gene expression in all three tissues in vivo, and in the uterus ex vivo. We also found that exogenous application of estrogen and progesterone altered rhythms of PER2::LUC expression in the uterus. In addition, we measured the effects of ovarian steroids on clock gene expression in a human breast cancer cell line (MCF-7 cells) as a model for endocrine cells that contain both the steroid hormone receptors and clock genes. We found that progesterone, but not estrogen, acutely up-regulated Per1, Per2, and Bmal1 expression in MCF-7 cells. Together, our findings demonstrate that the timing of the circadian clock in reproductive tissues is influenced by the estrous cycle and suggest that fluctuating steroid hormone levels may be responsible, in part, through direct effects on the timing of clock gene expression. © 2010 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.steroids.2010.01.007

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Zona pellucida protein ZP2 is expressed in the oocyte of Japanese quail (Coturnix japonica)

Kinoshita Mihoko, Rodler Daniela, Sugiura Kenichi, Matsushima Kayoko, Kansaku Norio, Tahara Kenichi, Tsukada Akira, Ono Hiroko, Yoshimura Takashi, Yoshizaki Norio, Tanaka Ryota, Kohsaka Tetsuya, Sasanami Tomohiro

REPRODUCTION 139 巻 ( 2 ) 頁： 359 - 371 2010年2月

出版者・発行元：Reproduction

The avian perivitelline layer (PL), a vestment homologous to the zona pellucida (ZP) of mammalian oocytes, is composed of at least three glycoproteins. Our previous studies have demonstrated that the matrix's components, ZP3 and ZPD, are synthesized in ovarian granulosa cells. Another component, ZP1, is synthesized in the liver and is transported to the ovary by blood circulation. In this study, we report the isolation of cDNA encoding quail ZP2 and its expression in the female bird. By RNase protection assay and in situ hybridization, we demonstrate that ZP2 transcripts are restricted to the oocytes of small white follicles (SWF). The expression level of ZP2 decreased dramatically during follicular development, and the highest expression was observed in the SWF.Western blot and immunohistochemical analyses using the specific antibody against ZP2 indicate that the 80 kDa protein is the authentic ZP2, and the immunoreactive ZP2 protein is also present in the oocytes. Moreover, ultrastructural analysis demonstrated that the immunoreactive ZP2 localizes to the zona radiata, the perivitelline space, and the oocyte cytoplasm in the SWF. By means of western blot analysis and immunofluorescence microscopy, we detected a possible interaction of the recombinant ZP2 with ZP3 and that this interaction might lead to the formation of amorphous structure on the cell surface. These results demonstrate for the first time that the avian ZP gene is expressed in the oocyte, and that the ZP2 protein in the oocyte might play a role for the PL formation in the immature follicles of the ovary. © 2010 Society for Reproduction and Fertility.

DOI： 10.1530/REP-09-0222

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Photoperiodic Control of TSH-β Expression in the Mammalian Pars Tuberalis has Different Impacts on the Induction and Suppression of the Hypothalamo-Hypopysial Gonadal Axis

Yasuo S., Yoshimura T., Ebihara S., Korf H. -W.

JOURNAL OF NEUROENDOCRINOLOGY 22 巻 ( 1 ) 頁： 43 - 50 2010年1月

出版者・発行元：Journal of Neuroendocrinology

Seasonal reproduction depends on photoperiod-regulated activation or suppression of the gonadal axis. Recent studies in quail have identified long-day induced . TSH-β expression in the pars tuberalis (PT) as a rapid trigger of gonadal activation. Thyroid-stimulating hormone (TSH) induces type 2 deiodinase (. Dio2) in the ependymal cell layer (EC) of the infundibular recess to stimulate the gonadal axis. A similar mechanism is proposed in sheep and mice, but the experimental data on the temporal patterns of induction and suppression of . TSH-β and . Dio2 expression are incomplete. In the present study, we examined the expression of . TSH-β and . Dio2 in hamsters transferred from short- to long-day conditions for 9 days, and demonstrate the induction of . TSH-β and . Dio2 on day 8 after transition. These data demonstrate the close relationship between . TSH-β and . Dio2 expression in the inductive pathway. The temporal expression of . TSH-β and . Dio2 in the suppressive pathway was also examined by s.c. melatonin injection, which mimics the transition from long to short days. Importantly, . Dio2 expression in the EC is suppressed on day 1 after the onset of injection, whereas . TSH-β expression in the PT was not suppressed until day 10. These data suggest that regulated transcription of . TSH-β is involved in the induction of the gonadal axis in mammals, whereas the suppression of this axis is mediated by different mechanisms. © 2009 The Authors. Journal Compilation © 2009 Blackwell Publishing Ltd.

DOI： 10.1111/j.1365-2826.2009.01936.x

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Neuroendocrine mechanism of seasonal reproduction in birds and mammals 招待有り査読有り

Yoshimura T

Animal Science Journal 81 巻頁： 302-410 2010年

動物が季節を感じるしくみをさぐる招待有り査読有り

吉村崇

日本の科学者 45 巻頁： 54-57 2010年

Deep brain photoreceptors and a seasonal signal transduction cascade in birds 招待有り査読有り

Nakane Y, Yoshimura T

Cell and Tissue Research in press 巻 2010年

脊椎動物の季節繁殖の制御機構招待有り

池上啓介、吉村崇

日本生殖内分泌学会雑誌 15 巻頁： 55-57 2010年

哺乳類の光周性におけるメラトニンの作用機構招待有り

星野佑太、小野ひろ子、吉村崇

比較内分泌学 36 巻頁： 96-101 2010年

Thyrotropin receptor (TSHR) null mice show methylphenidate-responsive ADHD-like behavioral alternations

Narusawa Shiho, Hoshino Yuta, Mouri Akihiro, Yoshimura Takashi, Nabeshima Toshitaka

JOURNAL OF PHARMACOLOGICAL SCIENCES 112 巻頁： 229P - 229P 2010年

Neuroendocrine mechanism of seasonal reproduction in birds and mammals

Yoshimura Takashi

ANIMAL SCIENCE JOURNAL 81 巻 ( 4 ) 頁： 403 - 410 2010年

記述言語：英語出版者・発行元：Animal Science Journal

In temperate zones, animals use changes in day length as a calendar to time their breeding season. However, the photoreceptive and neuroendocrine mechanisms of seasonal reproduction are considered to differ markedly between birds and mammals. This can be understood from the fact that the eye is the only photoreceptive organ, and melatonin mediates the photoperiodic information in mammals, whereas in birds, photoperiodic information is directly received by the deep brain photoreceptors and melatonin is not involved in seasonal reproduction. Recent molecular and functional genomics analysis uncovered the gene cascade regulating seasonal reproduction in birds and mammals. Long day-induced thyroid stimulating hormone in the pars tuberalis of the pituitary gland regulates thyroid hormone catabolism within the mediobasal hypothalamus. Further, this local thyroid hormone catabolism appears to regulate seasonal gonadotropin-releasing hormone secretion. These findings suggest that although the light input pathway is different between birds and mammals (i.e. light or melatonin), the core mechanisms are conserved in these vertebrates. © 2010 The Author; Journal compilation © 2010 Japanese Society of Animal Science.

DOI： 10.1111/j.1740-0929.2010.00777.x

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Localization of Circadian Clock Protein BMAL1 in the Photoperiodic Signal Transduction Machinery in Japanese Quail 査読有り国際誌

Ikegami Keisuke, Katou Yasuhiro, Higashi Kumiko, Yoshimura Takashi

JOURNAL OF COMPARATIVE NEUROLOGY 517 巻 ( 3 ) 頁： 397 - 404 2009年11月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of Comparative Neurology

The circadian clock is a fundamental property of living organisms and is involved in seasonal (photoperiodic) time measurement. Among vertebrates, birds have multiple circadian pacemakers in the eye, the pineal gland, and the suprachiasmatic nucleus (SCN), and have highly sophisticated photoperiodic mechanisms. However, because the removal of these circadian pacemakers fails to abolish the photoperiodic response, the existence of another "photoperiodic clock" has been suggested. Recent studies have revealed that the mediobasal hypothalamus (MBH) and the adjacent pars tuberalis (PT) of the pituitary gland constitute key components of the photoperiodic signal transduction machinery. In the present study, we generated a polyclonal antibody against the chicken circadian clock protein BMAL1 to examine BMAL1 distribution in the Japanese quail brain by using immunohistochemistry. BMAL1-like immunoreactivity (lir) was confirmed in the pineal gland and the medial SCN, which are critical circadian pacemakers. We also observed strong immunoreactivity in the MBH, including the ependymal cells (ECs), the infundibular nucleus (IN), the median eminence (ME), and the adjacent PT. Furthermore, semiquantitative analysis suggested that BMAL1-lir shows daily fluctuation in these regions. It is possible that circadian clocks in the photoperiodic signal transduction machinery such as the PT and the EC may be involved in the regulation of photoperiodism. © 2009 Wiley-Liss, Inc.

DOI： 10.1002/cne.22165

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Comparative analysis of the molecular basis of photoperiodic signal transduction in vertebrates

Yasuo Shinobu, Yoshimura Takashi

INTEGRATIVE AND COMPARATIVE BIOLOGY 49 巻 ( 5 ) 頁： 507 - 518 2009年11月

出版者・発行元：Integrative and Comparative Biology

In temperate zones, the reproductive physiology of most vertebrates is controlled by changes in photoperiod. Mechanisms for the regulation of photoperiodic gonadal responses are known to differ between mammals and birds: in mammals, melatonin is the photoperiodic signal messenger, whereas in birds, photoperiodic information is received by deep brain photoreceptors. Recently, the molecular mechanism of photoperiodism has been revealed by studies on Japanese quail, which exhibit a most remarkable responsiveness to photoperiod among vertebrates, and molecular cascades involved in photoperiodism have been elucidated. Long-day stimulus induces expression of the β-subunit of thyroid stimulating hormone (TSH-β) in the pars tuberalis (PT) of the pituitary gland, and TSH derived from the PT regulates reciprocal switching of genes encoding types 2 and 3 deiodinases (Dio2 and Dio3, respectively) in the mediobasal hypothalamus (MBH) by retrograde action. Dio2 locally converts prohormone thyroxine (T4) to bioactive triiodothyronine (T3) in the MBH, which subsequently stimulates the gonadal axis. These events have been confirmed to occur in mammals with seasonal breeding, such as hamsters and sheep, suggesting that similar mechanisms are involved among various vertebrates. In addition, nonphotoperiodic mice also appeared to possess the same molecular mechanisms at the hypothalamo-hypophysial level. It has been noted that melatonin regulates the above-mentioned key genes (Dio2, Dio3, and TSH-β) in mammals, while photoperiod directly regulates these genes in birds. Thus, the input pathway of photoperiod is different between mammals and birds (i.e., melatonin versus light); however, the essential mechanisms are conserved among these vertebrates.

DOI： 10.1093/icb/icp011

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Usp46 is a quantitative trait gene regulating mouse immobile behavior in the tail suspension and forced swimming tests

Tomida Shigeru, Mamiya Takayoshi, Sakamaki Hirotake, Miura Masami, Aosaki Toshihiko, Masuda Masao, Niwa Minae, Kameyama Tsutomu, Kobayashi Junya, Iwaki Yuka, Imai Saki, Ishikawa Akira, Abe Kuniya, Yoshimura Takashi, Nabeshima Toshitaka, Ebihara Shizufumi

NATURE GENETICS 41 巻 ( 6 ) 頁： 688 - 695 2009年6月

Red jungle fowl (Gallus gallus) as a model for studying the molecular mechanism of seasonal reproduction 査読有り

Ono Hiroko, Nakao Nobuhiro, Yamamura Takashi, Kinoshita Keiji, Mizutani Makoto, Namikawa Takao, Iigo Masayuki, Ebihara Shizufumi, Yoshimura Takashi

ANIMAL SCIENCE JOURNAL 80 巻 ( 3 ) 頁： 328 - 332 2009年6月

記述言語：英語出版者・発行元：Animal Science Journal

Photoperiodism is an adaptation mechanism that enables animals to predict seasonal changes in the environment. Japanese quail is the best model organism for studying photoperiodism. Although the recent availability of chicken genome sequences has permitted the expansion from single gene to genome-wide transcriptional analysis in this organism, the photoperiodic response of the domestic chicken is less robust than that of the quail. Therefore, in the present study, we examined the photoperiodic response of the red jungle fowl (Gallus gallus), a predecessor of the domestic chicken, to test whether this animal could be developed as an ideal model for studying the molecular mechanisms of seasonal reproduction. When red jungle fowls were transferred from short-day- to long-day conditions, gonadal development and an increase in plasma LH concentration were observed. Furthermore, rapid induction of thyrotropin beta subunit, a master regulator of photoperiodism, was observed at 16 h after dawn on the first long day. In addition, the long-day condition induced the expression of type 2 deiodinase, the key output gene of photoperiodism. These results were consistent with the results obtained in quail and suggest that the red jungle fowl could be an ideal model animal for the genome-wide transcriptional analysis of photoperiodism. © 2009 Japanese Society of Animal Science.

DOI： 10.1111/j.1740-0929.2009.00628.x

Melatonin Transmits Photoperiodic Signals through the MT1 Melatonin Receptor

Yasuo Shinobu, Yoshimura Takashi, Ebihara Shizufumi, Korf Horst-Werner

JOURNAL OF NEUROSCIENCE 29 巻 ( 9 ) 頁： 2885 - 2889 2009年3月

Molecular and Endocrine Mechanisms of Vertebrate Photoperiodic Response

Yoshimura T.

INTEGRATIVE AND COMPARATIVE BIOLOGY 49 巻頁： E187 - E187 2009年2月

Molecular characterization of egg envelope glycoprotein ZPD in the ovary of Japanese quail (Coturnix japonica)

Sato Tsukasa, Kinoshita Mihoko, Kansaku Norio, Tahara Kenichi, Tsukada Akira, Ono Hiroko, Yoshimura Takashi, Dohra Hideo, Sasanami Tomohiro

REPRODUCTION 137 巻 ( 2 ) 頁： 333 - 343 2009年2月

出版者・発行元：Reproduction

The egg envelope surrounding avian oocytes exhibits a three-dimensional network of coarse fibers between the granulosa cells and the oocyte. Our previous studies have demonstrated that one of the matrix's components, ZP3, is synthesized in the ovarian granulosa cells. Another component, ZP1, which is critically involved in triggering the sperm acrosome reaction, is synthesized in the liver. We have previously isolated cDNAs encoding quail ZP3 and ZP1, and we now report the isolation of cDNA encoding quail ZPD. By RNase protection assay and in situ hybridization, we have demonstrated that ZPD transcripts are restricted to the granulosa cells of preovulatory follicles. The expression level of ZPD increased progressively during follicular development, and the highest expression was observed in the largest follicles. Western blot analyses using the specific antibody against ZPD indicate that the 40 kDa protein is the authentic ZPD, and the contents of ZPD protein also increased during follicular development. Moreover, we found that the addition of FSH to the culture media enhances the ZPD secretion in the cultured granulosa cells. Two-dimensional gel electrophoresis revealed the presence of several ZPD isoforms with different pI values ranging from 5.5 to 7. Immunohistochemical analyses indicate that the materials recognized with anti-quail ZPD antibody were accumulated in the egg envelope of large yellow follicles. These results demonstrate the presence of ZPD protein in the egg envelope, and that the amount of ZPD in the egg envelope as well as the mRNA in the cells increases at the latter stages of folliculogenesis. © 2009 Society for Reproduction and Fertility.

DOI： 10.1530/REP-08-0057

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