研究者詳細 - 丸山　和昭

論文 - 丸山　和昭

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Organotypic brain explant culture as a drug evaluation system for malignant brain tumors

Minami, N; Maeda, Y; Shibao, S; Arima, Y; Ohka, F; Kondo, Y; Maruyama, K; Kusuhara, M; Sasayama, T; Kohmura, E; Saya, H; Sampetrean, O

CANCER MEDICINE 6 巻 ( 11 ) 頁： 2635 - 2645 2017年11月

記述言語：英語出版者・発行元：Cancer Medicine

Therapeutic options for malignant brain tumors are limited, with new drugs being continuously evaluated. Organotypic brain slice culture has been adopted for neuroscience studies as a system that preserves brain architecture, cellular function, and the vascular network. However, the suitability of brain explants for anticancer drug evaluation has been unclear. We here adopted a mouse model of malignant glioma based on expression of H-RasV12 in Ink4a/Arf−/− neural stem/progenitor cells to establish tumor-bearing brain explants from adult mice. We treated the slices with cisplatin, temozolomide, paclitaxel, or tranilast and investigated the minimal assays required to assess drug effects. Serial fluorescence-based tumor imaging was sufficient for evaluation of cisplatin, a drug with a pronounced cytotoxic action, whereas immunostaining of cleaved caspase 3 (a marker of apoptosis) and of Ki67 (a marker of cell proliferation) was necessary for the assessment of temozolomide action and immunostaining for phosphorylated histone H3 (a marker of mitosis) allowed visualization of paclitaxel-specific effects. Staining for cleaved caspase 3 was also informative in the assessment of drug toxicity for normal brain tissue. Incubation of explants with fluorescently labeled antibodies to CD31 allowed real-time imaging of the microvascular network and complemented time-lapse imaging of tumor cell invasion into surrounding tissue. Our results suggest that a combination of fluorescence imaging and immunohistological staining allows a unified assessment of the effects of various classes of drug on the survival, proliferation, and invasion of glioma cells, and that organotypic brain slice culture is therefore a useful tool for evaluation of antiglioma drugs.

DOI： 10.1002/cam4.1174

Web of Science

Scopus

PubMed

Molecular and clinical features of K<inf>ATP</inf>-channel neonatal diabetes mellitus in Japan

Hashimoto Y., Dateki S., Hirose M., Satomura K., Sawada H., Mizuno H., Sugihara S., Maruyama K., Urakami T., Sugawara H., Shirai K., Yorifuji T.

Pediatric Diabetes 18 巻 ( 7 ) 頁： 532 - 539 2017年11月

出版者・発行元：Pediatric Diabetes

Background: There are few reports pertaining to Asian patients with neonatal diabetes mellitus (NDM) caused by activating mutations in the ATP-sensitive potassium channel genes (KATP-NDM). Objectives: To elucidate the characteristics of Japanese patients with KATP-NDM. Methods: By the amplification and direct sequencing of all exons and exon-intron boundaries of the KCNJ11 and ABCC8 genes, 25 patients with KATP-NDM were identified from a total of 70 patients with NDM. Clinical data were collected from the medical charts. Results: Sixteen patients had mutations in KCNJ11 and nine in ABCC8. Eight novel mutations were identified; two in KCNJ11 (V64M, R201G) and six in ABCC8 (R216C, G832C, F1176L, A1263V, I196N, T229N). Interestingly, V64M caused DEND (developmental delay, epilepsy, neonatal diabetes) syndrome in our patient, while mutation of the same residue (V64G) had been reported to cause congenital hyperinsulinism. Mutations in ABCC8 were associated with TNDM (4/9) or isolated PNDM (5/9), whereas those in KCNJ11 were associated with more severe phenotypes, including DEND (3/16), iDEND (intermediate DEND, 4/16), or isolated PNDM (6/16). Switching from insulin to glibenclamide monotherapy was successful in 87.5% of the patients. Neurological improvement was observed in two patients, one with DEND (T293N) and one with iDEND (R50P) syndrome. Three others with iDEND mutations (R201C, G53D, and V59M) remained neurologically normal at 5, 1, and 4 years of age, respectively, with early introduction of sulfonylurea. Conclusion: Overall, clinical presentation of KATP-NDM in Japanese patients was similar to those of other populations. Early introduction of sulfonylurea appeared beneficial in ameliorating neurological symptoms.

DOI： 10.1111/pedi.12447

Scopus

Characteristics of epilepsy in patients with Kabuki syndrome with <i>KMT2D</i> mutations

Kurahashi, N; Miyake, N; Mizuno, S; Koshimizu, E; Kurahashi, H; Yamada, K; Natsume, J; Aoki, Y; Nakamura, M; Taniai, H; Maki, Y; Abe-Hatano, C; Matsumoto, N; Maruyama, K

BRAIN & DEVELOPMENT 39 巻 ( 8 ) 頁： 672 - 677 2017年9月

記述言語：英語出版者・発行元：Brain and Development

Background The characteristics of epilepsy in patients with Kabuki syndrome with KMT2D mutations (KABUK1) have not yet been well documented. This is the first review to explore this. Materials & methods We enrolled 14 patients with KABUK1, whose median age was 13.6 years (range = 4.1–21.3 years). Their medical records from October 1981 to May 2016 were retrospectively analyzed. Results Epilepsy was present in 5 (36%) patients. Four of these patients presented with nonsense mutations and one with missense mutations. None presented with brain abnormalities. Four patients presented with annual or monthly focal seizures, of which three evolved to bilateral convulsive seizures. Median onset age of focal epilepsy was 11.8 years (range = 9.5–12.8 years). One presented with monthly myoclonic seizures from age 11.2, whose mother with no other KABUK1 features, had focal epilepsy. The cumulative incidence of epilepsy related to KABUK1 up until age 13 was 45%. Interictal electroencephalogram revealed focal paroxysmal epileptiform discharges (in frontal, central, and parietal regions) in three patients, diffuse high-voltage spike-and-waves in one patient, and normal sleep record in one patient. Myoclonic seizures were rapidly controlled by levetiracetam. In contrast, focal seizures were not controlled in the early period of antiepileptic therapy. Conclusion This long-term follow-up of patients with KABUK1 revealed a higher prevalence of epilepsy than previously reported. The age of epilepsy onset and rate of focal seizures evolving to bilateral convulsive seizures in KABUK1 were also higher than previously reported in patients with clinically diagnosed Kabuki syndrome. Although seizure outcome is reported to be favorable in Kabuki syndrome, focal seizures in patients with KABUK1 were not immediately responsive to medication.

DOI： 10.1016/j.braindev.2017.03.025

Web of Science

Scopus

PubMed

Palivizumab prophylaxis in preterm infants and subsequent recurrent wheezing: Six-year follow-up study

Mochizuki H., Kusuda S., Okada K., Yoshihara S., Furuya H., Simões E.A.F., Asanuma H., Yoshida H., Katayose M., Imamura T., Suzumura H., Honma Y., Maruyama K., Ohki Y., Ozasa K., Nariai A., Uchiyama A., Uryu H., Hosono S., Kondo M., Kawase Y., Ikeda K., Naito A., Minami Y., Nakamura T., Baba A., Nagayama Y., Kaneda H., Ohki S., Shirai M., Henmi Y., Suzuki C., Ieda K., Tanaka T., Kono Y., Nisizawa K., Niwa F., Ito H., Tokunaga Y., Takada Y., Nishikawa Y., Nagata I., Kajino Y., Watabe S., Yoshio H., Takahashi N., Sasaki N., Hayashidani M., Tateishi H., Matsushita K., Nakamura M., Nakayama H., Yamamoto G., Kanemitsu N., Takayanagi T., Sato A., Ibara S.

American Journal of Respiratory and Critical Care Medicine 196 巻 ( 1 ) 頁： 29 - 38 2017年7月

出版者・発行元：American Journal of Respiratory and Critical Care Medicine

Rationale: Respiratory syncytial virus (RSV) induces not only infantile recurrent wheezing but also potentially atopic asthma. Objectives: To test the effect of RSV infection on development of subsequent atopic asthma, we evaluated whether palivizumab, an anti-RSV monoclonal antibody, by preventing severe RSV disease in the first year of life, could impact subsequent recurrent wheezing and atopic asthma at 6 years of age. Methods: During the 2007 to 2008 RSV season, the decision to administer palivizumab was made based on standard medical practice and an observational prospective multicenter (n = 52) case-control study in preterm infants with a gestational age between 33 and 35 weeks followed from 0 to 3 years (preceding Committee on Recurrent Wheezing study). The 52 investigators at hospitals then followed these subjects until 6 years of age, reported here (Effects of Preventive Treatment for Respiratory Syncytial [RS] Virus Infection During Infancy on Later Atopic Asthma in Preterm Infants; Scientific Committee for Elucidation of Infantile Asthma). Parents of study subjects reported the infants' physicians' assessment of recurrent wheezing, using a report card and a novel mobile phone-based reporting system using the Internet. The primary endpoint was the incidence of atopic asthma. Measurements and Main Results: Of 444 preterm infants enrolled, 349 received palivizumab during the first year of life. At 6 years, atopic asthma was not different in the groups: 15.3 and 18.2% of infants in the treated and untreated groups, respectively (P = 0.57). On the other hand, physician-diagnosed recurrent wheezing was observed in 15.3 and 31.6% in the treated and untreated groups, respectively (P = 0.003). Conclusions: Palivizumab prophylaxis administered to preterm infants did not suppress the onset of atopic asthma but resulted in a significantly lower incidence of recurrent wheezing during the first 6 years.

DOI： 10.1164/rccm.201609-1812OC

Scopus

Frame-based behavior preservation in refactoring

Maruyama K., Hayashi S., Yoshida N., Choi E.

SANER 2017 - 24th IEEE International Conference on Software Analysis, Evolution, and Reengineering 頁： 573 - 574 2017年3月

出版者・発行元：SANER 2017 - 24th IEEE International Conference on Software Analysis, Evolution, and Reengineering

Behavior preservation often bothers programmers in refactoring. This poster paper proposes a new approach that tames the behavior preservation by introducing the concept of a frame. A frame in refactoring defines stakeholder's individual concerns about the refactored code. Frame-based refactoring preserves the observable behavior within a particular frame. Therefore, it helps programmers distinguish the behavioral changes that they should observe from those that they can ignore.

DOI： 10.1109/SANER.2017.7884683

Scopus

Sleep duration, snoring prevalence, obesity, and behavioral problems in a large cohort of primary school students in Japan

Sakamoto N., Gozal D., Smith D.L., Yang L., Morimoto N., Wada H., Maruyama K., Ikeda A., Suzuki Y., Nakayama M., Horiguchi I., Tanigawa T.

Sleep 40 巻 ( 3 ) 2017年3月

出版者・発行元：Sleep

Study Objectives: Poor or short sleep and the presence of snoring indicative of sleep-disordered breathing (SDB) have been associated with behavioral problems in school-aged children. We examined the relationship between SDB, sleep duration, obesity risk, and behavioral characteristics in Japanese elementary school students using a large-scale survey. Methods: We conducted a cross-sectional study of children enrolled in all 46 public primary schools in Matsuyama city, Japan. The children's parents or guardians completed a questionnaire that covered sleep habits, presence of SDB risk, and behavioral characteristics. Results: In total, 24 296 responses were received (90% response rate). After excluding incomplete responses, we analyzed complete datasets for 17 769 children. Mean sleep duration decreased with age, as did the prevalence of pediatric SDB. We found an increased risk for the presence of SDB and short sleep among overweight/obese children. With SDB or short sleep, we observed significantly increased odds of restless behaviors, fidgety behaviors, and poor concentration in school. Conclusions: Shorter sleep duration was associated with increased risk of obesity, and in turn, obesity increased SDB risk. Both short sleep duration and SDB risk were significantly associated with behavioral problems in school.

DOI： 10.1093/sleep/zsw082

Scopus

再専門職化の時代における教員養成の方向性

丸山和昭

日本教育学会大會研究発表要項 76 巻 ( 0 ) 頁： 260 - 261 2017年

Optical testing of lightweight large all-C/SiC optics

Suganuma M., Imai T., Katayama H., Naitoh M., Tange Y., Yui Y.Y., Maruyama K., Kaneda H., Nakagawa T., Kotani M.

Proceedings of SPIE - The International Society for Optical Engineering 10565 巻 2017年

出版者・発行元：Proceedings of SPIE - The International Society for Optical Engineering

We carried out various tests of 800-mm-diameter aperture, lightweight optics that consisted wholly of carbon fiber-reinforced SiC composite, called HB-Cesic. A cryogenic optical test was performed on the primary mirror to examine any CTE irregularity as a surface change, and only small deformations were observed. The primary mirror was assembled with a convex secondary mirror into an optical system and tested under vacuum at the 6-m-diameter radiometer space chamber at Tsukuba Space Center of JAXA, where we have prepared interferometric metrological facilities to establish techniques to test large optical systems in a horizontal light-axis configuration. The wavefront difference between under vacuum and under atmosphere was confirmed to be less than 0.1 λ at λ=633 nm, if we realigned the optical axis of the interferometer and flat mirror under vacuum. We also demonstrated a stitching interferometry using the Φ800-mm optics by rotating a mask wheel of subapertures in front of the optical reference flat. The wavefront stitched from eight individual measurements of Φ275-mm subapertures differs from the full-aperture measurement without the mask by about 0.1 λ nm RMS, which showed the technique could able to be applied to test large telescopes especially for infrared wavelength region.

DOI： 10.1117/12.2309218

Scopus

Evaluation of tsunami force acting on bridge girders

Maruyama K., Tanaka Y., Kosa K., Hosoda A., Arikawa T., Mizutani N., Nakamura T.

American Concrete Institute, ACI Special Publication 2017-January 巻 ( SP 313 ) 頁： 23 - 32 2017年

出版者・発行元：American Concrete Institute, ACI Special Publication

This paper deals with what tsunami force acted on bridge girders by Great East Japan Earthquake broken out in March 11, 2011. First of all, a lot of efforts were conducted to collect almost all data of bridge girders in the inundation area. Satellite images in internet websites proved very effective to make a quick survey of any bridge in the inundation area. Detailed data on the bridges were obtained from authorities for use in damage analysis. The method proposed by Prof. Kosa was introduced to see whether bridges were washed away or not by tsunami. The resistance of each bridge is expressed as a function of the self-weight of bridge girder, and the tsunami force is defined by hydro-dynamic equation. More than two hundred bridges, including both washed away bridges and survived ones, were analyzed. Motion pictures taken by digital camera during the attack of tsunami were examined to evaluate the velocity and height of tsunami. As far as bridges in a local area were taken into account, the method was good to see whether bridges were survived or not. Field survey of the bridges washed away indicated that the failures would have been by tsunami uplift force. Experimental investigation was done using a water channel to examine what forces acted on bridge girders and to develop a proper simulation model.

Scopus

Frame-Based Behavior Preservation in Refactoring

Maruyama, K; Hayashi, S; Yoshida, N; Choi, E

2017 IEEE 24TH INTERNATIONAL CONFERENCE ON SOFTWARE ANALYSIS, EVOLUTION, AND REENGINEERING (SANER) 頁： 573 - 574 2017年

学問の自由と専門職の自律性 : アメリカ大学教授連合(AAUP)の運動史に基づく一考察

丸山和昭

教育社会学研究 114 巻 2024年7月

Digitalization of Higher Education in Japan: Challenges and Reflections for Education Reform 査読有り

Tatsuya Tooka, Naoyoshi Uchida, Keigo Takenaga, Kazuaki Maruyama, Maki Kato

Journal of Comparative & International Higher Education 16 巻 ( 2 ) 2024年5月

学生は中学・高校におけるキャリア教育をどのように評価しているのか : 中等教育におけるキャリア教育、進路指導、特別活動の経験に注目して

菊池美由紀, 東岡達也, 丸山和昭

愛知淑徳大学論集. 教育学研究科篇 14 巻 2024年3月

医学の専門分化はどのように起こり、進んできたのか : George Weisz., 2006, Divide and Conquerにおける議論を中心に招待有り査読有り

丸山和昭

名古屋大学大学院教育発達科学研究科紀要 2023年9月

医学の専門分化はどのように起こり、進んできたのか : George Weisz., 2006, Divide and Conquerにおける議論を中心に

丸山和昭

名古屋大学大学院教育発達科学研究科紀要. 教育科学 70 巻 ( 1 ) 2023年9月

学習の選択肢の拡大に伴うリスクと対策 : シンポジウムでのディスカッションを踏まえて招待有り査読有り

2022年3月

学習の選択肢の拡大に伴うリスクと対策―シンポジウムでのディスカッションを踏まえて―

安部有紀子, 丸山和昭

名古屋高等教育研究 22 巻 2022年3月

英米豪における大学教職員像の変容と日本への示唆招待有り査読有り

大学教育学会誌 2021年12月

英米豪における大学教職員像の変容と日本への示唆査読有り

杉本和弘, 大森不二雄, 森利枝, 丸山和昭, 夏目達也

大学教育学会誌 43 巻 ( 2 ) 2021年12月

各国比較にもとづく日本の教員業務と勤務時間管理の特質 (特集世界の教員の働き方 : 勤務時間管理と業務比較から) 招待有り

丸山和昭

季刊教育法 208 巻頁： 42 - 49 2021年3月

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