Faculty Profiles - YOSHIOKA, Hirofumi

Papers - YOSHIOKA, Hirofumi

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*StCDPK5 confers resistance to late blight pathogen but increases susceptibility to early blight pathogen in potato via reactive oxygen species burst. Reviewed

Kobayashi, M, Yoshioka, M, Asai, S, Nomura, H, Kuchimura, K, Mori, H, Doke, N, Yoshioka, H

New Phytologist Vol. 196 page： 223–237. 2012.10

StCDPK5 confers resistance to late blight pathogen but increases susceptibility to early blight pathogen in potato via reactive oxygen species burst

Kobayashi, M; Yoshioka, M; Asai, S; Nomura, H; Kuchimura, K; Mori, H; Doke, N; Yoshioka, H

NEW PHYTOLOGIST Vol. 196 ( 1 ) page： 223 - 237 2012.10

Ethylene Signaling Pathway and MAPK Cascades Are Required for AAL Toxin-Induced Programmed Cell Death

Mase, K; Mizuno, T; Ishihama, N; Fujii, T; Mori, H; Kodama, M; Yoshioka, H

MOLECULAR PLANT-MICROBE INTERACTIONS Vol. 25 ( 8 ) page： 1015 - 1025 2012.8

Post-translational regulation of WRKY transcription factors in plant immunity Invited Reviewed

Nobuaki Ishihama, Hirofumi Yoshioka

CURRENT OPINION IN PLANT BIOLOGY Vol. 15 ( 4 ) page： 431 - 437 2012.8

Language：English Publishing type：Research paper (scientific journal) Publisher：CURRENT BIOLOGY LTD

Plants have evolved immune system to protect themselves against invading pathogens. Recent research has illustrated that signaling networks, after perception of diverse pathogen-derived signals, facilitate transcriptional reprogramming through mitogen-activated protein kinase (MAPK) cascades. WRKY proteins, which comprise a large family of plant transcription factors, are key players in plant immune responses. WRKY transcription factors participate in the control of defense-related genes either as positive or as negative regulators, and essentially are regulated at the transcriptional level. Emerging evidence emphasizes that group I WRKY transcription factors, which contain a conserved motif in the N-terminal region, are also activated by MAPK-dependent phosphorylation, underlining their importance in plant immunity.

DOI： 10.1016/j.pbi.2012.02.003

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PubMed

Cell Death of Nicotiana benthamiana Is Induced by Secreted Protein NIS1 of Colletotrichum orbiculare and Is Suppressed by a Homologue of CgDN3

Yoshino, K; Irieda, H; Sugimoto, F; Yoshioka, H; Okuno, T; Takano, Y

MOLECULAR PLANT-MICROBE INTERACTIONS Vol. 25 ( 5 ) page： 625 - 636 2012.5

Observation of Riemannian geometric effects on electronic states

Onoe, J; Ito, T; Shima, H; Yoshioka, H; Kimura, S

EPL Vol. 98 ( 2 ) 2012.4

Isolation and characterization of an asparagine-rich protein that regulates hypersensitive cell death-mediated resistance in Nicotiana plants Reviewed

Daisuke Komori, Masahiro Nishihara, Akiko Takahashi, Meenu Gupta, Hirofumi Yoshioka, Hiroyuki Mizumoto, Kouhei Ohnishi, Yusufumi Hikichi, Akinori Kiba

PLANT BIOTECHNOLOGY Vol. 29 ( 3 ) page： 293 - 300 2012

Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOC PLANT CELL & MOLECULAR BIOLOGY

We isolated and characterized Ralstonia solanacearum-responsive genes (RsRGs) related to the hypersensitive response (HR) by virus-induced gene silencing using Nicotiana benthamiana and the potato virus x vector system. We focused on RsRGM10, because induction of HR was delayed in RsRGM10-silenced plants challenged with incompatible R. solanacearum 8107, which induces the HR in, N. benthamiana. The amino acid sequence deduced from the full-length RsRGM10 cDNA showed approximately 90% identity with rolB overexpressed 1 (ROX1) from N. tabacum, and contained an asparagine-rich sequence. We designated this protein NbARP (N. benthamiana asparagine-rich protein). Expression of HR-related hin1 and onset of the oxidative burst were delayed in NbARP-silenced plants, and induction of myelin basic kinase activity was reduced. Growth of R. solanacearum 8107 was accelerated in NbARP-silenced plants. Induction of the HR by Pseudomonas cichorii and Pseudomonas syringae pv. syringae was also delayed in NbARP-silenced plants. Silencing of NbARP reduced induction of HR-cell death by Agrobacterium tumefaciens-mediated transient expression of HR elicitors and a constitutively active form of mitogen activated protein kinase kinase. The population of R. solanacearum 8107 was decreased in NbARP-overexpressing plants. These results suggest that NbARP is closely related to the HR.

DOI： 10.5511/plantbiotechnology.12.0213b

Web of Science

SHAPE OPTIMIZATION OF THERMOELASTIC FIELDS FOR STIFFNESS MAXIMIZATION

Katamine, E; Yoshioka, H; Azegami, H

PROCEEDINGS OF THE ASME INTERNATIONAL DESIGN ENGINEERING TECHNICAL CONFERENCES AND COMPUTERS AND INFORMATION IN ENGINEERING CONFERENCE 2012, VOL 2, PTS A AND B page： 853 - + 2012

NINJAはコリプレッサーTOPLESSとジャスモン酸シグナルをつなぐ―植物ホルモンの受容による新しい転写制御機構の役割は？ Invited

野村裕也, 吉岡博文

化学と生物 Vol. 49 page： 85-87 2011.2

Nitric oxide and reactive oxygen species in plant defense responses Invited

Yoshioka H, Asai S, Yoshioka M, Kobayashi M, Doke N

In Genome-Enabled Integration of Research in Plant Pathogen Systems. (Wolpert, T., Shiraishi, T., Allen, C., Glazebrook, J. and Akimitsu, K. eds), APS Press page： 47 - 55 2011

Analyses of the cis-Regulatory Regions Responsible for the Transcriptional Activation of the N Resistance Gene by Tobacco mosaic virus Reviewed

Michie Kobayashi, Nobuaki Ishihama, Hirofumi Yoshioka, Reona Takabatake, Shinya Tsuda, Shigemi Seo, Yuko Ohashi, Ichiro Mitsuhara

JOURNAL OF PHYTOPATHOLOGY Vol. 158 ( 11-12 ) page： 826 - 828 2010.12

Language：English Publishing type：Research paper (scientific journal) Publisher：WILEY-BLACKWELL

Although the tobacco N gene, which confers resistance to Tobacco mosaic virus (TMV), is transcriptionally induced by infection with TMV, the regulatory mechanism has not previously been elucidated. We found that a 5'-flanking region of the N gene exhibits TMV-inducible promoter activity and that the region from -290 to -271 includes a cis-acting element in response to infection. The activity required N protein and signal components of N-mediated resistance. These results suggest that transcription of the N gene is positively regulated by N-mediated resistance signalling.

DOI： 10.1111/j.1439-0434.2010.01686.x

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Regulation of Arabidopsis defense responses against Spodoptera littoralis by CPK-mediated calcium signaling Reviewed

Chidananda Nagamangala Kanchiswamy, Hirotaka Takahashi, Stefano Quadro, Massimo E. Maffei, Simone Bossi, Cinzia Bertea, Simon Atsbaha Zebelo, Atsushi Muroi, Nobuaki Ishihama, Hirofumi Yoshioka, Wilhelm Boland, Junji Takabayashi, Yaeta Endo, Tatsuya Sawasaki, Gen-ichiro Arimura

BMC PLANT BIOLOGY Vol. 10 page： 97 2010.5

Language：English Publishing type：Research paper (scientific journal) Publisher：BIOMED CENTRAL LTD

Background: Plant Ca(2+) signals are involved in a wide array of intracellular signalling pathways after pest invasion. Ca(2+)-binding sensory proteins such as Ca(2+)-dependent protein kinases (CPKs) have been predicted to mediate the signaling following Ca(2+) influx after insect herbivory. However, until now this prediction was not testable.
Results: To investigate the roles CPKs play in a herbivore response-signaling pathway, we screened the characteristics of Arabidopsis CPK mutants damaged by a feeding generalist herbivore, Spodoptera littoralis. Following insect attack, the cpk3 and cpk13 mutants showed lower transcript levels of plant defensin gene PDF1.2 compared to wild-type plants. The CPK cascade was not directly linked to the herbivory-induced signaling pathways that were mediated by defense-related phytohormones such as jasmonic acid and ethylene. CPK3 was also suggested to be involved in a negative feedback regulation of the cytosolic Ca(2+) levels after herbivory and wounding damage. In vitro kinase assays of CPK3 protein with a suite of substrates demonstrated that the protein phosphorylates transcription factors (including ERF1, HsfB2a and CZF1/ZFAR1) in the presence of Ca(2+). CPK13 strongly phosphorylated only HsfB2a, irrespective of the presence of Ca(2+). Furthermore, in vivo agroinfiltration assays showed that CPK3-or CPK13-derived phosphorylation of a heat shock factor (HsfB2a) promotes PDF1.2 transcriptional activation in the defense response.
Conclusions: These results reveal the involvement of two Arabidopsis CPKs (CPK3 and CPK13) in the herbivory-induced signaling network via HsfB2a-mediated regulation of the defense-related transcriptional machinery. This cascade is not involved in the phytohormone-related signaling pathways, but rather directly impacts transcription factors for defense responses.

DOI： 10.1186/1471-2229-10-97

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PubMed

S-Glycoprotein-Like Protein Regulates Defense Responses in Nicotiana Plants against Ralstonia solanacearum Reviewed

Milimo Maimbo, Kouhei Ohnishi, Yasufumi Hikichi, Hirofumi Yoshioka, Akinori Kiba

PLANT PHYSIOLOGY Vol. 152 ( 4 ) page： 2023 - 2035 2010.4

Language：English Publishing type：Research paper (scientific journal) Publisher：AMER SOC PLANT BIOLOGISTS

RsRGA4 (for Ralstonia solanacearum-responsive gene A4) encodes a polypeptide similar to S-locus glycoprotein (SGP) from Brassica rapa and SGP-like proteins from Ipomoea trifida and Medicago truncatula. Therefore, we designated RsRGA4 as NtSGLP (for Nicotiana tabacum SGP-like protein) and NbSGLP (its Nicotiana benthamiana ortholog). NbSGLP is expressed in root, leaf, petal, gynoecium, and stamen. Expression of NbSGLP was strongly induced by inoculation with an avirulent strain of R. solanacearum (Rs8107) and slightly enhanced by inoculation with virulent R. solanacearum (RsOE1-1). Expression of NbSGLP was induced by inoculation with an hrpY-deficient mutant of RsOE1-1 and Rs8107. Expression was also induced by aminocyclopropane carboxylic acid and salicylic acid. Virus-induced gene silencing of NbSGLP enhanced the growth of Rs8107. Growth of RsOE1-1 and appearance of wilt symptoms were also accelerated in silenced plants. Expression of PR-1a and EREBP was reduced, and markers for basal defense, such as callose deposition and reduced vascular flow, were compromised in NbSGLP-silenced plants. Moreover, growth of Pseudomonas cichorii, Pseudomonas syringae pv tabaci, and P. syringae pv mellea was also enhanced in the silenced plants. On the other hand, silencing of NbSGLP did not interfere with the appearance of the hypersensitive response. NbSGLP was secreted in a signal peptide-dependent manner. Agrobacterium tumefaciens-mediated expression of NbSGLP induced PR-1a and EREBP expression, callose deposition, and reduced vascular flow. NbSGLP-induced callose deposition and reduced vascular flow were not observed in salicylic acid-deficient N. benthamiana NahG plants. Taken together, SGLP might have a role in the induction of basal defense in Nicotiana plants.

DOI： 10.1104/pp.109.148189

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PubMed

Ca2+-dependent protein kinases and their substrate HsfB2a are differently involved in the heat response signaling pathway in Arabidopsis Reviewed

Chidananda Nagamangala Kanchiswamy, Atsushi Muroi, Massimo E. Maffei, Hirofumi Yoshioka, Tatsuya Sawasaki, Gen-ichiro Arimura

PLANT BIOTECHNOLOGY Vol. 27 ( 5 ) page： 469 - 473 2010

Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOC PLANT CELL & MOLECULAR BIOL

Little is known about the mechanisms by which Ca2+-binding sensory proteins direct the plant heat shock (HS) response. Since two Ca2+-dependent protein kinases (CPK3 and CPK13) were recently shown to phosphorylate the heat shock transcription factor HsfB2a, we assessed in the current study whether these kinases are also involved in HS signal transduction, by monitoring the transcriptional profile of HS protein (Hsp) family genes in Arabidopsis Col-0 plants (WT) and the corresponding mutants. Both with and without HS, the gene transcript levels of Hsp70, Hsp101, Hsp17.4-CIII and Hsp15.7-CI were found to be lower in cpk3 and cpk13 mutants compared to WT, resulting in the impairment of basal thermotolerance in the mutants. To determine the in vivo function of CPKs, CPK3/13 and their substrate HsfB2a (heat shock transcription factor) were co-expressed as cofactors for the transient expression of a reporter (GUS) gene under the control of heat shock element (HSE) in Nicotiana benthamiana leaves. However, CPK3/13-phosphorylated HsfB2a did not function in the suppression/activation of HSE-promoted expression in the transient expression system. Implications for possible signal trafficking via CPKs and Hsfs are discussed.

DOI： 10.5511/plantbiotechnology.10.0728b

Web of Science

Remotely operable compact instruments for measuring atmospheric CO2 and CH4 column densities at surface monitoring sites

Kobayashi, N; Inoue, G; Kawasaki, M; Yoshioka, H; Minomura, M; Murata, I; Nagahama, T; Matsumi, Y; Tanaka, T; Morino, I; Ibuki, T

ATMOSPHERIC MEASUREMENT TECHNIQUES Vol. 3 ( 4 ) page： 1103 - 1112 2010

病害抵抗性・感受性決定に関わる植物の感染応答機構 Invited

木場章範, 中野真人, 伊藤慎, Maimbo, M, 大西浩平, 吉岡博文, 水本祐之, 曵地康史

農業現場の問題解決に向けた感染生理学 Vol. 46 page： 1-8 2010

Role of nitric oxide and reactive oxygen species in disease resistance to necrotrophic pathogens. Invited Reviewed

Asai, S, Mase, K, Yoshioka, H

Plant Signaling and Behavior Vol. 5 page： 872-874 2010

Riboflavin synthesis participates in plant immune responses in Nicotiana benthamiana Invited

Asai S, Mase K, Yoshioka H

In Biology of Plant-Microbe Interactions Vol. 7. Chapter 28, Proceedings of the 14th International Congress on Molecular Plant-Microbe Interactions. (Antoun, H., Avis, T., Brisson, L., Prevost, D. and Trepanier, M. eds), IS-MPMI Press, MN 2010

Molecular mechanisms of generation for nitric oxide and reactive oxygen species, and role of the radical burst in plant immunity Invited Reviewed

Hirofumi Yoshioka, Shuta Asai, Miki Yoshioka, Michie Kobayashi

MOLECULES AND CELLS Vol. 28 ( 4 ) page： 321 - 329 2009.10

Language：English Publishing type：Research paper (scientific journal) Publisher：KOREAN SOC MOLECULAR & CELLULAR BIOLOGY

Rapid production of nitric oxide (NO) and reactive oxygen species (ROS) has been implicated in the regulation of innate immunity in plants. A potato calcium-dependent protein kinase (StCDPK5) activates an NADPH oxidase StRBOHA to D by direct phosphorylation of N-terminal regions, and heterologous expression of StCDPK5 and StRBOHs in Nicotiana benthamiana results in oxidative burst. The transgenic potato plants that carry a constitutively active StCDPK5 driven by a pathogen-inducible promoter of the potato showed high resistance to late blight pathogen Phytophthora infestans accompanied by HR-like cell death and H(2)O(2) accumulation in the attacked cells. In contrast, these plants showed high susceptibility to early blight necrotrophic pathogen Alternaria solani, suggesting that oxidative burst confers high resistance to biotrophic pathogen, but high susceptibility to necrotrophic pathogen. NO and ROS synergistically function in defense responses. Two MAPK cascades, MEK2-SIPK and cytokinesis-related MEK1-NTF6, are involved in the induction of NbRBOHB gene in N. benthamiana. On the other hand, NO burst is regulated by the MEK2-SIPK cascade. Conditional activation of SIPK in potato plants induces oxidative and NO bursts, and confers resistance to both biotrophic and necrotrophic pathogens, indicating the plants may have obtained during evolution the signaling pathway which regulates both NO and ROS production to adapt to wide-spectrum pathogens.

DOI： 10.1007/s10059-009-0156-2

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PubMed

INF1 Elicitin Activates Jasmonic Acid- and Ethylene-mediated Signalling Pathways and Induces Resistance to Bacterial Wilt Disease in Tomato Reviewed

Yoko Kawamura, Shu Hase, Shigehito Takenaka, Yoshinori Kanayama, Hirofumi Yoshioka, Sophien Kamoun, Hideki Takahashi

JOURNAL OF PHYTOPATHOLOGY Vol. 157 ( 5 ) page： 287 - 297 2009.5

Language：English Publishing type：Research paper (scientific journal) Publisher：WILEY-BLACKWELL PUBLISHING, INC

Phytophthora infestans, the cause of potato and tomato late blight disease, produces INF1 elicitin, a 10 kDa extracellular protein. INF1 induces a hypersensitive response (HR) and systemic acquired resistance in species of the Nicotiana genus and a few other genera. We analysed the response of tomato to INF1 and INF1S3, which has a Cys to Ser substitution at position 3 of the processed protein and therefore lacks HR induction activity in tobacco. No HR cell death was induced in either INF1- or INF1S3-treated tomato leaves. The expression of salicylic acid (SA)-responsive PR-1a(P6) and PR-2a genes was not induced by treatment with either INF1 or INF1S3. However, the expression of jasmonic acid (JA)-responsive PR-6 encoding proteinase inhibitor II, LeATL6 encoding ubiquitin ligase E3, and LOX-E encoding lipoxygenase, was up-regulated in tomato leaves treated with INF1 but not in those treated with INF1S3. Their induction was completely compromised in INF1-treated jai1-1 mutant tomato, in which the JA signalling pathway is impaired. The accumulation of ethylene (ET) and the expression of ET-responsive genes were also induced in tomato by INF1 but not INF1S3 treatment. The activation of JA and ET-mediated signals but not the SA-mediated signalling in INF1-treated tomato was also demonstrated by global gene expression analysis. INF1-treated tomatoes, but not those treated with INF1S3, exhibited resistance to bacterial wilt disease caused by Ralstonia solanacearum. Thus, INF1 seems to induce resistance to bacterial wilt disease in tomato and activate JA- and ET-mediated signalling pathways without development of HR cell death.

DOI： 10.1111/j.1439-0434.2008.01489.x

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Papers - YOSHIOKA, Hirofumi