Faculty Profiles - YOSHIOKA, Hirofumi

Papers - YOSHIOKA, Hirofumi

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Disruption of AtPDR8, a plasma membrane ABC transporter of Arabidopsis, exhibits hypersensitive cell death by pathogen infection. Reviewed

Kobae, Y, Yoshioka, H, Sekino, T, Nakanishi, Y, Martinoia, E, Maeshima, M

Plant & Cell Physiology Vol. 47 page： 309-318 2006

Subcellular localization of Strboh proteins and NADPH-dependent O 2--generating activity in potato tuber tissues Reviewed

Michie Kobayashi, Kazuhito Kawakita, Masayoshi Maeshima, Noriyuki Doke, Hirofumi Yoshioka

Journal of Experimental Botany Vol. 57 ( 6 ) page： 1373 - 1379 2006

Language：English Publishing type：Research paper (scientific journal)

Rapid generation of reactive oxygen species (ROS) at the cell surface has been implicated in plant defence responses. Genetic evidence indicates that a plant NADPH oxidase (Rboh
respiratory burst oxidase homologue) is associated with oxidative burst. However, there is not enough physiological evidence of Rboh localization available yet. Isozyme-specific antibodies against potato StrbohA and StrbohB (St
Solanum tuberosum) were prepared to investigate the localization of these proteins. Immunoblot analyses using potato microsomal proteins revealed that StrbohA was expressed constitutively at a low level, whereas the accumulation of StrbohB protein was induced by the cell wall elicitor of the potato pathogen Phytophthora infestans. It is demonstrated here that StrbohA and StrbohB are distributed in plasma membrane fractions which have been separated by sucrose density-gradient centrifugation using their specific antibodies. Green fluorescent protein-tagged Strboh proteins were also located on the plasma membrane by transient expression assay in onion epidermal cells. Additionally, NADPH-dependent O2--generating activities in plasma membrane fractions were diphenylene iodonium-sensitive and NaN 3-insensitive. These data suggest that StrbohA and StrbohB are predominantly localized on the plasma membrane and regulate ROS production in defence signalling. © The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved.

DOI： 10.1093/jxb/erj113

Web of Science

Scopus

PubMed

植物の感染応答因子としてのNO． Invited

川北一人, 山本(加藤, 文子, 齋藤修平, 加藤新平, 加藤大明, 浅井秀太, 樹神博士, 吉岡博文, 道家紀志

病原体の寄生戦略と植物の応答 Vol. 42 page： 11-22 2006

Involvement of PPS3 phosphorylated by elicitor-responsive mitogen-activated protein kinases in the regulation of plant cell death Reviewed

S Katou, H Yoshioka, K Kawakita, O Rowland, JDG Jones, H Mori, N Doke

PLANT PHYSIOLOGY Vol. 139 ( 4 ) page： 1914 - 1926 2005.12

Language：English Publishing type：Research paper (scientific journal) Publisher：AMER SOC PLANT BIOLOGISTS

Mitogen-activated protein kinase (MAPK) cascades play pivotal roles in plant innate immunity. Overexpression of StMEK1(DD), a constitutively active MAPK kinase that activates salicylic acid-induced protein kinase (SIPK) and wound-induced protein kinase (WIPK), provokes hypersensitive response-like cell death in Nicotiana benthamiana. Here we purified a 51-kD MAPK, which was activated in potato (Solanum tuberosum) tubers treated with hyphal wall elicitor of a plant pathogen, and isolated the cDNA designated StMPK1. The deduced amino acid sequence of the StMPK1 showed strong similarity to stress-responsive MAPKs, such as tobacco (Nicotiana tabacum) SIPK and Arabidopsis (Arabidopsis thaliana) AtMPK6. To investigate the downstream signaling of StMPK1, we identified several proteins phosphorylated by StMPK1 (PPSs) using an in vitro expression cloning method. To dissect the biological function of PPSs in the plant defense, we employed virus-induced gene silencing (VIGS) in N. benthamiana. VIGS of NbPPS3 significantly delayed cell death induced by the transient expression of StMEK1(DD) and treatment with hyphal wall elicitor. Furthermore, the mobility shift of NbPPS3 on SDS-polyacrylamide gel was induced by transient expression of StMEK1(DD). The mobility shift of NbPPS3 induced by StMEK1(DD) was not compromised by VIGS of WIPK or SIPK alone, but drastically reduced by the silencing of both WIPK and SIPK. This work strongly supports the idea that PPS3 is a physiological substrate of StMPK1 and is involved in cell death activated by a MAPK cascade.

DOI： 10.1104/pp.105.066795

Web of Science

PubMed

抵抗性遺伝子を介した防御応答におけるシグナル伝達機構． Invited

吉岡博文, 山溝千尋, 小林光智衣, 水野貴仁, 川北一人, 道家紀志

植物・病原体相互反応における特異性決定機構 Vol. 41 page： 13-22 2005.8

The Arabidopsis gene CAD1 controls programmed cell death in the plant immune system and encodes a protein containing a MACPF domain Reviewed

C Morita-Yamamuro, T Tsutsui, M Sato, H Yoshioka, M Tamaoki, D Ogawa, H Matsuura, T Yoshihara, A Ikeda, Uyeda, I, J Yamaguchi

PLANT AND CELL PHYSIOLOGY Vol. 46 ( 6 ) page： 902 - 912 2005.6

Language：English Publishing type：Research paper (scientific journal) Publisher：OXFORD UNIV PRESS

To clarify the processes involved in plant immunity, we have isolated and characterized a single recessive Arabidopsis mutant, cad1 (constitutively activated cell death 1), which shows a phenotype that mimics the lesions seen in the hypersensitive response (HR). This mutant shows spontaneously activated expression of pathogenesis-related (PR) genes, and leading to a 32-fold increase in salicylic acid (SA). Inoculation of cad1 mutant plants with Pseudomonas syringae pv tomato DC3000 shows that the cad1 mutation results in the restriction of bacterial growth. Cloning of CAD1 reveals that this gene encodes a protein containing a domain with significant homology to the MACPF (membrane attack complex and perforin) domain of complement components and perforin proteins that are involved in innate immunity in animals. Furthermore, cell death is suppressed in transgenic cad1 plants expressing nahG, which encodes an SA-degrading enzyme. We therefore conclude that the CAD1 protein negatively controls the SA-mediated pathway of programmed cell death in plant immunity.

DOI： 10.1093/pcp/pci095

Web of Science

PubMed

Primary lung adenocarcinoma with heterotopic bone formation.

Usami N, Yoshioka H, Mori S, Imaizumi M, Nagasaka T, Ueda Y

The Japanese journal of thoracic and cardiovascular surgery : official publication of the Japanese Association for Thoracic Surgery = Nihon Kyobu Geka Gakkai zasshi Vol. 53 ( 2 ) page： 102 - 5 2005.2

Functional analysis of Avr9/Cf-9 rapidly elicited genes identifies a protein kinase, ACIK1, that is essential for full Cf-9-dependent disease resistance in tomato Reviewed

O Rowland, AA Ludwig, CJ Merrick, F Baillieul, FE Tracy, WE Durrant, L Fritz-Laylin, Nekrasov, V, K Sjolander, H Yoshioka, JDG Jones

PLANT CELL Vol. 17 ( 1 ) page： 295 - 310 2005.1

Language：English Publishing type：Research paper (scientific journal) Publisher：AMER SOC PLANT BIOLOGISTS

Tomato (Lycopersicon esculentum) Cf genes confer resistance to the fungal pathogen Cladospodium fulvum through recognition of secreted avirulence (Avr) peptides. Plant defense responses, including rapid alterations in gene expression, are immediately activated upon perception of the pathogen. Previously, we identified a collection of Avr9/Cf-9 rapidly (15 to 30 min) elicited (ACRE) genes from tobacco (Nicotiana tabacum). Many of the ACRE genes encode putative signaling components and thus may play pivotal roles in the initial development of the defense response. To assess the requirement of 42 of these genes in the hypersensitive response (HR) induced by Cf-9/Avr9 or by Cf-4/Avr4, we used virus-induced gene silencing (VIGS) in N. benthamiana. Three genes were identified that when silenced compromised the Cf-mediated HR. We further characterized one of these genes, which encodes a Ser/Thr protein kinase called Avr9/Cf-9 induced kinase 1 (ACIK1). ACIK1 mRNA was rapidly upregulated in tobacco and tomato upon elicitation by Avr9 and by wounding. Silencing of ACIK1 in tobacco resulted in a reduced HR that correlated with loss of ACIK1 transcript. Importantly, ACIK1 was found to be required for Cf-9/Avr9- and Cf-4/Avr4-mediated HRs but not for the HR or resistance mediated by other resistance/Avr systems, such as Pto/AvrPto, Rx/Potato virus X, or N/Tobacco mosaic virus. Moreover, VIGS of LeACIK1 in tomato decreased Cf-9-mediated resistance to C. fulvum, showing the importance of ACIK1 in disease resistance.

DOI： 10.1105/tpc.104.026013

Web of Science

Role of calcium ion in plant innate immunity

Yoshioka, H; Ooura, I; Kawakita, K; Doke, N

PLANT AND CELL PHYSIOLOGY Vol. 46 page： S23 - S23 2005

植物における活性酸素の役割とその誘導．活性酸素，－生産と防御のメカニズム－． Invited

吉岡博文, 山溝千尋

蛋白質核酸酵素 Vol. 50 page： 310-315 2005

Involvement of nitric oxide generation in hypersensitive cell death induced by elicitin in tobacco cell suspension culture.

Yamamoto, A, Katou, S, Yoshioka, H, Doke, N, Kawakita, K

Journal of Genaral Plant Pathology Vol. 70 page： 85-92 2004.1

Cytosolic HSP90 and HSP70 are essential components of INF1-mediated hypersensitive response and non-host resistance to Pseudomonas cichorii in Nicotiana benthamiana

Kanzaki, H; Saitoh, H; Ito, A; Fujisawa, S; Kamoun, S; Katou, S; Yoshioka, H; Terauchi, R

MOLECULAR PLANT PATHOLOGY Vol. 4 ( 5 ) page： 383 - 391 2003.9

Disease stress-inducible genes of tobacco: expression profile of elicitor-responsive genes isolated by subtractive hybridization

Takemoto, D; Yoshioka, H; Doke, N; Kawakita, K

PHYSIOLOGIA PLANTARUM Vol. 118 ( 4 ) page： 545 - 553 2003.8

Commensurate-incommensurate transition in a one-dimensional dimerized electron system close to quarter-filling

Yoshioka, H; Tsuchiizu, M; Suzumura, Y

SYNTHETIC METALS Vol. 133 page： 49 - 50 2003.3

Elicitation of primary and secondary metabolism during defense in the potato. Reviewed

Nakane, E, Kawakita, K, Doke, N, Yoshioka, H

Journal of Genaral Plant Pathology Vol. 69 page： 378-384 2003.1

Nitrate reductase, a nitric oxide-producing enzyme: induction by pathogen signals. Reviewed

Yamamoto, A, Katou, S, Yoshioka, H, Doke, N, Kawakita, K

Journal of Genaral Plant Pathology Vol. 69 page： 218-229 2003.1

Salmonella pericarditis in a patient with primary idiopathic chylopericardium.

Yoshioka H, Shigemitsu K, Takeuchi M, Mori S, Imaizumi M, Ueda Y

The Japanese journal of thoracic and cardiovascular surgery : official publication of the Japanese Association for Thoracic Surgery = Nihon Kyobu Geka Gakkai zasshi Vol. 51 ( 1 ) page： 16 - 7 2003.1

Direct interaction of OsRac with NADPH oxidase regulates ROS production in defense signaling of rice

Pinontoan, R; Yoshioka, H; Kawasaki, T; Shimamoto, K

PLANT AND CELL PHYSIOLOGY Vol. 44 page： S178 - S178 2003

Functional analysis of potato mitogen-activated protein kinase kinase, StMEK1. Reviewed

Katou, S, Yamamoto, A, Yoshioka. H, Kawakita, K, Doke, N

Journal of General Plant Pathology Vol. 69 ( 3 ) page： 161-168 - 168 2003

宿主植物の抵抗性制御と糸状菌病に対する耐病性強化の展望． Invited

吉岡博文, 口村和男, 山溝千尋, 池田直希, 加藤新平, 川北一人, 小林晃, 道家紀志

作物の耐病性強化戦略と植物－病原体相互作用の分子機構研究 Vol. 39 page： 101-110 2003

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Papers - YOSHIOKA, Hirofumi