Faculty Profiles - YOSHIOKA, Hirofumi

Papers - YOSHIOKA, Hirofumi

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NADPH oxidase-mediated sulfenylation of cysteine derivatives regulates plant immunity Reviewed

Hino, Y., Inada, T., Yoshioka, M. and Yoshioka, H.

Journal of Experimental Botany Vol. 75 ( 15 ) page： 4641 - 4657 2024

CDPK5 and CDPK13 play key roles in acclimation to low oxygen through the control of RBOH-mediated ROS production in rice. Reviewed

Li, J., Ishii, T., Yoshioka, M., Hino, Y., Nomoto, M., Tada, Y., Yoshioka, H., Takahashi, H., Yamauchi, T. and Nakazono, M.

Plant Physiology Vol. kiae293 2024

Dynamics of plant immune MAPK activity and ROS signaling in response to invaders. Invited Reviewed

Yoshioka, H., Hino, Y., Iwata, K., Ogawa, T., Yoshioka, M., Ishihama, N. and Adachi, H.

Physiological and Molecular Plant Pathology 2023.3

Oral RNAi of diap1 results in rapid reduction of damage to potatoes in Henosepilachna vigintioctopunctata. Reviewed

Chikami, Y., Kawaguchi, H., Suzuki, T., Yoshioka, H., Sato, Y., Yaginuma, T. and Niimi, T.

Journal of Pest Science Vol. 94 page： 505 - 515 2021.4

Role of salicylic acid glucosyltransferase in balancing growth and defence for optimum plant fitness. Reviewed

Kobayashi, Y., Fukuzawa, N., Hyodo, A., Kim, H., Mashiyama, S., Ogihara, T., Yoshioka, H., Matsuura, H., Masuta, C., Matsumura, T. and Takeshita, M.

Molecular Plant Pathology Vol. 21 page： 429-442. 2020.3

RNAi of sesquiterpene cyclase gene for phytoalexin production impairs in pre- and postinvasive resistance to potato blight pathogens. Reviewed

Yoshioka, M, Adachi, I, Sato, Y, Doke, N, Kondo, T, Yoshioka, H

Molecular Plant Pathology Vol. 20 ( 7 ) page： 907-922. - 922 2019.4

An NADPH oxidase RBOH functions in rice roots during lysigenous aerenchyma formation under oxygen-deficient conditions. Reviewed

Yamauchi, T., Yoshioka, M., Fukazawa, A., Mori, H., Nishizawa, N.K., Tsutsumi, N., Yoshioka, H. and Nakazono, M.

The Plant Cell Vol. 29 page： 775-790. 2017.4

Nicotiana benthamiana MAPK-WRKY pathway confers resistance to a necrotrophic pathogen Botrytis cinerea. Reviewed

Adachi, H., Ishihama, N., Nakano, T., Yoshioka, M. and Yoshioka, H.

Plant Signaling & Behavior Vol. 6 ( 11 ) page： e1183085 2016.5

Hierarchical regulation of NADPH oxidase by protein kinases in plant immunity. Invited Reviewed

Yoshioka, H., Adachi, H., Nakano, T., Miyagawa, N., Ishihama, N., Asai, S. and Yoshioka, M.

Physiological and Molecular Plant Pathology Vol. 95 page： 20-26. 2016.5

SGT1 contributes to maintaining protein levels of MEK2^DD to facilitate hypersensitive response-like cell death in Nicotiana benthamiana. Reviewed

Ichimura, K., Shinzato, T., Edaki, M., Yoshioka, H. and Shirasu, K.

Physiological and Molecular Plant Patholology Vol. 94 page： 47-52. 2016.4

*WRKY transcription factors phosphorylated by MAPK regulate a plant immune NADPH oxidase in Nicotiana benthamiana. Reviewed

Adachi, H., Nakano, T., Miyagawa, N., Ishihama, N., Yoshioka, M., Katou, Y., Yaeno, T., Shirasu, K. and Yoshioka, H.

The Plant Cell Vol. 27 ( 9 ) page： 2645-2663. 2015.10

Molecular Characterization of LjABCG1, an ATP-Binding Cassette Protein in Lotus japonicus Reviewed

Akifumi Sugiyama, Shoju Fukuda, Kojiro Takanashi, Miki Yoshioka, Hirofumi Yoshioka, Yoshihiro Narusaka, Mari Narusaka, Mikiko Kojima, Hitoshi Sakakibara, Nobukazu Shitan, Shusei Sato, Satoshi Tabata, Masayoshi Kawaguchi, Kazufumi Yazaki

PLOS ONE Vol. 10 ( 9 ) page： e0139127. 2015.9

Language：English Publishing type：Research paper (scientific journal) Publisher：PUBLIC LIBRARY SCIENCE

LjABCG1, a full-size ABCG subfamily of ATP-binding cassette proteins of a model legume, Lotus japonicus, was reported as a gene highly expressed during the early stages of nodulation, but have not been characterized in detail. In this study we showed that the induction of LjABCG1 expression was remarkable by methyl jasmonate treatment, and reporter gene experiments indicated that LjABCG1 was strongly expressed in the nodule parenchyma and cell layers adjacent to the root vascular tissue toward the nodule. LjABCG1 was suggested to be localized at the plasma membrane based on the fractionation of microsomal membranes as well as separation via aqueous two-phase partitioning. The physiological functions of LjABCG1 in symbiosis and pathogenesis were analyzed in homologous and heterologous systems. LjABCG1 knock-down L. japonicus plants did not show clear phenotypic differences in nodule formation, and not in defense against Pseudomonas syringae, either. In contrast, when LjABCG1 was expressed in the Arabidopsis pdr8-1 mutant, the penetration frequency of Phytophthora infestans, a potato late blight pathogen, was significantly reduced in LjABCG1/pdr8-1 than in pdr8-1 plants. This finding indicated that LjABCG1, at least partially, complemented the phenotype of pdr8 in Arabidopsis, suggesting the multiple roles of this protein in plant-microbe interactions.

DOI： 10.1371/journal.pone.0139127

Web of Science

PubMed

Cell death-inducing stresses are required for defense activation in DS1-phosphatidic acid phosphatase-silenced Nicotiana benthamiana. Reviewed

Nakano, M., Yoshioka, H., Ohnishi, K., Hikichi, Y. and Kiba, A.

Journal of Plant Physiology Vol. 184 page： 15-19. 2015.7

*A receptor pair with an integrated decoy converts pathogen disabling of transcription factors to immunity. Reviewed

Le Roux, C., Huet, G., Jauneau, A., Camborde, L., Trémousaygue, D., Kraut, A., Zhou, B., Levaillant, M., Adachi, H., Yoshioka, H., Raffaele, S., Berthomé, R., Couté, Y., Parker, J.E. and Deslandes, L.

Cell Vol. 161 page： 1074-1088. 2015.5

Kinase-mediated orchestration of NADPH oxidase in plant immunity. Invited Reviewed

Adachi, H. and Yoshioka, H.

Briefings in Functional Genomics Vol. 14 ( 4 ) page： 253-259. 2015.3

リン酸化反応が制御するROSバーストの分子機構 Invited Reviewed

吉岡博文・安達広明・石濱伸明・中野孝明・白石佑太郎・宮川典子・野村裕也・吉岡美樹・浅井秀太

日本植物病理学会報 Vol. 81 page： 1-8. 2015.2

Map-based Cloning and Characterization of a Brown Planthopper Resistance Gene BPH26 from Oryza sativa L. ssp indica Cultivar ADR52 Reviewed

Yasumori Tamura, Makoto Hattori, Hirofumi Yoshioka, Miki Yoshioka, Akira Takahashi, Jianzhong Wu, Naoki Sentoku, Hideshi Yasui

SCIENTIFIC REPORTS Vol. 4 page： 5872 2014.7

Language：English Publishing type：Research paper (scientific journal) Publisher：NATURE PUBLISHING GROUP

The brown planthopper (BPH) is the most serious insect pest of rice in Asia. The indica rice cultivar ADR52 carries two BPH resistance genes, BPH26 (BROWN PLANTHOPPER RESISTANCE 26) and BPH25. Map-based cloning of BPH26 revealed that BPH26 encodes a coiled-coil-nucleotide-binding-site-leucine-rich repeat (CC-NBS-LRR) protein. BPH26 mediated sucking inhibition in the phloem sieve element. BPH26 was identical to BPH2 on the basis of DNA sequence analysis and feeding ability of the BPH2-virulent biotype of BPH. BPH2 was widely incorporated in elite rice cultivars and was well-cultivated in many Asian countries as a favorable gene resource in rice breeding against BPH. However, BPH2 was rendered ineffective by a virulent biotype of BPH in rice fields in Asia. In this study, we suggest that BPH2 can be reused by combining with other BPH resistance genes, such as BPH25, to ensure durable resistance to BPH.

DOI： 10.1038/srep05872

Web of Science

PubMed

SEC14 phospholipid transfer protein is involved in lipid signaling-mediated plant immune responses in Nicotiana benthamiana. Reviewed

Kiba, A., Galis, I., Hojo, Y., Ohnishi, K., Yoshioka, H. and Hikichi, Y.

PLoS ONE Vol. 9 ( 5 ) page： e98150 2014.5

Silencing of DS2 aminoacylase-like genes confirms basal resistance to Phytophthora infestans in Nicotiana benthamiana. Reviewed

Nakano, M., Nishihara, M., Yoshioka, H., Ohnishi, K., Hikichi, Y. and Kiba, A.

Plant Signal. Behav. Vol. 9 ( 2 ) page： e28004 2014.2

Suppression of DS1 phosphatidic acid phosphatase confirms resistance to Ralstonia solanacearum in Nicotiana benthamiana. Reviewed

Nakano, M., Nishihara, M., Yoshioka, H., Takahashi, H., Sawasaki, T., Ohnishi, K., Hikichi, Y. and Kiba, A.

PLoS ONE Vol. 8 ( 9 ) page： e75124. 2013.9

A translationally controlled tumor protein negatively regulates the hypersensitive response in Nicotiana benthamiana. Reviewed

Gupta, M., Yoshioka, H., Ohnishi, K., Mizumoto, H., Hikichi, Y. and Kiba, A.

Plant and Cell Physiology Vol. 54 ( 8 ) page： 1403-1414. 2013.8

タンパク質のリン酸化によるROSバーストの分子機構 Invited

吉岡博文・安達広明・石濱伸明・中野孝明・白石佑太郎・宮川典子・野村裕也・吉岡美樹・浅井秀太

植物−微生物相互作用とビオトロフ感染 Vol. 48 page： 105-114. 2013.8

Ethylene responsive AP2/ERF transcription factor MACD1 participates in phytotoxin-triggered programmed cell death. Reviewed

Mase, K., Ishihama, N., Mori, H., Takahashi, H., Kaminaka, H., Kodama, M. and Yoshioka, H.

Molecular Plant-Microbe Interactions Vol. 26 page： 868-879. 2013.7

*The variable domain of a plant calcium-dependent protein kinase (CDPK) confers subcellular localization and substrate recognition for NADPH oxidase. Reviewed

Asai, S., Ichikawa, T., Nomura, H., Kobayashi, M., Kamiyoshihara, Y., Mori, H., Kadota, Y., Zipfel, C., Jones, J.D.G. and Yoshioka, H.

The Journal of Biological Chemistry Vol. 288 page： 14332-14340. 2013.5

*A receptor-like cytoplasmic kinase targeted by a plant pathogen effector is directly phosphorylated by the chitin receptor and mediates rice immunity. Reviewed

Yamaguchi, K., Yamada, K., Ishikawa, K., Yoshimura, S., Hayashi, N., Uchihashi, K., Ishihama, N., Kishi-Kaboshi, M., Takahashi, A., Tsuge, S., Ochiai, H., Tada, Y., Shimamoto, K., Yoshioka, H. and Kawasaki, T.

Cell Host & Microbe Vol. 13 page： 347-357. 2013.3

Nitric oxide as a mediator for defense responses. Invited Reviewed

Bellin, D., Asai, S., Delledonne, M. and Yoshioka, H.

Molecular Plant-Microbe Interactions Vol. 26 ( 3 ) page： 271-277. 2013.2

*StCDPK5 confers resistance to late blight pathogen but increases susceptibility to early blight pathogen in potato via reactive oxygen species burst. Reviewed

Kobayashi, M., Yoshioka, M., Asai, S., Nomura, H., Kuchimura, K., Mori, H., Doke, N. and Yoshioka, H.

New Phytologist Vol. 196 page： 223–237. 2012.10

*Post-translational regulation of WRKY transcription factors in plant immunity. Invited Reviewed

Ishihama, N. and Yoshioka, H.

Current Opinion in Plant Biology Vol. 15 page： 431-437. 2012.8

Isolation and characterization of an asparagine-rich protein that regulates hypersensitive cell death-mediated resistance in Nicotiana plants. Reviewed

Komori, D., Nishihara, M., Takahashi, A., Gupta, M., Yoshioka, H., Mizumoto, H., Ohnishi, K., Hikichi, Y. and Kiba, A.

Plant Biotechnology Vol. 29 page： 293-300. 2012.7

Ethylene signaling pathway and MAPK cascades are required for AAL toxin-induced programmed cell death. Reviewed

Mase, K., Mizuno, T., Ishihama, N., Fujii, T., Mori, H., Kodama, M. and Yoshioka, H.

Molecular Plant-Microbe Interactions Vol. 25 page： 1015-1025. 2012.7

*Chloroplast-mediated activation of plant immune signalling in Arabidopsis. Reviewed

Nomura, H., Komori, T., Uemura, S., Kanda, Y., Shimotani, K., Nakai, K., Furuichi, T., Takebayashi, K., Sugimoto, T., Sano, S., Suwastika, I.N., Fukusaki, E., Yoshioka, H., Nakahira, Y. and Shiina, T.

Nature Communications Vol. 3 page： 926. 2012.6

A novel Sec14 phospholipid transfer protein from Nicotiana benthamiana is up-regulated in response to Ralstonia solanacearum infection, pathogen associated molecular patterns and effector molecules and involved in plant immunity. Reviewed

Kiba, A., Nakano, M., Vincent-Pope, P., Takahashi, H., Sawasaki, T., Endo, Y., Ohnishi, K., Yoshioka, H. and Hikichi, Y.

Journal of Plant Physiology Vol. 169 ( 10 ) page： 1017-1022. 2012.6

Cell death of Nicotiana benthamiana is induced by secreted protein NIS1 of Colletotrichum orbiculare and is suppressed by a homologue of CgDN3. Reviewed

Yoshino, K., Irieda, H., Sugimoto, F., Yoshioka, H., Okuno, T. and Takano, Y.

Molecular Plant-Microbe Interactions Vol. 25 page： 625-636. 2012.4

Regulatory mechanisms of nitric oxide and reactive oxygen species generation and their role in plant immunity. Invited Reviewed

Yoshioka, H., Mase, K., Yoshioka, M., Kobayashi, M. and Asai, S.

Nitric Oxide Vol. 25 page： 216-221. 2011.8

A plastidic glucose-6-phosphate dehydrogenase is responsible for hypersensitive response cell death and reactive oxygen species production Reviewed

Shuta Asai, Miki Yoshioka, Hironari Nomura, Chiyori Tone, Kazumi Nakajima, Eiichi Nakane, Noriyuki Doke, Hirofumi Yoshioka

JOURNAL OF GENERAL PLANT PATHOLOGY Vol. 77 ( 3 ) page： 152 - 162 2011.5

Language：English Publishing type：Research paper (scientific journal) Publisher：SPRINGER TOKYO

Glucose-6-phosphate dehydrogenase (G6PDH) has been implicated in the supply of reduced nicotine amide cofactors for resistance to biotic and abiotic stresses. Here, we show participation of the plastidic P2 isoform of G6PDH in plant immunity. A cytosolic isoform (NbG6PDH-Cyto) and two plastidic isoforms (NbG6PDH-P1 and NbG6PDH-P2) cloned from Nicotiana benthamiana were localized in cytosol and chloroplasts, respectively. Hypersensitive response (HR) cell death and NADPH oxidase (RBOH; respiratory burst oxidase homolog)-dependent reactive oxygen species (ROS) production after recognition of INF1 elicitin, secreted by oomycete Phytophthora infestans, decreased in NbG6PDH-P2-silenced plants, but not in NbG6PDH-Cyto- and NbG6PDH-P1-silenced plants. Silencing of the cytosolic NAD kinase NbNADK1, which phosphorylates NADH to form NADPH, compromised HR cell death and ROS production, and concomitant silencing with NbG6PDH-P2 reduced HR cell death and ROS to levels near those in NbG6PDH-P2-silenced plants. Similarly, silencing NbG6PDH-P2 and NbNADK1 resulted in high susceptibility to P. infestans. These results suggest that NADPH produced by the P2 isoform of G6PDH in chloroplasts is responsible for HR cell death and ROS production mediated by RBOH and that NbNADK1 is involved in this pathway.

DOI： 10.1007/s10327-011-0304-3

Web of Science

*Phosphorylation of the Nicotiana benthamiana WRKY8 transcription factor by MAPK functions in the defense response. Reviewed

Ishihama, N., Yamada, R., Yoshioka, M., Katou, S. and Yoshioka, H.

The Plant Cell Vol. 23 page： 1153-1170. 2011.3

NINJAはコリプレッサーTOPLESSとジャスモン酸シグナルをつなぐ―植物ホルモンの受容による新しい転写制御機構の役割は？ Invited

野村裕也・吉岡博文

化学と生物 Vol. 49 page： 85-87 2011.2

S-glycoprotein-like protein regulates defense responses in Nicotiana plants against Ralstonia solanacearum. Reviewed

Maimbo, M., Ohnishi, K., Hikichi, Y., Yoshioka, H. and Kiba, A.

Plant Physiology Vol. 152 page： 2023-2035 2010

Ca²⁺-dependent protein kinases and their substrate HsfB2a are differently involved in the heat response signaling pathway in Arabidopsis. Reviewed

Nagamangala Kanchiswamy, C., Maffei, M.E., Yoshioka, H., Sawasaki, T. and Arimura, G.

Plant Biotechnology Vol. 27 page： 469-473 2010

Regulation of Arabidopsis defense responses against Spodoptera littoralis by CPK-mediated calcium signaling. Reviewed

Nagamangala Kanchiswamy, C., Takahashi, H., Quadro, S., Maffei, M.E., Bossi, S., Bertea, C., Zebelo, S.A., Muroi, A., Ishihama, N., Yoshioka, H., Boland, W., Takabayashi, J., Endo, Y., Sawasaki, T. and Arimura, G.

BMC Plant Biology Vol. 10 page： 97 2010

Role of nitric oxide and reactive oxygen species in disease resistance to necrotrophic pathogens. Invited Reviewed

Asai, S., Mase, K. and Yoshioka, H.

Plant Signaling and Behavior Vol. 5 page： 872-874 2010

*A key enzyme for flavin synthesis is required for nitric oxide and reactive oxygen species production in disease resistance. Reviewed

Asai, S., Mase, K. and Yoshioka, H.

The Plant Journal Vol. 62 page： 911-924 2010

Analyses of the cis-regulatory regions responsible for the transcriptional activation of the N resistance gene by Tobacco mosaic virus. Reviewed

Kobayashi, M., Ishihama, N., Yoshioka, H., Takabatake, R., Tsuda, S., Seo, S., Ohashi, Y. and Mitsuhara, I.

Journal of Phytopathology Vol. 158 page： 826-828. 2010

病害抵抗性・感受性決定に関わる植物の感染応答機構 Invited

木場章範・中野真人・伊藤　慎・Maimbo, M.・大西浩平・吉岡博文・水本祐之・曵地康史

農業現場の問題解決に向けた感染生理学 Vol. 46 page： 1-8 2010

Nitric oxide as a partner of reactive oxygen species participates in disease resistance to necrotrophic pathogen Botrytis cinerea in Nicotiana benthamiana. Reviewed

Asai, S. and Yoshioka, H.

Molecular Plant-Microbe Interactions Vol. 22 page： 619-629 2009

Molecular mechanisms of generation for nitric oxide and reactive oxygen species, and role of the radical burst in plant immunity. Invited Reviewed

Yoshioka, H., Asai, S., Yoshioka, M. and Kobayashi, M.

Molecules and Cells Vol. 28 page： 321-329 2009

*The Colletotrichum orbiculare ssd1 mutant enhances Nicotiana benthamiana basal resistance by activating a mitogen-activated protein kinase pathway. Reviewed

Tanaka, S., Ishihama, N., Yoshioka, H., Huser, A., O'Connell, R., Tsuji, G., Tsuge, S. and Kubo, Y.

The Plant Cell Vol. 21 page： 2517-2526 2009

INF1 elicitin activates jasmonic acid- and ethylene-mediated signalling pathways and induces resistance to bacterial wilt disease in tomato. Reviewed

Kawamura, Y., Hase, S., Takenaka, S., Kanayama, Y., Yoshioka, H., Kamoun, S. and Takahashi, H.

Journal of Phytopathology Vol. 157 page： 287-297 2009

Vestigial and scalloped in the ladybird beetle: a conserved function in wing development and a novel function in pupal ecdysis. Reviewed

Ohde, T., Matsumoto, M., Morita-Miwa, M., Matsuura, H., Yoshioka, H., Yaginuma, T. and Niimi, T.

Insect Molecular Biology Vol. 18 page： 571-581 2009

Involvement of NbNOA1 in NO production and defense responses in INF1-treated Nicotiana benthamiana. Reviewed

Kato, H., Asai, S., Yamamoto-Katou, A., Yoshioka, H., Doke, N. and Kawakita, K.

Journal of Genaral Plant Pathology Vol. 74 page： 15-23 2008

Discovery of oxidative burst in the field of plant immunity: Looking back at the early pioneering works and towards the future development. Reviewed

Yoshioka, H., Bouteau, F. and Kawano, T.

Plant Signaling and Behavior Vol. 3 page： 153-155 2008

The role of radical burst via MAPK signaling in plant immunity. Invited Reviewed

Asai, S. and Yoshioka, H.

Plant Signaling and Behavior Vol. 3 page： 920-922 2008

*MAPK signaling regulates nitric oxide and NADPH oxidase-dependent oxidative bursts in Nicotiana benthamiana. Reviewed

Asai, S., Ohta, K. and Yoshioka, H.

The Plant Cell Vol. 20 page： 1390-1406 2008

*Calcium-dependent protein kinases regulate the production of reactive oxygen species by potato NADPH oxidase. Reviewed

Kobayashi, M., Ohura, I., Kawakita, K., Yokota, N., Fujiwara, M., Shimamoto, K., Doke, N. and Yoshioka, H.

The Plant Cell Vol. 19 page： 1065-1080 2007

Induction of a small heat shock protein and its functional roles in Nicotiana plants in the defense response against Ralstonia solanacearum. Reviewed

Maimbo, M., Ohnishi, K., Hikichi, Y., Yoshioka, H. and Kiba, A.

Plant Physiology Vol. 145 page： 1588-1599 2007

*Regulation of rice NADPH oxidase by binding of Rac GTPase to its N-terminal extension. Reviewed

Wong, H.L., Pinontoan, R., Hayashi, K., Tabata, R., Yaeno, T., Hasegawa, K., Kojima, C., Yoshioka, H., Iba, K., Kawasaki, T. and Shimamoto, K.

The Plant Cell Vol. 19 page： 4022-4034 2007

植物免疫におけるMAPキナーゼとラジカルバースト．植物における環境と生物ストレスに対する応答 Invited

吉岡博文・浅井秀太

蛋白質核酸酵素増刊号 Vol. 52 page： 667-672 2007

植物免疫におけるラジカルバーストの分子機構 Invited

吉岡博文・浅井秀太・間瀬圭介・市川達士・小林光智衣・小八重善裕

植物－病原微生物の相互作用のダイナミズム Vol. 43 page： 37-47 2007

植物病原細菌の感染を制御する宿主応答機構～ Ralstonia solanacearum- Nicotiana属植物相互作用の解析～ Invited

木場章範・Maimbo, M.・小森大輔・張　宛果・富山裕美・高橋彰子・大西浩平・吉岡博文・曳地康史

植物－病原微生物の相互作用のダイナミズム Vol. 43 page： 55-65 2007

Nitrate reductase is responsible for elicitin-induced nitric oxide production in Nicotiana benthamiana. Reviewed

Yamamoto-Katou, A., Katou, S., Yoshioka, H., Doke, N. and Kawakita, K.

Plant and Cell Physiology Vol. 47 page： 726-735 2006

Peroxynitrite generation and tyrosine nitration in defense responses in tobacco BY-2 cells.

Saito, S., Yamamoto-Katou, A., Yoshioka, H., Doke, N. and Kawakita, K.

Plant and Cell Physiology Vol. 47 page： 689-697 2006

The E3 ubiquitin ligase activity of Arabidopsis PLANT U-BOX17 and its functional tobacco homolog ACRE276 are required for cell death and defense. Reviewed

Yang, C.-W., Gonzalez-Lamothe, R., Ewan, R.A., Rowland, O., Yoshioka, H., Shenton, M., Ye, H., O'Donnell, E., Jones, J.D.G. and Sadanandom, A.

The Plant Cell Vol. 18 page： 1084-1098 2006

Subcellular localization of Strboh proteins and NADPH-dependent O₂^--generating activity in potato tuber tissues. Reviewed

Kobayashi, M., Kawakita, K., Maeshima, M., Doke, N. and Yoshioka, H.

Journal of Experimental Botany Vol. 57 page： 1373-1379 2006

Loss of AtPDR8, a plasma membrane ABC transporter of Arabidopsis thaliana, causes hypersensitive cell death upon pathogen infection. Reviewed

Kobae, Y., Sekino, T., Yoshioka, H., Nakagawa, T., Martinoia, E. and Maeshima, M.

Plant Cell Physiology Vol. 47 page： 309-318 2006

Rewiring mitogen-activated protein kinase cascade by positive feedback confers potato blight resistance. Reviewed

Yamamizo, C., Kuchimura, K., Kobayashi, A., Katou, S., Kawakita, K., Jones, J.D.G., Doke, N. and Yoshioka, H.

Plant Physiology Vol. 140 page： 681-692 2006

植物の感染応答因子としてのNO． Invited

川北一人・山本（加藤）文子・齋藤修平・加藤新平・加藤大明・浅井秀太・樹神博士・吉岡博文・道家紀志

病原体の寄生戦略と植物の応答 Vol. 42 page： 11-22 2006

Involvement of PPS3 phosphorylated by elicitor-responsive mitogen-activated protein kinases in the regulation of plant cell death. Reviewed

Katou, S., Yoshioka, H., Kawakita, K., Rowland, O., Jones, J.D.G., Mori, H. and Doke, N.

Plant Physiology Vol. 139 page： 1914-1926 2005

The Arabidopsis gene CAD1 controls programmed cell death in the plant immune system and encodes a protein containing a MACPF domain. Reviewed

Morita-Yamamuro, C., Tsutsui, T., Sato, M., Yoshioka, H., Tamaoki, M., Ogawa, D. Matsuura, H., Yoshihara, T., Ikeda, A., Uyeda, I. and Yamaguchi, J.

Plant and Cell Physiology Vol. 46 page： 902-912 2005.1

Functional analysis of Avr9/Cf-9 rapidly elicited genes identifies a protein kinase, ACIK1, that is essential for full Cf-9-dependent disease resistance in tomato. Reviewed

Rowland, O., Ludwig, A.A., Merrick, C.J., Baillieul, F., Tracy, F.E., Durrant, W.E., Fritz-Laylin, L., Nekrasov, V., Sjolander, K., Yoshioka, H. and Jones, J.D.G.

The Plant Cell Vol. 17 page： 295-310 2005.1

植物における活性酸素の役割とその誘導．活性酸素，－生産と防御のメカニズム－． Invited

吉岡博文・山溝千尋

蛋白質核酸酵素 Vol. 50 page： 310-315 2005

抵抗性遺伝子を介した防御応答におけるシグナル伝達機構． Invited

吉岡博文・山溝千尋・小林光智衣・水野貴仁・川北一人・道家紀志

植物・病原体相互反応における特異性決定機構 Vol. 41 page： 13-22 2005.8

Involvement of nitric oxide generation in hypersensitive cell death induced by elicitin in tobacco cell suspension culture.

Yamamoto, A., Katou, S., Yoshioka, H., Doke, N. and Kawakita, K.

Journal of Genaral Plant Pathology Vol. 70 page： 85-92 2004.1

Elicitation of primary and secondary metabolism during defense in the potato. Reviewed

Nakane, E., Kawakita, K., Doke, N. and Yoshioka, H.

Journal of Genaral Plant Pathology Vol. 69 page： 378-384 2003.1

Cytosolic HSP90 and HSP70 are essential components of INF1-mediated hypersensitive response and non-host resistance to Pseudomonas cichorii in Nicotiana benthamiana.

Kanzaki, H., Saitoh, H., Ito, A., Fujisawa, S., Kamoun, S., Katou, S., Yoshioka, H. and Terauchi, R.

Molecular Plant Pathology Vol. 4 page： 383-391 2003.1

Disease stress-inducible genes of tobacco: expression profile of elicitor-responsive genes isolated by substractive hybridization. Reviewed

Takemoto, D., Yoshioka, H., Doke, N. and Kawakita, K.

Physiologia Plantarum Vol. 118 page： 545-553 2003.1

Nitrate reductase, a nitric oxide-producing enzyme: induction by pathogen signals. Reviewed

Yamamoto, A., Katou, S., Yoshioka, H., Doke, N. and Kawakita, K.

Journal of Genaral Plant Pathology Vol. 69 page： 218-229 2003.1

Functional analysis of potato mitogen-activated protein kinase kinase, StMEK1. Reviewed

Katou, S., Yamamoto, A., Yoshioka. H., Kawakita, K. and Doke, N.

Journal of General Plant Pathology Vol. 69 page： 161-168 2003

*Nicotiana benthamiana gp91^phox homologs NbrbohA and NbrbohB participate in H₂O₂ accumulation and resistance to Phytophthora infestans. Reviewed

Yoshioka, H., Numata, N., Nakajima, K., Katou, S., Kawakita, K., Rowland, O., Jones, J.D.G. and Doke, N.

The Plant Cell Vol. 15 page： 706-718 2003.3

植物免疫としてのオキシダティブバーストを探る，－MAPKカスケードが活性酸素の生成と細胞死を制御する－． Invited

吉岡博文

化学と生物 Vol. 41 page： 639-641 2003

宿主植物の抵抗性制御と糸状菌病に対する耐病性強化の展望． Invited

吉岡博文・口村和男・山溝千尋・池田直希・加藤新平・川北一人・小林　晃・道家紀志

作物の耐病性強化戦略と植物－病原体相互作用の分子機構研究 Vol. 39 page： 101-110 2003

Induction of plant gp91 phox homolog by fungal cell wall, arachidonic acid, and salicylic acid in potato. Reviewed

Yoshioka, H., Sugie, K., Park, H.-J., Maeda, H., Tsuda, N., Kawakita, K. and Doke, N.

Molecular Plant-Microbe Interactions Vol. 14 page： 725-736 2001.1

cDNA cloning of sesquiterpene cyclase and squalene synthase, and expression of the genes in potato tuber infected with Phytophthora infestans. Reviewed

Yoshioka, H., Yamada, N. and Doke, N.

Plant and Cell Physiology Vol. 40 ( 9 ) page： 993-998 1999

Plasma membrane perturbation in association with calcium ion movement followed by fungal elicitor-stimulated oxidative burst and defense gene activation in potato tuber. Reviewed

Miura, Y., Yoshioka, H., Park, H.-J., Kawakita, K. and Doke, N.

Annals of the Phytopathological Society of Japan Vol. 65 page： 447-453 1999.1

A 51 kDa protein kinase of potato activated with hyphal wall components from Phytophthora infestans. Reviewed

Katou, S., Senda, K., Yoshioka, H., Doke, N. and Kawakita, K.

Plant and Cell Physiology Vol. 40 page： 825-831 1999.1

Effect of cytochalasin D on defense responses of potato tuber discs treated with hyphal wall components of Phytophthora infestans. Reviewed

Takemoto, D., Maeda, H., Yoshioka, H., Doke, N. and Kawakita, K.

Plant Science Vol. 141 page： 219-226 1999.1

Aspartate aminotransferase in alfalfa nodules: Localization of mRNA during effective and ineffective nodules development and promoter analysis. Reviewed

Yoshioka, H., Gregerson, R.G., Samac, D.A., Hoevens, K.C.M., Trepp, G., Gantt, J.S. and Vance, C.P.

Molecular Plant-Microbe Interactions Vol. 12 page： 263-274 1999.1

NADH-glutamate synthase in alfalfa root nodules: genetic regulation and cellular expression. Reviewed

Trepp, G., van de Mortel, M., Yoshioka, H., Miller, S.S., Samac, D.A., Gantt, J.S. and Vance, C.P.

Plant Physiology Vol. 119 page： 817-828 1999.1

誘導抵抗性関連研究の最前線． Invited

吉岡博文

日植病報バイオコントロール研究会レポート Vol. 6 page： 19-28 1999

Physiological mechanisms of a sub-systemic oxidative burst triggered by elicitor-induced local oxidative burst in potato tuber slices. Reviewed

Park, H.-J., Miura, Y., Kawakita, K., Yoshioka, H. and Doke, N.

Plant and Cell Physiology Vol. 39 ( 11 ) page： 1218-1225 1998

ジャガイモ塊茎における防御遺伝子発現とそのシグナル伝達． Invited

吉岡博文・伊藤夢子・杉江顕一・三浦由雄・川北一人・道家紀志

植物と病原体の情報応答機構 page： 49-108 1998

Nitrogen assimilation in alfalfa: Isolation and characterization of an asparagine synthetase gene showing enhanced expression in root nodules and dark-adapted leaves. Reviewed

Shi, L., Twary, S.N., Yoshioka, H., Gregerson, R.G., Miller, S.S., Samac, D.A., Gantt, J.S., Unkefer, P.J. and Vance, C.P.

The Plant Cell Vol. 9 page： 1339-1356 1997.1

Analyses of phosphoenolpyruvate carboxylase gene structure and expression in alfalfa nodules. Reviewed

Pathirana, S.M., Samac, D.A., Roeven, R., Yoshioka, H., Vance, C.P. and Gantt, J.S.

The Plant Journal Vol. 12 page： 293-304 1997.1

植物病原糸状菌のサプレッサーによる宿主防御遺伝子の発現制御機構に関する研究． Invited

吉岡博文

日本植物病理学会報 Vol. 63 page： 141 1997.1

植物の感染応答における情報伝達とホスホリパーゼ．植物感染生理学研究の現状と将来展望． Invited

川北一人・仙田香織・鈴木真紀・吉岡博文・道家紀志

植物感染生理学研究の現状と将来展望 Vol. 33 page： 134-142 1997

The oxidative burst protects plants against pathogen attack : mechanism and role as an emergency signal for plant bio-defence-a review. Invited Reviewed

Doke, N., Miura, Y., Sanchez, L.M., Park, H.-J., Noritake, T., Yoshioka, H. and Kawakita, K.

Gene Vol. 179 page： 45-51 1996

A cytosolic phospholipase A₂ from potato tissues appears to be patatin. Reviewed

Senda, K., Yoshioka, H., Doke, N. and Kawakita, K.

Plant and Cell Physiology Vol. 37 page： 347-353 1996.1

Expression of genes for phenylalanine ammonia-lyase and 3-hydroxy-3-methylglutaryl CoA reductase in aged potato tubers infected with Phytophthora infestans. Reviewed

Yoshioka, H., Miyabe, M., Hayakawa, Y. and Doke, N.

Plant and Cell Physiology Vol. 37 page： 81-90 1996.1

An autophotographic determination of the active oxygen generation in potato tuber discs during hypersensitive response to fungal infection or elicitor. Reviewed

Miura, Y., Yoshioka, H. and Doke, N.

Plant Science Vol. 105 page： 45-52 1995.1

Suppression of the activation of chitinase and β-1,3-glucanase in pea epicotyls by eogenous suppressor from Pisam sativum. Reviewed

Nasu, K., Yoshioka, H., Ichinose, Y., Yamada, T., Oku, H. and Shiraishi, T.

Annals of the Phytopathological Society of Japan Vol. 61 page： 13-17 1995.1

Suppression of phenylalanine ammonia-lyase mRNA accumulation by suppressors from Phytophthora infestans. Reviewed

Yoshioka, H., Hayakawa, Y. and Doke, N.

Annals of the Phytopathological Society of Japan Vol. 61 ( 1 ) page： 7-12 1995

Suppressors : Determinants of specificity produced by plant pathogens. Invited Reviewed

Shiraishi, T., Yamada, T., Saitoh, K., Kato, T., Toyoda, K., Yoshioka, H., Kim, H.-M., Ichinose, Y., Tahara, M. and Oku, H.

Plant and Cell Physiology Vol. 35 ( 8 ) page： 1107-1119 1994

Rapid accumulation of phenylalanine ammonia-lyase mRNA and 3-hydroxy-3-methylglutaryl CoA reductase mRNA by protein synthesis inhibitors. Reviewed

Yoshioka, H. and Doke, N.

Plant and Cell Physiology Vol. 35 ( 8 ) page： 1257-1260 1994

Race-cultivar specificity in plant fungal disease and its molecular mechanisms. Invited

Plant Cell Technology Vol. 5 ( 1 ) page： 19-27 1993

An endogenous suppressor of the defense response in Pisum sativum. Reviewed

Nasu, K., Shiraishi, T., Yoshioka, H., Ichinose, Y., Yamada, T. and Oku, H.

Plant and Cell Physiology Vol. 33 ( 5 ) page： 617-626 1992

Regulation of polyphosphoinositide metabolism in ea plasma membranes by elicitor and suppressor from a pea pathogen, Mycosphaerella pinodes. Reviewed

Toyoda, K., Shiraishi, T., Yoshioka, H., Yamada, T., Ichinose, Y. and Oku, H.

Plant and Cell Physiology Vol. 33 ( 4 ) page： 445-452 1992

Preparation of the infection court by Erysiphe graminis f. sp. hordei : Cutinase is a component of the conidial exudate. Reviewed

Pascholati, S.F., Yoshioka, H., Kunoh, H. and Nicholson, R.L.

Physiological and Molecular Plant Pathology Vol. 41 page： 53-59 1992

Suppression of the activation of chitinase and β-1,3-glucanase in pea epicotyls by orthonadate and a suppressor from Mycosphaerella pinodes. Reviewed

Yoshioka, H., Shiraishi, T., Yamada, T., Nasu, K., Ichinose, Y. and Oku, H.

Annals of the Phytopathological Society of Japan Vol. 58 ( 3 ) page： 405-410 1992

Orthovanadate suppresses accumulation of phenylalanine ammonia-lyase mRNA and chalcone synthase mRNA in pea epicotyls induced by elicitor from Mycosphaerella pinodes. Reviewed

Yoshioka, H., Shiraishi, T., Kawamata, S., Nasu, K., Yamada, T., Ichinose, Y. and Oku, H.

Plant and Cell Physiology Vol. 33 ( 2 ) page： 201-204 1992

Inhibition of ATPase activity in pea plasma membranes in situ by a suppressor from a pea pathogen, Mycosphaerella pinodes. Reviewed

Shiraishi, T., Araki, M., Yoshioka, H., Kobayashi, I., Yamada, T., Ichinose, Y., Kunoh, H. and Oku, H.

Plant and Cell Physiology Vol. 32 ( 7 ) page： 1067-1075 1991

エンドウ動的遺伝子の発現機構． Invited

山田哲治・白石友紀・一瀬勇規・奥　八郎・川又伸治・田中良和・Spirasertsak, P.・安　成才・吉岡博文・橋本忠明

生物相互間の認識機構 Vol. 27 page： 56-65 1991

Suppression of pisatin production and ATPase activity in pea plasma membranes by orthovanadate, verapamil and a suppressor from Mycosphaerella pinodes. Reviewed

Yoshioka, H., Shiraishi, T., Yamada, T., Ichinose, Y. and Oku, H.

Plant and Cell Physiology Vol. 31 ( 8 ) page： 1139-1146 1990

Preparation of the infection court by Erysiphe graminis : Degradation of the host cuticle. Reviewed

Kunoh, H., Nicholson, R.L., Yoshioka, H., Yamaoka, N. and Kobayashi, I.

Physiological and Molecular Plant Pathology Vol. 36 page： 397-407 1990

Preparation of the infection court by Erysiphe graminis. II. Release of esterase enzyme from conidia in response to a contact stimulus. Reviewed

Nicholson, R.L., Yoshioka, H., Yamaoka, N. and Kunoh, H.

Experimental Mycology Vol. 12 page： 336-349 1988

Preparation of the infection court by Erysiphe graminis. I. Contact-mediated changes in morphology of the conidium surface. Reviewed

Kunoh, H., Yamaoka, N., Yoshioka, H. and Nicholson, R.L.

Experimental Mycology Vol. 12 page： 325-335 1988

NADPH oxidase-mediated sulfenylation of cysteine derivatives regulates plant immunity

Hino, Y; Inada, T; Yoshioka, M; Yoshioka, H

JOURNAL OF EXPERIMENTAL BOTANY Vol. 75 ( 15 ) page： 4641 - 4654 2024.4

Dynamics of plant immune MAPK activity and ROS signaling in response to invaders

Hirofumi Yoshioka, Yuta Hino, Keiichiro Iwata, Takaya Ogawa, Miki Yoshioka, Nobuaki Ishihama, Hiroaki Adachi

Physiological and Molecular Plant Pathology Vol. 125 page： 102000 - 102000 2023.5

Nab-Paclitaxel for Previously Treated Advanced Non-Small Cell Lung Cancer: Analysis of Safety and Efficacy for Patients With Renal Impairment

Yoneshima, Y; Morita, S; Ando, M; Nakamura, A; Iwasawa, S; Yoshioka, H; Goto, Y; Takeshita, M; Harada, T; Hirano, K; Oguri, T; Kondo, M; Miura, S; Hosomi, Y; Kato, T; Kubo, T; Kishimoto, J; Yamamoto, N; Nakanishi, Y; Okamoto, I

CLINICAL LUNG CANCER Vol. 23 ( 7 ) page： 585 - 592 2022.11

Development of plant activator loaded nano-particles Reviewed

Yamaguchi Kento, Kitagawa Takahiro, Tobinaga Kyouhei, Saito Takao, Yoshioka Hirofumi, Narusaka Yoshihiro

Japanese Journal of Pesticide Science Vol. 47 ( 2 ) page： 65 - 68 2022.8

Multicentre, prospective, observational study investigating the most appropriate surgical option that can prevent the recurrence of primary spontaneous pneumothorax after surgery: the PATCH study, protocol

Kadomatsu, Y; Yoshioka, H; Shigemitsu, K; Nomata, Y; Mori, S; Hijiya, K; Motoyama, H; Ichikawa, Y; Sueyoshi, K; Okasaka, T; Miyamoto, E; Kobayashi, M; Takahashi, M; Fujinaga, T; Takechi, H; Yamagishi, H; Takuwa, T; Kobayashi, J; Sakamoto, J; Taniguchi, T; Hanaoka, N; Kubo, Y; Chen-Yoshikawa, TF

BMJ OPEN Vol. 11 ( 12 ) page： e052045 2021.12

Senescent cell removal via JAG1-NOTCH1 signalling in the epidermis

Yoshioka, H; Yamada, T; Hasegawa, S; Miyachi, K; Ishii, Y; Hasebe, Y; Inoue, Y; Tanaka, H; Iwata, Y; Arima, M; Sugiura, K; Akamatsu, H

EXPERIMENTAL DERMATOLOGY Vol. 30 ( 9 ) page： 1268 - 1278 2021.9

Prognostic impact of geriatric assessment in elderly patients with non-small cell lung cancer: an integrated analysis of two randomized phase III trials (JCOG1115-A)

Katayama, H; Mizusawa, J; Fukuda, H; Nakamura, S; Nakamura, K; Saijo, N; Yokoyama, A; Ohe, Y; Shinkai, T; Nakagawa, K; Abe, T; Mitsuoka, S; Okamoto, H; Yamamoto, N; Yoshioka, H; Ando, M; Tamura, T; Takeda, K

JAPANESE JOURNAL OF CLINICAL ONCOLOGY Vol. 51 ( 5 ) page： 685 - 692 2021.5

Oral RNAi ofdiap1results in rapid reduction of damage to potatoes inHenosepilachna vigintioctopunctata

Chikami, Y; Kawaguchi, H; Suzuki, T; Yoshioka, H; Sato, Y; Yaginuma, T; Niimi, T

JOURNAL OF PEST SCIENCE Vol. 94 ( 2 ) page： 505 - 515 2021.3

Phase III Study Comparing Nab-Paclitaxel With Docetaxel in Patients With Previously Treated Advanced Non-Small-Cell Lung Cancer

Nakamura, A; Yoneshima, Y; Morita, S; Ando, M; Iwasawa, S; Yoshioka, H; Goto, Y; Takeshita, M; Harada, T; Hirano, K; Oguri, T; Kondo, M; Miura, S; Hosomi, Y; Kato, T; Kubo, T; Kishimoto, J; Yamamoto, N; Nakanishi, Y; Okamoto, I

JOURNAL OF THORACIC ONCOLOGY Vol. 16 ( 3 ) page： S107 - S108 2021.3

Role of salicylic acid glucosyltransferase in balancing growth and defence for optimum plant fitness. Reviewed International journal

Yudai Kobayashi, Noriho Fukuzawa, Ayaka Hyodo, Hangil Kim, Shota Mashiyama, Tsuyoshi Ogihara, Hirofumi Yoshioka, Hideyuki Matsuura, Chikara Masuta, Takeshi Matsumura, Minoru Takeshita

Molecular plant pathology Vol. 21 ( 3 ) page： 429 - 442 2020.3

Language：English Publishing type：Research paper (scientific journal)

Salicylic acid (SA), an essential secondary messenger for plant defence responses, plays a role in maintaining a balance (trade-off) between plant growth and resistance induction, but the detailed mechanism has not been explored. Because the SA mimic benzothiadiazole (BTH) is a more stable inducer of plant defence than SA after exogenous application, we analysed expression profiles of defence genes after BTH treatment to better understand SA-mediated immune induction. Transcript levels of the salicylic acid glucosyltransferase (SAGT) gene were significantly lower in BTH-treated Nicotiana tabacum (Nt) plants than in SA-treated Nt control plants, suggesting that SAGT may play an important role in SA-related host defence responses. Treatment with BTH followed by SA suppressed SAGT transcription, indicating that the inhibitory effect of BTH is not reversible. In addition, in BTH-treated Nt and Nicotiana benthamiana (Nb) plants, an early high accumulation of SA and SA 2-O-β-d-glucoside was only transient compared to the control. This observation agreed well with the finding that SAGT-overexpressing (OE) Nb lines contained less SA and jasmonic acid (JA) than in the Nb plants. When inoculated with a virus, the OE Nb plants showed more severe symptoms and accumulated higher levels of virus, while resistance increased in SAGT-silenced (IR) Nb plants. In addition, the IR plants restricted bacterial spread to the inoculated leaves. After the BTH treatment, OE Nb plants were slightly larger than the Nb plants. These results together indicate that SAGT has a pivotal role in the balance between plant growth and SA/JA-mediated defence for optimum plant fitness.

DOI： 10.1111/mpp.12906

Web of Science

PubMed

植物を元気にして病気を防ぐ―植物活力剤によるイチゴの病害抑制技術― Invited

鳴坂義弘, 鳴坂真理, 吉岡博文, 吉岡美樹, 谷口伸治, 石川美友紀, 紀岡雄三, 野口勝憲

植物防疫 Vol. 73 ( 11 ) page： 684-688 2019.11

Ph3 Study of Maintenance Therapy with S-1 vs BSC After Induction Therapy with Carboplatin + S-1 for Advanced Squamous Cell Lung Cancer (WJOG7512L)

Tanaka, K; Morita, S; Ando, M; Yokoyama, T; Nakamura, A; Yoshioka, H; Ishiguro, T; Miura, S; Toyozawa, R; Oguri, T; Daga, H; Ko, R; Bessho, A; Tachihara, M; Iwamoto, Y; Hirano, K; Nakanishi, Y; Nakagawa, K; Yamamoto, N; Okamoto, I

JOURNAL OF THORACIC ONCOLOGY Vol. 14 ( 10 ) page： S302 - S302 2019.10

Randomized phase 3 study of maintenance therapy with S-1 plus best supportive care (BSC) versus BSC alone after induction therapy with carboplatin plus S-1 for advanced or relapsed squamous cell lung carcinoma (WJOG7512L).

JOURNAL OF CLINICAL ONCOLOGY Vol. 37 ( 15 ) 2019.5

WRKYs phosphorylated by MAPK regulate chloroplast-mediated ROS burst in plant immunity

Yoshioka, H; Adachi, H; Yoshioka, M

PHYTOPATHOLOGY Vol. 108 ( 10 ) 2018.10

Phase Competition, Solitons, and Domain Walls in Neutral-Ionic Transition Systems

Tsuchiizu, M; Yoshioka, H; Seo, H

JOURNAL OF THE PHYSICAL SOCIETY OF JAPAN Vol. 85 ( 10 ) 2016.10

*WRKY transcription factors phosphorylated by MAPK regulate a plant immune NADPH oxidase in Nicotiana benthamiana. Reviewed

Adachi, H, Nakano, T, Miyagawa, N, Ishihama, N, Yoshioka, M, Katou, Y, Yaeno, T, Shirasu, K, Yoshioka, H

The Plant Cell Vol. 27 ( 9 ) page： 2645-2663. 2015.10

リン酸化反応が制御するROSバーストの分子機構 Invited Reviewed

吉岡博文, 安達広明, 石濱伸明, 中野孝明, 白石佑太郎, 宮川典子, 野村裕也, 吉岡美樹, 浅井秀太

日本植物病理学会報 Vol. 81 page： 1-8. 2015.2

SEC14 Phospholipid Transfer Protein Is Involved in Lipid Signaling-Mediated Plant Immune Responses in Nicotiana benthamiana Reviewed

Akinori Kiba, Ivan Galis, Yuko Hojo, Kouhei Ohnishi, Hirofumi Yoshioka, Yasufumi Hikichi

PLOS ONE Vol. 9 ( 5 ) page： e98150 2014.5

Language：English Publishing type：Research paper (scientific journal) Publisher：PUBLIC LIBRARY SCIENCE

We previously identified a gene related to the SEC14-gene phospholipid transfer protein superfamily that is induced in Nicotiana benthamiana (NbSEC14) in response to infection with Ralstonia solanacearum. We here report that NbSEC14 plays a role in plant immune responses via phospholipid-turnover. NbSEC14-silencing compromised expression of defense-related PR-4 and accumulation of jasmonic acid (JA) and its derivative JA-Ile. Transient expression of NbSEC14 induced PR-4 gene expression. Activities of diacylglycerol kinase, phospholipase C and D, and the synthesis of diacylglycerol and phosphatidic acid elicited by avirulent R. solanacearum were reduced in NbSEC14-silenced plants. Accumulation of signaling lipids and activation of diacylglycerol kinase and phospholipases were enhanced by transient expression of NbSEC14. These results suggest that the NbSEC14 protein plays a role at the interface between lipid signaling-metabolism and plant innate immune responses.

DOI： 10.1371/journal.pone.0098150

Web of Science

PubMed

In Vivo Phosphorylation of WRKY Transcription Factor by MAPK

Nobuaki Ishihama, Hiroaki Adachi, Miki Yoshioka, Hirofumi Yoshioka

Methods in Molecular Biology Vol. 1171 page： 171 - 181 2014

Suppression of DS1 Phosphatidic Acid Phosphatase Confirms Resistance to Ralstonia solanacearum in Nicotiana benthamiana Reviewed

Masahito Nakano, Masahiro Nishihara, Hirofumi Yoshioka, Hirotaka Takahashi, Tatsuya Sawasaki, Kouhei Ohnishi, Yasufumi Hikichi, Akinori Kiba

PLOS ONE Vol. 8 ( 9 ) page： e75124. 2013.9

Language：English Publishing type：Research paper (scientific journal) Publisher：PUBLIC LIBRARY SCIENCE

Nicotiana benthamiana is susceptible to Ralstonia solanacearum. To analyze molecular mechanisms for disease susceptibility, we screened a gene-silenced plant showing resistance to R. solanacearum, designated as DS1 (Disease suppression 1). The deduced amino acid sequence of DS1 cDNA encoded a phosphatidic acid phosphatase (PAP) 2. DS1 expression was induced by infection with a virulent strain of R. solanacearum in an hrp-gene-dependent manner. DS1 rescued growth defects of the temperature-sensitive Delta lpp1 Delta dpp1 Delta pah1 mutant yeast. Recombinant DS1 protein showed Mg2+-independent PAP activity. DS1 plants showed reduced PAP activity and increased phosphatidic acid (PA) content. After inoculation with R. solanacearum, DS1 plants showed accelerated cell death, over-accumulation of reactive oxygen species (ROS), and hyper-induction of PR-4 expression. In contrast, DS1-overexpressing tobacco plants showed reduced PA content, greater susceptibility to R. solanacearum, and reduced ROS production and PR-4 expression. The DS1 phenotype was partially compromised in the plants in which both DS1 and NbCoi1 or DS1 and NbrbohB were silenced. These results show that DS1 PAP may affect plant immune responses related to ROS and JA cascades via regulation of PA levels. Suppression of DS1 function or DS1 expression could rapidly activate plant defenses to achieve effective resistance against Ralstonia solanacearum.

DOI： 10.1371/journal.pone.0075124

Web of Science

PubMed

A translationally controlled tumor protein negatively regulates the hypersensitive response in Nicotiana benthamiana. Reviewed

Gupta, M, Yoshioka, H, Ohnishi, K, Mizumoto, H, Hikichi, Y, Kiba, A

Plant and Cell Physiology Vol. 54 ( 8 ) page： 1403-1414. 2013.8

タンパク質のリン酸化によるROSバーストの分子機構 Invited

吉岡博文, 安達広明, 石濱伸明, 中野孝明, 白石佑太郎, 宮川典子, 野村裕也, 吉岡美樹, 浅井秀太

植物−微生物相互作用とビオトロフ感染 Vol. 48 page： 105-114. 2013.8

Ethylene responsive AP2/ERF transcription factor MACD1 participates in phytotoxin-triggered programmed cell death. Reviewed

Mase, K, Ishihama, N, Mori, H, Takahashi, H, Kaminaka, H, Kodama, M, Yoshioka, H

Molecular Plant-Microbe Interactions Vol. 26 page： 868-879. 2013.7

*The variable domain of a plant calcium-dependent protein kinase (CDPK) confers subcellular localization and substrate recognition for NADPH oxidase. Reviewed

Asai, S, Ichikawa, T, Nomura, H, Kobayashi, M, Kamiyoshihara, Y, Mori, H, Kadota, Y, Zipfel, C, Jones, J.D.G, Yoshioka, H

The Journal of Biological Chemistry Vol. 288 page： 14332-14340. 2013.5

The Variable Domain of a Plant Calcium-dependent Protein Kinase (CDPK) Confers Subcellular Localization and Substrate Recognition for NADPH Oxidase

Asai, S; Ichikawa, T; Nomura, H; Kobayashi, M; Kamiyoshihara, Y; Mori, H; Kadota, Y; Zipfel, C; Jones, JDG; Yoshioka, H

JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 288 ( 20 ) page： 14332 - 14340 2013.5

Nitric Oxide as a Mediator for Defense Responses

Bellin, D; Asai, S; Delledonne, M; Yoshioka, H

MOLECULAR PLANT-MICROBE INTERACTIONS Vol. 26 ( 3 ) page： 271 - 277 2013.3

*StCDPK5 confers resistance to late blight pathogen but increases susceptibility to early blight pathogen in potato via reactive oxygen species burst. Reviewed

Kobayashi, M, Yoshioka, M, Asai, S, Nomura, H, Kuchimura, K, Mori, H, Doke, N, Yoshioka, H

New Phytologist Vol. 196 page： 223–237. 2012.10

StCDPK5 confers resistance to late blight pathogen but increases susceptibility to early blight pathogen in potato via reactive oxygen species burst

Kobayashi, M; Yoshioka, M; Asai, S; Nomura, H; Kuchimura, K; Mori, H; Doke, N; Yoshioka, H

NEW PHYTOLOGIST Vol. 196 ( 1 ) page： 223 - 237 2012.10

Ethylene Signaling Pathway and MAPK Cascades Are Required for AAL Toxin-Induced Programmed Cell Death

Mase, K; Mizuno, T; Ishihama, N; Fujii, T; Mori, H; Kodama, M; Yoshioka, H

MOLECULAR PLANT-MICROBE INTERACTIONS Vol. 25 ( 8 ) page： 1015 - 1025 2012.8

Post-translational regulation of WRKY transcription factors in plant immunity Invited Reviewed

Nobuaki Ishihama, Hirofumi Yoshioka

CURRENT OPINION IN PLANT BIOLOGY Vol. 15 ( 4 ) page： 431 - 437 2012.8

Language：English Publishing type：Research paper (scientific journal) Publisher：CURRENT BIOLOGY LTD

Plants have evolved immune system to protect themselves against invading pathogens. Recent research has illustrated that signaling networks, after perception of diverse pathogen-derived signals, facilitate transcriptional reprogramming through mitogen-activated protein kinase (MAPK) cascades. WRKY proteins, which comprise a large family of plant transcription factors, are key players in plant immune responses. WRKY transcription factors participate in the control of defense-related genes either as positive or as negative regulators, and essentially are regulated at the transcriptional level. Emerging evidence emphasizes that group I WRKY transcription factors, which contain a conserved motif in the N-terminal region, are also activated by MAPK-dependent phosphorylation, underlining their importance in plant immunity.

DOI： 10.1016/j.pbi.2012.02.003

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PubMed

Cell Death of Nicotiana benthamiana Is Induced by Secreted Protein NIS1 of Colletotrichum orbiculare and Is Suppressed by a Homologue of CgDN3

Yoshino, K; Irieda, H; Sugimoto, F; Yoshioka, H; Okuno, T; Takano, Y

MOLECULAR PLANT-MICROBE INTERACTIONS Vol. 25 ( 5 ) page： 625 - 636 2012.5

Observation of Riemannian geometric effects on electronic states

Onoe, J; Ito, T; Shima, H; Yoshioka, H; Kimura, S

EPL Vol. 98 ( 2 ) 2012.4

Isolation and characterization of an asparagine-rich protein that regulates hypersensitive cell death-mediated resistance in Nicotiana plants Reviewed

Daisuke Komori, Masahiro Nishihara, Akiko Takahashi, Meenu Gupta, Hirofumi Yoshioka, Hiroyuki Mizumoto, Kouhei Ohnishi, Yusufumi Hikichi, Akinori Kiba

PLANT BIOTECHNOLOGY Vol. 29 ( 3 ) page： 293 - 300 2012

Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOC PLANT CELL & MOLECULAR BIOLOGY

We isolated and characterized Ralstonia solanacearum-responsive genes (RsRGs) related to the hypersensitive response (HR) by virus-induced gene silencing using Nicotiana benthamiana and the potato virus x vector system. We focused on RsRGM10, because induction of HR was delayed in RsRGM10-silenced plants challenged with incompatible R. solanacearum 8107, which induces the HR in, N. benthamiana. The amino acid sequence deduced from the full-length RsRGM10 cDNA showed approximately 90% identity with rolB overexpressed 1 (ROX1) from N. tabacum, and contained an asparagine-rich sequence. We designated this protein NbARP (N. benthamiana asparagine-rich protein). Expression of HR-related hin1 and onset of the oxidative burst were delayed in NbARP-silenced plants, and induction of myelin basic kinase activity was reduced. Growth of R. solanacearum 8107 was accelerated in NbARP-silenced plants. Induction of the HR by Pseudomonas cichorii and Pseudomonas syringae pv. syringae was also delayed in NbARP-silenced plants. Silencing of NbARP reduced induction of HR-cell death by Agrobacterium tumefaciens-mediated transient expression of HR elicitors and a constitutively active form of mitogen activated protein kinase kinase. The population of R. solanacearum 8107 was decreased in NbARP-overexpressing plants. These results suggest that NbARP is closely related to the HR.

DOI： 10.5511/plantbiotechnology.12.0213b

Web of Science

SHAPE OPTIMIZATION OF THERMOELASTIC FIELDS FOR STIFFNESS MAXIMIZATION

Katamine, E; Yoshioka, H; Azegami, H

PROCEEDINGS OF THE ASME INTERNATIONAL DESIGN ENGINEERING TECHNICAL CONFERENCES AND COMPUTERS AND INFORMATION IN ENGINEERING CONFERENCE 2012, VOL 2, PTS A AND B page： 853 - + 2012

NINJAはコリプレッサーTOPLESSとジャスモン酸シグナルをつなぐ―植物ホルモンの受容による新しい転写制御機構の役割は？ Invited

野村裕也, 吉岡博文

化学と生物 Vol. 49 page： 85-87 2011.2

Nitric oxide and reactive oxygen species in plant defense responses Invited

Yoshioka H, Asai S, Yoshioka M, Kobayashi M, Doke N

In Genome-Enabled Integration of Research in Plant Pathogen Systems. (Wolpert, T., Shiraishi, T., Allen, C., Glazebrook, J. and Akimitsu, K. eds), APS Press page： 47 - 55 2011

Analyses of the cis-Regulatory Regions Responsible for the Transcriptional Activation of the N Resistance Gene by Tobacco mosaic virus Reviewed

Michie Kobayashi, Nobuaki Ishihama, Hirofumi Yoshioka, Reona Takabatake, Shinya Tsuda, Shigemi Seo, Yuko Ohashi, Ichiro Mitsuhara

JOURNAL OF PHYTOPATHOLOGY Vol. 158 ( 11-12 ) page： 826 - 828 2010.12

Language：English Publishing type：Research paper (scientific journal) Publisher：WILEY-BLACKWELL

Although the tobacco N gene, which confers resistance to Tobacco mosaic virus (TMV), is transcriptionally induced by infection with TMV, the regulatory mechanism has not previously been elucidated. We found that a 5'-flanking region of the N gene exhibits TMV-inducible promoter activity and that the region from -290 to -271 includes a cis-acting element in response to infection. The activity required N protein and signal components of N-mediated resistance. These results suggest that transcription of the N gene is positively regulated by N-mediated resistance signalling.

DOI： 10.1111/j.1439-0434.2010.01686.x

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Regulation of Arabidopsis defense responses against Spodoptera littoralis by CPK-mediated calcium signaling Reviewed

Chidananda Nagamangala Kanchiswamy, Hirotaka Takahashi, Stefano Quadro, Massimo E. Maffei, Simone Bossi, Cinzia Bertea, Simon Atsbaha Zebelo, Atsushi Muroi, Nobuaki Ishihama, Hirofumi Yoshioka, Wilhelm Boland, Junji Takabayashi, Yaeta Endo, Tatsuya Sawasaki, Gen-ichiro Arimura

BMC PLANT BIOLOGY Vol. 10 page： 97 2010.5

Language：English Publishing type：Research paper (scientific journal) Publisher：BIOMED CENTRAL LTD

Background: Plant Ca(2+) signals are involved in a wide array of intracellular signalling pathways after pest invasion. Ca(2+)-binding sensory proteins such as Ca(2+)-dependent protein kinases (CPKs) have been predicted to mediate the signaling following Ca(2+) influx after insect herbivory. However, until now this prediction was not testable.
Results: To investigate the roles CPKs play in a herbivore response-signaling pathway, we screened the characteristics of Arabidopsis CPK mutants damaged by a feeding generalist herbivore, Spodoptera littoralis. Following insect attack, the cpk3 and cpk13 mutants showed lower transcript levels of plant defensin gene PDF1.2 compared to wild-type plants. The CPK cascade was not directly linked to the herbivory-induced signaling pathways that were mediated by defense-related phytohormones such as jasmonic acid and ethylene. CPK3 was also suggested to be involved in a negative feedback regulation of the cytosolic Ca(2+) levels after herbivory and wounding damage. In vitro kinase assays of CPK3 protein with a suite of substrates demonstrated that the protein phosphorylates transcription factors (including ERF1, HsfB2a and CZF1/ZFAR1) in the presence of Ca(2+). CPK13 strongly phosphorylated only HsfB2a, irrespective of the presence of Ca(2+). Furthermore, in vivo agroinfiltration assays showed that CPK3-or CPK13-derived phosphorylation of a heat shock factor (HsfB2a) promotes PDF1.2 transcriptional activation in the defense response.
Conclusions: These results reveal the involvement of two Arabidopsis CPKs (CPK3 and CPK13) in the herbivory-induced signaling network via HsfB2a-mediated regulation of the defense-related transcriptional machinery. This cascade is not involved in the phytohormone-related signaling pathways, but rather directly impacts transcription factors for defense responses.

DOI： 10.1186/1471-2229-10-97

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PubMed

S-Glycoprotein-Like Protein Regulates Defense Responses in Nicotiana Plants against Ralstonia solanacearum Reviewed

Milimo Maimbo, Kouhei Ohnishi, Yasufumi Hikichi, Hirofumi Yoshioka, Akinori Kiba

PLANT PHYSIOLOGY Vol. 152 ( 4 ) page： 2023 - 2035 2010.4

Language：English Publishing type：Research paper (scientific journal) Publisher：AMER SOC PLANT BIOLOGISTS

RsRGA4 (for Ralstonia solanacearum-responsive gene A4) encodes a polypeptide similar to S-locus glycoprotein (SGP) from Brassica rapa and SGP-like proteins from Ipomoea trifida and Medicago truncatula. Therefore, we designated RsRGA4 as NtSGLP (for Nicotiana tabacum SGP-like protein) and NbSGLP (its Nicotiana benthamiana ortholog). NbSGLP is expressed in root, leaf, petal, gynoecium, and stamen. Expression of NbSGLP was strongly induced by inoculation with an avirulent strain of R. solanacearum (Rs8107) and slightly enhanced by inoculation with virulent R. solanacearum (RsOE1-1). Expression of NbSGLP was induced by inoculation with an hrpY-deficient mutant of RsOE1-1 and Rs8107. Expression was also induced by aminocyclopropane carboxylic acid and salicylic acid. Virus-induced gene silencing of NbSGLP enhanced the growth of Rs8107. Growth of RsOE1-1 and appearance of wilt symptoms were also accelerated in silenced plants. Expression of PR-1a and EREBP was reduced, and markers for basal defense, such as callose deposition and reduced vascular flow, were compromised in NbSGLP-silenced plants. Moreover, growth of Pseudomonas cichorii, Pseudomonas syringae pv tabaci, and P. syringae pv mellea was also enhanced in the silenced plants. On the other hand, silencing of NbSGLP did not interfere with the appearance of the hypersensitive response. NbSGLP was secreted in a signal peptide-dependent manner. Agrobacterium tumefaciens-mediated expression of NbSGLP induced PR-1a and EREBP expression, callose deposition, and reduced vascular flow. NbSGLP-induced callose deposition and reduced vascular flow were not observed in salicylic acid-deficient N. benthamiana NahG plants. Taken together, SGLP might have a role in the induction of basal defense in Nicotiana plants.

DOI： 10.1104/pp.109.148189

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PubMed

Ca2+-dependent protein kinases and their substrate HsfB2a are differently involved in the heat response signaling pathway in Arabidopsis Reviewed

Chidananda Nagamangala Kanchiswamy, Atsushi Muroi, Massimo E. Maffei, Hirofumi Yoshioka, Tatsuya Sawasaki, Gen-ichiro Arimura

PLANT BIOTECHNOLOGY Vol. 27 ( 5 ) page： 469 - 473 2010

Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOC PLANT CELL & MOLECULAR BIOL

Little is known about the mechanisms by which Ca2+-binding sensory proteins direct the plant heat shock (HS) response. Since two Ca2+-dependent protein kinases (CPK3 and CPK13) were recently shown to phosphorylate the heat shock transcription factor HsfB2a, we assessed in the current study whether these kinases are also involved in HS signal transduction, by monitoring the transcriptional profile of HS protein (Hsp) family genes in Arabidopsis Col-0 plants (WT) and the corresponding mutants. Both with and without HS, the gene transcript levels of Hsp70, Hsp101, Hsp17.4-CIII and Hsp15.7-CI were found to be lower in cpk3 and cpk13 mutants compared to WT, resulting in the impairment of basal thermotolerance in the mutants. To determine the in vivo function of CPKs, CPK3/13 and their substrate HsfB2a (heat shock transcription factor) were co-expressed as cofactors for the transient expression of a reporter (GUS) gene under the control of heat shock element (HSE) in Nicotiana benthamiana leaves. However, CPK3/13-phosphorylated HsfB2a did not function in the suppression/activation of HSE-promoted expression in the transient expression system. Implications for possible signal trafficking via CPKs and Hsfs are discussed.

DOI： 10.5511/plantbiotechnology.10.0728b

Web of Science

Remotely operable compact instruments for measuring atmospheric CO2 and CH4 column densities at surface monitoring sites

Kobayashi, N; Inoue, G; Kawasaki, M; Yoshioka, H; Minomura, M; Murata, I; Nagahama, T; Matsumi, Y; Tanaka, T; Morino, I; Ibuki, T

ATMOSPHERIC MEASUREMENT TECHNIQUES Vol. 3 ( 4 ) page： 1103 - 1112 2010

病害抵抗性・感受性決定に関わる植物の感染応答機構 Invited

木場章範, 中野真人, 伊藤慎, Maimbo, M, 大西浩平, 吉岡博文, 水本祐之, 曵地康史

農業現場の問題解決に向けた感染生理学 Vol. 46 page： 1-8 2010

Role of nitric oxide and reactive oxygen species in disease resistance to necrotrophic pathogens. Invited Reviewed

Asai, S, Mase, K, Yoshioka, H

Plant Signaling and Behavior Vol. 5 page： 872-874 2010

Riboflavin synthesis participates in plant immune responses in Nicotiana benthamiana Invited

Asai S, Mase K, Yoshioka H

In Biology of Plant-Microbe Interactions Vol. 7. Chapter 28, Proceedings of the 14th International Congress on Molecular Plant-Microbe Interactions. (Antoun, H., Avis, T., Brisson, L., Prevost, D. and Trepanier, M. eds), IS-MPMI Press, MN 2010

Molecular mechanisms of generation for nitric oxide and reactive oxygen species, and role of the radical burst in plant immunity Invited Reviewed

Hirofumi Yoshioka, Shuta Asai, Miki Yoshioka, Michie Kobayashi

MOLECULES AND CELLS Vol. 28 ( 4 ) page： 321 - 329 2009.10

Language：English Publishing type：Research paper (scientific journal) Publisher：KOREAN SOC MOLECULAR & CELLULAR BIOLOGY

Rapid production of nitric oxide (NO) and reactive oxygen species (ROS) has been implicated in the regulation of innate immunity in plants. A potato calcium-dependent protein kinase (StCDPK5) activates an NADPH oxidase StRBOHA to D by direct phosphorylation of N-terminal regions, and heterologous expression of StCDPK5 and StRBOHs in Nicotiana benthamiana results in oxidative burst. The transgenic potato plants that carry a constitutively active StCDPK5 driven by a pathogen-inducible promoter of the potato showed high resistance to late blight pathogen Phytophthora infestans accompanied by HR-like cell death and H(2)O(2) accumulation in the attacked cells. In contrast, these plants showed high susceptibility to early blight necrotrophic pathogen Alternaria solani, suggesting that oxidative burst confers high resistance to biotrophic pathogen, but high susceptibility to necrotrophic pathogen. NO and ROS synergistically function in defense responses. Two MAPK cascades, MEK2-SIPK and cytokinesis-related MEK1-NTF6, are involved in the induction of NbRBOHB gene in N. benthamiana. On the other hand, NO burst is regulated by the MEK2-SIPK cascade. Conditional activation of SIPK in potato plants induces oxidative and NO bursts, and confers resistance to both biotrophic and necrotrophic pathogens, indicating the plants may have obtained during evolution the signaling pathway which regulates both NO and ROS production to adapt to wide-spectrum pathogens.

DOI： 10.1007/s10059-009-0156-2

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INF1 Elicitin Activates Jasmonic Acid- and Ethylene-mediated Signalling Pathways and Induces Resistance to Bacterial Wilt Disease in Tomato Reviewed

Yoko Kawamura, Shu Hase, Shigehito Takenaka, Yoshinori Kanayama, Hirofumi Yoshioka, Sophien Kamoun, Hideki Takahashi

JOURNAL OF PHYTOPATHOLOGY Vol. 157 ( 5 ) page： 287 - 297 2009.5

Language：English Publishing type：Research paper (scientific journal) Publisher：WILEY-BLACKWELL PUBLISHING, INC

Phytophthora infestans, the cause of potato and tomato late blight disease, produces INF1 elicitin, a 10 kDa extracellular protein. INF1 induces a hypersensitive response (HR) and systemic acquired resistance in species of the Nicotiana genus and a few other genera. We analysed the response of tomato to INF1 and INF1S3, which has a Cys to Ser substitution at position 3 of the processed protein and therefore lacks HR induction activity in tobacco. No HR cell death was induced in either INF1- or INF1S3-treated tomato leaves. The expression of salicylic acid (SA)-responsive PR-1a(P6) and PR-2a genes was not induced by treatment with either INF1 or INF1S3. However, the expression of jasmonic acid (JA)-responsive PR-6 encoding proteinase inhibitor II, LeATL6 encoding ubiquitin ligase E3, and LOX-E encoding lipoxygenase, was up-regulated in tomato leaves treated with INF1 but not in those treated with INF1S3. Their induction was completely compromised in INF1-treated jai1-1 mutant tomato, in which the JA signalling pathway is impaired. The accumulation of ethylene (ET) and the expression of ET-responsive genes were also induced in tomato by INF1 but not INF1S3 treatment. The activation of JA and ET-mediated signals but not the SA-mediated signalling in INF1-treated tomato was also demonstrated by global gene expression analysis. INF1-treated tomatoes, but not those treated with INF1S3, exhibited resistance to bacterial wilt disease caused by Ralstonia solanacearum. Thus, INF1 seems to induce resistance to bacterial wilt disease in tomato and activate JA- and ET-mediated signalling pathways without development of HR cell death.

DOI： 10.1111/j.1439-0434.2008.01489.x

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Molecular mechanisms of the radical burst in plant immunity Invited

Yoshioka H, Asai S, Miyagawa N, Ichikawa T, Yoshioka M, Kobayashi M

In Molecular Plant-Microbe Interactions. (Bouarab, K., Brisson, N. and Daayf, F. eds), CAB International, Oxfordshire, UK page： 59 - 74 2009

Nitric oxide as a partner of reactive oxygen species participates in disease resistance to necrotrophic pathogen Botrytis cinerea in Nicotiana benthamiana. Reviewed

Asai, S, Yoshioka, H

Molecular Plant-Microbe Interactions Vol. 22 page： 619-629 2009

Molecular Mechanisms of the Radical Burst in Plant Immunity

Yoshioka, H; Asai, S; Miyagawa, N; Ichikawa, T; Yoshioka, M; Kobayashi, M

MOLECULAR PLANT-MICROBE INTERACTIONS page： 59 - 74 2009

MAPK signaling regulates nitric oxide and NADPH oxidase-dependent oxidative bursts in Nicotiana benthamiana

Asai, S; Ohta, K; Yoshioka, H

PLANT CELL Vol. 20 ( 5 ) page： 1390 - 1406 2008.5

Involvement of NbNOA1 in NO production and defense responses in INF1-treated Nicotiana benthamiana

Kato, H; Asai, S; Yamamoto-Katou, A; Yoshioka, H; Doke, N; Kawakita, K

JOURNAL OF GENERAL PLANT PATHOLOGY Vol. 74 ( 1 ) page： 15 - 23 2008.2

*MAPK signaling regulates nitric oxide and NADPH oxidase-dependent oxidative bursts in Nicotiana benthamiana. Reviewed

Asai, S, Ohta, K, Yoshioka, H

The Plant Cell Vol. 20 page： 1390-1406 2008

Discovery of oxidative burst in the field of plant immunity: Looking back at the early pioneering works and towards the future development. Reviewed

Yoshioka, H, Bouteau, F, Kawano, T

Plant Signaling and Behavior Vol. 3 page： 153-155 2008

The role of radical burst via MAPK signaling in plant immunity. Invited Reviewed

Asai, S, Yoshioka, H

Plant Signaling and Behavior Vol. 3 page： 920-922 2008

Induction of a small heat shock protein and its functional roles in Nicotiana plants in the defense response against Ralstonia solanacearum(1[W]) Reviewed

Milimo Maimbo, Kouhei Ohnishi, Yasufumi Hikichi, Hirofumi Yoshioka, Akinori Kiba

PLANT PHYSIOLOGY Vol. 145 ( 4 ) page： 1588 - 1599 2007.12

Language：English Publishing type：Research paper (scientific journal) Publisher：AMER SOC PLANT BIOLOGISTS

In tobacco (Nicotiana tabacum), Ralstonia solanacearum OE1-1 (RsOE1-1) is pathogenic, whereas R. solanacearum 8107 (Rs8107) is nonpathogenic and induces the hypersensitive response (HR). To elucidate the molecular mechanisms of plant-R. solanacearum interactions, we used differential display to isolate a cDNA fragment, A6, regulated in tobacco by inoculation with RsOE1-1. The deduced amino acid sequence predicted from full-length A6-cDNA showed similarity to small heat shock proteins from Arabidopsis (Arabidopsis thaliana; hypothetical protein), Medicago truncatula, and Cucumis melo; we therefore designated A6 to correspond to Ntshsp17 (for tobacco small heat shock protein 17). Recombinant Ntshsp17 overproduced in Escherichia coli exhibited molecular chaperone function. Expression of Ntshsp17 was increased in tobacco leaves inoculated with both RsOE1-1 and Rs8107. Expression was induced by heat treatment and by treatment with aminocyclopropane carboxylic acid, hydrogen peroxide, methyl jasmonate, and salicylic acid. Ntshsp17 expression was induced by inoculation with a HR and pathogenicity gene mutant of Rs8107 that does not induce the HR, but not by Agrobacterium-mediated transient expression of INF1, an HR elicitor. In Nbshsp17-silenced plants (an Ntshsp17 ortholog in Nicotiana benthamiana), expression of ETHYLENE-RESPONSE ELEMENT-BINDING PROTEIN, PATHOGENESIS-RELATED1a (PR1a), and PR4 genes was compromised, but expression of ELONGATION FACTOR1 alpha was scarcely affected. Appearance of the HR was not affected in the silenced plants. In the silenced plants, growth of Rs8107 was accelerated. Bacterial growth and wilt symptoms elicited by RsOE1-1 were also accelerated in the silenced plants. These results indicate that this small heat shock protein might have a role in HR-independent defenses in Nicotiana plants.

DOI： 10.1104/pp.107.105353

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PubMed

Regulation of rice NADPH oxidase by binding of Rac GTPase to its N-terminal extension Reviewed

Hann Ling Wong, Reinhard Pinontoan, Kokoro Hayashi, Ryo Tabata, Takashi Yaeno, Kana Hasegawa, Chojiro Kojima, Hirofumi Yoshioka, Koh Iba, Tsutomu Kawasaki, Ko Shimamoto

PLANT CELL Vol. 19 ( 12 ) page： 4022 - 4034 2007.12

Language：English Publishing type：Research paper (scientific journal) Publisher：AMER SOC PLANT BIOLOGISTS

Reactive oxygen species (ROS) produced by NADPH oxidase play critical roles in various cellular activities, including plant innate immunity response. In contrast with the large multiprotein NADPH oxidase complex of phagocytes, in plants, only the homologs of the catalytic subunit gp91(phox) and the cytosolic regulator small GTPase Rac are found. Plant homologs of the gp91phox subunit are known as Rboh (for respiratory burst oxidase homolog). Although numerous Rboh have been isolated in plants, the regulation of enzymatic activity remains unknown. All rboh genes identified to date possess a conserved N-terminal extension that contains two Ca2+ binding EF-hand motifs. Previously, we ascertained that a small GTPase Rac (Os Rac1) enhanced pathogen-associated molecular pattern-induced ROS production and resistance to pathogens in rice (Oryza sativa). In this study, using yeast two-hybrid assay, we found that interaction between Rac GTPases and the N-terminal extension is ubiquitous and that a substantial part of the N-terminal region of Rboh, including the two EF-hand motifs, is required for the interaction. The direct Rac-Rboh interaction was supported by further studies using in vitro pull-down assay, a nuclear magnetic resonance titration experiment, and in vivo fluorescence resonance energy transfer (FRET) microscopy. The FRET analysis also suggests that cytosolic Ca2+ concentration may regulate Rac-Rboh interaction in a dynamic manner. Furthermore, transient coexpression of Os Rac1 and rbohB enhanced ROS production in Nicotiana benthamiana, suggesting that direct Rac-Rboh interaction may activate NADPH oxidase activity in plants. Taken together, the results suggest that cytosolic Ca2+ concentration may modulate NADPH oxidase activity by regulating the interaction between Rac GTPase and Rboh.

DOI： 10.1105/tpc.107.055624

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PubMed

Involvement of mitogen-activated protein kinase in the induction of StrbohC and StrbohD genes in response to pathogen signals in potato Reviewed

Chihiro Yamamizo, Noriyuki Doke, Hirofumi Yoshioka, Kazuhito Kawakita

Journal of General Plant Pathology Vol. 73 ( 5 ) page： 304 - 313 2007.10

Language：English Publishing type：Research paper (scientific journal)

The respiratory burst oxidase homolog (rboh), a plant homolog of the catalytic subunit of phagocyte NADPH oxidase (gp91phox ), has been implicated in O2- generation. In the previous study, we reported that StrbohA is constitutively expressed at a low level, whereas StrbohB is upregulated after pathogen signals are perceived in potato tubers. To gain a better understanding about the role of rboh in the defense response in potato leaves, we investigated the regulatory mechanisms of the gene. StrbohB gene was activated in response to an avirulent race of Phytophthora infestans or to hyphal wall components (HWC) from the pathogen
however, levels of expression were very low. We isolated two novel rboh cDNAs, StrbohC and StrbohD, by virtue of the sequence information from the tomato whitefly-induced gene 1 (LeWfi1) and tomato TC121349, respectively. The expression of StrbohC and StrbohD was drastically upregulated in the potato leaves inoculated with P. infestans or treated with HWC elicitor. Mitogen-activated protein kinase (MAPK) is known to play a pivotal role in the oxidative burst. The transcript accumulation induced by HWC treatment was blocked by U0126, which is a specific inhibitor of MAPK kinase (MAPKK). Conversely, the StrbohC and StrbohD genes were upregulated, driven by a pathogen-inducible promoter, in transgenic potato leaves that carry a constitutively active mutant of the MAPKK, StMEK1 DD, that activates defense-related MAPKs. These results suggest that a MAPK cascade is involved in the process leading to the expression of the StrbohC and StrbohD genes. © 2007 The Phytopathological Society of Japan and Springer.

DOI： 10.1007/s10327-007-0020-1

Scopus

Calcium-dependent protein kinases regulate the production of reactive oxygen species by potato NADPH oxidase Reviewed

Michie Kobayashi, Ikuko Ohura, Kazuhito Kawakita, Naohiko Yokota, Masayuki Fujiwara, Ko Shimamoto, Noriyuki Doke, Hirofumi Yoshioka

PLANT CELL Vol. 19 ( 3 ) page： 1065 - 1080 2007.3

Language：English Publishing type：Research paper (scientific journal) Publisher：AMER SOC PLANT BIOLOGISTS

Reactive oxygen species (ROS) are implicated in plant innate immunity. NADPH oxidase (RBOH; for Respiratory Burst Oxidase Homolog) plays a central role in the oxidative burst, and EF-hand motifs in the N terminus of this protein suggest possible regulation by Ca2+. However, regulatory mechanisms are largely unknown. We identified Ser-82 and Ser-97 in the N terminus of potato (Solanum tuberosum) St RBOHB as potential phosphorylation sites. An anti-phosphopeptide antibody (pSer82) indicated that Ser-82 was phosphorylated by pathogen signals in planta. We cloned two potato calcium-dependent protein kinases, St CDPK4 and St CDPK5, and mass spectrometry analyses showed that these CDPKs phosphorylated only Ser-82 and Ser-97 in the N terminus of St RBOHB in a calcium-dependent manner. Ectopic expression of the constitutively active mutant of St CDPK5, St CDPK5VK, provoked ROS production in Nicotiana benthamiana leaves. The CDPK-mediated ROS production was disrupted by knockdown of Nb RBOHB in N. benthamiana. The loss of function was complemented by heterologous expression of wild-type potato St RBOHB but not by a mutant (S82A/S97A). Furthermore, the heterologous expression of St CDPK5VK phosphorylated Ser-82 of St RBOHB in N. benthamiana. These results suggest that St CDPK5 induces the phosphorylation of St RBOHB and regulates the oxidative burst.

DOI： 10.1105/tpc.106.048884

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PubMed

Non-destructive method to detect the cycle of lamination in sedimentary rocks: rhythmite sequence in Neoproterozoic Cap carbonates

Katsuta, N; Tojo, B; Takano, M; Yoshioka, H; Kawakami, S; Ohno, T; Kumazawa, M

RISE AND FALL OF THE EDIACARAN BIOTA Vol. 286 page： 27 - 34 2007

植物免疫におけるMAPキナーゼとラジカルバースト．植物における環境と生物ストレスに対する応答 Invited

吉岡博文, 浅井秀太

蛋白質核酸酵素増刊号 Vol. 52 page： 667-672 2007

植物病原細菌の感染を制御する宿主応答機構～ Ralstonia solanacearum- Nicotiana属植物相互作用の解析～ Invited

木場章範, Maimbo, M, 小森大輔, 張宛果, 富山裕美, 高橋彰子, 大西浩平, 吉岡博文, 曳地康史

植物－病原微生物の相互作用のダイナミズム Vol. 43 page： 55-65 2007

植物免疫におけるラジカルバーストの分子機構 Invited

吉岡博文, 浅井秀太, 間瀬圭介, 市川達士, 小林光智衣, 小八重善裕

植物－病原微生物の相互作用のダイナミズム Vol. 43 page： 37-47 2007

Nitrate reductase is responsible for elicitin-induced nitric oxide production in nicotiana benthamiana Reviewed

Ayako Yamamoto-Katou, Shinpei Katou, Hirofumi Yoshioka, Noriyuki Doke, Kazuhito Kawakita

PLANT AND CELL PHYSIOLOGY Vol. 47 ( 6 ) page： 726 - 735 2006.6

Language：English Publishing type：Research paper (scientific journal) Publisher：OXFORD UNIV PRESS

Recent works have established a key role for nitric oxide (NO) in activating disease resistance in plants. Nitrate reductase (NR) is one of the enzymes that are capable of producing NO in plants. In a previous study, we reported that pathogen signals induce expression of NR genes in potato, suggesting the involvement of NR in NO production induced by pathogen signals. In this study, we cloned NR genes from Nicotiana benthamiana and investigated their involvement in NO production induced by INF1, a major elicitin secreted by Phytophthora infestans. Treatment of protoplasts prepared from N. benthamiana leaves with INF1 elevated NO production to a maximum level 1-3 h after treatment. INF1-induced NO generation was suppressed completely by an NO-specific scavenger, but partially by a nitric oxide synthase inhibitor. To investigate the involvement of NR in INF1-induced NO production, NR genes were silenced by virus-induced gene silencing. The NR-silenced plants showed yellowish leaves which resemble the characteristic of Arabidopsis NR double mutants. Silencing of NR genes significantly decreased both NO2--producing activity and INF1-induced NO production, indicating that NR is involved in INF1-induced NO production. In contrast, overexpression of NbNR1 encoding N. benthamiana NR by Agrobacterium-mediated transient expression elevated NO2--producing activity nine times over the control; however, INF1-induced NO production in protoplasts overexpressing NbNR1 was comparable with that in control protoplasts. These results suggest that NR is involved in INF1-induced NO production, and post-translational modification of NR or availability of substrate NO2- may be a rate-limiting step of NO production by NR.

DOI： 10.1093/pcp/pcj044

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PubMed

Peroxynitrite generation and tyrosine nitration in defense responses in tobacco BY-2 cells

Syuhei Saito, Ayako Yamamoto-Katou, Hirofumi Yoshioka, Noriyuki Doke, Kazuhito Kawakita

PLANT AND CELL PHYSIOLOGY Vol. 47 ( 6 ) page： 689 - 697 2006.6

Language：English Publishing type：Research paper (scientific journal) Publisher：OXFORD UNIV PRESS

Peroxynitrite (ONOO-) is a compound formed by reaction of superoxide (O-2 (-)) with nitric oxide (NO) and is expected to possess characteristics of both O-2 (-) reactivity and NO mobility in order to function as a signal molecule. Although there are several reports that describe the role of ONOO- in defense responses in plants, it has been very difficult to detect ONOO- in bioimaging due to its short half-life or paucity of methods for ONOO--specific detection among reactive oxygen species or free radicals. Aminophenyl fluorescein (APF), a recently developed novel fluorophore for direct detection of ONOO- in bioimaging, was used for intracellular ONOO- detection. ONOO- generation in tobacco BY-2 cells treated with INF1, the major elicitin secreted by the late blight pathogen Phytophthora infestans, occurred within 1 h and reached a maximum level at 6-12 h after INF1 treatment. Urate, a ONOO- scavenger, abolished INF1-induced ONOO- generation. It is well known that ONOO- reacts with tyrosine residues in proteins to form nitrotyrosine in a nitration reaction as an ONOO--specific reaction. Western blot analysis using anti-nitrotyrosine antibodies recognized nitrotyrosine-containing proteins in 20 and 50 kDa bands in BY-2 protein extract containing SIN-1 [3-(4-morpholinyl) sydnonimine hydrochloride; an ONOO- donor]. These bands were also recognized in INF1-treated BY-2 cells and were found to be slightly suppressed by urate. Our study is the first to report ONOO- detection and tyrosine nitration in defense responses in plants.

DOI： 10.1093/pcp/pcj038

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PubMed

Loss of AtPDR8, a plasma membrane ABC transporter of Arabidopsis thaliana, causes hypersensitive cell death upon pathogen infection Reviewed

Y Kobae, T Sekino, H Yoshioka, T Nakagawa, E Martinoia, M Maeshima

PLANT AND CELL PHYSIOLOGY Vol. 47 ( 3 ) page： 309 - 318 2006.3

Language：English Publishing type：Research paper (scientific journal) Publisher：OXFORD UNIV PRESS

Plants contain a large number of ATP-binding cassette (ABC) transporters belonging to different subclasses. AtPDR8 is the only member of the pleiotropic drug resistance (PDR) ABC transporter subclass in Arabidopsis that is constitutively highly expressed. In transgenic Arabidopsis plants harboring the AtPDR8 promoter fused to beta-glucuronidase (GUS), reporter expression was shown to he strong in the stomata and hydathode. In the stomata, transcripts of AtPDR8 were particularly frequent in the cells surrounding air spaces. Subcellular fractionation and immunochemical analysis showed that AtPDR8 was localized in the plasma membrane. When a knockout mutant of AtPDR8 (atpdr8) was infected with bacterial and oomycete pathogens, the plants exhibited chlorotic lesions and a hypersensitive response (HR)-like cell death. Cell death was detected in the atpdr8 mutants within 10 h of infection with the virulent bacterial pathogen, Pseudomonas syringae. As a result, the growth of P syringae in the leaves of the atpdr8 mutant was reduced to 1% of that in the wild type. The defense response genes, PR-1, PR-2, PR-5, VPE gamma, AtrbohD and AtrbohF were highly expressed when the mutant plants were grown under non-sterile conditions. The expression of the AtPDR8 gene was enhanced by infection of virulent and avirulent bacterial pathogens. Our results indicate that AtPDR8 is a key factor controlling the extent of cell death in the defense response and suggest that AtPDR8 transports some substance(s) which is closely related to the response of plants to pathogens.

DOI： 10.1093/pcp/pcj001

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PubMed

Rewiring mitogen-activated protein kinase cascade by positive feedback confers potato blight resistance Reviewed

C Yamamizo, K Kuchimura, A Kobayashi, S Katou, K Kawakita, JDG Jones, N Doke, H Yoshioka

PLANT PHYSIOLOGY Vol. 140 ( 2 ) page： 681 - 692 2006.2

Language：English Publishing type：Research paper (scientific journal) Publisher：AMER SOC PLANT BIOLOGISTS

Late blight, caused by the notorious pathogen Phytophthora infestans, is a devastating disease of potato (Solanum tuberosum) and tomato (Solanum lycopersicum), and during the 1840s caused the Irish potato famine and over one million fatalities. Currently, grown potato cultivars lack adequate blight tolerance. Earlier cultivars bred for resistance used disease resistance genes that confer immunity only to some strains of the pathogen harboring corresponding avirulence gene. Specific resistance gene-mediated immunity and chemical controls are rapidly overcome in the field when new pathogen races arise through mutation, recombination, or migration from elsewhere. A mitogen-activated protein kinase (MAPK) cascade plays a pivotal role in plant innate immunity. Here we show that the transgenic potato plants that carry a constitutively active form of MAPK kinase driven by a pathogen-inducible promoter of potato showed high resistance to early blight pathogen Alternaria solani as well as P. infestans. The pathogen attack provoked defense-related MAPK activation followed by induction of NADPH oxidase gene expression, which is implicated in reactive oxygen species production, and resulted in hypersensitive response-like phenotype. We propose that enhancing disease resistance through altered regulation of plant defense mechanisms should be more durable and publicly acceptable than engineering overexpression of antimicrobial proteins.

DOI： 10.1104/pp.105.074906

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PubMed

Disruption of AtPDR8, a plasma membrane ABC transporter of Arabidopsis, exhibits hypersensitive cell death by pathogen infection. Reviewed

Kobae, Y, Yoshioka, H, Sekino, T, Nakanishi, Y, Martinoia, E, Maeshima, M

Plant & Cell Physiology Vol. 47 page： 309-318 2006

Subcellular localization of Strboh proteins and NADPH-dependent O 2--generating activity in potato tuber tissues Reviewed

Michie Kobayashi, Kazuhito Kawakita, Masayoshi Maeshima, Noriyuki Doke, Hirofumi Yoshioka

Journal of Experimental Botany Vol. 57 ( 6 ) page： 1373 - 1379 2006

Language：English Publishing type：Research paper (scientific journal)

Rapid generation of reactive oxygen species (ROS) at the cell surface has been implicated in plant defence responses. Genetic evidence indicates that a plant NADPH oxidase (Rboh
respiratory burst oxidase homologue) is associated with oxidative burst. However, there is not enough physiological evidence of Rboh localization available yet. Isozyme-specific antibodies against potato StrbohA and StrbohB (St
Solanum tuberosum) were prepared to investigate the localization of these proteins. Immunoblot analyses using potato microsomal proteins revealed that StrbohA was expressed constitutively at a low level, whereas the accumulation of StrbohB protein was induced by the cell wall elicitor of the potato pathogen Phytophthora infestans. It is demonstrated here that StrbohA and StrbohB are distributed in plasma membrane fractions which have been separated by sucrose density-gradient centrifugation using their specific antibodies. Green fluorescent protein-tagged Strboh proteins were also located on the plasma membrane by transient expression assay in onion epidermal cells. Additionally, NADPH-dependent O2--generating activities in plasma membrane fractions were diphenylene iodonium-sensitive and NaN 3-insensitive. These data suggest that StrbohA and StrbohB are predominantly localized on the plasma membrane and regulate ROS production in defence signalling. © The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved.

DOI： 10.1093/jxb/erj113

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Scopus

PubMed

植物の感染応答因子としてのNO． Invited

川北一人, 山本(加藤, 文子, 齋藤修平, 加藤新平, 加藤大明, 浅井秀太, 樹神博士, 吉岡博文, 道家紀志

病原体の寄生戦略と植物の応答 Vol. 42 page： 11-22 2006

Involvement of PPS3 phosphorylated by elicitor-responsive mitogen-activated protein kinases in the regulation of plant cell death Reviewed

S Katou, H Yoshioka, K Kawakita, O Rowland, JDG Jones, H Mori, N Doke

PLANT PHYSIOLOGY Vol. 139 ( 4 ) page： 1914 - 1926 2005.12

Language：English Publishing type：Research paper (scientific journal) Publisher：AMER SOC PLANT BIOLOGISTS

Mitogen-activated protein kinase (MAPK) cascades play pivotal roles in plant innate immunity. Overexpression of StMEK1(DD), a constitutively active MAPK kinase that activates salicylic acid-induced protein kinase (SIPK) and wound-induced protein kinase (WIPK), provokes hypersensitive response-like cell death in Nicotiana benthamiana. Here we purified a 51-kD MAPK, which was activated in potato (Solanum tuberosum) tubers treated with hyphal wall elicitor of a plant pathogen, and isolated the cDNA designated StMPK1. The deduced amino acid sequence of the StMPK1 showed strong similarity to stress-responsive MAPKs, such as tobacco (Nicotiana tabacum) SIPK and Arabidopsis (Arabidopsis thaliana) AtMPK6. To investigate the downstream signaling of StMPK1, we identified several proteins phosphorylated by StMPK1 (PPSs) using an in vitro expression cloning method. To dissect the biological function of PPSs in the plant defense, we employed virus-induced gene silencing (VIGS) in N. benthamiana. VIGS of NbPPS3 significantly delayed cell death induced by the transient expression of StMEK1(DD) and treatment with hyphal wall elicitor. Furthermore, the mobility shift of NbPPS3 on SDS-polyacrylamide gel was induced by transient expression of StMEK1(DD). The mobility shift of NbPPS3 induced by StMEK1(DD) was not compromised by VIGS of WIPK or SIPK alone, but drastically reduced by the silencing of both WIPK and SIPK. This work strongly supports the idea that PPS3 is a physiological substrate of StMPK1 and is involved in cell death activated by a MAPK cascade.

DOI： 10.1104/pp.105.066795

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PubMed

抵抗性遺伝子を介した防御応答におけるシグナル伝達機構． Invited

吉岡博文, 山溝千尋, 小林光智衣, 水野貴仁, 川北一人, 道家紀志

植物・病原体相互反応における特異性決定機構 Vol. 41 page： 13-22 2005.8

The Arabidopsis gene CAD1 controls programmed cell death in the plant immune system and encodes a protein containing a MACPF domain Reviewed

C Morita-Yamamuro, T Tsutsui, M Sato, H Yoshioka, M Tamaoki, D Ogawa, H Matsuura, T Yoshihara, A Ikeda, Uyeda, I, J Yamaguchi

PLANT AND CELL PHYSIOLOGY Vol. 46 ( 6 ) page： 902 - 912 2005.6

Language：English Publishing type：Research paper (scientific journal) Publisher：OXFORD UNIV PRESS

To clarify the processes involved in plant immunity, we have isolated and characterized a single recessive Arabidopsis mutant, cad1 (constitutively activated cell death 1), which shows a phenotype that mimics the lesions seen in the hypersensitive response (HR). This mutant shows spontaneously activated expression of pathogenesis-related (PR) genes, and leading to a 32-fold increase in salicylic acid (SA). Inoculation of cad1 mutant plants with Pseudomonas syringae pv tomato DC3000 shows that the cad1 mutation results in the restriction of bacterial growth. Cloning of CAD1 reveals that this gene encodes a protein containing a domain with significant homology to the MACPF (membrane attack complex and perforin) domain of complement components and perforin proteins that are involved in innate immunity in animals. Furthermore, cell death is suppressed in transgenic cad1 plants expressing nahG, which encodes an SA-degrading enzyme. We therefore conclude that the CAD1 protein negatively controls the SA-mediated pathway of programmed cell death in plant immunity.

DOI： 10.1093/pcp/pci095

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PubMed

Primary lung adenocarcinoma with heterotopic bone formation.

Usami N, Yoshioka H, Mori S, Imaizumi M, Nagasaka T, Ueda Y

The Japanese journal of thoracic and cardiovascular surgery : official publication of the Japanese Association for Thoracic Surgery = Nihon Kyobu Geka Gakkai zasshi Vol. 53 ( 2 ) page： 102 - 5 2005.2

Functional analysis of Avr9/Cf-9 rapidly elicited genes identifies a protein kinase, ACIK1, that is essential for full Cf-9-dependent disease resistance in tomato Reviewed

O Rowland, AA Ludwig, CJ Merrick, F Baillieul, FE Tracy, WE Durrant, L Fritz-Laylin, Nekrasov, V, K Sjolander, H Yoshioka, JDG Jones

PLANT CELL Vol. 17 ( 1 ) page： 295 - 310 2005.1

Language：English Publishing type：Research paper (scientific journal) Publisher：AMER SOC PLANT BIOLOGISTS

Tomato (Lycopersicon esculentum) Cf genes confer resistance to the fungal pathogen Cladospodium fulvum through recognition of secreted avirulence (Avr) peptides. Plant defense responses, including rapid alterations in gene expression, are immediately activated upon perception of the pathogen. Previously, we identified a collection of Avr9/Cf-9 rapidly (15 to 30 min) elicited (ACRE) genes from tobacco (Nicotiana tabacum). Many of the ACRE genes encode putative signaling components and thus may play pivotal roles in the initial development of the defense response. To assess the requirement of 42 of these genes in the hypersensitive response (HR) induced by Cf-9/Avr9 or by Cf-4/Avr4, we used virus-induced gene silencing (VIGS) in N. benthamiana. Three genes were identified that when silenced compromised the Cf-mediated HR. We further characterized one of these genes, which encodes a Ser/Thr protein kinase called Avr9/Cf-9 induced kinase 1 (ACIK1). ACIK1 mRNA was rapidly upregulated in tobacco and tomato upon elicitation by Avr9 and by wounding. Silencing of ACIK1 in tobacco resulted in a reduced HR that correlated with loss of ACIK1 transcript. Importantly, ACIK1 was found to be required for Cf-9/Avr9- and Cf-4/Avr4-mediated HRs but not for the HR or resistance mediated by other resistance/Avr systems, such as Pto/AvrPto, Rx/Potato virus X, or N/Tobacco mosaic virus. Moreover, VIGS of LeACIK1 in tomato decreased Cf-9-mediated resistance to C. fulvum, showing the importance of ACIK1 in disease resistance.

DOI： 10.1105/tpc.104.026013

Web of Science

Role of calcium ion in plant innate immunity

Yoshioka, H; Ooura, I; Kawakita, K; Doke, N

PLANT AND CELL PHYSIOLOGY Vol. 46 page： S23 - S23 2005

植物における活性酸素の役割とその誘導．活性酸素，－生産と防御のメカニズム－． Invited

吉岡博文, 山溝千尋

蛋白質核酸酵素 Vol. 50 page： 310-315 2005

Involvement of nitric oxide generation in hypersensitive cell death induced by elicitin in tobacco cell suspension culture.

Yamamoto, A, Katou, S, Yoshioka, H, Doke, N, Kawakita, K

Journal of Genaral Plant Pathology Vol. 70 page： 85-92 2004.1

Cytosolic HSP90 and HSP70 are essential components of INF1-mediated hypersensitive response and non-host resistance to Pseudomonas cichorii in Nicotiana benthamiana

Kanzaki, H; Saitoh, H; Ito, A; Fujisawa, S; Kamoun, S; Katou, S; Yoshioka, H; Terauchi, R

MOLECULAR PLANT PATHOLOGY Vol. 4 ( 5 ) page： 383 - 391 2003.9

Disease stress-inducible genes of tobacco: expression profile of elicitor-responsive genes isolated by subtractive hybridization

Takemoto, D; Yoshioka, H; Doke, N; Kawakita, K

PHYSIOLOGIA PLANTARUM Vol. 118 ( 4 ) page： 545 - 553 2003.8

Commensurate-incommensurate transition in a one-dimensional dimerized electron system close to quarter-filling

Yoshioka, H; Tsuchiizu, M; Suzumura, Y

SYNTHETIC METALS Vol. 133 page： 49 - 50 2003.3

Elicitation of primary and secondary metabolism during defense in the potato. Reviewed

Nakane, E, Kawakita, K, Doke, N, Yoshioka, H

Journal of Genaral Plant Pathology Vol. 69 page： 378-384 2003.1

Nitrate reductase, a nitric oxide-producing enzyme: induction by pathogen signals. Reviewed

Yamamoto, A, Katou, S, Yoshioka, H, Doke, N, Kawakita, K

Journal of Genaral Plant Pathology Vol. 69 page： 218-229 2003.1

Salmonella pericarditis in a patient with primary idiopathic chylopericardium.

Yoshioka H, Shigemitsu K, Takeuchi M, Mori S, Imaizumi M, Ueda Y

The Japanese journal of thoracic and cardiovascular surgery : official publication of the Japanese Association for Thoracic Surgery = Nihon Kyobu Geka Gakkai zasshi Vol. 51 ( 1 ) page： 16 - 7 2003.1

Direct interaction of OsRac with NADPH oxidase regulates ROS production in defense signaling of rice

Pinontoan, R; Yoshioka, H; Kawasaki, T; Shimamoto, K

PLANT AND CELL PHYSIOLOGY Vol. 44 page： S178 - S178 2003

Functional analysis of potato mitogen-activated protein kinase kinase, StMEK1. Reviewed

Katou, S, Yamamoto, A, Yoshioka. H, Kawakita, K, Doke, N

Journal of General Plant Pathology Vol. 69 ( 3 ) page： 161-168 - 168 2003

宿主植物の抵抗性制御と糸状菌病に対する耐病性強化の展望． Invited

吉岡博文, 口村和男, 山溝千尋, 池田直希, 加藤新平, 川北一人, 小林晃, 道家紀志

作物の耐病性強化戦略と植物－病原体相互作用の分子機構研究 Vol. 39 page： 101-110 2003

植物免疫としてのオキシダティブバーストを探る，－MAPKカスケードが活性酸素の生成と細胞死を制御する－． Invited

吉岡博文

化学と生物 Vol. 41 page： 639-641 2003

Perception of pathogen signals and molecular mechanisms for the oxidative burst

Yoshioka, H

PLANT AND CELL PHYSIOLOGY Vol. 43 page： S10 - S10 2002

Induction of plant gp91 phox homolog by fungal cell wall, arachidonic acid, and salicylic acid in potato. Reviewed

Yoshioka, H, Sugie, K, Park, H.-J, Maeda, H, Tsuda, N, Kawakita, K, Doke, N

Molecular Plant-Microbe Interactions Vol. 14 page： 725-736 2001.1

The oxidative burst in plants: Mechanism and function in induced resistance

Doke, N; Park, HJ; Katou, S; Komatsubara, H; Makino, T; Ban, S; Sugie, K; Yoshioka, H; Kawakita, K

DELIVERY AND PERCEPTION OF PATHOGEN SIGNALS IN PLANTS page： 184 - 193 2001

Transport properties of disordered carbon nanotubes with long-range Coulomb interaction

Yoshioka, H

PHYSICAL REVIEW B Vol. 61 ( 11 ) page： 7316 - 7319 2000.3

CDNA cloning of sesquiterpene cyclase and squalene synthase, and expression of the genes in potato tuber infected with Phytophthora infestans Reviewed

H Yoshioka, N Yamada, N Doke

PLANT AND CELL PHYSIOLOGY Vol. 40 ( 9 ) page： 993 - 998 1999.9

Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOC PLANT PHYSIOLOGISTS

Sesquiterpene cyclase and squalene synthase are key branch point enzymes in isoprenoid pathway for the synthesis of sesquiterpenoid phytoalexins and sterols/steroid glycoalkaloids, respectively. cDNA clones encoding these enzymes were isolated from potato, A phylogenetic tree showed that the sesquiterpene cyclase is vetispiradiene synthase. Infection of Phytophthora infestans with potato tubers caused transient increases in the transcript level of vetispiradiene synthase in a compatible and an incompatible interactions. On the other hand, wound-induced expression of the squalene synthase was suppressed in favor of the expression of vetispiradiene synthase regardless of inoculated races.

DOI： 10.1093/oxfordjournals.pcp.a029633

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PubMed

A 51 kDa protein kinase of potato activated with hyphal wall components from Phytophthora infestans

Katou, S; Senda, K; Yoshioka, H; Doke, N; Kawakita, K

PLANT AND CELL PHYSIOLOGY Vol. 40 ( 8 ) page： 825 - 831 1999.8

Resistance of carbon nanotubes connected to reservoirs

Yoshioka, H; Odintsov, AA

SYNTHETIC METALS Vol. 103 ( 1-3 ) page： 2527 - 2528 1999.6

Theoretical study on the charge gap of organic conductor - Bechgaard salts

Tsuchiizu, M; Yoshioka, H; Suzumura, Y

JOURNAL OF THE PHYSICAL SOCIETY OF JAPAN Vol. 68 ( 6 ) page： 1809 - 1812 1999.6

Aspartate aminotransferase in alfalfa nodules: Localization of mRNA during effective and ineffective nodule development and promoter analysis

Yoshioka, H; Gregerson, RG; Samac, DA; Hoevens, KCM; Trepp, G; Gantt, JS; Vance, CP

MOLECULAR PLANT-MICROBE INTERACTIONS Vol. 12 ( 4 ) page： 263 - 274 1999.4

Persistent current and correlation effects in carbon nanotubes

Odintsov, AA; Smit, W; Yoshioka, H

EUROPHYSICS LETTERS Vol. 45 ( 5 ) page： 598 - 604 1999.3

NADH-glutamate synthase in alfalfa root nodules. Genetic regulation and cellular expression

Trepp, GB; van de Mortel, M; Yoshioka, H; Miller, SS; Samac, DA; Gantt, JS; Vance, CP

PLANT PHYSIOLOGY Vol. 119 ( 3 ) page： 817 - 828 1999.3

Effect of cytochalasin D on defense responses of potato tuber discs treated with hyphal wall components of Phytophthora infestans

Takemoto, D; Maeda, H; Yoshioka, H; Doke, N; Kawakita, K

PLANT SCIENCE Vol. 141 ( 2 ) page： 219 - 226 1999.2

Aspartate aminotransferase in alfalfa nodules: Localization of mRNA during effective and ineffective nodules development and promoter analysis. Reviewed

Yoshioka, H, Gregerson, R.G, Samac, D.A, Hoevens, K.C.M, Trepp, G, Gantt, J.S, Vance, C.P

Molecular Plant-Microbe Interactions Vol. 12 page： 263-274 1999.1

NADH-glutamate synthase in alfalfa root nodules: genetic regulation and cellular expression. Reviewed

Trepp, G, van de Mortel, M, Yoshioka, H, Miller, S.S, Samac, D.A, Gantt, J.S, Vance, C.P

Plant Physiology Vol. 119 page： 817-828 1999.1

Plasma membrane perturbation in association with calcium ion movement followed by fungal elicitor-stimulated oxidative burst and defense gene activation in potato tuber. Reviewed

Miura, Y, Yoshioka, H, Park, H.-J, Kawakita, K, Doke, N

Annals of the Phytopathological Society of Japan Vol. 65 ( 4 ) page： 447-453 - 453 1999.1

Language：English Publishing type：Research paper (scientific journal) Publisher：The Phytopathological Society of Japan (PSJ)

Chlortetracycline (CTC) is known to fluoresce when interacting with Ca^<2+> in hydrophobic environments such as membranes. CTC fluorescence in potato tubers was rapidly reduced by hyphal wall component-elicitor (HWC-elicitor) from Phytophthora infestans in a dose-dependent manner, suggesting that Ca^<2+> in the plasma membrane was released by the elicitor. Pre-treatments of the tuber slices with verapamil and EGTA, a Ca^<2+> channel blocker and an extracellular Ca^<2+> chelator, respectively, suppressed the reduction of CTC fluorescence induced by the HWC-elicitor. The reduced fluorescence was preceded by an oxidative burst observed in the tuber tissues loaded with luminol after stimulation with HWC-elicitor. Application with HWC-elicitor also immediately reduced fluorescence of the CTC-loaded, Ca^<2+>-bound plasma membrane fraction in vitro. Moreover, RNA get blot analysis showed that verapamil and EGTA dramatically suppressed the accumulation of transcript for phenylalanine ammonia-lyase induced by the elicitor. These results demonstrate that extracellular Ca^<2+> is rapidly released from the plasma membrane by the treatment with HWC-elicitor and plays a key role in signal transduction leading to the oxidative burst and the activation of a defense gene.

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Other Link： http://agriknowledge.affrc.go.jp/RN/2010592250

Response functions of two-coupled chains of Tomonaga-Luttinger liquids

Yoshioka, H; Suzumura, Y

PHYSICA C Vol. 309 ( 1-2 ) page： 151 - 160 1998.12

ジャガイモ塊茎における防御遺伝子発現とそのシグナル伝達． Invited

吉岡博文, 伊藤夢子, 杉江顕一, 三浦由雄, 川北一人, 道家紀志

植物と病原体の情報応答機構 page： 49-108 1998

Analyses of phosphoenolpyruvate carboxylase gene structure and expression in alfalfa nodules

Pathirana, MS; Samac, DA; Roeven, R; Yoshioka, H; Vance, CP; Gantt, JS

PLANT JOURNAL Vol. 12 ( 2 ) page： 293 - 304 1997.8

Nitrogen assimilation in alfalfa: Isolation and characterization of an asparagine synthetase gene showing enhanced expression in root nodules and dark-adapted leaves

Shi, LF; Twary, SN; Yoshioka, H; Gregerson, RG; Miller, SS; Samac, DA; Gantt, JS; Unkefer, PJ; Vance, CP

PLANT CELL Vol. 9 ( 8 ) page： 1339 - 1356 1997.8

Properties of fluctuations in two coupled chains of Luttinger liquids

Yoshioka, H; Suzumura, Y

JOURNAL OF LOW TEMPERATURE PHYSICS Vol. 106 ( 1-2 ) page： 49 - 72 1997.1

Topological dislocations and mixed state of charge density waves

Hayashi, M; Yoshioka, H

PHYSICAL REVIEW LETTERS Vol. 77 ( 16 ) page： 3403 - 3406 1996.10

A cytosolic phospholipase A2 from potato tissues appears to be patatin. Reviewed

Senda, K, Yoshioka, H, Doke, N, Kawakita, K

Plant and Cell Physiology Vol. 37 page： 347-353 1996.1

ROLES OF INTERCHAIN HOPPING IN 2 COUPLED CHAINS OF TOMONAGA MODEL

YOSHIOKA, H; SUZUMURA, Y

JOURNAL OF THE PHYSICAL SOCIETY OF JAPAN Vol. 64 ( 10 ) page： 3811 - 3825 1995.10

Suppression of the activation of chitinase and β-1,3-glucanase in pea epicotyls by eogenous suppressor from Pisam sativum. Reviewed

Nasu, K, Yoshioka, H, Ichinose, Y, Yamada, T, Oku, H, Shiraishi, T

Annals of the Phytopathological Society of Japan Vol. 61 page： 13-17 1995.1

Suppression of phenylalanine ammonia-lyase mRNA accumulation by suppressors from Phytophthora infestans. Reviewed

Yoshioka, H, Hayakawa, Y, Doke, N

Annals of the Phytopathological Society of Japan Vol. 61 ( 1 ) page： 7-12 1995

THE X-RAY TELESCOPE ON BOARD ASCA

SERLEMITSOS, PJ; JALOTA, L; SOONG, Y; KUNIEDA, H; TAWARA, Y; TSUSAKA, Y; SUZUKI, H; SAKIMA, Y; YAMAZAKI, T; YOSHIOKA, H; FURUZAWA, A; YAMASHITA, K; AWAKI, H; ITOH, M; OGASAKA, Y; HONDA, H; UCHIBORI, Y

PUBLICATIONS OF THE ASTRONOMICAL SOCIETY OF JAPAN Vol. 47 ( 1 ) page： 105 - 114 1995

SUPPRESSORS - DETERMINANTS OF SPECIFICITY PRODUCED BY PLANT-PATHOGENS Invited Reviewed

T SHIRAISHI, T YAMADA, K SAITOH, T KATO, K TOYODA, H YOSHIOKA, HM KIM, Y ICHINOSE, M TAHARA, H OKU

PLANT AND CELL PHYSIOLOGY Vol. 35 ( 8 ) page： 1107 - 1119 1994.12

Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOC PLANT PHYSIOLOGISTS

Plant pathogens secrete suppressors that delay or prevent the host defense responses, with resultant conditioning of host cells such that they become susceptible even to avirulent or nonpathogenic microorganisms. Suppressors have been characterized as glycoproteins, glycopeptides, peptides and anionic and nonanionic glucans. A suppressor itself is non-toxic to plant cells and, thus, it can be distinguished from host-specific toxins produced by certain pathogens. Suppressors disturb fundamental functions of host plasma membranes. For example, the suppressor from a pea pathogen, Mycosphaerella pinodes, inhibits both the ATPase activity and polyphosphoinositide metabolism in pea plasma membranes, causing the temporary suppression of the signal-transduction pathway that leads to the expression of defense genes, which encode key enzymes in the biosynthetic pathway to phytoalexin. In this review, evidence for the role of suppressors in the determination of plant host-parasite specificity is summarized.

Web of Science

Race-cultivar specificity in plant fungal disease and its molecular mechanisms. Invited

Plant Cell Technology Vol. 5 ( 1 ) page： 19-27 1993

AN ENDOGENOUS SUPPRESSOR OF THE DEFENSE RESPONSE IN PISUM-SATIVUM Reviewed

K NASU, T SHIRAISHI, H YOSHIOKA, N HORI, Y ICHINOSE, T YAMADA, H OKU

PLANT AND CELL PHYSIOLOGY Vol. 33 ( 5 ) page： 617 - 626 1992.7

Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOC PLANT PHYSIOLOGISTS

Healthy pea plants contain a substance, tentatively called "endogenous suppressor", which specifically suppresses the accumulation of pisatin in pea plants that is induced by treatment with CuCl2 or an elicitor from Mycosphaerella pinodes. This suppressor elicits the accumulation of phytoalexins in other legumes, such as kidney bean, soybean and cowpea. The endogenous suppressor functions to delay the accumulation of pisatin, the activation of phenylalanine ammonialyase (PAL) and the accumulation of mRNAs for PAL and chalcone synthase induced by the elicitor from M. pinodes. The substance specifically induces susceptibility to nonpathogens, such as Mycosphaerella ligulicola and M. melonis, in pea out of four species of legume tested, but the effect is not cultivar-specific. Thus, the endogenous suppressor in healthy pea plants suppresses a series of self-defense reactions and induces susceptibility in pea plants in a species-specific manner, being similar to the exogenous fungal suppressor from the pea pathogen, M. pinodes.

Web of Science

PREPARATION OF THE INFECTION COURT BY ERYSIPHE-GRAMINIS F-SP HORDEI - CUTINASE IS A COMPONENT OF THE CONIDIAL EXUDATE Reviewed

SF PASCHOLATI, H YOSHIOKA, H KUNOH, RL NICHOLSON

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY Vol. 41 ( 1 ) page： 53 - 59 1992.7

REGULATION OF POLYPHOSPHOINOSITIDE METABOLISM IN PEA PLASMA-MEMBRANES BY ELICITOR AND SUPPRESSOR FROM A PEA PATHOGEN, MYCOSPHAERELLA-PINODES Reviewed

K TOYODA, T SHIRAISHI, H YOSHIOKA, T YAMADA, Y ICHINOSE, H OKU

PLANT AND CELL PHYSIOLOGY Vol. 33 ( 4 ) page： 445 - 452 1992.6

Language：English Publishing type：Research paper (scientific journal) Publisher：OXFORD UNIV PRESS

Effects of the elicitor and the suppressor from a pea pathogen, Mycosphaerella pinodes, on polyphosphoinositide metabolism in pea plasma membranes were examined in vitro. Lipid phosphorylation in the isolated pea plasma membrane was drastically stimulated by the elicitor, but markedly inhibited by the suppressor. A similar inhibitory effect was observed by the treatment with orthovanadate or K-252a that blocked pisatin production induced by the elicitor. Neomycin, an aminoglycoside antibiotic that interacts with the polyphosphoinositide metabolism, also affected the lipid phosphorylation in vitro and blocked the elicitor-induced accumulation of pisatin in vivo. These results suggest that rapid changes of polyphosphoinositide metabolism in pea plasma membranes is one of indispensable processes during the elicitation of defense responses.

Web of Science

ORTHOVANADATE SUPPRESSES ACCUMULATION OF PHENYLALANINE AMMONIA-LYASE MESSENGER-RNA AND CHALCONE SYNTHASE MESSENGER-RNA IN PEA EPICOTYLS INDUCED BY ELICITOR FROM MYCOSPHAERELLA-PINODES Reviewed

H YOSHIOKA, T SHIRAISHI, S KAWAMATA, K NASU, T YAMADA, Y ICHINOSE, H OKU

PLANT AND CELL PHYSIOLOGY Vol. 33 ( 2 ) page： 201 - 204 1992.3

Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOC PLANT PHYSIOLOGISTS

Orthovanadate delayed accumulation of mRNAs encoding phenylalanine ammonia-lyase and chalcone synthase in pea epicotyls induced by an elicitor from Mycosphaerella pinodes. However, accumulation of mRNA for a putative P-type ATPase was not affected. The relationship between the ATPase and defense responses is discussed.

Web of Science

Suppression of the activation of chitinase and β-1,3-glucanase in pea epicotyls by orthonadate and a suppressor from Mycosphaerella pinodes. Reviewed

Yoshioka, H, Shiraishi, T, Yamada, T, Nasu, K, Ichinose, Y, Oku, H

Annals of the Phytopathological Society of Japan Vol. 58 ( 3 ) page： 405-410 1992

SUPPRESSOR PRODUCTION AS A KEY FACTOR FOR FUNGAL PATHOGENESIS Reviewed

T SHIRAISHI, T YAMADA, H OKU, H YOSHIOKA

MOLECULAR STRATEGIES OF PATHOGENS AND HOST PLANTS page： 151 - 162 1991

エンドウ動的遺伝子の発現機構． Invited

山田哲治, 白石友紀, 一瀬勇規, 奥八郎, 川又伸治, 田中良和, Spirasertsak, P, 安成才, 吉岡博文, 橋本忠明

生物相互間の認識機構 Vol. 27 page： 56-65 1991

SUPPRESSION OF PISATIN PRODUCTION AND ATPASE ACTIVITY IN PEA PLASMA-MEMBRANES BY ORTHOVANADATE, VERAPAMIL AND A SUPPRESSOR FROM MYCOSPHAERELLA-PINODES Reviewed

H YOSHIOKA, T SHIRAISHI, T YAMADA, Y ICHINOSE, H OKU

PLANT AND CELL PHYSIOLOGY Vol. 31 ( 8 ) page： 1139 - 1146 1990.12

Language：English Publishing type：Research paper (scientific journal) Publisher：JAPANESE SOC PLANT PHYSIOLOGISTS

A pea pathogen, Mycosphaerella pinodes, secretes both an elicitor and a suppressor for the accumulation of pisatin, a major phytoalexin of pea, into the spore germination fluid. The effects of elicitor and the suppressor on the ATPase activity in pea plasma membranes was examined. The ATPase was sensitive to orthovanadate and dicyclohexylcarbodiimide but insensitive to nitrate and azide; it was unaffected by the elicitor but was markedly inhibited by the suppressor (50-mu-g.ml-1, bovine serum albumin equivalents) or verapamil (100-mu-M). The accumulation of pisatin induced by the elicitor was delayed for 3 to 6 h in the presence of orthovanadate or verapamil to an extent similar to that in the presence of the suppressor. The relationship between the inhibition of plasma membrane ATPase activity and the suppression of the active defense reaction that involves the production of pisatin in the pea plant is discussed.

Web of Science

PREPARATION OF THE INFECTION COURT BY ERYSIPHE-GRAMINIS - DEGRADATION OF THE HOST CUTICLE Reviewed

H KUNOH, RL NICHOLSON, H YOSIOKA, N YAMAOKA, KOBAYASHI, I

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY Vol. 36 ( 5 ) page： 397 - 407 1990.5

PREPARATION OF THE INFECTION COURT BY ERYSIPHE-GRAMINIS .1. CONTACT-MEDIATED CHANGES IN MORPHOLOGY OF THE CONIDIUM SURFACE Reviewed

H KUNOH, N YAMAOKA, H YOSHIOKA, RL NICHOLSON

EXPERIMENTAL MYCOLOGY Vol. 12 ( 4 ) page： 325 - 335 1988.12

PREPARATION OF THE INFECTION COURT BY ERYSIPHE-GRAMINIS .2. RELEASE OF ESTERASE ENZYME FROM CONIDIA IN RESPONSE TO A CONTACT STIMULUS Reviewed

RL NICHOLSON, H YOSHIOKA, N YAMAOKA, H KUNOH

EXPERIMENTAL MYCOLOGY Vol. 12 ( 4 ) page： 336 - 349 1988.12

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