Faculty Profiles - ASANUMA, Hiroyuki

Papers - ASANUMA, Hiroyuki

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In Vivo Efficacy and Safety of Systemically Administered Serinol Nucleic Acid-Modified Antisense Oligonucleotides in Mouse Kidney Reviewed

Tsuboi, T.; Hattori, K.; Ishimoto, T.; Imai, K.; Doke, T.; Hagita, J.; Ariyoshi, J.; Furuhashi, K.; Kato, N.; Ito, Y.; Kamiya, Y.; Asanuma, H.; Maruyama, S.

Mol. Ther. Nucleic Acids Vol. 36 page： 102387 2025.3

"Kinetics of Strand Displacement Reaction with Acyclic Artificial Nucleic Acids Reviewed

Makino, K.; Sugiyama, I.; Asanuma, H.; Kashida, H.

Angew. Chem. Int. Ed. Engl. Vol. 63 page： e202319864 2024.7

A Light-powered single-stranded DNA molecular motor with colour-selective single-step control Reviewed International coauthorship

Anderson, T.; Wu, W.; Sirbu, O.; Tong, K.; Winna, S.; Liu, X. R.; Kou, B.; Murayama, K.; Asanuma, H.; Wang, Z.

Angew. Chem. Int. Ed. Engl. Vol. 63 page： e202405250 2024.6

Site-selective photo-crosslinking of stilbene pairs in a DNA duplex mediated by ruthenium photocatalyst Invited Reviewed

Kashida, H.; Azuma, H.; Sotome, H.; Miyasaka, H.; Asanuma, H.

Angewandte Chemie International Edition Vol. 63 page： e202319516 2024.1

Unexpectedly stable homopurine parallel triplex of SNA:RNA*SNA and L-aTNA:RNA*L-aTNA Reviewed

Kamiya, Y.; Lao, S.; Ariyoshi, J.; Sato, F.; Asanuma, H.

Chemical Communications Vol. 60 page： 1257 - 1260 2023.12

Rapid Chemical Ligation of DNA and Acyclic Threoninol Nucleic Acid (aTNA) for Effective Nonenzymatic Primer Extension Reviewed

Okita, H.; Kondo, S.; Murayama, K.; Asanuma, H.

Journal of the American Chemical Society Vol. 145 page： 17872 - 17880 2023.7

Syntheses of base-labile pseudo-complementary SNA and L-aTNA phosphoramidite monomers Invited Reviewed

Sato, F.; Kamiya, Y.; Asanuma, H.

J. Org. Chem. Vol. 88 page： 796 - 804 2023.1

Cooperative cargo transportation by a swarm of molecular machines Reviewed

Akter, M.; Keya, J. J.; Kayano, K.; Kabir, A. M. R.; Inoue, D.; Hess, H.; Sada, K.; Kuzuya, A.; Asanuma, H.; Kakugo, A.

Sci. Robot. Vol. 7 page： eabm0677 2022.5

Xeno nucleic acids (XNAs) having non-ribose scaffolds with unique supramolecular properties Invited Reviewed

Asanuma, H.; Kamiya, Y.; Kashida, H.; Murayama, K.

Vol. 28 page： 3933 2022.3

Color-Changing Fluorescent Barcode Based on Strand Displacement Reaction Enables Simple Multiplexed Labeling Reviewed

Makino, K.; Susaki, E.; Endo, M.; Asanuma, H.; Kashida, H.

Journal of the American Chemical Society Vol. 144 page： 1572 - 1579 2022.1

Orthogonal Amplification Circuits Composed of Acyclic Nucleic Acids Enable RNA Detection Reviewed

Chen Y., Nagao R., Murayama K., Asanuma H.

Journal of the American Chemical Society Vol. 144 page： 5887 - 5892 2022

Authorship：Last author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of the American Chemical Society

Construction of complex DNA circuits is difficult due to unintended hybridization and degradation by enzymes under biological conditions. We herein report a hybridization chain reaction (HCR) circuit composed of left-handed acyclic d-threoninol nucleic acid (d-aTNA), which is orthogonal to right-handed DNA and RNA. Because of its high thermal stability, use of an aTNA hairpin with a short 7 base-pair stem ensured clear ON-OFF control of the HCR circuit. The aTNA circuit was stable against nucleases. A circuit based on right-handed acyclic l-threoninol nucleic acid (l-aTNA) was also designed, and high orthogonality between d- and l-aTNA HCRs was confirmed by activation of each aTNA HCR via a corresponding input strand. A dual OR logic gate was successfully established using serinol nucleic acid (SNA), which could initiate both d- and l-aTNA circuits. The d-aTNA HCR was used for an RNA-dependent signal amplification system via the SNA interface. The design resulted in 80% yield of the cascade reaction in 3000 s without a significant leak. This work represents the first example of use of heterochiral HCR circuits for detection of RNA molecules. The method has potential for direct visualization of RNA in vivo and the FISH method.

DOI： 10.1021/jacs.1c12659

Scopus

Perylene-Cy3 FRET System to Analyze Photoactive DNA Structures Reviewed

Kawai H., Doi T., Ito Y., Kameyama T., Torimoto T., Kashida H., Asanuma H.

Chemistry - A European Journal Vol. 27 ( 50 ) page： 12845 - 12850 2021.9

Authorship：Last author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Chemistry - A European Journal

We report a new Förster resonance energy transfer (FRET) system for structural analyses of DNA duplexes using perylene and Cy3 as donor and acceptor, respectively, linked at the termini of a DNA duplex via D-threoninol. Experimentally obtained FRET efficiencies were in good agreement with theoretical values calculated based on canonical B-form DNA. Due to the relatively long Förster radius, this system can be used to analyze large DNA structures, and duplexes containing photo-reactive molecules can be analyzed since perylene can be excited with visible light. The system was used to analyze a DNA duplex containing stilbene, demonstrating that in the region of the stilbene cluster the duplex adopts a ladder-like structure rather than helical one. Upon photodimerization between stilbene residues, FRET efficiencies indicated the reaction does not disturb DNA duplex. This FRET system will be useful for analysis of photoreactions of nucleobases as well as a wide range of nucleic acid structures.

DOI： 10.1002/chem.202101738

Scopus

PubMed

Design and Hybridization Properties of Acyclic Xeno Nucleic Acid Oligomers Invited Reviewed

Murayama K., Asanuma H.

ChemBioChem Vol. 22 ( 15 ) page： 2507 - 2515 2021.8

Authorship：Last author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：ChemBioChem

Xeno nucleic acids (XNAs) are analogues of DNA and RNA that have a non-ribose artificial scaffold. XNAs are possible prebiotic genetic carriers as well as alternative genetic systems in artificial life. In addition, XNA oligomers can be used as biological tools. Acyclic XNAs, which do not have cyclic scaffolds, are attractive due to facile their synthesis and remarkably high nuclease resistance. To maximize the performance of XNAs, a negatively charged backbone is preferable to provide sufficient water solubility; however, acyclic XNAs containing polyanionic backbones suffer from high entropy cost upon duplex formation, because of the high flexibility of the acyclic nature. Herein, we review the relationships between the structure and duplex hybridization properties of various acyclic XNA oligomers with polyanion backbones.

DOI： 10.1002/cbic.202100184

Scopus

PubMed

Dual Crosslinking Photo-Switches for Orthogonal Photo-Control of Hybridization Between Serinol Nucleic Acid and RNA Reviewed

Vol. 27 ( 14 ) page： 4599 - 4604 2021.3

Helical amplification system composed of artificial nucleic acids Reviewed

Kashida, H.; Nishikawa, K.; Shi, W.; Miyagawa, T.; Yamashita, H.; Abe, M.; Asanuma, H.

Chemical Science Vol. 12 ( 5 ) page： 1656 - 1660 2021.2

Nonenzymatic polymerase-like template-directed synthesis of acyclic L-threoninol nucleic acid Reviewed

Murayama, K.; Okita, H.; Kuriki, T.; Asanuma, H.

Nature Communications Vol. 12 page： 804 2021.2

Light-regulated liquid-liquid phase separation for spatiotemporal protein recruitment and cell aggregation Reviewed

Ikeuchi, N.; Komachi, T.; Murayama, K.; Asanuma, H.; Maruyama, A.; Shimada, N.

ACS Applied Materials & Interfaces Vol. 13 ( 4 ) page： 5652 - 5659 2021.2

Quantitative Analyses of Forster Resonance Energy Transfer between Identical Pyrene Chromophores (Homo-FRET) In DNA Scaffolds. Reviewed

Kashida Hiromu, Kawai Hayato, Azuma Hidenori, Araki Yasuyuki, Wada Takehiko, Asanuma Hiroyuki

CHEMPHOTOCHEM Vol. 5 ( 2 ) page： 167 - 172 2021.2

A Pyrene-Modified Serinol Nucleic Acid Nanostructure Converts the Chirality of Threoninol Nucleic Acids into Circularly Polarized Luminescence Signals Reviewed

Kashida H., Nishikawa K., Ito Y., Murayama K., Hayashi I., Kakuta T., Ogoshi T., Asanuma H.

Chemistry - A European Journal 2021

Authorship：Last author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Chemistry - A European Journal

Herein is reported a circularly polarized luminescent (CPL) probe that can respond to the chirality of nucleic acids. An achiral nanostructure was prepared by the hybridization of symmetric serinol nucleic acid (SNA) containing pyrene-modified residues. When chiral oligomers that were complementary to the SNA were added, they induced helicity into the SNA nanowire. Efficient circular dichroism (CD) signal amplification was observed when pyrene was attached to uracil bases through a rigid alkynyl linker. Both CPL and CD signals were observed; they depended on the chirality of the added acyclic threoninol nucleic acid (aTNA) oligomer. This system can be used to convert the chirality of chiral biomolecules into chiroptical signals.

DOI： 10.1002/chem.202102333

Scopus

PubMed

Possibility of pre-RNA world with acyclic XNA before RNA Invited Reviewed

Asanuma Hiroyuki, Okita Hikari, Murayama Keiji

Viva Origino Vol. 49 ( 3 ) page： 9 2021

Language：Japanese Publishing type：Research paper (scientific journal) Publisher：The Society for the Study of the Origin and Evolution of Life Japan

We have developed artificial acyclic nucleic acids composed of serinol derivatives as scaffolds. Recently, template-directed synthesis of acyclic L-threoninol nucleic acid (L-αTNA) could be achieved non-enzymatically via chemical ligation mediated by N-cyanoimidazole in the presence of Mn2+ ion. A pseudo primer extension reaction like natural polymerase was also attained non-enzymatically using a pool of random L-αTNA trimers. In this short review, we discuss the possibility of L-αTNA as a candiate of pre-RNA before RNA world by focusing on the evolution of genetic material.

DOI： 10.50968/vivaorigino.49_9

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Papers - ASANUMA, Hiroyuki