Faculty Profiles - SHIBAYAMA Keigo

Papers - SHIBAYAMA Keigo

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Characterization of three novel Helicobacter species infecting stomachs of dogs and cats: Helicobacter gastrocanis sp. nov., Helicobacter gastrofelis sp. nov., and Helicobacter felistomachi sp. nov. Reviewed

Emiko Rimbara, Sae Aoki, Masato Suzuki, Hirotaka Kobayashi, Taisuke Nakagawa, Yuko Goto-Koshino, Sachiyo Nomura, Wan-Ying Du, Hidenori Matsui, Shigetarou Mori, Keigo Shibayama, Tsuyoshi Kenri, Koichi Ohno

Frontiers in Microbiology 2025.1

A nationwide survey of antimicrobial resistance of Escherichia coli isolated from broiler chickens in Malawi. Reviewed International coauthorship

Chisembe P, Suzuki M, Dao DT, Njunga G, Nkhoma J, Mthilakuwili L, Kinoshita-Daitoku R, Kuroda E, Kimura K, Shibayama K

JAC Antimicrobial Resistance Vol. 6 ( 6 ) 2024.12

Antimicrobial-resistant Helicobacter pylori in Japan: Report of nationwide surveillance for 2018-2020.

Okimoto T, Ando T, Sasaki M, Ono S, Kobayashi I, Shibayama K, Chinda D, Tokunaga K, Nakajima S, Osaki T, Sugiyama T, Kato M, Murakami K

Helicobacter Vol. 29 ( 1 ) page： e13028 2024.1

Characterization of HcaA, a novel autotransporter protein in Helicobacter cinaedi, and its role in host cell adhesion.

Aoki S, Mori S, Matsui H, Shibayama K, Kenri T, Rimbara E

mSphere Vol. 8 ( 6 ) page： e0040323 2023.12

Language：English Publisher：mSphere

Helicobacter cinaedi infects the human gut and causes invasive infections such as bacteremia and cellulitis through bacterial translocation. However, the mechanism by which H. cinaedi attaches to host cells and establishes infection remains unclear. This study aimed to investigate the relationship between a novel putative autotransporter protein, H. cinaedi autotransporter protein A (HcaA), and its role in pathogenicity. The cytotoxicity of H. cinaedi infection in colon epithelial cell lines (Caco-2 and HT29) was assessed using a lactate dehydrogenase assay, and it was found that cytotoxicity significantly decreased upon HcaA knockout. Adhesion assays further revealed that the HcaA-knockout strain showed significantly reduced attachment to the human epithelial colorectal adenocarcinoma (Caco-2) cell line compared to that of the wild-type strain. Moreover, the recombinant HcaA protein demonstrated strong adhesion properties to the human monocytic cell line (U937). The adhesive activity was diminished when the Arg-Gly-Asp (RGD) motif in HcaA was replaced with RAD, indicating that the RGD motif in HcaA is crucial for host cell adhesion. To determine the role of HcaA in H. cinaedi infection in vivo, C57BL/6 mice were orally infected with wild-type and HcaA-knockout H. cinaedi strains. Bacterial colonization was assessed 7, 14, and 28 days post-infection. At 7 days post-infection, colonization was significantly lower in mice infected with the HcaA-knockout strain compared to those infected with the wild-type strain. In conclusion, our findings suggest that HcaA, a novel putative autotransporter protein in H. cinaedi, plays a significant role as an adhesin in establishing colonization.

DOI： 10.1128/msphere.00403-23

Web of Science

Scopus

PubMed

Methicillin-resistant Staphylococcus aureus-induced staphylococcal scalded skin syndrome in a preterm infant, and subsequent toxigenic analysis.

Fukaura R, Terashima-Murase C, Ota M, Noda H, Oka K, Ishihara Y, Shibayama K, Akiyama M

The Journal of dermatology 2023.8

In Vitro Susceptibility of Bacillus cereus Spores of Blood Isolates and Healthy Skin Isolates to 10% Povidone-iodine

ISHIHARA Yuka, SHIBAYAMA Keigo, SHAMOTO Ikue, USAMI Hisae, OHTA Michio

Japanese Journal of Infection Prevention and Control Vol. 38 ( 2 ) page： 57 - 60 2023.3

Language：Japanese Publisher：Japanese Society for Infection Prevention and Control

The susceptibility of Bacillus cereus spores to 10% povidone-iodine was measured at 0°C, 20°C, and 37°C, respectively. The spores of 15 B. cereus blood isolates and 15 isolates from healthy adult skin, respectively, were used for the measurement. The spore solution was prepared 9 days after the culture of B. cereus on NGKG plates, and spore formation was confirmed microscopically. No B. cereus strain in the spore form was highly resistant to any conditions of 10% povidone-iodine treatment. Approximately 99% of the spores of most strains were killed after a 5-min treatment with 10% povidone-iodine irrespective of treatment temperature, whereas 1% of spores survived a 30-min treatment. The spores of 5 blood isolates and 3 healthy skin isolates were more sensitive to 10% povidone-iodine, and only 0.1% of the spores survived the 5-min treatment. No apparent differences in susceptibility to povidone-iodine between the blood and skin isolates were observed. From these results, 5-min treatment using 10% povidone-iodine for skin disinfection is applicable when contamination of B. cereus spores on the skin is expected to be mild or low.

DOI： 10.4058/jsei.38.57

CiNii Research

Rate of Colonization and Serotypes of Streptococcus pneumoniae Isolated from Healthy Adult Community Residents after the Regulatory Approval of PPSV23

ISHIHARA Yuka, YAGI Tetsuya, SHIBAYAMA Keigo, OHTA Michio

Kansenshogaku Zasshi Vol. 96 ( 3 ) page： 82 - 90 2022.5

Language：Japanese Publisher：The Japanese Association for Infectious Diseases

The purpose of this study was to determine the pharyngeal colonization rate and serotypes of Streptococcus pneumoniae in healthy adults in our community by our modified PCR detection method. We set up a new surveillance system for surveying the pharyngeal pneumococcal colonization rate in healthy adults. The pharyngeal colonization rate and the serotypes of S. pneumoniae were investigated in 449 randomly selected healthy adults living in the community between 2014 and 2020, after the launch of the 23-valent pneumococcal polysaccharide vaccine (PPSV23). The 449 examinees included 361 adults aged 65 years or over, who were eligible to receive the PPSV23 vaccine. We performed PCR-based lytA detection using our modified procedure and found pharyngeal S. pneumoniae colonization in 207 examinees (46.1%), a significantly high rate as compared with that reported previously. We then determined the serotypes of the colonizing S. pneumoniae in the examinees by PCR using serotype-specific primers. The PPSV23 serotypes of S. pneumoniae were detected in the pharynx of 188 examinees. Serotype 18C was the most frequently detected, and serotypes 14 and 4 were also detected at a high frequency. The colonization rate of S. pneumoniae belonging to the PCV7 serotypes was 67.6%, while the rate for the PCV13 serotypes was 73.4%. Since the colonization rate of S. pneumoniae belonging to the PPSV23 serotypes was 88.9%, PPSV23 as well as PCV13 were found to be applicable to elderly people in the district.Of the elderly examinees age 65 or over, 53.5% had received the PPSV23 vaccine before this study. The pharyngeal colonization rate of S. pneumoniae in the group that had already received the PPSV23 vaccine was 38.9%, whereas that in the group that had not received the PPSV23 vaccine was 49.4%. The colonization rate was not influenced by prior influenza vaccination or the contact frequency of the elderly examinees with their grandchildren. Conclusion; We found a much higher rate, as compared with previous reports, of pharyngeal S. pneumoniae colonization in local community-dwelling adults using our modified PCR method. We also found no apparent serotype changes in the S. pneumoniae colonizing the pharynx in local community-dwelling adults after the introduction of the PCV13 or PPSV23 vaccine.

DOI： 10.11150/kansenshogakuzasshi.96.82

CiNii Research

Surveillance of multi-drug resistance phenotypes in Staphylococcus aureus in Japan and correlation with whole-genome sequence findings. Open Access

Hosaka Y, Yahara K, Clark A, Kitagawa H, Hisatsune J, Sugai M, Shibayama K, Stelling J

The Journal of hospital infection Vol. 123 page： 34 - 42 2022.5

Language：English Publisher：Journal of Hospital Infection

Background: Antimicrobial resistance in Staphylococcus aureus imposes a high disease burden. Both phenotypic and genotypic monitoring are key to understanding and containing emerging resistant strains. Aim: Phenotypic monitoring of emerging resistance in S. aureus and correlation of priority strain phenotypes with whole-genome sequencing (WGS) findings. Methods: Antimicrobial susceptibility test results of >40,000 isolates from 213 participating hospitals from 2011 to 2019 were exported from the national Japan Nosocomial Infections Surveillance (JANIS) database. Longitudinal and geographic distribution and prevalence of distinct multi-drug resistance phenotypes (‘resistance profiles’) of S. aureus were examined among hospitals and prefectures. We further conducted a genome sequence analysis of strains with specific resistance profiles of concern. Findings: The overall prevalence of meticillin-resistant S. aureus (MRSA) decreased from 40.3% to 35.1% from 2011 to 2019. However, among dozens of S. aureus resistance profiles, only one profile of a type of MRSA, exhibited a statistically significant increase in inpatient frequency, exceeding 10% during the nine years. This MRSA profile showed resistance to oxacillin, erythromycin and levofloxacin. Analysis of WGS results of S. aureus isolates with this phenotype revealed that most belonged to clonal complex 8, and all carried SCCmec IV, typical of community-acquired MRSA. Conclusion: Tracking distinct resistance profiles deepened our understanding of the overall decrease in MRSA and led to recognition of the emergence of a new resistance phenotype. This study provides a model for future epidemiological research on antimicrobial resistance correlating multi-drug resistance phenotypes with selective genome sequencing, which can be applied to other bacterial species.

DOI： 10.1016/j.jhin.2022.02.011

Emergence of mobile tigecycline resistance gene tet(X4)-harbouring Shewanella xiamenensis in a water environment

Trung Duc Dao, Kasuga Ikuro, Hirabayashi Aki, Dong Tu Nguyen, Hien Thi Tran, Vu Hieu, Linh Tuyet Ngoc Pham, Thi My Hanh Vu, Hasebe Futoshi, Ha Thanh Nguyen, Trang Le Thi, Tran Hoang Huy, Shibayama Keigo, Takemura Taichiro, Suzuki Masato

JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE Vol. 28 page： 140 - 142 2022.3

Helicobacter suis SNTW101株の培養成功と完全ゲノム配列の決定

林原絵美子, 松井英則, 鈴木仁人, 中村正彦, 柴山恵吾, 徳永健吾

日本ヘリコバクター学会学術集会プログラム・抄録集 Vol. 26回 page： 58 - 58 2020.12

Helicobacter suis胃粘膜感染と病態発症

鈴木仁人, 林原絵美子, 松井英則, 徳永健吾, 柴山恵吾

日本ヘリコバクター学会学術集会プログラム・抄録集 Vol. 26回 page： 73 - 73 2020.12

胃MALTリンパ腫におけるHelicobacter suis感染の関与

徳永健吾, 林原絵美子, 松井英則, 鈴木仁人, 大崎敬子, 井田陽介, 三好佐和子, 長濱清隆, 大野亜希子, 三好潤, 柴山恵吾, 久松理一, 岡本晋

Gastroenterological Endoscopy Vol. 62 ( Suppl.2 ) page： 2101 - 2101 2020.10

FOCUS 輸入感染症-耐性菌を中心に

柴山恵吾

検査と技術 Vol. 48 ( 7 ) page： 684 - 687 2020.7

特集薬剤耐性(AMR)対策厚生労働省院内感染対策サーベイランス(JANIS)からみたAMR対策の課題と展望

柴山恵吾

公衆衛生 Vol. 81 ( 10 ) page： 798 - 803 2017.10

Expanding distributions of red back spiders and bites in Japan from 2011 to 2013

Yamagishi Takuya, Arai Satoru, Hifumi Toru, Sawabe Kyoko, Yamamoto Akihiko, Shibayama Keigo, Ato Manabu, Taki Hisashi, Goka Koichi, Oishi Kazunori

Medical Entomology and Zoology Vol. 67 ( 4 ) page： 219 - 221 2016

Language：English Publishing type：Research paper (scientific journal) Publisher：The Japan Society of Medical Entomology and Zoology

In Japan, fourteen bites by the red back spider, Latrodectus hasseltii, were reported in four hospitals between 2011 and 2013 in a survey of sentinel hospitals. The distribution of the spider and the areas in which patients were bitten by the spider both expanded geographically each year. Although fatalities or severely ill patients are yet to be reported in Japan, stockpiles of the antivenom and communication with the public and medical professionals should be considered.

DOI： 10.7601/mez.67.219

CiNii Research

Understanding Procedural Knowledge for Solving Arithmetic Task by Externalization

Miwa, K; Terai, H; Shibayama, K

INTELLIGENT TUTORING SYSTEMS, ITS 2016 Vol. 9684 page： 3 - 12 2016

Language：English Publisher：Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)

Students build cognitive models for solving a cryptarithmetic task in a learning environment that enables them to formally describe various types of procedural knowledge in a group learning setting in which each student is allowed to refer to the procedural rules described by the other group members. Experimental evaluation showed that: (1) three-quarters of participants successfully constructed valid models with the system, and (2) participants learned to describe procedural knowledge more precisely not only for the training task (cryptarithmetic task) but also for a transfer task (bug identification for a multi-column subtraction problem).

DOI： 10.1007/978-3-319-39583-8_1

Web of Science

Scopus

アンチバイオグラム

柴山恵吾

ファルマシア Vol. 51 ( 4 ) page： 342_2 - 342_2 2015

Language：Japanese Publishing type：Research paper (scientific journal) Publisher：公益社団法人日本薬学会

国，地域，病院，または病棟ごとに分離された細菌の薬剤感受性試験のデータを集計し，それぞれの菌種で各種抗菌薬について耐性，中間，感性の菌がどれくらいの割合であるのかを図や表にしたもの．薬剤耐性菌対策を検討していくための重要な基本情報となる．また，臨床現場における治療薬のガイドラインを作成する際には，重要な参考情報となる．さらに，病院や病棟ごとでデータの経時的な変化を見たり，そのデータを地域や国全体のデータと比較することで，感染制御がうまくできているかどうかを評価することにも用いられる．

DOI： 10.14894/faruawpsj.51.4_342_2

CiNii Research

日本における薬剤耐性菌の状況

柴山恵吾

ファルマシア Vol. 51 ( 4 ) page： 324 - 328 2015

Language：Japanese Publishing type：Research paper (scientific journal) Publisher：公益社団法人日本薬学会

20世紀に入ってからペニシリンをはじめとする様々な抗菌薬が開発され，人類にとって大きな脅威である感染症から多くの人が救われてきた．しかし一方で，新たな抗菌薬が開発されると間もなく，その薬剤に耐性を獲得した細菌が出現し，薬剤の普及とともに拡散してきた．これまでに耐性が出現しなかった抗菌薬はなく，これは今後も続くと考えられる．近年，薬剤耐性菌は国境を越えて拡散し，世界的な問題になっている．菌種や薬剤によっては，耐性菌の割合が数年で大きく増加しているものもある．WHOは2013年に薬剤耐性菌に関する諮問グループを組織し，2014年に薬剤耐性菌に関する報告書を出した．WHOは，この中で今後取り組むべき課題の1つとして，薬剤耐性菌のグローバルサーベイランスの強化を挙げている． 日本においては，薬剤耐性菌のサーベイランスとして厚生労働省院内感染対策サーベイランス（Japan Nosocomial Infection Surveillance：JANIS）事業がある．本稿では，JANISの2013年の公開情報2）のデータを用いて，主要な各種耐性菌の国内での状況を紹介する．さらに，近年特に問題になっているカルバペネム耐性の腸内細菌科細菌について，日本でよく検出される耐性遺伝子や外国の状況との比較なども説明する．また，JANISが各参加医療機関個別に感染対策に活用していただくことを目的に返している報告書についても触れる．

DOI： 10.14894/faruawpsj.51.4_324

CiNii Research

28-P2PM-017 本邦におけるAcinetobacter spp.の分離状況と耐性獲得状況(感染制御(その他)1,一般演題(ポスター),新時代を拓く医療薬学フロンティア)

木村修徳, 松井真理, 鈴木里和, 鈴木仁人, 柴山恵吾, 三浦布紗子, 永野真久, 安森奈緒子, 井上大奨, 平木洋一, 真鍋健一, 河野文夫

日本医療薬学会年会講演要旨集 Vol. 24 ( 0 ) page： 421 2014.8

Genome sequence of a strain of the human pathogenic bacterium Pseudomonas alcaligenes that caused bloodstream infection Reviewed

Suzuki M., Suzuki S., Matsui M., Hiraki Y., Kawano F., Shibayama K.

Genome Announcements Vol. 1 ( 5 ) 2013

Language：English Publishing type：Research paper (scientific journal) Publisher：Genome Announcements

Pseudomonas alcaligenes, a Gram-negative aerobic bacterium, is a rare opportunistic human pathogen. Here, we report the whole-genome sequence of P. alcaligenes strain MRY13-0052, which was isolated from a bloodstream infection in a medical institution in Japan and is resistant to antimicrobial agents, including broad-spectrum cephalosporins and monobactams.

DOI： 10.1128/genomeA.e00919-13

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Papers - SHIBAYAMA Keigo