Faculty Profiles - SHIBAYAMA Keigo

Papers - SHIBAYAMA Keigo

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A preliminary survey of extended-spectrum β-lactamases (ESBLs) in clinical isolates of Klebsiella pneumoniae and Escherichia coli in Japan Reviewed

Tetsuya Yagi, Hiroshi Kurokawa, Naohiro Shibata, Keigo Shibayama, Yoshichika Arakawa

FEMS Microbiology Letters Vol. 184 ( 1 ) page： 53 - 56 2000.3

Language：English Publishing type：Research paper (scientific journal)

We conducted a survey of extended-spectrum β-lactamases (ESBLs) among 16 805 Escherichia coli and 9794 Klebsiella pneumoniae clinical isolates recovered from 196 separate medical institutions during the period January 1997 to January 1998. Using the criteria for minimal inhibitory concentrations (MICs) of oxyimino-cephalosporins of ≥8 μg ml-1 and confirmation by double-disk test, we detected 15 E. coli and 34 K. pneumoniae isolates producing ESBLs. Genotypes of ESBLs determined by PCR with type- specific primers included one TEM-derived and 24 SHV-derived ESBLs, in addition to 24 Toho-1-type ESBLs, one of the major types of ESBLs reported in Japan. Nucleotide sequence analysis of SHV-specific PCR products revealed that SHV-12 was the dominant type of SHV-derived ESBL. In addition, we also identified TEM-26 and SHV-2. This is the first report characterizing TEM- and SHV-derived ESBLs in Japan. (C) 2000 Published by Elsevier Science B.V.

DOI： 10.1016/S0378-1097(00)00016-1

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Medium compositions and culture conditions for the assay of fosfomycin susceptibility by Etest

Toshinobu Horii, Taku Kimura, Takuya Odagiri, Keigo Shibayama, Michio Ohta

Journal of Infection and Chemotherapy Vol. 6 ( 1 ) page： 30 - 34 2000

Language：English Publishing type：Research paper (scientific journal) Publisher：Springer Japan

The Etest (AB Biodisk, Solna, Sweden), a susceptibility testing method, was used for fosfomycin and was evaluated by comparison with the agar dilution method for 73 clinical isolates of Escherichia coli, including two resistant strains, and an optimal Etest method for fosfomycin was determined. Media and culture conditions greatly affected the minimum inhibitory concentrations (MICs) determined by the Etest for fosfomycin, as shown for the agar dilution methods. Our results showed that the most favorable conditions for the Etest for fosfomycin were with Mueller-Hinton agar (Becton-Dickinson Japan, Tokyo, Japan) under aerobic conditions. However, the MICs for the resistant strains were much higher than those determined by agar dilution methods, using Nutrient agar (Becton-Dickinson Japan) under anaerobic conditions. The addition of glucose-6-phosphate did not significantly affect the Etest results.

DOI： 10.1007/s101560050046

A New SHV-derived extended-spectrum β-lactamase (SHV-24) that hydrolyzes ceftazidime through a single-amino-acid substitution (D179G) in the Ω-loop Reviewed

Hiroshi Kurokawa, Tetsuya Yagi, Naohiro Shibata, Keigo Shibayama, Kazunari Kamachi, Yoshichika Arakawa

Antimicrobial Agents and Chemotherapy Vol. 44 ( 6 ) page： 1725 - 1727 2000

Language：English Publishing type：Research paper (scientific journal)

A new SHV-derived extended-spectrum β-lactamase (SHV-24) conferring high-level resistance to ceftazidime but not cefotaxime and cefazolin was identified in Japan. This enzyme was encoded by a transferable 150-kb plasmid from an Escherichia coli clinical isolate. The pI and K(m) for CAZ of this enzyme were 7.5 and 30 μM, respectively. SHV-24 was found to have a D179G substitution in the Ω-loop of the enzyme.

DOI： 10.1128/AAC.44.6.1725-1727.2000

Convenient test for screening metallo-β-lactamase-producing gram- negative bacteria by using thiol compounds Reviewed

Yoshichika Arakawa, Naohiro Shibata, Keigo Shibayama, Hiroshi Kurokawa, Tetsuya Yagi, Hiroshi Fujiwara, Masafumi Goto

Journal of Clinical Microbiology Vol. 38 ( 1 ) page： 40 - 43 2000

Language：English Publishing type：Research paper (scientific journal)

A simple disk diffusion test was constructed for detection of IMP-1-type metallo-β-lactamase-producing gram-negative bacteria. Two Kirby-Bauer disks containing ceftazidime (CAZ) and a filter disk containing a metallo-β- lactamase inhibitor were used in this test. Several IMP-1 inhibitors such as thiol compounds including 2-mercaptopropionic acid, heavy metal salts, and EDTA were evaluated for this test. Two CAZ disks were placed on a Mueller- Hinton agar plate on which a bacterial suspension was spread according to the method recommended by the National Committee for Clinical Laboratory Standards. The distance between the disks was kept to about 4 to 5 cm, and a filter disk containing a metallo-β-lactamase inhibitor was placed near one of the CAZ disks within a center-to-center distance of 1.0 to 2.5 cm. For IMP-1-producing strains, the growth-inhibitory zone between the two disks expanded, while no evident change in the shape of the growth-inhibitory zone was observed for CAZ-resistant strains producing serine β-lactamases such as AmpC or SHV-12. As a result, 2 to 3 μl of undiluted 2-mercaptopropionic acid or mercaptoacetic acid able to block IMP-1 activity gave the most reproducible and clearest results, and CAZ-resistant strains producing AmpC or extended-spectrum β-lactamases were distinguishable from IMP-1 producers by this test. A similar observation was made with IMP-1-producing clinical isolates such as Serratia marcescens, Klebsiella pneumoniae, Escherichia coli, Enterobacter cloacae, Enterobacter aerogenes, Citrobacter freundii, Proteus vulgaris, Pseudomonas aeruginosa, Pseudomonas putida, Acinetobacter spp., and Alcaligenes xylosoxidans. The specificity and sensitivity of this test were comparable to those of PCR analysis using bla(IMP)-specific primers. Therefore, this convenient test would be valuable for daily use in clinical laboratories.

DOI： 10.1128/JCM.38.1.40-43.2000

Role of multiple efflux pumps in Escherichia coli in indole expulsion Reviewed

Kawamura-Sato K, Shibayama K, Horii T, Iimuma Y, Arakawa Y, Ohta M

FEMS MICROBIOLOGY LETTERS Vol. 179 ( 2 ) page： 345 - 352 1999.10

Worldwide proliferation of carbapenem-resistant gram-negative bacteria Reviewed

Kurokawa H, Yagi T, Shibata N, Shibayama K, Arakawa Y

LANCET Vol. 354 ( 9182 ) page： 955 - 955 1999.9

Worldwide proliferation of carbapenem-resistant gram-negative bacteria [9] Reviewed

H. Kurokawa, T. Yagi, N. Shibata, K. Shibayama, Y. Arakawa

Lancet Vol. 354 ( 9182 ) page： 955 - 955 1999.9

Streptococcal pyrogenic exotoxin F (SpeF) causes permeabilization of lung blood vessels Reviewed

Matsumoto M, Ishikawa N, Saito M, Shibayama K, Horii T, Sato K, Ohta M

INFECTION AND IMMUNITY Vol. 67 ( 9 ) page： 4307 - 4311 1999.9

Four critical aspartic acid residues potentially involved in the catalytic mechanism of Escherichia coli K-12 WaaR Reviewed

Shibayama K, Ohsuka S, Sato K, Yokoyama K, Horii T, Ohta M

FEMS MICROBIOLOGY LETTERS Vol. 174 ( 1 ) page： 105 - 109 1999.5

Pathological effect of synthetic cereulide, an emetic toxin of Bacillus cereus, is reversible in mice Reviewed

Keiko Yokoyama, Masafumi Ito, Norio Agata, Minoru Isobe, Keigo Shibayama, Toshinobu Horii, Michio Ohta

FEMS Immunology and Medical Microbiology Vol. 24 ( 1 ) page： 115 - 120 1999.5

Language：English Publishing type：Research paper (scientific journal)

Cereulide is the causative toxin of the emetic type of food-borne illness caused by Bacillus cereus. This toxin was previously shown to be associated with fulminant liver failure in a human case. Mice were injected i.p. with synthetic cereulide and the development of histopathological changes was examined. Hepatocytes showed mitochondrial swelling with loss of cristae, and dose-dependent increase of small fatty droplets. These microsteatotic hepatocytes were distributed mainly in the pericentral area. At higher cereulide doses, massive degeneration of hepatocytes occurred. The serum values of hepatic enzymes were highest on days 2-3 after the inoculation of cereulide, and rapidly decreased thereafter. General recovery from the pathological changes and regeneration of hepatocytes was observed after 4 weeks. Copyright (C) 1999 Federation of European Microbiological Societies.

DOI： 10.1016/S0928-8244(99)00017-6

Emergence of fosfomycin-resistant isolates of shiga-like toxin-producing Escherichia coli O26 Reviewed

Horii T, Kimura T, Sato K, Shibayama K, Ohta M

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY Vol. 43 ( 4 ) page： 789 - 793 1999.4

Antibacterial activities of new synthetic divalent cation chelators Reviewed

Ashoori M, Ohta M, Ohsuka S, Shibayama K, Horii T, Ueda M, Kurosaki H

MICROBIOLOGY AND IMMUNOLOGY Vol. 43 ( 4 ) page： 311 - 316 1999

Are autoantibodies against Lewis antigens involved in the pathogenesis of Helicobacter pylori-induced peptic ulcers? Reviewed

Kamiya K, Arisawa T, Goto H, Shibayama K, Horii T, Hayakawa T, Ohta M

MICROBIOLOGY AND IMMUNOLOGY Vol. 43 ( 5 ) page： 403 - 408 1999

Growth conditions of and emetic toxin production by Bacillus cereus in a defined medium with amino acids Reviewed

Agata N, Ohta M, Mori M, Shibayama K

MICROBIOLOGY AND IMMUNOLOGY Vol. 43 ( 1 ) page： 15 - 18 1999

Conserved structural regions involved in the catalytic mechanism of Escherichia coli K-12 WaaO (RfaI) Reviewed

Shibayama K, Ohsuka S, Tanaka T, Arakawa Y, Ohta M

JOURNAL OF BACTERIOLOGY Vol. 180 ( 20 ) page： 5313 - 5318 1998.10

Heterogeneity of phenotypic and genotypic traits including organic-acid resistance in Escherichia coli O157 isolates

T Horii, S Barua, T Kimura, S Kasugai, K Sato, K Shibayama, S Ichiyama, M Ohta

MICROBIOLOGY AND IMMUNOLOGY Vol. 42 ( 12 ) page： 871 - 874 1998

Language：English Publishing type：Research paper (scientific journal) Publisher：CENTER ACADEMIC PUBL JAPAN

We undertook an epidemiologic study for the sensitivity of both Shiga-like toxin (Slt)-producing Escherichia coli (STEC) O157 and non-STEC O157 strains isolated from different patients with diarrhea to hydrochloric acid (HCl) and organic acids such as acetate, propionate, butyrate and lactate, and other pathogenic factors. The E. coli O157 isolates examined showed a wide variety of organic-acid susceptibility patterns. E. coli O157 isolates resistant to HCl or acetate were found more frequently than those resistant to other organic acids. These isolates also showed diverse pathogenicity patterns for the presence of the virulence genes, antibiotic susceptibility and plasmid profile.

DOI： 10.1111/j.1348-0421.1998.tb02363.x

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Nosocomial spread of cephem-resistant Escherichia coli strains carrying multiple toho-1-like beta-lactamase genes Reviewed

Yagi T, Kurokawa H, Senda K, Ichiyama S, Ito H, Ohsuka S, Shibayama K, Shimokata K, Kato N, Ohta M, Arakawa Y

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY Vol. 41 ( 12 ) page： 2606 - 2611 1997.12

RobA-induced multiple antibiotic resistance largely depends on the activation of the AcrAB efflux Reviewed

Tanaka T, Horii T, Shibayama K, Sato K, Ohsuka S, Arakawa Y, Yamaki K, Takagi K, Ohta M

MICROBIOLOGY AND IMMUNOLOGY Vol. 41 ( 9 ) page： 697 - 702 1997

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Papers - SHIBAYAMA Keigo