Faculty Profiles - SHIBAYAMA Keigo

Papers - SHIBAYAMA Keigo

Division display 201 - 220 of about 318 ／ All the affair displays >>

Pararenal Lymphatic Cyst Infection Caused by Helicobacter cinaedi. Reviewed

Yusaku Akashi, Jun Igarashi, Hiromichi Suzuki, Emiko Rimbara, Keigo Shibayama, Sayaka Nin, Kiyoko Tamai, Yuji Yaguchi, Masanari Shiigai, Takehiro Oikawa, Masatsune Suzuki

Internal medicine (Tokyo, Japan) Vol. 54 ( 11 ) page： 1437 - 40 2015

Language：English Publishing type：Research paper (scientific journal) Publisher：The Japanese Society of Internal Medicine

A 43-year-old man was referred to our hospital for an acute-onset fever and left flank pain. He had been previously diagnosed with lymphangioma, and abdominal computed tomography showed pararenal cysts with fat stranding around the left kidney, of which infection was subsequently confirmed on magnetic resonance imaging. Gram-negative spiral bacilli were isolated from two sets of blood cultures, and Helicobacter cinaedi was identified using 16S rRNA sequencing. The patient was successfully treated with ceftriaxone therapy without recurrence. A multilocus sequence typing analysis indicated the current H. cinaedi strain differed from previous strains isolated in Japan.

DOI： 10.2169/internalmedicine.54.3991

Roles of Ala-149 in the catalytic activity of diadenosine tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv. Reviewed International journal

Shigetarou Mori, Hyun Kim, Emiko Rimbara, Yoshichika Arakawa, Keigo Shibayama

Bioscience, biotechnology, and biochemistry Vol. 79 ( 2 ) page： 236 - 8 2015

Language：English Publishing type：Research paper (scientific journal)

Diadenosine 5',5'''-P(1),P(4)-tetraphosphate (Ap4A) phosphorylase from Mycobacterium tuberculosis H37Rv (MtAPA) belongs to the histidine triad motif (HIT) superfamily, but is the only member with an alanine residue at position 149 (Ala-149). Enzymatic analysis revealed that the Ala-149 deletion mutant displayed substrate specificity for diadenosine 5',5'''-P(1),P(5)-pentaphosphate and was inactive on Ap4A and other substrates that are utilized by the wild-type enzyme.

DOI： 10.1080/09168451.2014.973364

Venomous snake bites: clinical diagnosis and treatment. Reviewed International journal

Toru Hifumi, Atsushi Sakai, Yutaka Kondo, Akihiko Yamamoto, Nobuya Morine, Manabu Ato, Keigo Shibayama, Kazuo Umezawa, Nobuaki Kiriu, Hiroshi Kato, Yuichi Koido, Junichi Inoue, Kenya Kawakita, Yasuhiro Kuroda

Journal of intensive care Vol. 3 ( 1 ) page： 16 - 16 2015

Language：English Publishing type：Research paper (scientific journal)

Snake bites are life-threatening injuries that can require intensive care. The diagnosis and treatment of venomous snake bites is sometimes difficult for clinicians because sufficient information has not been provided in clinical practice. Here we review the literature to present the proper management of bites by mamushi, habu, and yamakagashi snakes, which widely inhabit Japan and other Asian countries. No definite diagnostic markers or kits are available for clinical practice; therefore, definitive diagnosis of snake-venom poisoning requires positive identification of the snake and observation of the clinical manifestations of envenomation. Mamushi (Gloydius blomhoffii) bites cause swelling and pain that spreads gradually from the bite site. The platelet count gradually decreases due to the platelet aggregation activity of the venom and can decrease to <100,000/mm(3). If the venom gets directly injected into the blood vessel, the platelet count rapidly decreases to <10,000/mm(3) within 1 h after the bite. Habu (Protobothrops flavoviridis) bites result in swelling within 30 min. Severe cases manifest not only local signs but also general symptoms such as vomiting, cyanosis, loss of consciousness, and hypotension. Yamakagashi (Rhabdophis tigrinus) bites induce life-threatening hemorrhagic symptoms and severe disseminated intravascular coagulation with a fibrinolytic phenotype, resulting in hypofibrinogenemia and increased levels of fibrinogen degradation products. Previously recommended first-aid measures such as tourniquets, incision, and suction are strongly discouraged. Once airway, breathing, and circulation have been established, a rapid, detailed history should be obtained. If a snake bite is suspected, hospital admission should be considered for further follow-up. All venomous snake bites can be effectively treated with antivenom. Side effects of antivenom should be prevented by sufficient preparation. Approved antivenoms for mamushi and habu are available. Yamakagashi antivenom is used as an off-label drug in Japan, requiring clinicians to join a clinical research group for its use in clinical practice.

DOI： 10.1186/s40560-015-0081-8

First Report of Metallo-beta-Lactamase NDM-5-Producing Escherichia coli in Japan Reviewed

Ryuichi Nakano, Akiyo Nakano, Kenji Hikosaka, Sayoko Kawakami, Naohisa Matsunaga, Miwa Asahara, Shinobu Ishigaki, Taiji Furukawa, Masato Suzuki, Keigo Shibayama, Yasuo Ono

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY Vol. 58 ( 12 ) page： 7611 - 7612 2014.12

An outbreak of bla(OXA-51-like)(-) and bla(OXA-66)(-) positive Acinetobacter baumannii ST208 in the emergency intensive care unit Reviewed

Satomi Asai, Kazuo Umezawa, Hideo Iwashita, Toshio Ohshima, Maya Ohashi, Mika Sasaki, Hideki Hayashi, Mari Matsui, Keigo Shibayama, Sadaki Inokuchi, Hayato Miyachi

JOURNAL OF MEDICAL MICROBIOLOGY Vol. 63 ( Pt 11 ) page： 1517 - 1523 2014.11

Language：English Publishing type：Research paper (scientific journal) Publisher：SOC GENERAL MICROBIOLOGY

A series of clinical isolates of drug-resistant (DR) Acinetobacter baumannii with diverse drug susceptibility was detected from eight patients in the emergency intensive care unit of Tokai University Hospital. The initial isolate was obtained in March 2010 (A. baumannii Tokai strain 1); subsequently, seven isolates were obtained from patients (A. baumannii Tokai strains 2-8) and one isolate was obtained from an air-fluidized bed used by five of the patients during the 3 months from August to November 2011. The isolates were classified into three types of antimicrobial drug resistance patterns (RRR, SRR and SSR) according to their susceptibility (S) or resistance (R) to imipenem, amikacin and ciprofloxacin, respectively. Genotyping of these isolates by multilocus sequence typing revealed one sequence type, ST208, whilst that by a DiversiLab analysis revealed two subtypes. All the isolates were positive for bla(OXA-51-like) and bla(OXA-66), as assessed by PCR and DNA sequencing. A. baumannii Tokai strains 1-8 and 10 (RRR, SRR and SSR) had quinolone resistance-associated mutations in gyrA/parC, as revealed by DNA sequencing. The ISAba / upstream of bla(OXA-51-like) and aminoglycoside resistance-associated gene, armA, were detected in A. baumannii Tokai strains 1-7 and 10 (RRR and SRR) as assessed by PCR. Among the genes encoding resistance nodulation division family pumps (adeB, adeG and adeJ) and outer-membrane porins (oprD and carO) overexpression of adeB and adeJ and suppression of oprD and car were seen in isolates of A. baumannii Tokai strain 2 (RRR), as assessed by realtime PCR. Thus, the molecular characterization of a series of isolates of DR A. baumannii revealed the outbreak of ST208 and diverse antimicrobial drug susceptibilities, which almost correlated with differential gene alterations responsible for each type of drug resistance.

DOI： 10.1099/jmm.0.077503-0

Genetic Characterization and Comparison of Clostridium botulinum Isolates from Botulism Cases in Japan between 2006 and 2011 Reviewed

Tsuyoshi Kenri, Tsuyoshi Sekizuka, Akihiko Yamamoto, Masaaki Iwaki, Takako Komiya, Takashi Hatakeyama, Hiroshi Nakajima, Motohide Takahashi, Makoto Kuroda, Keigo Shibayama

APPLIED AND ENVIRONMENTAL MICROBIOLOGY Vol. 80 ( 22 ) page： 6954 - 6964 2014.11

Language：English Publishing type：Research paper (scientific journal) Publisher：AMER SOC MICROBIOLOGY

Genetic characterization was performed for 10 group I Clostridium botulinum strains isolated from botulism cases in Japan between 2006 and 2011. Of these, 1 was type A, 2 were type B, and 7 were type A(B) {carrying a silent bont/B [bont/(B)] gene} serotype strains, based on botulinum neurotoxin (BoNT) production. The type A strain harbored the subtype A1 BoNT gene (bont/ A1), which is associated with the ha gene cluster. The type B strains carried bont/B5 or bont/B6 subtype genes. The type A(B) strains carried bont/A1 identical to that of type A(B) strain NCTC2916. However, bont/(B) genes in these strains showed single-nucleotide polymorphisms (SNPs) among strains. SNPs at 2 nucleotide positions of bont/(B) enabled classification of the type A(B) strains into 3 groups. Pulsed-field gel electrophoresis (PFGE) and multiple-locus variable-number tandem-repeat analysis (MLVA) also provided consistent separation results. In addition, the type A(B) strains were separated into 2 lineages based on their plasmid profiles. One lineage carried a small plasmid (5.9 kb), and another harbored 21-kb plasmids. To obtain more detailed genetic information about the 10 strains, we sequenced their genomes and compared them with 13 group I C. botulinum genomes in a database using whole-genome SNP analysis. This analysis provided high-resolution strain discrimination and enabled us to generate a refined phylogenetic tree that provides effective traceability of botulism cases, as well as bioterrorism materials. In the phylogenetic tree, the subtype B6 strains, Okayama2011 and Osaka05, were distantly separated from the other strains, indicating genomic divergence of subtype B6 strains among group I strains.

DOI： 10.1128/AEM.02134-14

Reverse transcription polymerase chain reaction-based method for selectively detecting vegetative cells of toxigenic Clostridium difficile Reviewed

Mitsutoshi Senoh, Haru Kato, Tomoko Murase, Hideharu Hagiya, Yasuaki Tagashira, Tadashi Fukuda, Masaaki Iwaki, Akihiko Yamamoto, Keigo Shibayama

MICROBIOLOGY AND IMMUNOLOGY Vol. 58 ( 11 ) page： 615 - 620 2014.11

Language：English Publishing type：Research paper (scientific journal) Publisher：WILEY-BLACKWELL

The laboratory diagnostic methods for Clostridium difficile infection (CDI) include toxigenic culture, enzyme immunoassays (EIAs) to detect the toxins of C. difficile, and nucleic acid amplification tests (NAATs) to detect C. difficile toxin genes, but each of these methods has disadvantages; toxigenic cultures require a long time to produce results, EIAs have low sensitivity, and NAATs that target DNA cannot distinguish vegetative cells from spores and dead cells. Here we report a new detection method that uses reverse transcription polymerase chain reaction to target the toxin-gene transcripts. This method was able to specifically detect the vegetative cells of toxigenic C. difficile in fecal samples in spike tests, with a minimum detection limit of 5x10(2) colony-forming units per 100mg of stool specimen. The performance of this method was also demonstrated in a pilot scale evaluation using clinical fecal specimens, which showed that this method may be more sensitive than EIA and requires a shorter time than toxigenic culture. This method could potentially be applied in the clinical laboratory to detect C. difficile in fecal specimens. The ability of this method to discriminate the presence of vegetative cells from spores and dead cells could help to further the understanding of CDI.

DOI： 10.1111/1348-0421.12189

Loop-mediated isothermal amplification assay for 16S rRNA methylase genes in Gram-negative bacteria Reviewed

Mitsuaki Nagasawa, Mitsuo Kaku, Kazunari Kamachi, Keigo Shibayama, Yoshichika Arakawa, Keizo Yamaguchi, Yoshikazu Ishii

JOURNAL OF INFECTION AND CHEMOTHERAPY Vol. 20 ( 9-10 ) page： 635 - 638 2014.9

Language：English Publishing type：Research paper (scientific journal) Publisher：SPRINGER JAPAN KK

Using the loop-mediated isothermal amplification (LAMP) method, we developed a rapid assay for detection of 16S rRNA methylase genes (rmtA, rmtB, and armA), and investigated 16S rRNA methylase-producing strains among clinical isolates. Primer Explorer V3 software was used to design the LAMP primers. LAMP primers were prepared for each gene, including two outer primers (F3 and B3), two inner primers (FIP and BIP), and two loop primers (LF and LB). Detection was performed with the Loopamp DNA amplification kit. For all three genes (rmtA, rmtB, and armA), 10(2) copies/tube could be detected with a reaction time of 60 mm. When nine bacterial species (65 strains saved in National Institute of Infectious Diseases) were tested, which had been confirmed to possess rmtA, rmtB, or armA by PCR and DNA sequencing, the genes were detected correctly in these bacteria with no false negative or false positive results. Among 8447 clinical isolates isolated at 36 medical institutions, the LAMP method was conducted for 191 strains that were resistant to aminoglycosides based on the results of antimicrobial susceptibility tests. Eight strains were found to produce 16S rRNA methylase (0.09%), with rmtB being identified in three strains (0.06%) of 4929 isolates of Enterobacteriaceae, rmtA in three strains (0.10%) of 3284 isolates of Pseudomonas aeruginosa, and armA in two strains (0.85%) of 234 isolates of Acinetobacter spp. At present, the incidence of strains possessing 16S rRNA methylase genes is very low in Japan. However, when Gram-negative bacteria showing high resistance to aminoglycosides are isolated by clinical laboratories, it seems very important to investigate the status of 16S rRNA methylase gene-harboring bacilli and monitor their trends among Japanese clinical settings. (C) 2014, Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.jiac.2014.08.013

Molecular characterization of nicotinate phosphoribosyltransferase from Mycobacterium tuberculosis H37Rv, and inhibition of its activity by pyrazinoic acid Reviewed

Mori S., Kim H., Rimbara E., Arakawa Y., Shibayama K.

FEBS JOURNAL Vol. 281 page： 584 - 585 2014.9

Penicillin-Susceptible Group B Streptococcal Clinical Isolates with Reduced Cephalosporin Susceptibility Reviewed

Noriyuki Nagano, Yukiko Nagano, Masami Toyama, Kouji Kimura, Keigo Shibayama, Yoshichika Arakawa

JOURNAL OF CLINICAL MICROBIOLOGY Vol. 52 ( 9 ) page： 3406 - 3410 2014.9

Language：English Publishing type：Research paper (scientific journal) Publisher：AMER SOC MICROBIOLOGY

We characterized penicillin-susceptible group B streptococcal (PSGBS) clinical isolates exhibiting no growth inhibition zone around a ceftibuten disk (CTBr PSGBS). The CTBr PSGBS isolates, for which augmented MICs of cefaclor and ceftizoxime were found, shared a T394A substitution in penicillin-binding protein 2X (PBP 2X) and a T567I substitution in PBP 2B, together with an additional G429S substitution in PBP 2X or a T145A substitution in PBP 1A, although the T145A substitution in the transglycosidase domain of PBP 1A would have no effect on the level of resistance to ceftibuten.

DOI： 10.1128/JCM.01291-14

The incidence of pediatric invasive Haemophilus influenzae and pneumococcal disease in Chiba prefecture, Japan before and after the introduction of conjugate vaccines Reviewed

Naruhiko Ishiwada, Haruka Hishiki, Koo Nagasawa, Sachiko Naito, Yasunori Sato, Bin Chang, Yuko Sasaki, Kouji Kimura, Makoto Ohnishi, Keigo Shibayama

VACCINE Vol. 32 ( 42 ) page： 5425 - 5431 2014.9

Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER SCI LTD

The Haemophilus influenzae type b (Hib) vaccine and the heptavalent pneumococcal conjugate vaccine (PCV7) were introduced in Japan in 2008 and 2010, respectively. In 2011, immunization with these two vaccines was encouraged throughout Japan through a governmental program. Children treated in Chiba prefecture for culture-proven invasive H. influenzae disease (IHiD) and invasive Streptococcus pneumoniae disease (IPD) were identified in a prefectural surveillance study from 2008 to 2013. The incidence rate ratio (IRR) and its confidence interval (CI) were calculated to compare the 3 years before and after governmental financial support for vaccination. The average number of IHiD and IPD cases among children <5 years of age in 2011-2013 decreased 84% (IRR: 0.16,95% CI: 0.09-0.26, p < 0.0001) and 51% (IRR: 0.49,95% CI: 0.37-0.63, p < 0.0001) compared with those occurring in 2008-2010. The most common non-PCV7 serotype encountered in 2011 and 2013 was 19A. After governmental subsidization of Hib and PCV7 vaccination, IHiD and IPD decreased in Chiba prefecture, Japan. Continuous surveillance is necessary to determine the effectiveness of these two vaccines and for detection of emerging invasive serotypes. (C) 2014 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.vaccine.2014.07.100

Practical Agar-Based Disk Potentiation Test for Detection of Fosfomycin-Nonsusceptible Escherichia coli Clinical Isolates Producing Glutathione S-Transferases Reviewed

Genki Nakamura, Jun-ichi Wachino, Natsumi Sato, Kouji Kimura, Keiko Yamada, Wanchun Jin, Keigo Shibayama, Tetsuya Yagi, Kumiko Kawamura, Yoshichika Arakawa

JOURNAL OF CLINICAL MICROBIOLOGY Vol. 52 ( 9 ) page： 3175 - 3179 2014.9

Language：English Publishing type：Research paper (scientific journal) Publisher：AMER SOC MICROBIOLOGY

The number of reports concerning Escherichia coli clinical isolates that produce glutathione S-transferases responsible for fosfomycin resistance (FR-GSTs) has been increasing. We have developed a disk-based potentiation test in which FR-GST producers expand the growth inhibition zone around a Kirby-Bauer disk containing fosfomycin in combination with sodium phosphonoformate (PPF). PPF, an analog of fosfomycin, is a transition-state inhibitor of FosA(PA), a type of FR-GST from Pseudomonas aeruginosa. Considering its mechanism of action, PPF was expected to inhibit a variety of FR-GSTs. In the presence of PPF, zone enlargement around the disk containing fosfomycin was observed for FosA3-, FosA4-, and FosC2-producing E. coli clinical isolates. Moreover, the growth inhibition zone was remarkably enlarged when the Mueller-Hinton (MH) agar plate contained 25 mu g/ml glucose-6-phosphate (G6P). When we retrospectively tested 12 fosfomycin-resistant (MIC, >= 256 mu g/ml) E. coli clinical isolates from our hospital with the potentiation test, 6 FR-GST producers were positive phenotypically by potentiation disk and were positive for FR-GST genes: 5 harbored fosA3 and 1 harbored fosA4. To identify the production of FR-GSTs, we set the provisional cutoff value, 5-mm enlargement, by adding PPF to a fosfomycin disk on the MH agar plates containing G6P. Our disk-based potentiation test reliably identifies FR-GST producers and can be performed easily; therefore, it will be advantageous in epidemiological surveys and infection control of fosfomycin-resistant bacteria in clinical settings.

DOI： 10.1128/JCM.01094-14

Molecular characterization of nicotinate phosphoribosyltransferase from Mycobacterium tuberculosis H37Rv, and inhibition of its activity by pyrazinoic acid

S. Mori, H. Kim, E. Rimbara, Y. Arakawa, K. Shibayama

FEBS JOURNAL Vol. 281 page： 584 - 585 2014.9

Emergence of third-generation cephalosporin-resistant Enterobacteriacae in a Japanese critical care setting. Reviewed International journal

Hagiya H, Murase T, Suzuki M, Otsuka F, Shibayama K

Acute Medicine & Surgery Vol. 1 ( 4 ) page： 256 - 258 2014.7

Language：English Publishing type：Research paper (scientific journal)

Aim: Extended-spectrum beta-lactamases and AmpC beta-lactamases give resistance to Enterobacteriaceae against cephalosporins, which are important drugs in various clinical settings. Within 5 days, a total of eight strains of Citrobacter freundii and two strains of Escherichia coli, all of which were resistant to third-generation cephalosporins, were isolated from six different patients in a critical care center. We carried out epidemiological and genetic studies to confirm the situation to be an outbreak by a single strain or a beta-lactamase-producing gene. Methods: A review of patients' clinical data, pulsed-field gel electrophoresis epidemiological study against the strains, and polymerase chain reaction analysis for the detection of extended-spectrum beta-lactamase genes (TEM, SHV, CTX-M-1, CTX-M-2, and CTX-M-9) and multiplex polymerase chain reaction analysis for plasmid-mediated AmpC-beta-lactamase genes were carried out. Results: Pulsed-field gel electrophoresis showed none of the subjects were genetically identical organisms. However, all strains possessed either extended-spectrum beta-lactamases (TEM, CTX-M-2, and CTX-M-9) or AmpC-beta-lactamases. One patient carried both extended-spectrum beta-lactamase- and AmpC- beta-lactamase-producing organisms. Conclusion: This study revealed that various types of easily transmittable drug resistance genes exist in the critical care setting concurrently. This case was not an outbreak of such strains, but the present situation might indicate a more serious condition than a mere outbreak of a single resistant strain. Enterobacteriaceae that possess extended-spectrum beta-lactamases or AmpC-beta-lactamases are increasingly common in many medical institutions in Japan, and constitutive monitoring of drug-resistant organisms and proper contact precautions are essential to prevent possible outbreaks.

DOI： 10.1002/ams2.64

Food-borne botulism in Japan in March 2012 Reviewed

Yoshika Momose, Hiroshi Asakura, Masaru Kitamura, Yumiko Okada, Yutaka Ueda, Yutaro Hanabara, Tomohiro Sakamoto, Tsuyoshi Matsumura, Masaaki Iwaki, Haru Kato, Keigo Shibayama, Shizunobu Igimi

INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES Vol. 24 page： 20 - 22 2014.7

Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER SCI LTD

In March 2012, two patients were transported urgently to the hospital in Tottori Prefecture, Japan, because of symptoms suggestive of botulism. Botulinum neurotoxin type A was detected in the clinical specimens and the food consumed by the two patients (vacuum packed adzuki-batto, a sweet adzuki bean soup containing noodles). We were able to make a prompt diagnosis of food botulism associated with the consumption of adzuki-batto, from which the causative pathogen Clostridium botulinum Ab was cultured. (C) 2014 The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.

DOI： 10.1016/j.ijid.2014.01.014

Characterization of Multidrug-Resistant Group B Streptococci with Reduced Penicillin Susceptibility Forming Small Non-Beta-Hemolytic Colonies on Sheep Blood Agar Plates Reviewed

Hirotsugu Banno, Kouji Kimura, Yosuke Tanaka, Hiromitsu Kitanaka, Wanchun Jin, Jun-ichi Wachino, Keiko Yamada, Keigo Shibayama, Yoshichika Arakawa

JOURNAL OF CLINICAL MICROBIOLOGY Vol. 52 ( 6 ) page： 2169 - 2171 2014.6

Language：English Publishing type：Research paper (scientific journal) Publisher：AMER SOC MICROBIOLOGY

We isolated and characterized three multidrug-resistant clinical isolates of group B streptococci with reduced penicillin susceptibility (PRGBS) that formed small non-beta-hemolytic colonies on sheep blood agar plates but grew well on chocolate agar plates. They can be overlooked in the bacterial identification step, leading to clinical misdiagnosis and treatment failure.

DOI： 10.1128/JCM.00226-14

Distribution of carbapenem resistance determinants among epidemic and non-epidemic types of Acinetobacter species in Japan Reviewed

Mari Matsui, Satowa Suzuki, Kunikazu Yamane, Masato Suzuki, Toshifumi Konda, Yoshichika Arakawa, Keigo Shibayama

JOURNAL OF MEDICAL MICROBIOLOGY Vol. 63 ( Pt 6 ) page： 870 - 877 2014.6

Language：English Publishing type：Research paper (scientific journal) Publisher：SOC GENERAL MICROBIOLOGY

We performed a comparative molecular analysis on three types of clinically isolated Acinetobacter spp.: epidemic sequence types (STs) of Acinetobacter baumannii (epidemic ST-AB), non-epidemic sequence types of A. baumannii (non-epidemic ST-AB) and non-baumannii Acinetobacter spp. A total of 87 isolates - 46 A. baumannii, 25 A. pittii and 16 A. nosocomialis - from 43 hospitals were analysed. Of these, 31 A. baumannii isolates were ST1 or ST2 according to the Pasteur Institute multilocus sequence typing scheme and were defined as epidemic ST-AB. The other 15 A. baumannii isolates were defined as non-epidemic ST-AB. The epidemic ST-AB isolates harboured the bla(OXA-23-like) gene or had an ISAba1 element upstream of bla(OXA-51-like), or both, whereas non-epidemic ST-AB and non-baumannii Acinetobacter spp. isolates harboured bla(OXA-58-like) or metallo-beta-lactamase genes, or both. The proportion of multidrug-resistant isolates was significantly higher in the epidemic ST-AB isolates (48%) than that in the other types of Acinetobacter isolates (5%) (P<0.05). In addition, epidemic ST-AB isolates exhibited a relatively higher proportion of fluoroquinolone resistance. We demonstrated that, in terms of genotypes and phenotypes of antimicrobial resistance, non-epidemic ST-AB isolates shared more similarity with non-baumannii Acinetobacter spp. isolates than with epidemic ST-AB isolates, regardless of bacterial species. In addition, this study revealed that, even in Japan, where IMP-type metallo-beta-lactamase producers are endemic, epidemic ST-AB harbouring bla(IMP) have not yet emerged.

DOI： 10.1099/jmm.0.069138-0

A subclass B3 metallo--lactamase found in Pseudomonas alcaligenes Reviewed

Masato Suzuki, Satowa Suzuki, Mari Matsui, Yoichi Hiraki, Fumio Kawano, Keigo Shibayama

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY Vol. 69 ( 5 ) page： 1430 - 1432 2014.5

Chromobacterium violaceum nosocomial pneumonia in two Japanese patients at an intensive care unit Reviewed

Hideharu Hagiya, Tomoko Murase, Masato Suzuki, Keigo Shibayama, Yumi Kokumai, Naoto Watanabe, Miyako Maki, Fumio Otsuka

JOURNAL OF INFECTION AND CHEMOTHERAPY Vol. 20 ( 1-2 ) page： 139 - 142 2014.1

Language：English Publishing type：Research paper (scientific journal) Publisher：SPRINGER JAPAN KK

Chromobacterium violaceum is sensitive to temperature and the infection is usually confined to tropical or subtropical regions. Since Japan has a warm climate, C. violaceum has been scarcely isolated from clinical specimens. With global warming, however, the geographical distribution of C violaceum infection is likely to change. We report two cases of C violaceum nosocomial pneumonia that occurred at an intensive care center in Japan. C violaceum was first detected from a patient in the same center as a pathogenic organism of pneumonia. Later, the organism was isolated from sputum and a ventilator circuit tube of another patient in the center. The two patients were admitted to the center in nearby beds for several days. All of the pathogens were confirmed to be C violaceum by the nucleic acid sequence of the 16S rRNA gene and were proven to be genetically identical organisms by pulsed field gel electrophoresis. Both patients were managed with well-humidified and heated oxygen using a venturi mask and ventilator to promote excretion of sputum. It was thought that the medical respiratory care devices that provide a humid and warm environment, an optimal condition for proliferation of C violaceum, can contribute to C violaceum infection in a hospital environment. (C) 2013, Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.jiac.2013.10.001

Evaluation of a Double-Disk Synergy Test with a Common Metallo-beta-Lactamase Inhibitor, Mercaptoacetate, for Detecting NDM-1-Producing Enterobacteriaceae and Acinetobacter baumannii Reviewed

Wachino Jun-ichi, Matsui Mari, Hoang Huy Tran, Suzuki Masato, Suzuki Satowa, Shibayama Keigo

JAPANESE JOURNAL OF INFECTIOUS DISEASES Vol. 67 ( 1 ) page： 66 - 68 2014.1

　 PREV - NEXT 　

1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16