研究者詳細 - 齋藤　竜太

論文 - 齋藤　竜太

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INTRA-OPERATIVE GENETIC ANALYSES FOR DECISION-MAKING DURING TUMOR REMOVAL OF ADULT-TYPE DIFFUSE GLIOMA USING RAPID QUANTITATIVE PCR DEVICE

Ohka, F; Maeda, S; Shimizu, H; Motomura, K; Takeuchi, K; Nagata, Y; Deguchi, S; Shiba, Y; Yamaguchi, J; Takido, Y; Nakamura, A; Ito, S; Saito, R

NEURO-ONCOLOGY 26 巻 2024年11月

ESTABLISHMENT OF PITUITARY NEUROENDOCRINE TUMOR (PITNET) ORGANOID MODELS

Maeda, S; Ohka, F; Kanamori, F; Okumura, Y; Takeuchi, K; Nagata, Y; Yamaguchi, J; Harada, H; Shimizu, H; Takido, Y; Saito, R

NEURO-ONCOLOGY 26 巻 2024年11月

ESTABLISHMENT OF AN APPROPRIATE GLIOMA SURGERY SYSTEM CONSIDERING MOLECULAR AND FUNCTIONAL BOUNDARIES

Saito, R; Motomura, K; Ohka, F; Deguchi, S; Shiba, Y; Yamaguchi, J; Nishikawa, T; Kibe, Y; Shimizu, H; Maeda, S

NEURO-ONCOLOGY 26 巻 2024年11月

DISTANT PARENCHYMAL RECURRENCE DURING LONG-TERM USE OF TTFIELDS TREATMENT FOR GLIOBLASTOMA

Takido, Y; Ohka, F; Deguchi, S; Motomura, K; Mitsuya, K; Shiba, Y; Takeuchi, K; Nagata, Y; Yamaguchi, J; Kibe, Y; Fuse, Y; Maeda, S; Shimizu, H; Nohira, S; Yamamoto, R; Ito, S; Nakamura, A; Saito, R

NEURO-ONCOLOGY 26 巻 2024年11月

Comparison of the inter-laminar approach and laminotomy open approach for filum terminale lipoma: A retrospective analysis 査読有り国際誌

Nawashiro, T; Kurimoto, M; Nagakura, M; Kato, M; Aoki, K; Saito, R

CHILDS NERVOUS SYSTEM 40 巻 ( 11 ) 頁： 3801 - 3810 2024年11月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Child's Nervous System

Purpose: Filum terminale lipoma (FTL) causes spinal-cord tethering and is associated with tethered-cord syndrome, which is treated by dissection of the entrapment. The conventional treatment for FTL involves dissection of the spinal cord through a laminotomy open approach (LOA). However, in recent years, the interlaminar approach (ILA) has gained popularity as a minimally invasive surgery. This study compares the effectiveness of the minimally invasive ILA with the conventional LOA in treating FTL. Methods: We retrospectively evaluated data on the ILA and LOA for FTL at our center. In total, 103 participants were enrolled, including 55 in the ILA group and 48 in the LOA group. Results: The ILA required significantly less surgical time and resulted in less blood loss. The improvement rate of symptoms in symptomatic patients was 84%, and for urinary symptoms and abnormal urodynamic study findings, it was 77%. The postoperative maintenance rate for asymptomatic patients was 100%. Postoperative complications of ILA included delayed wound healing in two patients (3.6%). Conclusion: Compared with LOA, ILA offers advantages in terms of shorter operative time and less blood loss, with no significant difference in long-term symptom-improvement rates between the groups.

DOI： 10.1007/s00381-024-06507-4

Three-dimensional spiral-shaping method of microcatheter for paraclinoid aneurysms: assessment using silicone models

Imaoka, E; Nishihori, M; Izumi, T; Goto, S; Araki, Y; Yokoyama, K; Uda, K; Kanamori, F; Saito, R

NAGOYA JOURNAL OF MEDICAL SCIENCE 86 巻 ( 4 ) 頁： 655 - 664 2024年11月

記述言語：英語出版者・発行元：Nagoya Journal of Medical Science

Selecting an appropriate microcatheter tip shape for paraclinoid aneurysms is difficult. Therefore, we devised an original simple and uniform three-dimensional (3D) spiral-shaping method of microcatheter and validated the characteristics and usefulness of this method for coil embolization of paraclinoid aneurysms using patient-specific silicone models. These silicone models were produced based on clinical data from four patients with four paraclinoid aneurysms that underwent endovascular treatment using the 3D spiral-shaping method. These models were classified into four types: superior, medial, inferior, and lateral corresponding to the aneurysm protrusion and locations (C3 or C2 segments by Fisher’s classification). Employing a pulsatile pump setup, two operators assessed the following items: navigation methods (pull and wire guiding), catheterization times, microcatheter tip position in the aneurysm, and the feasibility of inserting a framing coil by simple technique compared with three other shapes (straight, 90, pigtail). Three-dimensional spiral-shaped microcatheter could be placed in the medial and inferior type models of C3 segments and superior type model of C2 segment by the pullback method. Catheterization times using a 3D spiral-shaped catheter were significantly shorter than other shaped ones in the superior type models. No significant difference was found in another silicone model. Three-dimensional spiral- and pigtail-shaped catheters tended to position the tip at the center of the aneurysm. In conclusion, 3D spiral-shaped microcatheter was especially effective for the superior projected aneurysm at the C2 segment. The 3D spiral-shaping method can provide easy and secure navigation of the microcatheter into the paraclinoid aneurysms, ensuring optimal positioning for coil insertion.

DOI： 10.18999/nagjms.86.4.655

Web of Science

Scopus

PubMed

Efficacy and safety of endovascular coil embolization for unruptured middle cerebral artery aneurysms: middle-term clinical and imaging outcomes with 3 years mean follow-up periods, a 16-year experience

Hanyu, T; Izumi, T; Tanei, T; Nishihori, M; Goto, S; Araki, Y; Yokoyama, K; Miyachi, S; Saito, R

NAGOYA JOURNAL OF MEDICAL SCIENCE 86 巻 ( 4 ) 頁： 596 - 607 2024年11月

記述言語：英語出版者・発行元：Nagoya Journal of Medical Science

The anatomical characteristics of middle cerebral artery aneurysms make endovascular treatment difficult. This study evaluated the efficacy and safety of endovascular treatment of unruptured middle cerebral artery aneurysm in preventing rupture. A retrospective review of patients who underwent coil embolization for unruptured middle cerebral artery aneurysm between 2006 and 2022 at Nagoya University Hospital with at least 12 months followed up was conducted. Imaging and clinical outcomes were described using the Raymond classification and the modified Rankin Scale, respectively. Good imaging outcome was defined as complete occlusion or neck remnant and clinical outcome as modified Rankin Scale score of 0–2. Patients were divided into initial and recurrent group based on the number of treatments, pre- and post-stent groups based on when stents became available in Japan. A total of 77 patients (80 with aneurysms) were included in the final analysis. Their average age was 60.3 years, and their average follow-up period was 38 months. Favorable clinical outcomes were achieved for 96.2% among 66 (97.0%) initial and 11 (91.7%) recurrent aneurysms. Furthermore, good imaging outcomes were obtained in 90.0 %, and 5% had permanent symptomatic ischemic complications. The pre-stent group had a significantly higher proportion of patients with narrow-neck aneurysms than the post-stent group. There were no significant differences in terms of imaging and clinical outcomes or complication rates. The present study demonstrated that endovascular treatment of unruptured middle cerebral artery aneurysm was safe and effective in preventing rupture. The wide-neck aneurysm was also well embolized by using adjunctive technique.

DOI： 10.18999/nagjms.86.4.596

Web of Science

Scopus

PubMed

Dual phenotypes in recurrent astrocytoma, IDH-mutant; coexistence of IDH-mutant and IDH-wildtype components: a case report with genetic and epigenetic analysis 国際誌 Open Access

Yamaguchi, J; Ohka, F; Seki, M; Motomura, K; Deguchi, S; Shiba, Y; Okumura, Y; Kibe, Y; Shimizu, H; Maeda, S; Takido, Y; Yamamoto, R; Nakamura, A; Karube, K; Saito, R

ACTA NEUROPATHOLOGICA COMMUNICATIONS 12 巻 ( 1 ) 頁： 169 - 169 2024年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Acta Neuropathologica Communications

Mutations in the isocitrate dehydrogenase (IDH) gene are recognized as the key drivers in the oncogenesis of astrocytoma and oligodendroglioma. However, the significance of IDH mutation in tumor maintenance and malignant transformation has not been elucidated. We encountered a unique case of IDH-mutant astrocytoma that, upon malignant transformation, presented two distinct intratumoral components: one IDH-wildtype and one IDH-mutant. The IDH-wild-type component exhibited histological findings similar to those of small cell-type glioblastoma with a higher Ki-67 index than the IDH-mutant component. Despite their genetic divergence, both components exhibited similar comprehensive methylation profiles within the CpG island and were classified into methylation class of “Astrocytoma, IDH-mutant; High Grade” by the German Cancer Center (DKFZ) classifier v11.4. Phylogenetic analysis demonstrated that the IDH-wildtype component emerged as a subclonal component of the primary tumor. Detailed molecular analyses revealed that the loss of the IDH mutation was induced by the hemizygous loss of the entire arm of chromosome 2, on which IDH1 gene is located. Notably, the IDH-wild-type subclones uniquely acquired CDKN2A/B homozygous deletion and PDGFRA amplification, which is a marker of the aggressive phenotype of astrocytoma, IDH-mutant. Because these genetic abnormalities can drive oncogenic pathways, such as the PI3K/AKT/mTOR and RB signaling pathway, IDH-mutant gliomas that acquired these mutations were no longer dependent on the initial driver mutation, the IDH mutation. Molecular analysis of this unique case provides insight that in a subset of astrocytoma, IDH-mutant that acquired these genetic abnormalities, IDH mutation may not play a pivotal role in tumor growth and acquisition of these genetic abnormalities may contribute to the acquisition of resistance to IDH inhibitors.

DOI： 10.1186/s40478-024-01879-9

Recurrent symptomatic intracranial hemorrhage in high-grade astrocytoma with piloid features: illustrative case 査読有り Open Access

Niwa, H; Kato, T; Hasegawa, T; Kuwabara, K; Ohka, F; Hirato, J; Sasaki, S; Ichimura, K; Yoshioka, T; Naito, T; Mizuno, A; Kageyama, A; Oishi, H; Saito, R

JOURNAL OF NEUROSURGERY-CASE LESSONS 8 巻 ( 15 ) 2024年10月

記述言語：英語出版者・発行元：Journal of Neurosurgery: Case Lessons

BACKGROUND High-grade astrocytoma with piloid features (HGAP) is a novel condition introduced in the 2021 World Health Organization classification. Given that it has been recently classified, reports clarifying its clinical features or diagnostic criteria are lacking, especially in cases of atypical presentation. Herein, the authors present a rare case of HGAP with repeated symptomatic hemorrhages. OBSERVATIONS A woman in her 20s presented with an acute headache and vertigo. Computed tomography and magnetic resonance imaging revealed a 2.5 × 2.8 × 2.3–cm hemorrhagic cerebellar mass with calcifications. After moderate improvement of her symptoms, she developed recurrent hemorrhage, and the tumor size increased (3.0 × 3.6 × 4.0 cm) 18 days later, necessitating resection. Pathological and molecular analyses confirmed the diagnosis of HGAP with an FGFR1-TACC1 fusion, MTAP/CDKN2A/B deletion, and SETD2 rearrangement. Radiologically, the presence of calcification and cystic components and the absence of perilesional edema were atypical features of previously reported HGAP. LESSONS Although recurrent symptomatic intracranial hemorrhages are rare in HGAP, enhancing lesions on magnetic resonance imaging suggest the need for resection to obtain tissue for molecular diagnosis and guide adjuvant treatment strategies.

DOI： 10.3171/CASE24395

Exo- and endoscopic two-step approach for meningeal tumours invading the lateral wall of large dural venous sinuses: how I do it 査読有り国際誌 Open Access

Iwami, K; Watanabe, T; Takeuchi, K; Saito, R

ACTA NEUROCHIRURGICA 166 巻 ( 1 ) 頁： 397 - 397 2024年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Acta Neurochirurgica

Background: Treating meningeal tumours invading the large dural venous sinuses is a subject of debate regarding the approach for removing the intra-sinus components. Additionally, directly observing the invasion site of tumours invading the lateral wall of the sinus is difficult. Method: We describe our exo- and endoscopic two-step approach (EETA): an exoscope is used to remove the extra-sinus component, while an endoscope is used to observe the invaded lateral wall and remove the intra-sinus component. Conclusion: EETA can be a viable option for treating meningeal tumours invading the venous sinus owing to its high resection rate and low invasiveness.

DOI： 10.1007/s00701-024-06298-2

Four-dimensional digital subtraction angiography for the vascular anatomical diagnosis of dural arteriovenous malformation: Comparison with the conventional method 国際誌 Open Access

Ishikawa, K; Nishihori, M; Izumi, T; Oshima, R; Uemura, T; Kanamori, F; Uda, K; Yokoyama, K; Araki, Y; Saito, R

INTERVENTIONAL NEURORADIOLOGY 30 巻 ( 5 ) 頁： 738 - 745 2024年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Interventional Neuroradiology

Background: Two-dimensional digital subtraction angiography (2D-DSA) and conventional three-dimensional digital subtraction angiography (3D-DSA) are used for the detailed analysis of dural arteriovenous fistula (DAVF). Recently, four-dimensional digital subtraction angiography (4D-DSA), a novel technology, has been attracting attention. The current study aimed to evaluate the capability of 4D-DSA in assessing anatomical angioarchitecture in DAVF. Methods: In total, 10 consecutive patients with DAVF who underwent 3D-DSA and 4D-DSA at a single institution were included in the analysis. Initially, one-slice multiplanar reconstruction (MPR) images obtained via 4D-DSA and 3D-DSA were compared to investigate the visibility of the feeding artery, fistulous point, and draining vein. Next, 4D-DSA images alone were compared and evaluated with and the MPR images of conventional 3D-DSA in terms of diagnosis of the angioarchitecture. Results: In total, six men and four women (with a mean age of 65.6 ± 10.0 years) were included in the study. The MPR image obtained via 3D-DSA had a significantly better visibility of the feeding artery and fistulous point than that acquired via 4D-DSA (p < 0.05). As for the draining vein, the score was equivalent and not significant. The diagnosis of the vascular architecture of only 4D-DSA images was nearly equivalent to that of MPR images of 3D-DSA. There were no inter-rater differences. Conclusion: The MPR images obtained via 4D-DSA may be slightly inferior to those acquired via 3D-DSA in identifying fine angioarchitecture in DAVF. However, they were comparable in terms of diagnostic accuracy.

DOI： 10.1177/15910199221145526

Novel case of ependymoma-like tumor with mesenchymal differentiation harboring <i>ZFTA</i>::<i>RELA</i> fusion in an adult 査読有り

Yajima, H; Takayanagi, S; Takami, H; Tanaka, S; Nomura, M; Satomi, K; Ikemura, M; Nobusawa, S; Saito, R; Kondo, A; Saito, N

BRAIN TUMOR PATHOLOGY 41 巻 ( 3-4 ) 頁： 139 - 144 2024年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Brain Tumor Pathology

High-grade supratentorial tumors harboring ZFTA::NCOA1/2 fusion in infants presenting with mixed histology of embryonal-appearing components resembling ependymoma and mesenchymal sarcomatous components have recently been reported as ependymoma-like tumors with mesenchymal differentiation (ELTMDs). In contrast, we describe herein a pathologically similar case with a novel ZFTA::RELA fusion in an adult. A frontal lobe lesion was resected from a 30-year-old woman and displayed mixed components on pathological examination, showing ependymoma-like and sarcomatous parts. The absence of perivascular pseudorosettes was inconsistent with a diagnosis of ependymoma. Fluorescence in situ hybridization analysis confirmed ZFTA::RELA fusion. The DKFZ methylation classifier (v12.8) did not categorize this case among established methylation classes. In addition, t-distributed stochastic neighbor embedding analysis using DNA methylation data revealed that the present case was distant from ependymomas but close to two previously reported cases of ELTMD involving ZFTA::NCOA1/2 fusion. Taken together, we concluded that this tumor should be considered under the entity of ELTMD. This represents the first description of an adult patient with ELTMD harboring ZFTA::RELA fusion analyzed by DNA methylation profiling, supporting the establishment of ELTMD as a possible new tumor type.

DOI： 10.1007/s10014-024-00489-6

Clinical significance of cerebral microbleeds in patients with germinoma who underwent long-term follow-up 査読有り国際誌 Open Access

Kanamori, M; Mugikura, S; Iizuka, O; Mori, N; Shimoda, Y; Shibahara, I; Umezawa, R; Jingu, K; Saito, R; Sonoda, Y; Kumabe, T; Suzuki, K; Endo, H

JOURNAL OF NEURO-ONCOLOGY 170 巻 ( 1 ) 頁： 173 - 184 2024年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of Neuro-Oncology

Purpose: This study identified the factors affecting cerebral microbleed (CMBs) development. Moreover, their effects on intelligence and memory and association with stroke in patients with germinoma who had long-term follow-up were evaluated. Methods: This study included 64 patients with germinoma who were histologically and clinically diagnosed with and treated for germinoma. These patients were evaluated cross-sectionally, with a focus on CMBs on susceptibility-weighted magnetic resonance imaging (SWI), brain atrophy assessed through volumetric analysis, and intelligence and memory. Results: The follow-up period was from 32 to 412 (median: 175.5) months. In total, 43 (67%) patients had 509 CMBs and 21 did not have CMBs. Moderate correlations were observed between the number of CMBs and time from initial treatments and recurrence was found to be a risk factor for CMB development. Increased temporal CMBs had a marginal effect on the processing speed and visual memory, whereas brain atrophy had a statistically significant effect on verbal, visual, and general memory and a marginal effect on processing speed. Before SWI acquisition and during the follow-up periods, eight strokes occurred in four patients. All of these patients had ≥ 15 CMBs on SWI before stroke onset. Meanwhile, 33 patients with < 14 CMBs or 21 patients without CMBs did not experience stroke. Conclusion: Patients with a longer time from treatment initiation had a higher number of CMBs, and recurrence was a significant risk factor for CMB development. Furthermore, brain atrophy had a stronger effect on memory than CMBs. Increased CMBs predict the stroke onset.

DOI： 10.1007/s11060-024-04753-9

Epileptic foci and networks in children with epilepsy after acute encephalopathy with biphasic seizures and late reduced diffusion 査読有り国際誌

Mitsumatsu, T; Ito, Y; Maki, Y; Yamamoto, H; Sawamura, F; Ishizaki, T; Maesawa, S; Bagarinao, E; Nakata, T; Kidokoro, H; Saito, R; Natsume, J

BRAIN & DEVELOPMENT 46 巻 ( 9 ) 頁： 302 - 307 2024年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Brain and Development

Background: Acute encephalopathy with biphasic seizures and late reduced diffusion (AESD) develops along with status epilepticus and widespread subcortical white matter edema. We aimed to evaluate the epileptic foci and networks in two patients with epilepsy after AESD using simultaneous electroencephalography and functional magnetic resonance imaging (EEG-fMRI). Methods: Statistically significant blood oxygen level-dependent (BOLD) responses related to interictal epileptiform discharges (IEDs) were analyzed using an event-related design of hemodynamic response functions with multiple peaks. Results: Patient 1 developed focal seizures at age 10 years, one year after AESD onset. Positive BOLD changes were observed in the bilateral frontotemporal lobes, left parietal lobe, and left insula. BOLD changes were also observed in the subcortical structures. Patient 2 developed epileptic spasms at age two years, one month after AESD onset. Following total corpus callosotomy (CC) at age three years, the epileptic spasms resolved, and neurodevelopmental improvement was observed. Before CC, positive BOLD changes were observed bilaterally in the frontotemporal lobes. BOLD changes were also observed in the subcortical structures. After CC, the positive BOLD changes were localized in the temporal lobe ipsilateral to the IEDs, and the negative BOLD changes were mainly in the cortex and subcortical structures of the hemisphere ipsilateral to IEDs. Conclusion: EEG-fMRI revealed multiple epileptic foci and extensive epileptic networks, including subcortical structures in two cases with post-AESD epilepsy. CC may be effective in disconnecting the bilaterally synchronous epileptic networks of epileptic spasms after AESD, and pre-and post-operative changes in EEG-fMRI may reflect improvements in epileptic symptoms.

DOI： 10.1016/j.braindev.2024.07.003

Fact-finding survey of gynecologic oncologist on appearance care for cancer patients in Japan

Kuji, S; Nozawa, K; Toma, S; Arao, H; Imoto, S; Kajiyama, H; Kitano, A; Kodera, Y; Saito, R; Sakai, R; Taketomi, A; Nakayama, R; Hiyama, E; Futamura, M; Matsui, M; Yonemura, M; Oya, M; Suzuki, N

INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER 34 巻 ( SUPPL_3 ) 頁： A328 - A328 2024年10月

がん診療ガイドライン作成・改訂に関する問題点と対応について脳腫瘍診療ガイドラインの現状と課題

園田順彦, 中田光俊, 田中將太, 篠山隆司, 吉本幸司, 成田善孝, 荒川芳輝, 山崎文之, 齋藤竜太, 阿部竜也, 黒住和彦, 市川智継, 永根基雄

日本癌治療学会学術集会抄録集 62回巻頁： GL - 4 2024年10月

下垂体腫瘍に対する治療

竹内和人, 永田雄一, 齋藤竜太

日本内分泌学会雑誌 100 巻 ( 2 ) 頁： 565 - 565 2024年10月

Cost of medical care for malignant brain tumors at hospitals in the Japan Clinical Oncology Group brain-tumor study group 査読有り Open Access

Motomura, K; Sasaki, K; Sugii, N; Yamaguchi, S; Inoue, H; Oshima, A; Tanaka, K; Otani, Y; Shirahata, M; Shibahara, I; Nagane, M; Tsuzuki, S; Matsutani, T; Tsukamoto, Y; Kijima, N; Asano, K; Ohno, M; Inoue, A; Mineharu, Y; Miyake, K; Mitobe, Y; Hanihara, M; Kawanishi, Y; Deguchi, S; Saito, M; Matsuda, R; Ujifuku, K; Arita, H; Sato, Y; Yamashita, S; Yonezawa, U; Yamaguchi, J; Momii, Y; Ogawa, T; Kambe, A; Ohba, S; Fukai, J; Saito, N; Kinoshita, M; Sumi, K; Otani, R; Uzuka, T; Takebe, N; Koizumi, S; Saito, R; Arakawa, Y; Narita, Y

JAPANESE JOURNAL OF CLINICAL ONCOLOGY 54 巻 ( 10 ) 頁： 1123 - 1131 2024年9月

記述言語：英語出版者・発行元：Japanese Journal of Clinical Oncology

Background: This study aimed to investigate what treatment are selected for malignant brain tumors, particularly glioblastoma (GBM) and primary central nervous system lymphoma (PCNSL), in real-world Japan and the costs involved. Methods: We conducted a questionnaire survey regarding treatment selections for newly diagnosed GBM and PCNSL treated between July 2021 and June 2022 among 47 institutions in the Japan Clinical Oncology Group-Brain Tumor Study Group. We calculated the total cost and cost per month of the initial therapy for newly diagnosed GBM or PCNSL. Results: The most used regimen (46.8%) for GBM in patients aged ≤74 years was 'Surgery + radiotherapy concomitant with temozolomide'. This regimen's total cost was 7.50 million JPY (Japanese yen). Adding carmustine wafer implantation (used in 15.0%), TTFields (used in 14.1%), and bevacizumab (BEV) (used in 14.5%) to the standard treatment of GBM increased the cost by 1.24 million JPY for initial treatment, and 1.44 and 0.22 million JPY per month, respectively. Regarding PCNSL, 'Surgery (biopsy) + rituximab, methotrexate, procarbazine, and vincristine (R-MPV) therapy' was the most used regimen (42.5%) for patients of all ages. This regimen incurred 1.07 million JPY per month. The three PCNSL regimens based on R-MPV therapy were in ultra-high-cost medical care (exceeding 1 million JPY per month). Conclusions: Treatment of malignant brain tumors is generally expensive, and cost-ineffective treatments such as BEV are frequently used. We believe that the results of this study can be used to design future economic health studies examining the cost-effectiveness of malignant brain tumors.

DOI： 10.1093/jjco/hyae116

Isolation of ependymal cilia from mouse brain 査読有り国際誌 Open Access

Mizuno, A; Takeuchi, K; Nagata, Y; Harada, H; Yamamoto, T; Ishikawa, T; Maeda, S; Ohka, F; Ueno, H; Saito, R

JOURNAL OF NEUROSCIENCE METHODS 409 巻頁： 110198 - 110198 2024年9月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of Neuroscience Methods

Background: Ependymal cilia play a major role in the circulation of cerebrospinal fluid. Although isolation of cilia is an essential technique for investigating ciliary structure, to the best of our knowledge, no report on the isolation and structural analysis of ependymal cilia from mouse brain is available. New method: We developed a novel method for isolating ependymal cilia from mouse brain ventricles. We isolated ependymal cilia by partially opening the lateral ventricles and gently applying shear stress, followed by pipetting and ultracentrifugation. Results: Using this new method, we were able to observe cilia separately. The results demonstrated that our method successfully isolated intact ependymal cilia with preserved morphology and ultrastructure. In this procedure, the ventricular ependymal cell layer was partially detached. Comparison with existing methods: Compared to existing methods for isolating cilia from other tissues, our method is meticulously tailored for extracting ependymal cilia from the mouse brain. Designed with a keen understanding of the fragility of the ventricular ependyma, our method prioritizes minimizing tissue damage during the isolation procedure. Conclusions: We isolated ependymal cilia from mouse brain by applying shear stress selectively to the ventricles. Our method can be used to conduct more detailed studies on the structure of ependymal cilia.

DOI： 10.1016/j.jneumeth.2024.110198

A Combination Technique of N-butyl-2-cyanoacrylate and Minimal Coils with Flow Control for Parent Artery Occlusion of a Giant Internal Carotid Artery Aneurysm: A Technical Note Open Access

Hanyu, T; Izumi, T; Tanei, T; Nishihori, M; Gotoh, S; Araki, Y; Yokoyama, K; Saito, R

NEUROLOGIA MEDICO-CHIRURGICA 64 巻 ( 9 ) 頁： 347 - 351 2024年9月

記述言語：英語出版者・発行元：Neurologia Medico-Chirurgica

Parent artery occlusion for large or giant internal carotid artery aneurysms remains a necessary procedure in the era of flow diverters. Endovascular parent artery occlusion is currently performed using detachable balloons or coils, which are difficult to obtain or costly. At our institution, we have devised a technique for combining n-butyl-2-cyanoacrylate and coils with flow control to solve this problem. Patients who underwent parent artery occlusion for large or giant internal carotid artery aneurysms with a follow-up period of more than 12 months were included. Imaging outcomes were evaluated for complete or incomplete aneurysmal occlusion and with or without aneurysmal shrinkage. The clinical outcome was based on changes in the modified Rankin Scale. Ten patients (ten aneurysms) were in-cluded. Their average age and average follow-up period were 68.4 years and 36 months, respectively. Complete occlusion and favorable clinical outcome were observed in all cases. The parent artery occlusion using a combination of coils and n-butyl-2-cyanoacrylate with flow control technique is effective for both imaging and clinical outcomes.

DOI： 10.2176/jns-nmc.2024-0046

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