Faculty Profiles - ABE Hiroshi

Papers - ABE Hiroshi

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Fluorogenic probes using 4-substituted-2-nitrobenzenesulfonyl derivatives as caging groups for the analysis of human glutathione transferase catalyzed reactions Reviewed

Aya Shibata, Yukiko Nakano, Mika Ito, Mika Araki, Jie Zhang, Yasuhiko Yoshida, Satoshi Shuto, Bengt Mannervik, Ralf Mogenstern, Yoshihiro Ito, Hiroshi Abe

Analyst Vol. 138 ( 24 ) page： 7326 - 7330 2013

Language：English Publishing type：Research paper (scientific journal)

We have synthesized a series of 4-substituted-2-nitrobenzene-sulfonyl compounds for caged fluorogenic probes and conducted a Hammett plot analysis using the steady-state kinetic parameters. The results revealed that the glutathione transferase (GST) alpha catalyzed reaction was dependent on the σ value in the same way as the non-enzymatic reaction, whereas the dependence of the σ value of the GST mu and pi was not as pronounced as that of GST alpha. © 2013 The Royal Society of Chemistry.

DOI： 10.1039/c3an01339a

RNA-templated molecule release induced protein expression in bacterial cells Reviewed

Aya Shibata, Yoshihiro Ito, Hiroshi Abe

CHEMICAL COMMUNICATIONS Vol. 49 ( 3 ) page： 270 - 272 2013

Language：English Publishing type：Research paper (scientific journal) Publisher：ROYAL SOC CHEMISTRY

We have developed a system to release a biologically active molecule in response to the sequence of a target gene. The releasing system, which was triggered by the reduction of an azidomethyl group, was successfully applied to protein expression induced by the release of IPTG triggered by endogenous RNA in bacterial cells.

DOI： 10.1039/c2cc37826d

One-pot ring-closing metathesis/1,3-dipolar cycloaddition through assisted tandem ruthenium catalysis: Synthesis of a dye with isoindolo[2,1-a]quinoline structure Reviewed

Mitsuhiro Arisawa, Yuki Fujii, Hiroshige Kato, Hayato Fukuda, Takashi Matsumoto, Mika Ito, Hiroshi Abe, Yoshihiro Ito, Satoshi Shuto

Angewandte Chemie - International Edition Vol. 52 ( 3 ) page： 1003 - 1007 2013

Language：English Publishing type：Research paper (scientific journal)

The one-pot tandem reaction of N-alkyl-N-allyl-2-vinylaniline derivatives with benzo- or naphthoquinones and a ruthenium-alkylidene catalyst leads to isoindolo[2,1-a]quinolines in a variety of colors, which can be altered by exchanging the substituent on the core heterocycle (see scheme). This reaction offers a new synthetic method for π-conjugated small molecules from simple aniline derivatives. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

DOI： 10.1002/anie.201206765

Cationic cholesterol-modified gelatin as an in vitro siRNA delivery vehicle Reviewed

Pallavi Ananda Kadengodlu, Toshiro Aigaki, Hiroshi Abe, Yoshihiro Ito

Molecular BioSystems Vol. 9 ( 5 ) page： 965 - 968 2013

Language：English Publishing type：Research paper (scientific journal)

Polymeric micelle prepared through the self-assembly of cationic cholesterol-modified gelatin was tested for siRNA delivery. It exerted the desired effect of gene knockdown in HeLa cells stably expressing the luciferase gene and achieved a two-fold increase in the knockdown ability when compared to Lipofectamine® 2000. It was found that the polymeric micelle exhibited excellent stability and increased the biological stability of the siRNA in serum. © 2013 The Royal Society of Chemistry.

DOI： 10.1039/c2mb25424g

Rolling circle amplification in a prokaryotic translation system using small circular RNA Reviewed

Naoko Abe, Michio Hiroshima, Hideto Maruyama, Yuko Nakashima, Yukiko Nakano, Akira Matsuda, Yasushi Sako, Yoshihiro Ito, Hiroshi Abe

Angewandte Chemie - International Edition Vol. 52 ( 27 ) page： 7004 - 7008 2013

Language：English Publishing type：Research paper (scientific journal)

Getting the runaround: Small circular RNA molecules containing an infinite open reading frame were synthesized and tested in an E. coli cell-free translation system. A circular RNA 126 nucleotides in length was found to produce more product than its linear counterpart by two orders of magnitude, because a ribosome can work more effectively towards the elongation on circular RNA than it can on linear RNA in this continuous peptide synthesis. Copyright © 2013 WILEY-VCH Verlag GmbH &amp
Co. KGaA, Weinheim.

DOI： 10.1002/anie.201302044

ナノ構造化RNAを用いたRNA干渉法

阿部洋

薬学雑誌 Vol. 133 ( 3 ) page： 373-378 2013

"PEGylated Antibodies and DNA in Organic Media and Genetic PEGylation" in "Green Polymer 2"

Seiichi Tada, Horoshi Abe, and Yoshihiro Ito

American Chemical Society page： 223-233 2013

PEGylated Antibodies and DNA in Organic Media and Genetic PEGylation

Tada Seiichi, Abe Hiroshi, Ito Yoshihiro

GREEN POLYMER CHEMISTRY: BIOCATALYSIS AND MATERIALS II Vol. 1144 page： 223 - 233 2013

Peroxidase Activity of G-Quadruplex Hemin-Binding DNA Aptamers Determined by Electrochemical Measurement

Kubo I., Eguchi T., Hoshino Y., Liu M., Abe H., Ito T.

BIOENGINEERING BASED ON ELECTROCHEMISTRY Vol. 50 ( 28 ) page： 1 - 7 2013

Publisher：ECS Transactions

Certain guanine-rich DNA oligomers that form a G-quadruplex structure and bind hemin inside are called hemin-binding DNA aptamers. A hemin-binding DNA aptamer with the 4c15 sequence forms a parallel G quadraplex and the PS2.M sequence folds and forms an anti-parallel structure. We propose a novel method to examine the peroxidase activity of hemin-binding DNA aptamers through electrochemical measurements. In this study, a polyA chain and thiol were introduced to the 5' terminal of each oligonucleotide. The peroxidase activity of hemin-binding DNA aptamers was determined after modifying a gold electrode with the aptamers. The distance to hemin from the surface of the electrode is most important to determine catalytic activity. Catalytic activity, shown as KM of the examined parallel DNA aptamers, was around 20 μM. 4c15 without a poly A linker showed the highest catalytic activity, and its KM was 19 μM. The KM of the anti-parallel aptamer was almost the same as that of the parallel aptamer. © The Electrochemical Society.

DOI： 10.1149/05028.0001ecst

Web of Science

Scopus

Immobilization of epidermal growth factor on titanium and stainless steel surfaces via dopamine treatment Reviewed

Jeonghwa Kang, Makoto Sakuragi, Aya Shibata, Hiroshi Abe, Takashi Kitajima, Seiichi Tada, Masayoshi Mizutani, Hitoshi Ohmori, Hirohito Ayame, Tae Il Son, Toshiro Aigaki, Yoshihiro Ito

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS Vol. 32 ( 8 ) page： 2552 - 2561 2012.12

Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER SCIENCE BV

Titanium and stainless steel were modified with dopamine for the immobilization of biomolecules, epidermal growth factor (EGF). First, the treatment of metal surfaces with a dopamine solution under different pH conditions was investigated. At higher pH, the dopamine solution turned brown and formed precipitates. Treatment of the metals with dopamine at pH 8.5 also resulted in the development of brown color at the surface of the metals. The hydrophobicity of the surfaces increased after treatment with dopamine, independently of pH. X-ray photoelectron spectroscopy revealed the formation of a significant amount of an organic layer on both surfaces at pH 8.5. According to ellipsometry measurements, the organic layer formed at pH 8.5 was about 1000 times as thick as that formed at pH 4.5. The amount of amino groups in the layer formed at pH 8.5 was also higher than that observed in the layer formed at pH 4.5. EGF molecules were immobilized onto the dopamine-treated surfaces via a coupling reaction using carbodiimide. A greater amount of EGF was immobilized on surfaces treated at pH 8.5 compared with pH 4.5. Significantly higher growth of rat fibroblast cells was observed on the two EGF-immobilized surfaces compared with non-immobilized surfaces in the presence of EGF. The present study demonstrated that metals can become bioactive via the surface immobilization of a growth factor and that the effect of the immobilized growth factor on metals was greater than that of soluble growth factor. (C) 2012 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.msec.2012.07.039

Web of Science

Scopus

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Detection of pre-mRNA splicing in vitro by an RNA-templated fluorogenic reaction Reviewed

Yasutsugu Tamura, Kazuhiro Furukawa, Rei Yoshimoto, Yuto Kawai, Minoru Yoshida, Satoshi Tsuneda, Yoshihiro Ito, Hiroshi Abe

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS Vol. 22 ( 23 ) page： 7248 - 7251 2012.12

Language：English Publishing type：Research paper (scientific journal) Publisher：PERGAMON-ELSEVIER SCIENCE LTD

RNA splicing is an important target for basic research of disease mechanisms and for drug discovery. Here, we report a new method for analysis of the in vitro RNA splicing process that produces fluorescence using a reduction-triggered fluorescence (RETF) probe. The fluorescence signal is produced only when the two probes bind side-by-side with a specific RNA target. Precursor messenger RNA and mature messenger RNA originating from the chicken delta-crystallin (CDC) gene were successfully discriminated in solution using an RETF probe with the assistance of helper oligonucleotide strands. Also, we successfully applied RETF probes to the detection of emerging mature mRNA in an in vitro splicing process. (C) 2012 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.bmcl.2012.09.033

ナノ構造を制御したRNA干渉法

阿部洋、阿部奈保子、柴田綾、伊藤嘉浩

アンチセンスDNA/RNA研究会誌 Vol. 16 ( 2 ) page： 3 2012.11

Design and synthesis of indomethacin analogues that inhibit P-glycoprotein and/or multidrug resistant protein without Cox inhibitory activity Reviewed

Mitsuhiro Arisawa, Yayoi Kasaya, Tohru Obata, Takuma Sasaki, Tomonori Nakamura, Takuya Araki, Koujirou Yamamoto, Akito Sasaki, Akihito Yamano, Mika Ito, Hiroshi Abe, Yoshihiro Ito, Satoshi Shuto

Journal of Medicinal Chemistry Vol. 55 ( 18 ) page： 8152 - 8163 2012.9

Language：English Publishing type：Research paper (scientific journal)

We designed and synthesized conformationally restricted analogues and regioisomers of the nonsteroidal anti-inflammatory drug indomethacin. Evaluation of the inhibitory effects of these compounds on COX, P-glycoprotein, and multidrug resistance indicated that NSAIDS modulation of multidrug-resistant P-glycoprotein and multidrug-resistant protein-1 is not associated with COX-1 and COX-2 inhibitory activities. © 2012 American Chemical Society.

DOI： 10.1021/jm301084z

Development of new luminogenic probes for detection of glutathione s-transferase activity in living cells

Ito Mika, Shibata Aya, Abe Hiroshi, Zhang Jie, Morgenstern Ralf, Shuto Satoshi, Ito Yoshihiro

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY Vol. 244 2012.8

Development of new luminogenic probes for detection of glutathione s-transferase activity in living cells Reviewed

Mika Ito, Aya Shibata, Hiroshi Abe, Jie Zhang, Ralf Morgenstern, Satoshi Shuto, Yoshihiro Ito

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY Vol. 244 2012.8

Universal Caging Group for the in-Cell Detection of Glutathione Transferase Applied to 19F NMR and Bioluminogenic Probes Reviewed

Mika Ito, Aya Shibata, Jie Zhang, Michio Hiroshima, Yasushi Sako, Yukiko Nakano, Kyoko Kojima-Aikawa, Bengt Mannervik, Satoshi Shuto, Yoshihiro Ito, Ralf Morgenstern, Hiroshi Abe

CHEMBIOCHEM Vol. 13 ( 10 ) page： 1428 - 1432 2012.7

還元反応を引き金とする off/on 型蛍光プローブを用いたRNA検出法の開発

阿部洋, 柴田綾, 古川和寛, 常田聡, 伊藤嘉浩

化学と生物 Vol. 50 ( 7 ) page： 540 - 544 2012.7

環元反応を引き金とするoff/on 型蛍光プローブを用いたRNA検出法の開発

阿部洋、柴田綾、古川和寛、常田聡、伊藤嘉浩

化学と生物 Vol. 50 ( 7月 ) page： 540 2012.7

Oligonucleotide-Templated Reactions for Sensing Nucleic Acids Reviewed

Aya Shibata, Hiroshi Abe, Yoshihiro Ito

MOLECULES Vol. 17 ( 3 ) page： 2446 - 2463 2012.3

Language：English Publishing type：Research paper (scientific journal) Publisher：MDPI AG

Oligonucleotide-templated reactions are useful for applying nucleic acid sensing. Various chemistries for oligonucleotide-templated reaction have been reported so far. Major scientific interests are focused on the development of signal amplification systems and signal generation systems. We introduce the recent advances of oligonucleotide-templated reaction in consideration of the above two points.

DOI： 10.3390/molecules17032446

Synthesis of Dumbbell-Shaped Cyclic RNAs for RNA Interference

Naoko Abe, Hiroshi Abe, Yoshihiro Ito

Current Protocol Nucleic Acid Chemistry page： 1-16 2012

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Papers - ABE Hiroshi