研究者詳細 - 佐塚　隆志

論文 - 佐塚　隆志

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脱炭素社会構築への貢献を目標とした新しい作物育種招待有り

橋本舜平、佐塚隆志

作物研究 67 巻頁： 89 - 92 2022年10月

Enhanced production of gamma-amino acid 3-amino-4-hydroxybenzoic acid by recombinant Corynebacterium glutamicum under oxygen limitation 査読有り国際誌

Kawaguchi Hideo, Hasunuma Tomohisa, Ohnishi Yasuo, Sazuka Takashi, Kondo Akihiko, Ogino Chiaki

MICROBIAL CELL FACTORIES 20 巻 ( 1 ) 2021年12月

Complete and Draft Genome Sequences of Amino Acid-Producing Corynebacterium glutamicum Strains ATCC 21799 and ATCC 31831 and Their Genomic Islands 査読有り

Kawaguchi H, Sazuka T, Kondo A

Microbiol Resour Announc. 9 巻 ( 32 ) 2020年8月

Repeated ethanol fermentation from membrane-concentrated sweet sorghum juice using the flocculating yeast Saccharomyces cerevisiae F118 strain 査読有り

Wijaya H, Sasaki K, Kahar P, Yopi, Kawaguchi H, Sazuka T, Ogino C, Prasetya B, Kondo A

265 巻頁： 542 - 547 2018年10月

Transcriptional switch for programmed cell death in pith parenchyma of sorghum stems 査読有り

Fujimoto M, Sazuka T, Oda Y, Kawahigashi H, Wu J, Takanashi H, Ohnishi T, Yoneda J, Ishimori M, Kajiya-Kanegae H, Hibara K, Ishizuna F, Ebine K, Ueda T, Tokunaga T, Iwata H, Matsumoto T, Kasuga S, Yonemaru J, Tsutsumi N

115 巻 ( 37 ) 頁： E8783 - E8792 2018年9月

Effective usage of sorghum bagasse: Optimization of organosolv pretreatment using 25% 1-butanol and subsequent nanofiltration membrane separation 査読有り

Teramura H, Sasaki K, Oshima T, Kawaguchi H, Ogino C, Sazuka T, Kondo A

252 巻頁： 157 - 164 2018年3月

Sucrose purification and repeated ethanol production from sugars remaining in sweet sorghum juice subjected to a membrane separation process 査読有り

Sasaki K, Tsuge Y, Kawaguchi H, Yasukawa M, Sasaki D, Sazuka T, Kamio E, Ogino C, Matsuyama H, Kondo A

101 巻 ( 15 ) 頁： 6007 - 6014 2017年8月

Heap: a highly sensitive and accurate SNP detection tool for low-coverage high-throughput sequencing data 査読有り

Kobayashi Masaaki, Ohyanagi Hajime, Takanashi Hideki, Asano Satomi, Kudo Toru, Kajiya-Kanegae Hiromi, Nagano Atsushi J., Tainaka Hitoshi, Tokunaga Tsuyoshi, Sazuka Takashi, Iwata Hiroyoshi, Tsutsumi Nobuhiro, Yano Kentaro

DNA RESEARCH 24 巻 ( 4 ) 頁： 397 - 405 2017年8月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：DNA Research

Recent availability of large-scale genomic resources enables us to conduct so called genome-wide association studies (GWAS) and genomic prediction (GP) studies, particularly with next-generation sequencing (NGS) data. The effectiveness of GWAS and GP depends on not only their mathematical models, but the quality and quantity of variants employed in the analysis. In NGS single nucleotide polymorphism (SNP) calling, conventional tools ideally require more reads for higher SNP sensitivity and accuracy. In this study, we aimed to develop a tool, Heap, that enables robustly sensitive and accurate calling of SNPs, particularly with a low coverage NGS data, which must be aligned to the reference genome sequences in advance. To reduce false positive SNPs, Heap determines genotypes and calls SNPs at each site except for sites at the both ends of reads or containing a minor allele supported by only one read. Performance comparison with existing tools showed that Heap achieved the highest F-scores with low coverage (7X) restriction-site associated DNA sequencing reads of sorghum and rice individuals. This will facilitate cost-effective GWAS and GP studies in this NGS era. Code and documentation of Heap are freely available from https://github.com/meiji-bioinf/heap (29 March 2017, date last accessed) and our web site (http://bioinf.mind.meiji.ac.jp/lab/en/tools.html (29 March 2017, date last accessed)).

DOI： 10.1093/dnares/dsx012

Web of Science

Scopus

PubMed

Screening of rice mutants with improved saccharification efficiency results in the identification of CONSTITUTIVE PHOTOMORPHOGENIC 1 and GOLD HULL AND INTERNODE 1 査読有り

Hirano K, Masuda R, Takase W, Morinaka Y, Kawamura M, Takeuchi Y, Takagi H, Yaegashi H, Natsume S, Terauchi R, Kotake T, Matsushita Y, Sazuka T

246 巻 ( 1 ) 頁： 61 - 74 2017年7月

Differences in glucose yield of residues from among varieties of rice, wheat, and sorghum after dilute acid pretreatment. 査読有り

Teramura, H., Sasaki, K., Kawaguchi, H., Matsuda, F., Kikuchi, J., Shirai, T., Sazuka, T., Yamasaki, M., Takumi S., Ogino, C., and Kondo, A.

Biosci Biotech Biochem. 2017年6月

Differences in glucose yield of residues from among varieties of rice, wheat, and sorghum after dilute acid pretreatment 査読有り

Teramura H, Sasaki K, Kawaguchi H, Matsuda F, Kikuchi J, Shirai T, Sazuka T, Yamasaki M, Takumi S, Ogino C, Kondo A

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 81 巻 ( 8 ) 頁： 1650 - 1656 2017年

Growth Properties and Biomass Production in the Hybrid C4 Crop Sorghum bicolor. 査読有り

Tazoe Y, Sazuka T, Yamaguchi M, Saito C, Ikeuchi M, Kanno K, Kojima S, Hirano K, Kitano H, Kasuga S, Endo T, Fukuda H, Makino A.

Plant Cell Physiol. 2016年5月

Organosolv pretreatment of sorghum bagasse using a low concentration of hydrophobic solvents such as 1-butanol or 1-pentanol.

J Biosci Bioeng. 9 巻頁： 27 2016年2月

Nanofiltration concentration of extracellular glutathione produced by engineered Saccharomyces cerevisiae.

Sasaki K, Hara KY, Kawaguchi H, Sazuka T, Ogino C, Kondo A.

J Biosci Bioeng. 2016年1月

リグノセルロース系バイオマスの同時糖化発酵によるフェニル乳酸生産(微生物利用の新展開,口頭セッション) 査読有り

川口,秀夫, 寺村,浩, 中村,聡子, 荻野,千秋, 原,清敬, 蓮沼,誠久, 老沼,研一, 高谷,直樹, 平野,恒, 佐塚,隆志, 北野,英己, 近藤,昭彦

バイオマス科学会議発表論文集 11 巻頁： 35 - 36 2016年1月

記述言語：日本語掲載種別：研究論文（国際会議プロシーディングス）出版者・発行元：一般社団法人日本エネルギー学会

Sorghum bagasse pretreated with diluted acid, which was predominantly composed of glucan (59%) and xylan (7.2%), was used as a lignocellulosic feedstock to produce D-phenyllactic acid (PhLA) by a recombinant Escherichia coli strain expressing phenylpyruvate reductase from Wickerhamia fluorescens. Compared to filter paper hydrolysate, the PhLA yield was reduced by 35% during fermentation with enzymatic hydrolysate of sorghum bagasse as a carbon source, and metabolomics analysis revealed that intracellular levels of erythrose-4-phosphate and phosphoenolpyruvate and NAD(P)H regeneration for PhLA production from glucose markedly reduced. Compared to the separate hydrolysis and fermentation (SHF) with sorghum bagasse hydrolysate, simultaneous saccharification and fermentation (SSF) of sorghum bagasse under glucose limitation conditions yielded 4.8-fold more PhLA with less accumulation of eluted components of p-coumaric acid and aldehydes, which inhibited PhLA fermentation. These results suggest that gradual hydrolysis of sorghum bagasse during SSF reduces the accumulation of both glucose and fermentation inhibitors, collectively leading to increased PhLA yield.

DOI： 10.20550/jiebiomassronbun.11.0_35

CiNii Books

3-Amino-4-hydroxybenzoic acid production from sweet sorghum juice by recombinant <i>Corynebacterium glutamicum</i> 査読有り国際誌

Kawaguchi Hideo, Sasaki Kengo, Uematsu Kouji, Tsuge Yota, Teramura Hiroshi, Okai Naoko, Nakamura-Tsuruta Sachiko, Katsuyama Yohei, Sugai Yoshinori, Ohnishi Yasuo, Hirano Ko, Sazuka Takashi, Ogino Chiaki, Kondo Akihiko

BIORESOURCE TECHNOLOGY 198 巻頁： 410 - 417 2015年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Bioresource Technology

The production of the bioplastic precursor 3-amino-4-hydroxybenzoic acid (3,4-AHBA) from sweet sorghum juice, which contains amino acids and the fermentable sugars sucrose, glucose and fructose, was assessed to address the limitations of producing bio-based chemicals from renewable feedstocks. Recombinant Corynebacterium glutamicum strain KT01 expressing griH and griI derived from Streptomyces griseus produced 3,4-AHBA from the sweet sorghum juice of cultivar SIL-05 at a final concentration (1.0gl-1) that was 5-fold higher than that from pure sucrose. Fractionation of sweet sorghum juice by nanofiltration (NF) membrane separation (molecular weight cut-off 150) revealed that the NF-concentrated fraction, which contained the highest concentrations of amino acids, increased 3,4-AHBA production, whereas the NF-filtrated fraction inhibited 3,4-AHBA biosynthesis. Amino acid supplementation experiments revealed that leucine specifically enhanced 3,4-AHBA production by strain KT01. Taken together, these results suggest that sweet sorghum juice is a potentially suitable feedstock for 3,4-AHBA production by recombinant C. glutamicum.

DOI： 10.1016/j.biortech.2015.09.024

Nanofiltration concentration of extracellular glutathione produced by engineered Saccharomyces cerevisiae.

Sasaki K, Hara KY, Kawaguchi H, Sazuka T, Ogino C, Kondo A.

J Biosci Bioeng. 2015年6月

Repeated ethanol production from sweet sorghum juice concentrated by membrane separation. Bioresour Technol.

Sasaki K, Tsuge Y, Sasaki D, Kawaguchi H, Sazuka T, Ogino C, Kondo A.

Bioresour Technol. 2015年6月

Phenyllactic acid production by simultaneous saccharification and fermentation of pretreated sorghum bagasse.

Kawaguchi H, Teramura H, Uematsu K, Hara KY, Hasunuma T, Hirano K, Sazuka T, Kitano H, Tsuge Y, Kahar P, Niimi-Nakamura S, Oinuma K, Takaya N, Kasuga S, Ogino C, Kondo A.

Bioresour Technol. 2015年4月

Repeated ethanol production from sweet sorghum juice concentrated by membrane separation.

Sasaki K, Tsuge Y, Sasaki D, Kawaguchi H, Sazuka T, Ogino C, Kondo A.

Bioresour Technol. 186 巻頁： 351-355 2015年1月

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