研究者詳細 - 佐塚　隆志

論文 - 佐塚　隆志

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An analysis of sugary endosperm in sorghum: Characterization of mutant phenotypes depending on alleles of the corresponding starch debranching enzyme 査読有り

Hashimoto Shumpei, Okada Satoshi, Araki-Nakamura Satoko, Ohmae-Shinohara Kozue, Miura Kotaro, Kawaguchi Hideo, Ogino Chiaki, Kasuga Shigemitsu, Sazuka Takashi

FRONTIERS IN PLANT SCIENCE 14 巻頁： 1114935 2023年2月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Frontiers in Plant Science

Sorghum is the fifth most important cereal crop. Here we performed molecular genetic analyses of the ‘SUGARY FETERITA’ (SUF) variety, which shows typical sugary endosperm traits (e.g., wrinkled seeds, accumulation of soluble sugars, and distorted starch). Positional mapping indicated that the corresponding gene was located on the long arm of chromosome 7. Within the candidate region of 3.4 Mb, a sorghum ortholog for maize Su1 (SbSu) encoding a starch debranching enzyme ISA1 was found. Sequencing analysis of SbSu in SUF uncovered nonsynonymous single nucleotide polymorphisms (SNPs) in the coding region, containing substitutions of highly conserved amino acids. Complementation of the rice sugary-1 (osisa1) mutant line with the SbSu gene recovered the sugary endosperm phenotype. Additionally, analyzing mutants obtained from an EMS-induced mutant panel revealed novel alleles with phenotypes showing less severe wrinkles and higher Brix scores. These results suggested that SbSu was the corresponding gene for the sugary endosperm. Expression profiles of starch synthesis genes during the grain-filling stage demonstrated that a loss-of-function of SbSu affects the expression of most starch synthesis genes and revealed the fine-tuned gene regulation in the starch synthetic pathway in sorghum. Haplotype analysis using 187 diverse accessions from a sorghum panel revealed the haplotype of SUF showing severe phenotype had not been used among the landraces and modern varieties. Thus, weak alleles (showing sweet and less severe wrinkles), such as in the abovementioned EMS-induced mutants, are more valuable for grain sorghum breeding. Our study suggests that more moderate alleles (e.g. produced by genome editing) should be beneficial for improving grain sorghum.

DOI： 10.3389/fpls.2023.1114935

Web of Science

Scopus

PubMed

ソルガムの雑種強勢メカニズムとバイオマスデザイニング招待有り査読有り

橋本舜平、佐塚隆志

アグリバイオ 7 巻 ( 6 ) 頁： 8 - 12 2023年6月

The dominance model for heterosis explains culm length genetics in a hybrid sorghum variety 査読有り国際誌

Hashimoto Shumpei, Wake Tatsuro, Nakamura Haruki, Minamiyama Masaki, Araki-Nakamura Satoko, Ohmae-Shinohara Kozue, Koketsu Eriko, Okamura Shinnosuke, Miura Kotaro, Kawaguchi Hideo, Kasuga Shigemitsu, Sazuka Takashi

SCIENTIFIC REPORTS 11 巻 ( 1 ) 2021年2月

Sorghum DW1 positively regulates brassinosteroid signaling by inhibiting the nuclear localization of BRASSINOSTEROID INSENSITIVE 2 査読有り

Hirano K, Kawamura M, Araki-Nakamura S, Fujimoto H, Ohmae-Shinohara K, Yamaguchi M, Fujii A, Sasaki H, Kasuga S, Sazuka T

Sci Rep. 7 巻 2017年3月

Sorghum Dw1, an agronomically important gene for lodging resistance, encodes a novel protein involved in cell proliferation. 査読有り

Yamaguchi M, Fujimoto H, Hirano K, Araki-Nakamura S, Ohmae-Shinohara K, Fujii A, Tsunashima M, Song XJ, Ito Y, Nagae R, Wu J, Mizuno H, Yonemaru J, Matsumoto T, Kitano H, Matsuoka M, Kasuga S, Sazuka T.

Sci Rep. 2016年6月

Gibberellin deficiency pleiotropically induces culm bending in sorghum: an insight into sorghum semi-dwarf breeding.

Ordonio RL, Ito Y, Hatakeyama A, Ohmae-Shinohara K, Kasuga S, Tokunaga T, Mizuno H, Kitano H, Matsuoka M, Sazuka T.

Sci Rep. 2014年6月

A rice tryptophan deficient dwarf mutant, tdd1, contains a reduced level of indole acetic acid and develops abnormal flowers and organless embryos 査読有り国際誌

Sazuka Takashi, Kamiya Noriko, Nishimura Takeshi, Ohmae Kozue, Sato Yutaka, Imamura Kohei, Nagato Yasuo, Koshiba Tomokazu, Nagamura Yoshiaki, Ashikari Motoyuki, Kitano Hidemi, Matsuoka Makoto

PLANT JOURNAL 60 巻 ( 2 ) 頁： 227 - 241 2009年10月

Auxin Biosynthesis by the YUCCA Genes in Rice. 査読有り

Yamamoto Y, Kamiya N, Morinaka Y, Matsuoka M and Sazuka T.

Plant Physiol. 143 巻 ( 3 ) 頁： 1362-1371 2007年3月

Production and characterization of auxin-insensitive rice by overexpression of a mutagenized rice IAA protein. 査読有り

Nakamura A, Umemura I, Gomi K, Hasegawa Y, Kitano H, Sazuka T, Matsuoka M.

Plant J. 46 巻 ( 2 ) 頁： 297-306 2006年4月

A proteomic analysis of cyanobacterium, Anabaena sp. strain PCC7120 through two-dimensional gel electrophoresis and amino-terminal sequencing. 招待有り査読有り

Sazuka T.

Photosynth Res. 78 巻 ( 3 ) 頁： 279-291 2003年6月

Towards a proteome project of cyanobacterium Synechocystis sp. strain PCC6803: Linking 130 protein spots with their respective genes. 招待有り査読有り

Sazuka T, Ohara O

Electrophoresis 18 巻 ( 8 ) 頁： 1252-1258 1997年8月

ソルガムを培地成分とした黄麹菌の培養特性

本迫翔, 松本琴音, 鈴木智大, 若井暁, Kahar Prihardi, 森裕太郎, 佐塚隆志, 荻野千秋

日本生物工学会大会講演要旨集 2024年巻頁： 94 - 94 2024年8月

DAC農業の実現にむけた作物改良と評価招待有り査読有り

矢野昌裕, 米丸淳一, 佐塚隆志, 小松晃, 木下俊則, 吉川直樹

育種学研究 26 巻 ( 1 ) 頁： 54 - 60 2024年4月

Utilization of sweet sorghum juice as a carbon source for enhancement of itaconic acid production in engineered<i> Corynebacterium</i><i> glutamicum</i> 査読有り国際共著

Elkasaby, T; Hanh, DD; Kahar, P; Kawaguchi, H; Sazuka, T; Kondo, A; Ogino, C

ENZYME AND MICROBIAL TECHNOLOGY 172 巻頁： 110345 2024年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Enzyme and Microbial Technology

Itaconic acid is a promising biochemical building block that can be used in polymer synthesis. Itaconic acid is currently produced in industry by the natural producer fungus Aspergillus terreus using glucose as a main carbon source. Most research for itaconic acid production using lignocellulosic-based carbon sources was carried out by A. terreus. Engineered Corynebacterium glutamicum strain which can grow in presence of fermentation inhibitors without effect on growth, was used for production of itaconic acid using sweet sorghum juice and bagasse sugar lysate (BSL). BSL contains many inhibitors unlike sorghum juice. C. glutamicum could grow in the media containing both types of lignocellulose-based carbon sources without showing any growth inhibition, however, sorghum juice was better in itaconic acid production than BSL. Different constructed strains of C. glutamicum were used for itaconic acid production, however, C. glutamicum ATCC 13032 pCH-Tad1optAdi1opt strain expressing Adi1/Tad1 genes (trans-pathway) from Ustilago maydis proved to be better in itaconic acid production giving final titer of 8.4 and 4.02 g/L using sweet sorghum juice and BSL as the sole carbon sources by fed-batch fermentation. Our study is the first for production of itaconic acid using sweet sorghum juice and BSL. The present study also proved that C. glutamicum can be used for enhancing itaconic acid production using lignocellulosic-based carbon sources.

DOI： 10.1016/j.enzmictec.2023.110345

Web of Science

Scopus

PubMed

脱炭素社会構築に貢献する草本バイオマスの可能性. 招待有り

佐塚隆志

アグリバイオ 7 巻 ( 6 ) 頁： 6 - 7 2023年6月

ソルガムの雑種強勢メカニズムとバイオマスデザイニング招待有り

橋本舜平、佐塚隆志

アグリバイオ 7 巻 ( 6 ) 頁： 8 - 12 2023年6月

Metabolic engineering for 4-aminophenylalanine production from lignocellulosic biomass by recombinant Escherichia coli. 査読有り

RSC Sustainability 1 巻 ( 4 ) 頁： 1043 - 1054 2023年5月

ソルガム個体群における階層別光合成特性の解析招待有り

日本作物学会講演会要旨集 2023年3月

Utilization of sweet sorghum juice as a carbon source for enhancement of itaconic acid production in engineered Corynebacterium glutamicum 査読有り国際共著

Taghreed Elkasaby T, Hanh DD, Kahar P, Kawaguchi H, Sazuka T, Kondo A, Ogino C.

Enzyme and Microbial Technology 172 巻 ( 1 ) 頁： 110345. - 110345. 2023年1月

Metabolic engineering for 4-aminophenylalanine production from lignocellulosic biomass by recombinant Escherichia coli

Kawaguchi H., Masuo S., Wakai K., Takaya N., Hasunma T., Kaneko T., Okada S., Sazuka T., Ogino C., Kondo A.

RSC Sustainability 1 巻 ( 4 ) 頁： 1043 - 1054 2023年

掲載種別：研究論文（学術雑誌）出版者・発行元：RSC Sustainability

To synthesize a high-performance biopolyimide bioplastic from lignocellulosic feedstock, Escherichia coli was metabolically engineered to produce 4-amino-l-phenylalanine (4APhe) as a diamine monomer. A high-biomass sorghum cultivar was used as the model lignocellulosic feedstock, and the enzymatic hydrolysate was used as a substrate for 4APhe production in fed-batch culture. When using the ldh mutant strain, HKE6027, 4APhe production from glucose was increased by over three-fold compared to the parent strain. This increase was due to the disruption of biosynthetic pathways that produce either acetate or lactate as by-products. Comparative metabolomic analysis revealed increased flux in both the pentose phosphate and shikimate pathways in HKE6027, resulting in the highest yields. However, 5.7 g L−1 of 4APhe was produced from enzymatic hydrolysis by HKE6027, which was 24% lower than that obtained from glucose. The concentrations of 14 potential fermentation inhibitors present in the enzymatic hydrolysate were determined, and their inhibitory effects on both cell growth and 4APhe production were examined. The addition of groups of potential inhibitors present in the enzymatic hydrolysate of sorghum bagasse showed that benzaldehyde- and cinnamic acid derivatives inhibited 4APhe fermentation at low concentrations (half-maximum inhibitor concentration IC50 = 16 mg L−1), whereas furfural and 5-hydroxymethylfurfural, which are well-known fermentation inhibitors, inhibited 4APhe fermentation at relatively high concentrations (IC50 = 640 mg L−1). These results provide insight into the design of metabolic pathways tailored for the utilization of lignocellulosic biomass to produce aromatic compounds through the shikimate pathway.

DOI： 10.1039/d3su00053b

Scopus

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