研究者詳細 - 佐塚　隆志

論文 - 佐塚　隆志

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An analysis of sugary endosperm in sorghum: Characterization of mutant phenotypes depending on alleles of the corresponding starch debranching enzyme 査読有り

Hashimoto Shumpei, Okada Satoshi, Araki-Nakamura Satoko, Ohmae-Shinohara Kozue, Miura Kotaro, Kawaguchi Hideo, Ogino Chiaki, Kasuga Shigemitsu, Sazuka Takashi

FRONTIERS IN PLANT SCIENCE 14 巻頁： 1114935 2023年2月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Frontiers in Plant Science

Sorghum is the fifth most important cereal crop. Here we performed molecular genetic analyses of the ‘SUGARY FETERITA’ (SUF) variety, which shows typical sugary endosperm traits (e.g., wrinkled seeds, accumulation of soluble sugars, and distorted starch). Positional mapping indicated that the corresponding gene was located on the long arm of chromosome 7. Within the candidate region of 3.4 Mb, a sorghum ortholog for maize Su1 (SbSu) encoding a starch debranching enzyme ISA1 was found. Sequencing analysis of SbSu in SUF uncovered nonsynonymous single nucleotide polymorphisms (SNPs) in the coding region, containing substitutions of highly conserved amino acids. Complementation of the rice sugary-1 (osisa1) mutant line with the SbSu gene recovered the sugary endosperm phenotype. Additionally, analyzing mutants obtained from an EMS-induced mutant panel revealed novel alleles with phenotypes showing less severe wrinkles and higher Brix scores. These results suggested that SbSu was the corresponding gene for the sugary endosperm. Expression profiles of starch synthesis genes during the grain-filling stage demonstrated that a loss-of-function of SbSu affects the expression of most starch synthesis genes and revealed the fine-tuned gene regulation in the starch synthetic pathway in sorghum. Haplotype analysis using 187 diverse accessions from a sorghum panel revealed the haplotype of SUF showing severe phenotype had not been used among the landraces and modern varieties. Thus, weak alleles (showing sweet and less severe wrinkles), such as in the abovementioned EMS-induced mutants, are more valuable for grain sorghum breeding. Our study suggests that more moderate alleles (e.g. produced by genome editing) should be beneficial for improving grain sorghum.

DOI： 10.3389/fpls.2023.1114935

Web of Science

Scopus

PubMed

ソルガムの雑種強勢メカニズムとバイオマスデザイニング招待有り査読有り

橋本舜平、佐塚隆志

アグリバイオ 7 巻 ( 6 ) 頁： 8 - 12 2023年6月

The dominance model for heterosis explains culm length genetics in a hybrid sorghum variety 査読有り国際誌

Hashimoto Shumpei, Wake Tatsuro, Nakamura Haruki, Minamiyama Masaki, Araki-Nakamura Satoko, Ohmae-Shinohara Kozue, Koketsu Eriko, Okamura Shinnosuke, Miura Kotaro, Kawaguchi Hideo, Kasuga Shigemitsu, Sazuka Takashi

SCIENTIFIC REPORTS 11 巻 ( 1 ) 2021年2月

Sorghum DW1 positively regulates brassinosteroid signaling by inhibiting the nuclear localization of BRASSINOSTEROID INSENSITIVE 2 査読有り

Hirano K, Kawamura M, Araki-Nakamura S, Fujimoto H, Ohmae-Shinohara K, Yamaguchi M, Fujii A, Sasaki H, Kasuga S, Sazuka T

Sci Rep. 7 巻 2017年3月

Sorghum Dw1, an agronomically important gene for lodging resistance, encodes a novel protein involved in cell proliferation. 査読有り

Yamaguchi M, Fujimoto H, Hirano K, Araki-Nakamura S, Ohmae-Shinohara K, Fujii A, Tsunashima M, Song XJ, Ito Y, Nagae R, Wu J, Mizuno H, Yonemaru J, Matsumoto T, Kitano H, Matsuoka M, Kasuga S, Sazuka T.

Sci Rep. 2016年6月

Gibberellin deficiency pleiotropically induces culm bending in sorghum: an insight into sorghum semi-dwarf breeding.

Ordonio RL, Ito Y, Hatakeyama A, Ohmae-Shinohara K, Kasuga S, Tokunaga T, Mizuno H, Kitano H, Matsuoka M, Sazuka T.

Sci Rep. 2014年6月

A rice tryptophan deficient dwarf mutant, tdd1, contains a reduced level of indole acetic acid and develops abnormal flowers and organless embryos 査読有り国際誌

Sazuka Takashi, Kamiya Noriko, Nishimura Takeshi, Ohmae Kozue, Sato Yutaka, Imamura Kohei, Nagato Yasuo, Koshiba Tomokazu, Nagamura Yoshiaki, Ashikari Motoyuki, Kitano Hidemi, Matsuoka Makoto

PLANT JOURNAL 60 巻 ( 2 ) 頁： 227 - 241 2009年10月

Auxin Biosynthesis by the YUCCA Genes in Rice. 査読有り

Yamamoto Y, Kamiya N, Morinaka Y, Matsuoka M and Sazuka T.

Plant Physiol. 143 巻 ( 3 ) 頁： 1362-1371 2007年3月

Production and characterization of auxin-insensitive rice by overexpression of a mutagenized rice IAA protein. 査読有り

Nakamura A, Umemura I, Gomi K, Hasegawa Y, Kitano H, Sazuka T, Matsuoka M.

Plant J. 46 巻 ( 2 ) 頁： 297-306 2006年4月

A proteomic analysis of cyanobacterium, Anabaena sp. strain PCC7120 through two-dimensional gel electrophoresis and amino-terminal sequencing. 招待有り査読有り

Sazuka T.

Photosynth Res. 78 巻 ( 3 ) 頁： 279-291 2003年6月

Towards a proteome project of cyanobacterium Synechocystis sp. strain PCC6803: Linking 130 protein spots with their respective genes. 招待有り査読有り

Sazuka T, Ohara O

Electrophoresis 18 巻 ( 8 ) 頁： 1252-1258 1997年8月

ソルガムを培地成分とした黄麹菌の培養特性

本迫翔, 松本琴音, 鈴木智大, 若井暁, Kahar Prihardi, 森裕太郎, 佐塚隆志, 荻野千秋

日本生物工学会大会講演要旨集 2024年巻頁： 94 - 94 2024年8月

DAC農業の実現にむけた作物改良と評価招待有り査読有り

矢野昌裕, 米丸淳一, 佐塚隆志, 小松晃, 木下俊則, 吉川直樹

育種学研究 26 巻 ( 1 ) 頁： 54 - 60 2024年4月

Utilization of sweet sorghum juice as a carbon source for enhancement of itaconic acid production in engineered Corynebacterium glutamicum 査読有り国際共著

Elkasaby, T; Hanh, DD; Kahar, P; Kawaguchi, H; Sazuka, T; Kondo, A; Ogino, C

ENZYME AND MICROBIAL TECHNOLOGY 172 巻頁： 110345 2024年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Enzyme and Microbial Technology

Itaconic acid is a promising biochemical building block that can be used in polymer synthesis. Itaconic acid is currently produced in industry by the natural producer fungus Aspergillus terreus using glucose as a main carbon source. Most research for itaconic acid production using lignocellulosic-based carbon sources was carried out by A. terreus. Engineered Corynebacterium glutamicum strain which can grow in presence of fermentation inhibitors without effect on growth, was used for production of itaconic acid using sweet sorghum juice and bagasse sugar lysate (BSL). BSL contains many inhibitors unlike sorghum juice. C. glutamicum could grow in the media containing both types of lignocellulose-based carbon sources without showing any growth inhibition, however, sorghum juice was better in itaconic acid production than BSL. Different constructed strains of C. glutamicum were used for itaconic acid production, however, C. glutamicum ATCC 13032 pCH-Tad1optAdi1opt strain expressing Adi1/Tad1 genes (trans-pathway) from Ustilago maydis proved to be better in itaconic acid production giving final titer of 8.4 and 4.02 g/L using sweet sorghum juice and BSL as the sole carbon sources by fed-batch fermentation. Our study is the first for production of itaconic acid using sweet sorghum juice and BSL. The present study also proved that C. glutamicum can be used for enhancing itaconic acid production using lignocellulosic-based carbon sources.

DOI： 10.1016/j.enzmictec.2023.110345

Web of Science

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PubMed

脱炭素社会構築に貢献する草本バイオマスの可能性. 招待有り

佐塚隆志

アグリバイオ 7 巻 ( 6 ) 頁： 6 - 7 2023年6月

ソルガムの雑種強勢メカニズムとバイオマスデザイニング招待有り

橋本舜平、佐塚隆志

アグリバイオ 7 巻 ( 6 ) 頁： 8 - 12 2023年6月

Metabolic engineering for 4-aminophenylalanine production from lignocellulosic biomass by recombinant Escherichia coli. 査読有り

RSC Sustainability 1 巻 ( 4 ) 頁： 1043 - 1054 2023年5月

ソルガム個体群における階層別光合成特性の解析招待有り

日本作物学会講演会要旨集 2023年3月

Utilization of sweet sorghum juice as a carbon source for enhancement of itaconic acid production in engineered Corynebacterium glutamicum 査読有り国際共著

Taghreed Elkasaby T, Hanh DD, Kahar P, Kawaguchi H, Sazuka T, Kondo A, Ogino C.

Enzyme and Microbial Technology 172 巻 ( 1 ) 頁： 110345. - 110345. 2023年1月

Metabolic engineering for 4-aminophenylalanine production from lignocellulosic biomass by recombinant Escherichia coli

Kawaguchi H., Masuo S., Wakai K., Takaya N., Hasunma T., Kaneko T., Okada S., Sazuka T., Ogino C., Kondo A.

RSC Sustainability 1 巻 ( 4 ) 頁： 1043 - 1054 2023年

掲載種別：研究論文（学術雑誌）出版者・発行元：RSC Sustainability

To synthesize a high-performance biopolyimide bioplastic from lignocellulosic feedstock, Escherichia coli was metabolically engineered to produce 4-amino-l-phenylalanine (4APhe) as a diamine monomer. A high-biomass sorghum cultivar was used as the model lignocellulosic feedstock, and the enzymatic hydrolysate was used as a substrate for 4APhe production in fed-batch culture. When using the ldh mutant strain, HKE6027, 4APhe production from glucose was increased by over three-fold compared to the parent strain. This increase was due to the disruption of biosynthetic pathways that produce either acetate or lactate as by-products. Comparative metabolomic analysis revealed increased flux in both the pentose phosphate and shikimate pathways in HKE6027, resulting in the highest yields. However, 5.7 g L−1 of 4APhe was produced from enzymatic hydrolysis by HKE6027, which was 24% lower than that obtained from glucose. The concentrations of 14 potential fermentation inhibitors present in the enzymatic hydrolysate were determined, and their inhibitory effects on both cell growth and 4APhe production were examined. The addition of groups of potential inhibitors present in the enzymatic hydrolysate of sorghum bagasse showed that benzaldehyde- and cinnamic acid derivatives inhibited 4APhe fermentation at low concentrations (half-maximum inhibitor concentration IC50 = 16 mg L−1), whereas furfural and 5-hydroxymethylfurfural, which are well-known fermentation inhibitors, inhibited 4APhe fermentation at relatively high concentrations (IC50 = 640 mg L−1). These results provide insight into the design of metabolic pathways tailored for the utilization of lignocellulosic biomass to produce aromatic compounds through the shikimate pathway.

DOI： 10.1039/d3su00053b

Scopus

脱炭素社会構築への貢献を目標とした新しい作物育種招待有り

橋本舜平、佐塚隆志

作物研究 67 巻頁： 89 - 92 2022年10月

Enhanced production of gamma-amino acid 3-amino-4-hydroxybenzoic acid by recombinant Corynebacterium glutamicum under oxygen limitation 査読有り国際誌

Kawaguchi Hideo, Hasunuma Tomohisa, Ohnishi Yasuo, Sazuka Takashi, Kondo Akihiko, Ogino Chiaki

MICROBIAL CELL FACTORIES 20 巻 ( 1 ) 2021年12月

Complete and Draft Genome Sequences of Amino Acid-Producing Corynebacterium glutamicum Strains ATCC 21799 and ATCC 31831 and Their Genomic Islands 査読有り

Kawaguchi H, Sazuka T, Kondo A

Microbiol Resour Announc. 9 巻 ( 32 ) 2020年8月

Repeated ethanol fermentation from membrane-concentrated sweet sorghum juice using the flocculating yeast Saccharomyces cerevisiae F118 strain 査読有り

Wijaya H, Sasaki K, Kahar P, Yopi, Kawaguchi H, Sazuka T, Ogino C, Prasetya B, Kondo A

265 巻頁： 542 - 547 2018年10月

Transcriptional switch for programmed cell death in pith parenchyma of sorghum stems 査読有り

Fujimoto M, Sazuka T, Oda Y, Kawahigashi H, Wu J, Takanashi H, Ohnishi T, Yoneda J, Ishimori M, Kajiya-Kanegae H, Hibara K, Ishizuna F, Ebine K, Ueda T, Tokunaga T, Iwata H, Matsumoto T, Kasuga S, Yonemaru J, Tsutsumi N

115 巻 ( 37 ) 頁： E8783 - E8792 2018年9月

Effective usage of sorghum bagasse: Optimization of organosolv pretreatment using 25% 1-butanol and subsequent nanofiltration membrane separation 査読有り

Teramura H, Sasaki K, Oshima T, Kawaguchi H, Ogino C, Sazuka T, Kondo A

252 巻頁： 157 - 164 2018年3月

Sucrose purification and repeated ethanol production from sugars remaining in sweet sorghum juice subjected to a membrane separation process 査読有り

Sasaki K, Tsuge Y, Kawaguchi H, Yasukawa M, Sasaki D, Sazuka T, Kamio E, Ogino C, Matsuyama H, Kondo A

101 巻 ( 15 ) 頁： 6007 - 6014 2017年8月

Heap: a highly sensitive and accurate SNP detection tool for low-coverage high-throughput sequencing data 査読有り

Kobayashi Masaaki, Ohyanagi Hajime, Takanashi Hideki, Asano Satomi, Kudo Toru, Kajiya-Kanegae Hiromi, Nagano Atsushi J., Tainaka Hitoshi, Tokunaga Tsuyoshi, Sazuka Takashi, Iwata Hiroyoshi, Tsutsumi Nobuhiro, Yano Kentaro

DNA RESEARCH 24 巻 ( 4 ) 頁： 397 - 405 2017年8月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：DNA Research

Recent availability of large-scale genomic resources enables us to conduct so called genome-wide association studies (GWAS) and genomic prediction (GP) studies, particularly with next-generation sequencing (NGS) data. The effectiveness of GWAS and GP depends on not only their mathematical models, but the quality and quantity of variants employed in the analysis. In NGS single nucleotide polymorphism (SNP) calling, conventional tools ideally require more reads for higher SNP sensitivity and accuracy. In this study, we aimed to develop a tool, Heap, that enables robustly sensitive and accurate calling of SNPs, particularly with a low coverage NGS data, which must be aligned to the reference genome sequences in advance. To reduce false positive SNPs, Heap determines genotypes and calls SNPs at each site except for sites at the both ends of reads or containing a minor allele supported by only one read. Performance comparison with existing tools showed that Heap achieved the highest F-scores with low coverage (7X) restriction-site associated DNA sequencing reads of sorghum and rice individuals. This will facilitate cost-effective GWAS and GP studies in this NGS era. Code and documentation of Heap are freely available from https://github.com/meiji-bioinf/heap (29 March 2017, date last accessed) and our web site (http://bioinf.mind.meiji.ac.jp/lab/en/tools.html (29 March 2017, date last accessed)).

DOI： 10.1093/dnares/dsx012

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PubMed

Screening of rice mutants with improved saccharification efficiency results in the identification of CONSTITUTIVE PHOTOMORPHOGENIC 1 and GOLD HULL AND INTERNODE 1 査読有り

Hirano K, Masuda R, Takase W, Morinaka Y, Kawamura M, Takeuchi Y, Takagi H, Yaegashi H, Natsume S, Terauchi R, Kotake T, Matsushita Y, Sazuka T

246 巻 ( 1 ) 頁： 61 - 74 2017年7月

Differences in glucose yield of residues from among varieties of rice, wheat, and sorghum after dilute acid pretreatment. 査読有り

Teramura, H., Sasaki, K., Kawaguchi, H., Matsuda, F., Kikuchi, J., Shirai, T., Sazuka, T., Yamasaki, M., Takumi S., Ogino, C., and Kondo, A.

Biosci Biotech Biochem. 2017年6月

Differences in glucose yield of residues from among varieties of rice, wheat, and sorghum after dilute acid pretreatment 査読有り

Teramura H, Sasaki K, Kawaguchi H, Matsuda F, Kikuchi J, Shirai T, Sazuka T, Yamasaki M, Takumi S, Ogino C, Kondo A

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 81 巻 ( 8 ) 頁： 1650 - 1656 2017年

Growth Properties and Biomass Production in the Hybrid C4 Crop Sorghum bicolor. 査読有り

Tazoe Y, Sazuka T, Yamaguchi M, Saito C, Ikeuchi M, Kanno K, Kojima S, Hirano K, Kitano H, Kasuga S, Endo T, Fukuda H, Makino A.

Plant Cell Physiol. 2016年5月

Organosolv pretreatment of sorghum bagasse using a low concentration of hydrophobic solvents such as 1-butanol or 1-pentanol.

J Biosci Bioeng. 9 巻頁： 27 2016年2月

Nanofiltration concentration of extracellular glutathione produced by engineered Saccharomyces cerevisiae.

Sasaki K, Hara KY, Kawaguchi H, Sazuka T, Ogino C, Kondo A.

J Biosci Bioeng. 2016年1月

リグノセルロース系バイオマスの同時糖化発酵によるフェニル乳酸生産(微生物利用の新展開,口頭セッション) 査読有り

川口,秀夫, 寺村,浩, 中村,聡子, 荻野,千秋, 原,清敬, 蓮沼,誠久, 老沼,研一, 高谷,直樹, 平野,恒, 佐塚,隆志, 北野,英己, 近藤,昭彦

バイオマス科学会議発表論文集 11 巻頁： 35 - 36 2016年1月

記述言語：日本語掲載種別：研究論文（国際会議プロシーディングス）出版者・発行元：一般社団法人日本エネルギー学会

Sorghum bagasse pretreated with diluted acid, which was predominantly composed of glucan (59%) and xylan (7.2%), was used as a lignocellulosic feedstock to produce D-phenyllactic acid (PhLA) by a recombinant Escherichia coli strain expressing phenylpyruvate reductase from Wickerhamia fluorescens. Compared to filter paper hydrolysate, the PhLA yield was reduced by 35% during fermentation with enzymatic hydrolysate of sorghum bagasse as a carbon source, and metabolomics analysis revealed that intracellular levels of erythrose-4-phosphate and phosphoenolpyruvate and NAD(P)H regeneration for PhLA production from glucose markedly reduced. Compared to the separate hydrolysis and fermentation (SHF) with sorghum bagasse hydrolysate, simultaneous saccharification and fermentation (SSF) of sorghum bagasse under glucose limitation conditions yielded 4.8-fold more PhLA with less accumulation of eluted components of p-coumaric acid and aldehydes, which inhibited PhLA fermentation. These results suggest that gradual hydrolysis of sorghum bagasse during SSF reduces the accumulation of both glucose and fermentation inhibitors, collectively leading to increased PhLA yield.

DOI： 10.20550/jiebiomassronbun.11.0_35

CiNii Books

3-Amino-4-hydroxybenzoic acid production from sweet sorghum juice by recombinant Corynebacterium glutamicum 査読有り国際誌

Kawaguchi Hideo, Sasaki Kengo, Uematsu Kouji, Tsuge Yota, Teramura Hiroshi, Okai Naoko, Nakamura-Tsuruta Sachiko, Katsuyama Yohei, Sugai Yoshinori, Ohnishi Yasuo, Hirano Ko, Sazuka Takashi, Ogino Chiaki, Kondo Akihiko

BIORESOURCE TECHNOLOGY 198 巻頁： 410 - 417 2015年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Bioresource Technology

The production of the bioplastic precursor 3-amino-4-hydroxybenzoic acid (3,4-AHBA) from sweet sorghum juice, which contains amino acids and the fermentable sugars sucrose, glucose and fructose, was assessed to address the limitations of producing bio-based chemicals from renewable feedstocks. Recombinant Corynebacterium glutamicum strain KT01 expressing griH and griI derived from Streptomyces griseus produced 3,4-AHBA from the sweet sorghum juice of cultivar SIL-05 at a final concentration (1.0gl-1) that was 5-fold higher than that from pure sucrose. Fractionation of sweet sorghum juice by nanofiltration (NF) membrane separation (molecular weight cut-off 150) revealed that the NF-concentrated fraction, which contained the highest concentrations of amino acids, increased 3,4-AHBA production, whereas the NF-filtrated fraction inhibited 3,4-AHBA biosynthesis. Amino acid supplementation experiments revealed that leucine specifically enhanced 3,4-AHBA production by strain KT01. Taken together, these results suggest that sweet sorghum juice is a potentially suitable feedstock for 3,4-AHBA production by recombinant C. glutamicum.

DOI： 10.1016/j.biortech.2015.09.024

Nanofiltration concentration of extracellular glutathione produced by engineered Saccharomyces cerevisiae.

Sasaki K, Hara KY, Kawaguchi H, Sazuka T, Ogino C, Kondo A.

J Biosci Bioeng. 2015年6月

Repeated ethanol production from sweet sorghum juice concentrated by membrane separation. Bioresour Technol.

Sasaki K, Tsuge Y, Sasaki D, Kawaguchi H, Sazuka T, Ogino C, Kondo A.

Bioresour Technol. 2015年6月

Phenyllactic acid production by simultaneous saccharification and fermentation of pretreated sorghum bagasse.

Kawaguchi H, Teramura H, Uematsu K, Hara KY, Hasunuma T, Hirano K, Sazuka T, Kitano H, Tsuge Y, Kahar P, Niimi-Nakamura S, Oinuma K, Takaya N, Kasuga S, Ogino C, Kondo A.

Bioresour Technol. 2015年4月

Repeated ethanol production from sweet sorghum juice concentrated by membrane separation.

Sasaki K, Tsuge Y, Sasaki D, Kawaguchi H, Sazuka T, Ogino C, Kondo A.

Bioresour Technol. 186 巻頁： 351-355 2015年1月

Increased ethanol production from sweet sorghum juice concentrated by a membrane separation process.

Sasaki K, Tsuge Y, Sasaki D, Teramura H, Wakai S, Kawaguchi H, Sazuka T, Ogino C, Kondo A.

Bioresour Technol. 2014年10月

Phenyllactic acid production by simultaneous saccharification and fermentation of pretreated sorghum bagasse.

Kawaguchi H, Teramura H, Uematsu K, Hara KY, Hasunuma T, Hirano K, Sazuka T, Kitano H, Tsuge Y, Kahar P, Niimi-Nakamura S, Oinuma K, Takaya N, Kasuga S, Ogino C, Kondo A.

Bioresour Technol. 2014年4月

From Genome to Breeding: Challenge of Genome-Assisted Breeding of Sorghum.

Sazuka T and Iwata H.

J. Jpn Inst. Enregy 93 巻頁： 429-435 2014年

Thioredoxin h regulates calcium dependent protein kinases in plasma membranes.

Ueoka-Nakanishi, H., Sazuka, T., Nakanishi, Y., Maeshima, M., Mori, H. and Hisabori, T.

FEBS J. 280 巻頁： 3220-3231 2013年

A high-throughput phenotyping system supporting genomic breeding of crop plants 国際誌

Iwata H., Kobayashi M., Ohyanagi H., Yano K., Sazuka T., Tokunaga T., Tsutsumi N.

Proceedings of the SICE Annual Conference 頁： 1778 - 1779 2013年

記述言語：英語掲載種別：研究論文（国際会議プロシーディングス）出版者・発行元：Proceedings of the SICE Annual Conference

High-throughput technologies for determining DNA sequence variations have enabled new genomics-based breeding strategies, such as genomic selection. While genomics technologies are making rapid progress in throughput and cost efficiencies, technologies for measuring plant phenotypes, i.e., "phenotyping", remain inefficient. Phenotyping is now the most time-consuming and error-prone step in genomic-based breeding strategies. To measure plant phenotypes with high efficiency and accuracy, we are developing a high-throughput phenotyping system. The system consists of two main parts: The plant management system using radio-frequency identification device (RFID) tags and the data collection system connected to multiple measuring devices via Bluetooth. The first system enables us to link plants to data on the Internet and to keep records of cultivation management. The second system enables us to collect data measured by multiple devices via Bluetooth connections. With this system, we can aggregate various types of measurement data in an efficient and precise manner. High-throughput phenotyping systems lead to efficient and accurate data collection and will strongly support genomic breeding in crop plants.

Scopus

Mapping and characterization of the rice gene, CLUB-SHAPED EMBRYO3, CLE3. 査読有り

Imamura K, Nagato Y, Matsuoka M, Kitano H, Sazuka T.

Rice Gen. Newslett. 25 巻 ( 1 ) 頁： 27-29 2010年5月

A rice tryptophan deficient dwarf mutant, tdd1, contains a reduced level of indole acetic acid and develops abnormal flowers and organless embryos. 査読有り

Sazuka T, Kamiya N, Nishimura T, Ohmae K, Sato Y, Imamura K, Nagato Y, Koshiba T, Nagamura Y, Ashikari M, Kitano H, Matsuoka M.

Plant J. 60 巻 ( 2 ) 頁： 227-241 2009年10月

Mapping and Analysis of the CLUB-SHAPED EMBRYO1, CLE1. 査読有り

24 巻 ( 1 ) 頁： 22-24 2008年9月

Possible thioredoxin targets of signaling molecules in plasma membranes 査読有り国際誌

Ueoka-Nakanishi Hanayo, Sazuka Takashi, Maeshima Masayoshi, Hisabori Toru

PLANT AND CELL PHYSIOLOGY 48 巻頁： S41 - S41 2007年

Analysis of club-shaped embryol, a loss of function mutant of rice DICER-LIKE1

Imamura Kohei, Imai Hanako, Matsuoka Makoto, Kitano Hidemi, Sazuka Takashi

PLANT AND CELL PHYSIOLOGY 48 巻頁： S36 - S36 2007年

The rice SPINDLY gene functions as a negative regulator of gibberellin signaling by controlling the suppressive function of the DELLA protein, SLR1, and modulating brassinosteroid synthesis. 査読有り

Shimada A, Ueguchi-Tanaka M, Sakamoto T, Fujioka S, Takatsuto S, Yoshida S, Sazuka T, Ashikari, M, Matsuoka M.

Plant J. 48 巻 ( 3 ) 頁： 390-402 2006年11月

Exploring the thioredoxin targets in plant membrane proteins

Ueoka-Nakanishi H, Maeshima M, Sazuka T, Hisabori T

PLANT AND CELL PHYSIOLOGY 47 巻頁： S78 - S78 2006年

The rice mutant dwarf bamboo shoot 1: A leaky mutant of the NACK-type kinesin-like gene can initiate organ primordia but not organ development 査読有り国際誌

Sazuka T, Aichi I, Kawai T, Matsuo N, Kitano H, Matsuoka M

PLANT AND CELL PHYSIOLOGY 46 巻 ( 12 ) 頁： 1934 - 1943 2005年12月

Analysis of the rice mutant dwarf and gladius leaf 1. Aberrant katanin-mediated microtubule organization causes up-regulation of gibberellin biosynthetic genes independently of gibberellin signaling 査読有り国際誌

Komorisono M, Ueguchi-Tanaka M, Aichi I, Hasegawa Y, Ashikari M, Kitano H, Matsuoka M, Sazuka T

PLANT PHYSIOLOGY 138 巻 ( 4 ) 頁： 1982 - 1993 2005年8月

Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana. 査読有り

Shimaoka T, Ohnishi M, Sazuka T, Mitsuhashi N, Hara-Nishimura I, Shimazaki KI, Maeshima M, Yokota A, Tomizawa KI, Mimura T.

Plant Cell Physiol. 45 巻 ( 6 ) 頁： 672-683 2004年6月

A proteomic approach to identification of transmembrane proteins and membrane-anchored proteins of Arabidopsis thaliana by peptide Sequencing 査読有り国際誌

Sazuka T, Keta S, Shiratake K, Yamaki S, Shibata D

DNA RESEARCH 11 巻 ( 2 ) 頁： 101 - 113 2004年4月

SigC, the group 2 sigma factor of RNA polymerase, contributes to the late-stage gene expression and nitrogen promoter recognition in the Cyanobacterium Synechocystis sp. strain PCC 6803. 査読有り

68 巻 ( 3 ) 頁： 477-487 2004年3月

Analysis of dwarf and abnormal leaf morphology 1 (dal1) in rice 招待有り査読有り国際誌

Sazuka T, Komorisono M, Ueguchi-Tanaka M, Kitano H, Ashikari M, Matsuoka M

PLANT AND CELL PHYSIOLOGY 45 巻頁： S113 - S113 2004年

The proteome analysis of the Cyanobacterium Anabaena sp. strain PCC 7120 and classification of the peptides detectable by MALDI-TOF/MS.

Ojima N, Sazuka T, Yamaguchi M, Kuriki T, Akinaga N, Ando E.

51st ASMS Conference on Mass Spectrometry and Allied Topics 頁： 507 2003年

A proteomic approach to identification of transmembrane proteins and membrane-anchored proteins of Arabidopsis thaliana 査読有り国際共著国際誌

Sazuka T, Keta S, Shiratake K, Yamaki S, Shibata D

PLANT AND CELL PHYSIOLOGY 44 巻頁： S18 - S18 2003年

Proteomic analysis of the cyanobacterium Anabaena sp strain PCC7120 with two-dimensional gel electrophoresis and amino-terminal sequencing 査読有り国際誌

Sazuka T

PHOTOSYNTHESIS RESEARCH 78 巻 ( 3 ) 頁： 279 - 291 2003年

Isolation and characterization of intact vacuoles from suspension-cultured Arabidopsis cells

Ohnishi M, Mitsuhashi N, Sekiguchi Y, Sazuka T, Shimazaki K, Nishimura I

PLANT AND CELL PHYSIOLOGY 44 巻頁： S85 - S85 2003年

Proteomics of Arabidopsis thaliana:: a comprehensive analysis of the strongly hydrophobic membrane proteins 査読有り

Sazuka T, Keta S, Shiratake K, Yamaki S, Shibata D

PLANT AND CELL PHYSIOLOGY 43 巻頁： S207 - S207 2002年

Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1 (supplement). 査読有り

N. Nomura, T. Nagase, N. Miyajima, T. Sazuka, A. Tanaka, S. Sato, N. Seki, Y. Kawarabayasi, K. Ishikawa, S. Tabata

DNA research 1 巻頁： 251 - 262 1994年12月

Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1 (supplement).

N. Nomura, N. Miyajima, T. Sazuka, A. Tanaka, Y. Kawarabayasi, S. Sato, T. Nagase, N. Seki, K. Ishikawa, S. Tabata

DNA research : an international journal for rapid publication of reports on genes and genomes 1 巻 ( 1 ) 頁： 47 - 56 1994年

Identification of a developmentally regulated gene in the mouse central nervous system which encodes a novel proline rich protein 査読有り

Takashi Sazuka, Yasuhiro Tomooka, Sandeep Kathju, Yoji Ikawa, Makoto Noda, Sharad Kumar

BBA - Gene Structure and Expression 1132 巻 ( 3 ) 頁： 240 - 248 1992年10月

記述言語：英語掲載種別：研究論文（学術雑誌）

A full length cDNA whose corresponding mRNA is down-regulated during the mouse embryonic brain development was isolated. The cDNA contains a single long open reading frame which could encode a protein with relative molecular mass of 41 kDa. The predicted gene product contains long stretches of prolines towards the NH2-terminus, followed by a leucine/proline rich region. The cDNA probe detected a number of mRNA species in Northern blot analysis. The reverse transcriptase-polymerase chain reaction analysis of mRNA from adult mouse tissues indicated that heart and testis expressed this gene (named NDPP-1) at relatively high levels, while lower levels of mRNA were detected in a number of other tissues. Expression of NDPP-1 was also detected in embryonic carcinoma and pheochromocytoma cell lines, but not in fibroblasts. The cDNA hybridized to genomic DNA from several vertebrates species in Southern blot analysis indicating interspecies conservation of this gene. The interesting pattern of expression of the NDPP-1 gene during mouse brain development and the structure of its putative protein product indicate that this gene may play an important biological role in the development of mouse central nervous system. © 1992.

DOI： 10.1016/0167-4781(92)90156-T

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