研究者詳細 - 松林　嘉克

論文 - 松林　嘉克

分割表示 113 件中 61 - 80 件目／全件表示 >>

A tyrosine-sulfated glycopeptide involved in cellular proliferation and expansion in Arabidopsis. 査読有り

Amano Yukari, Tsubouchi Hiroko, Shinohara Hidefumi, Ogawa Mari, Matsubayashi Yoshikatsu

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 104 巻 ( 46 ) 頁： 18333-18338 2007年11月

Functional immobilization of plant receptor-like kinase onto microbeads towards receptor array construction and receptor-based ligand fishing 査読有り

Shinohara Hidefumi, Matsubayashi Yoshikatsu

PLANT JOURNAL 52 巻 ( 1 ) 頁： 175-184 2007年10月

Identification of ligand binding site of phytosulfokine receptor by on-column photoaffinity labeling 査読有り

Shinohara Hidefumi, Ogawa Mari, Sakagami Youji, Matsubayashi Yoshikatsu

JOURNAL OF BIOLOGICAL CHEMISTRY 282 巻 ( 1 ) 頁： 124-131 2007年1月

Functional immobilization of plant receptor-like kinase onto microbeads toward receptor array construction and receptor-based ligand fishing 査読有り

Shinohara H. and Matsubayashi Y.

Plant J. 52 巻頁： 175-184 2007年

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

Despite the large number of receptor-like kinases (RLKs) in plants, few of them have known specific ligands. Ligand fishing is one of the most challenging post-genomic technologies. Here, we report a strategy for functional immobilization of plant RLKs on microbeads via covalent linkage. Due to the high density of immobilized RLK, ligand-receptor interaction can be visualized at high sensitivity using fluorescent-labeled ligands under the confocal laser scanning microscope. Moreover, using receptor-based affinity chromatography system with RLK microbeads, the ligand of the receptor was directly retrieved at high purity from complex natural samples. Our RLK microbeads and receptor-based ligand fishing approach is a feasible alternative to conventional forward genetics and bioassay-based biochemical purification for identification of novel ligand-receptor pairs in plants.

Identification of ligand binding site of phytosulfokine receptor by on-column photoaffinity labeling. 査読有り

Shinohara H, Ogawa M, Sakagami Y, and Matsubayashi Y.

J. Biol. Chem. 282 巻頁： 124-131 2007年

記述言語：英語掲載種別：研究論文（学術雑誌）

Phytosulfokine (PSK), an endogenous 5-amino-acid secreted peptide in plants, affects cellular potential for growth via binding to PSKR1, a member of the leucine-rich repeat receptor kinase (LRR-RK) family. PSK interacts with PSKR1 in a highly specific manner with a nanomolar dissociation constant. However, it is not known which residues in the PSKR1 extracellular domain constitute the ligand-binding pocket. Here, we identified the PSK-binding domain of carrot PSKR1 (DcPSKR1) by photoaffinity labeling. We cross-linked the photoactivatable PSK analog [125I]Ne-[(4-azidosalicyl)Lys5]PSK with DcPSKR1 using UV irradiation, and mapped the cross-linked region using chemical and enzymatic fragmentation. We also established a novel メon-column photoaffinity labelingモ methodology that allows repeated incorporation of the photoaffinity label to increase the efficiency of the photoaffinity cross-linking reactions. We purified a labeled DcPSKR1 tryptic fragment using anti-PSK antibodies, and identified a peptide fragment that corresponds to the 15-amino-acid Glu503-Lys517 region of DcPSKR1 by MALDI-TOF MS. Deletion of Glu503-Lys517 completely abolishes the ligand-binding activity of DcPSKR1. This region is in the island domain flanked by extracellular LRRs, indicating that this domain forms a ligand-binding pocket that directly interacts with PSK.

Functional analysis of phytosulfokine receptor homologs in Arabidopsis 査読有り

Tsubouchi Hiroko, Shinohara Hidefumi, Ogawa Mari, Sakagami Youji, Matsubayashi Yoshikatsu

PLANT AND CELL PHYSIOLOGY 48 巻頁： S26-S26 2007年

Identification of ligand binding site of phytosulfokine by on-column photoaffinity labeling 査読有り

Shmohara Hidefumi, Ogawa Mari, Sakagami Youji, Matsubayashi Yoshikatsu

PLANT AND CELL PHYSIOLOGY 48 巻頁： S26-S26 2007年

Disruption and overexpression of Arabidopsis phytosulfokine receptor gene affects cellular longevity and potential for growth 査読有り

Matsubayashi Yoshikatsu, Ogawa Mari, Kihara Hitomi, Niwa Masaaki, Sakagami Youji

PLANT PHYSIOLOGY 142 巻 ( 1 ) 頁： 45-53 2006年9月

Peptide hormones in plants 招待有り

Matsubayashi Y. and Sakagami Y.

Annu. Rev. Plant Biol. 57 巻頁： 649-674 2006年

担当区分：筆頭著者記述言語：英語

In recent years, numerous biochemical and genetic studies have demonstrated that peptide signaling plays a greater than anticipated role in various aspects of plant growth and development. A substantial proportion of these peptides are secretory and act as local signals mediating cell-to-cell communication. Specific receptors for several peptides were identified as being membrane-localized receptor kinases, the largest family of receptor-like molecules in plants. These findings illustrate the importance of peptide signaling in the regulation of plant growth, functions that were previously ascribed to the combined action of small lipophilic compounds referred to as メtraditional plant hormones.モ Here, we outline recent advances in the current understanding of biologically active peptides in plants, currently regarded as a new class of plant hormones.

Disruption and overexpression of Arabidopsis phytosulfokine receptor gene affects cellular longevity and potential for growth. 査読有り

Matsubayashi, Y., Ogawa, M., Kihara, H., Niwa, M., and Sakagami, Y.

Plant Physiol. 142 巻頁： 45-53 2006年

記述言語：英語掲載種別：研究論文（学術雑誌）

Phytosulfokine (PSK), a 5-amino-acid sulfated peptide that has been identified in conditioned medium of plant cell cultures, promotes cellular growth in vitro via binding to the membrane-localized PSK receptor. Here, we report that loss-of-function and gain-of-function mutations of the Arabidopsis PSK receptor (AtPSKR1) alter cellular longevity and potential for growth without interfering with basic morphogenesis of plants. Although mutant pskr1-1 plants exhibit morphologically normal growth until 3 weeks after germination, individual pskr1-1 cells gradually lose their potential to form calluses as tissues mature. Shortly after a pskr1-1 callus forms, it loses potential for growth, resulting in formation of a smaller callus than the wild-type. Leaves of pskr1-1 plants exhibit premature senescence after bolting. Leaves of AtPSKR1ox plants exhibit greater longevity and significantly greater potential for callus formation than leaves of wild-type plants, irrespective of their age. Calluses derived from AtPSKR1ox plants maintain their potential for growth longer than wild-type calluses. Combined with the present finding that PSK precursor genes are more strongly expressed in mature plant parts than in immature plant parts, the available evidence indicates that PSK signaling affects cellular longevity and potential for growth, and thereby exerts a pleiotropic effect on cultured tissue in response to environmental hormonal conditions.

Identification and functional characterization of phytosulfokine receptor using a ligand-based approach 招待有り査読有り

Matsubayashi Y., Shinohara H. and Ogawa M.

Chem. Rec. 6 巻頁： 356-364 2006年

Peptide hormones in plants 査読有り

Matsubayashi Yoshikatsu, Sakagami Youji

ANNUAL REVIEW OF PLANT BIOLOGY 57 巻頁： 649-674 2006年

Identification and functional characterization of phytosulfokine receptor using a ligand-based approach 査読有り

Matsubayashi Yoshikatsu, Shinohara Hidefumi, Ogawa Mari

CHEMICAL RECORD 6 巻 ( 6 ) 頁： 356-364 2006年

Phytosulfokine 査読有り

Matsubayashi Yoshikatsu, Sakagami Youji

HANDBOOK OF BIOLOGICALLY ACTIVE PEPTIDES 頁： 29-32 2006年

Ion-selective enrichment of tyrosine-sulfated peptides from complex protein digests. 査読有り

Amano Y., Shinohara H., Sakagami Y. and Matsubayashi Y.

Anal. Biochem. 346 巻頁： 124-131 2005年

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

We have developed a novel procedure for concentrating sulfated peptides, as a front end to mass spectrometric analysis, based on ion-selective interaction of sulfate ions with anion exchangers. Ions with a higher charge and smaller solvated ion radius, such as sulfate ions, have higher retention in an ion exchanger, due to their greater degree of coulombic interactions. We tested the effectiveness of this approach for enrichment and identification of sulfated peptides, using a tryptic digest of bovine serum albumin spiked with model sulfated peptide (molar ratio, 20:1), and using a tryptic digest of bovine fibrinogen. Sulfated peptides are identified by mass spectrometry, in which both the molecular ion and its specific fragment ion produced by facile loss of SO3 are detected. In both experiments, sulfated peptides were strongly retained on the anion exchanger, and were eluted by higher concentrations of competing ion with minimum contamination of non-sulfated peptides. Using this procedure, we determined that the 13-amino-acid C-terminal peptide of the minor gユ chain of bovine fibrinogen contains sulfated tyrosine.

Further analogues of plant peptide hormone phytosulfokine-alpha (PSK-alpha) and their biological evaluation 査読有り

Bahyrycz A., Matsubayashi Y., Ogawa M., Sakagami Y. and Konopinska D.

J. Pept Sci. 11 巻頁： 589-592 2005年

Two stimulatory effects of the peptidyl growth factor phytosulfokine during somatic embryogenesis in Japanese larch (Larix leptolepis Gordon). 査読有り

Umehara M., Ogita S., Sasamoto H., Eun CH., Matsubayashi Y., Sakagami Y. and Kamada H.

Plant Sci. 169 巻頁： 901-907 2005年

Plant peptide hormone phytosulfokine (PSK-alpha) and its analogues 査読有り

Bahyrycz Agata, Matsubayashi Yoshikatsu, Ogawa Mari, Sakagami Youji, Konopinska Danuta

Peptides 2004, Proceedings 頁： 784-785 2005年

Chemical nursing: phytosulfokine improves genetic transformation efficiency by promoting the proliferation of surviving cells on selective media. 査読有り

Matsubayashi Y., Goto T. and Sakagami Y.

Plant Cell Rep. 23 巻頁： 155-158 2004年

Plant peptide hormone phytosulfokine (PSK-alpha): synthesis of new analogues and their biological evaluation 査読有り

Bahyrycz A., Matsubayashi Y., Ogawa M., Sakagami Y. and Konopinska D.

J. Pept Sci. 10 巻頁： 462-469 2004年

　前のページ - 次のページ　

<松林 嘉克>

論文 - 松林 嘉克

<松林　嘉克>

論文 - 松林　嘉克