研究者詳細 - 松林　嘉克

論文 - 松林　嘉克

分割表示 >> ／全件表示 113 件中 1 - 113 件目

Arabidopsis SBT5.2 and SBT1.7 subtilases mediate C-terminal cleavage of flg22 epitope from bacterial flagellin. 査読有り国際誌

Matsui S, Noda S, Kuwata K, Nomoto M, Tada Y, Shinohara H, Matsubayashi Y

Nature communications 15 巻 ( 1 ) 頁： 3762 2024年5月

Peptide ligand-mediated trade-off between plant growth and stress response 査読有り国際誌

Ogawa-Ohnishi, M; Yamashita, T; Kakita, M; Nakayama, T; Ohkubo, Y; Hayashi, Y; Yamashita, Y; Nomura, T; Noda, S; Shinohara, H; Matsubayashi, Y

SCIENCE 378 巻 ( 6616 ) 頁： 175 - 180 2022年10月

A type 2C protein phosphatase activates high-affinity nitrate uptake by dephosphorylating NRT2.1 査読有り国際誌

Ohkubo, Y; Kuwata, K; Matsubayashi, Y

NATURE PLANTS 7 巻 ( 3 ) 頁： 310 - + 2021年3月

A peptide hormone required for Casparian strip diffusion barrier formation in Arabidopsis roots 査読有り国際誌

Nakayama, T; Shinohara, H; Tanaka, M; Baba, K; Ogawa-Ohnishi, M; Matsubayashi, Y

SCIENCE 355 巻 ( 6322 ) 頁： 284 - 286 2017年1月

Perception of root-derived peptides by shoot LRR-RKs mediates systemic N-demand signaling 査読有り

Tabata Ryo, Sumida Kumiko, Yoshii Tomoaki, Ohyama Kentaro, Shinohara Hidefumi, Matsubayashi Yoshikatsu

SCIENCE 346 巻 ( 6207 ) 頁： 343-346 2014年10月

Secreted Peptide Signals Required for Maintenance of Root Stem Cell Niche in Arabidopsis 査読有り

Matsuzaki Yo, Ogawa-Ohnishi Mari, Mori Ayaka, Matsubayashi Yoshikatsu

SCIENCE 329 巻 ( 5995 ) 頁： 1065-1067 2010年8月

Arabidopsis CLV3 peptide directly binds CLV1 ectodomain 査読有り

Ogawa Mari, Shinohara Hidefumi, Sakagami Youji, Matsubayashi Yoshikatsu

SCIENCE 319 巻 ( 5861 ) 頁： 294-294 2008年1月

An LRR receptor kinase involved in the perception of a plant peptide hormone, phytosulfokine 査読有り

Matsubayashi Y., Ogawa M., Morita A. and Sakagami Y.

SCIENCE 296 巻頁： 1470-1472 2002年5月

An Activity-Based Proteomics with Two-Dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE) for Identifying Target Proteases in Arabidopsis Apoplastic Fluid. 招待有り査読有り国際誌

Matsui S, Matsubayashi Y

Bio-protocol 15 巻 ( 5 ) 頁： e5226 2025年3月

Phototropin switches between cis- and trans-autophosphorylation in light-induced chloroplast relocation in Marchantia polymorpha. 査読有り国際誌

Noguchi M, Noda S, Matsubayashi Y, Kodama Y

The Plant journal : for cell and molecular biology 121 巻 ( 1 ) 頁： e17183 2025年1月

Integration of shoot-derived polypeptide signals by root TGA transcription factors is essential for survival under fluctuating nitrogen environments 査読有り

Ryutaro Kobayashi, Yuri Ohkubo, Mai Izumi, Ryosuke Ota, Keiko Yamada, Yoko Hayashi, Yasuko Yamashita, Saki Noda, Mari Ogawa-Ohnishi, Yoshikatsu Matsubayashi

NATURE COMMUNICATIONS 15 巻 ( 1 ) 頁： 6903 2024年8月

IMA peptides regulate root nodulation and nitrogen homeostasis by providing iron according to internal nitrogen status 査読有り

Ito, M; Tajima, Y; Ogawa-Ohnishi, M; Nishida, H; Nosaki, S; Noda, M; Sotta, N; Kawade, K; Kamiya, T; Fujiwara, T; Matsubayashi, Y; Suzaki, T

NATURE COMMUNICATIONS 15 巻 ( 1 ) 2024年1月

Evaluation of Direct Ligand-Receptor Interactions by Photoaffinity Labeling. 招待有り査読有り国際誌

Shinohara H, Matsubayashi Y

Methods in molecular biology (Clifton, N.J.) 2731 巻頁： 231 - 240 2024年

Extraction of Apoplastic Peptides for the Structural Elucidation of Mature Peptide Hormones in Arabidopsis. 招待有り査読有り国際誌

Ogawa-Ohnishi M, Matsubayashi Y

Methods in molecular biology (Clifton, N.J.) 2731 巻頁： 81 - 87 2024年

Arabinogalactan protein polysaccharide chains are required for normal biogenesis of plasmodesmata 査読有り

Okawa, R; Hayashi, Y; Yamashita, Y; Matsubayashi, Y; Ogawa-Ohnishi, M

PLANT JOURNAL 113 巻 ( 3 ) 頁： 493 - 503 2023年2月

Genomic analysis of an ultrasmall freshwater green alga, Medakamo hakoo 査読有り

Kato, S; Misumi, O; Maruyama, S; Nozaki, H; Tsujimoto-Inui, Y; Takusagawa, M; Suzuki, S; Kuwata, K; Noda, S; Ito, N; Okabe, Y; Sakamoto, T; Yagisawa, F; Matsunaga, TM; Matsubayashi, Y; Yamaguchi, H; Kawachi, M; Kuroiwa, H; Kuroiwa, T; Matsunaga, S

COMMUNICATIONS BIOLOGY 6 巻 ( 1 ) 2023年1月

Identification of Receptors of Plant Peptide Hormones by Photoaffinity Labeling

Shinohara, H; Matsubayashi, Y

JOURNAL OF SYNTHETIC ORGANIC CHEMISTRY JAPAN 78 巻 ( 7 ) 頁： 713 - 722 2020年7月

Shoot-to-root mobile CEPD-like 2 integrates shoot nitrogen status to systemically regulate nitrate uptake in Arabidopsis 査読有り国際誌

Ota, R; Ohkubo, Y; Yamashita, Y; Ogawa-Ohnishi, M; Matsubayashi, Y

NATURE COMMUNICATIONS 11 巻 ( 1 ) 2020年1月

Screening and identification of a non-peptide antagonist for the peptide hormone receptor in Arabidopsis

Shinohara, H; Yasue, N; Onuki, T; Kondoh, Y; Yoshida, M; Matsubayashi, Y

COMMUNICATIONS BIOLOGY 2 巻 2019年2月

PLENTY, a hydroxyproline O-arabinosyltransferase, negatively regulates root nodule symbiosis in Lotus japonicus

Yoro, E; Nishida, H; Ogawa-Ohnishi, M; Yoshida, C; Suzaki, T; Matsubayashi, Y; Kawaguchi, M

JOURNAL OF EXPERIMENTAL BOTANY 70 巻 ( 2 ) 頁： 507 - 517 2019年1月

Lateral Inhibition by a Peptide Hormone-Receptor Cascade during Arabidopsis Lateral Root Founder Cell Formation

Toyokura, K; Goh, T; Shinohara, H; Shinoda, A; Kondo, Y; Okamoto, Y; Uehara, T; Fujimoto, K; Okushima, Y; Ikeyama, Y; Nakajima, K; Mimura, T; Tasaka, M; Matsubayashi, Y; Fukaki, H

DEVELOPMENTAL CELL 48 巻 ( 1 ) 頁： 64 - + 2019年1月

Pectin RG-I rhamnosyltransferases represent a novel plant-specific glycosyltransferase family

Takenaka, Y; Kato, K; Ogawa-Ohnishi, M; Tsuruhama, K; Kajiura, H; Yagyu, K; Takeda, A; Takeda, Y; Kunieda, T; Hara-Nishimura, I; Kuroha, T; Nishitani, K; Matsubayashi, Y; Ishimizu, T

NATURE PLANTS 4 巻 ( 9 ) 頁： 669 - 676 2018年9月

Exploring peptide hormones in plants: identification of four peptide hormone-receptor pairs and two post-translational modification enzymes

Matsubayashi, Y

PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES 94 巻 ( 2 ) 頁： 59 - 74 2018年2月

Analysis of PLETHORA Gradient Formation by Secreted Peptides During Root Development

Shinohara, H; Matsubayashi, Y

MORPHOGEN GRADIENTS: METHODS AND PROTOCOLS 1863 巻頁： 155 - 164 2018年

Shoot-to-root mobile polypeptides involved in systemic regulation of nitrogen acquisition 査読有り国際誌

Ohkubo, Y; Tanaka, M; Tabata, R; Ogawa-Ohnishi, M; Matsubayashi, Y

NATURE PLANTS 3 巻 ( 4 ) 頁： 17029 2017年4月

Cryptic bioactivity capacitated by synthetic hybrid plant peptides 査読有り国際誌

Hirakawa, Y; Shinohara, H; Welke, K; Irle, S; Matsubayashi, Y; Torii, KU; Uchida, N

NATURE COMMUNICATIONS 8 巻頁： 14318 2017年2月

Expression of Plant Receptor Kinases in Tobacco BY-2 Cells 査読有り

Shinohara, H; Matsubayashi, Y

PLANT RECEPTOR KINASES 1621 巻頁： 29 - 35 2017年

Photoaffinity Labeling of Plant Receptor Kinases 査読有り

Shinohara, H; Matsubayashi, Y

PLANT RECEPTOR KINASES 1621 巻頁： 59 - 68 2017年

Long-distance peptide signaling essential for nutrient homeostasis in plants.

Okamoto S, Tabata R, Matsubayashi Y

Current opinion in plant biology 34 巻頁： 35-40 2016年12月

Loss of function at RAE2, a previously unidentified EPFL, is required for awnlessness in cultivated Asian rice.

Bessho-Uehara K, Wang DR, Furuta T, Minami A, Nagai K, Gamuyao R, Asano K, Angeles-Shim RB, Shimizu Y, Ayano M, Komeda N, Doi K, Miura K, Toda Y, Kinoshita T, Okuda S, Higashiyama T, Nomoto M, Tada Y, Shinohara H, Matsubayashi Y, Greenberg A, Wu J, Yasui H, Yoshimura A, Mori H, McCouch SR, Ashikari M

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 113 巻 ( 32 ) 頁： 8969-74 2016年8月

Identification of three LRR-RKs involved in perception of root meristem growth factor in Arabidopsis. 査読有り

Shinohara H, Mori A, Yasue N, Sumida K, Matsubayashi Y

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 113 巻 ( 14 ) 頁： 3897-902 2016年4月

A comprehensive strategy for identifying long-distance mobile peptides in xylem sap. 査読有り

Okamoto S, Suzuki T, Kawaguchi M, Higashiyama T, Matsubayashi Y

The Plant journal : for cell and molecular biology 84 巻 ( 3 ) 頁： 611-20 2015年11月

An Arabidopsis thaliana copper-sensitive mutant suggests a role of phytosulfokine in ethylene production 査読有り

Wu Tao, Kamiya Takehiro, Yumoto Hiroko, Sotta Naoyuki, Katsushi Yamaguchi, Shigenobu Shuji, Matsubayashi Yoshikatsu, Fujiwara Toru

JOURNAL OF EXPERIMENTAL BOTANY 66 巻 ( 13 ) 頁： 3657-3667 2015年7月

A cascade of arabinosyltransferases controls shoot meristem size in tomato 査読有り

Xu Cao, Liberatore Katie L., MacAlister Cora A., Huang Zejun, Chu Yi-Hsuan, Jiang Ke, Brooks Christopher, Ogawa-Ohnishi Mari, Xiong Guangyan, Pauly Markus, Van Eck Joyce, Matsubayashi Yoshikatsu, van der Knaap Esther, Lippman Zachary B.

NATURE GENETICS 47 巻 ( 7 ) 頁： 784-+ 2015年7月

An Arabidopsis thaliana copper-sensitive mutant suggests a role of phytosulfokine in ethylene production. 査読有り

Wu T, Kamiya T, Yumoto H, Sotta N, Katsushi Y, Shigenobu S, Matsubayashi Y, Fujiwara T

Journal of experimental botany 66 巻 ( 13 ) 頁： 3657-67 2015年7月

Reevaluation of the CLV3-receptor interaction in the shoot apical meristem: dissection of the CLV3 signaling pathway from a direct ligand-binding point of view 査読有り

Shinohara Hidefumi, Matsubayashi Yoshikatsu

PLANT JOURNAL 82 巻 ( 2 ) 頁： 328-336 2015年4月

Reevaluation of the CLV3-receptor interaction in the shoot apical meristem: dissection of the CLV3 signaling pathway from a direct ligand-binding point of view. 査読有り

Shinohara H, Matsubayashi Y

The Plant journal : for cell and molecular biology 82 巻 ( 2 ) 頁： 328-36 2015年4月

Identification of three potent hydroxyproline O-galactosyltransferases in Arabidopsis 査読有り

Ogawa-Ohnishi Mari, Matsubayashi Yoshikatsu

PLANT JOURNAL 81 巻 ( 5 ) 頁： 736-746 2015年3月

Identification of three potent hydroxyproline O-galactosyltransferases in Arabidopsis. 査読有り

Ogawa-Ohnishi M, Matsubayashi Y

The Plant journal : for cell and molecular biology 81 巻 ( 5 ) 頁： 736-46 2015年3月

CLE6 expression recovers gibberellin deficiency to promote shoot growth in Arabidopsis 査読有り

Bidadi Haniyeh, Matsuoka Keita, Sage-Ono Kimiyo, Fukushima Jun, Pitaksaringkarn Weerasak, Asahina Masashi, Yamaguchi Shinjiro, Sawa Shinichiro, Fukuda Hiroo, Matsubayashi Yoshikatsu, Ono Michiyuki, Satoh Shinobu

PLANT JOURNAL 78 巻 ( 2 ) 頁： 241-252 2014年4月

Posttranslationally Modified Small-Peptide Signals in Plants 査読有り

Matsubayashi Yoshikatsu

ANNUAL REVIEW OF PLANT BIOLOGY, VOL 65 65 巻頁： 385-413 2014年

Posttranslationally modified small-peptide signals in plants. 査読有り

Matsubayashi Y

Annual review of plant biology 65 巻頁： 385-413 2014年

Identification of three hydroxyproline O-arabinosyltransferases in Arabidopsis thaliana 査読有り

Ogawa-Ohnishi Mari, Matsushita Wataru, Matsubayashi Yoshikatsu

NATURE CHEMICAL BIOLOGY 9 巻 ( 11 ) 頁： 726-+ 2013年11月

A Novel Pollen-Pistil Interaction Conferring High-Temperature Tolerance during Reproduction via CLE45 Signaling 査読有り

Endo Satoshi MS, Shinohara Hidefumi, Matsubayashi Yoshikatsu, Fukuda Hiroo

CURRENT BIOLOGY 23 巻 ( 17 ) 頁： 1670-1676 2013年9月

Root-derived CLE glycopeptides control nodulation by direct binding to HAR1 receptor kinase 査読有り

Okamoto Satoru, Shinohara Hidefumi, Mori Tomoko, Matsubayashi Yoshikatsu, Kawaguchi Masayoshi

NATURE COMMUNICATIONS 4 巻 2013年8月

Chemical Synthesis of Arabidopsis CLV3 Glycopeptide Reveals the Impact of Hydroxyproline Arabinosylation on Peptide Conformation and Activity 査読有り

Shinohara Hidefumi, Matsubayashi Yoshikatsu

PLANT AND CELL PHYSIOLOGY 54 巻 ( 3 ) 頁： 369-374 2013年3月

Identification of three hydroxyproline O-arabinosyltransferases in Arabidopsis thaliana 査読有り

Ogawa-Ohnishi M., Matsushita W., Matsubayashi Y.

NATURE CHEMICAL BIOLOGY 9 巻頁： 726-730 2013年1月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

Hydroxyproline (Hyp) O-arabinosylation is a posttranslational modification that is prominent in extracellular glycoproteins in plants. Hyp O-arabinosylation is generally found in these glycoproteins in the form of linear oligoarabinoside chains and plays a key role in their function by contributing to conformational stability. However, Hyp O-arabinosyltransferase (HPAT), a key enzyme that catalyzes the transfer of the L-arabinose to the hydroxyl group of Hyp residues, has remained undiscovered. Here, we purified and identified Arabidopsis HPAT as a Golgi-localized transmembrane protein that is structurally similar to the glycosyltransferase GT8 family. Loss-of-function mutations in HPAT genes cause pleiotropic phenotypes that include enhanced hypocotyl elongation, defects in cell wall thickening, early flowering, early senescence, and impaired pollen tube growth. Our results indicate essential roles of Hyp O-arabinosylation in both vegetative and reproductive growth in plants.

Chemical synthesis of Arabidopsis CLV3 glycopeptide reveals the impact of hydroxyproline arabinosylation on peptide conformation and activity. 査読有り

Shinohara H. and Matsubayashi Y.

Plant Cell Physiol. 54 巻頁： 369-374 2013年

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

Arabinosylation of hydroxyproline (Hyp) is a posttranslational modification often found in secreted peptide signals in plants. The physiological importance of this modification was highlighted by the finding that CLAVATA3 (CLV3), a key peptide signal for regulating the fate of stem cells in shoot apical meristem (SAM) in Arabidopsis, contains three L-arabinose residues linked via linear β-1,2-linkages. However, understanding the functions and properties of arabinosylated peptides have been hindered by difficulties in synthesizing the complex arabinose chain. Here we report the stereoselective total synthesis of β-1,2-linked tri-arabinosylated CLV3 peptide ([Ara3]CLV3). Chemically synthesized [Ara3]CLV3 restricted stem cell activity more effectively than did unmodified CLV3 peptide. Comparison of mono-, di- and tri-arabinosylated CLV3 glycopeptides revealed that the biological activity increased progressively as arabinose chain length increased. Thus, arabinose chain length of CLV3 is important for its biological activity. NMR spectroscopy and NOE-based structure calculations further revealed the structural impact of the arabinose chain on peptide conformation. The arabinose chain of [Ara3]CLV3 extends toward the C-terminal end of the peptide, and its non-reducing end is positioned proximal to the peptide backbone. Consequently, the arabinose chain causes distinct distortion in the C-terminal half of the peptide in a highly directional manner. The established synthetic route of [Ara3]CLV3 will greatly contribute to our understanding of the biology and biochemistry of arabinosylated peptide signals in plants.

Biochemical mapping of a ligand-binding domain within Arabidopsis BAM1 reveals diversified ligand recognition mechanisms of plant LRR-RKs 査読有り

Shinohara Hidefumi, Moriyama Yuji, Ohyama Kentaro, Matsubayashi Yoshikatsu

PLANT JOURNAL 70 巻 ( 5 ) 頁： 845-854 2012年6月

Recent progress in research on small post-translationally modified peptide signals in plants 査読有り

Matsubayashi Yoshikatsu

GENES TO CELLS 17 巻 ( 1 ) 頁： 1-10 2012年1月

Biochemical mapping of a ligand-binding domain within Arabidopsis BAM1 reveals diversified ligand recognition mechanisms of plant LRR-RKs. 査読有り

Shinohara H., Moriyama Y., Ohyama K., Matsubayashi Y.

Plant J. 70 巻頁： 845-854 2012年

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

Leucine-rich repeat receptor kinases (LRR-RKs) comprise the largest subfamily of the transmembrane receptor kinases in plants. In several LRR-RKs, a loop-out region called an island domain that intercepts the extracellular tandem LRRs at a position near the transmembrane domain constitutes the ligand-binding pocket, but the absence of the island domain in numerous LRR-RKs raises questions about which domain specifically recognizes the corresponding ligands in non-island domain-carrying LRR-RKs. Here, we determined by photoaffinity labeling followed by chemical and enzymatic digestion that BAM1, a CLV1/BAM family LRR-RK whose extracellular domain is comprised of 22 consecutive LRRs, directly interacts with the small peptide ligand CLE9 at the LRR6-8 region that is relatively distal from the transmembrane domain. Multiple sequence alignment and homology modeling revealed that the inner concave side of LRR6-8 of the CLV1/BAM family LRR-RKs is slightly deviatory from the LRR consensus. Supporting our findings, the clv1-4 mutant carries a missense mutation at the inner concave side of LRR6 of CLV1, and introduction of the corresponding mutation into BAM1 resulted in complete loss of ligand-binding activity. Our results indicate that ligand recognition mechanisms of plant LRR-RKs are more complex and diversified than anticipated.

Post-Translational Modifications in Secreted Peptide Hormones in Plants 査読有り

Matsubayashi Yoshikatsu

PLANT AND CELL PHYSIOLOGY 52 巻 ( 1 ) 頁： 5-13 2011年1月

Arabinosylated glycopeptide hormones: new insights into CLAVATA3 structure 査読有り

Shinohara Hidefumi, Matsubayashi Yoshikatsu

CURRENT OPINION IN PLANT BIOLOGY 13 巻 ( 5 ) 頁： 515-519 2010年10月

Identification of tyrosylprotein sulfotransferase in Arabidopsis 査読有り

Komori Ryota, Amano Yukari, Ogawa-Ohnishi Mari, Matsubayashi Yoshikatsu

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 106 巻 ( 35 ) 頁： 15067-15072 2009年9月

A glycopeptide regulating stem cell fate in Arabidopsis thaliana 査読有り

Ohyama Kentaro, Shinohara Hidefumi, Ogawa-Ohnishi Mari, Matsubayashi Yoshikatsu

NATURE CHEMICAL BIOLOGY 5 巻 ( 8 ) 頁： 578-580 2009年8月

Involvement of Phytosulfokine in the Attenuation of Stress Response during the Transdifferentiation of Zinnia Mesophyll Cells into Tracheary Elements 査読有り

Motose Hiroyasu, Iwamoto Kuninori, Endo Satoshi, Demura Taku, Sakagami Youji, Matsubayashi Yoshikatsu, Moore Kevin L., Fukuda Hiroo

PLANT PHYSIOLOGY 150 巻 ( 1 ) 頁： 437-447 2009年5月

Non-cell-autonomous control of vascular stem cell fate by a CLE peptide/receptor system 査読有り

Hirakawa Yuki, Shinohara Hidefumi, Kondo Yuki, Inoue Asuka, Nakanomyo Ikuko, Ogawa Mari, Sawa Shinichiro, Ohashi-Ito Kyoko, Matsubayashi Yoshikatsu, Fukuda Hiroo

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 105 巻 ( 39 ) 頁： 15208-15213 2008年9月

Identification of a biologically active, small, secreted peptide in Arabidopsis by in silico gene screening, followed by LC-MS-based structure analysis 査読有り

Ohyama Kentaro, Ogawa Mari, Matsubayashi Yoshikatsu

PLANT JOURNAL 55 巻 ( 1 ) 頁： 152-160 2008年7月

Non-cell-autonomous control of vascular stem cell fate by a CLE peptide/receptor system. 査読有り

Hirakawa Y., Shinohara H., Kondo Y., Inoue A., Nakanomyo I., Ogawa M., Sawa S., Ohashi-Ito K., Matsubayashi Y. and Fukuda H.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 105 巻頁： 15208-15213 2008年

Identification of a biologically active, small, secreted peptide in Arabidopsis by in silico gene screening, followed by LC-MS-based structure analysis. 査読有り

Ohyama K, Ogawa M, Matsubayashi Y.

Plant J. 55 巻頁： 152-160 2008年

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

Peptidomics is a challenging field in which to create a link between genomic information and biological function through biochemical analysis of expressed peptides, including precise identification of post-translational modifications and proteolytic processing. We found that secreted peptides in Arabidopsis plants diffuse into the medium of whole-plant submerged cultures, and can be effectively identified by o-chlorophenol extraction followed by LC-MS analysis. Using this system, we first confirmed that a 12-amino-acid mature CLE44 peptide accumulated at a considerable level in the culture medium of transgenic plants overexpressing CLE44. Next, using an in silico approach, we identified a novel gene family encoding small secreted peptides that exhibit significant sequence similarity within the C-terminal short conserved domain. We determined that the mature peptide encoded by At1g47485, a member of this gene family, is a 15-amino-acid peptide containing two hydroxyproline residues derived from the conserved domain. This peptide, which we have named CEP1, is mainly expressed in the lateral root primordia and, when overexpressed or externally applied, significantly arrests root growth. CEP1 is a candidate for a novel peptide plant hormone.

A tyrosine-sulfated glycopeptide involved in cellular proliferation and expansion in Arabidopsis. 査読有り

Amano Yukari, Tsubouchi Hiroko, Shinohara Hidefumi, Ogawa Mari, Matsubayashi Yoshikatsu

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 104 巻 ( 46 ) 頁： 18333-18338 2007年11月

Functional immobilization of plant receptor-like kinase onto microbeads towards receptor array construction and receptor-based ligand fishing 査読有り

Shinohara Hidefumi, Matsubayashi Yoshikatsu

PLANT JOURNAL 52 巻 ( 1 ) 頁： 175-184 2007年10月

Identification of ligand binding site of phytosulfokine receptor by on-column photoaffinity labeling 査読有り

Shinohara Hidefumi, Ogawa Mari, Sakagami Youji, Matsubayashi Yoshikatsu

JOURNAL OF BIOLOGICAL CHEMISTRY 282 巻 ( 1 ) 頁： 124-131 2007年1月

Functional immobilization of plant receptor-like kinase onto microbeads toward receptor array construction and receptor-based ligand fishing 査読有り

Shinohara H. and Matsubayashi Y.

Plant J. 52 巻頁： 175-184 2007年

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

Despite the large number of receptor-like kinases (RLKs) in plants, few of them have known specific ligands. Ligand fishing is one of the most challenging post-genomic technologies. Here, we report a strategy for functional immobilization of plant RLKs on microbeads via covalent linkage. Due to the high density of immobilized RLK, ligand-receptor interaction can be visualized at high sensitivity using fluorescent-labeled ligands under the confocal laser scanning microscope. Moreover, using receptor-based affinity chromatography system with RLK microbeads, the ligand of the receptor was directly retrieved at high purity from complex natural samples. Our RLK microbeads and receptor-based ligand fishing approach is a feasible alternative to conventional forward genetics and bioassay-based biochemical purification for identification of novel ligand-receptor pairs in plants.

Identification of ligand binding site of phytosulfokine receptor by on-column photoaffinity labeling. 査読有り

Shinohara H, Ogawa M, Sakagami Y, and Matsubayashi Y.

J. Biol. Chem. 282 巻頁： 124-131 2007年

記述言語：英語掲載種別：研究論文（学術雑誌）

Phytosulfokine (PSK), an endogenous 5-amino-acid secreted peptide in plants, affects cellular potential for growth via binding to PSKR1, a member of the leucine-rich repeat receptor kinase (LRR-RK) family. PSK interacts with PSKR1 in a highly specific manner with a nanomolar dissociation constant. However, it is not known which residues in the PSKR1 extracellular domain constitute the ligand-binding pocket. Here, we identified the PSK-binding domain of carrot PSKR1 (DcPSKR1) by photoaffinity labeling. We cross-linked the photoactivatable PSK analog [125I]Ne-[(4-azidosalicyl)Lys5]PSK with DcPSKR1 using UV irradiation, and mapped the cross-linked region using chemical and enzymatic fragmentation. We also established a novel メon-column photoaffinity labelingモ methodology that allows repeated incorporation of the photoaffinity label to increase the efficiency of the photoaffinity cross-linking reactions. We purified a labeled DcPSKR1 tryptic fragment using anti-PSK antibodies, and identified a peptide fragment that corresponds to the 15-amino-acid Glu503-Lys517 region of DcPSKR1 by MALDI-TOF MS. Deletion of Glu503-Lys517 completely abolishes the ligand-binding activity of DcPSKR1. This region is in the island domain flanked by extracellular LRRs, indicating that this domain forms a ligand-binding pocket that directly interacts with PSK.

Functional analysis of phytosulfokine receptor homologs in Arabidopsis 査読有り

Tsubouchi Hiroko, Shinohara Hidefumi, Ogawa Mari, Sakagami Youji, Matsubayashi Yoshikatsu

PLANT AND CELL PHYSIOLOGY 48 巻頁： S26-S26 2007年

Identification of ligand binding site of phytosulfokine by on-column photoaffinity labeling 査読有り

Shmohara Hidefumi, Ogawa Mari, Sakagami Youji, Matsubayashi Yoshikatsu

PLANT AND CELL PHYSIOLOGY 48 巻頁： S26-S26 2007年

Disruption and overexpression of Arabidopsis phytosulfokine receptor gene affects cellular longevity and potential for growth 査読有り

Matsubayashi Yoshikatsu, Ogawa Mari, Kihara Hitomi, Niwa Masaaki, Sakagami Youji

PLANT PHYSIOLOGY 142 巻 ( 1 ) 頁： 45-53 2006年9月

Identification and functional characterization of phytosulfokine receptor using a ligand-based approach 招待有り査読有り

Matsubayashi Y., Shinohara H. and Ogawa M.

Chem. Rec. 6 巻頁： 356-364 2006年

Peptide hormones in plants 招待有り

Matsubayashi Y. and Sakagami Y.

Annu. Rev. Plant Biol. 57 巻頁： 649-674 2006年

担当区分：筆頭著者記述言語：英語

In recent years, numerous biochemical and genetic studies have demonstrated that peptide signaling plays a greater than anticipated role in various aspects of plant growth and development. A substantial proportion of these peptides are secretory and act as local signals mediating cell-to-cell communication. Specific receptors for several peptides were identified as being membrane-localized receptor kinases, the largest family of receptor-like molecules in plants. These findings illustrate the importance of peptide signaling in the regulation of plant growth, functions that were previously ascribed to the combined action of small lipophilic compounds referred to as メtraditional plant hormones.モ Here, we outline recent advances in the current understanding of biologically active peptides in plants, currently regarded as a new class of plant hormones.

Disruption and overexpression of Arabidopsis phytosulfokine receptor gene affects cellular longevity and potential for growth. 査読有り

Matsubayashi, Y., Ogawa, M., Kihara, H., Niwa, M., and Sakagami, Y.

Plant Physiol. 142 巻頁： 45-53 2006年

記述言語：英語掲載種別：研究論文（学術雑誌）

Phytosulfokine (PSK), a 5-amino-acid sulfated peptide that has been identified in conditioned medium of plant cell cultures, promotes cellular growth in vitro via binding to the membrane-localized PSK receptor. Here, we report that loss-of-function and gain-of-function mutations of the Arabidopsis PSK receptor (AtPSKR1) alter cellular longevity and potential for growth without interfering with basic morphogenesis of plants. Although mutant pskr1-1 plants exhibit morphologically normal growth until 3 weeks after germination, individual pskr1-1 cells gradually lose their potential to form calluses as tissues mature. Shortly after a pskr1-1 callus forms, it loses potential for growth, resulting in formation of a smaller callus than the wild-type. Leaves of pskr1-1 plants exhibit premature senescence after bolting. Leaves of AtPSKR1ox plants exhibit greater longevity and significantly greater potential for callus formation than leaves of wild-type plants, irrespective of their age. Calluses derived from AtPSKR1ox plants maintain their potential for growth longer than wild-type calluses. Combined with the present finding that PSK precursor genes are more strongly expressed in mature plant parts than in immature plant parts, the available evidence indicates that PSK signaling affects cellular longevity and potential for growth, and thereby exerts a pleiotropic effect on cultured tissue in response to environmental hormonal conditions.

Peptide hormones in plants 査読有り

Matsubayashi Yoshikatsu, Sakagami Youji

ANNUAL REVIEW OF PLANT BIOLOGY 57 巻頁： 649-674 2006年

Identification and functional characterization of phytosulfokine receptor using a ligand-based approach 査読有り

Matsubayashi Yoshikatsu, Shinohara Hidefumi, Ogawa Mari

CHEMICAL RECORD 6 巻 ( 6 ) 頁： 356-364 2006年

Phytosulfokine 査読有り

Matsubayashi Yoshikatsu, Sakagami Youji

HANDBOOK OF BIOLOGICALLY ACTIVE PEPTIDES 頁： 29-32 2006年

Two stimulatory effects of the peptidyl growth factor phytosulfokine during somatic embryogenesis in Japanese larch (Larix leptolepis Gordon). 査読有り

Umehara M., Ogita S., Sasamoto H., Eun CH., Matsubayashi Y., Sakagami Y. and Kamada H.

Plant Sci. 169 巻頁： 901-907 2005年

Ion-selective enrichment of tyrosine-sulfated peptides from complex protein digests. 査読有り

Amano Y., Shinohara H., Sakagami Y. and Matsubayashi Y.

Anal. Biochem. 346 巻頁： 124-131 2005年

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

We have developed a novel procedure for concentrating sulfated peptides, as a front end to mass spectrometric analysis, based on ion-selective interaction of sulfate ions with anion exchangers. Ions with a higher charge and smaller solvated ion radius, such as sulfate ions, have higher retention in an ion exchanger, due to their greater degree of coulombic interactions. We tested the effectiveness of this approach for enrichment and identification of sulfated peptides, using a tryptic digest of bovine serum albumin spiked with model sulfated peptide (molar ratio, 20:1), and using a tryptic digest of bovine fibrinogen. Sulfated peptides are identified by mass spectrometry, in which both the molecular ion and its specific fragment ion produced by facile loss of SO3 are detected. In both experiments, sulfated peptides were strongly retained on the anion exchanger, and were eluted by higher concentrations of competing ion with minimum contamination of non-sulfated peptides. Using this procedure, we determined that the 13-amino-acid C-terminal peptide of the minor gユ chain of bovine fibrinogen contains sulfated tyrosine.

Further analogues of plant peptide hormone phytosulfokine-alpha (PSK-alpha) and their biological evaluation 査読有り

Bahyrycz A., Matsubayashi Y., Ogawa M., Sakagami Y. and Konopinska D.

J. Pept Sci. 11 巻頁： 589-592 2005年

Plant peptide hormone phytosulfokine (PSK-alpha) and its analogues 査読有り

Bahyrycz Agata, Matsubayashi Yoshikatsu, Ogawa Mari, Sakagami Youji, Konopinska Danuta

Peptides 2004, Proceedings 頁： 784-785 2005年

Chemical nursing: phytosulfokine improves genetic transformation efficiency by promoting the proliferation of surviving cells on selective media. 査読有り

Matsubayashi Y., Goto T. and Sakagami Y.

Plant Cell Rep. 23 巻頁： 155-158 2004年

Plant peptide hormone phytosulfokine (PSK-alpha): synthesis of new analogues and their biological evaluation 査読有り

Bahyrycz A., Matsubayashi Y., Ogawa M., Sakagami Y. and Konopinska D.

J. Pept Sci. 10 巻頁： 462-469 2004年

Phytosulfokine stimulates somatic embryogenesis in Cryptomeria japonica. 査読有り

Igasaki T., Akashi N., Ujino-Ihara T., Matsubayashi Y., Sakagami Y. and Shinohara K.

Plant Cell Physiol. 44 巻頁： 1412-1416 2003年

Phytosulfokaine-α,a peptidyl plant grouth factor,stimulates cell cycle progression in carrot nonembryogenic cells.

Plant Biotechnol 20 巻頁： 195-205 2003年

Ligand-receptor pairs in plant peptide signaling. 招待有り査読有り

Matsubayashi Y.

J. Cell Sci. 116 巻頁： 3863-3870 2003年

植物細胞の増殖を制御するペプチド

松林嘉克

2001年7月

ペプチド性植物細胞増殖因子に関する研究(総説). 招待有り

松林嘉克

日本農芸化学会誌 75 巻頁： 1275-1281 2001年1月

Peptide signals and their receptors in higher plant. 招待有り

"Matsubayashi Y., Yang H. and Sakagami Y."

Trends in Plant Science 6 巻頁： 573-577 2001年1月

Diversity of arabidopsis genes encoding precursors for phytosulfokine,a peptide growth factor. 査読有り

Plant Physiol. 127 巻頁： 842-851 2001年

Peptide signals and their receptors in higher plants

Trends in Plant Science 6 巻頁： 573-577 2001年

Peptide growth factor phytosulfokine-a contributes to pollen population effect.

"Chen Y.F., Matsubayashi Y. and Sakagami Y."

Planta 211 巻頁： 752-755 2000年1月

Existence of a plant tyrosylprotein sulfotransferase: novel plant enzyme catalyzing tyrosine O-sulfation of preprophytosulfokine variants in vitro.

"Hanai H., Nakayama D., Yang H., Matsubayashi Y., Hirota Y. and Sakagami Y."

FEBS Letters 470 巻頁： 97-101 2000年1月

"Rapid and efficient Agrobacterium infection-mediated transient gene expression in rice Oc cells and its application for expression and suppression of OsPSK cDNA for a precursor to phytosulfokine-a, a peptide plant growth factor."

"Yang H., Morita A., Matsubayashi Y., Nakamura K. and Sakagami Y."

Plant and Cell Physiology 41 巻頁： 811-816 2000年1月

"Molecular cloning and characterization of OsPSK, a gene encoding a precursor for phytosulfokine-α, required for rice cell proliferation."

"Yang H., Matsubayashi Y., Hanai H., Nakamura K. and Sakagami Y."

Plant Molecular Biology 44 巻頁： 635-647 2000年1月

"Phytosulfokine-α, a peptide growth factor found in higher plants: Its structure, functions, precursor and receptors." 招待有り

"Yang H., Matsubayashi Y., Hanai H. and Sakagami Y."

Plant and Cell Physiology 41 巻頁： 825-830 2000年1月

120- and 160-kDa receptors for endogenous mitogenic peptide, phytosulfokine-alpha, in rice plasma membranes. 査読有り

J. Biol. Chem. 275 巻頁： 15520-15525 2000年

A secreted peptide growth factor, phytosulfokine,acting as a stimulatory factor of carrot somatic embryo formation. 査読有り

Plant Cell Physiol. 41 巻頁： 27-32 2000年

"Promotive effects of the peptidyl plant growth factor, phytosulfokine-a, on the growth and chlorophyll content of Arabidopsis seedlings under high night-time temperature conditions."

"Yamakawa S., Matsubayashi Y. Sakagami Y., Kamada H. and Satoh S."

"Bioscience, Biotechnology and Biochemistry" 63 巻頁： 2240-2243 1999年1月

"Stimulatory effects of a novel peptidyl plant growth factor, phytosulfokine-a, on the adventitious bud formation from callus of Antirrhium majus."

"Yang G., Shen T., Kobayashi Y., Matsubayashi Y., Sakagami Y. and Kamada H."

Plant Biotechnology 16 巻頁： 231-234 1999年1月

Oryza sativa PSK gene encodes a precursor ofphytosulfokine-alpha, a sulfated peptide growthfactor found in plants. 査読有り

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 96 巻頁： 13560-13565 1999年

Characterization of specific binding sites for a mitogenic sulfated peptide, phytosulfokine-alpha, in the plasma membrane fraction derived from Oryza sativa L. 査読有り

Eur. J. Biochem. 262 巻頁： 666-671 1999年

Preparation and characterization of fully active biotinylated analogs of phytosulfokine-alpha. 査読有り

Biosci. Biotechnol. Biochem. 63 巻頁： 1847-1849 1999年

The endogenous sulfated pentapeptide, phytosulfokine-alpha, stimulates tracheary element differentiation of isolated mesophyll cells of Zinnia elegans. 査読有り

Plant Physiol. 120 巻頁： 1043-1048 1999年

Physiological relationships between auxin, cytokinin, and a peptide growth factor, phytosulfokine-α, in stimulation of asparagus cell proliferation.

Planta 207 巻頁： 559-565 1999年

Phytosulfokine-alpha, a peptidyl plant growth factor, stimulates somatic embryogenesis in carrot. 査読有り

J. Exp. Bot. 50 巻頁： 1123-1128 1999年

"The promotive effects of a peptidyl plant growth factor, phytosulfokine-α,on the formation of adventitious roots and expression of a gene for a root-specific cystain in cucumber hypocotyls."

"Yamakawa S., Sakuta C., Matsubayshi Y., Sakagami Y., Kamada H. and Satoh S."

Journal of Plant Research 111 巻頁： 453-458 1998年1月

Differential induction by methyl jasmonate of genes encoding ornithine decarboxylase and other enzymes involved in nicotine biosynthesis in tobacco cell cultures.

"Imanishi S., Hashizume K., Nakakita M., Kojima H., Matsubayashi Y., Hashimoto T., Sakagami Y., Yamada Y. and Nakamura K."

Plant Molecular Biology 38 巻頁： 1101-1111 1998年1月

Promotion by a peptidyl growth factor, phytosulfokine-α, of chlorophyll formation in etiolated cotyledon of cucumber

Yamakawa S., Matsubayashi Y., Sakagami Y., Kamada H. and Satoh S.

Biosci. Biotech. Biochem. 62 巻頁： 2441-2443 1998年

Effect of medium ammonium-nitrate ratio on competence for cell division induced by phytosulfokine-alpha. 査読有り

Plant Cell Rep. 17 巻頁： 368-372 1998年

植物細胞の増殖を誘導するペプチド性因子. 招待有り

"松林嘉克,坂神洋次"

化学と生物 35 巻頁： 6-8 1997年1月

植物細胞増殖因子(総説). 招待有り

"松林嘉克,坂神洋次"

植物の化学調節 32 巻頁： 160-171 1997年1月

Phytosulfokine-alpha, a sulfated pentapeptide, stimulates the proliferation of rice cells by means of specific high-and low-affinity binding sites. 査読有り

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 94 巻頁： 13357-13362 1997年

植物細胞増殖因子(ミニレビュー). 招待有り

"松林嘉克,坂神洋次"

日本農芸化学会誌 70 巻頁： 588-590 1996年1月

Phytosulfokine, sulfated peptides that induce the proliferation of single cells of Asparagus officinalis L. 査読有り

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 93 巻頁： 7623-7627 1996年

Active fragments and analogs of the plaant growth factor, phytosulfokine : Structure-activity relationships. 査読有り

Biochem. Biophys. Res. Commun. 225 巻頁： 209-214 1996年

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