研究者詳細 - 松林　嘉克

論文 - 松林　嘉克

分割表示 113 件中 41 - 60 件目／全件表示 >>

Posttranslationally Modified Small-Peptide Signals in Plants 査読有り

Matsubayashi Yoshikatsu

ANNUAL REVIEW OF PLANT BIOLOGY, VOL 65 65 巻頁： 385-413 2014年

Posttranslationally modified small-peptide signals in plants. 査読有り

Matsubayashi Y

Annual review of plant biology 65 巻頁： 385-413 2014年

Identification of three hydroxyproline O-arabinosyltransferases in Arabidopsis thaliana 査読有り

Ogawa-Ohnishi Mari, Matsushita Wataru, Matsubayashi Yoshikatsu

NATURE CHEMICAL BIOLOGY 9 巻 ( 11 ) 頁： 726-+ 2013年11月

A Novel Pollen-Pistil Interaction Conferring High-Temperature Tolerance during Reproduction via CLE45 Signaling 査読有り

Endo Satoshi MS, Shinohara Hidefumi, Matsubayashi Yoshikatsu, Fukuda Hiroo

CURRENT BIOLOGY 23 巻 ( 17 ) 頁： 1670-1676 2013年9月

Root-derived CLE glycopeptides control nodulation by direct binding to HAR1 receptor kinase 査読有り

Okamoto Satoru, Shinohara Hidefumi, Mori Tomoko, Matsubayashi Yoshikatsu, Kawaguchi Masayoshi

NATURE COMMUNICATIONS 4 巻 2013年8月

Chemical Synthesis of Arabidopsis CLV3 Glycopeptide Reveals the Impact of Hydroxyproline Arabinosylation on Peptide Conformation and Activity 査読有り

Shinohara Hidefumi, Matsubayashi Yoshikatsu

PLANT AND CELL PHYSIOLOGY 54 巻 ( 3 ) 頁： 369-374 2013年3月

Identification of three hydroxyproline O-arabinosyltransferases in Arabidopsis thaliana 査読有り

Ogawa-Ohnishi M., Matsushita W., Matsubayashi Y.

NATURE CHEMICAL BIOLOGY 9 巻頁： 726-730 2013年1月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

Hydroxyproline (Hyp) O-arabinosylation is a posttranslational modification that is prominent in extracellular glycoproteins in plants. Hyp O-arabinosylation is generally found in these glycoproteins in the form of linear oligoarabinoside chains and plays a key role in their function by contributing to conformational stability. However, Hyp O-arabinosyltransferase (HPAT), a key enzyme that catalyzes the transfer of the L-arabinose to the hydroxyl group of Hyp residues, has remained undiscovered. Here, we purified and identified Arabidopsis HPAT as a Golgi-localized transmembrane protein that is structurally similar to the glycosyltransferase GT8 family. Loss-of-function mutations in HPAT genes cause pleiotropic phenotypes that include enhanced hypocotyl elongation, defects in cell wall thickening, early flowering, early senescence, and impaired pollen tube growth. Our results indicate essential roles of Hyp O-arabinosylation in both vegetative and reproductive growth in plants.

Chemical synthesis of Arabidopsis CLV3 glycopeptide reveals the impact of hydroxyproline arabinosylation on peptide conformation and activity. 査読有り

Shinohara H. and Matsubayashi Y.

Plant Cell Physiol. 54 巻頁： 369-374 2013年

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

Arabinosylation of hydroxyproline (Hyp) is a posttranslational modification often found in secreted peptide signals in plants. The physiological importance of this modification was highlighted by the finding that CLAVATA3 (CLV3), a key peptide signal for regulating the fate of stem cells in shoot apical meristem (SAM) in Arabidopsis, contains three L-arabinose residues linked via linear β-1,2-linkages. However, understanding the functions and properties of arabinosylated peptides have been hindered by difficulties in synthesizing the complex arabinose chain. Here we report the stereoselective total synthesis of β-1,2-linked tri-arabinosylated CLV3 peptide ([Ara3]CLV3). Chemically synthesized [Ara3]CLV3 restricted stem cell activity more effectively than did unmodified CLV3 peptide. Comparison of mono-, di- and tri-arabinosylated CLV3 glycopeptides revealed that the biological activity increased progressively as arabinose chain length increased. Thus, arabinose chain length of CLV3 is important for its biological activity. NMR spectroscopy and NOE-based structure calculations further revealed the structural impact of the arabinose chain on peptide conformation. The arabinose chain of [Ara3]CLV3 extends toward the C-terminal end of the peptide, and its non-reducing end is positioned proximal to the peptide backbone. Consequently, the arabinose chain causes distinct distortion in the C-terminal half of the peptide in a highly directional manner. The established synthetic route of [Ara3]CLV3 will greatly contribute to our understanding of the biology and biochemistry of arabinosylated peptide signals in plants.

Biochemical mapping of a ligand-binding domain within Arabidopsis BAM1 reveals diversified ligand recognition mechanisms of plant LRR-RKs 査読有り

Shinohara Hidefumi, Moriyama Yuji, Ohyama Kentaro, Matsubayashi Yoshikatsu

PLANT JOURNAL 70 巻 ( 5 ) 頁： 845-854 2012年6月

Recent progress in research on small post-translationally modified peptide signals in plants 査読有り

Matsubayashi Yoshikatsu

GENES TO CELLS 17 巻 ( 1 ) 頁： 1-10 2012年1月

Biochemical mapping of a ligand-binding domain within Arabidopsis BAM1 reveals diversified ligand recognition mechanisms of plant LRR-RKs. 査読有り

Shinohara H., Moriyama Y., Ohyama K., Matsubayashi Y.

Plant J. 70 巻頁： 845-854 2012年

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

Leucine-rich repeat receptor kinases (LRR-RKs) comprise the largest subfamily of the transmembrane receptor kinases in plants. In several LRR-RKs, a loop-out region called an island domain that intercepts the extracellular tandem LRRs at a position near the transmembrane domain constitutes the ligand-binding pocket, but the absence of the island domain in numerous LRR-RKs raises questions about which domain specifically recognizes the corresponding ligands in non-island domain-carrying LRR-RKs. Here, we determined by photoaffinity labeling followed by chemical and enzymatic digestion that BAM1, a CLV1/BAM family LRR-RK whose extracellular domain is comprised of 22 consecutive LRRs, directly interacts with the small peptide ligand CLE9 at the LRR6-8 region that is relatively distal from the transmembrane domain. Multiple sequence alignment and homology modeling revealed that the inner concave side of LRR6-8 of the CLV1/BAM family LRR-RKs is slightly deviatory from the LRR consensus. Supporting our findings, the clv1-4 mutant carries a missense mutation at the inner concave side of LRR6 of CLV1, and introduction of the corresponding mutation into BAM1 resulted in complete loss of ligand-binding activity. Our results indicate that ligand recognition mechanisms of plant LRR-RKs are more complex and diversified than anticipated.

Post-Translational Modifications in Secreted Peptide Hormones in Plants 査読有り

Matsubayashi Yoshikatsu

PLANT AND CELL PHYSIOLOGY 52 巻 ( 1 ) 頁： 5-13 2011年1月

Arabinosylated glycopeptide hormones: new insights into CLAVATA3 structure 査読有り

Shinohara Hidefumi, Matsubayashi Yoshikatsu

CURRENT OPINION IN PLANT BIOLOGY 13 巻 ( 5 ) 頁： 515-519 2010年10月

Identification of tyrosylprotein sulfotransferase in Arabidopsis 査読有り

Komori Ryota, Amano Yukari, Ogawa-Ohnishi Mari, Matsubayashi Yoshikatsu

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 106 巻 ( 35 ) 頁： 15067-15072 2009年9月

A glycopeptide regulating stem cell fate in Arabidopsis thaliana 査読有り

Ohyama Kentaro, Shinohara Hidefumi, Ogawa-Ohnishi Mari, Matsubayashi Yoshikatsu

NATURE CHEMICAL BIOLOGY 5 巻 ( 8 ) 頁： 578-580 2009年8月

Involvement of Phytosulfokine in the Attenuation of Stress Response during the Transdifferentiation of Zinnia Mesophyll Cells into Tracheary Elements 査読有り

Motose Hiroyasu, Iwamoto Kuninori, Endo Satoshi, Demura Taku, Sakagami Youji, Matsubayashi Yoshikatsu, Moore Kevin L., Fukuda Hiroo

PLANT PHYSIOLOGY 150 巻 ( 1 ) 頁： 437-447 2009年5月

Non-cell-autonomous control of vascular stem cell fate by a CLE peptide/receptor system 査読有り

Hirakawa Yuki, Shinohara Hidefumi, Kondo Yuki, Inoue Asuka, Nakanomyo Ikuko, Ogawa Mari, Sawa Shinichiro, Ohashi-Ito Kyoko, Matsubayashi Yoshikatsu, Fukuda Hiroo

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 105 巻 ( 39 ) 頁： 15208-15213 2008年9月

Identification of a biologically active, small, secreted peptide in Arabidopsis by in silico gene screening, followed by LC-MS-based structure analysis 査読有り

Ohyama Kentaro, Ogawa Mari, Matsubayashi Yoshikatsu

PLANT JOURNAL 55 巻 ( 1 ) 頁： 152-160 2008年7月

Non-cell-autonomous control of vascular stem cell fate by a CLE peptide/receptor system. 査読有り

Hirakawa Y., Shinohara H., Kondo Y., Inoue A., Nakanomyo I., Ogawa M., Sawa S., Ohashi-Ito K., Matsubayashi Y. and Fukuda H.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 105 巻頁： 15208-15213 2008年

Identification of a biologically active, small, secreted peptide in Arabidopsis by in silico gene screening, followed by LC-MS-based structure analysis. 査読有り

Ohyama K, Ogawa M, Matsubayashi Y.

Plant J. 55 巻頁： 152-160 2008年

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

Peptidomics is a challenging field in which to create a link between genomic information and biological function through biochemical analysis of expressed peptides, including precise identification of post-translational modifications and proteolytic processing. We found that secreted peptides in Arabidopsis plants diffuse into the medium of whole-plant submerged cultures, and can be effectively identified by o-chlorophenol extraction followed by LC-MS analysis. Using this system, we first confirmed that a 12-amino-acid mature CLE44 peptide accumulated at a considerable level in the culture medium of transgenic plants overexpressing CLE44. Next, using an in silico approach, we identified a novel gene family encoding small secreted peptides that exhibit significant sequence similarity within the C-terminal short conserved domain. We determined that the mature peptide encoded by At1g47485, a member of this gene family, is a 15-amino-acid peptide containing two hydroxyproline residues derived from the conserved domain. This peptide, which we have named CEP1, is mainly expressed in the lateral root primordia and, when overexpressed or externally applied, significantly arrests root growth. CEP1 is a candidate for a novel peptide plant hormone.

　前のページ - 次のページ　

<松林 嘉克>

論文 - 松林 嘉克

<松林　嘉克>

論文 - 松林　嘉克