研究者詳細 - 小嶋　誠司

論文 - 小嶋　誠司

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超好熱菌Aquifex aeolicusの運動解析と大腸菌を用いたAquifexモーター固定子の機能解析

本間道夫, 小嶋誠司, 竹川宜宏, 西山雅祥, 金関剛史, 金井保

日本細菌学雑誌 70 巻 ( 1 ) 頁： 147 - 147 2015年2月

Interaction of the C-terminal tail of FliF with FliG from the Na+-driven flagellar motor of Vibrio alginolyticus. 査読有り

Ogawa R, Abe-Yoshizumi R, Kishi T, Homma M, Kojima S

Journal of bacteriology 197 巻 ( 1 ) 頁： 63 - 72 2015年1月

The MinD homolog FlhG regulates the synthesis of the single polar flagellum of Vibrio alginolyticus. 査読有り

Ono H, Takashima A, Hirata H, Homma M, Kojima S.

Mol. Microbiol. 98 巻頁： 130-141 2015年

FliL associates with the stator to support torque generation of the sodium-driven polar flagellar motor of Vibrio. 査読有り

Zhu S, Kumar A, Kojima S, Homma M.

Mol. Microbiol. 98 巻頁： 101-110 2015年

Interaction of the C-terminal tail of FliF with FliG from the Na+-driven flagellar motor of Vibrio alginolyticus 査読有り

Ogawa R, Abe-Yoshizumi R, Kishi T, Homma M, Kojima S.

J. Bacteriol. 197 巻頁： 63-72 2015年

Dynamism and regulation of the stator, the energy conversion complex of the bacterial flagellar motor. 招待有り査読有り

Seiji Kojima

Curr. Opin. Microbiol. 28 巻頁： 66-71 2015年

Nascent chain-monitored remodeling of the Sec machinery for salinity adaptation of marine bacteria. 査読有り

Ishii E, Chiba S, Hashimoto N, Kojima S, Homma M, Ito K, Akiyama Y, Mori H.

Proc. Natl. Acad. Sci. USA 112 巻頁： E5513-E5522 2015年

細菌べん毛モーター固定子の組込みと活性化機構

今田勝巳, 小嶋誠司

日本結晶学会誌 57 巻 ( 5 ) 頁： 291 - 296 2015年

記述言語：日本語出版者・発行元：The Crystallographic Society of Japan

Bacteria swim in aqueous environment by rotating the flagellum driven by a reversible motor embedded in the cell membrane. The torque of the motor is generated by the rotor-stator interaction coupled with the ion flow through the stator. A dozen of stators assemble around the rotor and are fixed to the peptidoglycan layer. The ion-conductivity of the stator is coupled to its assembly, and proper anchoring of the stator is required for torque generation. We determined the crystal structures of C-terminal fragments of the B-subunit of the stator including the region responsible for anchoring the stator, and performed the functional analyses using the mutants designed based on the structure. These studies revealed that the conformational change of the N-terminal region of the fragment is essential to form a functional stator around the rotor.

DOI： 10.5940/jcrsj.57.291

CiNii Books

その他リンク： https://jlc.jst.go.jp/DN/JLC/20016787177?from=CiNii

Conformational change in the periplamic region of the flagellar stator coupled with the assembly around the rotor. 査読有り

Zhu S, Takao M, Li N, Sakuma M, Nishino Y, Homma M, Kojima S, Imada K

Proceedings of the National Academy of Sciences of the United States of America 111 巻 ( 37 ) 頁： 13523 - 13528 2014年9月

Contribution of many charged residues at the stator-rotor interface of the Na+-driven flagellar motor to torque generation in Vibrio alginolyticus. 査読有り

Takekawa N, Kojima S, Homma M

Journal of bacteriology 196 巻 ( 7 ) 頁： 1377 - 1385 2014年4月

Construction of functional fragments of the cytoplasmic loop with the C-terminal region of PomA, a stator component of the Vibrio Na+ driven flagellar motor. 査読有り

Onoue Y, Abe-Yoshizumi R, Gohara M, Kobayashi S, Nishioka N, Kojima S, Homma M.

J. Biochem. 155 巻頁： 207-216 2014年3月

Construction of functional fragments of the cytoplasmic loop with the C-terminal region of PomA, a stator component of the Vibrio Na+ driven flagellar motor. 査読有り

Onoue Y, Abe-Yoshizumi R, Gohara M, Kobayashi S, Nishioka N, Kojima S, Homma M

Journal of biochemistry 155 巻 ( 3 ) 頁： 207 - 216 2014年3月

Biophysical characterization of the C-terminal region of FliG, an essential rotor component of the Na+-driven flagellar motor. 査読有り

Gohara M, Kobayashi S, Abe-Yoshizumi R, Nonoyama N, Kojima S, Asami Y, Homma M

Journal of biochemistry 155 巻 ( 2 ) 頁： 83 - 89 2014年2月

Structure, gene regulation and environmental response of flagella in Vibrio. 査読有り

Zhu S, Kojima S, Homma M

Frontiers in microbiology 4 巻 ( 410 ) 頁： 410 2013年12月

Mutation in the a-subunit of F(1)F(O)-ATPase causes an increased motility phenotype through the sodium-driven flagella of Vibrio. 査読有り

Terashima H, Terauchi T, Ihara K, Nishioka N, Kojima S, Homma M

Journal of biochemistry 154 巻 ( 2 ) 頁： 177 - 184 2013年8月

Fluorescence imaging of GFP-fused periplasmic components of Na+-driven flagellar motor using Tat pathway in Vibrio alginolyticus. 査読有り

Takekawa N, Kojima S, Homma M

Journal of biochemistry 153 巻 ( 6 ) 頁： 547 - 553 2013年6月

Na+ conductivity of the Na+-driven flagellar motor complex composed of unplugged wild-type or mutant PomB with PomA. 査読有り

Takekawa N, Terauchi T, Morimoto YV, Minamino T, Lo CJ, Kojima S, Homma M

Journal of biochemistry 153 巻 ( 5 ) 頁： 441 - 451 2013年5月

Insight into the assembly mechanism in the supramolecular rings of the sodium-driven Vibrio flagellar motor from the structure of FlgT. 査読有り

Terashima H, Li N, Sakuma M, Koike M, Kojima S, Homma M, Imada K

Proceedings of the National Academy of Sciences of the United States of America 110 巻 ( 15 ) 頁： 6133 - 6138 2013年4月

A novel dnaJ family gene, sflA, encodes an inhibitor of flagellation in marine Vibrio species. 査読有り

Kitaoka M, Nishigaki T, Ihara K, Nishioka N, Kojima S, Homma M

Journal of bacteriology 195 巻 ( 4 ) 頁： 816 - 822 2013年2月

Mutation in the a-subunit of F1FO-ATPase causes an increased motility phenotype through the sodium-driven flagella of Vibrio. 査読有り

Terashima H, Terauchi T, Ihara K, Nishioka N, Kojima S, Homma M.

J. Biochem. 154 巻頁： 177-184 2013年

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