研究者詳細 - 小嶋　誠司

論文 - 小嶋　誠司

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Structural analysis of S-ring composed of FliFG fusion proteins in marine Vibrio polar flagellar motor. 国際誌

Takekawa N, Nishikino T, Kishikawa J-i, Hirose M, Kinoshita M, Kojima S, Minamino T, Uchihashi T, Kato T, Imada K, Homma M

mBio 15 巻 ( 10 ) 頁： e0126124 2024年9月

Regulatory Role of a Hydrophobic Core in the FliG C-Terminal Domain in the Rotary Direction of a Flagellar Motor.

Nishikino T, Hatano A, Kojima S, Homma M

Biomolecules 15 巻 ( 2 ) 2025年2月

Structural insight into sodium ion pathway in the bacterial flagellar stator from marine Vibrio.

Nishikino T, Takekawa N, Kishikawa JI, Hirose M, Kojima S, Homma M, Kato T, Imada K

Proceedings of the National Academy of Sciences of the United States of America 122 巻 ( 1 ) 頁： e2415713122 2025年1月

Structure, function, and biophysics of bacterial motility and the flagellar motor-IUPAB2024 session commentary.

Baker MAB, Kojima S

Biophysical reviews 16 巻 ( 5 ) 頁： 537 - 538 2024年10月

Deciphering the genomes of motility-deficient mutants of Vibrio alginolyticus 138-2.

Uesaka K, Inaba K, Nishioka N, Kojima S, Homma M, Ihara K

PeerJ 12 巻頁： e17126 2024年

Interaction of FlhF, SRP-like GTPase with FliF, MS ring component assembling the initial structure of flagella in marine Vibrio.

Fukushima Y, Homma M, Kojima S

Journal of biochemistry 174 巻 ( 2 ) 頁： 125 - 130 2023年7月

Purification of the Na(+)-Driven PomAB Stator Complex and Its Analysis Using ATR-FTIR Spectroscopy.

Kojima S, Homma M, Kandori H

Methods in molecular biology (Clifton, N.J.) 2646 巻頁： 95 - 107 2023年

1. Function and structure of FlaK, a master regulator of the polar flagellar genes in marine Vibrio. 査読有り

Homma M, Kobayakawa T, Hao Y, Nishikino T, Kojima S

Journal of Bacteriology 204 巻 ( 11 ) 頁： e0032022 2022年11月

Roles of the second messenger c-di-GMP in bacteria: Focusing on the topics of flagellar regulation and Vibrio spp.

Homma M, Kojima S

Genes to cells : devoted to molecular & cellular mechanisms 27 巻 ( 3 ) 頁： 157 - 172 2022年3月

The Periplasmic Domain of the Ion-Conducting Stator of Bacterial Flagella Regulates Force Generation.

Homma M, Kojima S

Frontiers in microbiology 13 巻頁： 869187 2022年

Editorial: Flagellar Motors and Force Sensing in Bacteria.

Baker MAB, Kojima S, Nord AL, Partridge JD

Frontiers in microbiology 13 巻頁： 833011 2022年

Solution structure analysis of the periplasmic region of bacterial flagellar motor stators by small angle X-ray scattering.

Liew CW, Hynson RM, Ganuelas LA, Shah-Mohammadi N, Duff AP, Kojima S, Homma M, Lee LK

Biochemical and biophysical research communications 495 巻 ( 2 ) 頁： 1614 - 1619 2018年1月

Probing the mechanism of bacterial flagella assembly by real-time fluorescent imaging

Zhuang X. -Y., Zhao Z., Homma M., Kojima S., Bai F., Lo C. -J.

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS 46 巻頁： S297 - S297 2017年7月

Sodium-Driven Energy Conversion for Flagellar Rotation of the Earliest Divergent Hyperthermophilic Bacterium

Takekawa Norihiko, Nishiyama Masayoshi, Kaneseki Tsuyoshi, Kanai Tamotsu, Atomi Haruyuki, Kojima Seij, Homma Michio

BIOPHYSICAL JOURNAL 110 巻 ( 3 ) 頁： 468A - 468A 2016年2月

Pressure-Speed Relationship of the Sodium-Driven Flagellar Motor of Vibrio Alginolyticus

Nishiyama Masayoshi, Shimoda Yoshiki, Kimura Yoshifumi, Terazima Masahide, Homma Michio, Kojima Seiji

BIOPHYSICAL JOURNAL 106 巻 ( 2 ) 頁： 578A - 578A 2014年1月

Structure and function of the peptidoglycan-binding (PGB) domain of bacterial flagellar motor component MotB using systematic mutagenesis and chimeric protein with Pal

Hizukuri, Y; Morton, JF; Yakushi, T; Kojima, S; Homma, M

GENES & GENETIC SYSTEMS 83 巻 ( 6 ) 頁： 488 - 488 2008年12月

The polar flagellar motor of Vibrio cholerae is driven by an Na+ motive force

Kojima S, Yamamoto K, Kawagishi I, Homma M

JOURNAL OF BACTERIOLOGY 181 巻 ( 6 ) 頁： 1927 - 1930 1999年3月

Structural insight into sodium ion pathway in the bacterial flagellar stator from marineVibrio

Tatsuro Nishikino, Norihiro Takekawa, Jun-ichi Kishikawa, Mika Hirose, Seiji Kojima, Michio Homma, Takayuki Kato, Katsumi Imada

2024年7月

出版者・発行元：Cold Spring Harbor Laboratory

Abstract

Many bacteria swim in liquid or swarm on surface using the flagellum rotated by a motor driven by specific ion flow. The motor consists of the rotor and stator, and the stator converts the energy of ion flow to mechanical rotation. However, the ion pathway and the mechanism of stator rotation coupled with specific ion flow are still obscure. Here, we determined the structures of the Na+-driven stator ofVibrio, namely PomAB, in the presence and absence of sodium ions and the structure with its specific inhibitor, phenamil, by cryo-electron microscopy. The structures and following functional analysis revealed the sodium ion pathway, the mechanism of ion selectivity, and the inhibition mechanism by phenamil. We propose a model of sodium ion flow coupled with the stator rotation based on the structures. This work provides insights into the molecular mechanisms of ion specificity and conversion of the electrochemical potential into mechanical functions.

DOI： 10.1101/2024.07.15.603494

Roles of linker region flanked by transmembrane and peptidoglycan binding region of PomB in energy conversion of the Vibrio flagellar motor. 国際誌

Miyamura Y, Nishikino T, Koiwa H, Homma M, Kojima S

Genes to cells : devoted to molecular & cellular mechanisms 29 巻 ( 4 ) 頁： 282 - 289 2024年2月

Changes in the hydrophobic network of the FliG(MC) domain induce rotational switching of the flagellar motor. 国際誌

Nishikino T, Hijikata A, Kojima S, Shirai T, Kainosho M, Homma M, Miyanoiri Y

iScience 26 巻 ( 8 ) 頁： 107320 - 107320 2023年8月

Site-Directed Cross-Linking Between Bacterial Flagellar Motor Proteins In Vivo.

Terashima H, Homma M, Kojima S

Methods in molecular biology (Clifton, N.J.) 2646 巻頁： 71 - 82 2023年2月

Ring formation by Vibrio fusion protein composed of FliF and FliG, MS-ring and C-ring component of bacterial flagellar motor in membrane.

Takahashi K, Nishikino T, Kajino H, Kojima S, Uchihashi T, Homma M

Biophysics and physicobiology 20 巻 ( 2 ) 頁： e200028 2023年

Mutations in the stator protein PomA affect switching of rotational direction in bacterial flagellar motor.

Terashima H, Hori K, Ihara K, Homma M, Kojima S

Scientific reports 12 巻 ( 1 ) 頁： 2979 2022年12月

Structure of MotA, a flagellar stator protein, from hyperthermophile.

Nishikino T, Takekawa N, Tran DP, Kishikawa JI, Hirose M, Onoe S, Kojima S, Homma M, Kitao A, Kato T, Imada K

Biochemical and biophysical research communications 631 巻頁： 78 - 85 2022年11月

Function and Structure of FlaK, a Master Regulator of the Polar Flagellar Genes in Marine Vibrio 国際誌

Homma Michio, Kobayakawa Tomoya, Hao Yuxi, Nishikino Tatsuro, Kojima Seiji

JOURNAL OF BACTERIOLOGY 204 巻 ( 11 ) 頁： e0032022 2022年10月

Formation of multiple flagella caused by a mutation of the flagellar rotor protein FliM in Vibrio alginolyticus. 国際誌

Homma M, Takekawa N, Fujiwara K, Hao Y, Onoue Y, Kojima S

Genes to cells : devoted to molecular & cellular mechanisms 27 巻 ( 9 ) 頁： 568 - 578 2022年9月

Functional analysis of the N-terminal region of Vibrio FlhG, a MinD-type ATPase in flagellar number control. 査読有り

Homma M, Mizuno A, Hao Y, Kojima S

Journal of biochemistry 172 巻 ( 2 ) 頁： 99 - 107 2022年6月

The Periplasmic Domain of the Ion-Conducting Stator of Bacterial Flagella Regulates Force Generation 査読有り

Homma M, Kojima S

2022年4月

Hoop-like role of the cytosolic interface helix in Vibrio PomA, an ion-conducting membrane protein, in the bacterial flagellar motor. 国際誌

Nishikino T, Sagara Y, Terashima H, Homma M, Kojima S

Journal of biochemistry 171 巻 ( 4 ) 頁： 443 - 450 2022年1月

Achievements in bacterial flagellar research with focus on Vibrio species.

Homma M, Nishikino T, Kojima S

Microbiology and immunology 66 巻 ( 2 ) 頁： 75 - 95 2022年1月

ZomB is essential for chemotaxis of Vibrio alginolyticus by the rotational direction control of the polar flagellar motor. 国際誌

Takekawa N, Nishikino T, Hori K, Kojima S, Imada K, Homma M

Genes to cells : devoted to molecular & cellular mechanisms 26 巻 ( 11 ) 頁： 927 - 937 2021年9月

A slight bending of an α-helix in FliM creates a counterclockwise-locked structure of the flagellar motor in Vibrio. 国際誌

Takekawa N, Nishikino T, Yamashita T, Hori K, Onoue Y, Ihara K, Kojima S, Homma M, Imada K

Journal of biochemistry 170 巻 ( 4 ) 頁： 531 - 538 2021年6月

Putative Spanner Function of the Vibrio PomB Plug Region in the Stator Rotation Model for Flagellar Motor

Homma Michio, Terashima Hiroyuki, Koiwa Hiroaki, Kojima Seiji

JOURNAL OF BACTERIOLOGY 203 巻 ( 16 ) 2021年6月

Two Distinct Conformations in 34 FliF Subunits Generate Three Different Symmetries within the Flagellar MS-Ring. 招待有り査読有り

mBio. 2021年3月

Two Distinct Conformations in 34 FliF Subunits Generate Three Different Symmetries within the Flagellar MS-Ring.

Takekawa N, Kawamoto A, Sakuma M, Kato T, Kojima S, Kinoshita M, Minamino T, Namba K, Homma M, Imada K

mBio 12 巻 ( 2 ) 2021年3月

Role of the N- and C-terminal regions of FliF, the MS ring component in Vibrio flagellar basal body. 国際誌

Kojima S, Kajino H, Hirano K, Inoue Y, Terashima H, Homma M

Journal of bacteriology 203 巻 ( 9 ) 2021年2月

Site-directed crosslinking identifies the stator-rotor interaction surfaces in a hybrid bacterial flagellar motor. 国際誌

Terashima H, Kojima S, Homma M

Journal of bacteriology 203 巻 ( 9 ) 2021年2月

Stator Dynamics Depending on Sodium Concentration in Sodium-Driven Bacterial Flagellar Motors. 国際誌

Lin TS, Kojima S, Fukuoka H, Ishijima A, Homma M, Lo CJ

Frontiers in microbiology 12 巻頁： 765739 - 765739 2021年

The flagellar motor of Vibrio alginolyticus undergoes major structural remodeling during rotational switching. 国際誌

Carroll BL, Nishikino T, Guo W, Zhu S, Kojima S, Homma M, Liu J

eLife 9 巻 2020年9月

Live-cell fluorescence imaging reveals dynamic production and loss of bacterial flagella. 国際誌

Zhuang XY, Guo S, Li Z, Zhao Z, Kojima S, Homma M, Wang P, Lo CJ, Bai F

Molecular microbiology 114 巻 ( 2 ) 頁： 279 - 291 2020年8月

Assembly mechanism of a supramolecular MS-ring complex to initiate bacterial flagellar biogenesis in Vibrio species. 査読有り国際誌

Terashima H, Hirano K, Inoue Y, Tokano T, Kawamoto A, Kato T, Yamaguchi E, Namba K, Uchihashi T, Kojima S, Homma M

Journal of bacteriology 202 巻 ( 16 ) 2020年6月

Regulation of the Single Polar Flagellar Biogenesis. 国際誌

Kojima S, Terashima H, Homma M

Biomolecules 10 巻 ( 4 ) 2020年4月

Characterization of PomA periplasmic loop and sodium ion entering in stator complex of sodium-driven flagellar motor 国際誌

Nishikino Tatsuro, Iwatsuki Hiroto, Mino Taira, Kojima Seiji, Homma Michio

JOURNAL OF BIOCHEMISTRY 167 巻 ( 4 ) 頁： 389 - 398 2020年4月

Characterization of the MinD/ParA-type ATPase FlhG in Vibrio alginolyticus and implications for function of its monomeric form. 国際誌

Kojima S, Imura Y, Hirata H, Homma M

Genes to cells : devoted to molecular & cellular mechanisms 25 巻 ( 4 ) 頁： 279 - 287 2020年4月

Characterization of FliL Proteins in Bradyrhizobium diazoefficiens: Lateral FliL Supports Swimming Motility, and Subpolar FliL Modulates the Lateral Flagellar System. 国際誌

Mengucci F, Dardis C, Mongiardini EJ, Althabegoiti MJ, Partridge JD, Kojima S, Homma M, Quelas JI, Lodeiro AR

Journal of bacteriology 202 巻 ( 5 ) 2020年2月

In Situ Structure of the Vibrio Polar Flagellum Reveals a Distinct Outer Membrane Complex and Its Specific Interaction with the Stator. 査読有り国際誌

Zhu S, Nishikino T, Takekawa N, Terashima H, Kojima S, Imada K, Homma M, Liu J

Journal of bacteriology 202 巻 ( 4 ) 2020年1月

Tree of motility - A proposed history of motility systems in the tree of life. 査読有り国際誌

Miyata M, Robinson RC, Uyeda TQP, Fukumori Y, Fukushima SI, Haruta S, Homma M, Inaba K, Ito M, Kaito C, Kato K, Kenri T, Kinosita Y, Kojima S, Minamino T, Mori H, Nakamura S, Nakane D, Nakayama K, Nishiyama M, Shibata S, Shimabukuro K, Tamakoshi M, Taoka A, Tashiro Y, Tulum I, Wada H, Wakabayashi KI

Genes to cells : devoted to molecular & cellular mechanisms 25 巻 ( 1 ) 頁： 6 - 21 2020年1月

[Flagellar related genes and functions in Vibrio].

Nishikino T, Kojima S, Homma M

Nihon saikingaku zasshi. Japanese journal of bacteriology 75 巻 ( 3 ) 頁： 195 - 214 2020年

Structure of the periplasmic domain of SflA involved in spatial regulation of the flagellar biogenesis of Vibrio reveals a TPR/SLR-like fold. 査読有り国際誌

Sakuma M, Nishikawa S, Inaba S, Nishigaki T, Kojima S, Homma M, Imada K

Journal of biochemistry 166 巻 ( 2 ) 頁： 197 - 204 2019年8月

Effect of PlzD, a YcgR homologue of c-di-GMP-binding protein, on polar flagellar motility in Vibrio alginolyticus. 査読有り国際誌

Kojima S, Yoneda T, Morimoto W, Homma M

Journal of biochemistry 166 巻 ( 1 ) 頁： 77 - 88 2019年7月

Effect of sodium ions on conformations of the cytoplasmic loop of the PomA stator protein of Vibrio alginolyticus. 査読有り

Mino T, Nishikino T, Iwatsuki H, Kojima S, Homma M

J. Biochem. 166 巻 ( 4 ) 頁： 331 - 341 2019年5月

Structure of Vibrio FliL, a New Stomatin-like Protein That Assists the Bacterial Flagellar Motor Function. 査読有り国際誌

Takekawa N, Isumi M, Terashima H, Zhu S, Nishino Y, Sakuma M, Kojima S, Homma M, Imada K

mBio 10 巻 ( 2 ) 頁： e00292 2019年3月

Rotational direction of flagellar motor from the conformation of FliG middle domain in marine Vibrio. 査読有り国際誌

Nishikino T, Hijikata A, Miyanoiri Y, Onoue Y, Kojima S, Shirai T, Homma M

Scientific reports 8 巻 ( 1 ) 頁： 17793 - 17793 2018年12月

The Vibrio H-Ring Facilitates the Outer Membrane Penetration of the Polar Sheathed Flagellum. 査読有り国際誌

Zhu S, Nishikino T, Kojima S, Homma M, Liu J

Journal of bacteriology 200 巻 ( 21 ) 2018年11月

Autonomous control mechanism of stator assembly in the bacterial flagellar motor in response to changes in the environment. 査読有り国際誌

Minamino T, Terahara N, Kojima S, Namba K

Molecular microbiology 109 巻 ( 6 ) 頁： 723 - 734 2018年9月

Biochemical analysis of GTPase FlhF which controls the number and position of flagellar formation in marine Vibrio. 査読有り国際誌

Kondo S, Imura Y, Mizuno A, Homma M, Kojima S

Scientific reports 8 巻 ( 1 ) 頁： 12115 - 12115 2018年8月

The role of conserved charged residues in the bidirectional rotation of the bacterial flagellar motor. 査読有り国際誌

Onoue Y, Takekawa N, Nishikino T, Kojima S, Homma M

MicrobiologyOpen 7 巻 ( 4 ) 頁： e00587 2018年8月

FliL association with flagellar stator in the sodium-driven Vibrio motor characterized by the fluorescent microscopy. 査読有り国際誌

Lin TS, Zhu S, Kojima S, Homma M, Lo CJ

Scientific reports 8 巻 ( 1 ) 頁： 11172 - 11172 2018年7月

The Helix Rearrangement in the Periplasmic Domain of the Flagellar Stator B Subunit Activates Peptidoglycan Binding and Ion Influx. 査読有り

Kojima S, Takao M, Almira G, Kawahara I, Sakuma M, Homma M, Kojima C, Imada K

Structure (London, England : 1993) 26 巻 ( 4 ) 頁： 590 - 598.e5 2018年4月

Structural and Functional Analysis of the C-Terminal Region of FliG, an Essential Motor Component of Vibrio Na(+)-Driven Flagella. 査読有り

Miyanoiri Y, Hijikata A, Nishino Y, Gohara M, Onoue Y, Kojima S, Kojima C, Shirai T, Kainosho M, Homma M

Structure (London, England : 1993) 25 巻 ( 10 ) 頁： 1540 - + 2017年10月

Molecular architecture of the sheathed polar flagellum in Vibrio alginolyticus. 査読有り

Zhu S, Nishikino T, Hu B, Kojima S, Homma M, Liu J

Proceedings of the National Academy of Sciences of the United States of America 114 巻 ( 41 ) 頁： 10966 - 10971 2017年10月

Localization and domain characterization of the SflA regulator of flagellar formation in Vibrio alginolyticus. 査読有り

Inaba S, Nishigaki T, Takekawa N, Kojima S, Homma M

Genes to cells : devoted to molecular & cellular mechanisms 22 巻 ( 7 ) 頁： 619 - 627 2017年7月

Biochemical characterization of the flagellar stator-associated inner membrane protein FliL from Vibrio alginolyticus. 査読有り

Kumar A, Isumi M, Sakuma M, Zhu S, Nishino Y, Onoue Y, Kojima S, Miyanoiri Y, Imada K, Homma M

Journal of biochemistry 161 巻 ( 4 ) 頁： 331 - 337 2017年4月

Mutational analysis and overproduction effects of MotX, an essential component for motor function of Na+-driven polar flagella of Vibrio. 国際誌

Takekawa N, Kojima S, Homma M

Journal of biochemistry 161 巻 ( 2 ) 頁： 159 - 166 2017年2月

Structural and functional analysis of the C-terminal region of FliG, an essential motor component of Vibrio Na+-driven flagella. 査読有り

Miyanoiri Y, Hijikata A, Nishino Y, Gohara M, Onoue Y, Kojima S, Kojima C, Shirai T, Kainosho M, Homma M.

Structure 25 巻頁： 1-9 2017年

Localization and domain characterization of the SflA regulator of flagellar formation in Vibrio alginolyticus. 査読有り

Inaba S, Nishigaki T, Takekawa N, Kojima S, Homma M.

Genes Cells 7 巻頁： 619-627 2017年

Mechanism of Stator Assembly and Incorporation into the Flagellar Motor. 査読有り

Kojima S

Methods in molecular biology (Clifton, N.J.) 1593 巻頁： 147 - 159 2017年

Analysis of the GTPase motif of FlhF in the control of the number and location of polar flagella in Vibrio alginolyticus. 査読有り

Kondo S, Homma M, Kojima S

Biophysics and physicobiology 14 巻頁： 173 - 181 2017年

HubP, a Polar Landmark Protein, Regulates Flagellar Number by Assisting in the Proper Polar Localization of FlhG in Vibrio alginolyticus. 査読有り

Takekawa N, Kwon S, Nishioka N, Kojima S, Homma M

Journal of bacteriology 198 巻 ( 22 ) 頁： 3091 - 3098 2016年11月

The tetrameric MotA complex as the core of the flagellar motor stator from hyperthermophilic bacterium. 査読有り

Takekawa N, Terahara N, Kato T, Gohara M, Mayanagi K, Hijikata A, Onoue Y, Kojima S, Shirai T, Namba K, Homma M

Scientific reports 6 巻頁： 31526 2016年8月

FliH and FliI ensure efficient energy coupling of flagellar type III protein export in Salmonella. 査読有り

Minamino T, Kinoshita M, Inoue Y, Morimoto YV, Ihara K, Koya S, Hara N, Nishioka N, Kojima S, Homma M, Namba K

MicrobiologyOpen 5 巻 ( 3 ) 頁： 424 - 435 2016年6月

Serine suppresses the motor function of a periplasmic PomB mutation in the Vibrio flagella stator. 査読有り

Nishikino T, Zhu S, Takekawa N, Kojima S, Onoue Y, Homma M

Genes to cells : devoted to molecular & cellular mechanisms 21 巻 ( 5 ) 頁： 505 - 516 2016年5月

細菌べん毛の回転および本数制御機構に関する研究招待有り

小嶋誠司

日本細菌学雑誌 71 巻 ( 3 ) 頁： 185-197 2016年

[Studies on the mechanism of bacterial flagellar rotation and the flagellar number regulation]. 査読有り

Kojima S

Nihon saikingaku zasshi. Japanese journal of bacteriology 71 巻 ( 3 ) 頁： 185 - 97 2016年

Effect of FliG three amino acids deletion in Vibrio polar-flagellar rotation and formation. 査読有り

Onoue Y, Kojima S, Homma M

Journal of biochemistry 158 巻 ( 6 ) 頁： 523 - 529 2015年12月

Dynamism and regulation of the stator, the energy conversion complex of the bacterial flagellar motor. 査読有り

Kojima S

Current opinion in microbiology 28 巻頁： 66 - 71 2015年12月

FliL associates with the stator to support torque generation of the sodium-driven polar flagellar motor of Vibrio. 査読有り

Zhu S, Kumar A, Kojima S, Homma M

Molecular microbiology 98 巻 ( 1 ) 頁： 101 - 110 2015年10月

The MinD homolog FlhG regulates the synthesis of the single polar flagellum of Vibrio alginolyticus. 査読有り

Ono H, Takashima A, Hirata H, Homma M, Kojima S

Molecular microbiology 98 巻 ( 1 ) 頁： 130 - 141 2015年10月

Sodium-driven energy conversion for flagellar rotation of the earliest divergent hyperthermophilic bacterium. 査読有り

Takekawa N, Nishiyama M, Kaneseki T, Kanai T, Atomi H, Kojima S, Homma M

Scientific reports 5 巻頁： 12711 2015年8月

Functional chimeras of flagellar stator proteins between E. coli MotB and Vibrio PomB at the periplasmic region in Vibrio or E. coli. 査読有り

Nishino Y, Onoue Y, Kojima S, Homma M

MicrobiologyOpen 4 巻 ( 2 ) 頁： 323 - 331 2015年4月

超好熱菌Aquifex aeolicusの運動解析と大腸菌を用いたAquifexモーター固定子の機能解析

本間道夫, 小嶋誠司, 竹川宜宏, 西山雅祥, 金関剛史, 金井保

日本細菌学雑誌 70 巻 ( 1 ) 頁： 147 - 147 2015年2月

Interaction of the C-terminal tail of FliF with FliG from the Na+-driven flagellar motor of Vibrio alginolyticus. 査読有り

Ogawa R, Abe-Yoshizumi R, Kishi T, Homma M, Kojima S

Journal of bacteriology 197 巻 ( 1 ) 頁： 63 - 72 2015年1月

The MinD homolog FlhG regulates the synthesis of the single polar flagellum of Vibrio alginolyticus. 査読有り

Ono H, Takashima A, Hirata H, Homma M, Kojima S.

Mol. Microbiol. 98 巻頁： 130-141 2015年

FliL associates with the stator to support torque generation of the sodium-driven polar flagellar motor of Vibrio. 査読有り

Zhu S, Kumar A, Kojima S, Homma M.

Mol. Microbiol. 98 巻頁： 101-110 2015年

Interaction of the C-terminal tail of FliF with FliG from the Na+-driven flagellar motor of Vibrio alginolyticus 査読有り

Ogawa R, Abe-Yoshizumi R, Kishi T, Homma M, Kojima S.

J. Bacteriol. 197 巻頁： 63-72 2015年

Dynamism and regulation of the stator, the energy conversion complex of the bacterial flagellar motor. 招待有り査読有り

Seiji Kojima

Curr. Opin. Microbiol. 28 巻頁： 66-71 2015年

Nascent chain-monitored remodeling of the Sec machinery for salinity adaptation of marine bacteria. 査読有り

Ishii E, Chiba S, Hashimoto N, Kojima S, Homma M, Ito K, Akiyama Y, Mori H.

Proc. Natl. Acad. Sci. USA 112 巻頁： E5513-E5522 2015年

細菌べん毛モーター固定子の組込みと活性化機構

今田勝巳, 小嶋誠司

日本結晶学会誌 57 巻 ( 5 ) 頁： 291 - 296 2015年

記述言語：日本語出版者・発行元：The Crystallographic Society of Japan

Bacteria swim in aqueous environment by rotating the flagellum driven by a reversible motor embedded in the cell membrane. The torque of the motor is generated by the rotor-stator interaction coupled with the ion flow through the stator. A dozen of stators assemble around the rotor and are fixed to the peptidoglycan layer. The ion-conductivity of the stator is coupled to its assembly, and proper anchoring of the stator is required for torque generation. We determined the crystal structures of C-terminal fragments of the B-subunit of the stator including the region responsible for anchoring the stator, and performed the functional analyses using the mutants designed based on the structure. These studies revealed that the conformational change of the N-terminal region of the fragment is essential to form a functional stator around the rotor.

DOI： 10.5940/jcrsj.57.291

CiNii Books

その他リンク： https://jlc.jst.go.jp/DN/JLC/20016787177?from=CiNii

Conformational change in the periplamic region of the flagellar stator coupled with the assembly around the rotor. 査読有り

Zhu S, Takao M, Li N, Sakuma M, Nishino Y, Homma M, Kojima S, Imada K

Proceedings of the National Academy of Sciences of the United States of America 111 巻 ( 37 ) 頁： 13523 - 13528 2014年9月

Contribution of many charged residues at the stator-rotor interface of the Na+-driven flagellar motor to torque generation in Vibrio alginolyticus. 査読有り

Takekawa N, Kojima S, Homma M

Journal of bacteriology 196 巻 ( 7 ) 頁： 1377 - 1385 2014年4月

Construction of functional fragments of the cytoplasmic loop with the C-terminal region of PomA, a stator component of the Vibrio Na+ driven flagellar motor. 査読有り

Onoue Y, Abe-Yoshizumi R, Gohara M, Kobayashi S, Nishioka N, Kojima S, Homma M.

J. Biochem. 155 巻頁： 207-216 2014年3月

Construction of functional fragments of the cytoplasmic loop with the C-terminal region of PomA, a stator component of the Vibrio Na+ driven flagellar motor. 査読有り

Onoue Y, Abe-Yoshizumi R, Gohara M, Kobayashi S, Nishioka N, Kojima S, Homma M

Journal of biochemistry 155 巻 ( 3 ) 頁： 207 - 216 2014年3月

Biophysical characterization of the C-terminal region of FliG, an essential rotor component of the Na+-driven flagellar motor. 査読有り

Gohara M, Kobayashi S, Abe-Yoshizumi R, Nonoyama N, Kojima S, Asami Y, Homma M

Journal of biochemistry 155 巻 ( 2 ) 頁： 83 - 89 2014年2月

Structure, gene regulation and environmental response of flagella in Vibrio. 査読有り

Zhu S, Kojima S, Homma M

Frontiers in microbiology 4 巻 ( 410 ) 頁： 410 2013年12月

Mutation in the a-subunit of F(1)F(O)-ATPase causes an increased motility phenotype through the sodium-driven flagella of Vibrio. 査読有り

Terashima H, Terauchi T, Ihara K, Nishioka N, Kojima S, Homma M

Journal of biochemistry 154 巻 ( 2 ) 頁： 177 - 184 2013年8月

Fluorescence imaging of GFP-fused periplasmic components of Na+-driven flagellar motor using Tat pathway in Vibrio alginolyticus. 査読有り

Takekawa N, Kojima S, Homma M

Journal of biochemistry 153 巻 ( 6 ) 頁： 547 - 553 2013年6月

Na+ conductivity of the Na+-driven flagellar motor complex composed of unplugged wild-type or mutant PomB with PomA. 査読有り

Takekawa N, Terauchi T, Morimoto YV, Minamino T, Lo CJ, Kojima S, Homma M

Journal of biochemistry 153 巻 ( 5 ) 頁： 441 - 451 2013年5月

Insight into the assembly mechanism in the supramolecular rings of the sodium-driven Vibrio flagellar motor from the structure of FlgT. 査読有り

Terashima H, Li N, Sakuma M, Koike M, Kojima S, Homma M, Imada K

Proceedings of the National Academy of Sciences of the United States of America 110 巻 ( 15 ) 頁： 6133 - 6138 2013年4月

A novel dnaJ family gene, sflA, encodes an inhibitor of flagellation in marine Vibrio species. 査読有り

Kitaoka M, Nishigaki T, Ihara K, Nishioka N, Kojima S, Homma M

Journal of bacteriology 195 巻 ( 4 ) 頁： 816 - 822 2013年2月

Mutation in the a-subunit of F1FO-ATPase causes an increased motility phenotype through the sodium-driven flagella of Vibrio. 査読有り

Terashima H, Terauchi T, Ihara K, Nishioka N, Kojima S, Homma M.

J. Biochem. 154 巻頁： 177-184 2013年

Insight into the assembly mechanism in the supramolecular rings of the sodium-driven Vibrio flagellar motor from the structure of FlgT. 査読有り

Terashima H, Li N, Sakuma M, Koike M, Kojima S, Homma M, Imada K.

Proc. Natl. Acad. Sci. U. S. A. 110 巻頁： 6133-6138 2013年

Fluorescence imaging of GFP-fused periplasmic components of Na+-driven flagellar motor using Tat pathway in Vibrio alginolyticus. 査読有り

Takekawa N, Kojima S, Homma M.

J. Biochem. 153 巻頁： 547-553 2013年

Expression, purification and biochemical characterization of the cytoplasmic loop of PomA, a stator component of the Na+ driven flagellar motor. 査読有り

Abe-Yoshizumi R, Kobayashi S, Gohara M, Hayashi K, Kojima C, Kojima S, Sudo Y, Asami Y, Homma M.

Biophysics 9 巻頁： 21-29 2013年

Na+ conductivity of the Na+-driven flagellar motor complex composed of unplugged wild-type or mutant PomB with PomA. 査読有り

Takekawa N, Terauchi T, Morimoto YV, Minamino T, Lo CJ, Kojima S, Homma M.

J. Biochem. 153 巻頁： 441-451 2013年

A novel dnaJ family gene, sflA, encodes an inhibitor of flagellation in marine Vibrio species. 査読有り

Kitaoka M, Nishigaki T, Ihara K, Nishioka N, Kojima S, Homma M.

J. Bacteriol. 195 巻頁： 816-822 2013年

Expression, purification and biochemical characterization of the cytoplasmic loop of PomA, a stator component of the Na(+) driven flagellar motor. 査読有り

Abe-Yoshizumi R, Kobayashi S, Gohara M, Hayashi K, Kojima C, Kojima S, Sudo Y, Asami Y, Homma M

Biophysics (Nagoya-shi, Japan) 9 巻頁： 21 - 29 2013年

Intragenic suppressor of a plug deletion nonmotility mutation in PotB, a chimeric stator protein of sodium-driven flagella. 査読有り

Zhu S, Homma M, Kojima S

Journal of bacteriology 194 巻 ( 24 ) 頁： 6728 - 6735 2012年12月

Bacterial motility measured by a miniature chamber for high-pressure microscopy. 査読有り

Nishiyama M, Kojima S

International journal of molecular sciences 13 巻 ( 7 ) 頁： 9225 - 9239 2012年7月

Characterization of PomA Mutants Defective in the Functional Assembly of the Na+-Driven Flagellar Motor in Vibrio alginolyticus 査読有り

Takekawa, N; Li, N; Kojima, S; Homma, M

JOURNAL OF BACTERIOLOGY 194 巻 ( 8 ) 頁： 1934 - 1939 2012年4月

Instantaneous and Quantitative Single Cells Viability Determination Using Dual Nanoprobe Inside ESEM 査読有り

Ahmad, MR; Nakajima, M; Kojima, M; Kojima, S; Homma, M; Fukuda, T

IEEE TRANSACTIONS ON NANOTECHNOLOGY 11 巻 ( 2 ) 頁： 298 - 306 2012年3月

Nanofork for Single Cells Adhesion Measurement via ESEM-Nanomanipulator System 査読有り

Ahmad, MR; Nakajima, M; Kojima, M; Kojima, S; Homma, M; Fukuda, T

IEEE TRANSACTIONS ON NANOBIOSCIENCE 11 巻 ( 1 ) 頁： 70 - 78 2012年3月

ペリプラズム側構造から見たべん毛モーター構築とモーターの活性化機構査読有り

小嶋誠司、今田勝巳

生物物理 52 巻 ( 1 ) 頁： 18-21 2012年2月

ペリプラズム側構造から見たべん毛モーター構築とモーターの活性化機構

小嶋誠司, 今田勝巳

生物物理 52 巻 ( 1 ) 頁： 18 - 21 2012年1月

記述言語：日本語出版者・発行元：The Biophysical Society of Japan General Incorporated Association

Bacterial flagellar motor is an ion-driven supramolecular nanomachine embedded in the cell envelope. Rotor-stator interaction that couples to the specific ion translocation through the stator channel is the nature of torque generation. To produce fully functional motor, multiple stator complexes must be incorporated around the rotor at appropriate places. However, such stator assembly mechanism has not been investigated by the structural point of view. Here we describe stator assembly and activation mechanism revealed from the crystal structure of a motor component located in the periplasmic space, suggesting the dynamic conformational changes in the stator during its assembly-coupled activation.

DOI： 10.2142/biophys.52.018

CiNii Books

その他リンク： https://jlc.jst.go.jp/DN/JALC/00387646297?from=CiNii

Characterization of PomA mutants defective in the functional assembly of the Na+-driven flagellar motor in Vibrio alginolyticus 査読有り

Takekawa N, Li N, Kojima S, Homma M

Journal of Bacteriology 194 巻頁： 1934-1939 2012年

Nanofork for single cells adhesion measurement via ESEM-nanomanipulator system 査読有り

Ahmad MR, Nakajima M, Kojima M, Kojima S, Homma M, Fukuda T

IEEE Trans Nanobioscience 11 巻頁： 70-78 2012年

Intragenic suppressor of a plug deletion nonmotility mutation in PotB, a chimeric stator protein of sodium-driven flagella. 査読有り

Zhu S, Homma M, Kojima S

J. Bacteriol. 194 巻頁： 6728-6735 2012年

Bacterial motility measured by a miniature chamber for high-pressure microscopy. 査読有り

Nishiyama M and Kojima S

Int. J. Mol. Sci. 13 巻頁： 9225-9239 2012年

Evaluation of the Single Yeast Cell's Adhesion to ITO Substrates With Various Surface Energies via ESEM Nanorobotic Manipulation System. 査読有り

Shen Y, Ahmad MR, Nakajima M, Kojima S, Homma M, Fukuda T.

IEEE Trans Nanobioscience 10 巻 ( 4 ) 頁： 217-224 2011年12月

Evaluation of the Single Yeast Cell's Adhesion to ITO Substrates With Various Surface Energies via ESEM Nanorobotic Manipulation System 査読有り

Shen, YJ; Ahmad, MR; Nakajima, M; Kojima, S; Homma, M; Fukuda, T

IEEE TRANSACTIONS ON NANOBIOSCIENCE 10 巻 ( 4 ) 頁： 217 - 224 2011年12月

Mutations targeting the C-terminal domain of FliG can disrupt motor assembly in the Na(+)-driven flagella of Vibrio alginolyticus. 査読有り

Kojima S, Nonoyama N, Takekawa N, Fukuoka H, Homma M.

J Mol Biol 414 巻 ( 1 ) 頁： 62-74 2011年11月

Mutations Targeting the C-Terminal Domain of FliG Can Disrupt Motor Assembly in the Na+-Driven Flagella of Vibrio alginolyticus 査読有り

Kojima, S; Nonoyama, N; Takekawa, N; Fukuoka, H; Homma, M

JOURNAL OF MOLECULAR BIOLOGY 414 巻 ( 1 ) 頁： 62 - 74 2011年11月

Single cell adhesion force measurement for cell viability identification using an AFM cantilever-based micro putter 査読有り

Shen, YJ; Nakajima, M; Kojima, S; Homma, M; Kojima, M; Fukuda, T

MEASUREMENT SCIENCE AND TECHNOLOGY 22 巻 ( 11 ) 2011年11月

Sodium-driven motor of the polar flagellum in marine bacteria Vibrio 査読有り

Li, N; Kojima, S; Homma, M

GENES TO CELLS 16 巻 ( 10 ) 頁： 985 - 999 2011年10月

細菌べん毛モーターエネルギー変換タンパク質の構造と機能招待有り査読有り

寺内尭史、小嶋誠司、本間道夫

生化学 83 巻 ( 9 ) 頁： 822-833 2011年9月

A conserved residue, PomB-F22, in the transmembrane segment of the flagellar stator complex, has a critical role in conducting ions and generating torque 査読有り

Terauchi, T; Terashima, H; Kojima, S; Homma, M

MICROBIOLOGY-SGM 157 巻 ( Pt 8 ) 頁： 2422 - 2432 2011年8月

Characterization of the Periplasmic Region of PomB, a Na+-Driven Flagellar Stator Protein in Vibrio alginolyticus 査読有り

Li, N; Kojima, S; Homma, M

JOURNAL OF BACTERIOLOGY 193 巻 ( 15 ) 頁： 3773 - 3784 2011年8月

Design and characterization of nanoknife with buffering beam for in situ single-cell cutting 査読有り国際誌

Shen, YJ; Nakajima, M; Yang, Z; Kojima, S; Homma, M; Fukuda, T

NANOTECHNOLOGY 22 巻 ( 30 ) 頁： 305701 - 305701 2011年7月

Effect of ambient humidity on the strength of the adhesion force of single yeast cell inside environmental-SEM 査読有り

Shen, YJ; Nakajima, M; Ahmad, MR; Kojima, S; Homma, M; Fukuda, T

ULTRAMICROSCOPY 111 巻 ( 8 ) 頁： 1176 - 1183 2011年7月

Study of the time effect on the strength of cell-cell adhesion force by a novel nano-picker 査読有り

Shen, YJ; Nakajima, M; Kojima, S; Homma, M; Fukuda, T

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 409 巻 ( 2 ) 頁： 160 - 165 2011年6月

M153R Mutation in a pH-Sensitive Green Fluorescent Protein Stabilizes Its Fusion Proteins 査読有り

Morimoto, YV; Kojima, S; Namba, K; Minamino, T

PLOS ONE 6 巻 ( 5 ) 頁： e19598 2011年5月

Buckling Nanoneedle for Characterizing Single Cells Mechanics Inside Environmental SEM 査読有り

Ahmad, MR; Nakajima, M; Kojima, S; Homma, M; Fukuda, T

IEEE TRANSACTIONS ON NANOTECHNOLOGY 10 巻 ( 2 ) 頁： 226 - 236 2011年3月

Sodium-driven motor of the polar flagellum in marine bacteria Vibrio. 査読有り

Li N, Kojima S, Homma M.

Genes to Cells 16 巻 ( 10 ) 頁： 985-999 2011年

Effect of ambient humidity on the strength of the adhesion force of single yeast cell inside environmental-SEM. 査読有り

Shen Y, Nakajima M, Ahmad MR, Kojima S, Homma M, Fukuda T.

Ultramicroscopy 111 巻 ( 8 ) 頁： 1176-1183 2011年

Design and characterization of nanoknife with buffering beam for in situ single-cell cutting. 査読有り

Shen Y, Nakajima M, Yang Z, Kojima S, Homma M, Fukuda T.

Nanotechnology 22 巻 ( 30 ) 頁： 305701 2011年

A conserved residue, PomB-F22, in the transmembrane segment of the flagellar stator complex, has a critical role in conducting ions and generating torque. 査読有り

Terauchi T, Terashima H, Kojima S, Homma M.

Microbiology 157 巻頁： 2422-2432 2011年

Characterization of the periplasmic region of PomB, a Na+-driven flagellar stator protein in Vibrio alginolyticus. 査読有り

Li N, Kojima S, Homma M.

J Bacteriol 193 巻 ( 15 ) 頁： 3773-3784 2011年

Study of the time effect on the strength of cell-cell adhesion force by a novel nano-picker. 査読有り

Shen Y, Nakajima M, Kojima S, Homma M, Fukuda T.

Biochem Biophys Res Commun. 409 巻 ( 2 ) 頁： 160-165 2011年

M153R Mutation in a pH-Sensitive Green Fluorescent Protein Stabilizes Its Fusion Proteins 査読有り

Morimoto YV, Kojima S, Namba K, Minamino T

PLoS One 6 巻 ( 5 ) 頁： e19598 2011年

Characterization of the flagellar motor composed of functional GFP-fusion derivatives of FliG in the Na(+)-driven polar flagellum of Vibrio alginolyticus. 査読有り

Koike M, Nishioka N, Kojima S, Homma M

Biophysics (Nagoya-shi, Japan) 7 巻頁： 59 - 67 2011年

Fabrication and Application of Nanofork for Measuring Single Cells Adhesion Force inside ESEM 査読有り

Ahmad, MR; Nakajima, M; Kojima, M; Kojima, S; Homma, M; Fukuda, T

ENABLING SCIENCE AND NANOTECHNOLOGY 1341 巻頁： 304 - + 2011年

[Structure and function of energy transduction protein complex of bacterial flagellar motor]. 査読有り

Terauchi T, Kojima S, Homma M

Seikagaku. The Journal of Japanese Biochemical Society 83 巻 ( 9 ) 頁： 822 - 833 2011年

The Flagellar Basal Body-Associated Protein FlgT Is Essential for a Novel Ring Structure in the Sodium-Driven Vibrio Motor 査読有り

Terashima, H; Koike, M; Kojima, S; Homma, M

JOURNAL OF BACTERIOLOGY 192 巻 ( 21 ) 頁： 5609 - 5615 2010年11月

Disulphide cross-linking between the stator and the bearing components in the bacterial flagellar motor 査読有り

Hizukuri, Y; Kojima, S; Homma, M

JOURNAL OF BIOCHEMISTRY 148 巻 ( 3 ) 頁： 309 - 318 2010年9月

Functional Transfer of an Essential Aspartate for the Ion-binding Site in the Stator Proteins of the Bacterial Flagellar Motor 査読有り

Terashima, H; Kojima, S; Homma, M

JOURNAL OF MOLECULAR BIOLOGY 397 巻 ( 3 ) 頁： 689 - 696 2010年4月

Nanoindentation Methods to Measure Viscoelastic Properties of Single Cells Using Sharp, Flat, and Buckling Tips Inside ESEM 査読有り

Ahmad, MR; Nakajima, M; Kojima, S; Homma, M; Fukuda, T

IEEE TRANSACTIONS ON NANOBIOSCIENCE 9 巻 ( 1 ) 頁： 12 - 23 2010年3月

Isolation of Basal Bodies with C-Ring Components from the Na+-Driven Flagellar Motor of Vibrio alginolyticus 査読有り

Koike, M; Terashima, H; Kojima, S; Homma, M

JOURNAL OF BACTERIOLOGY 192 巻 ( 1 ) 頁： 375 - 378 2010年1月

Isolation of basal bodies with C-ring components from the Na+-driven flagellar motor of Vibrio alginolyticus. 査読有り

Koike M., Terashima H., Kojima S. & Homma M.

Journal of Bacteriology 192 巻頁： 375-378 2010年

Functional transfer of an essential aspartate for the ion-binding site in the stator proteins of the bacterial flagellar motor. 査読有り

Terashima, H., Kojima, S & Homma M.

Journal of Molecular Biology 397 巻頁： 689-696 2010年

Disulphide cross-linking between the stator and the bearing components in the bacterial flagellar motor. 査読有り

Hizukuri Y., Kojima, S. & Homma, M.

Journal of Biochemistry (Tokyo) 148 巻頁： 309-318 2010年

The flagellar basal body-associated protein FlgT is essential for a novel ring structure in the sodium-driven Vibrio motor. 査読有り

Terashima H., Koike M., Kojima S. & Homma M.

Journal of Bacteriology 192 巻頁： 5609-5615 2010年

Interaction between Na+ Ion and Carboxylates of the PomA-PomB Stator Unit Studied by ATR-FTIR Spectroscopy 査読有り

Sudo, Y; Kitade, Y; Furutani, Y; Kojima, M; Kojima, S; Homma, M; Kandori, H

BIOCHEMISTRY 48 巻 ( 49 ) 頁： 11699 - 11705 2009年12月

Rotational Speed Control of Na+-Driven Flagellar Motor by Dual Pipettes 査読有り

Nogawa, K; Kojima, M; Nakajima, M; Kojima, S; Homma, M; Fukuda, T

IEEE TRANSACTIONS ON NANOBIOSCIENCE 8 巻 ( 4 ) 頁： 341 - 348 2009年12月

Mutational Analysis of the GTP-binding Motif of FlhF which Regulates the Number and Placement of the Polar Flagellum in Vibrio alginolyticus 査読有り

Kusumoto, A; Nishioka, N; Kojima, S; Homma, M

JOURNAL OF BIOCHEMISTRY 146 巻 ( 5 ) 頁： 643 - 650 2009年11月

Stator assembly and activation mechanism of the flagellar motor by the periplasmic region of MotB 査読有り

Kojima, S; Imada, K; Sakuma, M; Sudo, Y; Kojima, C; Minamino, T; Homma, M; Namba, K

MOLECULAR MICROBIOLOGY 73 巻 ( 4 ) 頁： 710 - 718 2009年8月

The peptidoglycan-binding (PGB) Domain of the Escherichia coli Pal Protein can also Function as the PGB Domain in E. coli Flagellar Motor Protein MotB 査読有り

Hizukuri, Y; Morton, JF; Yakushi, T; Kojima, S; Hommay, M

JOURNAL OF BIOCHEMISTRY 146 巻 ( 2 ) 頁： 219 - 229 2009年8月

Sodium-dependent dynamic assembly of membrane complexes in sodium-driven flagellar motors 査読有り

Fukuoka, H; Wada, T; Kojima, S; Ishijima, A; Homma, M

MOLECULAR MICROBIOLOGY 71 巻 ( 4 ) 頁： 825 - 835 2009年2月

Sodium-dependent dynamic assembly of membrane complexes in sodium-driven flagellar motors. 査読有り

Fukuoka, H., Wada, T., Kojima, S., Ishijima, A. & Homma, M.

Molecular Microbiology 71 巻頁： 825-835 2009年

Mutational analysis of the GTP-binding motif of FlhF which regulates the number and placement of the polar flagellum in Vibrio alginolyticus. 査読有り

Kusumoto, A., Nishioka, N., Kojima, S. & Homma, M.

Journal of Biochemistry (Tokyo) 146 巻頁： 643-650 2009年

*Stator assembly and activation mechanism of the flagellar motor by the periplasmic region of MotB. 査読有り

Kojima, S., Imada, K., Sakuma, M., Sudo, Y., Kojima, C., Minamino, T., Homma, M. & Namba, K.

Molecular Microbiology 73 巻頁： 710-718 2009年

Comparative study of the ion flux pathway in stator units of proton- and sodium-driven flagellar motors. 査読有り

Sudo, Y., Terashima, H., Abe-Yoshizumi, R., Kojima, S. & Homma, M.

Biophysics 5 巻頁： 45-52 2009年

The peptidoglycan-binding (PGB) domain of the Escherichia coli Pal protein can also function as the PGB domain in E. coli flagellar motor protein MotB. 査読有り

Hizukuri, Y., Morton, J.F., Yakushi, T., Kojima, S. & Homma, M.

Journal of Biochemistry (Tokyo) 146 巻頁： 219-229 2009年

Comparative study of the ion flux pathway in stator units of proton- and sodium-driven flagellar motors. 査読有り

Sudo Y, Terashima H, Abe-Yoshizumi R, Kojima S, Homma M

Biophysics (Nagoya-shi, Japan) 5 巻頁： 45 - 52 2009年

Rotational Speed Control of Na+-driven Flagellar Motor by Nano/Micro Dual Pipettes

Nogawa Kousuke, Kojima Masaru, Nakajima Masahiro, Kojima Seiji, Homma Michio, Fukuda Toshio

2009 9TH IEEE CONFERENCE ON NANOTECHNOLOGY (IEEE-NANO) 頁： 522 - 525 2009年

Cell-free Synthesis of the Torque-Generating Membrane Proteins, PomA and PomB, of the Na+-driven Flagellar Motor in Vibrio alginolyticus 査読有り

Terashima, H; Abe-Yoshizumi, R; Kojima, S; Homma, M

JOURNAL OF BIOCHEMISTRY 144 巻 ( 5 ) 頁： 635 - 642 2008年11月

Suppressor analysis of the MotB(D33E) mutation to probe bacterial flagellar motor dynamics coupled with proton translocation 査読有り

Che, YS; Nakamura, S; Kojima, S; Kami-Ike, N; Namba, K; Minamino, T

JOURNAL OF BACTERIOLOGY 190 巻 ( 20 ) 頁： 6660 - 6667 2008年10月

In Situ Single Cell Mechanics Characterization of Yeast Cells Using Nanoneedles Inside Environmental SEM

Ahmad, MR; Nakajima, M; Kojima, S; Homma, M; Fukuda, T

IEEE TRANSACTIONS ON NANOTECHNOLOGY 7 巻 ( 5 ) 頁： 607 - 616 2008年9月

The effects of cell sizes, environmental conditions, and growth phases on the strength of individual W303 yeast cells inside ESEM 査読有り

Ahmad, MR; Nakajima, M; Kojima, S; Homma, M; Fukuda, T

IEEE TRANSACTIONS ON NANOBIOSCIENCE 7 巻 ( 3 ) 頁： 185 - 193 2008年9月

Insights into the stator assembly of the Vibrio flagellar motor from the crystal structure of MotY 査読有り

Kojima, S; Shinohara, A; Terashima, H; Yakushi, T; Sakuma, M; Homma, M; Namba, K; Imada, K

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 105 巻 ( 22 ) 頁： 7696 - 7701 2008年6月

Characterization of the periplasmic domain of MotB and implications for its role in the stator assembly of the bacterial flagellar motor 査読有り

Kojima, S; Furukawa, Y; Matsunarni, H; Minamino, T; Narnba, K

JOURNAL OF BACTERIOLOGY 190 巻 ( 9 ) 頁： 3314 - 3322 2008年5月

Roles of charged residues in the C-terminal region of PomA, a stator component of the Na+-driven flagellar motor 査読有り

Obara, M; Yakushi, T; Kojima, S; Homma, M

JOURNAL OF BACTERIOLOGY 190 巻 ( 10 ) 頁： 3565 - 3571 2008年5月

Collaboration of FlhF and FlhG to regulate polar-flagella number and localization in Vibrio alginolyticus 査読有り

Kusumoto, A; Shinohara, A; Terashima, H; Kojima, S; Yakushi, T; Homma, M

MICROBIOLOGY-SGM 154 巻 ( Pt 5 ) 頁： 1390 - 1399 2008年5月

Systematic Cys mutagenesis of FIgI, the flagellar P-ring component of Escherichia coli 査読有り

Hizukuri, Y; Kojima, S; Yakushi, T; Kawagishi, I; Homma, M

MICROBIOLOGY-SGM 154 巻 ( Pt 3 ) 頁： 810 - 817 2008年3月

極毛単べん毛のビブリオ菌から周毛多べん毛の変異体の単離とその解析

西岡典子, 楠本晃子, 小嶋誠司, 本間道夫, 小嶋勝

日本細菌学雑誌 63 巻 ( 1 ) 頁： 100 - 100 2008年2月

Flagellar motility in bacteria: Structure and function of flagellar motor 招待有り査読有り

Terashima H, Kojima S, Homma M

International review of cell and molecular biology 270 巻頁： 39-85 2008年

Cell-free synthesis of the torque-generating membrane proteins, PomA and PomB, of the Na+-driven flagellar motor in Vibrio alginolyticus. 査読有り

Terashima H, Yoshizumi R, Kojima S, Homma M.

Journal of Biochemistry (Tokyo) 144 巻頁： 635-642 2008年

Flagellar motility in bacteria structure and function of flagellar motor. 招待有り査読有り

Terashima, H., Kojima, S. & Homma, M.

International Review of Cell and Molecular Biology 270 巻頁： 39-85 2008年

Suppressor analysis of the MotB(D33E) mutation to probe bacterial flagellar motor dynamics coupled with proton translocation. 査読有り

Che YS, Nakamura S, Kojima S, Kami-ike N, Namba K, Minamino T.

J. Bacteriol. 190 巻頁： 6660-6667 2008年

The effects of cell sizes, environmental conditions, and growth phases on the strength of individual W303 yeast cells inside ESEM. 査読有り

Ahmad MR, Nakajima M, Kojima S, Homma M, Fukuda T.

IEEE Trans Nanobioscience 7 巻頁： 185-193 2008年

*Insights into the stator assembly of the Vibrio flagellar motor from the crystal structure of MotY. 査読有り

Kojima S, Shinohara A, Terashima H, Yakushi T, Sakuma M, Homma M, Namba K, Imada K.

Proc. Natl. Acad. Sci. USA 105 巻頁： 7696-7701 2008年

*Characterization of the periplasmic domain of MotB and implications for its role in the stator assembly of the bacterial flagellar motor. 査読有り

Kojima S, Furukawa Y, Matsunami H, Minamino T, Namba K.

Journal of Bacteriology 190 巻頁： 3314-3322 2008年

Collaboration of FlhF and FlhG to regulate polar-flagella number and localization in Vibrio alginolyticus. 査読有り

Kusumoto A, Shinohara A, Terashima H, Kojima S, Yakushi T, Homma M.

Microbiology 154 巻頁： 1390-1399 2008年

Roles of charged residues in the C-terminal region of PomA, a stator component of the Na+-driven flagellar motor. 査読有り

Obara M, Yakushi T, Kojima S, Homma M.

Journal of Bacteriology 190 巻頁： 3565-3571 2008年

Systematic Cys mutagenesis of FlgI, the flagellar P-ring component of Escherichia coli. 査読有り

Hizukuri Y, Kojima S, Yakushi T, Kawagishi I, Homma M.

Microbiology 154 巻頁： 810-817 2008年

FLAGELLAR MOTILITY IN BACTERIA: STRUCTURE AND FUNCTION OF FLAGELLAR MOTOR 査読有り

Terashima, H; Kojima, S; Homma, M

INTERNATIONAL REVIEW OF CELL AND MOLECULAR BIOLOGY, VOL 270 270 巻頁： 39 - 85 2008年

Single evaluation of C. Elegans inside environmental scanning electron microscope 査読有り

Masahiro Nakajima, Mohd Ridzuan Ahmad, Seiji Kojima, Michio Homma, Toshio Fukuda

2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008 頁： 83 - 88 2008年

記述言語：英語掲載種別：研究論文（国際会議プロシーディングス）

This paper presents the single evaluation of Caenorhabditìs Elegans (C. Elegans) by the nanorobotic manipulation system inside an Environmental-Scanning Electron Microscope (E-SEM). C. Elegans has complex outer and inner structures constructed by approximately one thousand cells. Their fine structures were observed by E-SEM directly
without any drying or dyeing processes. For example, the lateral alae are the surface mark of seam cell body. The observation environments are controlled under different E-SEM chamber pressures for clear observation of C. Elegans. The local stiffness evaluation method of C. Elegans was proposed by buckling nanoprobe. The Silicon nanoprobe was fabricated by Focus Ion Beam (FIB) etching at the tip of Atomic Force Microscope (AFM) cantilever. The measurement position can arbitrarily be controlled by the nanorobotic manipulator inside the E-SEM. The local stiffness on or around the lateral alae evaluated from the experimental results. This local stiffness measurement technique can readily be applied to reveal unknown biological local stiffness, cell health conditions and novel cell diagnosis. © 2008 IEEE.

DOI： 10.1109/MHS.2008.4752427

Scopus

Na+駆動型べん毛モーターにおける遊泳速度の圧力応答(Pressure-velocity relationship of sodium-driven flagellar motor of Vibrio alginolyticus)

下田義樹, 西山雅祥, 小嶋誠司, 本間道夫, 木村佳文, 寺嶋正秀

生物物理 47 巻 ( Suppl.1 ) 頁： S247 - S247 2007年11月

Visualization of functional rotor proteins of the bacterial flagellar motor in the cell membrane 査読有り

Fukuoka, H; Sowa, Y; Kojima, S; Ishijima, A; Homma, M

JOURNAL OF MOLECULAR BIOLOGY 367 巻 ( 3 ) 頁： 692 - 701 2007年3月

Visualization of functional rotor proteins of the bacterial flagellar motor in the cell membrane 査読有り

Fukuoka H, Sowa Y, Kojima S, Ishijima A and Homma M

Journal of Molecular Biology 367 巻 ( 3 ) 頁： 692-701 2007年3月

Crystallization and preliminary X-ray analysis of MotY, a stator component of the Vibrio alginolyticus polar flagellar motor 査読有り

Shinohara A, Sakuma M, Yakushi T, Kojima S, Namba K Homma M and Imada K

Acta Crystallograph Sect F 63 巻頁： 89-92 2007年2月

Crystallization and preliminary X-ray analysis of MotY, a stator component of the Vibrio alginolyticus polar flagellar motor 査読有り

Shinohara, A; Sakuma, M; Yakushi, T; Kojima, S; Namba, K; Homma, M; Imada, K

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 63 巻 ( Pt 2 ) 頁： 89 - 92 2007年2月

The Vibrio motor proteins, MotX and MotY, are associated with the basal body of Na-driven flagella and required for stator formation 査読有り

Terashima H, Fukuoka H, Yakushi T, Kojima S and Homma M

Molecular Microbiology 62 巻 ( 4 ) 頁： 1170-1180 2006年11月

The Vibrio motor proteins, MotX and MotY, are associated with the basal body of Na+-driven flagella and required for stator formation 査読有り

Terashima, H; Fukuoka, H; Yakushi, T; Kojima, S; Homma, M

MOLECULAR MICROBIOLOGY 62 巻 ( 4 ) 頁： 1170 - 1180 2006年11月

[Topology of bacterial cells focused on polar-localized proteins]. 査読有り

Kusumoto A, Kojima S, Homma M

Nihon saikingaku zasshi. Japanese journal of bacteriology 61 巻 ( 3 ) 頁： 325 - 337 2006年8月

Arrangement of core membrane segments in the MotA/MotB proton-channel complex of Escherichia coli 査読有り

TF Braun, LQ Al-Mawsawi, S Kojima, DF Blair

BIOCHEMISTRY 43 巻 ( 1 ) 頁： 35 - 45 2004年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：AMER CHEMICAL SOC

The stator of the bacterial flagellar motor is formed from the membrane proteins MotA and MotB, which associate in complexes with stoichiometry MotA(4)MotB(2) (Kojima, S., and Blair, D. F., preceding paper in this issue). The MotA/MotB complexes conduct ions across the membrane, and couple ion flow to flagellar rotation by a mechanism that appears to involve conformational changes within the complex. MotA has four membrane-crossing segments, termed A1-A4, and MotB has one, termed B. We are studying the organization of the 18 membrane segments in the MotA(4)MotB(2) complex by using targeted disulfide cross-linking. A previous cross-linking study showed that the two B segments in the complex (one from each MotB subunit) are arranged as a symmetrical dimer of alpha-helices. Here, we extend the cross-linking study to segments A3 and A4. Single Cys residues were introduced by mutation in several consecutive positions in segments A3 and A4, and double mutants were made by pairwise combination of subsets of the Cys replacements in segments A3, A4, and B. Disulfide cross-linking of the single- and double-Cys proteins was studied in whole cells, in membranes, and in detergent solution. Several combinations of Cys residues in segments A3 and B gave a high yield of disulfide-linked MotA/MotB heterodimer upon oxidation with iodine. Positions of efficient cross-linking identify a helix face on segment A3 that is in proximity to segment(s) B. Some combinations of Cys residues in segments A4 and B also gave a significant yield of disulfide-linked heterodimer, indicating that segment A4 is also near segment(s) B. Certain combinations of Cys residues in segments A3 and A4 cross-linked to form MotA tetramers in high yield upon oxidation. The high-yield positions identify faces on A3 and A4 that are at an interface between MotA subunits. Taken together with mutational studies and patterns of amino acid conservation, the cross-linking results delineate the overall arrangement of 10 membrane segments in the MotA/MotB complex, and identify helix faces likely to line the proton channels.

DOI： 10.1021/bi035406d

Web of Science

PubMed

Solubilization and purification of the MotA/MotB complex of Escherichia coli 査読有り

S Kojima, DF Blair

BIOCHEMISTRY 43 巻 ( 1 ) 頁： 26 - 34 2004年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：AMER CHEMICAL SOC

Bacterial flagella are driven at their base by a rotary motor fueled by the membrane gradient of protons or sodium ions. The stator of the flagellar motor is formed from the membrane proteins MotA and MotB, which function together to conduct ions across the membrane and couple ion flow to rotation. An invariant aspartate residue in MotB (Asp32 in the protein of E. coli) is essential for rotation and appears to have a direct role in proton conduction. A recent study showed that changes at Asp32 in MotB cause a conformational change in the complex, as evidenced by altered patterns of protease susceptibility of MotA [Kojima, S., and Blair, D. F. (2001) Biochemistry 40 (43), 13041-13050]. It was proposed that protonation/deprotonation of Asp32 might regulate a conformational change in the stator that acts as the powerstroke to drive rotation of the rotor. Biochemical studies of the MotA/MotB complex have been hampered by the absence of a suitable assay for its integrity in detergent solution. Here, we have studied the behavior of the MotA/MotB complex in a variety of detergents, making use of the protease-susceptibility assay to monitor its integrity. Among about 25 detergents tested, a few were found to solubilize the proteins effectively while preserving certain conformational properties characteristic of an intact complex. The detergent dodecylphosphocholine, or DPC, proved especially effective. MotA/MotB complexes purified in DPC migrate with an apparent size of similar to300 kDa in gel-filtration columns, and retain the Asp32-modulated conformational differences seen in membranes. S-35-radiolabeling showed that MotA and MotB are present in a 2:1 ratio in the complex. Purified MotA/MotB complexes should enable in vitro study of the proton-induced conformational change and other aspects of stator function.

DOI： 10.1021/bi0354051

Web of Science

PubMed

Solubilization and purification of the MotA/MotB complex of Escherichia coli 査読有り

Kojima, S., and Blair, D.F.

Biochemistry 43 巻頁： 26-35 2004年

The bacterial flagellar motor: Structure and function of a complex molecular machine. 招待有り

Kojima, S., & Blair, D.F.

International Review of Cytology 233 巻頁： 93-134 2004年

Arrangement of core membrane segments in the MotA/MotB proton-channel complex of Escherichia coli 査読有り

Braun, T.F., Al-Mawsawi, L.Q., Kojima, S., & Blair, D.F.

Biochemistry 43 巻頁： 35-45 2004年

The bacterial flagellar motor: Structure and function of a complex molecular machine 査読有り

S Kojima, DF Blair

INTERNATIONAL REVIEW OF CYTOLOGY - A SURVEY OF CELL BIOLOGY, VOL. 233 233 巻頁： 93 - 134 2004年

Rotary motors (bacterial). 招待有り

Kojima, S., & Blair, D.F.

McGraw-Hill Yearbook of Science & Technology 2003 頁： 363-366 2003年

Conformational change in the stator of the bacterial flagellar motor 査読有り

S Kojima, DF Blair

BIOCHEMISTRY 40 巻 ( 43 ) 頁： 13041 - 13050 2001年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：AMER CHEMICAL SOC

MotA and MotB are integral membrane proteins of Escherichia coli that form the stator of the proton-fueled flagellar rotary motor. The motor contains several MotA/MotB complexes, which function independently to conduct protons across the cytoplasmic membrane and couple proton flow to rotation. MotB contains a conserved aspartic acid residue, Asp32, that is critical for rotation. We have proposed that the protons energizing the motor interact with Asp32 of MotB to induce conformational changes in the stator that drive movement of the rotor. To test for conformational changes, we examined the protease susceptibility of MotA in membrane-bound complexes with either wild-type MotB or MotB mutated at residue 32. Small, uncharged replacements of Asp32 in MotB (D32N, D32A, D32G, D32S, or D32C) caused a significant change in the conformation of MotA, as evidenced by a change in the pattern of proteolytic fragments. The conformational change does not require any flagellar proteins besides MotA and MotB, as it was still seen in a strain that expresses no other flagellar genes. It affects a cytoplasmic domain of MotA that contains residues known to interact with the rotor, consistent with a role in the generation of torque. Influences of key residues of MotA on conformation were also examined. Pro173 of MotA, known to be important for rotation, is a significant determinant of conformation: Dominant Pro173 mutations, but not recessive ones, altered the proteolysis pattern of MotA and also prevented the conformational change induced by Asp32 replacements. Arg90 and Glu98, residues of MotA that engage in electrostatic interactions with the rotor, appear not to be strong determinants of conformation of the MotA/MotB complex in membranes. We note sequence similarity between MotA and ExbB, a cytoplasmic-membrane protein that energizes outer-membrane transport in Gram-negative bacteria. ExbB and associated proteins might also employ a mechanism involving proton-driven conformational change.

Web of Science

*Conformational change in the stator of the bacterial flagellar motor. 査読有り

Kojima, S., & Blair, D.F.

Biochemistry 40 巻頁： 13041-13050 2001年

A slow-motility phenotype caused by substitutions at residue Asp31 in the PomA channel component of a sodium-driven flagellar motor 査読有り

S Kojima, T Shoji, Y Asai, Kawagishi, I, M Homma

JOURNAL OF BACTERIOLOGY 182 巻 ( 11 ) 頁： 3314 - 3318 2000年6月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：AMER SOC MICROBIOLOGY

PomA is thought to be a component of the ion channel in the sodium-driven polar-flagellar motor of Vibrio alginolyticus. We have found that some cysteine substitutions in the periplasmic region of PomA result in a slow-motility phenotype, in which swarming and swimming speeds are reduced even in the presence of high concentrations of NaCl. Most of the mutants showed a sodium ion dependence similar to that of the wild type but with significantly reduced motility at all sodium ion concentrations. By contrast, motility of the D31C mutant showed a sharp dependence on NaCl concentration, with a threshold at 38 mM. The motor of the D31C mutant rotates stably, as monitored by laser dark-field microscopy, suggesting that the mutant PomA protein is assembled normally into the motor complex. Mutational studies of Asp31 suggest that, although this residue is not essential for motor rotation, a negative charge at this position contributes to optimal speed and/or efficiency of the motor.

Web of Science

A slow-motility phenotype caused by substitutions at residue Asp31 in the PomA channel component of a sodium-driven flagellar motor

Kojima S, Shoji T, Asai Y, Kawagishi I, Homma M

JOURNAL OF BACTERIOLOGY 182 巻 ( 11 ) 頁： 3314 - 3318 2000年6月

A slow-motility phenotype caused by substitutions at residue Asp31 in the PomA channel component of a sodium-driven flagellar motor.

Kojima, S., Shoji, T., Asai, Y., Kawagishi, I & Homma, M.

Journal of Bacteriology 182 巻頁： 3314-3318 2000年

Random mutagenesis of the pomA gene encoding a putative channel component of the Na+-driven polar flagellar motor of Vibrio alginolyticus 国際誌

Kojima, S; Kuroda, M; Kawagishi, I; Homma, M

MICROBIOLOGY-UK 145 巻頁： 1759 - 1767 1999年7月

The polar flagellar motor of Vibrio cholerae is driven by an Na+ motive force 査読有り

S Kojima, K Yamamoto, Kawagishi, I, M Homma

JOURNAL OF BACTERIOLOGY 181 巻 ( 6 ) 頁： 1927 - 1930 1999年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：AMER SOC MICROBIOLOGY

Vibrio cholerae is a highly motile bacterium which possesses a single polar flagellum as a locomotion organelle. Motility is thought to be an important factor for the virulence of V. cholerae. The genome sequencing project of this organism is in progress, and the genes that are highly homologous to the essential genes of the Na+-driven polar flagellar motor of Vibrio alginolyticus were found in the genome database of V. cholerae. The energy source of its flagellar motor was investigated, We examined the Na+ dependence and the sensitivity to the Na+ motor-specific inhibitor of the motility of the V. cholerae strains and present the evidence that the polar flagellar motor of V. cholerae is driven by an Na+ motive force.

Web of Science

Na+-driven flagellar motor resistant to phenamil, an amiloride analog, caused by mutations in putative channel components 査読有り

Kojima, S; Asai, Y; Atsumi, T; Kawagishi, I; Homma, M

JOURNAL OF MOLECULAR BIOLOGY 285 巻 ( 4 ) 頁： 1537 - 1547 1999年1月

*Na+-driven flagellar motor resistant to phenamil, an amiloride analog, caused by mutations of putative channel components. 査読有り

Kojima, S., Asai, Y., Atsumi, T., Kawagishi, I. & Homma, M.

Journal of Molecular Biology 285 巻頁： 1537-1547 1999年

The polar flagellar motor of Vibrio cholerae is driven by an Na+ motive force. 査読有り

Kojima, S., Yamamoto, K., Kawagishi, I. & Homma, M.

Journal of Bacteriology 181 巻頁： 1927-1930 1999年

Random mutagenesis of the pomA gene encoding a putative channel component of the Na+-driven polar flagellar motor of Vibrio alginolyticus. 査読有り

Kojima, S., Kuroda, M., Kawagishi, I. & Homma, M.

Microbiology 145 巻頁： 1759-1767 1999年

Putative channel components for the fast-rotating sodium-driven flagellar motor of a marine bacterium 査読有り

Y Asai, S Kojima, H Kato, N Nishioka, Kawagishi, I, M Homma

JOURNAL OF BACTERIOLOGY 179 巻 ( 16 ) 頁： 5104 - 5110 1997年8月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：AMER SOC MICROBIOLOGY

The polar flagellum of Vibrio alginolyticus rotates remarkably fast (up to 1,700 revolutions per second) by using a motor driven by sodium ions, Two genes, motX and motY, for the sodium-driven flagellar motor have been identified in marine bacteria, Vibrio parahaemolyticus and V. alginolyticus. They have no similarity to the genes for proton-driven motors, motA and motB, whose products constitute a proton channel, MotX Has proposed to be a component of a sodium channel, Were we identified additional sodium motor genes, pomA and pomB, in V. alginolyticus. Unexpectedly, PomA and PomB have similarities to MotA and MotB, respectively, especially in the predicted transmembrane regions, These results suggest that PomA and PomB may be sodium-conducting channel components of the sodium-driven motor and that the motor part consists of the products of at least four genes, pomA, pomB, motX, and motY. Furthermore, swimming speed was controlled by the expression level of the pomA gene. suggesting that newly synthesized PomA proteins, which are components of a force-generating unit, were successively integrated into the defective motor complexes. These findings imply that Na+-driven flagellar motors mag have similar structure and function as proton driven motors, but with some interesting differences as well, and it is possible to compare and study the coupling mechanisms of the sodium and proton ion flux for the force generation.

Web of Science

Putative channel components for the fast-rotating sodium-driven flagellar motor of a marine bacterium

Asai Y, Kojima S, Kato H, Nishioka N, Kawagishi I, Homma M

JOURNAL OF BACTERIOLOGY 179 巻 ( 16 ) 頁： 5104 - 5110 1997年8月

Characterization of polar-flagellar-length mutants in Vibrio alginolyticus 査読有り

Furuno, M; Atsumi, T; Yamada, T; Kojima, S; Nishioka, N; Kawagishi, I; Homma, M

MICROBIOLOGY-SGM 143 巻 ( 5 ) 頁： 1615 - 1621 1997年5月

Vibrio alginolyticus mutants resistant to phenamil, a specific inhibitor of the sodium-driven flagellar motor

Kojima, S; Atsumi, T; Muramoto, K; Kudo, S; Kawagishi, I; Homma, M

JOURNAL OF MOLECULAR BIOLOGY 265 巻 ( 3 ) 頁： 310 - 318 1997年1月

Characterization of the polar-flagellar length mutants in Vibrio alginolitycus. 査読有り

Furuno, M., Atsumi, T., Kojima, S., Nishioka, N., Kawagishi, I. & Homma, M.

Microbiology 143 巻頁： 1615-1621 1997年

Vibrio alginolyticus mutants resistant to phenamil, a specific inhibitor of the sodium-driven flagellar motor. 査読有り

Kojima, S., Atsumi, T., Muramoto, K., Kudo, S., Kawagishi, I. & Homma, M.

Journal of Molecular Biology 265 巻頁： 310-318 1997年

Putative channel components for the fast-rotating sodium-driven flagellar motor of a marine bacterium. 査読有り

Asai, Y., Kojima, S., Kato, H., Nishioka, N., Kawagishi, I. & Homma, M.

Journal of Bacteriology 179 巻頁： 5104-5110 1997年

バクテリアのNa^+駆動型モーターとべん毛運動

小嶋誠司, 川岸郁朗, 本間道夫

化学と生物 34 巻 ( 11 ) 頁： 730 - 737 1996年11月

Chemotactic responses to an attractant and a repellent by the polar and lateral flagellar systems of Vibrio alginolyticus 査読有り

M Homma, H Oota, S Kojima, Kawagishi, I, Y Imae

MICROBIOLOGY-UK 142 巻頁： 2777 - 2783 1996年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：SOC GENERAL MICROBIOLOGY

Chemotactic responses in Vibrio alginolyticus, which has lateral and polar flagellar systems in one cell, were investigated. A lateral-flagella-defective (Pof(+) Laf(-)) mutant, which has only a polar flagellum, usually swam forward by the pushing action of its flagellum and occasionally changed direction by backward swimming. When the repellent phenol was added, Pof(+) Laf(-) cells moved frequently forward and backward (tumbling state). The tumbling was derived from the frequent changing between counter-clockwise and clockwise (CW) rotation of the flagellar motor, as was confirmed by the tethered-cell method. Furthermore, we found that the tumbling cells did not adapt to the phenol stimulus. When the attractant serine was added, the phenol-treated cells ceased tumbling and swam smoothly, adapting to the attractant stimulus after several minutes. We isolated chemotaxis-defective (Che(-)) mutants from the Pof(+) Laf(-) mutant; the tumbling mutants were not isolated. One interesting mutant swam backwards continuously, with its flagellum leading the cell and its flagellar motor rotating CW continuously. A polar-flagella-defective mutant (Pof(-) Laf(+)) stopped swimming after phenol addition and then recovered swimming ability within 10 min, indicating that lateral flagella can adapt to the repellent stimulus. This may represent a functional difference between the two flagellar systems in Vibrio cells, and between the chemotaxis systems affecting the two types of flagella.

Web of Science

Chemotactic responses to an attractant and a repellent by the polar and lateral flagellar systems of Vibrio alginolyticus

Homma M, Oota H, Kojima S, Kawagishi I, Imae Y

MICROBIOLOGY-UK 142 巻頁： 2777 - 2783 1996年10月

Chemotactic responses to an attractant and a repellent by the polar and lateral flagellar systems of Vibrio alginolyticus.

Homma, M., Oota, H., Kojima, S., Kawagishi, I. & Imae, Y.

Microbiology 142 巻頁： 2777-2783 1996年

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