研究者詳細 - 浅沼　浩之

論文 - 浅沼　浩之

分割表示 260 件中 121 - 140 件目／全件表示 >>

Signal Amplification Circuit Composed of Serinol Nucleic Acid for RNA Detection 査読有り

Chen, Y.; Murayama, K.; Asanuma, H.

Chem. Lett. 51 巻頁： 330 - 333 2022年1月

Renewable DNA proportional-integral controller with photoresponsive molecules 招待有り査読有り

Tamba, M.; Murayama, K.; Asanuma, H.; Nakakuki, T.

Micromachines 13 巻頁： 193 2022年1月

Unexpected Dissociation of Photoresponsive UV-ON DNA Carrying p-tert-Butyl Azobenzene under UV Light Irradiation 査読有り

Ishii, S.; Murayama, K.; Sada, K.; Asanuma, H.; Kakugo, A.

Chemistry Letters 51 巻頁： 292 - 295 2022年1月

Pseudo base pairs that exhibit high duplex stability and orthogonality through covalent and non─covalent interactions 招待有り

樫田啓, 浅沼浩之

有機合成化学協会誌 79 巻 ( 11 ) 頁： 1013 - 1019 2021年11月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：公益社団法人有機合成化学協会

Natural base pairs stabilize nucleic acid duplexes via hydrogen bonding and stacking interactions, and efforts have been made to identify artificial base pairs that stabilize duplexes through different interactions. Herein, we describe our attempts to prepare pseudo base pairs that exhibit high stability and orthogonality. Pairs of azobenzene derivatives showed unexpectedly high stability and orthogonality due to intermolecular stacking interactions. Pairs of cationic molecules showed even higher stability than natural G–C pairs through both electrostatic and stacking interactions. Duplexes can be crosslinked via [2+2] photocycloaddition between stilbene derivatives, and pairs of non–planar cyclohexane derivatives can stabilize duplexes by hydrophobic interactions rather than stacking interactions. Hetero–selective pairing can be achieved using electron donor–acceptor interactions. These pseudo base pairs would work well as building blocks in nanotechnology and biotechnology due to their unique functionalities.

DOI： 10.5059/yukigoseikyokaishi.79.1013

Scopus

CiNii Research

光駆動型DNAナノマシン招待有り査読有り

浅沼浩之, 村山恵司, 神谷由紀子, 樫田　啓

高分子 70 巻頁： 550 - 552 2021年10月

Preparation of Artificial Hexaplex Composed of Non-Natural Nucleic Acid 招待有り査読有り

Makino K., Hattori Y., Kashida H., Asanuma H.

Current Protocols 1 巻 ( 4 ) 頁： e106 2021年4月

記述言語：日本語出版者・発行元：Current Protocols

This article describes synthetic procedures for acyclic D-threoninol nucleic acid tethering of bifacial nucleobases. Because these nucleobases have complementary hydrogen bonding sites on both sides, their oligomers can form a hexaplex. These hexaplexes are suitable for use as metal or pH sensors and as supramolecular motifs. © 2021 Wiley Periodicals LLC. Basic Protocol 1: Synthesis of D-threoninol backbone. Basic Protocol 2: Synthesis of D-aTNA tethering cyanuric acid. Basic Protocol 3: Synthesis of D-aTNA tethering a 2-aminopyrimidine moiety. Basic Protocol 4: Synthesis of D-aTNA tethering a 2,4,6-triaminopyrimidine moiety. Basic Protocol 5: Synthesis of D-aTNA oligomer tethering cyanuric acid, 2-aminopyrimidine, or 2,4,6-triaminopyrimidine.

DOI： 10.1002/cpz1.106

Scopus

PubMed

Microheater-integrated zinc oxide nanowire microfluidic device for hybridization-based detection of target single-stranded DNA 査読有り

Nanotechnology 2021年3月

Development of pre-miRNAs modification with FRET dye pair for intracellular visualization of processing intermediates generated in cells 査読有り

Kamiya, Y.; Kamimoto, H.; Zhu, H. ; Asanuma, H.

Sensors 21 巻頁： 1785 2021年3月

Development and modification of pre-mirnas with a fret dye pair for the intracellular visualization of processing intermediates that are generated in cells 招待有り査読有り

Kamiya Y., Kamimoto H., Zhu H., Asanuma H.

Sensors 21 巻 ( 5 ) 頁： 1 - 14 2021年3月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Sensors

microRNAs (miRNAs) are small non-coding ribonucleic acids (RNAs), which regulate gene expression via the RNA interference (RNAi) system. miRNAs have attracted enormous interest because of their biological significance and disease relationship. In cell systems, the generation of miRNA is regulated by multiple steps: the transfer of primary miRNA from the nucleus to the cy-tosol, the generation of the precursor-miRNA (pre-miRNA), the production of double-stranded RNA from pre-miRNA by the Dicer, the interaction with protein argonaute-2 (AGO2), and the sub-sequent release of one strand to form miRISC with AGO2. In this study, we attempt to visualize the intermediates that were generated in the miRNA-maturation step in the cells to acquire a detailed understanding of the maturation process of miRNA. To achieve this, we developed pre-miRNAs labeling with a Dicer-or AGO2-responsible fluorescence resonance energy transfer (FRET) dye pair. We observed that modifications with the dye at suitable positions did not interfere with the biological activities of pre-miRNAs. Further, imaging analyses employing these pre-miRNAs demon-strated that the processing of pre-miRNA promoted the accumulation of miRNA at the specific foci in the cytosol. The FRET-labeled pre-miRNA would further elucidate the mechanisms of the RNAi process and provide the basis for development of nucleic acid drugs working in the RNAi system.

DOI： 10.3390/s21051785

Scopus

PubMed

Pyrene modified serinol nucleic acid nanostructure converts chirality of threoninol nucleic acids into circularly polarized luminescence signals 招待有り査読有り

Kashida, H.; Nishikawa, K.; Ito Y.; Murayama, K.; Hayashi, I.; Kakuta, T.; Ogoshi, T.; Asanuma, H.

2021年1月

Microheater-integrated zinc oxide nanowire microfluidic device for hybridization-based detection of target single-stranded DNA 査読有り

Takahashi, H.; Yasui, T.; Kashida, H.; Makino, K.; Shinjo, K.; Liu, Q.; Shimada, T.; Rahong, S.; Kaji, N.; Asanuma, H.; Baba, Y.

Nanotechnology 32 巻頁： 255301 2021年

核酸医薬の創製に向けた人工核酸の開発招待有り査読有り

樫田啓、村山恵司、浅沼浩之

バイオマテリアル 38 巻 ( 2 ) 頁： 124 - 129 2020年4月

The Use of Serinol Nucleic Acids as Ultrasensitive Molecular Beacons 招待有り査読有り

Murayama Keiji, Kashida Hiromu, Asanuma Hiroyuki

NON-NATURAL NUCLEIC ACIDS: METHODS AND PROTOCOLS 1973 巻頁： 261-279 2019年

Development of Visible-Light-Responsive RNA Scissors Based on a 10-23 DNAzyme 査読有り

Kamiya Yukiko, Arimura Yu, Ooi Hideaki, Kato Kenjiro, Liang Xing-Guo, Asanuma Hiroyuki

CHEMBIOCHEM 19 巻 ( 12 ) 頁： 1305 - 1311 2018年6月

DNA Microcapsule for Photo-Triggered Drug Release Systems

Kamiya Yukiko, Yamada Yoshinobu, Muro Takahiro, Matsuura Kazunori, Asanuma Hiroyuki

CHEMMEDCHEM 12 巻 ( 24 ) 頁： 2016-2021 2017年12月

Introduction of 2,6-Diaminopurines into Serinol Nucleic Acid Improves Anti-miRNA Performance 査読有り

Kamiya Yukiko, Donoshita Yuka, Kamimoto Hiroshi, Murayama Keiji, Ariyoshi Jumpei, Asanuma Hiroyuki

CHEMBIOCHEM 18 巻 ( 19 ) 頁： 1917-1922 2017年10月

Chaperone-Polymer-Assisted, Photodriven DNA Strand Displacement

Cheng Bohao, Kashida Hiromu, Shimada Naohiko, Maruyama Atsushi, Asanuma Hiroyuki

CHEMBIOCHEM 18 巻 ( 16 ) 頁： 1568-1572 2017年8月

D-aTNA Circuit Orthogonal to DNA Can Be Operated by RNA Input via SNA

Murayama Keiji, Nagao Ryuya, Asanuma Hiroyuki

CHEMISTRYSELECT 2 巻 ( 20 ) 頁： 5624-5627 2017年7月

Antisense oligonucleotide modified with serinol nucleic acid (SNA) induces exon skipping in mdx myotubes 査読有り

Le, B. T.; Murayama, K.; Shabanpoor, F.; Asanuma, H.; Veedu, R. N.

RSC Advances 7 巻頁： 34049-34052 2017年7月

光応答性DNAの開発―ナノ環境を汚染しない分子マシンへの応用― 招待有り

浅沼浩之

現代化学 556 巻 ( 7 ) 頁： 50-55 2017年7月

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