研究者詳細 - 浅沼　浩之

論文 - 浅沼　浩之

分割表示 260 件中 1 - 20 件目／全件表示 >>

In Vivo Efficacy and Safety of Systemically Administered Serinol Nucleic Acid-Modified Antisense Oligonucleotides in Mouse Kidney 査読有り

Tsuboi, T.; Hattori, K.; Ishimoto, T.; Imai, K.; Doke, T.; Hagita, J.; Ariyoshi, J.; Furuhashi, K.; Kato, N.; Ito, Y.; Kamiya, Y.; Asanuma, H.; Maruyama, S.

Mol. Ther. Nucleic Acids 36 巻頁： 102387 2025年3月

"Kinetics of Strand Displacement Reaction with Acyclic Artificial Nucleic Acids 査読有り

Makino, K.; Sugiyama, I.; Asanuma, H.; Kashida, H.

Angew. Chem. Int. Ed. Engl. 63 巻頁： e202319864 2024年7月

A Light-powered single-stranded DNA molecular motor with colour-selective single-step control 査読有り国際共著

Anderson, T.; Wu, W.; Sirbu, O.; Tong, K.; Winna, S.; Liu, X. R.; Kou, B.; Murayama, K.; Asanuma, H.; Wang, Z.

Angew. Chem. Int. Ed. Engl. 63 巻頁： e202405250 2024年6月

Site-selective photo-crosslinking of stilbene pairs in a DNA duplex mediated by ruthenium photocatalyst 招待有り査読有り

Kashida, H.; Azuma, H.; Sotome, H.; Miyasaka, H.; Asanuma, H.

Angewandte Chemie International Edition 63 巻頁： e202319516 2024年1月

Unexpectedly stable homopurine parallel triplex of SNA:RNA*SNA and L-aTNA:RNA*L-aTNA 査読有り

Kamiya, Y.; Lao, S.; Ariyoshi, J.; Sato, F.; Asanuma, H.

Chemical Communications 60 巻頁： 1257 - 1260 2023年12月

Rapid Chemical Ligation of DNA and Acyclic Threoninol Nucleic Acid (aTNA) for Effective Nonenzymatic Primer Extension 査読有り

Okita, H.; Kondo, S.; Murayama, K.; Asanuma, H.

Journal of the American Chemical Society 145 巻頁： 17872 - 17880 2023年7月

Syntheses of base-labile pseudo-complementary SNA and L-aTNA phosphoramidite monomers 招待有り査読有り

Sato, F.; Kamiya, Y.; Asanuma, H.

J. Org. Chem. 88 巻頁： 796 - 804 2023年1月

Cooperative cargo transportation by a swarm of molecular machines 査読有り

Akter, M.; Keya, J. J.; Kayano, K.; Kabir, A. M. R.; Inoue, D.; Hess, H.; Sada, K.; Kuzuya, A.; Asanuma, H.; Kakugo, A.

Sci. Robot. 7 巻頁： eabm0677 2022年5月

Xeno nucleic acids (XNAs) having non-ribose scaffolds with unique supramolecular properties 招待有り査読有り

Asanuma, H.; Kamiya, Y.; Kashida, H.; Murayama, K.

28 巻頁： 3933 2022年3月

Color-Changing Fluorescent Barcode Based on Strand Displacement Reaction Enables Simple Multiplexed Labeling 査読有り

Makino, K.; Susaki, E.; Endo, M.; Asanuma, H.; Kashida, H.

Journal of the American Chemical Society 144 巻頁： 1572 - 1579 2022年1月

Orthogonal Amplification Circuits Composed of Acyclic Nucleic Acids Enable RNA Detection 査読有り

Chen Y., Nagao R., Murayama K., Asanuma H.

Journal of the American Chemical Society 144 巻頁： 5887 - 5892 2022年

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Journal of the American Chemical Society

Construction of complex DNA circuits is difficult due to unintended hybridization and degradation by enzymes under biological conditions. We herein report a hybridization chain reaction (HCR) circuit composed of left-handed acyclic d-threoninol nucleic acid (d-aTNA), which is orthogonal to right-handed DNA and RNA. Because of its high thermal stability, use of an aTNA hairpin with a short 7 base-pair stem ensured clear ON-OFF control of the HCR circuit. The aTNA circuit was stable against nucleases. A circuit based on right-handed acyclic l-threoninol nucleic acid (l-aTNA) was also designed, and high orthogonality between d- and l-aTNA HCRs was confirmed by activation of each aTNA HCR via a corresponding input strand. A dual OR logic gate was successfully established using serinol nucleic acid (SNA), which could initiate both d- and l-aTNA circuits. The d-aTNA HCR was used for an RNA-dependent signal amplification system via the SNA interface. The design resulted in 80% yield of the cascade reaction in 3000 s without a significant leak. This work represents the first example of use of heterochiral HCR circuits for detection of RNA molecules. The method has potential for direct visualization of RNA in vivo and the FISH method.

DOI： 10.1021/jacs.1c12659

Scopus

Perylene-Cy3 FRET System to Analyze Photoactive DNA Structures 査読有り

Kawai H., Doi T., Ito Y., Kameyama T., Torimoto T., Kashida H., Asanuma H.

Chemistry - A European Journal 27 巻 ( 50 ) 頁： 12845 - 12850 2021年9月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Chemistry - A European Journal

We report a new Förster resonance energy transfer (FRET) system for structural analyses of DNA duplexes using perylene and Cy3 as donor and acceptor, respectively, linked at the termini of a DNA duplex via D-threoninol. Experimentally obtained FRET efficiencies were in good agreement with theoretical values calculated based on canonical B-form DNA. Due to the relatively long Förster radius, this system can be used to analyze large DNA structures, and duplexes containing photo-reactive molecules can be analyzed since perylene can be excited with visible light. The system was used to analyze a DNA duplex containing stilbene, demonstrating that in the region of the stilbene cluster the duplex adopts a ladder-like structure rather than helical one. Upon photodimerization between stilbene residues, FRET efficiencies indicated the reaction does not disturb DNA duplex. This FRET system will be useful for analysis of photoreactions of nucleobases as well as a wide range of nucleic acid structures.

DOI： 10.1002/chem.202101738

Scopus

PubMed

Design and Hybridization Properties of Acyclic Xeno Nucleic Acid Oligomers 招待有り査読有り

Murayama K., Asanuma H.

ChemBioChem 22 巻 ( 15 ) 頁： 2507 - 2515 2021年8月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ChemBioChem

Xeno nucleic acids (XNAs) are analogues of DNA and RNA that have a non-ribose artificial scaffold. XNAs are possible prebiotic genetic carriers as well as alternative genetic systems in artificial life. In addition, XNA oligomers can be used as biological tools. Acyclic XNAs, which do not have cyclic scaffolds, are attractive due to facile their synthesis and remarkably high nuclease resistance. To maximize the performance of XNAs, a negatively charged backbone is preferable to provide sufficient water solubility; however, acyclic XNAs containing polyanionic backbones suffer from high entropy cost upon duplex formation, because of the high flexibility of the acyclic nature. Herein, we review the relationships between the structure and duplex hybridization properties of various acyclic XNA oligomers with polyanion backbones.

DOI： 10.1002/cbic.202100184

Scopus

PubMed

Dual Crosslinking Photo-Switches for Orthogonal Photo-Control of Hybridization Between Serinol Nucleic Acid and RNA 査読有り

Yamano Y., Murayama K., Asanuma H.

Chemistry - A European Journal 27 巻 ( 14 ) 頁： 4599 - 4604 2021年3月

担当区分：最終著者,　責任著者記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：Chemistry - A European Journal

Wavelength-selective photo-regulation by multiple chromophores responding to different wavelengths can expand the variation of photo-manipulating systems. Herein, we report the orthogonal photo-regulation of duplex formation between serinol nucleic acid (SNA) and RNA using light-induced crosslinking reactions mediated by a new photo-reactive nucleobase 8-naphthylvinyladenine (NVA) and previously described 8-pyrenylvinyladenine (PVA). An intrastrand crosslink was induced in an SNA strand containing two adjacent NVA residues by irradiation with 340–405 nm light; the crosslink was reversed by irradiation with ≤300 nm light. In an SNA strand with adjacent NVA and PVA residues, an intrastrand crosslink resulted from irradiation with 405–465 nm light that was reversed by irradiation with ≤340 nm light. Intrastrand photo-crosslinking caused severe destabilization of an SNA/RNA duplex, resulting in dissociation to single strands. Cycloreversion resulted in duplex formation. With these NVA/NVA and NVA/PVA photo-switches, four hybridization states of two SNA/RNA duplexes could be orthogonally photo-controlled by irradiation with a suitable wavelength of light.

DOI： 10.1002/chem.202003528

Web of Science

Scopus

PubMed

A helical amplification system composed of artificial nucleic acids 査読有り

Kashida H., Nishikawa K., Shi W., Miyagawa T., Yamashita H., Abe M., Asanuma H.

Chemical Science 12 巻 ( 5 ) 頁： 1656 - 1660 2021年2月

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Chemical Science

Herein we report an amplification system of helical excess triggered by nucleic acid hybridization for the first time. It is usually impossible to prepare achiral nanostructures composed of nucleic acids because of their intrinsic chirality. We used serinol nucleic acid (SNA) oligomers for the preparation of achiral nanowires because SNA oligomers with symmetrical sequences are achiral. Nanowire formation was confirmed by atomic force microscopy and size exclusion chromatography. When a chiral nucleic acid with a sequence complementary to SNA was added to the nanostructure, helicity was induced and a strong circular dichroism signal was observed. The SNA nanowire could amplify the helicity of chiral nucleic acids through nucleobase stacks. The SNA nanostructures have potential for use as platforms to detect chiral biomolecules under aqueous conditions because SNA can be readily functionalized and is water-soluble.

DOI： 10.1039/d0sc05245k

Scopus

PubMed

Nonenzymatic polymerase-like template-directed synthesis of acyclic L-threoninol nucleic acid 査読有り

Murayama, K.; Okita, H.; Kuriki, T.; Asanuma, H.

Nature Communications 12 巻頁： 804 2021年2月

Light-Regulated Liquid-Liquid Phase Separation for Spatiotemporal Protein Recruitment and Cell Aggregation 査読有り

Ikeuchi N., Komachi T., Murayama K., Asanuma H., Maruyama A., Shimada N.

ACS Applied Materials and Interfaces 13 巻 ( 4 ) 頁： 5652 - 5659 2021年2月

記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：ACS Applied Materials and Interfaces

We have previously shown that the upper critical solution temperature-type thermoresponsive ureido polymers such as polyallylurea and poly(2-ureidoethylmethacrylate) derivatives show liquid-liquid phase separation (LLPS), also known as simple coacervation, under physiological conditions below their phase-separation temperatures (Tp). The addition of the polymer-rich coacervate droplets that result from LLPS to a monolayer cell culture induced aggregation of cells into multicellular spheroids. In this study, we prepared a ureido copolymer, poly(vinylamine-co-vinylurea), with azobenzene substituents (Azo-PVU) and demonstrated light-guided assembly and disassembly of LLPS coacervates. Azo-PVUs with Tp values ranging from 10 to 52 °C were prepared by changing the azobenzene content. Ultraviolet light caused a decrease in the Tp of Azo-PVU because of trans-to-cis photoisomerization of the azobenzene and irradiation with visible light increased the Tp. Thus, LLPS of Azo-PVU was reversibly controlled. The coacervate droplets deposited on a dish surface were immediately dissolved by targeted UV irradiation (owing to a decrease in the Tp). Spatially controlled recruitment of proteins on the dish surface was achieved when protein solution was added to the light-patterned surface. Furthermore, the light-guided deposition of coacervates resulted in the spatiotemporal transformation of monolayer cells to aggregates. This light-controlled LLPS will allow the preparation of novel liquid-based materials for biomolecular and cellular engineering.

DOI： 10.1021/acsami.0c22314

Scopus

PubMed

Quantitative Analyses of Förster Resonance Energy Transfer between Identical Pyrene Chromophores (Homo-FRET) In DNA Scaffolds. 査読有り

Kashida H., Kawai H., Azuma H., Araki Y., Wada T., Asanuma H.

ChemPhotoChem 5 巻 ( 2 ) 頁： 167 - 172 2021年2月

記述言語：日本語掲載種別：研究論文（学術雑誌）出版者・発行元：ChemPhotoChem

Förster resonance energy transfer between identical chromophores (homo-FRET) has been difficult to analyze since neither emission intensity nor lifetime changes with the occurrence of homo-FRET. Herein we used a DNA scaffold to analyze homo-FRET between pyrene moieties. The DNA scaffold was modified with two pyrenes and a quencher, anthraquinone. Homo-FRET was detected by monitoring quenching of pyrene emission and the decrease in the fluorescence lifetime of pyrene. Homo-FRET efficiencies could be calculated by excluding effects of hetero-FRET. The experimentally determined efficiencies showed an excellent agreement with Förster theory. These results will inform design of novel molecular probes and light-harvesting antennae.

DOI： 10.1002/cptc.202000199

Web of Science

Scopus

A Pyrene-Modified Serinol Nucleic Acid Nanostructure Converts the Chirality of Threoninol Nucleic Acids into Circularly Polarized Luminescence Signals 査読有り

Kashida H., Nishikawa K., Ito Y., Murayama K., Hayashi I., Kakuta T., Ogoshi T., Asanuma H.

Chemistry - A European Journal 2021年

担当区分：最終著者,　責任著者記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Chemistry - A European Journal

Herein is reported a circularly polarized luminescent (CPL) probe that can respond to the chirality of nucleic acids. An achiral nanostructure was prepared by the hybridization of symmetric serinol nucleic acid (SNA) containing pyrene-modified residues. When chiral oligomers that were complementary to the SNA were added, they induced helicity into the SNA nanowire. Efficient circular dichroism (CD) signal amplification was observed when pyrene was attached to uracil bases through a rigid alkynyl linker. Both CPL and CD signals were observed; they depended on the chirality of the added acyclic threoninol nucleic acid (aTNA) oligomer. This system can be used to convert the chirality of chiral biomolecules into chiroptical signals.

DOI： 10.1002/chem.202102333

Scopus

PubMed

RNA以前に非環状型XNAがPre-RNAとして存在したか？招待有り査読有り

浅沼浩之, 沖田ひかり, 村山恵司

Viva Origino 49 巻 ( 3 ) 頁： 9 2021年

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