-
A Cost-Effective Immobilization Method for MBP Fusion Proteins on Microtiter Plates Using a Gelatinized Starch-Agarose Mixture and Its Application for Convenient Protein-Protein Interaction Analysis
Reviewed
International journal
METHODS AND PROTOCOLS
Vol.
6
(
3
)
2023.6
-
Pharmacologic Comparison of High-Dose Hesperetin and Quercetin on MDCK II Cell Viability, Tight Junction Integrity, and Cell Shape
Reviewed
International journal
Nakashima, M; Goda, N; Tenno, T; Kotake, A; Inotsume, Y; Amaya, M; Hiroaki, H
ANTIOXIDANTS
Vol.
12
(
4
)
2023.4
-
Small-Molecule-Mediated Suppression of BMP Signaling by Selective Inhibition of BMP1-Dependent Chordin Cleavage
Reviewed
International journal
Mizoguchi Takamasa, Mikami Shohei, Yatou Mari, Kondo Yui, Omaru Shuhei, Kuwabara Shuhei, Okura Wataru, Noda Syouta, Tenno Takeshi, Hiroaki Hidekazu, Itoh Motoyuki
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Vol.
24
(
5
)
2023.3
-
Distinct motifs in the E protein are required for SARS-CoV-2 virus particle formation and lysosomal deacidification in host cells
Reviewed
International journal
Miura, K; Suzuki, Y; Ishida, K; Arakawa, M; Wu, H; Fujioka, Y; Emi, A; Maeda, K; Hamajima, R; Nakano, T; Tenno, T; Hiroaki, H; Morita, E
JOURNAL OF VIROLOGY
Vol.
97
(
10
)
page：
e0042623
2023.10
-
Molecular mechanisms of amyloid-beta peptide fibril and oligomer formation: NMR-based challenges
Invited
Reviewed
International journal
BIOPHYSICS AND PHYSICOBIOLOGY
Vol.
20
(
1
)
page：
e200007
2023
-
Relevance of Amorphous and Amyloid-Like Aggregates of the p53 Core Domain to Loss of its DNA-Binding Activity
Reviewed
International journal
Hibino Emi, Tenno Takeshi, Hiroaki Hidekazu
FRONTIERS IN MOLECULAR BIOSCIENCES
Vol.
9
page：
869851
2022.4
-
Direct Inhibition of the First PDZ Domain of ZO-1 by Glycyrrhizin is a Possible Mechanism of Tight Junction Opening of Caco-2 Cells.
Reviewed
International journal
Hibino, E., Goda, N., Hisada, M., Tenno, T., Hiroaki, H.
RSC Food & Function
Vol.
13
(
4
)
page：
1953
-
1964
2022.2
-
Potential of rescue and reactivation of tumor suppressor p53 for cancer therapy.
Invited
Reviewed
International journal
Hibino E, Hiroaki H
Biophysical reviews
Vol.
14
(
1
)
page：
267
-
275
2022.2
-
A cryptic phosphate-binding pocket on the SPFH domain of human stomatin that regulates a novel fibril-like self-assembly
Reviewed
International journal
Kataoka, K; Suzuki, S; Tenno, T; Goda, N; Hibino, E; Oshima, A; Hiroaki, H
CURRENT RESEARCH IN STRUCTURAL BIOLOGY
Vol.
4
page：
158
-
166
2022
-
Cryoprotective activities of FK20, a human genome-derived intrinsically disordered peptide against cryosensitive enzymes without a stereospecific molecular interaction
Reviewed
International journal
Naoki Matsuo, Natsuko Goda, Takeshi Tenno, and Hidekazu Hiroaki
PeerJ Physical Chemistry
Vol.
3
page：
e20
2021.12
-
Structural and Functional Analysis of Glycyrrhizin Bound to the Scaffold Protein ZO1-PDZ1 of Tight Junctions
Reviewed
International journal
Vol.
30
page：
119
-
119
2021.10
-
NMR-Guided Repositioning of Non-Steroidal Anti-Inflammatory Drugs into Tight Junction Modulators
Reviewed
International journal
Tenno T, Kataoka K, Goda N, Hiroaki H
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Vol.
22
(
5
)
2021.3
-
High dose of baicalin or baicalein can reduce tight junction integrity by partly targeting the first PDZ domain of zonula occludens-1 (ZO-1)
Reviewed
Hisada Misaki, Hiranuma Minami, Nakashima Mio, Goda Natsuko, Tenno Takeshi, Hiroaki Hidekazu
EUROPEAN JOURNAL OF PHARMACOLOGY
Vol.
887
page：
173436
2020.11
-
Opposing Effect of Naringenin and Quercetin on the Junctional Compartment of MDCK II Cells to Modulate the Tight Junction
Reviewed
International journal
Nakashima Mio, Hisada Misaki, Goda Natsuko, Tenno Takeshi, Kotake Ayaka, Inotsume Yuko, Kameoka Ikuo, Hiroaki Hidekazu
NUTRIENTS
Vol.
12
(
11
)
2020.11
-
Principal component analysis of data from NMR titration experiment of uniformly <sup>15</sup>N labeled amyloid beta (1-42) peptide with osmolytes and phenolic compounds.
Reviewed
Iwaya N, Goda N, Matsuzaki M, Narita A, Shigemitsu Y, Tenno T, Abe Y, Hoshi M, Hiroaki H
Archives of biochemistry and biophysics
Vol.
690
page：
108446
2020.9
-
Presence of intrinsically disordered proteins can inhibit the nucleation phase of amyloid fibril formation of A beta(1-42) in amino acid sequence independent manner
Reviewed
Ikeda Koki, Suzuki Shota, Shigemitsu Yoshiki, Tenno Takeshi, Goda Natsuko, Oshima Atsunori, Hiroaki Hidekazu
SCIENTIFIC REPORTS
Vol.
10
(
1
)
page：
12334
2020.7
-
Spatial Overlap of Claudin- and Phosphatidylinositol Phosphate-Binding Sites on the First PDZ Domain of Zonula Occludens 1 Studied by NMR.
Reviewed
Hiroaki H, Satomura K, Goda N, Nakakura Y, Hiranuma M, Tenno T, Hamada D, Ikegami T
Molecules (Basel, Switzerland)
Vol.
23
(
10
)
2018.10
-
Using H-1(N) amide temperature coefficients to define intrinsically disordered regions: An alternative NMR method
Reviewed
Okazaki Hiroki, Matsuo Naoki, Tenno Takeshi, Goda Natsuko, Shigemitsu Yoshiki, Ota Motonori, Hiroaki Hidekazu
PROTEIN SCIENCE
Vol.
27
(
10
)
page：
1821
-
1830
2018.10
-
Discovery of Potent Disheveled/Dvl Inhibitors Using Virtual Screening Optimized With NMR-Based Docking Performance Index
Invited
Reviewed
International journal
Hori Kiminori, Ajioka Kasumi, Goda Natsuko, Shindo Asako, Takagishi Maki, Tenno Takeshi, Hiroaki Hidekazu
FRONTIERS IN PHARMACOLOGY
Vol.
9
page：
983
2018.9
-
Analysis and comparison of amorphous& amyloid aggregates of the tumor suppressor p53
Hibino, E; Tenno, T; Hiroaki, H
BIOPHYSICAL JOURNAL
Vol.
122
(
3
)
page：
351A
-
351A
2023.2
-
Synthesis of selenomethylene-locked nucleic acids (SeLNA) nucleoside unit bearing an adenine base
Reviewed
International journal
Moai, Y; Hiroaki, H; Obika, S; Kodama, T
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
Vol.
39
(
1-3
)
page：
131
-
140
2020.2
-
Discovery of Cryoprotective Activity in Human Genome-Derived Intrinsically Disordered Proteins.
Matsuo N, Goda N, Shimizu K, Fukuchi S, Ota M, Hiroaki H
International journal of molecular sciences
Vol.
19
(
2
)
2018.2
-
Common molecular pathogenesis of disease-related intrinsically disordered proteins revealed by NMR analysis.
Reviewed
Shigemitsu Y, Hiroaki H
Journal of biochemistry
Vol.
163
(
1
)
page：
11-18
2018.1
-
Ecl1 is a zinc-binding protein involved in the zinc-limitation-dependent extension of chronological life span in fission yeast.
Reviewed
Shimasaki T, Ohtsuka H, Naito C, Azuma K, Tenno T, Hiroaki H, Murakami H, Aiba H
Molecular genetics and genomics : MGG
Vol.
292
(
2
)
page：
475-481
2017.4
-
Benzene-Glycol Nucleic Acid (BGNA)-DNA Chimeras: Synthesis, Binding Properties, and Ability to Elicit Human RNase H Activity.
Reviewed
Niwa, N., Shimizu, S., Maeda, Y., Hiroaki, H., Ueno, Y.
Vol.
7
page：
25378-25386
2017.1
-
Nuclear magnetic resonance evidence for the dimer formation of beta amyloid peptide 1-42 in 1,1,1,3,3,3-hexafluoro-2-propanol.
Reviewed
Shigemitsu Y, Iwaya N, Goda N, Matsuzaki M, Tenno T, Narita A, Hoshi M, Hiroaki H
Analytical biochemistry
Vol.
498
page：
59-67
2016.4
-
Na, K-ATPase α3 is a death target of Alzheimer patient amyloid-β assembly.
Reviewed
Vol.
112
(
32
)
page：
E4465-74
2015.8
-
用語解説 「天然変性蛋白質」
Invited
廣明秀一
再生医療（日本再生医療学会雑誌）
Vol.
14
(
3
)
page：
52-53
2015.8
-
A Method for Systematic Assessment of Intrinsically Disordered Protein Regions by NMR.
Reviewed
Goda N, Shimizu K, Kuwahara Y, Tenno T, Noguchi T, Ikegami T, Ota M, Hiroaki H
International journal of molecular sciences
Vol.
16
(
7
)
page：
15743-60
2015.7
-
Crystal structure of afadin PDZ domain-nectin-3 complex shows the structural plasticity of the ligand-binding site.
Reviewed
Fujiwara Y, Goda N, Tamashiro T, Narita H, Satomura K, Tenno T, Nakagawa A, Oda M, Suzuki M, Sakisaka T, Takai Y, Hiroaki H
Protein science : a publication of the Protein Society
Vol.
24
(
3
)
page：
376-85
2015.3
-
An optimized Npro-based method for the expression and purification of intrinsically disordered proteins for an NMR study
Reviewed
Natsuko Goda, Naoki Matsuo, Takeshi Tenno, Sonoko Ishino, Yoshizumi Ishino, Satoshi Fukuchi, Motonori Ota & Hidekazu Hiroaki*
ntrinsically Disordered Proteins
Vol.
3
(
1
)
page：
1-6
2015.2
-
Decreased amyloidogenicity caused by mutational modulation of surface properties of the immunoglobulin light chain BRE variable domain.
Reviewed
Kobayashi Y, Tsutsumi H, Abe T, Ikeda K, Tashiro Y, Unzai S, Kamikubo H, Kataoka M, Hiroaki H, Hamada D.
Biochemistry
Vol.
53
(
31
)
page：
5162-5173
2014.8
-
Multiple interactions of the intrinsically disordered region between the helicase and nuclease domains of the archaeal Hef protein.
Reviewed
Ishino S, Yamagami T, Kitamura M, Kodera N, Mori T, Sugiyama S, Ando T, Goda N, Tenno T, Hiroaki H, Ishino Y.
J Biol Chem.
Vol.
289
(
31
)
page：
21627-21639
2014.8
-
IDEAL in 2014 illustrates interaction networks composed of intrinsically disordered proteins and their binding partners.
Reviewed
Fukuchi S, Amemiya T, Sakamoto S, Nobe Y, Hosoda K, Kado Y, Murakami SD, Koike R, Hiroaki H, Ota M.
Nucleic Acids Res.
Vol.
42
(
Database issue
)
page：
D320-325
2014.1
-
特集「タンパク質構造機能相関再考」~ NMRで見るタンパク質－低分子相互作用 (2013)
Invited
廣明秀一
生化学
Vol.
85
(
8
)
page：
679-686
2013.8
-
Accidental Interaction between PDZ Domains and Diclofenac Revealed by NMR-Assisted Virtual Screening.
Reviewed
Tenno T, Goda N, Umetsu Y, Ota M, Kinoshita K, Hiroaki H.
Molecules
Vol.
18
(
8
)
page：
9567-9581
2013.8
-
Recent applications of isotopic labeling for protein NMR in drug discovery.
Invited
Reviewed
Hiroaki, H*
Expert Opinion on Drug Discovery
Vol.
8
(
5
)
page：
523-536
2013.3
-
Effect of Ca(2+) on the microtubule-severing enzyme p60-katanin. Insight into the substrate-dependent activation mechanism.
Reviewed
Iwaya N, Akiyama K, Goda N, Tenno T, Fujiwara Y, Hamada D, Ikura T, Shirakawa M, Hiroaki H.
FEBS J.
Vol.
279
(
7
)
page：
1339-1352.
2012.3
-
IDEAL: Intrinsically Disordered proteins with Extensive Annotations and Literature.
Reviewed
Fukuchi S, Sakamoto S, Nobe Y, Murakami SD, Amemiya T, Hosoda K, Koike R, Hiroaki H, Ota M.
Nucleic Acids Res.
Vol.
40
page：
D507-511.
2011.11
-
A simplified recipe for assigning amide NMR signals using combinatorial 14N amino acid inverse-labeling.
Reviewed
Hiroaki H, Umetsu Y, Nabeshima Y, Hoshi M, Kohda D.
J Struct Funct Genomics.
Vol.
12
(
3
)
page：
167-174
2011.8
-
Structure and Function of the N-terminal Nucleolin Binding Domain of Nuclear Valosin-containing Protein-like 2 (NVL2) Harboring a Nucleolar Localization Signal.
Reviewed
Fujiwara, Y., Fujiwara, K., Goda, N., Iwaya, N., Tenno, T., Shirakawa, M., and Hiroaki, H.
J Biol Chem.
Vol.
286
(
24
)
page：
21732-21741.
2011.6
-
(1)H, (13)C, and (15)N resonance assignment of the SPFH domain of human stomatin.
Reviewed
Tsuruta, T., Goda, N., Umetsu, Y., Iwaya, N., Kuwahara, Y., and Hiroaki, H.
Biomol NMR Assign.
Vol.
6
(
1
)
page：
23-25.
2011.6
-
SH3YL1 regulates dorsal ruffle formation by a novel phosphoinositide-binding domain.
Reviewed
Hasegawa, J., Tokuda, E., Tenno, T., Tsujita, K., Sawai, H., Hiroaki, H., Takenawa, T., and Itoh, T.
J Cell Biol.
Vol.
193
(
5
)
page：
901-916.
2011.5
-
Structural difference of vasoactive intestinal peptide in two distinct membrane-mimicking environments.
Reviewed
Umetsu, Y., Tenno, T., Goda, N., Shirakawa, M., Ikegami, T., and Hiroaki, H.
Biochim Biophys Acta.
Vol.
1814
(
5
)
page：
724-730.
2011.5
-
(1)H, (13)C, and (15)N resonance assignment of the first PDZ domain of mouse ZO-1.
Reviewed
Umetsu, Y., Goda, N., Taniguchi, R., Satomura, K., Ikegami, T., Furuse, M., and Hiroaki, H.
Biomol NMR Assign.
Vol.
5
(
2
)
page：
207-210.
2011.3
-
SAHG, a comprehensive database of predicted structures of all human proteins.
Reviewed
Motono, C., Nakata, J., Koike, R., Shimizu, K., Shirota, M., Amemiya, T., Tomii, K., Nagano, N., Sakaya, N., Misoo, K., Sato, M., Kidera, A., Hiroaki, H., Shirai, T., Kinoshita, K., Noguchi, T., and Ota, M.
Nucleic Acids Res.
Vol.
39
page：
D487-493.
2011.1
-
Crystal structure of a PFU-PUL domain pair of Saccharomyces cerevisiae Doa1/Ufd3.
Reviewed
Nishimasu, R., Komori, H., Higuchi, Y., Nishimasu, H., and Hiroaki, H.
Kobe J Med Sci.
Vol.
56
(
3
)
page：
E125-139.
2010.10
-
1H, 13C, and 15N resonance assignment of the TIR domain of human MyD88.
Reviewed
Ohnishi, H., Tochio, H., Kato, Z., Kimura, T., Hiroaki, H., Kondo, N., and Shirakawa, M.
Biomol NMR Assign.
Vol.
4
(
2
)
page：
123-125.
2010.10
-
A common substrate recognition mode conserved between katanin p60 and VPS4 governs microtubule severing and membrane skeleton reorganization.
Reviewed
Iwaya, N., Kuwahara, Y., Fujiwara, Y., Goda, N., Tenno, T., Akiyama, K., Mase, S., Tochio, H., Ikegami, T., Shirakawa, M., and Hiroaki, H.
J Biol Chem.
Vol.
285
(
22
)
page：
16822-16829.
2010.5
-
Structure and mutagenesis studies of the C-terminal region of licensing factor Cdt1 enable the identification of key residues for binding to replicative helicase Mcm proteins.
Reviewed
Jee, J., Mizuno, T., Kamada, K., Tochio, H., Chiba, Y., Yanagi, K., Yasuda, G., Hiroaki, H., Hanaoka, F., and Shirakawa, M.
J Biol Chem.
Vol.
285
(
21
)
page：
15931-15940.
2010.5
-
Unusual thermal disassembly of the SPFH domain oligomer from Pyrococcus horikoshii.
Reviewed
Kuwahara, Y., Unzai, S., Nagata, T., Hiroaki, Y., Yokoyama, H., Matsui, I., Ikegami, T., Fujiyoshi, Y., and Hiroaki, H.
Biophys J.
Vol.
97
(
7
)
page：
2034-2043.
2009.10
-
Structural basis for the multiple interactions of the MyD88 TIR domain in TLR4 signaling.
Reviewed
Ohnishi, H., Tochio, H., Kato, Z., Orii, K. E., Li, A., Kimura, T., Hiroaki, H., Kondo, N., and Shirakawa, M.
Proc Natl Acad Sci U S A.
Vol.
106
(
25
)
page：
10260-10265.
2009.6
-
High-resolution multi-dimensional NMR spectroscopy of proteins in human cells.
Reviewed
Inomata, K., Ohno, A., Tochio, H., Isogai, S., Tenno, T., Nakase, I., Takeuchi, T., Futaki, S., Ito, Y., Hiroaki, H., and Shirakawa, M.
Nature.
Vol.
458
(
7234
)
page：
106-109.
2009.3
-
The solution structure of the C-terminal domain of NfeD reveals a novel membrane-anchored OB-fold.
Reviewed
Kuwahara, Y., Ohno, A., Morii, T., Yokoyama, H., Matsui, I., Tochio, H., Shirakawa, M., and Hiroaki, H.
Protein Sci.
Vol.
17
(
11
)
page：
1915-1924.
2008.11
-
Intracellular protein delivery activity of peptides derived from insulin-like growth factor binding proteins 3 and 5.
Reviewed
Goda, N., Tenno, T., Inomata, K., Shirakawa, M., Tanaka, T., and Hiroaki, H.
Exp Cell Res.
Vol.
314
(
13
)
page：
2352-2361.
2008.8
-
DNA damage-induced ubiquitylation of RFC2 subunit of replication factor C complex.
Reviewed
Tomida, J., Masuda, Y., Hiroaki, H., Ishikawa, T., Song, I., Tsurimoto, T., Tateishi, S., Shiomi, T., Kamei, Y., Kim, J., Kamiya, K., Vaziri, C., Ohmori, H., and Todo, T.
J Biol Chem.
Vol.
283
(
14
)
page：
9071-9079.
2008.4
-
Kinetic and Thermodynamic Evidence for Flipping of a Methyl-CpG Binding Domain on Methylated DNA.
Reviewed
Inomata, K., Ohki, I., Tochio, H., Fujiwara, K., Hiroaki, H., and Shirakawa, M.
Biochemistry.
Vol.
47
(
10
)
page：
3266-3271.
2008.3
-
Fluoroscopic assessment of protein leakage during Xenopus oocytes in-cell NMR experiment by co-injected EGFP.
Reviewed
Sakai, T., Tochio, H., Inomata, K., Sasaki, Y., Tenno, T., Tanaka, T., Kokubo, T., Hiroaki, H., and Shirakawa, M.
Anal Biochem.
Vol.
371
(
2
)
page：
247-249.
2007.12
-
LBT/PTD dual tagged vector for purification, cellular protein delivery and visualization in living cells.
Reviewed
Goda, N., Tenno, T., Inomata, K., Iwaya, N., Sasaki, Y., Shirakawa, M., and Hiroaki, H.
Biochim Biophys Acta.
Vol.
1773
(
2
)
page：
141-146.
2007.2
-
Fine-tuning of protein domain boundary by minimizing potential coiled coil regions.
Reviewed
Iwaya, N., Goda, N., Unzai, S., Fujiwara, K., Tanaka, T., Tomii, K., Tochio, H., Shirakawa, M., and Hiroaki, H.
J Biomol NMR.
Vol.
37
(
1
)
page：
53-63.
2007.1
-
The common phospholipid-binding activity of the N-terminal domains of PEX1 and VCP/p97.
Reviewed
Shiozawa, K., Goda, N., Shimizu, T., Mizuguchi, K., Kondo, N., Shimozawa, N., Shirakawa, M., and Hiroaki, H.
FEBS J.
Vol.
273
(
21
)
page：
4959-4571.
2006.11
-
In-cell NMR spectroscopy of proteins inside Xenopus laevis oocytes.
Reviewed
Sakai, T., Tochio, H., Tenno, T., Ito, Y., Kokubo, T., Hiroaki, H., and Shirakawa, M.
J Biomol NMR.
Vol.
36
(
3
)
page：
179-188.
2006.11
-
Crystal structure of SUMO-3-modified thymine-DNA glycosylase.
Reviewed
Baba, D., Maita, N., Jee, J. G., Uchimura, Y., Saitoh, H., Sugasawa, K., Hanaoka, F., Tochio, H., Hiroaki, H., and Shirakawa, M.
J Mol Biol.
Vol.
359
(
1
)
page：
137-147.
2006.5
-
Optimization of size complementary of the aromatic side chain in the hydrophobic core of the designed coiled-coil.
Reviewed
Sakurai, Y., Mizuno, T., Hiroaki, H., Oku, J., and Tanaka, T.
J Pept Res.
Vol.
66
(
6
)
page：
387-394.
2005.12
-
Crystal structure of thymine DNA glycosylase conjugated to SUMO-1.
Reviewed
Baba, D., Maita, N., Jee, J. G., Uchimura, Y., Saitoh, H., Sugasawa, K., Hanaoka, F., Tochio, H., Hiroaki, H., and Shirakawa, M.
Nature.
Vol.
435
(
7044
)
page：
979-982.
2005.10
-
High thermal stability imparted by a designed tandem Arg-Trp stretch in an alpha-helical coiled coil.
Reviewed
Sakurai, Y., Mizuno, T., Hiroaki, H., Gohda, K., Oku, J., and Tanaka, T.
Angew Chem Int Ed Engl.
Vol.
44
(
38
)
page：
6180-6183.
2005.9
-
Structural characterization of the MIT domain from human Vps4b.
Reviewed
Takasu, H., Jee, J. G., Ohno, A., Goda, N., Fujiwara, K., Tochio, H., Shirakawa, M., and Hiroaki, H.
Biochem Biophys Res Commun.
Vol.
334
(
2
)
page：
460-465.
2005.8
-
Structure of the UBA domain of Dsk2p in complex with ubiquitin molecular determinants for ubiquitin recognition.
Reviewed
Ohno, A., Jee, J., Fujiwara, K., Tenno, T., Goda, N., Tochio, H., Kobayashi, H., Hiroaki, H., and Shirakawa, M.
Structure (Camb).
Vol.
13
(
4
)
page：
521-532.
2005.4
-
[High-throughput construction of expression vector for protein domain analysis].
Tenno, T., Goda, N., and Hiroaki, H.
Tanpakushitsu Kakusan Koso.
Vol.
49
(
16
)
page：
2587-2594.
2004.12
-
Structure of the N-terminal domain of PEX1 AAA-ATPase. Characterization of a putative adaptor-binding domain.
Reviewed
Shiozawa, K., Maita, N., Tomii, K., Seto, A., Goda, N., Akiyama, Y., Shimizu, T., Shirakawa, M., and Hiroaki, H.
J Biol Chem.
Vol.
279
(
48
)
page：
50060-50068.
2004.11
-
Crystallographic characterization of the N-terminal domain of PEX1.
Reviewed
Shiozawa, K., Maita, N., Tomii, K., Seto, A., Goda, N., Akiyama, Y., Shimizu, T., Shirakawa, M., and Hiroaki, H.
Acta Crystallogr D Biol Crystallogr.
Vol.
60
(
11
)
page：
2098-2099.
2004.11
-
Two-Metal Ion, Ni(II) and Cu(II), Binding alpha-Helical Coiled Coil Peptide.
Reviewed
Tanaka, T., Mizuno, T., Fukui, S., Hiroaki, H., Oku, J. I., Kanaori, K., Tajima, K., and Shirakawa, M.
J Am Chem Soc.
Vol.
126
(
43
)
page：
14023-14028.
2004.10
-
Structural basis for distinct roles of Lys63- and Lys48-linked polyubiquitin chains.
Reviewed
Tenno, T., Fujiwara, K., Tochio, H., Iwai, K., Morita, E. H., Hayashi, H., Murata, S., Hiroaki, H., Sato, M., Tanaka, K., and Shirakawa, M.
Genes Cells.
Vol.
9
(
10
)
page：
865-875.
2004.10
-
High-throughput construction method for expression vector of peptides for NMR study suited for isotopic labeling.
Reviewed
Tenno, T., Goda, N., Tateishi, Y., Tochio, H., Mishima, M., Hayashi, H., Shirakawa, M., and Hiroaki, H.
Protein Eng Des Sel.
Vol.
17
(
4
)
page：
305-314.
2004.4
-
The PRESAT-vector: asymmetric T-vector for high-throughput screening of soluble protein domains for structural proteomics.
Reviewed
Goda, N., Tenno, T., Takasu, H., Hiroaki, H., and Shirakawa, M.
Protein Sci.
Vol.
13
(
3
)
page：
652-658.
2004.3
-
Structure of the ubiquitin-interacting motif of S5a bound to the ubiquitin-like domain of HR23B.
Reviewed
Fujiwara, K., Tenno, T., Sugasawa, K., Jee, J. G., Ohki, I., Kojima, C., Tochio, H., Hiroaki, H., Hanaoka, F., and Shirakawa, M.
J Biol Chem.
Vol.
279
(
6
)
page：
4760-4767.
2004.2
-
Thermodynamic study of hybridization properties of heterochiral nucleic acids.
Reviewed
Urata, H., Shimizu, H., Hiroaki, H., Kohda, D., and Akagi, M.
Biochem Biophys Res Commun.
Vol.
309
(
1
)
page：
79-83.
2003.9
-
1H, 13C and 15N backbone resonance assignments of the N-terminal domain of Drosophila GCM protein.
Reviewed
Shimizu, M., Hiroaki, H., Kohda, D., Morita, E. H., Hotta, S., and Morikawa, K.
J Biomol NMR.
Vol.
26
(
3
)
page：
277-278.
2003.7
-
Phosphorylation of p47phox directs phox homology domain from SH3 domain toward phosphoinositides, leading to phagocyte NADPH oxidase activation.
Reviewed
Ago, T., Kuribayashi, F., Hiroaki, H., Takeya, R., Ito, T., Kohda, D., and Sumimoto, H.
Proc Natl Acad Sci U S A.
Vol.
100
(
8
)
page：
4474-4479.
2003.4
-
NMR and ICP spectroscopic analysis of the DNA-binding domain of the Drosophila GCM protein reveals a novel Zn2+ -binding motif.
Reviewed
Shimizu, M., Hiroaki, H., Kohda, D., Hosoya, T., Akiyama-Oda, Y., Hotta, Y., Morita, E. H., and Morikawa, K.
Protein Eng.
Vol.
16
(
4
)
page：
247-254.
2003.4
-
[Membrane associating protein domains: a new paradigm of phosphoinositide binding domains].
Hiroaki, H.
Tanpakushitsu Kakusan Koso.
Vol.
46
(
13
)
page：
1936-1942.
2001.10
-
The PX domain as a novel phosphoinositide- binding module.
Reviewed
Ago, T., Takeya, R., Hiroaki, H., Kuribayashi, F., Ito, T., Kohda, D., and Sumimoto, H.
Biochem Biophys Res Commun.
Vol.
287
(
3
)
page：
733-738.
2001.9
-
Solution structure of the PX domain, a target of the SH3 domain.
Reviewed
Hiroaki, H., Ago, T., Ito, T., Sumimoto, H., and Kohda, D.
Nat Struct Biol.
Vol.
8
(
6
)
page：
526-530.
2001.6
-
Characterization of DNA, RNA and DNA/RNA duplexes containing an L-nucleotide.
Urata, H., Shimizu, H., Hiroaki, H., Kohda, D., and Akagi, M.
Nucleic Acids Res Suppl.
Vol.
1
page：
243-244.
2001
-
Design of a Heterotrimeric alpha -Helical Bundle by Hydrophobic Core Engineering.
Reviewed
Kashiwada, A., Hiroaki, H., Kohda, D., Nango, M., and Tanaka, T.
J Am Chem Soc.
Vol.
122
(
2
)
page：
212 -215.
2000.12
-
Soft metal ions, Cd(II) and Hg(II), induce triple-stranded alpha-helical assembly and folding of a de novo designed peptide in their trigonal geometries.
Reviewed
Li, X., Suzuki, K., Kanaori, K., Tajima, K., Kashiwada, A., Hiroaki, H., Kohda, D., and Tanaka, T.
Protein Sci.
Vol.
9
(
7
)
page：
1327-1333.
2000.7
-
A'-form RNA double helix in the single crystal structure of r(UGAGCUUCGGCUC).
Reviewed
Tanaka, Y., Fujii, S., Hiroaki, H., Sakata, T., Tanaka, T., Uesugi, S., Tomita, K., and Kyogoku, Y.
Nucleic Acids Res.
Vol.
27
(
4
)
page：
949-955.
1999.2
-
Influence of various nucleotides on the in situ crystallization of Ca2+-ATPase.
Reviewed
Hiroaki, Y., Mitsuoka, K., Cheng, Y., Hiroaki, H., and Fujiyoshi, Y.
Biochim Biophys Acta.
Vol.
1415
(
2
)
page：
361-368.
1999.1
-
Metal Ion Induced Self-Assembly of a Designed Peptide into a Triple-Stranded a Helical Bundle: A Novel Metal Binding Site in the Hydrophobic Core.
Reviewed
Suzuki, K., Hiroaki, H., Kohda, D., Nakamura, H., and Tanaka, T.
J Am Chem Soc.
Vol.
120
(
50
)
page：
13008 -13015.
1998.12
-
An isoleucine zipper peptide forms a native-like triple stranded coiled coil in solution.
Reviewed
Suzuki, K., Hiroaki, H., Kohda, D., and Tanaka, T.
Protein Eng.
Vol.
11
(
11
)
page：
1051-1055.
1998.11
-
Determination of the solution structure of the SH3 domain of human p56 Lck tyrosine kinase.
Reviewed
Hiroaki, H., Klaus, W., and Senn, H.
J Biomol NMR.
Vol.
8
(
2
)
page：
105-122.
1996.9
-
Cloning of boar SPMI gene which is expressed specifically in seminal vesicle and codes for a sperm motility inhibitor protein.
Reviewed
Iwamoto, T., Hiroaki, H., Furuichi, Y., Wada, K., Satoh, M., Satoh, M., Osada, T., and Gagnon, C.
FEBS Lett.
Vol.
368
(
3
)
page：
420-424.
1995.7
-
Spectroscopic Characterization of the Decadeoxynucleotide Duplex Modified with Dichloroethylene-diaminoplatinum(II).
Reviewed
Urata, H., Urata, M., Akagi, M., Hiroaki, H., and Uesugi, S.
Nucleosides & Nucleotides.
Vol.
13
page：
1259-1269.
1994.7
-
Properties Of A Hammerhead-Type Rna Enzyme-System That Consists Of 3 Rna Oligomer Strands.
Reviewed
Odai, O., Hiroaki, H., Tanaka, T., and Uesugi, S.
Nucleosides & Nucleotides.
Vol.
13
page：
1569-1579.
1994.7
-
Properties of hammerhead-type RNA enzyme derivatives which contain a G-to-I replacement in the loop region.
Uesugi, S., Kodama, H., Hiroaki, H., and Odai, O.
Nucleic Acids Symp Ser.
Vol.
27
page：
13-14.
1992
-
Conformational features of the four successive non-Watson-Crick base pairs in RNA duplex.
Fujii, S., Tanaka, Y., Uesugi, S., Tanaka, T., Sakata, T., and Hiroaki, H.
Nucleic Acids Symp Ser.
Vol.
27
page：
63-64.
1992
-
Interaction of bleomycin with deoxyribonucleic acid oligomer: proton nuclear magnetic resonance titration study using novel bleomycin complexes with Ni2+ and VO3+.
Reviewed
Hiroaki, H., Nakayama, T., Ikehara, M., and Uesugi, S.
Chem Pharm Bull (Tokyo).
Vol.
39
(
11
)
page：
2780-2786.
1991.11
-
Conformation of 2'GMP bound to a mutant ribonuclease T1 (Y45W) determined by X-ray diffraction and NMR methods.
Reviewed
Itoh, T., Tomita, K., Hakoshima, T., Hiroaki, H., Uesugi, S., Nishikawa, S., Amisaki, T., Morioka, H., Ohtsuka, E., and Ikehara, M.
J Biochem (Tokyo).
Vol.
110
(
5
)
page：
677-680.
1991.11
-
The molecular recognition mechanism of DNA by bleomycin.
Hiroaki, H., Uesugi, S., and Fujii, S.
Nucleic Acids Symp Ser.
Vol.
25
page：
147-148.
1991
-
Molecular structure of extraordinarily stable RNA: model building and X-ray analysis.
Fujii, S., Tanaka, Y., Tomita, K., Sakata, T., Hiroaki, H., Tanaka, T., and Uesugi, S.
Nucleic Acids Symp Ser.
Vol.
25
page：
181-182.
1991
-
Synthesis and NMR study of ribo-oligonucleotides forming a hammerhead-type RNA enzyme system.
Reviewed
Odai, O., Kodama, H., Hiroaki, H., Sakata, T., Tanaka, T., and Uesugi, S.
Nucleic Acids Res.
Vol.
18
(
20
)
page：
5955-5960.
1990.10
-
Studies on the structure and stabilizing factor of the CUUCGG hairpin RNA using chemically synthesized oligonucleotides.
Reviewed
Sakata, T., Hiroaki, H., Oda, Y., Tanaka, T., Ikehara, M., and Uesugi, S.
Nucleic Acids Res.
Vol.
18
(
13
)
page：
3831-3839.
1990.7
-
The role of a conserved guanosine residue in the hammerhead-type RNA enzyme.
Reviewed
Odai, O., Hiroaki, H., Sakata, T., Tanaka, T., and Uesugi, S.
FEBS Lett.
Vol.
267
(
1
)
page：
150-152.
1990.7
-
Conformation and properties of hammerhead-type RNA enzymes.
Uesugi, S., Odai, O., Hiroaki, H., Kodama, H., Sakata, T., and Tanaka, T.
Nucleic Acids Symp Ser.
Vol.
22
page：
41-42.
1990
-
Base recognition mechanism of bleomycin: solution structure of d(GGGGAGCTCCCC)2 based on 1H-NMR and restrained molecular dynamics.
Hiroaki, H., Ebata, T., Uesugi, S., and Fujii, S.
Nucleic Acids Symp Ser.
Vol.
22
page：
53-54.
1990
-
Identification of DNA degradation products by antitumor antibiotic, esperamicin.
Ebata, T., Hiroaki, H., Uesugi, S., and Sugiura, Y.
Nucleic Acids Symp Ser.
Vol.
22
page：
19-20.
1990
-
A 2-deoxyribonolactone-containing nucleotide: isolation and characterization of the alkali-sensitive photoproduct of the trideoxyribonucleotide d(ApCpA).
Reviewed
Urata, H., Yamamoto, K., Akagi, M., Hiroaki, H., and Uesugi, S.
Biochemistry.
Vol.
28
(
25
)
page：
9566-9569.
1989.12
-
Novel 1H-31P heteronuclear coherence transfer spectroscopy based on spin locking MLEV-17 and its application to signal assignment for a short DNA fragment.
Reviewed
Hiroaki, H., and Uesugi, S.
FEBS Lett.
Vol.
244
(
1
)
page：
43-46.
1989.2
-
Synthesis and properties of RNA with catalytic activity.
Odai, O., Sakata, T., Orita, M., Hiroaki, H., Uesugi, S., and Tanaka, T.
Nucleic Acids Symp Ser.
Vol.
21
page：
105-106.
1989
-
NMR studies on DNA hairpin structures with a five nucleotide loop.
Uesugi, S., Oda, Y., Hiroaki, H., and Ikehara, M.
Nucleic Acids Symp Ser.
Vol.
21
page：
65-66.
1989
