-
A Cost-Effective Immobilization Method for MBP Fusion Proteins on Microtiter Plates Using a Gelatinized Starch-Agarose Mixture and Its Application for Convenient Protein-Protein Interaction Analysis
査読有り
国際誌
Emoto, Y; Katayama, R; Hibino, E; Ishihara, S; Goda, N; Tenno, T; Kobashigawa, Y; Morioka, H; Hiroaki, H
METHODS AND PROTOCOLS
6
巻
(
3
)
2023年6月
-
Pharmacologic Comparison of High-Dose Hesperetin and Quercetin on MDCK II Cell Viability, Tight Junction Integrity, and Cell Shape
査読有り
国際誌
Nakashima, M; Goda, N; Tenno, T; Kotake, A; Inotsume, Y; Amaya, M; Hiroaki, H
ANTIOXIDANTS
12
巻
(
4
)
2023年4月
-
Small-Molecule-Mediated Suppression of BMP Signaling by Selective Inhibition of BMP1-Dependent Chordin Cleavage
査読有り
国際誌
Mizoguchi Takamasa, Mikami Shohei, Yatou Mari, Kondo Yui, Omaru Shuhei, Kuwabara Shuhei, Okura Wataru, Noda Syouta, Tenno Takeshi, Hiroaki Hidekazu, Itoh Motoyuki
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
24
巻
(
5
)
2023年3月
-
Distinct motifs in the E protein are required for SARS-CoV-2 virus particle formation and lysosomal deacidification in host cells
査読有り
国際誌
Miura, K; Suzuki, Y; Ishida, K; Arakawa, M; Wu, H; Fujioka, Y; Emi, A; Maeda, K; Hamajima, R; Nakano, T; Tenno, T; Hiroaki, H; Morita, E
JOURNAL OF VIROLOGY
97
巻
(
10
)
頁：
e0042623
2023年10月
-
Molecular mechanisms of amyloid-beta peptide fibril and oligomer formation: NMR-based challenges
招待有り
査読有り
国際誌
Hiroaki, H
BIOPHYSICS AND PHYSICOBIOLOGY
20
巻
(
1
)
頁：
e200007
2023年
-
Relevance of Amorphous and Amyloid-Like Aggregates of the p53 Core Domain to Loss of its DNA-Binding Activity
査読有り
国際誌
Hibino Emi, Tenno Takeshi, Hiroaki Hidekazu
FRONTIERS IN MOLECULAR BIOSCIENCES
9
巻
頁：
869851
2022年4月
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Direct inhibition of the first PDZ domain of ZO-1 by glycyrrhizin is a possible mechanism of tight junction opening of Caco-2 cells
査読有り
国際誌
Hibino, E; Goda, N; Hisada, M; Tenno, T; Hiroaki, H
FOOD & FUNCTION
13
巻
(
4
)
頁：
1953
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1964
2022年2月
-
Potential of rescue and reactivation of tumor suppressor p53 for cancer therapy
招待有り
査読有り
国際誌
Hibino, E; Hiroaki, H
BIOPHYSICAL REVIEWS
14
巻
(
1
)
頁：
267
-
275
2022年2月
-
A cryptic phosphate-binding pocket on the SPFH domain of human stomatin that regulates a novel fibril-like self-assembly
査読有り
国際誌
Kataoka, K; Suzuki, S; Tenno, T; Goda, N; Hibino, E; Oshima, A; Hiroaki, H
CURRENT RESEARCH IN STRUCTURAL BIOLOGY
4
巻
頁：
158
-
166
2022年
-
Cryoprotective activities of FK20, a human genome-derived intrinsically disordered peptide against cryosensitive enzymes without a stereospecific molecular interaction
査読有り
国際誌
Naoki Matsuo, Natsuko Goda, Takeshi Tenno, and Hidekazu Hiroaki
PeerJ Physical Chemistry
3
巻
頁：
e20
2021年12月
-
1Structural and Functional Analysis of Glycyrrhizin Bound to the Scaffold Protein ZO1-PDZ1 of Tight Junctions
査読有り
国際誌
Hibino, E; Hisada, M; Goda, N; Tenno, T; Hiroaki, H
PROTEIN SCIENCE
30
巻
頁：
119
-
119
2021年10月
-
NMR-Guided Repositioning of Non-Steroidal Anti-Inflammatory Drugs into Tight Junction Modulators
査読有り
国際誌
Tenno, T; Kataoka, K; Goda, N; Hiroaki, H
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
22
巻
(
5
)
2021年3月
-
High dose of baicalin or baicalein can reduce tight junction integrity by partly targeting the first PDZ domain of zonula occludens-1 (ZO-1)
査読有り
Hisada, M; Hiranuma, M; Nakashima, M; Goda, N; Tenno, T; Hiroaki, H
EUROPEAN JOURNAL OF PHARMACOLOGY
887
巻
頁：
173436
2020年11月
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Opposing Effect of Naringenin and Quercetin on the Junctional Compartment of MDCK II Cells to Modulate the Tight Junction
査読有り
国際誌
Nakashima, M; Hisada, M; Goda, N; Tenno, T; Kotake, A; Inotsume, Y; Kameoka, I; Hiroaki, H
NUTRIENTS
12
巻
(
11
)
2020年11月
-
Principal component analysis of data from NMR titration experiment of uniformly N-15 labeled amyloid beta (1-42) peptide with osmolytes and phenolic compounds
査読有り
Iwaya Naoko, Goda Natsuko, Matsuzaki Mizuki, Narita Akihiro, Shigemitsu Yoshiki, Tenno Takeshi, Abe Yoshito, Hoshi Minako, Hiroaki Hidekazu
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
690
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頁：
108446
2020年9月
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Presence of intrinsically disordered proteins can inhibit the nucleation phase of amyloid fibril formation of A beta(1-42) in amino acid sequence independent manner
査読有り
Ikeda Koki, Suzuki Shota, Shigemitsu Yoshiki, Tenno Takeshi, Goda Natsuko, Oshima Atsunori, Hiroaki Hidekazu
SCIENTIFIC REPORTS
10
巻
(
1
)
頁：
12334
2020年7月
-
Spatial Overlap of Claudin- and Phosphatidylinositol Phosphate-Binding Sites on the First PDZ Domain of Zonula Occludens 1 Studied by NMR
査読有り
Hiroaki Hidekazu, Satomura Kaori, Goda Natsuko, Nakakura Yukako, Hiranuma Minami, Tenno Takeshi, Hamada Daizo, Ikegami Takahisa
MOLECULES
23
巻
(
10
)
2018年10月
-
Using H-1(N) amide temperature coefficients to define intrinsically disordered regions: An alternative NMR method
査読有り
Okazaki Hiroki, Matsuo Naoki, Tenno Takeshi, Goda Natsuko, Shigemitsu Yoshiki, Ota Motonori, Hiroaki Hidekazu
PROTEIN SCIENCE
27
巻
(
10
)
頁：
1821
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1830
2018年10月
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Discovery of Potent Disheveled/Dvl Inhibitors Using Virtual Screening Optimized With NMR-Based Docking Performance Index
招待有り
査読有り
国際誌
Hori, K; Ajioka, K; Goda, N; Shindo, A; Takagishi, M; Tenno, T; Hiroaki, H
FRONTIERS IN PHARMACOLOGY
9
巻
頁：
983
2018年9月
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Analysis and comparison of amorphous& amyloid aggregates of the tumor suppressor p53
Hibino, E; Tenno, T; Hiroaki, H
BIOPHYSICAL JOURNAL
122
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(
3
)
頁：
351A
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351A
2023年2月
-
Synthesis of selenomethylene-locked nucleic acids (SeLNA) nucleoside unit bearing an adenine base
査読有り
国際誌
Moai, Y; Hiroaki, H; Obika, S; Kodama, T
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
39
巻
(
1-3
)
頁：
131
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140
2020年2月
-
Discovery of Cryoprotective Activity in Human Genome-Derived Intrinsically Disordered Proteins
Matsuo Naoki, Goda Natsuko, Shimizu Kana, Fukuchi Satoshi, Ota Motonori, Hiroaki Hidekazu
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
19
巻
(
2
)
2018年2月
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Common molecular pathogenesis of disease-related intrinsically disordered proteins revealed by NMR analysis.
査読有り
Shigemitsu Y, Hiroaki H
Journal of biochemistry
163
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(
1
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頁：
11-18
2018年1月
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Ecl1 is a zinc-binding protein involved in the zinc-limitation-dependent extension of chronological life span in fission yeast.
査読有り
Shimasaki T, Ohtsuka H, Naito C, Azuma K, Tenno T, Hiroaki H, Murakami H, Aiba H
Molecular genetics and genomics : MGG
292
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(
2
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頁：
475-481
2017年4月
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Benzene-Glycol Nucleic Acid (BGNA)-DNA Chimeras: Synthesis, Binding Properties, and Ability to Elicit Human RNase H Activity.
査読有り
Niwa, N., Shimizu, S., Maeda, Y., Hiroaki, H., Ueno, Y.
7
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頁：
25378-25386
2017年1月
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Nuclear magnetic resonance evidence for the dimer formation of beta amyloid peptide 1-42 in 1,1,1,3,3,3-hexafluoro-2-propanol.
査読有り
Shigemitsu Y, Iwaya N, Goda N, Matsuzaki M, Tenno T, Narita A, Hoshi M, Hiroaki H
Analytical biochemistry
498
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59-67
2016年4月
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Na, K-ATPase α3 is a death target of Alzheimer patient amyloid-β assembly.
査読有り
112
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32
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E4465-74
2015年8月
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用語解説 「天然変性蛋白質」
招待有り
廣明秀一
再生医療（日本再生医療学会雑誌）
14
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(
3
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頁：
52-53
2015年8月
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A Method for Systematic Assessment of Intrinsically Disordered Protein Regions by NMR.
査読有り
Goda N, Shimizu K, Kuwahara Y, Tenno T, Noguchi T, Ikegami T, Ota M, Hiroaki H
International journal of molecular sciences
16
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7
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頁：
15743-60
2015年7月
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Crystal structure of afadin PDZ domain-nectin-3 complex shows the structural plasticity of the ligand-binding site.
査読有り
Fujiwara Y, Goda N, Tamashiro T, Narita H, Satomura K, Tenno T, Nakagawa A, Oda M, Suzuki M, Sakisaka T, Takai Y, Hiroaki H
Protein science : a publication of the Protein Society
24
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3
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頁：
376-85
2015年3月
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An optimized Npro-based method for the expression and purification of intrinsically disordered proteins for an NMR study
査読有り
Natsuko Goda, Naoki Matsuo, Takeshi Tenno, Sonoko Ishino, Yoshizumi Ishino, Satoshi Fukuchi, Motonori Ota & Hidekazu Hiroaki*
ntrinsically Disordered Proteins
3
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1
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頁：
1-6
2015年2月
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Decreased amyloidogenicity caused by mutational modulation of surface properties of the immunoglobulin light chain BRE variable domain.
査読有り
Kobayashi Y, Tsutsumi H, Abe T, Ikeda K, Tashiro Y, Unzai S, Kamikubo H, Kataoka M, Hiroaki H, Hamada D.
Biochemistry
53
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31
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5162-5173
2014年8月
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Multiple interactions of the intrinsically disordered region between the helicase and nuclease domains of the archaeal Hef protein.
査読有り
Ishino S, Yamagami T, Kitamura M, Kodera N, Mori T, Sugiyama S, Ando T, Goda N, Tenno T, Hiroaki H, Ishino Y.
J Biol Chem.
289
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31
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21627-21639
2014年8月
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IDEAL in 2014 illustrates interaction networks composed of intrinsically disordered proteins and their binding partners.
査読有り
Fukuchi S, Amemiya T, Sakamoto S, Nobe Y, Hosoda K, Kado Y, Murakami SD, Koike R, Hiroaki H, Ota M.
Nucleic Acids Res.
42
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D320-325
2014年1月
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特集「タンパク質構造機能相関再考」~ NMRで見るタンパク質－低分子相互作用 (2013)
招待有り
廣明秀一
生化学
85
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8
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679-686
2013年8月
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Accidental Interaction between PDZ Domains and Diclofenac Revealed by NMR-Assisted Virtual Screening.
査読有り
Tenno T, Goda N, Umetsu Y, Ota M, Kinoshita K, Hiroaki H.
Molecules
18
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8
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9567-9581
2013年8月
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Recent applications of isotopic labeling for protein NMR in drug discovery.
招待有り
査読有り
Hiroaki, H*
Expert Opinion on Drug Discovery
8
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5
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頁：
523-536
2013年3月
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Effect of Ca(2+) on the microtubule-severing enzyme p60-katanin. Insight into the substrate-dependent activation mechanism.
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Iwaya N, Akiyama K, Goda N, Tenno T, Fujiwara Y, Hamada D, Ikura T, Shirakawa M, Hiroaki H.
FEBS J.
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2012年3月
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IDEAL: Intrinsically Disordered proteins with Extensive Annotations and Literature.
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Fukuchi S, Sakamoto S, Nobe Y, Murakami SD, Amemiya T, Hosoda K, Koike R, Hiroaki H, Ota M.
Nucleic Acids Res.
40
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2011年11月
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A simplified recipe for assigning amide NMR signals using combinatorial 14N amino acid inverse-labeling.
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Hiroaki H, Umetsu Y, Nabeshima Y, Hoshi M, Kohda D.
J Struct Funct Genomics.
12
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3
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167-174
2011年8月
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Structure and Function of the N-terminal Nucleolin Binding Domain of Nuclear Valosin-containing Protein-like 2 (NVL2) Harboring a Nucleolar Localization Signal.
査読有り
Fujiwara, Y., Fujiwara, K., Goda, N., Iwaya, N., Tenno, T., Shirakawa, M., and Hiroaki, H.
J Biol Chem.
286
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2011年6月
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(1)H, (13)C, and (15)N resonance assignment of the SPFH domain of human stomatin.
査読有り
Tsuruta, T., Goda, N., Umetsu, Y., Iwaya, N., Kuwahara, Y., and Hiroaki, H.
Biomol NMR Assign.
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2011年6月
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SH3YL1 regulates dorsal ruffle formation by a novel phosphoinositide-binding domain.
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Hasegawa, J., Tokuda, E., Tenno, T., Tsujita, K., Sawai, H., Hiroaki, H., Takenawa, T., and Itoh, T.
J Cell Biol.
193
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2011年5月
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Structural difference of vasoactive intestinal peptide in two distinct membrane-mimicking environments.
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Umetsu, Y., Tenno, T., Goda, N., Shirakawa, M., Ikegami, T., and Hiroaki, H.
Biochim Biophys Acta.
1814
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2011年5月
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(1)H, (13)C, and (15)N resonance assignment of the first PDZ domain of mouse ZO-1.
査読有り
Umetsu, Y., Goda, N., Taniguchi, R., Satomura, K., Ikegami, T., Furuse, M., and Hiroaki, H.
Biomol NMR Assign.
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2
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2011年3月
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SAHG, a comprehensive database of predicted structures of all human proteins.
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Motono, C., Nakata, J., Koike, R., Shimizu, K., Shirota, M., Amemiya, T., Tomii, K., Nagano, N., Sakaya, N., Misoo, K., Sato, M., Kidera, A., Hiroaki, H., Shirai, T., Kinoshita, K., Noguchi, T., and Ota, M.
Nucleic Acids Res.
39
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2011年1月
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Crystal structure of a PFU-PUL domain pair of Saccharomyces cerevisiae Doa1/Ufd3.
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Nishimasu, R., Komori, H., Higuchi, Y., Nishimasu, H., and Hiroaki, H.
Kobe J Med Sci.
56
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3
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2010年10月
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1H, 13C, and 15N resonance assignment of the TIR domain of human MyD88.
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Ohnishi, H., Tochio, H., Kato, Z., Kimura, T., Hiroaki, H., Kondo, N., and Shirakawa, M.
Biomol NMR Assign.
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2
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2010年10月
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A common substrate recognition mode conserved between katanin p60 and VPS4 governs microtubule severing and membrane skeleton reorganization.
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Iwaya, N., Kuwahara, Y., Fujiwara, Y., Goda, N., Tenno, T., Akiyama, K., Mase, S., Tochio, H., Ikegami, T., Shirakawa, M., and Hiroaki, H.
J Biol Chem.
285
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2010年5月
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Structure and mutagenesis studies of the C-terminal region of licensing factor Cdt1 enable the identification of key residues for binding to replicative helicase Mcm proteins.
査読有り
Jee, J., Mizuno, T., Kamada, K., Tochio, H., Chiba, Y., Yanagi, K., Yasuda, G., Hiroaki, H., Hanaoka, F., and Shirakawa, M.
J Biol Chem.
285
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2010年5月
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Unusual thermal disassembly of the SPFH domain oligomer from Pyrococcus horikoshii.
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Kuwahara, Y., Unzai, S., Nagata, T., Hiroaki, Y., Yokoyama, H., Matsui, I., Ikegami, T., Fujiyoshi, Y., and Hiroaki, H.
Biophys J.
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2009年10月
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Structural basis for the multiple interactions of the MyD88 TIR domain in TLR4 signaling.
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Ohnishi, H., Tochio, H., Kato, Z., Orii, K. E., Li, A., Kimura, T., Hiroaki, H., Kondo, N., and Shirakawa, M.
Proc Natl Acad Sci U S A.
106
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2009年6月
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High-resolution multi-dimensional NMR spectroscopy of proteins in human cells.
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Inomata, K., Ohno, A., Tochio, H., Isogai, S., Tenno, T., Nakase, I., Takeuchi, T., Futaki, S., Ito, Y., Hiroaki, H., and Shirakawa, M.
Nature.
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2009年3月
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The solution structure of the C-terminal domain of NfeD reveals a novel membrane-anchored OB-fold.
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Kuwahara, Y., Ohno, A., Morii, T., Yokoyama, H., Matsui, I., Tochio, H., Shirakawa, M., and Hiroaki, H.
Protein Sci.
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2008年11月
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Intracellular protein delivery activity of peptides derived from insulin-like growth factor binding proteins 3 and 5.
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Goda, N., Tenno, T., Inomata, K., Shirakawa, M., Tanaka, T., and Hiroaki, H.
Exp Cell Res.
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2008年8月
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DNA damage-induced ubiquitylation of RFC2 subunit of replication factor C complex.
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Tomida, J., Masuda, Y., Hiroaki, H., Ishikawa, T., Song, I., Tsurimoto, T., Tateishi, S., Shiomi, T., Kamei, Y., Kim, J., Kamiya, K., Vaziri, C., Ohmori, H., and Todo, T.
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Kinetic and Thermodynamic Evidence for Flipping of a Methyl-CpG Binding Domain on Methylated DNA.
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Inomata, K., Ohki, I., Tochio, H., Fujiwara, K., Hiroaki, H., and Shirakawa, M.
Biochemistry.
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2008年3月
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Fluoroscopic assessment of protein leakage during Xenopus oocytes in-cell NMR experiment by co-injected EGFP.
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Sakai, T., Tochio, H., Inomata, K., Sasaki, Y., Tenno, T., Tanaka, T., Kokubo, T., Hiroaki, H., and Shirakawa, M.
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Goda, N., Tenno, T., Inomata, K., Iwaya, N., Sasaki, Y., Shirakawa, M., and Hiroaki, H.
Biochim Biophys Acta.
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2007年2月
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Fine-tuning of protein domain boundary by minimizing potential coiled coil regions.
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Iwaya, N., Goda, N., Unzai, S., Fujiwara, K., Tanaka, T., Tomii, K., Tochio, H., Shirakawa, M., and Hiroaki, H.
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The common phospholipid-binding activity of the N-terminal domains of PEX1 and VCP/p97.
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In-cell NMR spectroscopy of proteins inside Xenopus laevis oocytes.
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Sakai, T., Tochio, H., Tenno, T., Ito, Y., Kokubo, T., Hiroaki, H., and Shirakawa, M.
J Biomol NMR.
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Crystal structure of SUMO-3-modified thymine-DNA glycosylase.
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Baba, D., Maita, N., Jee, J. G., Uchimura, Y., Saitoh, H., Sugasawa, K., Hanaoka, F., Tochio, H., Hiroaki, H., and Shirakawa, M.
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Optimization of size complementary of the aromatic side chain in the hydrophobic core of the designed coiled-coil.
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J Pept Res.
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2005年12月
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Crystal structure of thymine DNA glycosylase conjugated to SUMO-1.
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Baba, D., Maita, N., Jee, J. G., Uchimura, Y., Saitoh, H., Sugasawa, K., Hanaoka, F., Tochio, H., Hiroaki, H., and Shirakawa, M.
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Sakurai, Y., Mizuno, T., Hiroaki, H., Gohda, K., Oku, J., and Tanaka, T.
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Structural characterization of the MIT domain from human Vps4b.
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Takasu, H., Jee, J. G., Ohno, A., Goda, N., Fujiwara, K., Tochio, H., Shirakawa, M., and Hiroaki, H.
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Structure of the UBA domain of Dsk2p in complex with ubiquitin molecular determinants for ubiquitin recognition.
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Ohno, A., Jee, J., Fujiwara, K., Tenno, T., Goda, N., Tochio, H., Kobayashi, H., Hiroaki, H., and Shirakawa, M.
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16
)
頁：
2587-2594.
2004年12月
-
Structure of the N-terminal domain of PEX1 AAA-ATPase. Characterization of a putative adaptor-binding domain.
査読有り
Shiozawa, K., Maita, N., Tomii, K., Seto, A., Goda, N., Akiyama, Y., Shimizu, T., Shirakawa, M., and Hiroaki, H.
J Biol Chem.
279
巻
(
48
)
頁：
50060-50068.
2004年11月
-
Crystallographic characterization of the N-terminal domain of PEX1.
査読有り
Shiozawa, K., Maita, N., Tomii, K., Seto, A., Goda, N., Akiyama, Y., Shimizu, T., Shirakawa, M., and Hiroaki, H.
Acta Crystallogr D Biol Crystallogr.
60
巻
(
11
)
頁：
2098-2099.
2004年11月
-
Two-Metal Ion, Ni(II) and Cu(II), Binding alpha-Helical Coiled Coil Peptide.
査読有り
Tanaka, T., Mizuno, T., Fukui, S., Hiroaki, H., Oku, J. I., Kanaori, K., Tajima, K., and Shirakawa, M.
J Am Chem Soc.
126
巻
(
43
)
頁：
14023-14028.
2004年10月
-
Structural basis for distinct roles of Lys63- and Lys48-linked polyubiquitin chains.
査読有り
Tenno, T., Fujiwara, K., Tochio, H., Iwai, K., Morita, E. H., Hayashi, H., Murata, S., Hiroaki, H., Sato, M., Tanaka, K., and Shirakawa, M.
Genes Cells.
9
巻
(
10
)
頁：
865-875.
2004年10月
-
High-throughput construction method for expression vector of peptides for NMR study suited for isotopic labeling.
査読有り
Tenno, T., Goda, N., Tateishi, Y., Tochio, H., Mishima, M., Hayashi, H., Shirakawa, M., and Hiroaki, H.
Protein Eng Des Sel.
17
巻
(
4
)
頁：
305-314.
2004年4月
-
The PRESAT-vector: asymmetric T-vector for high-throughput screening of soluble protein domains for structural proteomics.
査読有り
Goda, N., Tenno, T., Takasu, H., Hiroaki, H., and Shirakawa, M.
Protein Sci.
13
巻
(
3
)
頁：
652-658.
2004年3月
-
Structure of the ubiquitin-interacting motif of S5a bound to the ubiquitin-like domain of HR23B.
査読有り
Fujiwara, K., Tenno, T., Sugasawa, K., Jee, J. G., Ohki, I., Kojima, C., Tochio, H., Hiroaki, H., Hanaoka, F., and Shirakawa, M.
J Biol Chem.
279
巻
(
6
)
頁：
4760-4767.
2004年2月
-
Thermodynamic study of hybridization properties of heterochiral nucleic acids.
査読有り
Urata, H., Shimizu, H., Hiroaki, H., Kohda, D., and Akagi, M.
Biochem Biophys Res Commun.
309
巻
(
1
)
頁：
79-83.
2003年9月
-
1H, 13C and 15N backbone resonance assignments of the N-terminal domain of Drosophila GCM protein.
査読有り
Shimizu, M., Hiroaki, H., Kohda, D., Morita, E. H., Hotta, S., and Morikawa, K.
J Biomol NMR.
26
巻
(
3
)
頁：
277-278.
2003年7月
-
Phosphorylation of p47phox directs phox homology domain from SH3 domain toward phosphoinositides, leading to phagocyte NADPH oxidase activation.
査読有り
Ago, T., Kuribayashi, F., Hiroaki, H., Takeya, R., Ito, T., Kohda, D., and Sumimoto, H.
Proc Natl Acad Sci U S A.
100
巻
(
8
)
頁：
4474-4479.
2003年4月
-
NMR and ICP spectroscopic analysis of the DNA-binding domain of the Drosophila GCM protein reveals a novel Zn2+ -binding motif.
査読有り
Shimizu, M., Hiroaki, H., Kohda, D., Hosoya, T., Akiyama-Oda, Y., Hotta, Y., Morita, E. H., and Morikawa, K.
Protein Eng.
16
巻
(
4
)
頁：
247-254.
2003年4月
-
[Membrane associating protein domains: a new paradigm of phosphoinositide binding domains].
Hiroaki, H.
Tanpakushitsu Kakusan Koso.
46
巻
(
13
)
頁：
1936-1942.
2001年10月
-
The PX domain as a novel phosphoinositide- binding module.
査読有り
Ago, T., Takeya, R., Hiroaki, H., Kuribayashi, F., Ito, T., Kohda, D., and Sumimoto, H.
Biochem Biophys Res Commun.
287
巻
(
3
)
頁：
733-738.
2001年9月
-
Solution structure of the PX domain, a target of the SH3 domain.
査読有り
Hiroaki, H., Ago, T., Ito, T., Sumimoto, H., and Kohda, D.
Nat Struct Biol.
8
巻
(
6
)
頁：
526-530.
2001年6月
-
Characterization of DNA, RNA and DNA/RNA duplexes containing an L-nucleotide.
Urata, H., Shimizu, H., Hiroaki, H., Kohda, D., and Akagi, M.
Nucleic Acids Res Suppl.
1
巻
頁：
243-244.
2001年
-
Design of a Heterotrimeric alpha -Helical Bundle by Hydrophobic Core Engineering.
査読有り
Kashiwada, A., Hiroaki, H., Kohda, D., Nango, M., and Tanaka, T.
J Am Chem Soc.
122
巻
(
2
)
頁：
212 -215.
2000年12月
-
Soft metal ions, Cd(II) and Hg(II), induce triple-stranded alpha-helical assembly and folding of a de novo designed peptide in their trigonal geometries.
査読有り
Li, X., Suzuki, K., Kanaori, K., Tajima, K., Kashiwada, A., Hiroaki, H., Kohda, D., and Tanaka, T.
Protein Sci.
9
巻
(
7
)
頁：
1327-1333.
2000年7月
-
A'-form RNA double helix in the single crystal structure of r(UGAGCUUCGGCUC).
査読有り
Tanaka, Y., Fujii, S., Hiroaki, H., Sakata, T., Tanaka, T., Uesugi, S., Tomita, K., and Kyogoku, Y.
Nucleic Acids Res.
27
巻
(
4
)
頁：
949-955.
1999年2月
-
Influence of various nucleotides on the in situ crystallization of Ca2+-ATPase.
査読有り
Hiroaki, Y., Mitsuoka, K., Cheng, Y., Hiroaki, H., and Fujiyoshi, Y.
Biochim Biophys Acta.
1415
巻
(
2
)
頁：
361-368.
1999年1月
-
Metal Ion Induced Self-Assembly of a Designed Peptide into a Triple-Stranded a Helical Bundle: A Novel Metal Binding Site in the Hydrophobic Core.
査読有り
Suzuki, K., Hiroaki, H., Kohda, D., Nakamura, H., and Tanaka, T.
J Am Chem Soc.
120
巻
(
50
)
頁：
13008 -13015.
1998年12月
-
An isoleucine zipper peptide forms a native-like triple stranded coiled coil in solution.
査読有り
Suzuki, K., Hiroaki, H., Kohda, D., and Tanaka, T.
Protein Eng.
11
巻
(
11
)
頁：
1051-1055.
1998年11月
-
Determination of the solution structure of the SH3 domain of human p56 Lck tyrosine kinase.
査読有り
Hiroaki, H., Klaus, W., and Senn, H.
J Biomol NMR.
8
巻
(
2
)
頁：
105-122.
1996年9月
-
Cloning of boar SPMI gene which is expressed specifically in seminal vesicle and codes for a sperm motility inhibitor protein.
査読有り
Iwamoto, T., Hiroaki, H., Furuichi, Y., Wada, K., Satoh, M., Satoh, M., Osada, T., and Gagnon, C.
FEBS Lett.
368
巻
(
3
)
頁：
420-424.
1995年7月
-
Spectroscopic Characterization of the Decadeoxynucleotide Duplex Modified with Dichloroethylene-diaminoplatinum(II).
査読有り
Urata, H., Urata, M., Akagi, M., Hiroaki, H., and Uesugi, S.
Nucleosides & Nucleotides.
13
巻
頁：
1259-1269.
1994年7月
-
Properties Of A Hammerhead-Type Rna Enzyme-System That Consists Of 3 Rna Oligomer Strands.
査読有り
Odai, O., Hiroaki, H., Tanaka, T., and Uesugi, S.
Nucleosides & Nucleotides.
13
巻
頁：
1569-1579.
1994年7月
-
Properties of hammerhead-type RNA enzyme derivatives which contain a G-to-I replacement in the loop region.
Uesugi, S., Kodama, H., Hiroaki, H., and Odai, O.
Nucleic Acids Symp Ser.
27
巻
頁：
13-14.
1992年
-
Conformational features of the four successive non-Watson-Crick base pairs in RNA duplex.
Fujii, S., Tanaka, Y., Uesugi, S., Tanaka, T., Sakata, T., and Hiroaki, H.
Nucleic Acids Symp Ser.
27
巻
頁：
63-64.
1992年
-
Interaction of bleomycin with deoxyribonucleic acid oligomer: proton nuclear magnetic resonance titration study using novel bleomycin complexes with Ni2+ and VO3+.
査読有り
Hiroaki, H., Nakayama, T., Ikehara, M., and Uesugi, S.
Chem Pharm Bull (Tokyo).
39
巻
(
11
)
頁：
2780-2786.
1991年11月
-
Conformation of 2'GMP bound to a mutant ribonuclease T1 (Y45W) determined by X-ray diffraction and NMR methods.
査読有り
Itoh, T., Tomita, K., Hakoshima, T., Hiroaki, H., Uesugi, S., Nishikawa, S., Amisaki, T., Morioka, H., Ohtsuka, E., and Ikehara, M.
J Biochem (Tokyo).
110
巻
(
5
)
頁：
677-680.
1991年11月
-
The molecular recognition mechanism of DNA by bleomycin.
Hiroaki, H., Uesugi, S., and Fujii, S.
Nucleic Acids Symp Ser.
25
巻
頁：
147-148.
1991年
-
Molecular structure of extraordinarily stable RNA: model building and X-ray analysis.
Fujii, S., Tanaka, Y., Tomita, K., Sakata, T., Hiroaki, H., Tanaka, T., and Uesugi, S.
Nucleic Acids Symp Ser.
25
巻
頁：
181-182.
1991年
-
Synthesis and NMR study of ribo-oligonucleotides forming a hammerhead-type RNA enzyme system.
査読有り
Odai, O., Kodama, H., Hiroaki, H., Sakata, T., Tanaka, T., and Uesugi, S.
Nucleic Acids Res.
18
巻
(
20
)
頁：
5955-5960.
1990年10月
-
Studies on the structure and stabilizing factor of the CUUCGG hairpin RNA using chemically synthesized oligonucleotides.
査読有り
Sakata, T., Hiroaki, H., Oda, Y., Tanaka, T., Ikehara, M., and Uesugi, S.
Nucleic Acids Res.
18
巻
(
13
)
頁：
3831-3839.
1990年7月
-
The role of a conserved guanosine residue in the hammerhead-type RNA enzyme.
査読有り
Odai, O., Hiroaki, H., Sakata, T., Tanaka, T., and Uesugi, S.
FEBS Lett.
267
巻
(
1
)
頁：
150-152.
1990年7月
-
Conformation and properties of hammerhead-type RNA enzymes.
Uesugi, S., Odai, O., Hiroaki, H., Kodama, H., Sakata, T., and Tanaka, T.
Nucleic Acids Symp Ser.
22
巻
頁：
41-42.
1990年
-
Base recognition mechanism of bleomycin: solution structure of d(GGGGAGCTCCCC)2 based on 1H-NMR and restrained molecular dynamics.
Hiroaki, H., Ebata, T., Uesugi, S., and Fujii, S.
Nucleic Acids Symp Ser.
22
巻
頁：
53-54.
1990年
-
Identification of DNA degradation products by antitumor antibiotic, esperamicin.
Ebata, T., Hiroaki, H., Uesugi, S., and Sugiura, Y.
Nucleic Acids Symp Ser.
22
巻
頁：
19-20.
1990年
-
A 2-deoxyribonolactone-containing nucleotide: isolation and characterization of the alkali-sensitive photoproduct of the trideoxyribonucleotide d(ApCpA).
査読有り
Urata, H., Yamamoto, K., Akagi, M., Hiroaki, H., and Uesugi, S.
Biochemistry.
28
巻
(
25
)
頁：
9566-9569.
1989年12月
-
Novel 1H-31P heteronuclear coherence transfer spectroscopy based on spin locking MLEV-17 and its application to signal assignment for a short DNA fragment.
査読有り
Hiroaki, H., and Uesugi, S.
FEBS Lett.
244
巻
(
1
)
頁：
43-46.
1989年2月
-
Synthesis and properties of RNA with catalytic activity.
Odai, O., Sakata, T., Orita, M., Hiroaki, H., Uesugi, S., and Tanaka, T.
Nucleic Acids Symp Ser.
21
巻
頁：
105-106.
1989年
-
NMR studies on DNA hairpin structures with a five nucleotide loop.
Uesugi, S., Oda, Y., Hiroaki, H., and Ikehara, M.
Nucleic Acids Symp Ser.
21
巻
頁：
65-66.
1989年
