Faculty Profiles - ITO Akira

Papers - ITO Akira

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Medical Application of Functionalized Magnetic Nanoparticles Invited Reviewed

Akira Ito, Masashige Shinkai, Hiroyuki Honda, Takeshi Kobayashi

Journal of Bioscience and Bioengineering Vol. 100 ( 1 ) page： 1-11 2005

Cancer immunotherapy based on intracellular hyperthermia using magnetite nanoparticles: a novel concept of heat-controlled necrosis with heat shock protein expression Invited Reviewed

Akira Ito, Hiroyuki Honda, Takeshi Kobayashi

Cancer Immunology Immunotherapy Vol. 55 ( 3 ) page： 320-328 2005

In vitro drug testing based on contractile activity of C2C12 cells in an epigenetic drug model Reviewed

Kazushi Ikeda, Akira Ito, Ryusuke Imada, Masanori Sato, Yoshinori Kawabe, Masamichi Kamihira

SCIENTIFIC REPORTS Vol. 7 page： 44570 2017.3

Language：English Publishing type：Research paper (scientific journal) Publisher：NATURE PUBLISHING GROUP

Skeletal muscle tissue engineering holds great promise for pharmacological studies. Herein, we demonstrated an in vitro drug testing system using tissue-engineered skeletal muscle constructs. In response to epigenetic drugs, myotube differentiation of C2C12 myoblast cells was promoted in two-dimensional cell cultures, but the levels of contractile force generation of tissue-engineered skeletal muscle constructs prepared by three-dimensional cell cultures were not correlated with the levels of myotube differentiation in two-dimensional cell cultures. In contrast, sarcomere formation and contractile activity in two-dimensional cell cultures were highly correlated with contractile force generation of tissue-engineered skeletal muscle constructs. Among the epigenetic drugs tested, trichostatin A significantly improved contractile force generation of tissue-engineered skeletal muscle constructs. Follistatin expression was also enhanced by trichostatin A treatment, suggesting the importance of follistatin in sarcomere formation of muscular tissues. These observations indicate that contractility data are indispensable for in vitro drug screening.

DOI： 10.1038/srep44570

Web of Science

PubMed

Genetically engineered angiogenic cell sheets using magnetic force-based gene delivery and tissue fabrication techniques. Reviewed

Hirokazu Akiyama, Akira Ito, Yoshinori Kawabe, Masamichi Kamihira

Biomaterials Vol. 31 ( 6 ) page： 1251-1259 2010

Tissue engineering using magnetite nanoparticles and magnetic force: Heterotypic layers of co-cultured hepatocytes and endothelial cells. Reviewed

Akira Ito, Yohei Takizawa, Hiroyuki Honda, Kenichiro Hata, Hideaki Kagami, Minoru Ueda, Takeshi Kobayashi

Tissue Engineering Vol. 10 ( 5-6 ) page： 833-840 2004

Magnetically triggered transgene expression in mammalian cells by localized cellular heating of magnetic nanoparticles. Reviewed

Akira Ito, Ryoji Teranishi, Kazuki Kamei, Masaki Yamaguchi, Akihiko Ono, Shinya Masumoto, Yuto Sonoda, Masanobu Horie, Yoshinori Kawabe, Masamichi Kamihira

Journal of bioscience and bioengineering Vol. 128 ( 3 ) page： 355 - 364 2019.9

Language：English Publishing type：Research paper (scientific journal)

To develop a remote control system of transgene expression through localized cellular heating of magnetic nanoparticles, a heat-inducible transgene expression system was introduced into mammalian cells. Cells were labeled with magnetic nanoparticles and exposed to an alternating magnetic field. The magnetically labeled cells expressed the transgene in a monolayer and multilayered cell sheets in which cells were heated around the magnetic nanoparticles without an apparent temperature increase in the culture medium. Magnetic cells were also generated by genetically engineering with a ferritin gene, and transgene expression could be induced by exposure to an alternating magnetic field. This approach may be applicable to the development of novel gene therapies in cell-based medicine.

DOI： 10.1016/j.jbiosc.2019.03.008

PubMed

Neural differentiation of mouse induced pluripotent stem cells using cadherin gene-engineered PA6 feeder cells. Reviewed

Paerwen Paerhati, Akira Ito, Kantaro Yoshioka, Kaori Iwamoto, Sho Fujiwara, Masanobu Horie, Yoshinori Kawabe, Masamichi Kamihira

Journal of bioscience and bioengineering Vol. 127 ( 5 ) page： 633 - 640 2019.5

Language：English Publishing type：Research paper (scientific journal)

Investigating neural differentiation of pluripotent stem cells, including induced pluripotent stem (iPS) cells, is of importance for studying early neural development and providing a potential source of cells for nerve regeneration. Stromal cell-derived inducing activity (SDIA) using PA6 stromal cells promotes neural differentiation of iPS cells. Thus, we hypothesized that cadherin gene-engineered PA6 feeder cells will enhance the performance of SDIA by facilitating cell-cell interactions. Consequently, we created cadherin gene-engineered PA6 cells. Efficiency of neural differentiation from mouse iPS cells on PA6 feeder cells overexpressing E-cadherin gene (46%) or N-cadherin gene (27%) was significantly higher compared with parental PA6 feeder cells (19%). In addition, efficiency of motor neuron differentiation from mouse iPS cells on cadherin-gene engineered feeder cells (E-cadherin, 7.4%; N-cadherin, 11%) was significantly higher compared with parental PA6 feeder cells (4.1%). Altogether, these results indicate that cadherin gene-engineered feeder cells are a potent tool for promoting neural differentiation of pluripotent stem cells.

DOI： 10.1016/j.jbiosc.2018.10.009

PubMed

Fabricating Muscle-Neuron Constructs with Improved Contractile Force Generation. Reviewed

Arifuzzaman M, Ito A, Ikeda K, Kawabe Y, Kamihira M

Tissue engineering. Part A Vol. 25 ( 7-8 ) page： 563-574 2019.4

Induction of functional tissue-engineered skeletal muscle constructs by defined electrical stimulation. Reviewed

Akira Ito, Yasunori Yamamoto, Masanori Sato, Kazushi Ikeda, Masahiro Yamamoto, Hideaki Fujita, Eiji Nagamori, Yoshinori Kawabe, Masamichi Kamihira

Scientific Reports Vol. 4 page： 4781 2014

Improved recombinant antibody production by CHO cells using a production enhancer DNA element with repeated transgene integration at a predetermined chromosomal site Reviewed

Yoshinori Kawabe, Takanori Inao, Shodai Komatsu, Guan Huang, Akira Ito, Takeshi Omasa, Masamichi Kamihira

JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 123 ( 3 ) page： 390 - 397 2017.3

Language：English Publishing type：Research paper (scientific journal) Publisher：SOC BIOSCIENCE BIOENGINEERING JAPAN

Chinese hamster ovary (CHO) cells are one of the most useful host cell lines for the production of biopharmaceutical proteins. Although a series of production processes have been refined to improve protein productivity and cost performance, establishing producer cells is still time-consuming and labor-intensive. Recombinase-mediated site-specific gene integration into a predetermined chromosomal locus may enable predictable protein expression, reducing the laborious process of cell screening. We previously developed an accumulative site-specific gene integration system (AGIS) using Cre recombinase and mutated loxP sites for transgene integration and amplification in the CHO cell genome. Epigenetic modifier elements such as insulators are effective DNA cis-regulatory elements for stabilizing transgene expression. Here, we attempted to enhance transgene expression in recombinant CHO cells generated by AGIS using a production enhancer DNA element (PE) derived from the CHO genome. The PE was introduced into an expression unit for a recombinant scFv-Fc antibody. The effect on scFv-Fc productivity of PE position and orientation within the transgene was evaluated, while keeping the background chromosomal structure constant. For the optimal PE arrangement, scFv-Fc productivity was enhanced 2.6-fold compared with an expression unit without a PE. The enhancing effect of the PE on transgene expression was also observed when two or three PE-flanked expression units were inserted as tandem repeats. These results indicate that AGIS using the PE-flanked expression unit is a promising approach for establishing producer cell lines for biopharmaceutical protein production. (C) 2016, The Society for Biotechnology, Japan. All rights reserved.

DOI： 10.1016/j.jbiosc.2016.10.011

Web of Science

PubMed

Novel therapy for pancreatic fistula using adipose-derived stem cell sheets treated with mannose. Reviewed

Hirokazu Kaneko, Toshio Kokuryo, Yukihiro Yokoyama, Junpei Yamaguchi, Tokunori Yamamoto, Rei Shibata, Momokazu Gotoh, Toyoaki Murohara, Akira Ito, Masato Nagino

Surgery Vol. 161 ( 6 ) page： 1561–1569 2017

Targeted transgene insertion into the CHO cell genome using Cre recombinase-incorporating integrase-defective retroviral vectors. Reviewed

Yoshinori Kawabe, Takuya Shimomura, Shuohao Huang, Suguru Imanishi, Akira Ito, Masamichi Kamihira

Biotechnology and Bioengineering Vol. 113 ( 7 ) page： 1600-1610 2016

Improved contractile force generation of tissue-engineered skeletal muscle constructs by IGF-I and Bcl-2 gene transfer with electrical pulse stimulation. Reviewed

Kazushi Ikeda, Akira Ito, Masanori Sato, Yoshinori Kawabe, Masamichi Kamihira

Regenerative Therapy Vol. 3 page： 38-44 2016

A new in vitro co-culture model using magnetic force-based nanotechnology. Reviewed

Hiroki Takanari, Keiko Miwa, XianMing Fu, Jun-ichi Nakai, Akira Ito, Kousuke Ino, Hiroyuki Honda, Jun Kunishida, Satoru Konishi, Tobias Opthof, Marcel AG van der Heyden, Itsuo Kodama, Jong-Kook Lee

Journal of Cellular Physiology Vol. 231 ( 10 ) page： 2249-2256 2016

DNA damage-responsive transgene expression mediated by the p53 promoter with transcriptional amplification. Reviewed

Akihiko Ono, Akira Ito, Taiga Suzuki, Masaki Yamaguchi, Yoshinori Kawabe, Masamichi Kamihira

Journal of Bioscience and Bioengineering Vol. 120 ( 4 ) page： 463-466 2015

Effects of type IV collagen on myogenic characteristics of IGF-I gene-engineered myoblast cells. Reviewed

Akira Ito, Masahiro Yamamoto, Kazushi Ikeda, Masanori Sato, Yoshinori Kawabe, Masamichi Kamihira

Journal of Bioscience and Bioengineering. Vol. 119 ( 5 ) page： 596-603 2015

Magnetic-labeled feeder system for mouse pluripotent stem cells. Reviewed

Masanobu Horie, Akira Ito, Takeshi Maki, Yoshinori Kawabe, Masamichi Kamihira

Journal of Bioscience and Bioengineering. Vol. 119 ( 5 ) page： 614-616 2015

Improved transgene integration into the CHO cell genome using the Cre-loxP system. Reviewed

Takanori Inao, Yoshinori Kawabe, Takuro Yamashiro, Yujiro Kameyama, Xue Wang, Akira Ito, Masamichi Kamihira

Journal of Bioscience and Bioengineering. Vol. 120 ( 1 ) page： 99-106 2015

Fabrication of 3D tissue-like structure using magnetite nanoparticles and magnetic force.

Akira Ito, Kosuke Ino, Kazunori Shimizu, Hiroyuki Honda, Masamichi Kamihira

2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science 2006

磁性ナノ粒子を用いた組織工学手法の開発 Invited

井藤　彰、本多裕之

再生医療－日本再生医療学会雑誌 Vol. 5 ( 1 ) page： 84-90 2006

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