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Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

BACKGROUND: Neuroblastoma is one of the most common childhood solid tumors. Because tumor suppressor genes are often hypermethylated in cancers, DNA methylation has emerged as a target for cancer therapeutics. Nanaomycin A, an inhibitor of DNA methyltransferase 3B, which mediates de novo DNA methylation, reportedly induces death in several types of human cancer cells. OBJECTIVE: To study the antitumor activity of nanaomycin A against neuroblastoma cell lines and its mechanism. METHODS: The anti-tumor effect of nanaomycin A on neuroblastoma cell lines was evaluated based on cell viability, DNA methylation levels, apoptosis-related protein expression, and neuronal-associated mRNA expression. RESULTS: Nanaomycin A decreased genomic DNA methylation levels and induced apoptosis in human neuroblastoma cells. Nanaomycin A also upregulated the expression of mRNAs for several genes related to neuronal maturation. CONCLUSIONS: Nanaomycin A is an effective therapeutic candidate for treating neuroblastoma. Our findings also suggest that the inhibition of DNA methylation is a promising anti-tumor therapy strategy for neuroblastoma.

DOI： 10.2174/1568009623666230522113645

PubMed

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Language：English   Publishing type：Research paper (scientific journal)  

Osteoclasts play a crucial role in osteolytic bone diseases, such as osteoporosis, rheumatoid arthritis, periodontitis, Paget's disease of bone and bone metastatic tumors. Therefore, controlling osteoclast differentiation and function has been considered a promising therapeutic strategy. Here, we show that necrostatin (Nec)-7, an inhibitor of programmed necrosis, strongly suppressed receptor activator of nuclear factor (NF)-κB ligand (RANKL)-induced osteoclastogenesis and bone resorption, without compromising macrophage colony-stimulating factor (M-CSF)-supported survival and growth of osteoclast precursor cells. Accordingly, Nec-7 significantly decreased the levels of RANKL-induced osteoclastogenic marker genes, such as cathepsin K. Mechanistically, Nec-7 neither affected MAPK nor NF-κB activation; however, it strongly inhibited the RANKL receptor (RANK) to nuclear factor of activated T cells c1 (NFATc1) signaling. Lentiviral expression of RANK in bone marrow-derived macrophages significantly restored osteoclastogenesis and NFATc1 amplification in Nec-7-treated cells. In this study, we revealed that Nec-7-sensitive pathways are crucially involved in osteoclast formation and function. Investigation of the molecular mechanism(s) through which Nec-7 inhibits RANK-NFATc1 signaling axis may lead to the development of new therapeutic strategies for bone disease.

DOI： 10.1016/j.bbrc.2018.05.153

PubMed

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Authorship：Lead author   Language：English   Publishing type：Research paper, summary (national, other academic conference)   Publisher：Japanese Pharmacological Society  

DOI： 10.1254/jpssuppl.97.0_2-b-p-091

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Publishing type：Research paper, summary (national, other academic conference)  

J-GLOBAL

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Publishing type：Research paper, summary (national, other academic conference)  

J-GLOBAL

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Authorship：Lead author   Language：Japanese   Publishing type：Research paper, summary (national, other academic conference)   Publisher：日本薬理学会  

DOI： 10.1254/fpj.s20061

J-GLOBAL

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Authorship：Lead author   Publishing type：Research paper, summary (national, other academic conference)  

J-GLOBAL

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Event date： 2025.3

Language：Japanese   Presentation type：Poster presentation  

Country：Japan  

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Event date： 2024.9

Language：English   Presentation type：Poster presentation  

Venue：Graduate School of Pharmaceutical Sciences, Nagoya City University   Country：Japan  

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Event date： 2023.12

Language：Japanese   Presentation type：Poster presentation  

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Event date： 2020.6

Language：Japanese   Presentation type：Oral presentation (general)  

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Event date： 2019.9

Language：Japanese   Presentation type：Oral presentation (general)  

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◆主 催：公益社団法人日本薬学会薬学教育委員会
◆参加者：大学院生および博士取得者（取得5年未満）

博士取得（予定も含む）者の多くは，高度な学術的知識や問題解決能力を有している．しかしながら，新たなキャリアへの移行や進路選択においては，種々の課題に直面することもある．本ワークショップでは，参加者が新たなキャリアへの挑戦において自信を持って立ち向かい，将来の夢の実現につなげる一助になることを目的とした．すなわち，本ワークショップのテーマに積極的に取り組み，学問領域や大学・職場の枠組みを越えて，学術研究やキャリアデザインについて意見交換することで，新たなキャリアの扉が開かれることを期待したものである．

DOI： 10.14894/faruawpsj.60.4_357

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オンサイトの技術講習会では「In situ hybridizationの実際」(菱川善隆先生) を受講し、ISH の基本的な技術についてご指導いただきました。
(概要) この講習会では、初学者にも分かりやすい講義と技術講習会を通して、組織細胞化学に関連した重要な基礎知識と応用技術の習得を目指します。

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Other Link： http://jshc.nacos.com/kosyu/

We explore techniques and methods to quantify the physical properties that determine the dynamics of biological phenomena. Our main focus is on cellular-level biology, but we are also concerned with the structure and organization of cells. The behavior of cells is influenced by events at a molecular level and upwards to the tissue and organism level. A new-generation model of Q-BioJP can take initiatives at medical sciences such as pharmacokinetics and cancer research. Q-BioJP will strive to understand the various levels of biological systems and the relationships that exist between them.

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名古屋市立大学の泉和弥氏が来訪し、「破骨細胞分化ダイナミクスの数理モデリングと破骨細胞分化メカニズムの解析」のタイトルでラボセミナーをしていただきました。
泉氏は、朝霧教授の前任地である京都大学に国内留学してきてくれた共同研究者です。

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Certificate number: 1952

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Certificate number: 00988

添付ファイル： 統計検定 専門統計調査士_certificate.pdf

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Certificate number: 19-376

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Certificate of Instructor-Led Training Course on BD FACSAria™ Cell Sorter (on-site)

添付ファイル： FACS修了証.pdf

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Audience： High school students,　Guardians,　General

Type：University open house

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Audience： Infants,　Schoolchildren,　Junior students,　High school students,　Teachers,　Guardians,　General

Type：Seminar, workshop

As a representative lecturer, he demonstrated the experiment and held a Q&A session with the participants.

Title: "Let's make a superball !"
Summary: Children made a superball about 3-4 cm in diameter using latex and citric acid. Instructors let children stir and harden the latex themselves.

File： 祭典プログラム集2014.pdf

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Audience： Infants,　Schoolchildren,　Junior students,　High school students,　Teachers,　Guardians,　General

Type：Seminar, workshop

I demonstrated the experiment and held a Q&A session with the participants.

Title: "Let's make a superball !"
Summary: Using latex and citric acid, children made a superball about 3-4 cm in diameter. Instructors let the children stir and harden the latex themselves.

File： 祭典プログラム集2012.pdf

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