Updated on 2025/04/04

写真a

 
TSUKADA Kotaro
 
Organization
Research Institute of Environmental Medicine Division of Stress Adaptation and Protection Designated assistant professor
Title
Designated assistant professor
Contact information
メールアドレス
Homepage
https://www.facebook.com/kotaro.tsukada.98/
External link

Degree 1

  1. Doctor of Science （ 2023.3   Saitama University ） 

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Research Interests 1

  1. DNA repair

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Research Areas 4

  1. Life Science / Genetics

  2. Life Science / Cell biology

  3. Life Science / Structural biochemistry

  4. Life Science / Molecular biology

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Research History 2

  1. Nagoya University   Research Institute of Environmental Medicine   Assistant Professor

    2025.3

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  2. The University of Tokyo   Institute for Quantitative Biosciences   Project Researcher

    2023.4 - 2025.2

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Education 4

  1. Saitama University   Graduate School of Science and Engineering   Science and Engineering, Life Science Course, Doctoral Degree Program

    2020.4 - 2023.3

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    Country： Japan

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  2. Saitama University   Graduate School of Science and Engineering   Life Science, Regulatory Biology Program, Master's Degree Program

    2018.4 - 2020.3

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    Country： Japan

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  3. Toyo University   Faculty of Life Sciences   Department of Life Science

    2014.4 - 2018.3

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    Country： Japan

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  4. Yachiyo High School   Physical Education Course

    2011.4 - 2014.3

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    Country： Japan

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Awards 4

  1. Repayment Exemption for Students with Excellent Grades, Japan Student Services Organization Scholarship (2022)

    2022   Japan Student Services Organization  

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  2. Saitama University Excellent Academic Performance Award (2021)

    2021   Saitama University  

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  3. Saitama University Excellent Academic Performance Award (2019)

    2019   Saitama University  

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  4. Repayment Exemption for Students with Excellent Grades, Japan Student Services Organization Scholarship (2019)

    2019   Japan Student Services Organization  

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Papers 3

  1. A protocol for genetic analysis at different stages of the nuclear division cycle in Neurospora crassa Reviewed International journal Open Access

    Kotaro Tsukada, Shin Hatakeyama, Shuuitsu Tanaka

    Fungal Genetics Reports   Vol. 67 ( 1 ) page： 1   2023.8

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    The filamentous fungus Neurospora crassa is an organism that contains multiple nuclei in the asexual conidia and hyphae. Since the nuclei of dormant conidia are arrested at various points in the nuclear division cycle, it has been difficult to analyze drug sensitivity at the specific point of the cycle in N. crassa. In this study, we have stablished a useful method for analysis at different stages of the nuclear division cycle in N. crassa. This assay will be a reference for researchers to use the synchronized culture in other diverse analyses.

    Open Access

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  2. DNA interstrand crosslink repair by XPF-ERCC1 homologue confers ultraviolet resistance in Neurospora crassa Reviewed International journal Open Access

    Kotaro Tsukada, Shin Hatakeyama, Shuuitsu Tanaka

    Fungal genetics and biology   Vol. 164   page： 103752   2023.1

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    Ultraviolet (UV) light is a mutagen that causes DNA damage. Some UV-sensitive Neurospora crassa strains have been reported to exhibit a partial photoreactivation defect (PPD) phenotype, and the possible cause of this has been unknown for more than half a century. In this study, in the process of elucidating the possible causes of a PPD phenotype, we discovered that the XPF homologue MUS-38 is involved in repairing the UV-induced DNA interstrand crosslink (ICL) in N. crassa. Furthermore, the sensitivity of the Δmus-38 and Δmus-44 strains to ICL agents was significantly higher than that of other nucleotide excision repair (NER)-related gene knockout (KO) strains, indicating that the MUS-38/MUS-44 complex is involved in an NER-independent ICL repair mechanism. Based on reports concerning the mammalian homologues XPF and ERCC1 we obtained separation-of-function mutants defective only in NER in mus-38 and mus-44. Additionally, the photoreactivation ability of these mutants was significantly higher than that of the KO strains. These results indicate that the PPD phenotype is caused by a defect in the repair-ability of ICL induced by UV and that an NER-independent ICL repair by MUS-38 and MUS-44 confers resistance to UV in N. crassa.

    DOI： 10.1016/j.fgb.2022.103752

    PubMed

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  3. A partial photoreactivation defect phenotype is not due to unrepaired ultraviolet-induced pyrimidine dimers in ultraviolet-sensitive mutants of Neurospora crassa Reviewed International journal Open Access

    Kotaro Tsukada, Ryouhei Yoshihara, Shin Hatakeyama, Akihiko Ichiishi, Shuuitsu Tanaka

    Genes & genetic systems   Vol. 95 ( 6 ) page： 281 - 289   2020.12

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    Photoreactivation is a mechanism in which photolyase directly repairs either cyclobutane pyrimidine dimers (CPDs) or (6-4) photoproducts [(6-4) PPs] caused by ultraviolet (UV) light. In the filamentous fungus Neurospora crassa, some UV-sensitive mutants such as mus-44 have been reported to exhibit a partial photoreactivation defect (PPD) phenotype, but its mechanism has not been elucidated for a long time. In this study, the N. crassa CPD photolyase PHR was overexpressed in the Δmus-44 strain, but photoreactivation ability was not increased. Furthermore, Escherichia coli CPD photolyase or Arabidopsis thaliana (6-4) PP photolyase was also introduced into Δmus-44; however, the PPD phenotype was not complemented. These results suggested that the PPD phenotype in N. crassa is not caused by residual unrepaired pyrimidine dimers, which are the main type of DNA damage caused by UV irradiation. Finally, we revealed that Δmus-44, but not the Δmus-43 strain, which does not show the PPD phenotype, displayed higher sensitivity with increasing dose rate of UV. Moreover, Δmus-44 was also sensitive to an interstrand crosslinking agent. This indicates that the high dose of UV in our experimental condition induces DNA damage other than pyrimidine dimers, and that such damage is a likely cause of the PPD phenotype.

    DOI： 10.1266/ggs.20-00022

    Open Access

    PubMed

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Presentations 14

  1. Mechanism of immune signalling factor sequestration on chromatin by the adenovirus core protein VII International conference

    Kotaro Tsukada, Suguru Hatazawa, Melanie Dillon, Daphne C. Avgousti, Yoshimasa Takizawa, Hitoshi Kurumizaka

    The 12th 3R+3C International Symposium  2024.11 

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    Event date： 2024.11

    Language：English   Presentation type：Poster presentation  

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  2. Mechanism of immune signal factor suppression on chromatin by adenovirus histone-like protein

    Kotaro Tsukada, Suguru Hatazawa, Melanie Dillon, Daphne C. Avgousti, Yoshimasa Takizawa, Hitoshi Kurumizaka

    The 1st Meeting of Transformative Research Areas (A) Deciphering the epicode of chromatin, which controls cell fate decisions in organisms  2024.7 

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    Event date： 2024.7

    Presentation type：Poster presentation  

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  3. Effects of adenovirus protein VII on HMGB1-mediated chromatin structure

    Kotaro Tsukada, Suguru Hatazawa, Melanie Dillon, Yoshimasa Takizawa, Daphne C. Avgousti, Hitoshi Kurumizaka

    The 17th Annual Meeting of the Japanese Society for Epigenetics  2024.6 

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    Event date： 2024.6

    Presentation type：Poster presentation  

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  4. Structural change of HMGB1-bound chromatin by adenovirus protein VII

    Kotaro Tsukada, Suguru Hatazawa, Melanie Dillon, Yoshimasa Takizawa, Daphne C. Avgousti, Hitoshi Kurumizaka

    JST-EMBO Matchmaking Workshop  2024.3 

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    Event date： 2024.3

    Language：English   Presentation type：Poster presentation  

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  5. DNA repair pathway choice of the SNM1A nuclease

    Kotaro Tsukada, Shin Hatakeyama, ShuuitsuTanaka

    The 94th Annual Meeting of the Genetics Society of Japan  2022.9 

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    Event date： 2022.9

    Presentation type：Oral presentation (general)  

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  6. Development of an assay that synchronises the nuclear division cycle in Neurospora crassa

    Kotaro Tsukada, Ryouhei Yoshihara, Shin Hatakeyama, Shuuitsu Tanaka

    The 44th Annual Meeting of the Molecular Biology Society of Japan  2021.12 

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    Event date： 2021.12

    Presentation type：Poster presentation  

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  7. Analysis of the SLX4 homologue concerning to DNA interstrand crosslink repair in Neurospora crassa

    Kotaro Tsukada, Ryouhei Yoshihara, Shin Hatakeyama, Shuuitsu Tanaka

    The 93th Annual Meeting of the Genetics Society of Japan  2021.9 

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    Event date： 2021.9

    Presentation type：Oral presentation (general)  

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  8. Identification and functional analysis of SLX1-SLX4 nuclease in Neurospora crassa

    Kotaro Tsukada, Ryouhei Yoshihara, Shin Hatakeyama, Shuuitsu Tanaka

    The 2021 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry  2021.3 

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    Event date： 2021.3

    Presentation type：Oral presentation (general)  

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  9. Epistasis analysis of the FAN1 and SNM1A homologues in Neurospora crassa

    Kotaro Tsukada, Ryouhei Yoshihara, Shin Hatakeyama, Shuuitsu Tanaka

    The 43th Annual Meeting of the Molecular Biology Society of Japan  2020.12 

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    Event date： 2020.12

    Presentation type：Poster presentation  

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  10. Genetic analysis of nucleases involved in DNA interstrand crosslink repair

    Kotaro Tsukada, Ryouhei Yoshihara, Shin Hatakeyama, Shuuitsu Tanaka

    The 92th Annual Meeting of the Genetics Society of Japan  2020.9 

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    Event date： 2020.9

    Presentation type：Oral presentation (general)  

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  11. A functional analysis of the XPF-ERCC1 in Neurospora crassa

    Kotaro Tsukada, Ryouhei Yoshihara, Shin Hatakeyama, Shuuitsu Tanaka

    The 42th Annual Meeting of the Molecular Biology Society of Japan  2019.12 

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    Event date： 2019.12

    Presentation type：Poster presentation  

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  12. A partial photoreactivation defect phenotype in Neurospora crassa is caused by impairment of DNA interstrand crosslink repair

    Kotaro Tsukada, Ryouhei Yoshihara, Shin Hatakeyama, Shuuitsu Tanaka

    The 91th Annual Meeting of the Genetics Society of Japan  2019.9 

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    Event date： 2019.9

    Presentation type：Oral presentation (general)  

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  13. Functional analysis of the ERCC1 homologue in Neurospora crassa －The relationship between sensitvity to interstrand crosslinking agents and abnormality of photoreactivation－

    Kotaro Tsukada, Akihiko Ichiishi, Ryouhei Yoshihara, Shin Hatakeyama, Shuuitsu Tanaka

    The 2019 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry  2019.3 

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    Event date： 2019.3

    Presentation type：Oral presentation (general)  

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  14. Analysis of photoreactivation ability of NER-deficient strains in Neurospora crassa

    Kotaro Tsukada, Akihiko Ichiishi

    The 2018 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry  2018.3 

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    Event date： 2018.3

    Presentation type：Oral presentation (general)  

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Research Project for Joint Research, Competitive Funding, etc. 4

  1. Structural biology study of the nucleosome receptor for the development of immunosuppressants against systemic lupus erythematosus

    2024.4 - 2025.3

    Hoyu Science Foundation  Research Grant for the fiscal year 2024 

    Kotaro Tsukada

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\500000 （ Direct Cost: \500000 ）

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  2. Analysis of SNM1A for the development of inhibitors that promote the therapeutic effects of anticancer drugs -Genetic approach by using Neurospora crassa-

    2022.4 - 2023.3

    Japan Science Society  Sasakawa Scientific Research Grant 

    Kotaro Tsukada

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\580000 （ Direct Cost: \580000 ）

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  3. Functional analysis of the SNM1A nuclease involved in DNA interstrand crosslink repair -Genetic study of Neurospora crassa as a model organism-

    2022.4 - 2023.3

    Toyo University  INOUE ENRYO Memorial Grant 

    Kotaro Tsukada

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\560000 （ Direct Cost: \560000 ）

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  4. Analysis of interstrand crosslink repair and SLX4 homologue in Neurospora crassa

    2021.4 - 2022.3

    Toyo University  INOUE ENRYO Memorial Grant 

    Kotaro Tsukada

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\560000 （ Direct Cost: \560000 ）

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KAKENHI (Grants-in-Aid for Scientific Research) 1

  1. Structural basis of inflammatory induction mechanisms by extracellular nucleosomes

    Grant number：24K18462  2024.4 - 2026.3

    Japan Society for the Promotion of Science  KAKENHI  Grant-in-Aid for Early-Career Scientists

    Kotaro Tsukada

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\4680000 （ Direct Cost: \3600000 、 Indirect Cost：\1080000 ）

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