Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Protein Science  

AlphaFold protein structure database (AlphaFold DB) archives a vast number of predicted models. We conducted systematic data mining against AlphaFold DB and discovered an uncharacterized P-loop NTPase family. The structure of the protein family was surprisingly novel, showing an atypical topology for P-loop NTPases, noticeable twofold symmetry, and two pairs of independent putative active sites. Our findings show that structural data mining is a powerful approach to identifying undiscovered protein families.

DOI： 10.1002/pro.4934

Web of Science

Scopus

PubMed

Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： https://doi.org/10.1038/s41594-023-01147-9

DOI： 10.1038/s41594-023-01147-9

DOI： https://doi.org/10.3390/biology12020182

Language：English

We introduce our approach to analyzing the entire Protein Data Bank (PDB) by combining state-of-the-art bioinformatic tools. As an interesting case, we report sequence/conformation analysis of Walker-A motifs and β-α-β supersecondary structures with/without this motif. Statistical analysis revealed that Walker-A motifs strongly correlate with β-α-β units having one or two intervening β-strands, while in general β-α-β units tend to exhibit direct contacts between the β-strands without intervening β-strands.

DOI： 10.1007/978-1-0716-4213-9_6

Scopus

PubMed

Total pages：7   Responsible for pages：7   Language：Japanese Book type：Textbook, survey, introduction

DOI： 10.18958/7335-00001-0000603-00