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Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

BACKGROUND: Existing proteomic studies have primarily focused on gout flares or symptomatic hyperuricaemia, and few have comprehensively investigated circulating proteomic profiles in serum urate (SU) concentration as a continuous quantitative trait. This cross-sectional study investigated the association between SU concentration and serum proteomic profiles. METHODS: We enrolled 176 Japanese individuals aged ≥50 years and measured serum proteins using the Olink Explore 3072. To analyse the association between SU concentration and serum proteins, we applied linear regression adjusting for age, sex, renal function and insulin resistance with a false discovery rate threshold of 5%. Tissue-specific and gene set enrichment analyses were conducted based on the regression results. RESULTS: A total of 2886 proteins were analysed, among whom 63 showed significant associations with SU concentration; uromodulin demonstrated the strongest association (adjusted p=2.21×10-5). These 63 SU-associated proteins were significantly enriched in the liver, kidneys and duodenum by tissue-specific enrichment analysis. We further examined p.Q141K-a dysfunctional variant of adenosine triphosphate-binding cassette subfamily G member 2 (ABCG2). Among p.Q141K minor allele carriers (n=91), 11 SU-associated proteins were identified, whereas no significant SU-associated proteins were detected in wild-type homozygotes (n=85). Gene set enrichment analysis highlighted xenobiotic metabolism in wild-types and additional inflammation- and disease-related gene sets in minor allele carriers. CONCLUSIONS: To the best of our knowledge, this is the first study to report a proteomic signature of SU concentration. These findings suggest that SU-associated proteomic signatures vary according to ABCG2 genotypes, highlighting the genetic contribution to pathophysiological processes associated with SU concentration variation. TRIAL REGISTRATION NUMBER: NCT00262691.

DOI： 10.1136/rmdopen-2025-006414

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Language：English   Publisher：Clinica Chimica Acta  

The authors regret that the name of co-author Kazuhiko Kotani was incorrectly published as Kazuhiro Kotani. The authors would like to apologise for any inconvenience caused.

DOI： 10.1016/j.cca.2025.120652

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Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

The 36-item Short-Form Health Survey (SF-36) is widely used to assess health-related quality of life. However, only few studies have examined longitudinal SF-36 trajectories in general populations, and even fewer have explored their relationships with lifestyle factors, including sleep quality. We conducted a longitudinal analysis of SF-36 subscale trajectories among 910 Japanese individuals aged ≥ 60 years from 2007 to 2018 (4,799 records). Using latent class mixed models, we identified distinct trajectory patterns for each subscale. Notably, the role-physical (RP) and role-emotional (RE) subscales exhibited multiple patterns, including rapid decline and non-decline trajectories. We further examined predictors that differentiated between these two trajectory patterns-rapid decline and non-decline-with similar baseline scores. Consequently, poor performance on the open-eye one-leg standing test and higher depression scores on the Centre for Epidemiologic Studies Depression Scale predicted RP and RE declines, respectively. Importantly, poor sleep quality, as measured by the Pittsburgh Sleep Quality Index-particularly the total score and component 7 (daytime dysfunction)-was associated with subsequent RP and RE declines. In contrast, sleep timing variables (bedtime, sleep onset, and wake-up time) were not predictive. These findings suggest that preserving good sleep quality may help prevent future limitations in daily physical and emotional roles.

DOI： 10.1038/s41598-025-30307-8

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Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

INTRODUCTION: More than 800 million patients currently have chronic kidney disease (CKD), placing a tremendous burden on both the healthcare and welfare economies. Habitual physical activity in patients with CKD may extend healthy life expectancy without functional disabilities; however, the relationship remains unclear. This study aimed to investigate the impact of habitual physical activity on the association between CKD and long-term nursing care certification. METHODS: This observational cohort study enrolled participants aged >64 years without long-term care certification based on functional disabilities. Habitual physical activity data were obtained using questionnaires, and 8428 participants were classified into the Kidney Disease Improving Global Outcomes risk categories at baseline: no CKD (n=5717), moderately increased risk (n=1980), high risk (n=542) and very high risk (n=189) groups. The primary outcome was long-term care certification, and HRs were estimated using the no CKD group with habitual physical activity as reference. RESULTS: Among the participants with habitual physical activity, very high risk CKD significantly increased the risk of functional disabilities requiring long-term care (HR 1.64, 95% CI 1.04 to 2.58). Meanwhile, non-habitual physical activity significantly increased the risk of functional disabilities with CKD progression, with the HRs for the no CKD, moderately increased risk, high risk and very high risk groups being 1.35 (95% CI 1.16 to 1.57), 1.30 (95% CI 1.07 to 1.58), 1.51 (95% CI 1.16 to 1.96) and 2.30 (95% CI 1.62 to 3.26), respectively. CONCLUSIONS: CKD progression is related to the risk of developing functional disabilities requiring long-term care. Habitual physical activity can help extend healthy life expectancy and reduce CKD-related welfare burden.

DOI： 10.1136/bmjph-2025-003138

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Many biobanks store biological samples and use them for various analyses, including proteomics. Therefore, it is necessary to consider the denaturation of target proteins during long-term storage. We analyzed 16-year-old cryopreserved serum samples using the SomaScan platform, a novel proteomic assay, to determine whether adiponectin and resistin concentrations were consistent with those measured in our previous studies using a different platform. The results suggested that long-term cryopreserved serum samples could be used for future studies of at least adiponectin and resistin, which are closely related to the pathophysiology of diabetes, cardiovascular disease, and other metabolic diseases. Therefore, 7,289 SomaScan-assayed circulating proteins were compared between 20 men and 20 women aged ≥ 50 to determine sex differences. In total, 20 serum proteins showed significant sex differences. Of these, proteins that showed a more than two-fold difference in concentration between sexes contained heterodimeric forms of gonadotropic proteins such as CGA|FSHB, CGA|CGB3|CGB7, and CGA|LHB, which are the biologically active forms of these hormones. The present study is the first to report the possibility of using long-term cryopreserved serum samples for the SomaScan assay, and the results show that the SomaScan assay may be useful for analyzing sex differences focusing on gonadotropic hormones.

DOI： 10.1038/s41598-025-14464-4

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Event date： 2025.9

Language：English   Presentation type：Oral presentation (invited, special)  

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Event date： 2024.2 - 2024.3

Language：Japanese   Presentation type：Oral presentation (general)  

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Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Language：English   Presentation type：Poster presentation  

Country：Austria  

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透析患者の尿酸値上昇と腸管ABCG2の機能低下の関連性

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