Organization
Graduate School of Bioagricultural Sciences Avian Bioscience Research Center Assistant Professor
Graduate School
Graduate School of Bioagricultural Sciences
Title
Assistant Professor
Contact information
External link
OKUZAKI Yuya
Degree 1
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Doctor (Engineering) （ 2017.12 Nagoya University ）
Research Interests 4
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transgenic chicken
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gene engineering
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genome editing
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primordial germ cells
Research Areas 2
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Life Science / Genome biology / genome editing, gene engineering, transgenic birds
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Life Science / Animal production science / genome editing, gene engineering, transgenic birds
Research History 2
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Nagoya University Graduate School of Bioagricultural Sciences Avian Bioscience Research Center Assistant Professor
2022.7
Country：Japan
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Nagoya University Graduate School of Bioagricultural Sciences Avian Bioscience Research Center Researcher
2020.4 - 2022.6
Country：Japan
Professional Memberships 4
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日本動物細胞工学会
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日本分子生物学会
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日本生物工学会
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日本ゲノム編集学会
Papers 13
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Nature communications Vol. 11 ( 1 ) page： 1334 2020.3
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CRISPR-Cas3 induces broad and unidirectional genome editing in human cells. Reviewed
Hiroyuki Morisaka, Kazuto Yoshimi, Yuya Okuzaki, Peter Gee, Yayoi Kunihiro, Ekasit Sonpho, Huaigeng Xu, Noriko Sasakawa, Yuki Naito, Shinichiro Nakada, Takashi Yamamoto, Shigetoshi Sano, Akitsu Hotta, Junji Takeda, Tomoji Mashimo
Nature communications Vol. 10 ( 1 ) page： 5302 2019.12
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PRDM14 and BLIMP1 control the development of chicken primordial germ cells. Reviewed
Yuya Okuzaki, Hidenori Kaneoka, Takayuki Suzuki, Yota Hagihara, Yuki Nakayama, Seitaro Murakami, Yusuke Murase, Atsushi Kuroiwa, Shinji Iijima, Ken-Ichi Nishijima
Developmental biology Vol. 455 ( 1 ) page： 32 - 41 2019.11
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Dual CRISPR-Cas3 system for inducing multi-exon skipping in DMD patient-derived iPSCs Reviewed
Stem Cell Reports Vol. 18 ( 9 ) page： 1753 - 1765 2023.9
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Regulatory mechanism of chicken lysozyme gene expression in oviducts examined using transgenic technology. Reviewed
Yusuke Kojima, Yuya Okuzaki, Ken-Ichi Nishijima, Shuichiro Moriwaki, Seiya Asai, Hidenori Kaneoka, Shinji Iijima
Journal of bioscience and bioengineering Vol. 131 ( 4 ) page： 453 - 459 2021.4
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Primordial germ cell-specific expression of eGFP in transgenic chickens. Invited Reviewed
Yota Hagihara, Yuya Okuzaki, Kazuma Matsubayashi, Hidenori Kaneoka, Takayuki Suzuki, Shinji Iijima, Ken-Ichi Nishijima
Genesis Vol. 58 ( 8 ) page： e23388 2020.8
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Identification of transgene integration site and anatomical properties of fluorescence intensity in a EGFP transgenic chicken line. Reviewed
Kaori Tsujino, Yuya Okuzaki, Nobuyuki Hibino, Kazuki Kawamura, Seiji Saito, Yumi Ozaki, Satoshi Ishishita, Atsushi Kuroiwa, Shinji Iijima, Yoichi Matsuda, Kenichi Nishijima, Takayuki Suzuki
Development, growth & differentiation Vol. 61 ( 7-8 ) page： 393 - 401 2019.9
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Molecular cloning of chicken TET family genes and role of chicken TET1 in erythropoiesis. Reviewed
Yuya Okuzaki, Hidenori Kaneoka, Ken-Ichi Nishijima, Seitaro Murakami, Yuki Ozawa, Shinji Iijima
Biochemical and biophysical research communications Vol. 490 ( 3 ) page： 753 - 759 2017.8
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Shunsuke Kidani, Yuya Okuzaki, Hidenori Kaneoka, Seiya Asai, Seitaro Murakami, Yusuke Murase, Shinji Iijima, Ken-Ichi Nishijima
Cytotechnology Vol. 69 ( 3 ) page： 477 - 484 2017.6
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Characterization of chicken interferon-inducible transmembrane protein-10. Reviewed
Yuya Okuzaki, Shunsuke Kidani, Hidenori Kaneoka, Shinji Iijima, Ken-Ichi Nishijima
Bioscience, biotechnology, and biochemistry Vol. 81 ( 5 ) page： 914 - 921 2017.5
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Analyses of chicken sialyltransferases related to O-glycosylation. Reviewed
Shunsuke Kidani, Hidenori Kaneoka, Yuya Okuzaki, Seiya Asai, Yusuke Kojima, Ken-Ichi Nishijima, Shinji Iijima
Journal of bioscience and bioengineering Vol. 122 ( 4 ) page： 379 - 384 2016.10
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Analyses of chicken sialyltransferases related to N-glycosylation. Invited Reviewed
Yusuke Kojima, Akifumi Mizutani, Yuya Okuzaki, Ken-Ichi Nishijima, Hidenori Kaneoka, Takako Sasamoto, Katsuhide Miyake, Shinji Iijima
Journal of bioscience and bioengineering Vol. 119 ( 6 ) page： 623 - 628 2015.6
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Recombinant proteins produced into yolk of genetically manipulated chickens are partly sialylated in N-glycan. Invited Reviewed
Kazuhiro Yoshida, Yuya Okuzaki, Ken-Ichi Nishijima, Kenji Kyogoku, Takashi Yamashita, Yoshinori Kawabe, Makoto Motono, Masamichi Kamihira, Shinji Iijima
Cytotechnology Vol. 65 ( 6 ) page： 985 - 992 2013.12
Presentations 2
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Development of the SpCas9 constitutively expressing transgenic chicken International conference
Yuya OKUZAKI
Avian Model Systems 11 2023.9.12
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SpCas9発現遺伝子組換えニワトリの作製
奥嵜雄也
日本ゲノム編集学会第8回大会 2023.6.7 日本ゲノム編集学会
KAKENHI (Grants-in-Aid for Scientific Research) 2
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ゲノム編集技術を用いたインフルエンザウイルス抵抗性ニワトリの作製に向けた研究
Grant number：22K15027 2022.4 - 2027.3
科学研究費助成事業 若手研究
奥嵜 雄也
Authorship：Principal investigator
Grant amount：\4550000 （ Direct Cost: \3500000 、 Indirect Cost：\1050000 ）
本研究では、ゲノム編集技術であるCRISPR-Cas9を用い、ニワトリ細胞において鳥インフルエンザウイルスの感染や増殖に必要な宿主遺伝子のスクリーニングを行う。このスクリーニングにより見出されたインフルエンザウイルスに抵抗性を示すと考えられる候補遺伝子を、実際にニワトリ個体でゲノム編集することで、インフルエンザ抵抗性ゲノム編集ニワトリ系統の作製を目指す。
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Development of CRISPR-Cas3 delivery system using virus-like particles
Grant number：20K15776 2020.4 - 2022.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists
OKUZAKI Yuya
CRISPR-Cas3 system is the novel genome editing technology which can efficiently introduce large-scale deletion on the genome. Although CRISPR-Cas3 system is the attractive genome editing technology, it is hard to apply stem cells and in vivo because of a complex system consisting of multiple proteins and gRNAs.
To tackle this problem, we have developed a delivery system for encapsulating the Cas3 protein and Cascade complex which consisted of the CRISPR-Cas3 system in lentivirus-like particles. Our new delivery system is expected to be applied to the adaptation of the CRISPR-Cas3 system for cells which is difficult to gene introduction, and to gene therapy for triplet repeat disease such as Huntington's disease and myotonic muscular dystrophy.
Industrial property rights 1
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ゲノムDNAに欠失を誘導する方法
堀田 秋津, 奥嵜 雄也, 徐 淮耕, ジー ピーター デビド, 北 悠人, 真下 知士, 吉見 一人
Teaching Experience (On-campus) 1
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ゲノム生物学特論
2023