Updated on 2025/03/14

写真a

 
OKUZAKI Yuya
 
Organization
Graduate School of Bioagricultural Sciences Avian Bioscience Research Center Assistant Professor
Graduate School
Graduate School of Bioagricultural Sciences
Undergraduate School
School of Agricultural Sciences Department of Bioresource Sciences
Title
Assistant Professor
Contact information
メールアドレス
Homepage
https://www.agr.nagoya-u.ac.jp/~abrc/abrc-E/index.html
External link

Degree 1

  1. Doctor (Engineering) （ 2017.12   Nagoya University ） 

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Research Interests 4

  1. transgenic chicken

  2. gene engineering

  3. genome editing

  4. primordial germ cells

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Research Areas 2

  1. Life Science / Genome biology  / genome editing, gene engineering, transgenic birds

  2. Life Science / Animal production science  / genome editing, gene engineering, transgenic birds

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Research History 2

  1. Nagoya University   Graduate School of Bioagricultural Sciences Avian Bioscience Research Center   Assistant Professor

    2022.7

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    Country：Japan

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  2. Nagoya University   Graduate School of Bioagricultural Sciences Avian Bioscience Research Center   Researcher

    2020.4 - 2022.6

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    Country：Japan

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Professional Memberships 4

  1. 日本ゲノム編集学会

  2. 日本動物細胞工学会

  3. 日本分子生物学会

  4. 日本生物工学会

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Papers 16

  1. Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skipping. Reviewed

    Nature communications   Vol. 11 ( 1 ) page： 1334   2020.3

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    Language：English   Publishing type：Research paper (scientific journal)  

  2. CRISPR-Cas3 induces broad and unidirectional genome editing in human cells. Reviewed

    Hiroyuki Morisaka, Kazuto Yoshimi, Yuya Okuzaki, Peter Gee, Yayoi Kunihiro, Ekasit Sonpho, Huaigeng Xu, Noriko Sasakawa, Yuki Naito, Shinichiro Nakada, Takashi Yamamoto, Shigetoshi Sano, Akitsu Hotta, Junji Takeda, Tomoji Mashimo

    Nature communications   Vol. 10 ( 1 ) page： 5302   2019.12

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/s41467-019-13226-x

  3. PRDM14 and BLIMP1 control the development of chicken primordial germ cells. Reviewed

    Yuya Okuzaki, Hidenori Kaneoka, Takayuki Suzuki, Yota Hagihara, Yuki Nakayama, Seitaro Murakami, Yusuke Murase, Atsushi Kuroiwa, Shinji Iijima, Ken-Ichi Nishijima

    Developmental biology   Vol. 455 ( 1 ) page： 32 - 41   2019.11

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    Authorship：Lead author   Language：English  

    DOI： 10.1016/j.ydbio.2019.06.018

  4. Avian Bioresources for Developmental Biology: Chicken and Quail Resources in the United Kingdom, France, and Japan. International journal

    Lindsay Henderson, Yuya Okuzaki, Christophe Marcelle, Mike McGrew, Ken-Ichi Nishijima

    Developmental biology   Vol. 521   page： 1 - 13   2025.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    Biological resources are essential for the research using chickens and quails, particularly in the field of developmental biology. Various lines of chickens and quails with naturally occurring genetic mutations and diverse phenotypes have been developed. In addition, recent advances in establishing the genetic modification of cultured primordial germ cells (PGCs) with DNA transposons and the lentivirus-mediated transduction of PGCs in vivo and DNA transposons have been used to develop various transgenic chicken and quail lines. However, the relatively large body size of chickens and the need to maintain living animals due to the previous lack of reliable frozen stock methods until the development of cultivating methods of PGCs has caused a steady decline in the number of available lines globally. Several research facilities maintain chicken and quail lines and provide them for research purposes. This review describes the three main avian resource sites: The National Avian Research Facility at The Roslin Institute in the United Kingdom, Lyon Transgenic Quail Facility (MeLiS) in France, and Avian Bioscience Research Center at Nagoya University in Japan.

    DOI： 10.1016/j.ydbio.2025.02.001

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  5. Optimized simple culture protocol for inducing mature myotubes from MYOD1-overexpressed human iPS cells. International journal Open Access

    Eiji Wada, Nao Susumu, Yuya Okuzaki, Akitsu Hotta, Hidetoshi Sakurai, Yukiko K Hayashi

    Scientific reports   Vol. 14 ( 1 ) page： 28783 - 28783   2024.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    The forced expression system of MYOD1, a master gene for myogenic differentiation, can efficiently and rapidly reproduce muscle differentiation of human induced pluripotent stem cells (hiPSCs). Despite these advantages of the MYOD1 overexpression system, developed myotubes are relatively immature and do not recapitulate several aspects of striated muscle fibers. Here, we developed a simple optimized protocol using an alternative culture medium for maximizing the advantages of the MYOD1 overexpression system, and successfully improved the formation of multinucleated mature myotubes within 10 days. In this study, we generated hiPSCs derived from healthy donors and an individual with congenial muscular dystrophy caused by LMNA mutation (laminopathy), and compared disease-associated phenotypes in differentiated myotubes generated by the conventional method and by our new optimized culture method. Using our optimized method, abnormal myonuclear shape was pronounced in the patient-derived iPSCs. In addition, abnormal accumulation of the nuclear membrane protein emerin was observed in LMNA-mutant hiPSCs. Our new culture method is expected to be widely applicable as a MYOD1 overexpression model of hiPSC-derived skeletal muscle cells for the analysis of a variety of muscle diseases.

    DOI： 10.1038/s41598-024-79745-w

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  6. PGC-based cryobanking, regeneration through germline chimera mating, and CRISPR/Cas9-mediated TYRP1 modification in indigenous Chinese chickens. International journal Open Access

    Keiji Kinoshita, Kumiko Tanabe, Yoshiaki Nakamura, Ken-Ichi Nishijima, Takayuki Suzuki, Yuya Okuzaki, Shusei Mizushima, Ming-Shan Wang, Sami Ullah Khan, Kaixiang Xu, Muhammad Ameen Jamal, Taiyun Wei, Heng Zhao, Yanhua Su, Feizhou Sun, Gang Liu, Fangxian Zhu, Hong-Ye Zhao, Hong-Jiang Wei

    Communications biology   Vol. 7 ( 1 ) page： 1127 - 1127   2024.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    Primordial germ cells (PGCs) are vital for producing sperm and eggs and are crucial for conserving chicken germplasm and creating genetically modified chickens. However, efforts to use PGCs for preserving native chicken germplasm and genetic modification via CRISPR/Cas9 are limited. Here we show that we established 289 PGC lines from eight Chinese chicken populations with an 81.6% success rate. We regenerated Piao chickens by repropagating cryopreserved PGCs and transplanting them into recipient chickens, achieving a 12.7% efficiency rate. These regenerated chickens carried mitochondrial DNA from female donor PGC and the rumplessness mutation from both male and female donors. Additionally, we created the TYRP1 (tyrosinase-related protein 1) knockout (KO) PGC lines via CRISPR/Cas9. Transplanting KO cells into male recipients and mating them with wild-type hens produced four TYRP1 KO chickens with brown plumage due to reduced eumelanin production. Our work demonstrates efficient PGC culture, cryopreservation, regeneration, and gene editing in chickens.

    DOI： 10.1038/s42003-024-06775-5

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  7. Dual CRISPR-Cas3 system for inducing multi-exon skipping in DMD patient-derived iPSCs Reviewed

    Stem Cell Reports   Vol. 18 ( 9 ) page： 1753 - 1765   2023.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.stemcr.2023.07.007

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  8. Regulatory mechanism of chicken lysozyme gene expression in oviducts examined using transgenic technology. Reviewed

    Yusuke Kojima, Yuya Okuzaki, Ken-Ichi Nishijima, Shuichiro Moriwaki, Seiya Asai, Hidenori Kaneoka, Shinji Iijima

    Journal of bioscience and bioengineering   Vol. 131 ( 4 ) page： 453 - 459   2021.4

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.jbiosc.2020.11.011

  9. Primordial germ cell-specific expression of eGFP in transgenic chickens. Invited Reviewed

    Yota Hagihara, Yuya Okuzaki, Kazuma Matsubayashi, Hidenori Kaneoka, Takayuki Suzuki, Shinji Iijima, Ken-Ichi Nishijima

    Genesis   Vol. 58 ( 8 ) page： e23388   2020.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/dvg.23388

  10. Identification of transgene integration site and anatomical properties of fluorescence intensity in a EGFP transgenic chicken line. Reviewed

    Kaori Tsujino, Yuya Okuzaki, Nobuyuki Hibino, Kazuki Kawamura, Seiji Saito, Yumi Ozaki, Satoshi Ishishita, Atsushi Kuroiwa, Shinji Iijima, Yoichi Matsuda, Kenichi Nishijima, Takayuki Suzuki

    Development, growth & differentiation   Vol. 61 ( 7-8 ) page： 393 - 401   2019.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/dgd.12631

  11. Molecular cloning of chicken TET family genes and role of chicken TET1 in erythropoiesis. Reviewed

    Yuya Okuzaki, Hidenori Kaneoka, Ken-Ichi Nishijima, Seitaro Murakami, Yuki Ozawa, Shinji Iijima

    Biochemical and biophysical research communications   Vol. 490 ( 3 ) page： 753 - 759   2017.8

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bbrc.2017.06.113

  12. Expression of interferon-inducible transmembrane proteins in the chicken and possible role in prevention of viral infections. Reviewed

    Shunsuke Kidani, Yuya Okuzaki, Hidenori Kaneoka, Seiya Asai, Seitaro Murakami, Yusuke Murase, Shinji Iijima, Ken-Ichi Nishijima

    Cytotechnology   Vol. 69 ( 3 ) page： 477 - 484   2017.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s10616-016-9958-1

    Other Link： https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5461238

  13. Characterization of chicken interferon-inducible transmembrane protein-10. Reviewed

    Yuya Okuzaki, Shunsuke Kidani, Hidenori Kaneoka, Shinji Iijima, Ken-Ichi Nishijima

    Bioscience, biotechnology, and biochemistry   Vol. 81 ( 5 ) page： 914 - 921   2017.5

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1080/09168451.2016.1274639

  14. Analyses of chicken sialyltransferases related to O-glycosylation. Reviewed

    Shunsuke Kidani, Hidenori Kaneoka, Yuya Okuzaki, Seiya Asai, Yusuke Kojima, Ken-Ichi Nishijima, Shinji Iijima

    Journal of bioscience and bioengineering   Vol. 122 ( 4 ) page： 379 - 384   2016.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.jbiosc.2016.03.017

  15. Analyses of chicken sialyltransferases related to N-glycosylation. Invited Reviewed

    Yusuke Kojima, Akifumi Mizutani, Yuya Okuzaki, Ken-Ichi Nishijima, Hidenori Kaneoka, Takako Sasamoto, Katsuhide Miyake, Shinji Iijima

    Journal of bioscience and bioengineering   Vol. 119 ( 6 ) page： 623 - 628   2015.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.jbiosc.2014.11.009

  16. Recombinant proteins produced into yolk of genetically manipulated chickens are partly sialylated in N-glycan. Invited Reviewed

    Kazuhiro Yoshida, Yuya Okuzaki, Ken-Ichi Nishijima, Kenji Kyogoku, Takashi Yamashita, Yoshinori Kawabe, Makoto Motono, Masamichi Kamihira, Shinji Iijima

    Cytotechnology   Vol. 65 ( 6 ) page： 985 - 992   2013.12

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    Language：English   Publishing type：Research paper (scientific journal)  

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Presentations 2

  1. Development of the SpCas9 constitutively expressing transgenic chicken International conference

    Yuya OKUZAKI

    Avian Model Systems 11  2023.9.12 

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    Event date： 2023.9

    Language：English   Presentation type：Oral presentation (general)  

    Venue：University of Portsmouth   Country：United Kingdom  

  2. SpCas9発現遺伝子組換えニワトリの作製

    奥嵜雄也

    日本ゲノム編集学会第8回大会  2023.6.7  日本ゲノム編集学会

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    Event date： 2023.6

    Language：English   Presentation type：Poster presentation  

    Venue：タワーホール船堀   Country：Japan  

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KAKENHI (Grants-in-Aid for Scientific Research) 3

  1. インフルエンザパンデミック制御におけるニワトリトランスジェニック技術の展開

    Grant number：24K01268  2024.4 - 2028.3

    日本学術振興会  科学研究費助成事業  基盤研究(B)

    西島 謙一, 高桑 弘樹, 金岡 英徳, 奥嵜 雄也, 内田 裕子, 高桑 弘樹, 金岡 英徳, 奥嵜 雄也, 内田 裕子

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    Authorship：Coinvestigator(s) 

    本申請では、ようやく可能となりつつあるニワトリの遺伝子改変技術を最大限生かして、古くから継続する脅威であるインフルエンザに対する対抗技術を開発することを目指す。遺伝子改変ニワトリを利用することで、改善の余地のあるインフルエンザワクチン生産系を飛躍的に効率化することを目指す。合わせて、鳥インフルエンザの流行にも頑健性を持ってワクチン生産システムを維持できるよう、インフルエンザ耐性ニワトリの育種を進める。

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  2. ゲノム編集技術を用いたインフルエンザウイルス抵抗性ニワトリの作製に向けた研究

    Grant number：22K15027  2022.4 - 2027.3

    科学研究費助成事業  若手研究

    奥嵜 雄也

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    Authorship：Principal investigator 

    Grant amount：\4550000 （ Direct Cost: \3500000 、 Indirect Cost：\1050000 ）

    本研究では、ゲノム編集技術であるCRISPR-Cas9を用い、ニワトリ細胞において鳥インフルエンザウイルスの感染や増殖に必要な宿主遺伝子のスクリーニングを行う。このスクリーニングにより見出されたインフルエンザウイルスに抵抗性を示すと考えられる候補遺伝子を、実際にニワトリ個体でゲノム編集することで、インフルエンザ抵抗性ゲノム編集ニワトリ系統の作製を目指す。

  3. Development of CRISPR-Cas3 delivery system using virus-like particles

    Grant number：20K15776  2020.4 - 2022.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Early-Career Scientists

    OKUZAKI Yuya

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    CRISPR-Cas3 system is the novel genome editing technology which can efficiently introduce large-scale deletion on the genome. Although CRISPR-Cas3 system is the attractive genome editing technology, it is hard to apply stem cells and in vivo because of a complex system consisting of multiple proteins and gRNAs.
    To tackle this problem, we have developed a delivery system for encapsulating the Cas3 protein and Cascade complex which consisted of the CRISPR-Cas3 system in lentivirus-like particles. Our new delivery system is expected to be applied to the adaptation of the CRISPR-Cas3 system for cells which is difficult to gene introduction, and to gene therapy for triplet repeat disease such as Huntington's disease and myotonic muscular dystrophy.

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Industrial property rights 1

  1. ゲノムDNAに欠失を誘導する方法

    堀田 秋津, 奥嵜 雄也, 徐 淮耕, ジー ピーター デビド, 北 悠人, 真下 知士, 吉見 一人

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    Application no：JP2019048426   Date applied：2019.12

    Publication no：WO2020-122104   Date published：2020.6

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Teaching Experience (On-campus) 1

  1. ゲノム生物学特論

    2023

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